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15 January 2014, Volume 42 Issue 1 Previous Issue    Next Issue
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论著
短篇与病例报告
论著
短篇论著
实验研究
流行病学调查
短篇论著
临床论丛


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论著

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The inhibitory effect study of down-regulating phosphorylated HER2 on osteosarcoma cells U2-OS proliferation and metastasis in vitro. 2014, 42 (1):  1-4 .  Abstract ( 866 )   PDF (536KB) ( 3406 )   [Abstract] Objective: To investigate the effect of down-regulating phosphorylated human epidermal growth factor receptor 2 (HER2) on osteosarcoma cells U2-OS proliferation and metastasis in vitro. Methods: Various concentration of HER2 phosphorylating inhibitor Lapatinib Ditosylate (5,10,20,30,40μmol/L) were adopted to deal with human osteosarcoma cells U2-OS. MTT assay was performed to evaluate the cell proliferation during various time（24, 48, 72 h), and the IC50 value in 24h was calculated. A concentrtion value of 10umol/L below IC50 (IC50=22.15umol/L) was chosen to deal with U2-OS cells. The expression level of phosphorylated HER2 (p-HER2) was measured by western blot; Wound healing and Transwell invasion assay were performed to evaluated the cell migration and invasion abilities. Results: The HER2 phosphorylating inhibitor Lapatinib Ditosylate inhibited U2-OS cell proliferation dramatically, in which the inhibitory effect depend on the medicine concentration and duration. During 24 hours, the p-HER2 level in Lapatinib management group was lower than negative group (deal with PBS), the cell migration rate in Lapatinib management group and nagative group were (32.70±3.00)% and (94.52±4.76)% and the trans-membrane cells in two groups were 37±5/HP（×400）and 85±10/HP(×400), respectively. The differences in two groups were dramatically (P<0.05). Conclusions: Down-regulating p-HER2 in U2-OS could efficiently inhibit the cell proliferation, migration and invasion in vitro. HER2 has the potential to become a molecular target for anti-osteosarcoma metastasis. Related Articles | Metrics

短篇与病例报告

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Peripheral intrapulmonary lipoma：A case report 2014, 42 (1):  4-4 .  Abstract ( 796 )   PDF (536KB) ( 3512 )   Related Articles | Metrics

论著

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Research on effects of schisandrin B cell protective effect on the long wave ultraviolet damage immortal human keratinocytes 2014, 42 (1):  5-6 .  Abstract ( 696 )   PDF (262KB) ( 3041 )   Aim to explore the schisandrin B (SchB) on the long wave ultraviolet (UVA) induced permanent resident human keratinocytes (HaCat) injury and its possi-ble mechanism. Irradiated with UVA HaCat 5Jcm2, treated with different concentrations of SchB (0.0001umol/L 0.001 umol/L 0.01 umol/L 0.1 umol/L) treatment after UVA damage of HaCat cells, the detection cell superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, lactate dehydrogenase (LDH) and nitric oxide (ON) content. After HaCat cell was irradiated by UVA, the result of which reduced the SOD, GSH-Px activity, increased LDH and ON content, treated with different concentrations of SchB , which could increase significantly the SOD, GSH-Px activity, decrease LDH , to improve the survival rate HaCat cells, especially with the protection of the 0.001 umol/L SchB do with strongest （UVA+0.001umol/L SchB to UVA UVA model group：the cell survival rate:98.39%±3.23% to 77.110%±3.33%. GSH:8.34μmol/L±0.33μmol/L to 2.54μmol/L±0.34μmol/L, SOD:7.79 U/mL±1.22 U/mL to 3.22U/mL±0.15U/mL, LDH: 4.00 U/mL±0.98 U/mL to 7.8U/mL±1.06U/mL, NO: 0.19υmol／L±0.02υmol／L to 0.38υmol±0.03υmol , the above compared between groups p＜0.05） The conclusion is that SchB can be a cert ain extent and reduce the UVA damage of HaCat cells. As the concentration decreased, its protective effect gradually increase, when it is reaching the optimum concentration (0.001 umol/L), the protective effect of reducing mechanism, oxidative damage and inhibition of SchB cells enhance the ability of anti. Related Articles | Metrics

