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15 May 2010, Volume 38 Issue 5 Previous Issue    Next Issue
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实验研究
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临床论丛
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实验研究

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Effect of Telmisartan on The Expression of MCP-1 in OLETF Rats With Insulin Ressitance 2010, 38 (5):  0-0 .  Abstract ( 2006 )   PDF (1381KB) ( 1945 )   Abstract Objective To investigate the changes of MCP-1 in OLETF rats with Insulin Resistance and whether angiotensin II type／(AT1)receptor blocker，Telmisartan has a protective effect by influencing MCP-1 expression．Methods 22 male OLETF rats were fed with high-fat diet for 32 weeks to establish the insulin resistance model with hyperlipidemia。the 20 male OLETF rats with insulin resistance model were randomly assigned into two groups:①the model group,in which the rats were fed with high-diet;②the Telmisartan group,in which the rats were fed with high-diet and Telmisartan .After 16 weeks of drug intervention,MCP-1,fasting blood glucose,fasting insulin,FFA and lipid level in plasma were measured.Results After the OLETF rats were treated for 16 weeks with Telmisartan , Telmisartan can improve insulin resistance and lipid metabolism .the serum MCP-1 was significantly lower than that of the model group(p<0.05).Conclusion Telmisartan can improve IR、blood glucose and the mechanism might be that it could inhibit the MCP-1 expression in serum. Related Articles | Metrics

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Effects of tumor necrosis factor-α on expression of adiponectin and inflammation cytokines 2010, 38 (5):  0-0 .  Abstract ( 1358 )   PDF (1793KB) ( 2062 )   Objective:To investigate the effect of TNF–αin the pathophysiological course of obesity-inflammation-insulin resistance(IR)-type 2 diabetic mellitus(T2DM).Methods: 3T3-L1 preadipocytes were cultured and induced into mature adipocytes.Observed the expressions of ADPN mRNA,PPAR-γ mRNA in the differential course of the adipocytes. Observed the expressions of ADPN mRNA,PPAR-γ mRNA, TNF–αmRNA, IL-6 mRNA and IL-1β mRNA in mature adipocytes which were treated with high concentration （20ng/mL）of the recombinant TNF–αunder diverse phase.Results:The levels of ADPN mRNA,PPAR-γ mRNA expressions were upregulated gradually within the differential course of 3T3-L1 adipocytes.The results of the genes’ expressions in adipocytes treated by TNF–α（20ng/mL）were as following: The expressions of ADPN mRNA and PPAR-γ mRNA decreased with time-dependent manner.The expression of TNF–αmRNA and IL-6 mRNA increased significantly. The expression of IL-1β mRNA didn’t show change in statistics.Conclusions: The inflammation hypothesis on etiologic mechanism of type 2 DM was studied at molecular level in this work, TNF–αplays an important role in the course of obesity-inflammation-IR-T2DM. Related Articles | Metrics

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Effects of Probucol combined with Simvastatin on Endothelial Function in Patients with Acute Coronary Syndrome 2010, 38 (5):  0-0 .  Abstract ( 821 )   PDF (1299KB) ( 1981 )   Objective: The study investigate probucol combined with simvastation endothelial function in patients with acute coronary syndrome(ACS). Method :A total of 60 ACS patients randomly received standard therapy plus simvastatin (A,n=30) or standard therapy plus probucol combined with simvastatin (B,n=30). Plasma oxidized low-density lipoprotein(ox-LDL) and nitric oxide(NO) were measured.The brachial arterial hyperemia-induced flow mediated dilation(FMD) mediated vasodilatations were measured by high resolution ultrasound. These variables were analyzed before and after 3 months. Results: the difference of Plasma ox-LDL before and after the treatment between the two groups were lower in A group than in B group, had statistically significant(t=30.681,P<0.001). the difference of Plasma NO before and after the treatment between the two groups were rised in A group than in B group ， had statistically significant(t=17.3403,P<0.001).the difference of FMD before and after the treatment between the two groups were rised in A group than in B group ， had statistically significant(P<0.001).Conclusion: Simvastatin combined with ntioxidant probucol therapy could improve endothelial function in patients with acute coronary syndrome. Related Articles | Metrics

