Tianjin Medical Journal

The effects of down-regulation of miR-106a-5p on hydrogen peroxide-induced cardiomyocyte damage and JAK1/STAT3 pathway

JIANG Hua, SHEN Yanmei, MA Xunkai

2023, 51 (2): 113-117. doi: 10.11958/20220744

Abstract ( 454 )

HTML ( 7 )

PDF (823KB) ( 1128 )

Objective To investigate the effects of down-regulation of miR-106a-5p on hydrogen peroxide (H₂O₂)-induced cardiomyocyte damage and Janus kinase 1 (JAK1)/signal transducer and activator of transcription 3 (STAT3) pathway. Methods Different concentration gradients of H₂O₂ (0, 50, 100, 200 and 400 μmol/L H₂O₂) were used to treat rat cardiomyocytes H9c2. Rat cardiomyocytes H9c2 were divided into the control group, the H₂O₂ group (100 μmol/L H₂O₂), the miR-106a-5p NC group (100 μmol/L H₂O₂+ transfected with miR-106a-5p NC) and the miR-106a-5p siRNA group (100 μmol/L H₂O₂+ transfected with miR-106a-5p siRNA). Real-time fluorescent quantitative PCR (qPCR) method was used to detect the expression of miR-106a-5p. CCK-8 method was used to detect cell proliferation. Flow cytometry was used to detect cell apoptosis. LDH content, GSH-Px activity in cell culture fluid, MDA content and SOD activity in cells were detected. Western blotting (WB) was used to detect the expression of JAK1/STAT3 pathway related proteins in cells. Results According to the H₂O₂ concentration gradient experiment, 100 μmol/L H₂O₂ was selected for subsequent experiments. Compared with the control group, the proliferation inhibition rate, apoptosis rate, miR-106a-5p level, MDA content, p-JAK1/JAK1, p-STAT3/STAT3 protein levels in H9c2 cells and LDH content in cell culture fluid were significantly increased in the H₂O₂ group (P<0.05). The GSH-Px activity in cell culture fluid and SOD activity in cells significantly reduced (P<0.05). There were no significant differences in above indicators between the H₂O₂ group and the miR-106a-5p NC group (P>0.05). Compared with the miR-106a-5p NC group, the proliferation inhibition rate, apoptosis rate, miR-106a-5p level, MDA content, p-JAK1/JAK1, p-STAT3/STAT3 protein levels in H9c2 cells and LDH content in cell culture fluid were reduced significantly in the miR-106a-5p siRNA group (P<0.05), and the GSH-Px activity in cell culture fluid and SOD activity in cells increased significantly (P<0.05). Conclusion Down-regulation of miR-106a-5p can inhibit the activation of JAK1/STAT3 pathway, increase the level of anti-oxidative stress and reduce H₂O₂-induced cardiomyocyte damage.

Figures and Tables | References | Related Articles | Metrics

Effects of lupeol on the biological behavior of non-small cell lung cancer NCI-H1299 cells by regulating miR-100-5p

HUANG Yugang, TAN Lingjuan, CHEN Guang

2023, 51 (2): 118-123. doi: 10.11958/20220899

Abstract ( 507 )

HTML ( 6 )

PDF (1246KB) ( 1104 )

Objective To investigate the effect of lupeol on the proliferation, migration and invasion of non-small cell lung cancer (NSCLC) NCI-H1299 cells by regulating miR-100-5p. Methods NCI-H1299 cells cultured in vitro were divided into the normal control (NC) group (untreated), the low-dose group (12.5 μmol/L lupeol), the medium-dose group (25 μmol/L lupeol), the high-dose group (50 μmol/L lupeol), the miR-NC group (transfected with miR-NC), the miR-100-5p group (transfected with miR-100-5p), the anti-miR-NC+high-dose group (the cells were treated with 50 μmol/L lupeol after transfected with anti-miR-NC), and the anti-miR-100-5p+high-dose group (the cells were treated with 50 μmol/L lupeol after transfected with anti-miR-100-5p). CCK-8 and colony formation assays were performed to evaluate the proliferation of NCI-H1299 cells. Transwell method was applied to detect the migration and invasion of NCI-H1299 cells. RT-qPCR was used to detect the expression of miR-100-5p. Western blot assay was used to detect the expression of CyclinD1, matrix metalloproteinase 2 (MMP2) and matrix metalloproteinase 9 (MMP9) proteins. Results Compared with the NC group, NCI-H1299 cell viability, colony formation number, migration number, invasion number, the expression levels of CyclinD1, MMP2 and MMP9 proteins were decreased in the low, medium and high dose groups (P<0.05), and the expression of miR-100-5p was significantly increased (P<0.05). Compared with the miR-NC group, the expression of miR-100-5p was increased, the NCI-H1299 cell viability, colony formation number, migration number, invasion number, the expression of CyclinD1, MMP2 and MMP9 proteins decreased in the miR-100-5p group (P<0.05). Compared with the anti-miR-NC+ high-dose group, the expression of miR-100-5p was decreased, NCI-H1299 cell viability, colony formation number, migration number, invasion number, the expression of CyclinD1, MMP2 and MMP9 proteins increased in the anti-miR-100-5p+ high-dose group (P<0.05). Conclusion Lupeol inhibits the proliferation, migration and invasion of NSCLC cells NCI-H1299 by up-regulating miR-100-5p.

Figures and Tables | References | Related Articles | Metrics

The mechanism of m6A recognition protein HuR inhibiting the growth of colorectal cancer by regulating lncRNA TRG-AS1

CHAI Xiaobing, ZHANG Li, CHU Feifei, WU Huili

2023, 51 (2): 124-130. doi: 10.11958/20220960

Abstract ( 424 )

HTML ( 7 )

PDF (2499KB) ( 1138 )

