Abstract:

[Abstract]   Objective   To discuss the influence and significance of stromal cell-derived factor1(SDF -1) and its
specific receptor CXC chemokine receptor4(CXCR4) in proliferation, migration and invasion ability of SW480colorectal cancer cells.  Methods   The colorectal cancer cell line SW480in logarithmic phase was divided into four groups: control group (with no any processing), SDF-1group (added100μg/L SDF-1), SDF-1＋1mg/L AMD3100mixed group (added1mg/L AMD3100for 2hours, then added100μg/L SDF-1) and AMD3100group (added1mg/L AMD3100). Immunohisto?chemistry method was used to detect the protein expression of CXCR4in SW480cells. The expression of CXCR4mRNA in SW480cells was detected by RT-PCR before and after SDF-1and AMD3100treatment. MTT assay and transwell chamber were used to test the changes of proliferation, migration and invasion ability of SW480cells before and after SDF-1and AMD3100treatment.   Results   The result of immunohistochemistry showed that CXCR4protein was expressed in SW480 cells (positive rate=80%). CXCR4mRNA was expressed in SW480cells. The expression of CXCR4mRNA was up-regulat?ed by SDF-1(100μg/L), which could be inhibited by AMD3100(1mg/L). The proliferation activity was higher in SDF-1group (0.847±0.039) compared to that in control group (0.624±0.011) and SDF-1＋AMD3100mixed group(0.607±0.016).The proliferation activity was lower in AMD3100group (0.456±0.031) than that in control group and SDF-1＋AMD3100 mixed group (F=108.03, P＜0.05). The number of transmembrane cells was more in SDF-1group (98.7±5.8, 33.7±6.2) than that in control group (21.0±2.2, 6.1±2.3), SDF-1＋1mg/L AMD3100mixed group (18.5±8.4, 8.5±2.8) and AMD3100group (12.1±3.2, 2.1±1.0) detected by transwell chamber experiment. However, there were no statistical differences between three groups.   Conclusion   The biological axis SDF-1/CXCR4 can promote the proliferation, migration and invasion in colorectal cancer cell line SW480.

Key words: intestinal neoplasms, intestine large, chemokine CXCL12, receptors, CXCR4, cell proliferation, migration movement, cell migration, Cell invasion, AMD3100