Abstract: Abstract: Objective　To investigate the expression of long non-coding RNA (lncRNA) brain cytoplasmic RNA 1 (BCYRN1) in lung adenocarcinoma cell lines A549 and H1299 and its effects on cell cycle, apoptosis, invasion and migration, then to analyze the relationship between the expression of BCYRN1 and clinicopathological features and prognosis of patients with lung adenocarcinoma. Methods　Normal lung epithelial cells BEAS-2B and lung adenocarcinoma cells A549 and H1299 were cultured. Real-time quantitative polymerase chain reaction (qPCR) was used to detect the expression level of BCYRN1 in normal cells and lung adenocarcinoma cells. BCYRN1 down-regulation and control plasmid were transfected into H1299 lung adenocarcinoma cells, respectively. The cell cycle and apoptosis were analyzed by flow cytometry. Changes of cell invasion and migration ability were detected by scratch test and Transwell test. Combined with the Cancer Genome Atlas (TCGA) database, the correlation between BCYRN1 expression and clinicopathological characteristics of patients with lung adenocarcinoma was analyzed. Cox regression was used to analyze the relationship between BCYRN1 expression and patient prognosis by using the lncRNAs from cancer arrays (lnCAR) database. Results　The expression level of BCYRN1 was significantly higher in lung adenocarcinoma cells than that in normal lung epithelial cells (P＜0.05). Flow cytometry analysis indicated that S phase arrest and the proportion of cells in the apoptotic phase increased in the BCYRN1 knockdown cells. The scratch test and Transwell test showed that the invasion and migration of H1299 cells reduced in BCYRN1 knockdown group. Bioinformatics analysis showed that the expression of BCYRN1 was associated with N stage and tumor stage (P＜0.05), and the overall survival of patients with high BCYRN1 expression was significantly shortened. Conclusion BCYRN1 is highly expressed in lung adenocarcinoma cells. BCYRN1 knockdown inhibits the invasion and migration of lung adenocarcinoma and causes cell cycle arrest. The high expression of BCYRN1 is associated with N stage and tumor stage of lung adenocarcinoma. The prognosis of patients with high expression of BCYRN1 is poor.

Key words: long noncoding RNA, lung neoplasms, adenocarcinoma, computational biology, cell proliferation, cell movement, BCYRN1