Table of Content

15 October 2014, Volume 42 Issue 10 Previous Issue    Next Issue
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细胞与分子生物学
实验研究
临床研究
流行病学调查
药物临床观察
应用研究
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细胞与分子生物学

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Construction and Application of Lentiviral Vectors Expressing microRNA-203 Tough Decoy 2014, 42 (10):  961-964 .  Abstract ( 645 )   PDF (603KB) ( 2880 )   [Abstract] Objective   To establish method of constructing lentiviral vectors to express microRNA（miRNA） ''tough decoy''（TuD）and to detect the effects of the TuD on cellular endogenous miRNA level and cellular phenotypes. Methods Two-step cloning strategy was utilized to first generate a universal miRNA TuD frame vector，followed by constructing the TuD expression vector specially targeting miR- 203. The package of the recombinant lentivirus was performed in 293T cells. Then the rat bone marrow mesenchymal stem cells（BM-MSCs）were infected by the miR-203 TuD expression lentivirus. The pSIH1-H1-copGFP vector was also packaged and the BM-MSCs infected by this lentivirus were served as control. Endogenous miR-203 level in BM-MSCs was measured by quantitative RT-PCR，and cellular viability and apoptosis were detected by CCK-8 test and Annexin V-PI staining respectively. Results  The miR-203 TuD expression vector was successfully constructed and inserted sequence was validated. At the 3rd，6th and 9th days after in fected by the miR-203 TuD expression lentivirus，rat BM-MSCs exhibited a repressed endogenous miR-203 level. The miR-203 TuD also promoted viability and inhibited apoptosis of BM-MSCs. All these differences between miR-203 TuD group and control group were statistically significant. Conclusion  The two-step cloning method for the construction of miRNA TuD expression vector is simple and efficient. The miRNA TuD can effectively suppress the level of the target miRNA and affect cellular phenotypes. Related Articles | Metrics

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Inhibition Effect of Non Custodial Terpenes -3 β - Alcohol to Autoimmune Encephalomyelitis 2014, 42 (10):  965-968 .  Abstract ( 492 )   PDF (1585KB) ( 2861 )   [Abstract] Objective? To study the inhibition effect of non custodial terpenes -3 β - alcohol to experimentally in? duced autoimmune encephalomyelitis in guinea pigs. Methods? Different doses (25 mg/kg, 50 mg/kg and 100 mg/kg) of non custodial terpenes -3 β - alcohol were given to the experimentally induced autoimmune encephalomyelitis model of guinea pigs by gavage for 8 weeks. Plasma levels of CD4+/CD8+, IL-1, IL-2, IL-6, IL-10, neuropeptide Y (NPY), beta endorphin (β - EP) , transforming growth factor - β (TGF- β), matrix metalloproteinase (MMP-2), nitric oxide synthase (NOS) and leuko? cyte differentiation antigen CD3 were assessed. The brain neuron morphology changes was observed under light microscopy while its ultrastructure changes was observed under electron microscope. NOS expression in neurons was observed through immunofluoresce technology. Results ?Non custodialterpenes -3β- alcohol inhibited the increase of plasma CD4+/CD8+, IL- 1, IL-2, IL-6, IL-10, MMP-2, CD3 and NPY while decrease of plasma β -EP, brain TGF- β. It also increase NOS expres? sion in neuronal cytoplasm and maintained neuron morphology. Conclusion Non custodial terpenes -3 β - alcohol inhibit? ed the experimental autoimmune encephalomyelitis in guinea pig. Related Articles | Metrics

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Effect of PI3K Inhibitor on Proliferation of Human Glomerular Mesangial Cells 2014, 42 (10):  969-971 .  Abstract ( 524 )   PDF (1518KB) ( 2823 )   [Abstract] Objective  To investigate the effect of LY294002 on proliferation of cultured human glomerular mesangial cells, and to study on the mechanism underlying this proliferation. Methods  Different concentrations of LY294002 (0.02，0.2，2，20，100 mg/L) were administrated to the cultured human glomerular mesangial cells. The effects of mesangial cell proliferation were measured using CCK-8 colorimetric assay. Under appropriate concentrations, proliferation of cultured mesangial cells were measured using CCK-8 at 0.5, 1, 24, 48 hours of drug administration time. Results  LY294002（0.02 mg/L）didn’t obviously inhibited the growth of mesangial cells（P＞0.05）, the inhibition rate was 5.05%. The effect of LY294002 on the cells decreased with rising concentration (0.2 to 20 mg/L) in a dose-dependent manner (P<0.05). Mesangial cells would stop growing with the increasing concentration. LY294002 didn’t obviously inhibited the growth of mesangial cells at 2 mg/L during 0 to 1 h（P＞0.05）, but inhibited proliferation gradually from 1 to 48 h (P < 0.05). Conclusion  Within the certain range of concentration and time, the LY294002 inhibits the proliferation of human glomerular mesangial cells, and PI3K/Akt/mTOR signaling pathway may regulate the proliferation of human mesangial cell. Related Articles | Metrics

