Abstract: Objective To establish a stable and reliable brain death model in swine, and to provide a more physiological way to investigate brain function, transplantation immunology during brain death. Methods On the basis of classic method of increasing epidural intracranial pressure, Codman intracranial pressure monitoring probes were implanted in landrace pigs invasively, the brain death models were established under real-time monitoring of the intracranial pressure. Results 12 experimental landrace pigs, one died of anesthetic accident, 11 were successfully induced model of brain death, the success rate was 100%. With effective respiratory and circulatory support the brain death models can maintain 31.3 ± 4.7h. Transcranial Doppler, EEG, ECG, mean arterial blood pressure and other monitoring shows that brain death model-making process is stable and reliable. Conclusion This method of inducing brain death model (under ICP monitoring) is stable and easy to be standardized.

Key words: Brain death, Intracranial Pressure, Model, animal