Table of Content

15 December 2013, Volume 41 Issue 12 Previous Issue    Next Issue
---
论著
实验研究
论著
短篇论著
临床论丛
综述与讲座


For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

论著

Select

Transfection of Lentivirus Vector Containing Apelin Gene into Umbilical Cord Mesenchymal Stem Cells in Vitro WANG Li 1,ZHANG Ning kun2,GAO Lian ru2,XU Xiao hong2,ZHENG Nan 2,ZHU Zhi ming2 2013, (12):  1137-1141 .  Abstract ( 691 )   PDF (938KB) ( 2695 )   [Abstract]   Objective  To construct a recombinant plasmid pUbi-apelin-FLAG-pSV40-EGFP and package with lentivirus to co-express enhanced green fluorescent protein (EGFP) and apelin, and to investigate optimal multiple of infection (MOI) to transfect human umbilical cord mesenchymal stem cells (hUCMSCs) and expression of target gene.  Methods   he apelin gene was chemically synthesized and amplified by polymerase chain reaction (PCR), and which was inserted into inear plasmid vector. The gene fragment and linear plasmid vector were connected by In-Fusion technology after enzyme digestion and transformed into competent DH5αcells. The positive clones of lentiviral expression vector were obtained after creening and followed by sequencing. The lentiviral vector was used to transfect293T cells and package virus, and then the irus titers were determined. HUCMSCs were transfected with lentivirus vector in vitro via different values of MOI. The transfection efficiency was obtained according to the optimal MOI. The expression of target gene was detected by RT-PCR and estern blot assay.  Results   A284bp target gene fragment with the restriction sites was obtained by PCR and connected to he lentiviral vector. The positive clones of lentiviral expression vector were corresponded to the expected result. The lentiviral particles were successfully packaged. HUCMSCs could be transfected by the lentivirus vector with high efficiency. The RNA and protein levels of target gene were stably up-regulated within2weeks.  Conclusion   The lentivirus vector pUbiapelin-FLAG-pSV40-EGFP can transfect apelin gene into hUCMSCs with high efficiency. The infected cells can express igh levels of apelin gene in two weeks. Related Articles | Metrics

Select

Effect of Different Degrees of Intermittent Hypoxia on inflammatory Cytokines and Adipokines in 3T3-L1 Adipocytes YANG Qing chan1,ZHOU Qin 2,WANG Yan 3,HE Qing 4,FENG Jing 3,CHEN Bao yuan5 2013, (12):  1142-1146 .  Abstract ( 614 )   PDF (560KB) ( 2728 )   [Abstract]   Objective   To study the effect of different degrees of intermittent hypoxia (IH) on inflammatory cytokines and adipokines in3T3-L1adipocytes.  Methods   An intermittent hypoxia/reoxygenation (IH/ROX) from obstructive sleep apnea (OSA) adipocyte model was established.3T3-L1adipocytes were divided into five groups, three IH groups (5% O2,7.5% O2 and10% O2, referred to as IH-1, IH-2and IH-3), sustained hypoxia group (10% O2, CH) and the normal oxygen control group (21%O2, IN). ELISA method was used to detect values of tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), leptin and adiponectin in cell supernatant. Western blot analysis was used to detect levels of hypoxia-inducible factor-1α(HIF-1α) and glucose transporter-1(Glut-1). RT-PCR assay was used to analyze HIF-1α, Glut-1, TNF-α, IL-6, leptin and adiponectin mRNA expression levels in adipocytes.   Results   The expression levels of TNF-α, IL-6and leptin  were significantly higher in IH and CH groups than those of IN group (P＜0.05). The expression levels of TNF-α, IL-6and leptin protein and leptin mRNA were significantly higher in IH-1group than those of IH-2and IH-3groups (P＜0.05). The adiponectin and its mRNA levels were significantly lower in IH and CH groups than those of IN group (P＜0.05). The adiponectin level was significantly lower in IH-1group than that of IH-2and IH-3groups (P＜0.05).  Conclusion   These results demonstrate that IH is related to the extensive changes in the expression and release of inflammation-related adipokines in cultured adipocytes. IH from OSA may underlie the development of the inflammatory response in adipocytes, which is involved in insulin resistance in patients with OSA. Related Articles | Metrics

Select

Function Study of the Nano-Molecular PAMAM as miR Carrier Targeting Gastric Adenocarcinoma SONG Wei 1,JIANG Zhong min2,XU Ping 1,LIANG Ying hong1,LIU xiao zhi3 2013, (12):  1147-1149 .  Abstract ( 702 )   PDF (767KB) ( 2711 )   [Abstract]   Objective   To study the function of the nano-molecular polyamide-amine (PAMAM) as microRNA(miR) carrier targeting gastric adenocarcinoma, and the foundation of developing an efficient delivery of small molecule drugs targeting gastric cancer thereof.  Methods   Thefolic acid (FA) /PAMAM comoles compound was prepared by dialysis method. After transfection of miR-7or liposomes into SGC-7901cell line, fluorescence microscope was used to detect the gene transfect efficiency. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the miR-7level. The immunocytochemistry assay was used to test the protein expressions of epidermal growth factor receptor (EGFR), protein kinase B (PKB) and proliferating cell nuclearantigen (PCNA). The transwell system was utilized to explore the migration ability of tumor cells.  Results   Compared with liposme, FA/PAMAN complex compound can significantly improve the level of miR-7 in SGC-7901cells，reduce the protein levels of EGFR, PKB and PCNA in SGC-7901cells, and also reduce the percentage of cancer cell migration (P＜0.05).   Conclusion   PAMAM can effectively transfect miR into gastric cancer cells, which is expected to become an efficient delivery of small molecule drugs. Related Articles | Metrics