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Effects of intra-abdominal implantation fluorouracil sustained release implant on the expressions of survivin/caspsae-3、CD44V6 in patients with colorectal cancer 2014, 42 (1):  7-9 .  Abstract ( 672 )   PDF (532KB) ( 2950 )   Abstract Objectives:To observe the pre-and post-operational changes of the expressions of survivin/caspsae-3、CD44V6 in patients with colorectal cancer after intra-abdominal implantation fluorouracil sustained release implant. Methods:The peripheral blood levels of survivin/caspsae-3 were detected by RT-PCR and CD44V6 by FCM in 64 patients (fluorouracil sustained release implant were implanted intra-abdominally in 32 patients undergoing radical colorectal cancer surgery in the experimental group,while 32 cases undering radical surgery only served as controls) diagnosed with colon cancer.Results:No significant difierences of survivin/caspsae-3、CD44V6 were detected between the two groups before operation(P>0.05).survivin、CD44V6 levels were lower (P<0.05)while caspase-3 were higher(P<0.05) in the patients of the experimental group than the controls after operration.Conclusion:Fluorouracil sustained release implant could inhibit the expression of suvivin and raising caspase-3 to induce apoptosis,while reduce the expression of CD44V6 to effect the colorectal cancer micro transfer. Related Articles | Metrics

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Effect of Pseudolaric acid on the Expression of VEGF in Hepatocellular Carcinoma Cells 2014, 42 (1):  10-12 .  Abstract ( 717 )   PDF (302KB) ( 3296 )   【Abstract】 Objective：A study was carried to investigate the impact pseudolaric acid do on VEGF expression in human liver cancer cells BEL - 7402.Methods:Human liver cancer cell BEL -7402 were processed by 0, 5, 10, 20, 40, 80 umol?L-1 pseudolaric acid separately for 6 hours, then analysed the cell proliferation and vitality in the two ways of WST 8 and trypan blue staining , and detected the VEGF expression in human liver cancer cells BEL - 7402 using the methods of RT- PCR and Western Blotting. Results: At first, compared with the control group, there was no significant difference (P > 0.05) in liver cancer cell proliferation rate and energy that processed by different doses of pseudolaric acid. Secondly with the increasing does of pseudolaric acid , VEGF protein expression was decreased in proportion, a significant difference emerged when compared with the control group (P < 0.01) and then with the increasing does of pseudolaric acid , there were significant decrease in VEGF mRNA expression compared with the control group (P < 0.01). Conclusion: Pseudolaric acid have significant inhibiting-effect on the expression of VEGF mRNA and protein in human liver cancer cells BEL - 7402 . Related Articles | Metrics

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The Study of Correlation of CHGA and ATGL in Patients of Type 2 Diabetes Mellitus with Nonalcoholic Fatty Liver Disease Yu-Qing LIU 2014, 42 (1):  13-16 .  Abstract ( 731 )   PDF (297KB) ( 2953 )   Abstract Objective: to explore the relationship between plasma ChromograninA(CHGA ) and Adipose Triglyceride Lipase(ATGL) levels in patients of type 2 diabetes (T2DM) with non-alcoholic fatty liver disease (NAFLD).Methods: The level of the plasma CHGA and ATGL was assayed by enzyme-linked immunosorbent assay (ELISA) in all subjects, patients of T2DM with NAFLD （group A), T2DM without NAFLD（group B）and normal group（group NC). Meanwhile, analysis their relationship with various metabolic index. Results:The plasma CHGA levels of group A and group B are significantly higher than that of group NC(P<0.01) , and its level of group A is higher than that of group B (P<0.01). The plasma ATGL levels of group A and group B are significantly lower than that of group NC(P<0.01) , and its level of group A is lower than that of group B (P<0.01).Correlation analysis showed that the plasma CHGA was negatively correlated to ATGL; meanwhile,it was positively correlated to fasting insulin(FINS),HOMA-IR, free fatty acids(FFA), fat oxidation rate and negatively correlated to carbohydrate oxidation rate;the plasma ATGL was negatively correlated to BMI,FINS,TC,TG,HOMA-IR and positively correlated to FFA. Multiple stepwise regression analysis showed that FINS、ATGL and FFA were independent variables of CHGA. Logistic regression analysis showed that plasma CHGA,ATGL and FFA levels were the independent predictors of T2DM with NAFLD.Conclusion: The plasma CHGA and ATGL levels is closely correlated with substance and energy metabolism, and the interaction between them may play an important role in the pathogenesis of T2DM with NAFLD . Related Articles | Metrics

