Abstract: 【Abstract】Objective :To investigate the effect of hypoxia on MICA expression in GES-1 its mechanism. Methods :Hypoxia culture model in vitro was established to test the expression of MICA in GES-1 cell line. After cultured in hypoxia circumstance for a given period of time (12hours ,24hours ,72hours ) ,MICA expression was tested in mRNA level and protein level by RT - PCR and immunocytochemistry analysis . Results: Cell survival was affected a little within 20 hours under hypoxia. MICA expressions increased after treatment of hypoxia. MICA protein expressions increased equally with mRNA confirmed by immunocytochemistry and RT - PCR and its positive correlation was found by Pearson product - moment correlation analysis. Conclusion :MICA gene and protein can be induced remarkably byhypoxia in GES-1.

Key words: Hypoxia, MICA, GES-1