Select

The Detection Rate and Characteristics of Primary Aldosteronism in Hypertensive Patients LEI Yong fu,LI Min 2013, (12):  1150-1152 .  Abstract ( 830 )   PDF (287KB) ( 2906 )   [Abstract]   Objective   To study the detection rate and characteristics of primary aldosteronism (PA) in patients with hypertension.  Methods   A total of197patients with hypertension were enrolled and underwent PA and other related examination to exclude the secondary hypertension. Patients were divided into two groups: PA group and essential hypertension (EH) group. The upright position test, supine position test, captopril test, venous high sodium test, potassium concentration examination and adrenal CTscanningwere detected in two groups. Values of blood aldosterone, renin activity and aldosterone to renin activity ratio (ARR) were detected in some patients.   Results   (1) In 197hypertensive patients,38were diagnosed as PA (19.29%),13were confirmed by pathology, (6cases aldosterone-producing adenoma and7unilateral adrenal hyperplasia). (2) There were no significant differences in the history of hypertension and body mass index (BMI) between two groups. There were more male patients than female patients in both groups. (3) Compared with EH group, there were relatively young age of onset, higher levels of systolic and diastolic blood pressure in PA group, but the difference was not statistically significant (P＞0.05). The levels of blood aldosterone and ARR were significantly increased in upright position and supine position in PA group (P＜0.01), but the blood level of renin activity was significantly decreased(P＜0.01). There was no significant significance in blood potassium level between two groups.  Conclusion   There was a higher detection rate of PA in patients hospitalized with hypertension. The detection rate of aldosterone-producing adenoma was similar to that of unilateral adrenal hyperplasia in patients with PA. The hypokalemia was uncommon. Related Articles | Metrics

Select

The Expressions of Musashi-2and CD133Protein in Colonic Adenocarcinoma WANG Ya fei,LIN Zhong 2013, (12):  1153-1155 .  Abstract ( 566 )   PDF (337KB) ( 2711 )   [Abstract]   Objective   To investigate the expression of Musashi-2and CD133in colonic adenocarcinoma，and their correlation with the occurrence and development of colonic adenocarcinoma thereof.  Methods   The expressions of Musashi-2and CD133protein were detected by immunohistochemical method in40colonic adenocarcinoma samples and15normal colonic structure samples. The different histological types, TNM staging, lymph node metastasis, serous infiltration, distant metastasis and expressions of Musashi-2and CD133proteins in colonic adenocarcinoma tissues were analyzed.  Results   The positive expression rates of Musashi-2and CD133protein were60% and27.5% in40colonic adenocarcinoma samples and26.7% and0in15normal colonic samples, which showed the significantly higher expression rates in colonic adenocarcinoma group than those of control group (χ2=4.850and5.156,P<0.05). There were significant differences in expressions of Musashi-2proteins between different histological stages and TNM staging (P<0.05). The positive expression rate of Musahi-2protein increased as the degree of differentiation decreased and TNM stage increased. There were significant differences in expressions of CD133proteins between different histological stages and lymph node metastasis (P<0.05). The positive expression rate of CD133protein increased as the degree of differentiation decreased and lymph node metastasis occurred.    Conclusion   The expression of Musashi-2and CD133may be related with the initiation and development of colonic cancer, which can be used as the stem cell markers of colonic adenocarcinoma for the further study. Related Articles | Metrics

Select

Effects of Smear Layer Removal on the Fracture Resistance of Roots Obturated with AH-Plus in Vitro SHENG Jun man1,LI hong bin1,LI Rong hua2,SHENG Zun qian1,YIN Kai1,3,3 2013, (12):  1156-1159 .  Abstract ( 634 )   PDF (421KB) ( 2742 )    [Abstract]   Objective   To evaluate the effects of root canal cleanlinesson the fracture resistance of roots filled with AH-Plus.  Methods  Eighty single canal premolars were instrumented using step-back technique, then were randomly divided into four groups (n=20for each group). Group A was washed with distilled water for 10min, group B1was washed with 5% EDTA for1min, group B2was washed with5% EDTA for5min and group B3was washed with5% EDTA for10min. Ten samples of each group were observed by scanning electron microscope at the coronal, middle and apical thirds to examine smear layer removal. The remaining samples of each group were fixed into a electronic universal testing machine and vertically loaded until fracture.   Results   The difference of coronal and middle thirds was significant between group B3and group B2(P＜0.05). At the middle third, there was significantly improved efficiency in smear layer removal in group B2than that of group B1(P＜0.05). The mean fracture resistance was significantly higher in group B3(391.91±12.82)N than that of group B2(335.54±16.14)N, group B1(296.47±17.82) N and group A (264.77±16.64)N (P＜0.05). Group B2showed a significantly better fracture resistance than that of group B1and group A (P＜0.05).  Conclusion   The complete removal of root canal smear layer can significantly improve the fracture resistances of roots filled with AH-Plus. Related Articles | Metrics