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Susceptibility-weighted imaging for the clinic value of diffuse axonal injury 2010, 38 (5):  0-0 .  Abstract ( 753 )   PDF (1094KB) ( 1990 )   【Abstract】Objective: To study the diagnostic value of susceptibility weighted imaging(SWI)in the cerebral microhemorrhage with DAI,To compare the sensitivity to detect microhemorrhage between T2* and SWI sequence．Methods: Twenty cases with cerebral microhemorrhage of DAI underwent T2* and SWI sequence. Results: Twenty cases of microhemorrhage DAI，the lesions showed that the veins show ratings on SWI were 2.8,2.8；T2 * for the 2.0,2.3； T2 for 2.2,0； T1 as 1.7,0. SWI respectively with T2 *, T2, T1 rating results, the difference was statistically significant (P <0.01). All lesions bleeding SWI sequences were obtained clearly showed that the SWI to detect an average of 76 ± 52 of the mixed low signal intensity, while in T2 *-weighted imaging, compared with 21 ± 19. (P <0.01, paired t test)Concusion: SWI was superior to the conventional T2* for cerebral microhemorrhage with DAI. 【Key word】 susceptibility-weighted imaging, diffuse axonal injury, microhemorrhage Related Articles | Metrics

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Analysis of Epidemiological Feature and relevant risk Factors of Metabolic Syndrome among policeman in Tianjin 2010, 38 (5):  0-0 .  Abstract ( 787 )   PDF (1278KB) ( 2071 )   Abstact Objective: To investigate the epidemiological feature and relevant risk factors of metabolic syndrome among policeman in Tianjin，our reseach aims to provide the scientific study for making preventive and control measures of metabolic syndrome among policeman in Tianjin .Methods: Adopting cross-section survey，we investigated 4160 police officers in Tianjin and the risk factors of MS were analyzed with the logistic regression.Results: The prevalence of metabolic syndrome among policemen in Tianjin is 17.52% and standardization prevalence is 15.16%.Relelvant risk factors of MS included age(OR=1.329)，female(OR=1.927)，diabetes family history(OR=2.356)，smoking(OR=3.724) and obesity(OR=1.461).Conclusion: The prevalence of metabolic syndrome among policemen in Tianjin is high.We should make some effective measures against MS in the high-risk population． Related Articles | Metrics

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Study on D-dimer in patients of systemic lupus erythematosus treated with ginkgo leaf injection. 2010, 38 (5):  0-0 .  Abstract ( 812 )   PDF (1078KB) ( 1879 )   Abstract Objective To study the relation of the level of the D-dimer and ginkgo leaf injection in patients of SLE. Methods The level of the D-dimer were tested by immunoenhancement before and after the treatment with ginkgo leaf injection, meanwhile the control groups were established.. Results Before the treatment, the D-dimer level of patients in both SLE groups were much higher than that of patients in health controls（t‘=7.342 , P<0.001；t’= 7.033 , P< 0.001）； The D-dimer level after the treatment had significant decrease in both SLE groups（t=2.411，0.02 Related Articles | Metrics

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The expression and significance of NF-κB p65 in endometrioid carcinoma and its relationship with ERα 2010, 38 (5):  0-0 .  Abstract ( 1238 )   PDF (1044KB) ( 1923 )   We used immunohistochemical SP methods to study the expression of NF-κB p65 and ERα in 10 cases of normal endometrium, 60 cases of endometrial hyperplasia, and 20 cases of endometrioid adenocarcinoma, aiming to investigate the expression of NF-κB p65 in different tissues of endometrial epithelial cells and its relationship with ERα. We observed that the expression of NF-κB p65 gradually raised from normal endometrium、endometrial hyperplasia to endometrioid adenocarcinoma , with significant difference among these three groups（p<0.05）; and the expression of ERα gradually reduced from simple、complex、atypical hyperplasia to endometrioid adenocarcinoma, with significant difference among them（p<0.05）. The overexpression of NF-κB p65 and the expression of ERα in endometrioid adenocarcinoma showed negative correlation （r= -0.491，p=0.028）. In the end, we concluded that the increased expression of NF-κB p65 may play an important role in endometrioid carcinogenesis and its development, there maybe a molecular mechanism of cross-talk between NF-κB p65 and ERα. Related Articles | Metrics