Objective To investigate the influence of N6-methyladenosine (m6A) recognition protein human antigen R (HuR) on the growth of colorectal cancer (CRC) by regulating long non-coding RNA T cell receptor gamma locus antisense RNA 1 (lncRNA TRG-AS1). Methods The content of m6A in cancer tissue, paracancer tissue and normal colon epithelial cells NCM460 and CRC cells HCT116, SW480 and LOVO were detected by colorimetric method. The expression of TRG-AS1 was detected by real-time fluorescence quantitative PCR (qPCR). The expression of HuR protein was detected by Western blot assay. HCT116 cells were divided into the Ct group, the OE-NC group, the OE-HuR group, the si-NC group, the si-HuR group, the si-HuR + pcDNA group and the si-HuR+ pcDNA-TRG-AS1 group. CCK-8 assay was applied to detect cell proliferation. Plate cloning assay was used to detect the ability of cells to form clones. Flow cytometry was applied to detect apoptosis. Scratch-healing assay was applied to detect cell migration. Transwell assay was used to detect cell invasion. In vivo tumor xenograft experiment was used to observe tumor growth in nude mice. Methylated RNA immunoprecipitation (MeRIP) was applied to detect the presence of m6A sites on TRG-AS1. RNA co-immunoprecipitation (RIP) and RNA pull-down experiments were used to demonstrate the interaction of TRG-AS1 with HuR protein. Results In CRC tissue and cells, HuR protein and TRG-AS1 were highly expressed, and m6A content was decreased. In HCT116 cells, the expression levels of HuR protein and TRG-AS1 were the highest, and the m6A content was the lowest (P<0.05), therefore, HCT116 cells were selected as the research object. Compared with the si-NC group, the expression levels of HuR protein and TRG-AS1 decreased in the si-HuR group, and the m6A content increased (P<0.05). Compared with the OE-NC group, expression levels of HuR protein and TRG-AS1 increased in the OE-HuR group, and the m6A content decreased (P<0.05). Compared with the si-HuR group and the si-HuR+pcDNA group, there were no significant differences in changes of HuR protein and m6A content in the si-HuR+pcDNA-TRG-AS1 group, but the expression of TRG-AS1 increased (P<0.05). The down-regulation of HuR could inhibit HCT116 cell proliferation, migration, invasion and growth of transplanted tumor in vivo, and promote cell apoptosis, while up-regulation of HuR showed the opposite trends. The overexpression of TRG-AS1 attenuated the inhibitory effect of silencing HuR on HCT116 cell proliferation, migration, invasion, in vivo xenograft growth, and the promotion of apoptosis. There was m6A site on TRG-AS1, and TRG-AS1 could interact with HuR protein. Conclusion Silencing the m6A recognition protein HuR can inhibit the proliferation, migration, invasion of HCT116 cells and promote cell apoptosis by inhibiting the expression of TRG-AS1.

Figures and Tables | References | Related Articles | Metrics

Effects of lncRNA OIP5-AS1 regulating miR-25-3p/SOX4 axis on the biological process of human renal tubular epithelial cells induced by high glucose

YANG Juan, ZHANG Houfen, WU Song, CHEN Ying, LUO Huarong

2023, 51 (2): 131-138. doi: 10.11958/20220849

Abstract ( 434 )

HTML ( 5 )

PDF (1527KB) ( 1107 )

Objective To investigate the impact and molecular mechanism of long non-coding RNA Opa-interacting protein 5-antisense transcript 1 (lncRNA OIP5-AS1) on the proliferation, apoptosis and oxidative stress damage of human renal tubular epithelial cells induced by high glucose. Methods Human renal cortical proximal tubule epithelial cells HK-2 were cultured in vitro. HK-2 cells were transfected with lncRNA OIP5-AS1 small interfering RNA (si-OIP5-AS1), miR-25-3p mimic, miR-25-3p inhibitor and their negative controls si-NC, miR-NC and inhibitor-NC. Cells were divided into the normal glucose group (NG group), the high glucose group (HG group), the HG+si-NC group, the HG+si-OIP5-AS1 group, the HG+miR-NC group, the HG+miR-25-3p group, the HG+si-OIP5-AS1+inhibitor-NC group and the HG+si-OIP5-AS1+miR-25-3p inhibitor group. Forty-eight hours after transfection, real-time quantitative PCR (qPCR) was performed to detect levels of lncRNA OIP5-AS1, miR-25-3p and sex-determining region Y-box protein 4 (SOX4) mRNA in cells. CCK-8 assay was performed to detect cell viability and lactate dehydrogenase (LDH) activity in the cell culture supernatant. Flow cytometry was performed to analyze apoptosis, malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) activities in cells. DCFH-DA fluorescent probe was implemented to detect intracellular reactive oxygen species (ROS) production. Western blot experiment was performed to detect the protein expression of SOX4, B-cell lymphoma factor 2 (Bcl-2), Bcl-2-associated X protein (Bax), caspase-3 (Caspase-3) and cleaved-Caspase-3 (Cleaved-Caspase-3) in cells. Dual luciferase reporter assay confirmed the targeting relationship between lncRNA OIP5-AS1 and miR-25-3p, and between miR-25-3p and SOX4. Results Compared with the NG group, the expression levels of lncRNA OIP5-AS1 and SOX4 were significantly increased in the HG group, and the level of miR-25-3p was significantly decreased (P<0.05). Knockdown of lncRNA OIP5-AS1 was able to significantly down-regulate SOX mRNA and protein levels, and up-regulate miR-25-3p level, increase HK-2 cell viability, SOD, CAT activities and Bcl-2 protein level, and reduce apoptosis rate, LDH activity, MDA, ROS levels, Bax protein level and Cleaved-Caspase-3/Caspase-3 ratio (P<0.05). The up-regulating miR-25-3p expression was consistent with that of knocking down lncRNA OIP5-AS1. On the basis of knockdown of lncRNA OIP5-AS1, down-regulation of miR-25-3p significantly attenuated the protective effect of lncRNA OIP5-AS1 knockdown on high glucose-induced oxidative stress damage in HK-2 cells (P<0.05). Dual luciferase reporter assay showed binding sites between lncRNA OIP5-AS1 and miR-25-3p, as well as between miR-25-3p and SOX4. Conclusion The lncRNA OIP5-AS1 may promote high glucose-induced HK-2 cell damage through the miR-25-3p/SOX4 axis.

Figures and Tables | References | Related Articles | Metrics

Effects of tripterygium glycosides on high glucose induced apoptosis of human renal tubular epithelial cells and CXCL10/CXCR3 axis

LI Mingxia, QIAO Haixia, WANG Xiaoling, JIA Liyuan, HU Limei, REN Weidong

2023, 51 (2): 139-143. doi: 10.11958/20221078

Abstract ( 426 )

HTML ( 3 )

PDF (1157KB) ( 1085 )