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Effect of Tumor Stem Cell Derived CSC-DC-CIK on Destructing Homologous Tumor Cells Teng Chong ,Zhang Teng yue,Wang Chang li 2014, 42 (10):  972-976 .  Abstract ( 591 )   PDF (434KB) ( 2881 )   [Abstract] Objective  To investigate the destructive effect of CSC-DC-CIK who were induced by cytokine induced killer (CIK) cells co-cultured with dendritic cells (DCs) on homologous tumor cells and to explore the possibility of CSC anti? gen involving in killing tumor. Methods Kidney cancer stem cells (KSCs) and lung cancer stem cells (LSCs) were isolated through FACS using CD133 + as a selection marker from cultured kidney cancer cell line A498 and lung cancer cell line A549 respectively. Freeze-thaw method was used to obtain the cancer stem cells（CSCs）antigens. DC cells and CIK cells were collected by in vitro expansion and inducted from the mononuclear cells isolated from human cord blood. The CIK cells were co-cultured with the DCs which were pulsed with the CSCs antigens（CSC-DC-CIK）mentioned above. Immunopheno? types of DC and CIK were analyzed by flow cytometry; cytokines levels were detected by ELISA kits and the destructive ef? fects of two kinds of CSC-DC-CIKs were tested by lactate dehydrogenase (LDH) release assay. Results  The expression of phenotypes CD40+, CD80+, CD86+ and HLA-DR+ were higher in CSC-DC than in CD（P < 0.01）; the expression of pheno? types CD40+, CD80+, CD86+ and HLA-DR+ of DC and CSC-DC were higher after co-culture than those before co-culture（ P < 0.01）;the expression of phenotypes CD40+, CD80+, CD86+ and HLA-DR+ of CSC-DC after been co-cultured with CIK were higher than those of DC after been co-cultured with CIK（P < 0.01）. The CIK phenotypes: CD3+, CD8+, CD56+ were in? creased in CIK co-cultured with both CSC-DC and DC than those before co-culture (P < 0.01); the expression of pheno? types CD3+, CD8+, CD56 +were higher in CSC-DC co-cultured with CIK than in DC co-cultured with CIK. DC-CIK and CSC-DC-CIK groups were more capable to express IFN-γ, TNF-α, IL-2 than they were before co-cultured with CIK (P < 0.01). CSC-DC-CIK group can secrete more above cytokines than DC-CIK group does（P < 0.01）. The destructive rates of KSC-DC-CIK and LSC-DC-CIK on target cells were (50.21±4.24)% and (49.32±3.89)% respectively which were much higher than that in DC-CIK（30.25±3.11）%（F = 89.157，P < 0.01）. Conclusion CSC-DC-CIKs have destructive effects on homologous tumor cells. More researches are needed to explore the mechanism and to evaluate the clinical applications. Related Articles | Metrics

实验研究

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Study of Effect of CtBP2 Knockout through shRNA on Proliferation of Prostate Cancer Cells 2014, 42 (10):  977-979 .  Abstract ( 563 )   PDF (370KB) ( 2908 )   [Abstract] Objective  To study the effects of shRNA-CtBP2 on the growth of prostate cancer PC3 cells. Methods There were three experimental groups in this study，which include blank control group, empty plasmid transfected group and transfected shRNA group. CtBP2 mRNA sequence is targeted by 3 pairs of designed interfering shRNA to built shRNA-CtBP2 recombinant plasmid then it is transfected into PC3 cells. Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot assays were used to detect the transcription and expression levels of CtBP2 mRNA and protein, respectively. PC3l proliferation was measured by MTT assay. Results  Builting shRNA-CtBP2 recombinant plasmid and transfect? ing PC3 cells were successful. Transcription and expression levels of CtBP2 mRNA and protein were significantly decreased in shRNA-CtBP2 transfected PC3 cells. After CtBP2 silencing, cell proliferation was blocked in the shRNA-CtBP2 cells compared to that of blank control group (P<0.01). Conclusion  shRNA-CtBP2 could significantly inhibit CtBP2 expres? sion, suppress the growth of PC3 cells, which suggests that CtBP2 may be a new target for PCa gene therapy. Related Articles | Metrics