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Pilot Study of Expression of CCR7,MMP-9 and VEGF in T-cell Non-Hodgkin's lymphomas 2010, 38 (5):  0-0 .  Abstract ( 1362 )   PDF (9143KB) ( 1777 )   Pilot Study of Expression of CCR7,MMP-9 and VEGF in T-cell Non-Hodgkin’s Lymphomas WANG Sheng-Yi, ZHAO Guo-Fan, YANG Jing, BAI Xue, ZHANG Yun-Xiao, XU Xiu-Li Department of Pathology, Tianjin Medical University, Tianjin 300070, China ABSTRACT Objective: To investigate the expression and clinical significance of Chemokine C Receptor-7（CCR7）,Matrix Metalloproteinase-9(MMP-9) and Vascular Endothelial Growth Factor(VEGF)in T-cell non-Hodgkin’s Lymphomas(T-NHL).Methods: Specimens of 41 T-NHL cases and 19 reactive lymphoid hyperplasia cases were collected along with their clinicopathologic data. The expression of CCR7,MMP-9 and VEGF were detected using immunohistochemical staining and were then used to study the correlation between the expression and clinicopathologic factors. Results:The expression of CCR7,MMP-9 and VEGF were high in T-NHL group while low in the control group and the overexpression rates in T-NHL group were 85.37%,90.24%,56.10%, respectively.There was a significant difference in the overexpression rates of CCR7 and MMP-9 betwween the cases with more than one lesions and with single lesion, 92.86% vs 69.23% (Pc=0.001)，92.86% vs 84.62% （Pm=0.041），respectively. There was also a significant difference in the overexpression rates of MMP-9 between the groups classified according to their size(smaller or greater than 3cm),81.25% vs 96.00%(P=0.024).There was no correlation among the expression of CCR7, MMP-9 and VEGF.Conclusion: Overexpression of CCR7, MMP-9 and VEGF were detected in T-cell non-Hodgkin’s Lymphomas and the overexpression of these protein are unfavorable factors influencing prognosis of T-NHL patients. Related Articles | Metrics

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A Clinical Study of The Therapeutic Effect of Hyperbaric Oxygen in Patients with Severe Traumatic Brain Injury 2010, 38 (5):  0-0 .  Abstract ( 1242 )   PDF (962KB) ( 1878 )   Objective：To investigate the role of hyperbaric oxygen therapy in improving the outcome of patients with severe traumatic brain injury. Methods：Selected 60 cases of traumatic brain injury patients， whose GCS score were between 3-8 when registered，all of them were practiced surgical treatment， and then were randomly divided into two groups. Patients of groupⅠ(30 cases)suffered simple drug treatment， the rest of the patients in group Ⅱ were carried out not only drug treatment， but also hyperbaric oxygen therapy after the tenth day. Results：By comparing GCS score and ambulatory EEG monitoring， patients in group Ⅱ had faster restoration of outcome than the ones in groupⅠ after 30 days’ therapy. We observed that the targets of the two groups prompted visible difference. Conclusion：Hyperbaric oxygen therapy can improve the degree and rate of recovery of outcome in patients with severe traumatic brain injury. The patients in group Ⅱ had better outcome than group Ⅰ. Related Articles | Metrics