Objective To investigate effects of tripterygium glycosides (TG) on the apoptosis of human renal tubular epithelial cells HK-2 induced by high glucose and the CXC chemokine ligand 10 (CXCL10)/CXC chemokine receptor 3 (CXCR3) axis. Methods Human renal tubular epithelial cells HK-2 were cultured in vitro and divided into the control group (culture medium containing 5.5 mmol/L glucose), the high glucose group (culture medium containing 25 mmol/L glucose) and the 12.5, 25 and 50 mg/L TG groups (culture medium containing 12.5, 25 and 50 mg/L TG and 25 mmol/L glucose, respectively). Methyl thiazolyl tetrazolium (MTT) method was used to detect the viability of HK-2 cells. Flow cytometry was used to detect the apoptosis of HK-2 cells. 2',7'-dichlorodi-hydrofluorescein diacetate method was used to detect reactive oxygen species (ROS) level of HK-2 cells. Xanthine oxidase method was used to detect superoxide dismutase (SOD) level of HK-2 cells. Enzyme-linked immunosorbent assay was used to detect levels of inflammatory factors of tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) in HK-2 cells. Western blot assay was used to detect the expression of apoptotic proteins (Caspase-3, Caspase-9), CXCL10 and CXCR3 proteins in HK-2 cells. Results Compared with the control group, the HK-2 cell viability and SOD level were significantly reduced in the high glucose group, and the apoptosis rate, ROS, TNF-α, TGF-β1 levels and Caspase-3, Caspase-9, CXCL10, CXCR3 protein expression levels were significantly increased (P<0.05). Compared with the high glucose group, the HK-2 cell viability and SOD level were significantly increased in the 12.5, 25 and 50 mg/L TG groups, and the apoptosis rate, ROS, TNF-α, TGF-β1 levels and Caspase-3, Caspase-9, CXCL10, CXCR3 protein expression levels were significantly reduced, and high dose TG has better effect (P<0.05). Conclusion TG can inhibit high glucose induced apoptosis, oxidative stress and inflammation of HK-2 cells, and its mechanism may be related to the inhibition of CXCL10/CXCR3 axis.

Figures and Tables | References | Related Articles | Metrics

Effects of peripheral blood VEGFR-3⁺ monocytes on left ventricular remodeling in mice with hypertension

YANG Guohong, YU Fangfang, CAI Wei, NIU Xiulong, ZHANG Xin, ZHAO Jihong, LI Yuming, CHEN Shaobo

2023, 51 (2): 144-148. doi: 10.11958/20221051

Abstract ( 405 )

HTML ( 2 )

PDF (1071KB) ( 1089 )

Objective To explore the effects of peripheral blood VEGFR-3⁺monocytes (PBMV) on left ventricular (LV) remodeling induced by high salt intake. Methods Six-week-old male C57BL/6 mice were randomly divided into the 0.5% NaCl concentration diet (W/W, normal salt, NS) group, the 8% NaCl concentration diet (W/W, high salt, HS) group, the HS+ N-nitro-L-arginine methyl ester (L-NAME, HS+L) group and the HS+L+PBMV treatment group. PBMV intervention was performed in hypertensive mice from 9-12 weeks after high salt diet intervention. Noninvasive tail pressure was measured by standard tail pressure method in all mice. Cardiac structure and function were determined using a Vevo 2100 ultrasound imaging system. The mRNA levels of tonicity responsive enhancer binding protein (TonEBP) and lymphatic endothelial cell markers in mouse myocardial tissue were detected by fluorescence quantitative PCR. Masson staining was used to observe the degree of myocardial fibrosis. Calculation of cardiomyocyte cross-sectional area (CSA) was analyzed by fluorescence staining of malt germ lectin (WGA). Results At the end of the intervention, the systolic pressure (SBP) was significantly increased in the HS+L group compared with the other groups (P<0.05). The mRNA levels of TonEBP was significantly lower while the lymphatic endothelial cell markers of VEGF-C, Prox-1, Podoplanin and LYVE-1 were significanly higher in the HS+L+PBMV group than those of the HS+L group (P<0.05). Echocardiography measurement demonstrated that the LV end-diastolic dimension (LVEDD) and LV posterior wall thickness (LVPWT) were significantly decreased in the HS+L+PBMV group than those of the HS+L group. The LV ejection fraction (LVEF) and LV fractional shortening (LVFS) were significantly increased in the HS+L+PBMV group compared with those of the HS+L group (P<0.05). The pathological results showed that the CVF and CSA were significantly decreased in the HS+L+PBMV group than those of the HS+L group (P<0.05). Conclusion The present study demonstrates that PBMV treatment can reduce SBP and ameliorate LV remodeling in hypertensive mice, which may be related to promoting lymphangiogenesis.

Figures and Tables | References | Related Articles | Metrics

Effect of electroacupuncture on the activation of microglia in rats with hypoxia-ischemia brain damage by regulating Nrf2/HO-1 pathway

ZHANG Yun, LI Ke, BU Wangzhen

2023, 51 (2): 149-154. doi: 10.11958/20220792

Abstract ( 490 )

HTML ( 1 )

PDF (892KB) ( 1110 )

Objective To explore the effect of electroacupuncture regulating nuclear factor E2-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1) pathway on the activation of microglia in rats with hypoxia-ischemia brain damage (HIBD). Methods Forty SD rats were randomly divided into the sham operation group, the model group, the electroacupuncture group and the electroacupuncture + all-trans retinoic acid group. Rat model of HIBD was established by common carotid artery ligation and hypoxia in the three groups except the sham operation group. In the sham operation group, only the left common carotid artery and vagus nerve were exposed no ligation and hypoxia treatment. After modeling, electroacupuncture stimulation at Baihui point was given to the electroacupuncture group. Rats in the electroacupuncture + all-trans retinoic acid group were given all-trans retinoic acid (7 mg/kg) intraperitoneally after electroacupuncture stimulation. Neurobehavioral scores of rats were evaluated by Longa score. Open field and water maze experiments were used to detect the behavioral changes and cognitive function of rats. TUNEL method was used to detect the apoptosis of nerve cells. Immunohistochemical method was used to detect the expression of microglia marker protein ionized calcium binding adapter molecule 1 (Iba1) in rat brain. Levels of interleukin-10 (IL-10), IL-1β, reactive oxygen species (ROS) and malondialdehyde (MDA) in rat brain were detected with kits. Western blot assay was used to detect the expression levels of CD68, inducible nitric oxide synthase (iNOS), arginase and Nrf2/HO-1 pathway related proteins in brain tissue. Results Compared with the sham operation group, the neurobehavioral score, the escape latency, cerebral cortex nerve cell apoptosis rate, the expression levels of Iba1, IL-1β and MDA, ROS fluorescence intensity, CD68 and iNOS were increased in the model group, and the exercise distance, number of standing times, the number of crossing platform times, levels of IL-10, arginase, Nrf2 and HO-1 decreased (P<0.05). Electroacupuncture can reduce neurobehavioral score, the escape latency, nerve cell apoptosis rate, expression levels of Iba1, IL-1β, MDA, ROS fluorescence intensity, CD68 and iNOS, and increase exercise distance, number of standing times, number of crossing platform times, levels of IL-10, arginase, Nrf2 and HO-1 (P<0.05). The effect of electroacupuncture on the above indicators could be reversed in the electroacupuncture + all-trans retinoic acid group. Conclusion Electroacupuncture may inhibit the hyperactivation of microglia in rats with HIBD by activating the Nrf2/HO-1 pathway, and promote the transition from M1 type to M2 type.