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Study on cut-off value with D-dimer for diagnosis by exclusion with venous thromboembolism in various age patients ren jing 2014, 42 (1):  17-19 .  Abstract ( 1016 )   PDF (309KB) ( 3093 )   Objective To investigate the plasma level of D-dimer in various age healthy groups, to analyze the variation characteristics of D-dimer levels with age in healthy groups, and examine the cut-off value with D-dimer of rule out venous thromboembolism in the older patients. Methods 6717 healthy individuals and 2976 hospitalized patients were selected from the Tianjin medical university general hospital from the 2011 to 2013. Using the Biomerieux VIDAS fluor-euzymelinked immunoassay analyzer assay the level of D-dimer. Results Rising levels of plasma D-dimer with age in healthy group (P<0.01), and the random patients groups of various age had the same tendency. The diagnosis cut-off value with venous thromboembolism of D-dimer was 504 ng/ml in patients group aged ≤40 years, 565 ng/ml in 41~60 years group, 817 ng/ml in 61~74 years group and 1024 ng/ml in ≥75 years group. Conclusion Plasma D-dimer level increased with age, the older patients had D-dimer cur-off value higher than other age groups, but the diagnosis sensitivity was decreased obiously. Related Articles | Metrics

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The effect on functional dyspepsia 4-lead Electrogastrography of children under H.pylori infection and after eradication therapy 2014, 42 (1):  20-23 .  Abstract ( 726 )   PDF (290KB) ( 2980 )   Objective: To explore the relationships between H.pylori infection and the functional dyspepsia through gastric electrical activity tested by 4-lead Electrogastrography (EGG) , and the effect on functional dyspepsia with eradication of Hp. Methods: To measure and analyze the data of the gastric electrical activity of 163 children with FD (103 children with HP infection, 60 without HP infection and 84 with HP infection negative after therapy) and 22 healthy controls by 4-lead EGG. Results: Infection group is compared with the non-infection group, under different slow wave frequency tests, more obvious in the percentage difference of the postprandial than that of pre-prandial. No difference was found in DF、DP、DFIC and DPIC under two conditions. Infection group is compared with the eradication group, under different slow wave frequency tests, no difference was found in the percentage difference between postprandial and pre-prandial. However, the data different of DF、DP、DFIC and DPIC between the pre-prandial and postprandial is more clear. Conclusions: The severity degree of the symptoms in children’s functional dyspepsia is closely related with H.pylori infection, the eradication improves the gastric electrical activity and reliefs the postprandial symptom of children with functional dyspepsia under H.pylori infection. Related Articles | Metrics

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The Expression and Clinical significance of RhoB and E-cadherin in Non-Small Cell Lung Cancer Tissues 2014, 42 (1):  27-30 .  Abstract ( 696 )   PDF (839KB) ( 3230 )   [Abstract] Objective To investigate expression of RhoB and E-cadherin in Non-Small Cell Lung Cancer(NSCLC) and their clinical significances. Methods Immunohistochemical staining was applied to detect expression of RhoB and E-cadherin in NSCLC group and control group. Correlations of expression of RhoB and E-cadherin to clinical pathological parameters and prognosis and their correlations were analysed. Results The expression intensities of RhoB and E-cadherin in NSCLC were distinctly lower than those in control group. The expression of RhoB and E-cadherin in NSCLC demonstrated significantly positive relation(r=0.503，P<0.01). The expression of RhoB in NSCLC was significantly different in pathological type, differentiation and lymph node metastasis(P <0.05 or P<0.01).The expression of E-cadherin in NSCLC was significantly different in TNM stage, differentiation and lymph node metastasis(P <0.05 or P<0.01). What’s more, the “coordinated expression” of RhoB and E-cadherin in NSCLC was significantly different in adenosquamous carcinoma, differentiation, TNM stage and lymph node metastasis(P <0.05 or P<0.01). The 3-year survival rates were higher in patients with RhoB high expression(83.93%) than those with low expression(40.00%), while the 3-year survival rates were higher in patients with E-cadherin high expression(85.11%) than those with low expression (44.93%). Further multivariate analysis suggested that both low expression of RhoB and E-cadherin and lymph node metastasis were prognostic indicators for NSCLC(P<0.001). Conclusion The expression of RhoB and E-cadherin shows a good correlation in NSCLC. Detecting the expression of RhoB combined with E-cadherin in NSCLC tissue may give a clue on prognosis of patient. Related Articles | Metrics