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Effects of Recombinant Human B-type Natriuretic Peptid on Oxygen Consumption in Acute Myocardial Infarction with Heart Failure 2014, 42 (10):  980-983 .  Abstract ( 537 )   PDF (350KB) ( 2951 )   [Abstract] Objective  To evaluate the impact of different doses of recombinant human B-type natriuretic peptid (rhBNP) within the dosage of clinical rage on oxygen consumption during acute myocardial infarction (AMI) with heart failure (HF). Methods  AMI-HF model of York pig was established by occluding coronary artery with balloon combined with injecting microthrombus. Then animals were randomized into rhBNP group and control group. Clinical dose of rhBNP (1.5 μg kg bolus followed by a continuous infusion with speed of 0.01, 0.02 and 0.03 μg·kg-1·min-1 for 60 minutes respectively in turn) was administrated in rhBNP group while equal volume of saline was given in the control group. Myocardial oxygen uptake (MOU) was measured by drawing blood from coronary artery and coronary sinus using a catheter. Coronary diameter was determined using quantitative coronary angiography. The observation points were at baseline (T0), instant after the model establishment (T1), 60 min after continuous rhBNP infusion of 0.01, 0.02, 0.03 μg·kg-1·min-1 (T2-T4) respectively. Results  Compared with the control group, pulmonary capillary wedge pressure, central venous pressure, heart rate, systolic blood pressure and MOU were significantly decreased after rhBNP administration. And cardiac output and coronary diameter were obviously increased with addition of rhBNP. There is a interaction of drug intervention and time. In rhBNP group, MOU was significantly decreased with drug administraion (T2-T4 vs T1，mL O2/L: 10.61±0.35，9.85±0.60，9.79±0.31 vs 11.59±0.37). Conclusion  Intravenous administration of rhBNP in AMI-HF model could decrease MOU and PCWP while increase the cardiac output. Related Articles | Metrics

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Establishment and Evaluation of Food Induced Model of Metabolic Syndrome in Rabbit MEI Yan 1,WANG Jian ping2,HU Jin fang3 2014, 42 (10):  984-987 .  Abstract ( 551 )   PDF (1029KB) ( 2849 )   [Abstract] Objective? To establish an animal model of metabolic syndrome which is prone to atherosclerosis. Methods? Eight rabbits were randomly selected from in total of 16 rabbits as control group who were fed with normal rabbit feed. And the rest were used as model group, who were fed with high fat diet. Animals were weigh every week, and triacylglycerol (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDLC), and glucose (GLU) content were tested in the 8th, 10th , 13th, 16th and 18th week. Femoral arterial blood pressure and insulin (INS) content were assessed at the end of experimental period which is the 18th week; Also, aortic sclerosis rating, arterial stiffness index, abdominal fat coefficient, liver coefficient and cardiac coefficient were calculated and heart, aorta, liver and lower extremity artery pathology were examed. Results? Compared with the control group, weight in the model group increased significantly; Serum levels of TC, HDL-C, blood pressure abdominal fat coefficient, liver coefficient were significantly increased in model group; the whole blood viscosity, plasma viscosity and blood pressure fat coefficient, liver coefficient, aortic sclerosis rating, arterial stiffness index, and GLU all increased significantly in model group; while HDL-C/TCHO, insulin sensitivity index decreased significantly in model group（P < 0.05 or P＜0.01）. Conclusion Food induced method can form metabolic syndrome and atherosclerosis in rabbit model. Related Articles | Metrics

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Effect of Xuebijing Injection on MIF Expression and Acute Kidney Injury in Rats with Sepsis 2014, 42 (10):  988-991 .  Abstract ( 503 )   PDF (371KB) ( 2894 )   [Abstract] Objective  To investigate the expression of macrophage migration inhibitory factor (MIF) in serum and renal tissue of septic rats with actue kidney injury (AKI), and to explore the effect of Chinese traditional medicine-Xuebijing injection on MIF expression as well as on acute kidney injury in rats with sepsis. Methods  Sepsis model was reproduced in rats with cecal ligation and puncture (CLP)．Eighty healthy SD rats were randomly divided into three groups：sham operation group（n=16）, CLP model group (n=32), and xuebijing group(n=32)．All rats were sacrificed at either 2, or 8, or 24 and or 48 hours after operations．MIF mRNA levels in renal tissues of septic rats were semi-quantified by Real-time PCR．The content of MIF in serum was determined by enzyme linked immunosorbent assay (ELISA). Serum creatinine (Cr) contents were measured by automatic biochemistry analyze. Results  Compared with sham operation group, transcription of MIF mRNA in renal tissues of model group were significantly enhanced at 8, 24 and 48 hours after operations (P < 0.01). Both contents of MIF and creatinine level in serum of model group rose obviously at 24 and 48 hours after operation (P < 0.01)； Compared with model group, the transcription of MIF mRNA in renal tissues of xuebijing group decrease obviously at 2, 8, 24 and 48 hours (P < 0.01) and both contents of MIF and creatinine in serum of xuebijing group drop remarkably at 24 and 48 hours (P < 0.01). Conclusion  MIF is a kind of late cytokine which might participate in the pathogenesis of AKI in rats with sepsis．Xuebijing injection can inhibit MIF expression, and possess the protective effects on the kidney in rats with sep? sis. Related Articles | Metrics