Figures and Tables | References | Related Articles | Metrics

Effects of glycyrrhizin on myocardial HMGB1/TLR4/NF-κB/HIF-1α signaling pathway in chronic kidney disease rats

ZHANG Xue, WANG Jiarui, CHEN Kangyin

2023, 51 (2): 155-159. doi: 10.11958/20220420

Abstract ( 395 )

HTML ( 2 )

PDF (1097KB) ( 1067 )

Objective To investigate the effect of glycyrrhizin (Gly) treatment on high mobility group protein B1/ Toll-like receptor 4/ nuclear factor κB/ hypoxia-inducible factor 1α (HMGB1/TLR4/NF-κB/HIF-1α) signaling pathway on ventricular muscle of chronic kidney disease (CKD) rats. Methods A total of 38 Wistar rats were randomly divided into 4 groups: the sham operation (Sham) group, the Sham+ Gly group, the CKD group and the CKD+ Gly group. CKD model was prepared by nephrectomy in 5/6, and Gly was intraperitoneally injected (80 mg/kg). Four weeks later, hemodynamics and color doppler echocardiography were performed to observe the heart function of rats in each group. Ventricular blood sampling was used to detect biochemical indexes. The expression levels of HMGB1, TLR4, NF-κB and HIF-1α in myocardium were compared. Results Compared with the Sham group, creatinine, uric acid, urea nitrogen and serum magnesium were increased in the CKD group (P<0.05), blood pressure was also increased (P<0.05). Besides, its diastolic ventricular septal thickness, left ventricular end-systolic diameter were increased, left ventricular ejection fraction decreased, E/A peak decreased and pulmonary artery acceleration time was prolonged (P<0.05). HE staining showed hypertrophy of myocardial cells, and Masson staining showed increased degree of myocardial fibrosis (P<0.05). The expression levels of HMGB1, NF-κB and HIF-1α in myocardial tissue were up-regulated (P<0.05). Compared with the CKD group, rats in the CKD+Gly group showed increased ventricular septal thickness, left ventricular ejection fraction and E/A ratio (P<0.05). HE staining showed that the hypertrophy of myocardial cells was improved, and the degree of myocardial fibrosis decreased (P<0.05). The expression levels of HMGB1, NF-κB and HIF-1α in myocardium were down-regulated (P<0.05). Conclusion CKD can affect cardiac function and aggravate myocardial fibrosis in rats. Glycyrrhizin can improve the degree of myocardial fibrosis in rats with CKD, and the mechanism may be related to the inhibition of HMGB1/TLR4/NF-κB/HIF-1α signaling pathway.

Figures and Tables | References | Related Articles | Metrics

Fluoxetine improves depression-like behavior in CUMS rats by regulating TLR4/NF-κB/NLRP3 inflammasome signaling pathway

LYU Xia, HUANG Li, ZHANG Meilin, FAN Junting, MA Zewei, LIU Huan

2023, 51 (2): 160-165. doi: 10.11958/20220674

Abstract ( 561 )

HTML ( 4 )

PDF (1568KB) ( 1117 )

Objective To investigate the effects of fluoxetine on depression-like behavior in chronic unpredictable mild stress (CUMS) model rats based on Toll-like receptor 4 (TLR4) /nuclear factor κB (NF-κB)/NOD-like receptor pyrin domain-associated protein 3 (NLRP3) inflammasome signaling pathway. Methods Eighteen SD rats were randomly divided into the control group, the model group and the fluoxetine group. Rats in the model group and the fluoxetine group were randomly given CUMS for 11 weeks to establish depression model. The fluoxetine group was treated with fluoxetine (10 mg·kg^-1·d^-1) through gastric perfusion from the 7th to the 11th week, and the other groups were given 1 mL of normal saline by gavage. Behavioral tests were conducted after the intervention. The levels of interleukin (IL)-1β and IL-18 in brain tissue were tested by enzyme-linked immunosorbent assay (ELISA). The expression of NLRP3 protein, apoptosis-associated speck-like protein (ASC) and cysteine aspartate protease 1 (Caspase-1) protein in hippocampal CA3 area and cortical area regions were observed by immunofluorescence staining. Western blot assay was used to detect the protein expression levels of TLR4, NF-κB, NLRP3, Caspase-1 and activated cysteine aspartate protease 1 (cleaved Caspase-1) in brain tissue. Results Compared with the model group, the movement distance and standing times of rats in open field test were significantly increased, the movement distance in the elevated plus maze test was increased, and the dwell time in closed arms was decreased in the fluoxetine group. The contents of IL-1β and IL-18 in brain tissue were significantly decreased, and expression levels of TLR4, NF-κB, NLRP3, ASC, Caspase-1 and cleaved Caspase-1 proteins were also down-regulated in the fluoxetine group than those of the model group (P<0.05). Conclusion Fluoxetine may improve depression-like behavior in CUMS rats by inhibiting TLR4/NF-κB/NLRP3 inflammasome signaling pathway and reducing the levels of inflammatory factors IL-1β and IL-18 in brain tissue.

Figures and Tables | References | Related Articles | Metrics

The relationship between changes of the macular ganglion cell-inner plexiform layer (GCIPL) thickness of the affected eye in patients with unilateral wet age-related macular degeneration and serum interleukin-8 level

ZHANG Lijun, LI Xiangwei, TAN Xiaohui

2023, 51 (2): 166-170. doi: 10.11958/20220889

Abstract ( 296 )

HTML ( 4 )

PDF (790KB) ( 1074 )