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The changes of plasma DD and ATШ levels in patients with acute exacerbations of COPD and their relationship with the outcome of the disease , 2010, 38 (5):  0-0 .  Abstract ( 1729 )   PDF (1214KB) ( 1800 )   Objective The purpose of this article was to investigate the changes in D-dimer(DD) and AntithrombinШ(ATШ) and their relationship with the outcome in patients with acute exacerbations of chronic obstructive pulmonary disease (AECOPD). Methods The plasma levels of ATШ and DD were measured in patients with AECOPD before and after 7 days of treatment, and in control subjects. The patients were divided into recover group and worsen group. The plasma levels of ATШ and DD were also compared in the two groups. Results In 38 patients of AECOPD，the level of DD was （0.98±0.80）mg/L，which is significantly higher than in control subjects；ATШ was （77.92±12.61）%，which is significantly lower than in control subjects（P<0.01）. In recover group，the levels of DD decrease from（1.07±0.83）mg/L to（0.62±0.42）mg/L and ATШ increase from （78.20±12.30）% to（87.69±8.34）% with the disease；In worsen group ，DD increase from（0.82±0.76）mg/L to（1.95±1.30）mg/L and ATШ decrease from （77.38±13.67）% to（71.38±10.44）%（P<0.01）. Conclusion The levels of DD increase and ATШ decrease significantly during AECOPD. They are relative with the severity of the disease. The alternation of ATШ and DD may be a useful index in evaluation the severity of the disease and effect of therapy. They are relative with the outcome of AECOPD. Related Articles | Metrics

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The Clinical Study of the Senile Sclerotic Glaucoma 2010, 38 (5):  0-0 .  Abstract ( 1114 )   PDF (1011KB) ( 1901 )   Abstract: Objective: To study the relationship betwween the senile sclerotic glaucoma (SSG) and aging. Methods: Ten years special clinical study about SSG was performed, and we studied the relations between the SSG, primary open angle glaucoma (POAG) and aging. Results: Forty cases were diagnosed SSG in the end including 26/42（61%）in group A, 6/22（27.3%）in group B, 8/22（36.4%）in group C. Conclusion: The prevalence of SSG will increase with aging population in the world. Related Articles | Metrics

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nPKC and cPKC Modulate Translocation of Glucose Transporter 4 in Skeletal Muscle Cells 2010, 38 (5):  0-0 .  Abstract ( 1277 )   PDF (1232KB) ( 1839 )   nPKC and cPKC Modulate Translocation of Glucose Transporter 4 in Skeletal Muscle Cells LI Jinru，NIU Wenyan，CHU Guilan Basic Mdical College, Tianjin Medical University,Tianjin 300070, China Abstract Objective: To evaluate the effects of novel protein kinase C (nPKC) and conventional protein kinase C(cPKC) on modulation of translocation of glucose transporter 4 (GLUT4) in skeletal muscle cells. Methods: To determinate the level of GLUT4myc translocation on cellular surface by treatment with PKC activator PMA and PKC inhibitors Go6976, Go6983 in C2C12GLUT4myc myotubes. Results: Acute stimulation of PMA can significantly increase the translocation of GLUT4myc, Go6976, Go6983 inhibited the translocation of GLUT4myc in different degrees. Chronic stimulation of PMA partly inhibited the translocation of GLUT4myc on cellular surface. Conclusion: Activation of nPKC or cPKC enhance translocation of GLUT4 in Skeletal muscle cells. Related Articles | Metrics

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Effects of H102 on related protein of synaptophysin of APP trangenic mouse 2010, 38 (5):  0-0 .  Abstract ( 1168 )   PDF (1805KB) ( 2102 )   [ABSTRACT] AIM To observe the effects of H102 on related protein of synaptophysin of APP trangenic mouse. METHODS The APP transgenic mice were radomly divided into three groups which are modle group ,H102 treatment group, and the control group which of C57BL/6J mice with the same age and background . Modle geoup and normal control group were treated with saline solution while the H102 treatment group was treated with compounds (80μmol/L H102 in saline solution ),med icine or saline was infused in lateral cerebral ventricle every day.after flished that do water maze testing Immunohistichemical stain and westen blot were done with synapse,PSD-95, shank1.RESULTS [1]Morris water maze test showed that the mean escape latent period(MELP) ,the time of the mice across the flat, and the time of traversing through flat roof,and the time which the mice stay in the third space,were was obviously shorten after H102 treatment compare with the modle group（P<0.01),and similer to the nomal group(P>0.05).[2]The content of synapse,PSD-95 and shank1 were highter in H102 treatment group than in modle group (P<0.01)and simile to the nomal group.CONCLUSION H102 can improve the APP trangenic mouse’ ability of remember obviously,and the content of synapse,PSD-95 and shank1 in APP trangenic mouse’ briarn become higher.H102 is a greate and effective durg which will be treated with AD. Related Articles | Metrics