临床研究

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Expression of Mitofusin-2 (mfn2) and nm23 in Different Bladder Tissues BAI yun 2014, 42 (10):  992-994 .  Abstract ( 585 )   PDF (444KB) ( 2849 )   [Abstract] Objective  To research the expression of mitofusin-2 (mfn2) and tumor metastasis suppressor genes （nm23）in bladder cancer cells and its correlation with clinical pathological feature． Methods Immunohistochemistry was used to measure the expression of mfn2 and nm23．Sixty-five cases of bladder cancer were sampled，which include fifty cases of male and fifteen cases of female. TNM stage：Forty-seven cases were in stage I; Ten cases were in stage II; Five cases were in stage Ⅲ; Three cases were in stageⅣ. Other fifteen cases were sampled from normal bladder or benign tumor of bladder as control . All cases were collected from department of pathology，affiliated Hospital of hebei union university. Results  The positive expression rate of mfn2 in bladder cancer tissues was significantly higher than those in normal bladder tissues and benign tumor of bladder（χ2 =32.528，P < 0.05）；The positive expression rate of nm23 in bladder cancer tissues was significantly lower than those in normal bladder tissues and benign tumor of bladder (χ2 =19.719，P<0.05)；the high expression level of mfn2 in bladder cancer was associated with tumor differentiation and TNM stage（P＜0.05），but not correlated with age，sex，lymph node metastasis and clinical grade. The low level of nm23 was associated with TNM stage，clinical grade and LN metastasis. Conclusion  The positive expression rate of mfn2 was increased in bladder cancer. It indicated that there was a close relationship between mfn2 and the occurrence and development of bladder cancer；The expression rate of nm23 was decreased in bladder cancer，it may be a predictor for metastasis and prognosis of bladder cancer. Related Articles | Metrics

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Expression and Clinical Significance of Neuropilin-1 and 2 in Colorectal Carcinoma 2014, 42 (10):  998-1001 .  Abstract ( 525 )   PDF (1527KB) ( 2859 )   [Abstract] Objective  To observe the expression of Neuropilin (NRP)-1, 2, to calculate micro vessel density (MVD) in colorectal cancer, paraneoplastic tissues, normal tissues, and to investigate the correlation between NRP-1, 2 and MVD to understand the role of NRP-1, 2 in the process of tumor angiogenesis. Methods  Expression of NRP-1 and NRP-2 were studied by immunohistochemistry in 66 specimens from colorectal cancer, paraneoplastic tissues and normal tissues. MVD was assessed based on CD105 immunohistochical staining. Results  (1) The positive expression of NRP-1, 2 in colorectal cancer, paraneoplastic tissues, normal tissues were 71.2%, 25.8%, 0; 80.3%, 15.2%, 0 respectively. There was a statistical difference between them (P＜0.05). (2)The quantity of MVD in colorectal cancer, paraneoplastic tissues and normal tissues were 35.682±5.542, 14.485±3.301 and 6.864±1.771 respectively. There was a statistical difference between them (P＜0.05). (3) Expression of NRP-1, 2 had a good correlation with the tumor size, infiltrating depth, lymph node metastasis, Dukes stag? ing (P＜0.05), but no correlation was found with the tumor position, histological types, differentiation degree and the gender or age of patients (P＞0.05). (4) The MVD value had no correlation with the gender or age of patients, tumor size, position, histological types (P＞0.05), but had a good correlation with infiltrating depth, lymphatic metastasis and Dukes staging (P＜ 0.05). (5) There was a positive correlation between NRP-1, 2 and MVD value  (P＜0.01). Conclusion  NRP-1, 2 may play an important role in the angiogenesis of the colorectal cancer, and it correlates with the invasion and metastasis of colorectal cancer closely. Related Articles | Metrics

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Correlation between Endothelial Function with Pulse Pressure Index in Uygur Patients with Acute Coronary Syndrome YANG Yang 2014, 42 (10):  1002-1004 .  Abstract ( 550 )   PDF (283KB) ( 2857 )   [Abstract] Objective  To investigate the correlation of endothelial function variation with pulse pressure index in Uygur acute coronary syndrome patients in Xinjiang. Methods  Uygur patients were divided into acute coronary syndrome group (n=208) and control group (n=157) according to their Coronary angiography results. Height, weight and blood pressure were measured on admission and basic medical history were recorded. Fasting blood glucose (FBG), three acyl glycerin (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL - C), low density lipoprotein cholesterol (LDL - C) were examined using fasting venous blood; Plasma Coagulation Factor Ⅷ (CFⅧ) and von Willebrand factor（vWF）were examined using ELISA. Results  There are no statistical difference in age, gender, history of hypertension, diabetes history, history of smoking, drinking, body mass index, PPI, HDL - C and FBG between two groups. The systolic pressure, diastolic blood pressure, pulse pressure, TG, TC, LDL - C and vWF, CF Ⅷ levels in acute coronary syndrome group were higher than those in the control group (all P < 0.05). The levels of plasma vWF and CFⅧ were positively correlated with pulse pressure and PPI, it also was positively correlated between the levels of plasma vWF and CFⅧ (P < 0.01). Conclusion  The endothelial function was significantly impaired in Xinjiang Uygur patients with acute coronary syndromes, and vascular endothelial damage was positively correlated with pulse pressure and PPI. The pulse pressure and PPI can be used to evaluate the vascular endothelial function indirectly. Related Articles | Metrics