Select

Pathogen Epidemiology of Community-Acquired Pneumonia in Adults of Tangshan Area LIU Xiang xin 2013, (12):  1160-1164 .  Abstract ( 562 )   PDF (397KB) ( 2682 )   [Abstract]   Objective   To investigate the distribution of pathogens in adult patients with community-acquired pneumonia (CAP) in Tangshan area.   Methods   The clinical data of530hospitalized patients with CAP were retrospectively collected in department of respiratory medicine from6hospitals in Tangshan area from October2011to September2012. The sputum samples were isolated and cultured.  Results   A total of 195 strains were isolated from530patients with CAP. The most common types of pathogens includedKlebsiella pneumoniae(64strains,12.08%),Streptococcus pneumoniae(24strains,4.53%) andBauman Acinetobacter(19strain,3.58%). The detective rates ofKlebsiella pneumoniaeandPseudomonas aeruginosawere significantly higher in patients with basic diseases than those of patients without basic diseases (P<0.05). The detective rates ofKlebsiella pneumoniaeandStreptococcus pneumoniaewere significantly higher in patients not taking antibiotics than those of patients taking antibiotics (P<0.05). The detective rates of Klebsiella pneumoniae, Bauman AcinetobacterandPseudomonas aeruginosa were significantly higher in the age group≥60years than those in the age group <60years (P<0.05). The detective rates of Klebsiella pneumoniaewere significantly increased with increased levels of PORT (P<0.05). There were no significant differences in the detective rates of pathogens between different regions and different seasons (P>0.05).  Conclusion   The gram-negative bacterial pathogens were the majority isolated from patients with CAP in Tangshan area. And Klebsiella pneumoniaewas the primary bacteria. Patients with serious illness, above60years old, without antibiotic treatment before hospitalization, with basic diseases and above PORT Ⅲ levels should be given treatment of anti-gram-negative bacteria. Related Articles | Metrics

Select

Clinical Features and Antibiotic Sensitivity of Gram-Negative Diabetic Foot Osteomyelitis SHEN Xiang 1,DING Qun 1,SUN Qian 1,WANG Peng hua2,ZHU Yue jie2,LI Dai qing1 2013, (12):  1165-1168 .  Abstract ( 627 )   PDF (397KB) ( 2724 )    [Abstract]   Objective   To investigate the clinical features and antibiotic susceptibility of osteomyelitis infected by Gram-negative bacteria (G-)in patients suffered from diabetic foot ulcers (DFU).   Methods  The clinical data of 91 DFU patients accompanied with osteomyelitis (DFO) were retrospective studied. These patients hospitalized in the Tianjin Metabolic Diseases Hospital were divided into two groups, Gram-negative bacteria (G-)group (n=44) and Gram-positive bacteria (G+) group (n=42), respectively. The clinical features were compared between two groups. Logistic regression analysis was used to determine the risk factors for Gram-negative bactreial infection. The Gram-negative antibiogram was summarized.  Results   A total of112pathogens were isolated from91patients. G- bacteria were the most frequent pathogens (48.2%), following by G+ bacteria (47.3% ) and fungi (4.5% ).Pseudomonas aeruginosawas the majority of the G-bacteria. Comparing the two groups, the rate of antibiotic use within the previous6months was significantly higher in G- group (75.0%) than that of G+group (52.4%,P＜0.05). There were no significant differences in the other indicators between two groups. The Logistic regression analysis revealed that the history of antibiotic use was the independent risk factor of G-bacterial infections in DFO patients. Antibiotics susceptibilities reflected G-bacteria were more prevalent to resist to cephalosporins and quinolonem, but sensitive to imipenem, ceftazidine and cefperazone-sulbactam.  Conclusion   Gram negative bacteria were not only the main pathogens isolated from DFO patients, but also frequently resistant to several popular antibiotics in China. The proper bacteria culture and antibiotic sensitivity test are especially emphasized to patients with DFU. Related Articles | Metrics

Select

Association of Plasma Omentin-1Levels with Adiponectin and Inflammatory Cytokines in Diabetic Patients with Fatty Liver SHAO Jin shuang 2013, (12):  1169-1172 .  Abstract ( 687 )   PDF (357KB) ( 2654 )   [Abstract]   Objective   To investigate the relationship of omentin-1with adiponectin and inflammatory cytokines in type 2diabetes (T2DM) patients with nonalcoholic fatty liver disease (NAFLD).  Methods   The serum levels of omentin-1 and adiponectin were assayed by enzyme-linked immunosorbent assay (ELISA) in patients of T2DM with NAFLD (group A,n=63), T2DM without NAFLD (group B,n=63)and normal control group (group C, n=70). At the same time the biochemical markers and inflammatory marker, such as tumor necrosis factor (TNF)-α, high-sensitivity C-reactive protein (hs-CRP) and interleukin 6(IL-6) were detected in three groups. The correlation analysis and multiple regression analysis were used to detect the association of omentin-1with adiponectin and inflammatory markers. The logistic regression was used to analyze factors influencing NAFLD in patients with T2DM.   Results   The serum levels of omentin-1 and adiponectin were significantly lower in group A [ (27.02±2.82) μg/L and (11.98±3.63) mg/L] than those of group B [(31.52±2.81) μg/L and (15.85±3.28)mg/L] and group C [(35.92±2.80) μg/L and (19.88±3.44) mg/L], and there were significantly lower levels of them in group B than those of group C (P＜0.01). The plasma omentin-1level was positively correlated with adiponectin and high density li-poprotein (HDL-C) in group A. Also the plasma omentin-1level was negatively correlated with TNF-α, IL-6, fasting blood glucose (FBG), homeostasis model assessment of insulin resistance (HOMA-IR), visceral adipose tissue, waist, waist -to-hip ratio (WHR) and free fatty acid in group A (P＜0.05orP＜0.01). Multiple stepwise regression analysis showed that adiponectin, TNF-αand IL-6were independent factors influencing the level of plasma omentin-1. Logistic regression analysis showed that omentin-1was one of independent factors influencing T2DM combined with NAFLD (P＜0.01).   Conclusion   The incident of NAFLD in T2DM patients is related to the lower level of omentin-1, which may be influenced by adiponectin and inflammatory factors. Related Articles | Metrics

