Study on the mechanism of transcription factor EB in autophagy of aging cardiomyocytes

doi:10.11958/20220920

Abstract

CLC Number:

R542.2

Figures/Tables 10

References 27

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基因名称	引物序列（5'→3'）
外引物	上游：TATATTTGGATGGTTTTTTGTGG 下游：TAACCCTAACTAACCCAACATTCAT
甲基化引物	上游：GATAAAAGGGTAAGATTTAGGTCGT 下游：TCCCTCCATATTTACATACTTCGTA
非甲基化引物	上游：ATAAAAGGGTAAGATTTAGGTTGT
非甲基化引物	下游：TCCCTCCATATTTACATACTTCATA

基因名称	引物序列（5'→3'）
外引物	上游：TATATTTGGATGGTTTTTTGTGG 下游：TAACCCTAACTAACCCAACATTCAT
甲基化引物	上游：GATAAAAGGGTAAGATTTAGGTCGT 下游：TCCCTCCATATTTACATACTTCGTA
非甲基化引物	上游：ATAAAAGGGTAAGATTTAGGTTGT
非甲基化引物	下游：TCCCTCCATATTTACATACTTCATA

组别	LC3B-Ⅱ/Ⅰ	p62
Ad-GFP组	2.149±0.145	0.421±0.062
Ad-GFP+H/R组	4.135±0.246	0.114±0.021
Ad-TFEB组	1.144±0.112^a	0.963±0.077^a
Ad-TFEB+H/R组	1.880±0.104^b	0.261±0.040^b
F	186.600^＊＊	138.500^＊＊
sh-NC组	0.975±0.233	0.741±0.034
sh-NC+H/R组	1.165±0.005	0.348±0.064
sh-TFEB组	2.222±0.140^c	0.307±0.074^c
sh-TFEB+H/R组	4.248±0.259^d	0.169±0.027^d
F	191.900^＊＊	63.040^＊＊

组别	LC3B-Ⅱ/Ⅰ	p62
Ad-GFP组	2.149±0.145	0.421±0.062
Ad-GFP+H/R组	4.135±0.246	0.114±0.021
Ad-TFEB组	1.144±0.112^a	0.963±0.077^a
Ad-TFEB+H/R组	1.880±0.104^b	0.261±0.040^b
F	186.600^＊＊	138.500^＊＊
sh-NC组	0.975±0.233	0.741±0.034
sh-NC+H/R组	1.165±0.005	0.348±0.064
sh-TFEB组	2.222±0.140^c	0.307±0.074^c
sh-TFEB+H/R组	4.248±0.259^d	0.169±0.027^d
F	191.900^＊＊	63.040^＊＊

组别	TFEB
组别	mRNA	蛋白
H/R组	1.111±0.145	0.443±0.041
DC-05组	1.031±0.125	0.466±0.018
TFD组	1.150±0.216	0.478±0.045
NA组	2.386±0.132^a	0.929±0.139^a
F	49.590^＊＊	28.020^＊＊

Study on the mechanism of transcription factor EB in autophagy of aging cardiomyocytes

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组别	TFEB
组别	mRNA	蛋白
sh-NC组	0.391±0.021	0.244±0.014
sh-NC+H/R组	0.306±0.055	0.081±0.005
sh-DNMT3b组	1.182±0.061^a	0.548±0.044^a
sh-DNMT3b+H/R组	0.595±0.031^b	0.218±0.065^b
F	229.500^＊＊	72.900^＊＊

组别	TFEB
组别	mRNA	蛋白
sh-NC组	0.391±0.021	0.244±0.014
sh-NC+H/R组	0.306±0.055	0.081±0.005
sh-DNMT3b组	1.182±0.061^a	0.548±0.044^a
sh-DNMT3b+H/R组	0.595±0.031^b	0.218±0.065^b
F	229.500^＊＊	72.900^＊＊