Objective To investigate changes of macular ganglion cell-inner plexiform layer (GCIPL) thickness of the affected eye in patients with unilateral wet age-related macular degeneration (wetAMD) and its relationship with serum interleukin-8 (IL-8) level. Methods A total of 120 patients with unilateral wetAMD were selected as the wetAMD group. According to the test results of fluorescein fundus angiography and indocyanine green angiography, patients were divided into the unilateral typical choroidal neovascularization (tCNV) group (45 cases) and the unilateral polypoid choroidal vasculopathy (PCV) group (75 cases). Seventy-five peoples (75 eyes) with normal ophthalmic examination in clinic physical check-up were enrolled as the normal control group. The general data were collected in the three groups. The GCIPL thickness parameters of studied eyes and the serum level of IL-8 were also detected. The above indicators were compared between the three groups. The relationship between GCIPL thickness, serum IL-8 level and wetAMD was analyzed, and the diagnostic value of GCIPL thickness was also analyzed. Results The proportion of smoking, proportion of diabetes mellitus and serum IL-8 level were significantly higher in the wetAMD group than those in the control group, while each measured value of GCIPL thicknesses in eyes with wetAMD was significantly lower than those in eyes of normal control group (P<0.05). The age, smoking rate, proportion of diabetes mellitus and serum IL-8 level were significantly higher in the tCNV group than those in the PCV group, while each measured value of GCIPL thicknesses in eyes with tCNV was significantly lower than that in the PCV eyes (P<0.05). Each measured value of GCIPL thicknesses in the affected eyes in patients with wetAMD was negatively correlated with serum IL-8 level (P<0.05). Multivariate Logistic regression analysis showed that smoking, diabetes mellitus, the decreased value of average GCIPL thickness and the elevated serum level of IL-8 were risk factors of wetAMD, and diabetes mellitus, the decreased value of average GCIPL thickness and the elevated serum level of IL-8 were risk factors of tCNV in patients with wetAMD (P<0.05). The area under the receiver operating characteristic curve (AUC) of the average GCIPL thickness in the diagnosis of wetAMD was 0.775 (95%CI: 0.710-0.832). When the cut-off value was 75.60 μm, the sensitivity was 63.33% and the specificity was 81.33%. The AUC of the average GCIPL thickness in the diagnosis of tCNV was 0.765 (95%CI: 0.678-0.837). When the cut-off value was 70.20 μm, the sensitivity was 64.44%, and the specificity was 82.62%. Conclusion The GCIPL thickness of the affected eye in patients with unilateral wetAMD is abnormally decreased, which is related to the increased serum level of IL-8, and the GCIPL thickness has certain auxiliary value for the diagnosis of wetAMD and tCNV.

Figures and Tables | References | Related Articles | Metrics

The effects of insulin injection related lipohypertrophy on short-term glycemic variability in patients with diabetes

ZHOU Huijuan, ZOU Shengyi, WU Jiang, HU Yukun, XU Ying

2023, 51 (2): 171-175. doi: 10.11958/20220995

Abstract ( 335 )

HTML ( 5 )

PDF (875KB) ( 1084 )

Objective To investigate the effects of insulin-mediated lipohypertrophy (LH) on short-term glycemic variability of hospitalized diabetic patients treated with insulin pump. Methods A total of 232 inpatients with diabetes who received both short-term insulin pump therapy and dynamic blood glucose monitor were retrospectively analyzed, including 52 cases of type 1 diabetes mellitus (T1DM) and 180 cases of type 2 diabetes mellitus (T2DM). The incidence of insulin injection-related LH in patients with different types of diabetes was counted, and the differences in short-term glucose fluctuation indicators between patients with LH and those without LH were compared. The relationship between mean glucose fluctuation amplitude (MAGE) and clinical indicators was analysed. Binary Logistic regression was used to analyse the factors influencing glucose fluctuations. Results The overall incidence of LH was 61.2% in 232 patients, with 57.7% (30/52) in T1DM patients and 62.2% (112/180) in T2DM patients. In both T1DM and T2DM patients, MAGE was positively correlated with LH and negatively correlated with BMI, years of insulin use, and duration of diabetes (P < 0.05). Multi-factor Logistic analysis showed that LH was a risk factor for blood glucose fluctuations in both T1DM (OR= 4.665, 95%CI: 1.134-19.200) and T2DM (OR=6.659, 95%CI: 3.248-13.654) patients. Conclusion Insulin-mediated lipohypertrophy is identified as a vital risk factor for short-term glycemic fluctuations in diabetes.

Figures and Tables | References | Related Articles | Metrics

Analysis of macular choroidal thickness in patients with grade 3 essential hypertension

XU Xuemei, LI Chunyan, DENG Zhihong, XU Xiaoming, SHI Jing

2023, 51 (2): 176-179. doi: 10.11958/20220487

Abstract ( 683 )

HTML ( 1 )

PDF (774KB) ( 1060 )

Objective To observe changes of choroidal thickness in patients with primary grade 3 hypertension and analyze its related influencing factors. Methods Twenty eight patients (56 eyes) with primary grade 3 hypertension were selected as the hypertension group, and 29 healthy volunteers (58 eyes) with normal fundus were selected as the normal group. The choroidal thickness in macular region was measured by optical coherence tomography enhanced deep imaging in patients of the two groups. The choroidal thickness in different macular regions was observed in the two groups, and the retinal thickness in each macular region of the two groups was compared. The correlation between choroidal thickness and retinal thickness and course of disease in patients with hypertension was analyzed. Simple linear regression was used to analyze the effect of age on choroidal thickness. Results The choroidal thickness in N6 zone of macula was significantly lower than that in other areas in two groups (P<0.05). The choroidal thickness in central area, S3, T3, I3, S6, T6 and I6 was significantly lower in the hypertensive group than that in the normal group (P<0.05). Compared with the normal group, the retinal thickness in I6 and N6 area was decreased in the hypertensive group (P<0.05). There was a positive correlation between choroidal thickness and retinal thickness in S3 and S6 zones of the hypertension group (P<0.05). There was no correlation between the duration of hypertension and the thickness of choroid in each area of macula (P>0.05). There was no correlation between patient age and thickness of choroid in N3 zone in the hypertension group (P>0.05), but there was weak correlation with the thickness of choroid in central, S3, I6 and N6 zone (adjusted R²<0.100, P<0.05). It was negatively correlated with thickness of choroid in the T3, I3, S6 and T6 zones (adjusted R²: 0.118, 0.114, 0.102 and 0.155, P<0.05). Conclusion The thickness of choroid in macular area is lower in patients with primary grade 3 hypertension than that in normal population. Changes of choroid thickness is correlated with retinal thickness and age, and no correlation with disease duration.

Figures and Tables | References | Related Articles | Metrics

The treatment of giant rotator cuff tear with enhanced reconstruction of the superior capsule and rotator cable by "L" shape transposition of long head of biceps tendon

WANG Ming, LI Haibin, MIAO Jun

2023, 51 (2): 180-185. doi: 10.11958/20221135

Abstract ( 582 )

HTML ( 5 )

PDF (1483KB) ( 1237 )

