Abstract: Objective To investigate the HPV infection, genotypes and related high risk factors among 459 women conducted health examination. Methods 459 cervical cytology specimens were detected with nest PCR and pyrosequencing，questionnaires were collected simultaneously and the risk factors of HPV infection were analyzed . Results 82 of 465 cases (17.9%) were infected with HPV. 7 kinds of HR-HPV were found. HPV 16 were the most common (9.8%), HPV58 (7.0%), HPV18 (5.2%) orderly. The rate of single, duplex, multiplex infection was 9.6%, 4.8%, 1.5% respectively. HPV infection was closely correlated with drinking，low economy condition，having multiple sexual partners，sexually active and cervical erosion (P＜0.05). Conclusion HPV DNA genotyping can provide important reference for HPV screening at early time and the application of HPV vaccines; it also contributes to provide significant evidence for the prevention, diagnosis, and treatment of cervical cancer at early stage for high risk population.

Key words: papillomaviridae, uterine cervical neoplasms, carcinoma, genotype, risk factors, polymerase chain reaction, pyrosequencing