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Analysis of the factors related to the results of plasm D dimmer test in acute aortic syndrome GUO Qian yu,LU Cheng zhi,XIA Da sheng 2014, 42 (10):  1005-1007 .  Abstract ( 756 )   PDF (311KB) ( 2890 )   [Abstract] Objective  To explore the factors that could affect plasma level of D-dimmer test in acute aortic syn? drome. Methods  Blood samples (2 mL) from acute aortic syndrome patients (n=76) obtained immediately after admission to detect D-dimmer using ELISA. Blood routine test and biochemical indicators tests including creatinine were also performed. White blood cell (WBC), serum value of creatinine, aortic contrast-enhanced CT, incidence of Shock and death were all re? corded. The receiver-operating characteristic curve (ROC) was established to assess the potency of D-dimmer to predict hospital mortality. Results  According to ROC analysis, the optimal cut-off value of D-dimmer to predict hospital mortality was >2 988.6 μg/L (FEU), with 86.7% sensitivity and 70.5% specificity. The patients were divided into group A (D-dim? mer < 2 988.6 μg/L FEU, n=45) and group B (D-dimmer ≥ 2 988.6 μg/L FEU，n=31). Onset timing was longer in group A than that in group B（P < 0.01）. Involvement of ascending aorta was less common in group A than in group B（P < 0.05）. Aortic intramural hematoma was less common in group A than in group B（P < 0.05）. Logistic analysis demonstrated that short time of onset, involvement of ascending aorta, non-aortic intramural hematoma were all independent factors of higher D- dimmer (≥2 988.6 μg/L FEU). Conclusion  Patients with long time of onset, without involvement of ascending aorta, with intramural hematoma are liable to have lower values of plasma D-dimmer. Related Articles | Metrics

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Analysis of Prognostic Risk Factors in Patients with Community Acquired Pneumonia Complicated with Acute Kidney Injury LI Jia rui 2014, 42 (10):  1008-1011 .  Abstract ( 554 )   PDF (313KB) ( 2797 )   [Abstract] Objective  To explore clinical characteristics and prognostic risk factors in patients with community ac? quired pneumonia（CAP）complicated with acute kidney injury（AKI）．Methods  In total, 456 CAP patients were included based on the diagnostic guide．According to whether the patients were accompanied with AKI，the patients were divided in? to two groups（non-AKI group and AKI group）．AKI group were further divided into risk group, injury group and failure group by RIFLE criteria using admission creatinine．Severity in CAP patients，clinical indexes and prognostic evaluation in? dexes were compared between different groups． Multiple factors were analyzed using Logistic regression model，survival RIFLE criteria in prognostic evaluation．Results  Thirty percent（135）of the total 456 CAP patients were accompanied with AKI．Patients in AKI group were further divided into risked group (45.2%, 61 patients), injury group (17%, 23 pa? tients) and failure group (37.8%, 51 patients) according to the RIFLE diagnostic criteria using basal creatinine level. Among the 300 patients with PSI gradeⅠ to Ⅲ，23.3%（70）of patients developed AKI while among 156 patients who are with PSI grade Ⅳor over, 65 patients (41.7%) developed AKI（P < 0.01）．The 30-day mortality of CAP patients accompanied with AKI were increased compared to Non-AKI group（Non-AKI：6.2%；Risk：14.8%；Injury：21.7%；Failure：45.1%）．With de? teriorating in RIFLE criteria，the portion of patients who required mechanical ventilation, inotropic support（MV/IS）and re? nal replacement therapy（RRT）increased too. Logistic analysis revealed that AKI，age of 75 years or older and extra-renal or? gan failure were the risk factors of poor prognosis in patients with CAP. The rate of survivors was decreased in the CAP pa? tients accompanied with AKI compared with those who did not．Conclusion  There is certain incidence of AKI to compli? cate CAP patients who will have a poor prognosis．RIFLE diagnostic criteria is a valuable tool to evaluate prognosis of CAP patients complicated with AKI． Related Articles | Metrics

