Abstract: Effect of Maternal Lipopolysaccharide Stimulation on the Activation of Renin-Angiotensin System in New born Offspring of Rats HAO Xueqin College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China Abstract Objective：To explore the effect of maternal exposure to lipopolysaccharide (LPS) on the activation of renin-angiotensin system in new born offspring of rats. Methods： Twelve Sprague-Dawley rats were randomly divided into two groups (n=6 for each grou)，control group and LPS group. On the pregnant day 8, 10 and 12, rats of control group and LPS group were given intraperitoneally with normal saline 0.5 ml or LPS 0.79 mg/kg, respectively. The levels of renal rennin，angiotensin converting enzyme (ACE) mRNA and angiotensin Ⅱ type Ⅰ receptor（AT1-R) protein were detected by real-time PCR and Western blotting, respectively. The expression of angiotensin II in kidney was evaluated by immunohistochemical staining. Results：Compared with control group, the renin mRNA expression was significantly decreased，and the AT1-R protein expression was increased，in offspring of LPS group (P＜0.01). There was no significant difference in ACE mRNA expression between two groups (P＞0.05). The Angiotensin II-positive cells in kidney of LPS-treated offspring were lower compared with those of control group (P＜0.01). Conclusion：Maternal lipopolysaccharide stimulation suppresses renal renin-angiotensin system in new born offspring of rats.

Key words: inflammation, lipopolysaccharide, offspring, hypertension