Select

Study on the Kill Activity of the Whole Tumor Cell Antigen Pulsed DC Co-Culture with CIK for Breast Cancer Circulating Tumor Cells Lü Yan 1,PANG Hua 2,SI Yu ling3, PANG Chun miao3,SUN Wen wen3 2013, (12):  1173-1176 .  Abstract ( 581 )   PDF (924KB) ( 2813 )   [Abstract]   Objective  To study the kill activity of the whole tumor cell antigen (Ag) pulsed dendritic cell (DC) coculture with cytokine induced killer cell (CIK) for breast cancer circulating tumor cells (CTCs).  Methods  Peripheral blood mononuclear cells (PBMC) were isolated from breast cancer patients with CTCs using a blood cell separator instrument. The epidermal cell adhesion molecule (EpCAM ) (+) breast cancer CTCs enriched by MACS were cultured in vitro. The nested RT-PCR, cell immunofluorescence imaging (CK8/18), and immunohistochemistry (CK8/18and CK19) methods, were used for the detection and identification of the cells. EpCAM (-) cells were routinely induced to DC and CIK, which were grouped into Ag-DC-CIK group, DC-CIK group, DC group and CIK group. The cytotoxic activity of co-cultured DC-CIK against breast cancer CTCs was detected by flow cytometry and MTT assay. The cell morphology was observed by light microscopy and transmission electron microscopy.   Results  The target band of CK19mRNA can be detected by nested RT-PCR. The expressions of CK8/18and CK19of EpCAM (+) cells in vitro were positive by immunofluorescence staining and immunohis?tochemical staining. The proliferative activity of co-cultured DC-CIK was higher than that of CIK (P＜0.001). The positive rates of CD1α+, CD83+CD86+, CD83+CD11C+, CD86+CD11C+DCs and CD3+CD8+, CD3+CD56+CIKs were significantly higher in Ag- DC- CIK group than those of DC- CIK group, DC group and CIK group (P＜0.05). The apoptosis of breast cancer CTCs was induced in Ag-DC-CIK, DC-CIK and CIK3groups, and apoptotic rates were (56.83±3.07)%,(31.43±1.77)% and (24.70±1.51)%, showing significant differences between them (P＜0.05). Transmission electron microscopy showed the typical micro-structure of breast cancer CTCs induced apoptosis.   Conclusion   MACS in combination with cell immunological methods can improve significantly the detective sensitivity of breast cancer CTCs. The co-cultured Ag-DC-CIK is highly effective immune cells,which shows a high er proliferation and cytoxicty against breast cancer CTCs. This may be used as a clinically immunotherapy means of anti-breast cancer recurrence and metastasis. Related Articles | Metrics

实验研究

Select

The Effects of Astragalus Injection on the Morphology and Expression of Apaf-1 in Hippocampal Neurons after Cerebral Ischemia Reperfusion in Rats LIU Rui 1,GAO Wei juan2,QIAN Tao 1,WANG Li 1 2013, (12):  1177-1179 .  Abstract ( 516 )   PDF (1054KB) ( 2694 )   [Abstract]   Objective   To investigate the effects of astragalus injection on the morphology and expression of Apaf-1 in hippocampal neurons after cerebral ischemia reperfusion in rats.   Methods   The male SD rats were randomly divided into 3groups, sham-operated group, cerebral ischemia-reperfusion group (reperfusion group) and astragalus injection intervention group (experiment group). The global cerebral ischemia-reperfusion rat model was established by Pulsinelli four-vessel occlusion method. The astragalus injection group was intraperitoneally injected with astragalus6mL/kg,30mins before surgery and repeated every24h. Rat brains were removed24h after reperfusion in each group. HE staining was used to observe the pathological changes of the hippocampal neurons under the light microscope. The ultrastructural changes of hippocam?pal neurons were observed by transmission electron microscopy. Immunohistochemistry and Western blot methods were used to measure the expression of apoptotic protease activating factor-1(Apaf-1) protein.   Results   Compared with sham-operated group, nuclear and mitochondrial damage was found in reperfusion group, and the expression of Apaf-1protein increased obviously in hippocampus（Immunohistochemistry result:0.024±0.001vs0.109±0.011;Western blot result: 0.270±0.018vs0.894±0.072, P＜0.01). Compared with reperfusion group, the damage in nuclear and mitochondria was relieved obviously in experiment group, and the expression of Apaf-1protein in hippocampus was significantly decreased (Immunohistochemistry result:0.048±0.005;Western blot result: 0.392±0.046, P＜0.01).   Conclusion   Astragalus injection can reduce pathological damage of hippocampal neurons after cerebral ischemia and reperfusion in rats, and the mechanism is related with inhibiting of Apaf-1protein. Related Articles | Metrics