流行病学调查

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Knowledge, Attitudes and Practices of Sexual Dysfunction in Tianjin Reproductive Healthcare Personnels SONG Bao ji1,ZHANG Yu juan2,FU Jin peng1 2014, 42 (10):  1012-1015 .  Abstract ( 547 )   PDF (354KB) ( 2833 )   [Abstract] Objective  To better understand the knowledge, attitude, practice status of sexual dysfunction (SD) in medical staffs in field of reproductive health in Tianjin. Methods  Using random, stratified and clustered sampling approach, we selected 507 medical staffs in field of reproductive healthcare in Tianjin. Self-administered questionnaire on sexual dysfunction KAP was employed to survey the general condition, to understand SD knowledge and their attitude to SD, to perceive SD prevalence of the medical staffs and their demand to sexual dysfunction training. Results  The people who respond to our survey had high awareness to SD, but they are lack of knowlege in mechanism on sexual behavior and female SD related knowledge. Multiple-factor analysis indicated that young age group respondants（β=-0.827，P=0.018）and medical staffs from high-tier medical institutions (β=-0.223, P=0.048) showed higher awareness. When they suffered SD, 49.28% respondants would see doctor immediately, 36.85% informants chosen temporary observation, and 9.73% informants shown negative altitude. Minority (17.14%) of medical staff are able to confirmed SD to themselves and 90.48% of medical staff demand training on SD related knowledge. Conclusion  It’s necessary to perform continuing education to medical staffs via different intervention, so as to improve their cognitive level and attitude to SD, and these can help them providing better service to their patients and the public. Related Articles | Metrics

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Association of Body Mass Index, Waist Circumference and Serum Lipid Levels with Essential Hypertension 2014, 42 (10):  1016-1019 .  Abstract ( 774 )   PDF (358KB) ( 3215 )   [Abstract] Objective  To explore the relationship of different body mass index, waist circumference and serum lipid levels with essential hypertension among residents of Ningbo. And to explore the predictive value of BMI and WC (Waist Circumference) for hypertension. Methods  Adopting cluster sampling, 2 303 people (1 081 cases of hypertension and 1 222 people with normal blood pressure) were collected in the four townships of Ningbo City. We conducted questionnaires, physical examination and blood lipid testing for all of subjects. WC group: WC≥85 cm in male or WC≥80 cm in female is central obesity, and the others are normal. BMI groups: BMI≥24 kg/m2 is overweight, and BMI <24 kg/m2 is normal. The relationship of BMI, WC and serum lipid levels with hypertension was analyzed by multivariate unconditional logistic regression model. The predictive value of BMI and WC for hypertension was analyzed by receiver operating characteristic (ROC) curve. Results  WC, BMI, total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) in hypertension group were higher than those in control group (P < 0.01). And WC, BMI, TG, LDL-C is the impact factor for hypertension. The overweight rate and central obesity rate in hypertension group were higher than control group (P < 0.01). TG and LDL-C level abnormality, overweight and central obesity were the risk factors of hypertension. As a risk factor of hypertension, overweight with central obesity group is higher than overweight with normal waistline group, simple central obesity group and normal group. In the area under the ROC curve, BMI is higher than WC in both male and female population. Conclusion  WC, BMI, TG and LDL-C abnormalities were risk factors for hypertension, and BMI was a better predictive factor for hypertension than WC. Related Articles | Metrics

药物临床观察

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Clinical Analysis of Hydroxychloroquine Combined with Low-Molecular-Weight Heparin and Glucocorticoids in Treatment of Pregnant Women with Antiphospholipid Syndrome QI Li ,WANG Xiao fei,ZHANG Xiao li 2014, 42 (10):  1023-1025 .  Abstract ( 718 )   PDF (316KB) ( 2899 )   [Abstract] Objective  To investigate the safety and efficacy of hydroxychloroquine plus low-molecular-weight heparin and glucocorticoids in pregnant women with antiphospholipid syndrome (APS). Methods  Clinical data of 12 pregnant women with APS were retrospectively analyzed. Eight patients were APS secondary to connective tissue disease while four were primary APS. Hydroxychloroquine plus low-molecular-weight heparin was employed for the treatment of all the patients，among some of whom undergone combined glucocorticoids therapy. Results  All patients underwent a successful delivery with no abnormalities and are with normal Apgar scores of the babies. No compliations were developed during a follow up of 2-15 months. Conclusion  Hydroxychloroquine plus low-molecular-weight heparin and glucocorticoids is safe and effective in treatment of pregnant women with APS, but its clinical value needs further investigate Related Articles | Metrics