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Relationship between serum uric acid levels and the severity of angiographic coronary artery disease in patients with CAD 2010, 38 (5):  0-0 .  Abstract ( 798 )   PDF (1542KB) ( 1914 )   Objective To evaluate the relationship between serum uric acid(SUA) levels and the severity of angiographic coronary artery disease in patients with CAD. Methods 474 consecutive patients underwent coronary angiography were selected according to inclusion criteria. Of them, 366 cases were diagnosed as CAD, 108 cases were Non-CAD. Patient with the lumen diameter less than 50% at least in one vessel according to coronary Angiography were diagnosed as CAD. The severity of angiographic CAD was assessed by:1.the number of diseased vessels;2. the CAG Gensini score. The SUA level of men>420μmol/L or women>357μmol/L were diagnosed as hyperuricemia (HUA). Results 1.The SUA levels of CAD patients (317.40 90.14 μmol/L) were higher than those in Non-CAD patients (267.59 73.97μmol/L) (P<0.05).The SUA levels of female CAD patients (293.87 79.88μmol/L) were higher than those in female Non-CAD patients (244.82 66.76 μmol/L)(P<0.05).The SUA levels of male CAD patients (324.96 92.06 μmol/L) were higher than those in male Non-CAD patients (298.28 72.74 μmol/L) (P>0.05).The percentage of HUA in CAD patients(21.9%)were significantly higher than those in Non-CAD patients(6.5%)(P<0.05).The percentage of HUA both in male and female CAD patients(men 19.9%;women 28.1% )were significantly higher than those in male and female Non-CAD patients (6.5%)(P<0.05) respectively. 2.A significantly higher percentage of CAD patients had SUA concentrations in the range corresponding to quartile 3(86.6%) and 4(88.1%) than quartile 1(65.3%) and 2(68.9%)(P<0.05).3.Logistic regression analysis showed that HUA was an independent risk factor of CAD (OR=2.639, 95%CI: 1.080～6.443,P=0.033). 4.Significant Spearman’s correlations were found between the SUA level and Gensini score (r =0.270,P < 0.01).The same results were found in different genders( men: r =0.171, P<0.01;women :r =0.298, P<0.01). Conclusion 1. HUA could be an independent risk factor of coronary artery disease. 2. SUA level was correlated with the severity of CAD in patients with angiographic CAD. The relationship trends to be a little stronger in women than that in men. Related Articles | Metrics

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To Discuss the Influence of Different Variety of Mouse on the Model of Acute Pancreatitis association Lung Injury 2010, 38 (5):  0-0 .  Abstract ( 1338 )   PDF (1322KB) ( 1923 )   To discuss the influence of different variety of mouse on the Model of Acute Pancreatitis association Lung Injury.Methods：The AP model was induced by injecting caerulein into the cavity of mice.Caerulein(50 ug／kg body weight)in 0．9％ NaC1 at hourly intervals for 12h、20h；The control animals received the same volume of 0．9％ NaCl without caerulein（0.2ml）.The animals were killed after the last intraperitoneal injection.The severity was evaluated in terms of amylase level，histological changes.To observate the differences between KM, ICR and BALB / C at 12h, 20h.Results：BALB/C 12h experimental group with acute pancreatitis complicated with acute lung injury model succeed：pathology of pancreatic acinar appeared wide variability，and vacuolization acinar necrosis, a large number of infiltrating inflammatory cells.Interstitial and part of the alveolar space have more neutrophils, atelectasis, the gap widened alveolar. KM, ICR was not successful. 20h experimental group found that BALB / C, Kunming mouse model successful, ICR mice with acute pancreatitis only minor damage. Conclusion Balb / C mice of drug response, and less drug use, saving time and costs for acute necrotizing pancreatitis model. Related Articles | Metrics