Select

The Influence of Different Infusion Methods of Bone Marrow Mesenchymal Stem Cells in Adriamycin Nephropathy Rat Model BAI Yi hua 2013, (12):  1180-1183 .  Abstract ( 578 )   PDF (1476KB) ( 2647 )   [Abstract]   Objective   To investigate the effectiveness of bone marrow mesenchymal stem cell (MSC) and different transplantation methods of MSC on adriamycin (ADR) model of nephropathy in rats.   Methods   The ADR model of nephropathy was induced by left nephrectomy plus injection of ADR (2.5mg/kg) in Sprague-Dawley (SD) rats, once a week for two weeks. The model rats with nephropathy were randomly divided into three groups: adriamycin nephropathic model control group (ADR,n=12), MSCs transplantation through right renal artery group (M-A, n=12) and MSCs transplantation through peripheral veins group (M- V,n=12). Another 12SD rats were served as normal controls (N,n=12). MSCs were cultured,transplanted via right renal artery (2×106/mL) to rats in M-A group, and were transplanted via peripheral veins 2×106/mL)to rats in M-V group. The same procedure was repeated in two weeks. The blood urea nitrogen, serum creatinine, 24h urine protein and24h uromicroprotein were detected before transplantation and in one and two weeks after the second transplantation. The renal morphology and labeled cells were examined in the kidney one week after the second transplantation.  Results   The values of blood urea nitrogen, serum creatinine,24h urine protein and24h uromicroprotein were significantly higher in M-A group, M-V group and ADR group than those of N group (P＜0.01). The level of 24h uromicroprotein was significantly lower before the second transplantation in M-A group than that of ADR group (P＜0.01). The serum level of creatinine was significantly decreased in M- A group than that of ADR group and M- V group (P＜0.01). The levels of 24h urine protein and24h uromicroprotein were significantly lower after one week transplantation in M-A group than those of MV group (P＜0.01). The serum level of creatinine was significantly lower two weeks after the second transplantation in M-A group than that of ADR group and M-V group (P＜0.01), but no significant differences in the levels of urine protein and uromicroprotein between M- A group and M- V group.  Conclusion   Transplantation of MSCs can alleviate renal damage ofchronic ADR-induced nephropathy, which is more effective in rats with MSCs transplantation via renal artery than that in rats with MSCs transplantation via peripheral vein. Related Articles | Metrics

Select

Protective Effects of Folic Acid on Cardiac Development Related Genes GATA-4and NKx2.5 Expression of Offspring Rats with Maternal Coxsackievirus B3Infection XUE Ya xin1,QIU Xiao qiang2,3 2013, (12):  1184-1187 .  Abstract ( 547 )   PDF (629KB) ( 2707 )   [Abstract]   Objective   To investigate the effects of oral folic acid on cardiac development related gene expression of offspring in an experimental model of coxsackievirus B3(CVB3) infection of pregnant rats.   Methods   SD female rats were randomized into control group, folic acid group, CVB3group and CVB3+ folic acid group. The female rats were given folic acid by gavage for2weeks before pregnancy in folic acid group and CVB3+ folic acid group. After conception for7days, rat model was established by intraperitoneal injection of CVB3for5days in CVB3group and CVB3+ folic acid group. After natural childbirth neonatal heart was taken and stored in liquid nitrogen. The morphological changes of neonatal rat myocardial tissues were observed by HE staining. The expressions of GATA-4and NKx2.5mRNA were detected by RT- PCR and Western blot assay.  Results   There was significant myocardial injury, such as myocardial fiber disarray and myocardial fiber breakage, in neonatal rats in CVB3group. These damages were improved in CVB3+ folic acid group. The expression levels of GATA-4and NKx2.5genes in myocardial tissues were significantly lower in CVB3group than those of control group (P<0.05). The expression levels of GATA-4and NKx2.5proteins in myocardial tissues were significantly higher in CVB3+ folic acid group than those of CVB3group (P<0.05).   Conclusion   CVB3infection in the early pregnancy inhibited the expression of neonatal rat cardiac development factor. Folic acid supplementation has obvious protective effects on the neonatal rat cardiac development. Related Articles | Metrics

Select

The Effects of Taurine Postconditioning in Pancreatic Injury Following Limb Ischemia Reperfusion in Rats ZHENG yan ni1,MEN Xiu li1,HOU Yang yang1,SONG Xu dong2,WU Jing 1,ZHAO Jing 2 2013, (12):  1188-1190 .  Abstract ( 521 )   PDF (1354KB) ( 2659 )   [Abstract]   Objective   To observe changes of the pancreas after limb ischemia reperfusion, and the protective effect of taurine (Tau) postconditioning.  Methods   Twenty-four male SD rats were randomly divided into control group (Control), limb ischemia reperfusion (LIR) group, and taurine postconditioning (Tau) group (n=8for each group). Plasma contents of xanthine oxidase (XOD), malondialdehyde (MDA), reactive oxygen species (ROS), superoxide dismutase (SOD), pancreatic amylase (AMS), pancreatic lipase (LPS) and trypsin were detected in three groups. Morphological changes of the pancreas were observed using optical microscopy. The expressions of anti-C/enhancer-binding protein homologous protein (CHOP) in pancreas were analyzed by immunohistochemistry assay. TUNEL staining was performed to estimate the apoptosis of pancreatic cells.   Results   Compared with the control group and Tau group, plasma contents of MDA, XOD, ROS, AMS, LPS, and trypsin were significantly increased in LIR group, but the level of SOD was significantly lower in LIR group (P＜0.05). HE staining showed that acinar structure was disrupted , pancreatic lobule gap widened, stromal vascular dilatation and congestion were observed in LIR group. The perivascular infiltration of inflammatory cells appeared, islet cells were damaged with irregular islet. The immunohistochemical analysis showed the increased expression of CHOP in pancreas in LIR group than that of Tau group. And the pancreatic apoptosis was enhanced in LIR group detected by TUNEL staining.  Conclusion   Taurine postconditioning can ameliorate pancreatic injury following limb ischemia reperfusion, which may be related to the inhibition of oxidative stress, down- regulation of CHOP expression, thereby reducing endoplasmic reticulum stress- induced apoptosis. Related Articles | Metrics