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Protective effect of nicorandil on myocardial injury and recurrent angina after percutaneous coronary intervention 2014, 42 (10):  1026-1028 .  Abstract ( 710 )   PDF (282KB) ( 3094 )   [Abstract] Objective  To explore the protective effect of pre-operation administration of nicorandil on myocardial injury and recurrent angina in patients with unstable angina pectoris who underwent percutaneous coronary intervention (PCI). Methods  A total of 91 patients with unstable angina pectoris were admitted for PCI and randomly divided into 2 groups who either received nicorandil（5 mg tid）or not for 7 days prior to the procedure and routine dose of nicorandil (5mg tid) after it．The concentrations of creatine kinase MB (CK-MB) and cardiac troponin I(cTnI) were compared between two groups before PCI and 6 hours, 18 hours and 24 hours after PCI；Recurent angina and major adverse cardiac events，includ? ing death，re-infarction and stroke at 6 months after the procedure were also chased and compared.  Results In total, 75 patients who were successfully undergone elective PC1 were finally enrolled，among whom 37 cases were in nicorandil group and 38 cases were in routine group． Post-procedural levels of CK-MB and cTnI significantly reduced in the nicorandil group between 6～24 h (P < 0.05) compared those in routine group．At 6 months follow-up，symptoms of recurrent angina after PCI were significantly relieved in the nicorandil group compared with that in routine group (P < 0.05)．Conclusion nicorandil can limit the PCI-induced myocardial injury and relieve the symptoms of recurrent angina after PCI in patients with unstable angina． Related Articles | Metrics

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Effects of Salmeterol/Fluticasone on Bone Metabolism and Bone Density in the Patients with Moderate Chronic Obstructive Pulmonary Disease(COPD) HAN Xue mei,LAI Yan ping,YANG Xia 2014, 42 (10):  1029-1031 .  Abstract ( 691 )   PDF (290KB) ( 2869 )   [Abstract]  Objective To observe the effects of salmeterol/fluticasone on the bone metabolism and bone density of the patients with moderate COPD and whether the effects is relate with inhaled corticosteroid course. Methods Sixty patients with COPD were divided into 2 groups by random digit table. Patients in experimental group (n=30) were given conventional therapy with salmeterol/fluticasone one inhale（bid) for continuously 12 months while patients in control group (N=30) were given conventional therapy without any glucocorticoid．The bone mineral density (BMD) of the femoral triangle in all patients were measured by DXA before therapy, 3 months and 12 months after therapy. At the same time, biochemical indicators that are correlated with bone metabolism include bone gla protein (BGP) and alkaline phosphatase (ALP) were measured by radioimmunoassay.  Results The BMD, BGP and ALP of patients in experimental group and in control group before therapy, 3 month and 12 month after therapy were compared. There is no significant difference (P＞0.05) between these two groups before therapy and after 3 month but significant difference was shown after 9 months (P＜0.05). Conclusion  Short period inhaling salmeterol/fluticasone in patients with COPD is safe. But long-term inhaling salmeterol/fluticason may lead to decline of the bone density in patients with COPD. Related Articles | Metrics

应用研究

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To Evaluate the Efficacy of Active-wing Appliance in Patients with Lingual Tipping Deep Overbite Malocclusion GUO Yi ,CHEN Jing jing,PENG Cheng ,LI Tian cheng 2014, 42 (10):  1032-1036 .  Abstract ( 624 )   PDF (732KB) ( 2896 )   [Abstract] Objective  To investigate and evaluate the change and efficacy of Active -wing Appliance in patients with lingual tipping deep overbite of Angle classⅡdivision 2 malocclusion．Methods  Twenty patients with lingual tipping deep overbite of Angle class Ⅱ division 2 malocclusions were selected for our study. Among the twenty patients, fifteen are girls and five are boys, whose age are from 16.0 to 23.0 years old with average of 18.8 years old . The patients were treated with Active -wing technique and all of them were non-extracted．Cephalometric films were taken before and after treatment to assess the effect of treatment . Eleven angular and thirteen linear measurements were taken. Paired t test was performed to analyze and evaluate the effects before and after treatment. Results  Active-wing Appliance have advantage of easy operating with short treatment cycle for treatment of deep overbite of Angle class Ⅱ division 2 malocclusion. It only takes 11 months in average for the treatment. After treatment, mandibular plane angle were increased (23.00°±6.76° vs 21.59°± 5.32°)，overbite were decreased [ (1.02±0.81) mm vs (5.67±1.22) mm] to reach normal level; incisors were labial incline sig? nificantly with increased protrusion and inclination; Post treatment parameter improvement also include: U1-NA distance in? creased (4.67±1.21 ) mm vs (1.24±1.37) mm; U1-NA angle increased (24.40°±6.36° vs 11.70°±6.87°); lower incisors were significantly intruded[ (37.16±1.81) mm vs（38.90±1.84）mm]; L6-MP were extruded [(31.68±2.87) mm vs (30.38±3.45) mm]; The cuspid and molar relationships were changed from Class Ⅱ to ClassⅠ occlusion. Conclusion  The Activewing Appliance can quickly open overbite and correct incisor lingual tipping for Angle class II division 2 with lingual tipping deep overbite. It is easy to operate and has been improved as efficient and shortened treatment. Meanwhile, it also saves arch wire. The Active-wing Appliance is especially powerful for lingual tipping deep overbite. However, the deficiency in torque control of premolars might be noticed. Related Articles | Metrics