Objective To investigate the clinical effect of arthroscopic reconstruction of the superior capsule and rotator cable with "L" shape transposition of long head of biceps tendon in the treatment of giant rotator cuff tear. Methods Thirty-six patients with giant rotator cuff tears were included. All patients were treated by shoulder arthroscopy, in which the long head of the biceps tendon was "L" shaped transposed for joint reconstruction of the superior capsule and rotator cable, followed by repairing the remaining rotator cuff tissue. The patient's surgical condition and complications were recorded. The active shoulder mobility, visual analogue score of pain (VAS), American Shoulder and Elbow Surgery (ASES) score, Constant-Murley score and Fudan University Shoulder Function Score (FUSS) were used for evaluating shoulder joint function and pain before and after surgery. Anteroposterior X-ray and three-dimensional CT were used to evaluate the degree of shoulder degeneration and proximal humeral displacement distance (PHM). MRI was used to assess the structural integrity of the reconstructed tissue. Results The operation was successful in all patients. Twenty-one patients (58%) underwent full rotator cuff repair with single- or double-row suture bridge fixation. Fifteen patients (42%) underwent partial rotator cuff repair. Subscapularis tendon tears were repaired using a single-row technique in 25 patients. Two to five anchor nails were used during surgery, with a mean of (3.8±1.4). Fourteen patients underwent limited acromioplasty. Twelve cases underwent humeral megacartilage, and 10 cases underwent both acromioplasty and humeral megacartilage. The operative times ranged from 50 to 110 min, with a mean of (83.21±21.57) min. The puncture openings healed grade A, and no complications such as infection, fat liquefaction or vascular nerve injury were found. At 6 and 12 months after surgery, the active shoulder mobility and shoulder VAS, ASES, Constant-Murley, and FUSS scores were significantly improved at the last follow-up compared with the preoperation. The joint mobility and scores showed continuous improvement with the extension of the follow-up time (P<0.01). 3D CT showed a significant reduction in PHM at the last postoperative follow-up compared with the preoperative period (P<0.01). MRI follow-up evaluation showed that the reconstructed tissue was structurally intact in 34 cases, with a healing rate of 94.4%. Conclusion The treatment of giant rotator cuff tears by combined augmented reconstruction of the long head of biceps tendon with the rotator cable under shoulder arthroscopy can significantly relieve pain and improve shoulder joint function, and the re-tear rate is low, which is worthy of clinical promotion.

Figures and Tables | References | Related Articles | Metrics

Effects of ultrasound-guided right stellate ganglion block on postoperative visceral pain, nausea and vomiting in patients undergoing laparoscopic cholecystectomy

HUANG Xiaoci, CHEN Qi, SHENG Kui, SHAO Yanmei, TANG Xinyu, HU Xianwen

2023, 51 (2): 186-189. doi: 10.11958/20220866

Abstract ( 330 )

HTML ( 5 )

PDF (839KB) ( 1057 )

Objective To investigate the effect of ultrasound-guided right stellate ganglion block (SBG) on postoperative visceral pain and postoperative nausea and vomiting (PONV) in patients undergoing laparoscopic cholecystectomy (LC). Methods A total of 120 patients scheduled for elective LC were divided into the SGB group (n=60) and the control group (n=60) using a random number table. Ultrasound-guided right SGB was conducted with 1% lidocaine 6 mL before surgery in the SGB group, and the equal volume of normal saline was given at the same site in the control group. Local infiltration anesthesia at incision site was performed with 1% ropivacaine 10 mL at the end of surgery in both groups. The mean arterial pressure (MAP) and heart rate (HR) were recorded at different time points, including before anesthesia induction (T₀), after endotracheal intubation (T₁), skin incision (T₂), pneumoperitoneum establishment (T₃), 10 min after pneumoperitoneum establishment (T₄) and at the end of surgery (T₅). Postoperative visceral pain scores evaluated by the Numerical Rating Scale (NRS) at four times (0.5 h, 4 h, 12 h, 24 h after surgery), incidence of PONV and rate of using antiemetics during the first 24 h after surgery were recorded. The operation time, total dose of propofol and remifentanil, extubation time, first anal exhaust time, Athens Insomnia Scale (AIS) scores on the first night after surgery and satisfaction scores were recorded as well. Results The MAP and HR were significantly lower at T₁ and T₄ in the SGB group than those in the control group (P<0.05). Compared with the control group, the SGB group showed lower NRS scores of visceral pain at 4 h and 12 h after surgery, lower incidence of PONV and lower rate of using antiemetics (P<0.05). Meanwhile, earlier first anal exhaust, lower AIS scores on the first night after surgery and higher satisfaction scores were observed in the SGB group (P<0.05). Conclusion Ultrasound-guided right SGB can alleviate postoperative visceral pain in patients after LC, reduce the incidence of PONV and improve the quality of sleep after surgery.

Figures and Tables | References | Related Articles | Metrics

Comparative study on clinical characteristics and TyG index in patients with premature preeclampsia and chronic hypertension complicated with preeclampsia

LI Jing, FAN Zhuoran, ZHANG Junnong, SHENG Hongna, HUA Shaofang

2023, 51 (2): 190-193. doi: 10.11958/20220717

Abstract ( 597 )

HTML ( 1 )

PDF (712KB) ( 1082 )

Objective To study the clinical characteristics and perinatal outcomes of patients with premature preeclampsia (PE) and chronic hypertension complicated with PE, and to compare the difference of triglyceride-glucose index (TyG) between the two groups of patients. Methods A total of 158 cases of pregnancy terminated by cesarean section at 28-36⁺⁶ weeks of gestation due to "preeclampsia" were selected. According to whether they had chronic hypertension, patients were divided into the chronic hypertension PE group (53 cases) and the PE group (105 cases). The general data, blood routine, coagulation routine, biochemical indexes and TyG were compared between the two groups, and the difference in perinatal outcomes was analyzed. Results Compared with the PE group, patients in the chronic hypertension PE group had older age, higher gestational weight gain during pregnancy, and the gestational age of delivery is earlier, and the difference between the two groups was statistically significant (P<0.05). Data of hematocrit, platelet, fibrinogen, creatine kinase isoenzyme, aspartate aminotransferase, total cholesterol, triglyceride, fasting blood glucose and 24-hour urinary protein were significantly increased in the chronic hypertension PE group, while uric acid was lower (P<0.05). TyG index was also higher in the chronic hypertension PE group (9.89±0.30 vs. 9.76±0.26, P<0.01). The neonatal birth weight and 1-minute Apgar score were lower in the chronic hypertension PE group, but the arterial blood alkaloid surplus was higher and the length of hospital stay in NICU was longer (P<0.05). For both types of preterm PE, the preterm delivery near term (34-36⁺⁶weeks of gestation) accounted for the largest proportion, but the early preterm birth (28-31⁺⁶weeks of gestation) was higher in the chronic hypertension PE group (28.3% vs. 10.5%, P<0.01), and the proportion of small-gestational age babies (SGA) was also higher (60.4% vs. 42.9%，P<0.05). Conclusion Compared with preterm PE, patients with preterm chronic hypertension PE have more significant insulin resistance, hypercoagulability, myocardial and kidney damage, and higher risk of early preterm delivery and SGA. Maternal and fetal monitoring should be more intensive.

