Abstract: Objective To investigate the expression levels of tumor necrosis factor-α induced protein 8 like-2 (TIPE2) and tissue factor (TF) mRNA in peripheral blood mononuclear cells (PBMCs) and changes of plasma levels of tumor necrosis factor-α (TNF-α) and TF in patients with type 2 diabetes mellitus (T2DM), and to explore the correlation between them and vascular complications. Methods A total of 78 patients diagnosed as T2DM and admitted to Panyu district, the 2nd Affiliated Hospital of Guangzhou Medical University from May 2015 to May 2016 were included in this study. Patients were divided into two groups according to whether they were combined with vascular complications: 42 cases in simple T2DM group and 36 cases in T2DM combined with vascular complication group (T2DM + V). Forty healthy people in healthy physical examination at the same period were enrolled in this study as the control group (CON). Data of age and gender were recorded in three groups. The glycosylated hemoglobin (HbA1c) was detected by high performance liquid chromatography. Fasting peripheral blood samples were collected in early morning, and serum levels of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were detected by automatic biochemical analyzer. The expression of TIPE2 and TF mRNA in PBMCs were detected by RT-PCR, and plasma levels of TF and TNF-α were tested by ELISA. All these indicators were compared, and the correlation of TIPE2 mRNA with TF mRNA, plasma TF and TNF-α were analyzed between the three groups. Results There were no significant differences in age, gender, TC, TG, HDL-C and LDL-C between three groups (P＞0.05). Values of serum HbA1c increased in turn in CON group, T2DM group and T2DM+V group. PBMCs TIPE2 mRNA expressions decreased in turn, and the expressions of TF mRNA increased in turn in CON group, T2DM group and T2DM+V group (P＜0.05). The plasma levels of TF and TNF-α were increased in turn in CON group, T2DM group and T2DM+V group (P＜0.05). TIPE2 mRNA expression were negative correlated with TF mRNA and TNF-α in T2DM+V group (r=-0.340, -0.342, P＜0.05). Conclusion The occurrence and development of T2DM vascular complications are correlated with increased TIPE2 and decreased TF.

Key words: diabetes mellitus, type 2, diabetic angiopathies, tumor necrosis factor-alpha, inflammation, tissue factor, tumor necrosis factor-alpha induced protein 8 like-2