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Effect of NGF in protecting rat islet cells from Amylin-induced dysfunction. 2010, 38 (5):  0-0 .  Abstract ( 1166 )   PDF (1372KB) ( 1825 )   Abstract Objective:To observe the influence of nerve growth factors (NGF) on vitality and function of the islet cell by the action of Amylin. Methods:Islet cells under the Amylin were incubated with NGF in different concentration ,and we observed the MTT reduction capacity and the amount of insulin secretion.The latter contains the level of insulin both base and high glucose condition .Results: Remarkable decrease both in the secretion of insulin and the reduction capacity on MTT was noticed in the 10umol/lAmylin group. And when were incubated with NGF(>50ng/ml) the inhibition can be dose-dependent relieved. Conclusion: NGF can restore Islet cell’s viability inhibited by high concentration amylin,and recovery the secretory functional of islet B cell. Related Articles | Metrics

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The Assessment of left ventricular twist and untwist in patients with acute infarcted myocardial by two-dimensional speckle tracking 2010, 38 (5):  0-0 .  Abstract ( 1701 )   PDF (1597KB) ( 1965 )   Abstract Objective: To assess the left ventricular twist and untwist in patients with acute infarcted myocardial ( AMI ) by two-dimensional speckle tracking imaging ( STI) .Method: Two-dimensional images of left ventricule (LV) at basal and apical short-axis views were acquired in 60 patients with AMI and 30 healthy subjects to evaluate LV rotation1．Patients with AMI were divided into two groups according to global LV systolic function: normal LV ejection fraction (LVEF) group, (LVEF≥50% , n=36) and abnormal LVEF gruop (LVEF <50% , n = 24 ) .Rotation were measured in the left ventricular short-axis views using 2D-strain software. LV twist were defined as rate of apical LV rotation (PAR)to the basal Peak rotation (PBR) and the time to Prot in basal and apical short axis views were measured separately, Peak twist(Ptw), twist at aortic valve closure(AVCtw), twist at mitral valve opening (MVO tw), untwisting rate(Untw R), and half time of untwisting (HTU )were calculated. Results: Compared with the control group , the value of Ptw、PAR、PBR、AVCtw and MVOtw significantly decreased in abnormal LVEF group；the value of Ptw、PAR、AVCtw and MVOtw decreased in normal LVEF group . Compared with normal LVEF group ,the value of Ptw、AVCtw and MVOtw significantly decreased in abnormal LVEF group.Significant correlation was found between LV twist and LVEF. Conclusion: Left ventricular twist and untwist can be measured using speckle tracking echocardiography. Apical rotation and global twist decrease significantly in patients with myocardial infarction. Related Articles | Metrics

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The studies on the method for the quantitation of pregnancy-associated plasma protein A 2010, 38 (5):  0-0 .  Abstract ( 1198 )   PDF (1006KB) ( 1950 )   Abstract Objective: To develop an Enzyme immunoassay for testing the concentration of pregnancy-associated plasma protein A, the indirect-coating plates were adopted. Methods: The plates were coated with biotinylation bovin serum albumin and avidin-enzyme-linked. A biotin-labeled antibody for PAPP-A was used as catching antibody to capture the antigen of PAPP-A in the sample. The another antibody for PAPP-A that labeled with horseradish peroxidase was used as detecting antibody to test the concentration of pregnancy-associated plasma protein A in human serum. Results: The sensitivity of the assay we developed was 0.31μg/ml and its recovery rates all between 95% and 100%.The linearity, stability and specificity of the assay are good. The three serum samples that the concentration of PAPP-A each at low, middle and high levels were tested repeatedly 10 times, showing that the CVs inner the group all did not exceed 10% and the CVs between groups all did not exceed 15%.The assay we developed was done the analysis associativity with enzyme immunoassay kit abroad, r=0.982, which indicated the two assays were correlated greatly. Conclusion: Using indirect-coating technology, the assay we established to determine PAPP-A is sensitive and accurate, and the stability is good. There are greatly correlated between the assays, we developed and abroad. Related Articles | Metrics