Figures and Tables | References | Related Articles | Metrics

Expression and significance of peripheral blood mononuclear cell DNMT1 and serum IL-6 in diabetic nephropathy

XU Limin, XIE Yan

2023, 51 (2): 194-197. doi: 10.11958/20220812

Abstract ( 442 )

HTML ( 6 )

PDF (770KB) ( 1068 )

Objective To investigate the expression and clinical significance of peripheral blood mononuclear cell DNA methyltransferase 1 (DNMT1) and serum interleukin-6 (IL-6) in diabetic nephropathy (DKD). Methods A total of 150 DKD patients (the DKD group) and 50 healthy subjects (the control group) were selected. Data of IL-6, DNMT1, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), serum creatinine, fasting blood glucose, estimated glomerular filtration rate (eGFR), 24-hour urinary albumin excretion rate (24 h UAER) and other clinical indicators were compared between the two groups. Pearson correlation was used to analyze the correlation between IL-6, DNMT1 and other clinical indicators. Multivariate Logistic regression was used to analyze the influencing factors of DKD. Receiver operating curve (ROC) was used to analyze the diagnostic value of IL-6, DNMT1 and combined detection in DKD. Results Compared with the control group, levels of fasting blood glucose, IL-6, DNMT1, serum creatinine, 24 h UAER, LDL-C and glycosylated hemoglobin were higher in the DKD group, but levels of eGFR and HDL-C were lower (P<0.05). IL-6 was positively correlated with DNMT1 in the DKD group (r=0.560, P<0.05), and IL-6, DNMT1 and 24 h UAER were positively correlated (r=0.551, 0.570, P<0.05). Elevated 24 h UAER, elevated IL-6 and elevated DNMT1 were independent risk factors for DKD (P<0.05). The combined detection of 24 h UAER, IL-6 and DNMT1 in the area under the surface of DKD was significantly higher than that of single index detection. Conclusion DNMT1 and IL-6 are highly expressed in DKD patients, and the combined detection of 24 h UAER, DNMT1 and IL-6 can be used as a good indicator for clinical diagnosis of DKD.

Figures and Tables | References | Related Articles | Metrics

Changes and significance of serum YKL-40 and CCL18 levels in patients with CTD-ILD

WANG Dongmei, YUAN Baojun, GAO Lichang, YUAN Wei

2023, 51 (2): 198-202. doi: 10.11958/20220567

Abstract ( 411 )

HTML ( 2 )

PDF (763KB) ( 1146 )

Objective To explore the expression and clinical significance of serum levels of YKL-40 and CCL18 in patients with connective tissue disease-associated interstitial lung disease (CTD-ILD). Methods A total of 105 CTD patients including 37 patients with ILD (the CTD-ILD group), 68 patients without ILD (the CTD group) were enrolled into the study. Thirty healthy people were selected as the control group. Clinical data and the test of high resolution CT (HRCT) were detected in the three groups. The serum levels of YKL-40 and CCL18 were detected by ELISA in the three groups. The correlation between lung HRCT score and YKL-40, CCL18 were analyzed. Results The serum levels of YKL-40, CCL18 and CEA were significantly higher in the CTD-ILD group than those in the CTD group. Comparing with the control group, the serum level of YKL-40 was significantly higher in the CTD-ILD group and the CTD group, and the serum level of CCL18 was significantly higher in the CTD-ILD group (P<0.05). Multivariate Logistic regression analysis showed that higher YKL-40 and CCL18 levels were independent risk factors of ILD in CTD patients (P<0.05). ROC curve analysis showed that areas under the curve (AUC) of serum YKL-40 and CCL18 in the diagnosis of CTD-ILD were 0.648 and 0.621, respectively, while AUC of combined diagnosis of CTD-ILD were 0.663 and their sensitivity were higher than those of YKL-40 and CCL18 alone. Correlation analysis showed that levels of YKL-40 and CCL18 were positively correlated with HRCT scores of the CTD-ILD group (respectively r=0.697 and 0.472, both P<0.05). Conclusion The high serum YKL-40 and CCL18 are highly expressed in CTD-ILD patients, and which are independent risk factors of ILD. The combined detection of serum YKL-40 and CCL18 is helpful for the early diagnosis and severity evaluation of CTD-ILD.

Figures and Tables | References | Related Articles | Metrics

Donafenib combined with HAIC and sintilimab for hepatocellular carcinoma with tumor thrombus in inferior vena cava and achieved complete remission: a case report

ZHANG Wei, GAO Wei, LIU Chen, LIU Xiaomeng, ZHANG Qin

2023, 51 (2): 203-206. doi: 10.11958/20221916

Abstract ( 740 )

HTML ( 1 )

PDF (1397KB) ( 1177 )

Objective To investigate the effect of hepatic artery infusion chemotherapy combined with targeted and immunotherapy on hepatocellular carcinoma (HCC) with inferior vena cava tumor thrombus (IVCTT). Methods A case of hepatocellular carcinoma with tumor thrombus in the inferior vena cava (IVC) near the right atrium was treated with donafenib combined with hepatic artery infusion chemotherapy (HAIC) and sintilimab. The response of tumor treatment was evaluated by RECEST v1.1. The dynamic changes of alpha-fetoprotein (AFP) and PIVKA-Ⅱ were observed and the surgical procedure was introduced. Results After four cycles of treatment with donafenib combined with HAIC and sintilimab, the diameter of the tumor and the tumor thrombus in IVC were successfully reduced, and AFP was reduced to normal. Both tumor and tumor thrombus were completely removed by total hepatic vascular exclusion (THVE) with the IVC incision. Postoperative pathology showed complete remission of the tumor. Conclusion Donafenib combined with HAIC and sintilimab has a good tumor shrinking effect for HCC with IVCTT, and can be applied for conversion surgery for patients of HCC with IVCTT.

Figures and Tables | References | Related Articles | Metrics

The risk factor analysis for immune-related adverse events in non-small cell lung cancer patients treated with immune checkpoint inhibitors and their relationship to effectiveness

XU Jinhui, XU Guangjuan, DUAN Lufen, LIU Kaili, ZHOU Qin, SUN Jiantong, DING Xinyuan

2023, 51 (2): 207-212. doi: 10.11958/20221393

Abstract ( 334 )

HTML ( 3 )

PDF (848KB) ( 1180 )

