Abstract: 0bjective   To study the expression of Fas in hippocampus of cerebral ischemia-reperfusion rat after monosialoganglioside was injected. Methods   72 SD rats were randomly divided into the sham group, ischemia-reperfusion group(I/R), monosialoganglioside group(GM1) at different time points (6h, 12h, 24h, 3d) after reperfusion on average. The expression of Fas in hippocampus of all groups was examined by immunochemistry and western blot methods. Result   There is a little of Fas positive expression in hippocampus of the rats in sham group, and the positive expression of Fas was increased significantly after reperfusion (P< 0.05), to the peak at 3d, compared with the rats of the I/R group at the same time point, the positive expression of Fas decreased significantly in GM1 group (P< 0.05) ; and the same result was obtain by western blot method. Conclusion   one of the mechanisms for monosialoganglioside participate the pathogenesis of cerebral ischemia-reperfusion injury might be downregulate the expression of Fas.

Key words: reperfusion injury, G(M1) ganglioside, monosialoganglioside, Hippocampus, Fas ligand protein, rats, Sprague-Dawley