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The Effect of Steam Sterilization on Cyclic Fatigue of Stainless Steel Files 2014, 42 (10):  1037-1039 .  Abstract ( 576 )   PDF (544KB) ( 2966 )   [Abstract] Objective  Comparing the effects of steam sterilization on cyclic fatigue of stainless steel files.  Meth ods  Fifty instruments of 25# stainless steel K files were randomly divided into 5 groups, which include 10 in each group. Group 1 was the blank control group, group 2 to 5 were subjected to 1, 3, 4, 5 steam sterilization cycles, then the files were tested by a custom-made device to assess cyclic fatigue and the number of cycles of failure (NCF) was calculated. Microstructure of each file’s fracture surface was analyzed by Scanning Electron microscope (SEM). Results  NCF in the 5 groups were 4 345.2±384.2，3 937.9±645.4，3 812.9±532.5，3 626.2±380.0，3 625.9±565.6 respectively, and the differences among 5 groups were significantly different（F=2.598, P < 0.05）. After 4 or 5 times of steam sterilization, cyclic fatigue decreased if compared with that in control group (P < 0.05). Fracture surface in group 1 and group 2 was tough dimple structure and large numbers of regular, deep dimples were distributed on the surface. You could also see micro cavities clearly formed by fracture; Fracture surface in group 4 was dimple structure in brittle intergranular morphology. It is characterized by the presence of thin brittle precipitates, clean and smooth crystal interface, and a great sense of polyhedral stereo. Conclusion  After 4 times of steam sterilization, cyclic fatigue strength of 25# stainless steel K files declined, which possessed the poten? tial risk of fracture. Related Articles | Metrics

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Stem Cells Regulation Based On the Biomechanics and the Measurement of Associated Physical Properties 2014, 42 (10):  1040-1042 .  Abstract ( 431 )   PDF (342KB) ( 2883 )   Abstract：Stem cells transplantation had been proved to be effective in many clinical diseases, however, the microenvironment wherein can influence the growth, migration and differentiation of stem cells. While the chemical microenvironment, such as hypoxia, neural growing factors and different kinds of ions, had been intensively studied, little about the physical microenvironment was presented. Mechanical forces, elasticity and rigidity of the matrix still need further investigation. This article is to summarize how the microenvironment controls the fate of stem cells, and the measurement of the mechanical properties. Related Articles | Metrics

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Redox regulation of cardiac Na and Ca handling protein LIU Yan gong1,LIU Gang 1,WANG Pu 1,ZHENG Ming qi2 2014, 42 (10):  1043-1047 .  Abstract ( 588 )   PDF (340KB) ( 2909 )   Abstract: Cardiac contractile dysfunction and arrhythmic genesis result from disturbed intracellular Na+ and Ca2+ handling in condition of oxidation stress. Stress-induced intracellular signaling regulation mechanisms in which many activated stress kinases, such cAMP-dependent protein kinase A, protein kinase C and Ca/calmodulin-dependent protein kinase Ⅱ as classical pathways, are known involved. However, it is becoming increasingly evidence that reactive oxygen species may directly oxidize these kinases, Na+ and Ca2+ channel protein and transports, leading to alter the intracellular Na+ and Ca2+ accumulation, to trigger arrhythmias. Related Articles | Metrics

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The progress of research and clinical application about miRNAs in cancer stem cells and as tumor markers ZHANG Lou qiang,SUN Geng lin 2014, 42 (10):  1048-1050 .  Abstract ( 664 )   PDF (262KB) ( 2931 )   The theory of cancer stem cells (CSCs) provides a new perspective for the study of cancer. CSCs doctrine presumes that tumor cells are heterogeneity, there are cancer stem-like cells in tumors, whose proliferation is uncontrolled. The cancer stem-like cells could form tumors at the condition of only few cells, this kind of cells only account for a very small part of all the tumor cells. The cells with stem cells characteristics could form different degree of differentiation of tumor cells and they are the root of tumor growth, recurrence and metastasis. MiRNAs are widely-existed non-coding small RNAs, which regulate 1/3 human genes and participate in a series of essential processes of life. More and more evidences indicate that miRNAs contribute significantly to cancer generation and development. They may exhibit oncogenic activity, and act as tumor suppressors. As one of post-transcriptional controlling factors, they extensively regulate biological processes of tumor related genes, and show specific miRNAs expression profiles in different cancers. The research of miRNA about CSCs has become to be forefront. Some studies have confirmed that the miRNAs play a very important role in a variety of CSCs, showing a new target for tumor therapy. Researchers have found that some specific miRNAs express in plasma of cancer patients, these miRNAs can be used as tumor markers in the patient's diagnosis and prognosis prediction. This review is focused on the current research progress about the function of miRNAs in CSCs and as tumor markers. Related Articles | Metrics