Effects of Mongolian astragalus saponin on neuroinflammation and intestinal microflora disorder induced by lead exposure in developing rats

doi:10.11958/20221821

Abstract

Abstract:

Objective To investigate the effect of Mongolian astragalus saponin on neuroinflammation and intestinal microbiota disorder caused by lead exposure in developing SD rats. Methods Forty 4-week-old Sprague Dawley rats were randomly divided into the control group (CG), the lead exposure group (LE) and the saponin intervention groups [high-dose (SH), medium-dose (SM) and low-dose (SL)]. After 4 weeks of corresponding intervention, blood lead content of rats was detected by hydride generation atomic fluorescence spectrometry. Levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) in hippocampal tissue were detected by enzyme linked immunosorbent assay. Changes of intestinal microbiota composition in cecal contents were detected by 16S rDNA sequencing. Results Compared with the CG group, blood lead levels were increased in other groups(P<0.05), levels of TNF-α, IL-6 and IL-1β in hippocampus were increased in the LE group, and the α and β diversity of intestinal flora were significantly changed in the LE group. Shannon index and Simpson index were decreased, and relative abundance of Romboutsia, Blautia and Lachnospiraceae_UCG-001 was decreased. The relative abundance of Ruminococcus and Prevotellaceae_UCG-001 was increased (P<0.05). Compared with the LE group, blood lead levels were decreased in the SL group and the SM group (P<0.05). Hippocampal IL-6 level in the SL group, the level of IL-1β in the SM group, levels of TNF-α and IL-6 in the SM group and the SH group were decreased (P<0.05). β diversity in the SL group and the SM group, and α diversity in the SM group were significantly improved, and Simpson index was increased in the SM group (P<0.05). The relative abundance of Ruminococcus and Prevotellaceae_UCG-001 decreased in the SM group and the SH group (P<0.05). The relative abundance of Romboutsia, Lachnospiraceae_UCG-001 and Blautia were significantly increased in the SM group and the SL group (P<0.05). The abundance of Romboutsia increased more significantly in the SM group than that in the SH group (P<0.05). Conclusion Mongolian astragalus saponins can effectively inhibit neuroinflammatory response and intestinal flora disorder in hippocampus of lead-induced developing rats, and the effect of medium dose intervention is obvious.

Key words: lead poisoning, nervous system, childhood, gastrointestinal microbiome, astragaloside, astragalus membranaceus bungevar.mongholicus(bunge) P.K.Hsiao, inflammatory factor

CLC Number:

R598.11

Figures/Tables 8

References 23

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组别	体质量/g	血铅/（μg/L）
CG组	236.81±18.79	0.292±0.030
LE组	215.87±15.02	6.644±1.899^a
SL组	226.97±14.71	3.916±0.779^ab
SM组	237.29±12.16	3.439±1.447^ab
SH组	230.81±24.99	4.527±0.829^a
F	2.541	111.180^＊

组别	体质量/g	血铅/（μg/L）
CG组	236.81±18.79	0.292±0.030
LE组	215.87±15.02	6.644±1.899^a
SL组	226.97±14.71	3.916±0.779^ab
SM组	237.29±12.16	3.439±1.447^ab
SH组	230.81±24.99	4.527±0.829^a
F	2.541	111.180^＊

组别	TNF-α	IL-6	IL-1β
CG组	231.55±55.78	136.81±23.92	334.41±93.85
LE组	334.44±53.89^a	193.81±25.45^a	477.92±42.93^a
SL组	284.79±47.28	167.87±10.25^b	396.67±107.62
SM组	243.01±54.54^b	157.01±29.73^b	379.65±80.07^b
SH组	264.31±56.97^b	156.69±24.69^b	402.22±63.52
F	4.559^＊	6.150^＊	2.935^＊

组别	TNF-α	IL-6	IL-1β
CG组	231.55±55.78	136.81±23.92	334.41±93.85
LE组	334.44±53.89^a	193.81±25.45^a	477.92±42.93^a
SL组	284.79±47.28	167.87±10.25^b	396.67±107.62
SM组	243.01±54.54^b	157.01±29.73^b	379.65±80.07^b
SH组	264.31±56.97^b	156.69±24.69^b	402.22±63.52
F	4.559^＊	6.150^＊	2.935^＊

组别	Shannon指数	Simpson指数	chao1指数
CG组	3.78±0.74	0.79±0.08	334.39±48.60
LE组	2.93±0.62^a	0.61±0.12^a	319.18±38.18
SL组	3.12±0.53^a	0.69±0.10	356.63±66.34
SM组	3.24±0.60	0.73±0.10^b	323.82±38.67
SH组	2.98±0.37^a	0.71±0.14	325.74±31.39
F	2.788^＊	2.730^＊	0.825