Abstract: Abstract: Objective To investigate the cytocompatibility of the liching liquids of Co-Cr alloy and Ti alloy on human gingival fibroblasts (HGF). Methods The HGF were treated in vitro with leaching liquids of Co-Cr alloy and Ti alloy, re⁃ spectively. The DMEM cell medium containing 10% fetal bovine serum was served as a negative control. The viability of HGF treated by two dental alloys were evaluated by means of MTT, and the contents of intracellular reduced glutathione (rGSH) were assayed by kits. The tumor necrosis factor-α (TNF-α) contents were determined in the culture supernatant by ELISA in two groups. The effects of these alloys on the expression of caspase-3 were examined by real time-PCR method. Results Compared with the control group, HGF treated with Co-Cr alloy leaching liquids showed a lower viability (P < 0.05), while Ti alloy leaching liquid promoted the proliferation of HGF. In Co-Cr alloy group, the rGSH content was signifi⁃ cantly decreased (P < 0.05), while TNF-α content was significantly increased (P < 0.05) compared with control group. There were no significant differences in rGSH and TNF-α contents between the Ti alloy group and control group (P > 0.05). The ex⁃ pression of caspase-3 was significantly higher in Co-Cr alloy group than that of control group (P < 0.05), while there was no significant difference in the expression of caspase-3 between Ti alloy group and control group. Conclusion Results suggest that Co-Cr alloy possesses cytotoxicity, while there is better cell compatibility for Ti alloy.

Key words: Chromium alloys, Titanium, glutathione, tumor necrosis factor-alpha, caspase 3, Co-Cr alloy, Ti alloy, hu? man gingival fibroblast, cytocompatibility