Study on the mechanism of astragalus polysaccharides regulating autophagy of colorectal cancer cells based on PI3K/Akt/mTOR signaling pathway

doi:10.11958/20220463

Abstract

CLC Number:

R285.5

Figures/Tables 12

References 17

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组别	12 h	24 h	48 h
control组	100.00±0.00	100.00±0.00	100.00±0.00
0.25 g/L APS组	96.66±1.46	91.27±1.28^a	75.88±2.26^a
0.5 g/L APS组	88.30±4.85^a	80.26±3.45^ab	62.85±5.85^ab
0.75 g/L APS组	81.41±3.61^ab	57.83±2.89^abc	46.36±1.32^abc
1.0 g/L APS组	69.69±5.05^abcd	51.09±1.51^abcd	39.69±1.96^abc
F	34.740^＊	276.200^＊	195.200^＊

组别	12 h	24 h	48 h
control组	100.00±0.00	100.00±0.00	100.00±0.00
0.25 g/L APS组	96.66±1.46	91.27±1.28^a	75.88±2.26^a
0.5 g/L APS组	88.30±4.85^a	80.26±3.45^ab	62.85±5.85^ab
0.75 g/L APS组	81.41±3.61^ab	57.83±2.89^abc	46.36±1.32^abc
1.0 g/L APS组	69.69±5.05^abcd	51.09±1.51^abcd	39.69±1.96^abc
F	34.740^＊	276.200^＊	195.200^＊

组别	细胞增殖活性	细胞迁移率
control组	100.00±0.00	51.62±1.86
APS组	54.20±3.67^a	35.09±1.18^a
3-MA组	95.15±2.20^b	49.02±1.56^b
APS+3-MA组	70.54±2.36^abc	42.70±1.19^abc
F	231.900^＊	74.950^＊

组别	细胞增殖活性	细胞迁移率
control组	100.00±0.00	51.62±1.86
APS组	54.20±3.67^a	35.09±1.18^a
3-MA组	95.15±2.20^b	49.02±1.56^b
APS+3-MA组	70.54±2.36^abc	42.70±1.19^abc
F	231.900^＊	74.950^＊

组别	LC3BⅡ/Ⅰ	p62
control组	1.00±0.00	1.00±0.00
APS组	1.77±0.11^a	0.61±0.07^a
3-MA组	0.57±0.14^ab	1.49±0.22^ab
APS+3-MA组	1.01±0.13^bc	1.23±0.16^b
F	60.520^＊	21.030^＊

Study on the mechanism of astragalus polysaccharides regulating autophagy of colorectal cancer cells based on PI3K/Akt/mTOR signaling pathway

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组别	p-PI3K/PI3K	p-Akt/Akt	p-mTOR/mTOR
control组	1.00±0.00	1.00±0.00	1.00±0.00
APS组	0.70±0.05^a	0.58±0.23^a	0.71±0.06^a
740Y-P组	1.48±0.19^ab	1.50±0.11^ab	1.24±0.10^ab
APS+740Y-P组	1.16±0.11^bc	1.10±0.15^bc	0.92±0.05^bc
F	25.920^＊	19.360^＊	36.060^＊

组别	p-PI3K/PI3K	p-Akt/Akt	p-mTOR/mTOR
control组	1.00±0.00	1.00±0.00	1.00±0.00
APS组	0.70±0.05^a	0.58±0.23^a	0.71±0.06^a
740Y-P组	1.48±0.19^ab	1.50±0.11^ab	1.24±0.10^ab
APS+740Y-P组	1.16±0.11^bc	1.10±0.15^bc	0.92±0.05^bc
F	25.920^＊	19.360^＊	36.060^＊

组别		LC3BⅡ/Ⅰ		p62		p-PI3K/PI3K
control组		1.00±0.00		1.00±0.00		1.00±0.00
APS L组		1.40±0.06^a		0.75±0.08^a		0.77±0.04^a
APS H组		1.93±0.19^ab		0.52±0.09^ab		0.61±0.05^ab
F		48.580^＊		37.340^＊		73.780^＊
组别	p-Akt/Akt		p-mTOR/mTOR		p62（IOD）
control组	1.00±0.00		1.00±0.00		53 899.86±2 035.99
APS L组	0.76±0.07^a		0.73±0.04^a		36 337.14±1 527.26^a
APS H组	0.58±0.03^ab		0.58±0.02^ab		13 444.72±5 567.52^ab
F	76.990^＊		263.500^＊		98.830^＊

组别		LC3BⅡ/Ⅰ		p62		p-PI3K/PI3K
control组		1.00±0.00		1.00±0.00		1.00±0.00
APS L组		1.40±0.06^a		0.75±0.08^a		0.77±0.04^a
APS H组		1.93±0.19^ab		0.52±0.09^ab		0.61±0.05^ab
F		48.580^＊		37.340^＊		73.780^＊
组别	p-Akt/Akt		p-mTOR/mTOR		p62（IOD）
control组	1.00±0.00		1.00±0.00		53 899.86±2 035.99
APS L组	0.76±0.07^a		0.73±0.04^a		36 337.14±1 527.26^a
APS H组	0.58±0.03^ab		0.58±0.02^ab		13 444.72±5 567.52^ab
F	76.990^＊		263.500^＊		98.830^＊