Select

Effects of Simvastatin on Neurone-Specific Enolase Expression in Rats with Traumatic Brain Injury JIN Chun jie1,ZHOU Wei 2,JIANG Rong cai3,ZHANG Shi jun4,YANG Da wei4 2013, (12):  1191-1194 .  Abstract ( 632 )   PDF (487KB) ( 2662 )   [Abstract]   Objective   To study the effect of simvastatin (SIM) on the expression of neuron specific enoalse (NSE) in rat brain and serum after traumatic brain injury (TBI), and therapeutic effects of SIM on TBI thereof.  Methods   A total of 90 Sprague-Dwalye (SD) rats aged8weeks were randomly divided into sham TBI group, control group and treatment group (n=30). The TBI model was established in control group and treatment group by using Feeney method. Rats in treatment group were fed SIM10mg/kg in the evening pre-injury and in every evening post-injury while those in control group were fed the same dose of starch at the same time. Blood samples (3mL) were collected from carotid atrery in three groups, then rats were sacrificed and brains were collected at different time points (3h,12h,24h,3d,7d and14d post-injury). The serum expressions of NSE were detected by ELISA method. The NSE expressions in hippocampal area CA3were detected with immunohistochemistry.  Results   (1) In control group, the serum NSE level was significantly increased at 3h after injury, reached the peak at 3d, and was still higher than that of sham injury group at14d. In treatment group, the serum NSE level was increased3h after injury, reached the peak at24h, decreased after3d, and was near the sham injury group at14d after injury, but was significantly lower than that of control group. (2) Immunohistochemical detection showed that the NSE optical density values in hippocampal area CA3area were decreased at3h after injury in control group. The optical density values reached the lowest level between3d to7d and were still significantly lower than those of sham injury group at14d. In treatment group the optical density value was decreased at3h after injury, reached the lowest level between12h to24h and rebounded significantly at7d, then at14d up to the level of sham injury group.  Conclusion   SIM can promote the decrease of serum NSE level in TBI rats and increase the NSE expression of hippocampal neurons of injured side, showing protective effects on neuronal damage after traumatic brain injury. Related Articles | Metrics

Select

Protective Effects of Extract ofPeriplaneta Americanaon Liver Fibrosis in Rats WANG Yan rong 2013, (12):  1195-1198 .  Abstract ( 613 )   PDF (811KB) ( 2682 )    [Abstract]   Objective   To investigate the protective effects of extract ofPeriplaneta americana(APA) against carbon tetrachloride (CCl4)-induced hepatic fibrosis in rats.   Methods   Seventy SD rats were divided into five groups: normal control group (C), model control group (M), extract of APA 1group (APA1), extract of APA2group (APA2) and reduced glutathione group (R). The liver fibrosis model was induced by injecting40% CCl4-olive solution subcutaneously for seven weeks in M, APA1, APA 2and R groups. Drugs were given at the same time. Rats were killed at7-week (C, M, APA1and R groups),and were killed at9-week (APA2group). The serum values of transaminase (ALT, AST), albumin (ALB), hyaluronic acid (HA) and laminin (LN), and hepatic inflammation and fibrosis were detected respectively. The expressions of apoptotic related gene Bcl-2and Bax protein in central veins and portal areas of hepatic lobules were detected by immunohistochemical method.  Results   Rats showed poor nutritional status, and significantly increased transaminase, HA and LN in M group, but the serum level of ALB was significantly lower than that in C group. There was extensive necrosis of liver cells, obviously fibrosis or cirrhosis in model rats’liver tissues. The serum contents of ALT, AST, HA and LN were significantly decreased, the serum level of ALB were significantly increased in APA1，APA2and R groups (P＜0.05). There were complete hepatic lobule and no obvious fibrous tissue hyperplasia in liver biopsy of APA1and R groups. The values of Bcl-2/Bax proteins in central vein of liver tissues were significantly higher in APA1,APA2and R groups than those of M group (P＜0.05), but the ratio of Bcl-2/Bax was lower in periportal area of liver tissues (P＜0.05). There were significantly higher body weight and ALB levels in APA1group than those in R group (P＜0.05). The level of transaminase was slightly higher in  APA1 group than that of R group. The degeneration of liver cells was found mostly in R group.   Conclusion  The extract ofPeriplaneta americanahas a role in protecting liver cells, inhibiting the progression of hepatic fibrosis, and improving the nutritional status of trial rats. Related Articles | Metrics

Select

Puerarin Protects the Glaucomatous Retina against the Damage Induced by Oxidative Stress LIU Hai feng 2013, (12):  1199-1201 .  Abstract ( 524 )   PDF (328KB) ( 2714 )   [Abstract]   Objective   To investigate the role of puerarin in protecting the glaucomatous retina against the damage induced by oxidative stress.   Methods   Twenty- four healthy adult male rabbits were divided into three groups: control group, model group and therapy group with8rabbits per group. The glaucoma model rats of therapy group and model group were induced by injecting2.5% hydroxypropyl methylcellulose into the anterior chamber of rabbits. The therapy group was treated by injecting puerarin into the peritoneal of rabbits (10mL/kg，one time a day for21days). The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), the content and the redox state of glutathione and the contents of malondialdehyde (MDA) and nitric oxide (NO) in the retina were measured at the fourth week after the induction of glaucoma by using corresponding kits.   Results   The activities of SOD, GSH-Px and CAT, the content of reduced glutathione (GSH) and the ratio of GSH/ oxidized glutathione (GSSG) in retina were lower in model group than those of control group. The contents of GSSG, MDA and NO were higher in model group than those of control group. The activities of SOD, CAT and GSH-Px, the content of GSH and the ratio of GSH/GSSG in retina were higher in therapy group than those of model group, but still lower than those of control group. The contents of GSSG, MDA and NO were lower in therapy group than those of model group. There was no statistical difference in the content of GSSGbetween therapy group and control group.   Conclusion   Puerarin can enhance the activities of antioxidant enzymes and maintain the redox state of glutathione, which, in turn, alleviates the injury of the glaucomatous retina induced by oxidative stress. Related Articles | Metrics