Objective To explore risk factors for immune-related adverse events (irAEs) in patients with non-small cell lung cancer (NSCLC) treated with immune checkpoint inhibitors (ICIs) and to investigate the relationship between irAEs and effectiveness. Methods A total of 162 NSCLC patients treated with ICIs were retrospectively enrolled. Baseline demographic and clinical characteristics, peripheral blood biomarkers and irAEs were collected and evaluated. Logistic regression analysis was performed to identify risk factors of irAEs. The relationships between irAEs, overall response rate (ORR), progression-free survival (PFS) and overall survival (OS) were also evaluated. Results Eighty-three patients experienced 130 times of irAEs and 12 patients had severe irAEs. The most observed irAEs were skin toxicity (34.6%), immune-related pneumonitis (21.5%), and the most common severe irAEs was immune-related pneumonitis (5.4%). A higher pretreatment absolute lymphocyte count (ALC) was an independent risk factor for irAEs in all patients (OR=3.195, 95%CI: 1.341-7.610, P=0.009). They were also independent risk factors for irAEs in patients treated with ICIs only (OR=5.613, 95%CI: 1.109-28.411, P=0.037) and in patients treated with chemoimmunotherapy (OR=2.901, 95%CI: 1.102-7.633, P=0.031). Receiver operating characteristic curve analyses revealed that the ALC level yielded an AUC of 0.713 (95%CI: 0.633-0.792) with 71.1% sensitivity and 62.0% specificity at the cut-off level of 1.25×10⁹/L to predict the risk of irAEs. Compared with the non-irAEs group，the objective response rate (ORR) was higher in the irAEs group (38.6% vs. 21.5%, χ²=5.567, P<0.05). Patients in the irAEs group had a longer PFS (329 d vs. 206 d, Log-rank χ²= 5.256, P<0.05) as well as OS (690 d vs. 434 d；Log-rank χ²= 4.670, P<0.05). Conclusion An increased baseline ALC level is identified as an independent risk factor for the development of irAEs in NSCLC patients. Moreover, the occurrence of irAEs is significantly associated with the improvement of ORR, PFS and OS.

Figures and Tables | References | Related Articles | Metrics

Clinical value of delayed thoracic drainage in the treatment of blunt traumatic medium volume hemothorax

LI Shuman, LI Jianguo, LIU Lizhi, LI Yanxin, LI Quan

2023, 51 (2): 213-215. doi: 10.11958/20221150

Abstract ( 286 )

HTML ( 1 )

PDF (690KB) ( 1049 )

Objective To explore the therapeutic and prognosis effects of delayed thoracic drainage in the treatment of medium volume hemothorax caused by blunt trauma. Methods Forty-two patients with medium volumn of blunt trauma hemothorax were selected as the delayed group. Clinical observation was carried out first, and catheterization was performed 72 hours after diagnosis. Another 43 patients with blunt trauma medium hemothorax were treated with traditional early catheterization within 24 hours after diagnosis, and they were used as the control group. Data of gender, age, body mass index, chest volume on admission, number of rib fractures, hemoglobin (Hb), systolic blood pressure, mean arterial pressure, heart rate and the interval from injury to admission were compared between the two groups. Data of final thoracic drainage volume, oxygen saturation (SpO₂) at discharge, decline level of Hb, number of days with tube, length of hospital stay, total hospitalization cost, presence of thoracic infection and atelectasis, and converted to surgery were also compared between the two groups. Results There were no significant differences in basic conditions between the two groups. Compared with the control group, the final thoracic drainage volume and Hb decreased, the days with tube, the days of hospitalization and the total hospitalization cost decreased, and SpO₂ increased after hospital discharge in the delayed group (P<0.01). No chest infection or atelectasis occurred in the delayed group. There were no cases requiring transfer to surgery in the two groups. Conclusion Delayed thoracic drainage can effectively improve the treatment and prognosis of patients with blunt traumatic medium volumn hemothorax, and reduce the medical economic burden.

Figures and Tables | References | Related Articles | Metrics

Detection and clinical significance of serum GGT and CTHRC1 expression levels in patients with Alzheimer's disease

CHEN Jun, ZHENG Jinhao, CHEN Jialiang, CHEN Bo

2023, 51 (2): 216-220. doi: 10.11958/20221105

Abstract ( 450 )

HTML ( 4 )

PDF (742KB) ( 1108 )

Objective To study the serum levels of gamma-glutamyl transferase (GGT) and collagen triple helix repeat containing 1 (CTHRC1) in Alzheimer's disease (AD) patients and their clinical significance. Methods A total of 120 AD patients diagnosed and treated in our hospital from January 2020 to January 2022 were selected as the AD group, and 60 healthy people who underwent physical examinations during the same period were selected as the control group. Enzyme-linked immunosorbent assay was used to detect serum expression levels of GGT, CTHRC1, tumor necrosis factor α (TNF-α), interleukin (IL)-1β and IL-6 in each group. Serum hypersensitive C-reactive protein (hs-CRP) was detected by immunoturbidimetry. Pearson correlation analysis was used to analyze the correlation between serum GGT, CTHRC1, TNF-α, IL-1β, IL-6, CRP and Concise Mental State Scale (MMSE) score in the AD group. Multivariate Logistic regression analysis was used to analyze risk factors affecting the occurrence of AD. Receiver operating curve analysis was used to analyze the diagnostic value of serum GGT and CTHRC1 levels in the diagnosis of AD. Results The serum levels of GGT, CTHRC1, TNF-α, IL-6, IL-1β and hs-CRP were significantly higher in the AD group than those in the control group, and the MMSE score was significantly lower than that in the control group (P<0.05). The expression levels of serum GGT and CTHRC1 were positively correlated with levels of TNF-α, IL-6, IL-1β and hs-CRP in the AD group, and were negatively correlated with the MMSE score (P<0.05). Increased GGT and CTHRC1 levels and decreased MMSE score were independent risk factors for the occurrence of AD (P<0.05). The areas under the curve (95%CI) of serum GGT and CTHRC1 alone and in combination for the diagnosis of AD were 0.780 (0.726-0.836), 0.728 (0.705-0.810) and 0.909 (0.875-0.942), respectively. The diagnostic value of serum GGT and CTHRC1 combined detection for AD was significantly higher than that of serum GGT and CTHRC1 single index detection (Z=4.370, 5.657, P<0.05). Conclusion The serum expression levels of GGT and CTHRC1 are related to the inflammatory response and cognitive level in AD patients. The combined detection of both has high diagnostic value for AD.

Figures and Tables | References | Related Articles | Metrics

Research progress of long non-coding RNA regulating metastasis of gastric cancer

WANG Lin, WEN Kunming, HUANG Shengkai, SUN Yuemin

2023, 51 (2): 221-224. doi: 10.11958/20221140

Abstract ( 390 )

HTML ( 8 )

PDF (712KB) ( 1067 )

Gastric cancer is a common cancer of the digestive system with a high mortality rate. Distant metastasis of tumor cells is one of main causes of treatment failure and patient death. Finding therapeutic targets for gastric cancer metastasis is of great significance for improving the survival rate of patients. A large number of recent studies have shown that long non-coding RNAs (LncRNAs) are closely related to the metastasis of gastric cancer, and they are involved in various biological processes of gastric cancer metastasis. Therefore, this article reviews the research progress in the regulation of gastric cancer metastasis by LncRNA, in order to provide a theoretical basis for understanding the mechanism of gastric cancer metastasis.

References | Related Articles | Metrics

Table of Content