论著

Select

Knowledge, Attitudes and Practices of Sexually Transmitted Diseases in Reproductive Health-Related Medical Professionals in Tianjin ZHANG Yu juan,YANG Jing ,WANG Jian mei,HUO Yan ,WANG Hui 2013, (12):  1202-1205 .  Abstract ( 572 )   PDF (356KB) ( 2639 )   [Abstract]   Objective   To understand the knowledge, attitudes, practices (KAP) and relative training demand of AIDS and other sexually transmitted diseases (STDs) in reproductive health-related medical professionals in Tianjin.  Methods   By using random, stratified and clustered sampling approach,507reproductive health-related medical professionals were selected in Tianjin area. A self-administered KAP questionnaire on AIDS and other STDs was used to collect the data included in the general condition, understanding of AIDS and other STDs knowledge, attitudes to AIDS and syphilis patients, occupational exposure of medical staffs and request of continuing education.  Results   The score of STD knowledge was (75.59±12.15) in respondents coming from the secondary medical institutions, which was significantly higher than that of tertiary medical institutions (70.11±10.07) and primary health care institutions (67.62±12.20，F=15.817，P＜0.001). Multiplefactor analysisshowed that there was a higher cognitive level for STD in professionals working in the department of obstetrics and gynecology and women’s health care (β=-3.765，P=0.048). A total of86.34% respondents worried about HIV or syphilis infection due to occupational exposure. Only 25.88% respondents expressed a willingness to provide medical services for AIDS and syphilis patients. About60.46% medical staffs experienced occupational exposure. Only13.04% respondents were familiar with emergency treatment and reporting procedures while occupational exposure occurring. The 94.62% medical personnel showed demand on relative training.  Conclusion   It’s necessary to perform continuing education to medical staffs via different intervention, so as to improve theircognitivelevel and attitudes to AIDS and other STDs, and help them providing better service to the patients and the public. Related Articles | Metrics

短篇论著

Select

Intervention Effect on Tumor Immune Escape by BuFeiXiaoJiYin combined Chemotherapy on Non-small Cell Lung Cancer 2013, (12):  1206-1208 .  Abstract ( 778 )   PDF (363KB) ( 2656 )   To investigate the intervention effect on Tumor Immune Escape by BuFeiXiaoJiYin combined Chemotherapy on middle and advanced stage of non-small cell lung cancer(NSCLC) with the Yin and Qin deficiency type. Methods: 48 cases of middle and advanced stage of non-small cell lung cancer with the Yin and Qin deficiency type were randomly divided into the treatment group (BuFeiXiaoJiYin + chemotherapy) and the control group (chemotherapy alone), every 4 weeks for a cycle, clinical symptoms, immune suppression cell and follow-up forward curative effect were observed after the two cycles. Results: The clinical symptoms of the two groups were alleviated due to treatment (P < 0.01), and the treatment group showed better than the control group（P<0.05）. The immune suppression cells of the treatment group dropped after the two cycles（P<0.01）, however, the cells grew up at the control group（P<0.05）.The immune suppression cells in the treatment group dropped obviously , compared with the control group after treatment , and there was significant difference between the two groups(P < 0.01). The median survival and the disease progressing time of the two groups was 310 d and 226, 139 d and 100 d respectively by the follow-up, 1 year accumulative total survival rates were 44% and 30.4%, the data of the treatment group are all better than the control group, while there was not statistical differences (P﹥0.05).Conclusion: BuFeiXiaoJiYin combined chemotherapy could suppress the immune suppression cell secretion and intervene tumor immune escape, Integrating modern and traditional medicine was superior to chemotherapy alone at alleviating symptoms and prolonging survival period and disease free time . Related Articles | Metrics

Select

Construction of a SOD1-G93A Eukaryotic Expression Vector by SOEing 2013, (12):  1208-1210 .  Abstract ( 593 )   PDF (333KB) ( 2846 )   [Abstract] Objective To construct the eukaryotic expression vector of SOD1-G93A gene. Methods The M1 and including mutation gene of M2 were amplified by PCR. The two amplified gene sequence was annealed to form a chimeric SOD1-G93A gene with gene splicing by overlap extension. The resulting chimera was cloned in pcDNA3.1(-) vector and verified by sequencing analysis. Results The result showed that SOD1-G93A gene was successfully amplified with gene splicing by overlap extension, and sequencing was not changed except one base compared with the GeneBank. The recombinant plasmid SOD1-G93A-pcDNA3.1(-) was identified by double digestion and sequencing results entirely consistent with the expected. Conclusion The eukaryotic expression vector of SOD1-G93A-pcDNA3.1(-) was successfully constructed. Related Articles | Metrics

Select

The Clinical Observations of Benzalkonium Chloride Combined Skin-growing Tie-an LI 2013, (12):  1211-1213 .  Abstract ( 635 )   PDF (304KB) ( 2840 )   Objective To discuss the effect of benzalkonium chloride combined skin-growing ointment in clearing bacteria and healing wound of elderly patients complicated bedsore. Methods 62 elderly patients complicated decubitus ulcer were randomly divided into experimental group(32 cases) and control group(30 cases), the local wound were applicated with benzalkonium chloride combined skin-growing ointment in experimental group, but in control group were routinely applicated with iodine combined aseptic vaseline. Results 89 strains pathogenic bacteria were collected in 62 cases of wound histological specimens, the cocci, bacilli and fungi accounted for 48%, 43%, and 9% respectly. 4 weeks later, the positive rate of wound bacteria culture in experimental group significantly were lower than that in control group(P<0.05), the defective granulation tissue volume were significantly lower than that in control group (P<0.05). 2 months later, the curative rate and total effective rate in experimental group were significantly higher than that in control group(P<0.05), but the incidence of aderse events were significantly lower than that in control group(P<0.05). Conclusion Applying the benzalkonium chloride combined skin-growing ointment is safe and effective in treating patients complicated decubitus ulcer, which can effectively kill bacteria and shorten wound healing time. Related Articles | Metrics

Select

The clinical characters of the young patients with hypertensive intracerebral hemorrhage 2013, (12):  1215-1217 .  Abstract ( 514 )   PDF (282KB) ( 2890 )   Abstract Objective The clinical characters of young patients suffering from the hypertensive intracerebral hemorrhage retained to be well understood. In order to further inspect the disease and improve its diagnosis and treatment, the data of these kind of patients in our center were collected and analyzed. Methods The clinical data of 55 young patients(≤44years) with hypertensive intracerebral hemorrhage in our center were enrolled from the Feb. of 2007 to Feb. of 2013. The MRS（Multiple Rankin Scale）was applied to evaluate their prognosis. The GCS scores at admitting, body mass index(BMI),the status of the hypertension controlling before administration, the interval term between the stroke and the administration in hospital, the location of the cerebral hemorrhage and the volume of the hematomas were all analyzed to assay their correlation with the prognosis. Results The majority of the sufferers were male. The hemorrhage most appeared in the basal ganglia. The patients with hematoma in basal ganglia and bleeding in the ventricles had worse prognosis than other ones with hematomas in other locations. Furthermore, the bad prognosis appeared in the patients with low admitting GCS, huge hemorrhage volumes, overweight and lately administration after stroke. The patients were mostly died with moderate hemorrhage in early stage because of their primary diseases. The prognosis of all the surgical treating patients with the modulate size of hematomas was good. Conclusion: Controlling the blood pressure, losing the weight, receiving conserve or surgical therapy in the early phase and expanding the surgical criteria are all the important strategies to improve the prognosis of the young patients with hypertensive intracerebral hemorrhage. Related Articles | Metrics

Select

Urokinase level in Serum and Its Relationship with Calcium in Kidney Stone Patients 2013, (12):  1217-1218 .  Abstract ( 690 )   PDF (244KB) ( 2695 )   objective To observe serum urokinase level and calcium in kidney stone patients and analysis the relevance. Methods 27 patients with calculus (stone group) and 24 healthy crowd (control group) were divided into two groups, serum urokinase level of all research object were measured by enzyme-linked immuno sorbent assay(ELISA), serum calcium level was test by biochemical detection, at the same time test renal function (serum creatinine). Results The serum urokinase level with stone group was lower than control group (P < 0.001), calcium level was higher than control group (P < 0.01); The change of of urokinase level and calcium level of stone group was negatively correlated(P < 0.01), the change of urokinase and creatinine level was negatively correlated too(P < 0.05). Conclusion The Serum urokinase level of healthy crowd was higher than patients with stone, and the urokinase may be as a new inhibitor restrain the process of stone formation by protecting renal function and reducing calcium levels. Related Articles | Metrics

临床论丛

Select

Surgical treatment of subtalar arthritis with depression fracture of the calcaneus at later period wang lu 2013, (12):  1219-1220 .  Abstract ( 875 )   PDF (347KB) ( 2674 )   【Abstract】 Objective：To approach the treatment of percutaneous hollow screw internal fixation for subtalar arthritis with depression fracture of the calcaneus at later period.Method：From March 2009 to March 2012，12 cases（13 feet） were performed percutaneous hollow screw internal fixation.The Bohier angle,the Gissane angle,post-operation fusion rate were compared and conduct the AOFAS evaluation. Result: The follow-up period ranged from 6 months to 29 months（13.1±1months），bony fusion was found in all cases（13 foots）.Function results were assessed according to AOFAS score,the average score was 85.5.There were 8 cases in excellent results，3 cases in good，1 case in fair. ll the patients were healed and their arches became normal．The average data of Bolder angle were 19.89°±1.28°after operation while 5.47°±0.37°before operation． The average data of Gissane angle were 120.28°±2.48°after operation while 154.60°±4.35°before operation.Conclusion：Percutaneous hollow screw fixation is an effective method for the subtalar arthritis with depression fracture of the calcaneus at later period，it can resume the Bolder angle and Gissane angle and alleviate the clinical symptom efficiently. Related Articles | Metrics

综述与讲座

Select

Obstructive Sleep Apnea and Endothelial Progenitor Cells 2013, (12):  1228-1232 .  Abstract ( 567 )   PDF (353KB) ( 2718 )   Obstructive sleep apnea (OSA) is independent risk factor of a variety of cardiovascular diseases. Endothelial injury could be the pivotal determinant in the development of cardiovascular pathology in OSA. Endothelial damage ultimately represents a dynamic balance between the magnitude of injury and the capacity for repair. Bone marrow-derived endothelial progenitor cells (EPCs) within adult peripheral blood presented a possible means of vascular maintenance which could home to sites of injury and restore endothelial integrity and normal function. Methods We summarized pathogenetic mechanism of OSA, searched for available studies on number and functions of EPCs in patients with OSA to explore the links between the number and functions of EPCs and OSA. Especially, we try to elucidate molecular mechanisms of effect of OSA on EPCs. Related Articles | Metrics