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Influence of Surface Roughness of Tooth Preparation and Cement Compatibility on the Adhesive Strength 2014, 42 (1):  31-34 .  Abstract ( 813 )   PDF (556KB) ( 3018 )   Objective To investigate the influence of surface roughness of tooth preparation on adhesive strength of different dental cements; in turn provide some reference and recommendation on the selection of dental rotary instruments and dental cements. Methods 60 dentin samples were prepared and randomly divided into 3 groups. 3 groups of samples were grinded by diamond burs with different grit-sizes (125μm、60μm、28μm). 2 samples were randomly selected from each group and were observed under SEM for the surface microprofile. The rest 18 samples from each group were evenly divided into 3 subgroups. Each subgroup was teamed with one of 3 dental cements: zinc polycarboxylate cement (ZP), glass ionomer cement (GI) and resin-modified glass ionomer cement (RMGI). Co-Cr alloy casts were cemented onto the dentin samples, and the adhesive strength was tested. Meanwhile, the type of failure was recorded for each sample. Statistical analysis was conducted with SPSS16.0 software. Results Both the surface roughness of tooth preparation and the type of dental cement have statistically significant influence on the adhesive strength. However, the interaction of the two factors was not significant. Samples grinded by 125μm-grit diamond burs had the highest adhesive strength, while the ones grinded by 28μm-grid diamond burs showed the lowest. Furthermore, RMGI exhibited the higher adhesive strength compared to ZP and GI. And there was no significant difference between ZP subgroup and GI subgroup. Conclusion Adhesive strength decreases in positive relation to surface roughness of tooth preparation. The rank of cements on adhesive efficiency is: RMGI>GI≈ZP. There is no obvious compatibility revealed between particular surface roughness and dental cements. Related Articles | Metrics

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Compare the effects of Tpeak-end/QT using ibulitide with amiodarone to terminate atrial fibrillation 2014, 42 (1):  35-37 .  Abstract ( 823 )   PDF (266KB) ( 3466 )   [Abstract] Objective To study the alteration of transmural heterogeneity of repolarization and its relationship to ventricular arrhythmia in the process of cardioversion of atrial fibrillation with ibutilide.Method .87 persistent atrial fibrillation cases with less than three months duration were enrolled randomizedly in group ibulitide and amiodarone to compare the success rate of cardioversion,analyze the alteration of the QT interval and Tpeak-end/QT before treatment and within 4 hours. Result The success rate of cardioversion of the group Ibutilide was significantly higher than the amiodarone group (61.7% vs 40.7%, P <0.05). QT interval of ibutilide group was significantly prolonged after treatment than before treatment, Tpeak-end/QT increased (P <0.01, P <0.05),and restored the level before treatment in 2 hours after treatment, and 1 hour, respectively (P> 0.05).QT interval of the amiodarone group was significantly prolonged (P <0.01), and continued to 4h after treatment, but Tpeak-end/QT did not change significantly before and after treatment (P> 0.05). Conclusion The success rate of atrial fibrillation cardioversion using ibutilide was significantly higher than amiodarone.But,after administration,ibutilide increased transmural heterogeneity of repolarization,and restored within 1 hour. Namely,it increased the risk of ventricular arrhythmias within 1 hour. Related Articles | Metrics

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Correlation study of Adiponectin and Chronic Obstructive Pulmonary Disease 2014, 42 (1):  38-40 .  Abstract ( 775 )   PDF (270KB) ( 3015 )   Abstract] Objective To study the relationship between adiponectin and pati ents of chronic obstructive pulmonary disease (COPD) with pulmonary function,age and gender.Method 120 COPD patients with normal body mass index (BMI<24kg/m2 ) were selected,including 60 patients with acute exacerbations of COPDand 60 patients with stable COPD.And 60 healthy people were included as controls.The number of Male were equal to female in each group.This experiment was to detect the plasma adiponectin of these groups. Pulmonary function results were collected and FEV1/FVC, FEV1%predicted, RV/TLC were calculated in all subjects.Result The concentrations of plasma adiponectin of patients in acute exacerbations and stable of COPD were significantly higher than those of controls. (P<0.01). Lung function including FEV1/FVC and FEV1%predicted of patients in acute exacerbations and stable of COPD were significantly higher than those of controls. (P<0.01).But RV/TLC in acute exacerbations and stable of COPD were significantly lower than those of controls. The plasma adiponectin concentrations of female was higher than the male,and it had a significant difference (P<0.05).The concentrations of plasma adiponectin were negatively correlated with FEV1/FVC（r=-0.839，P<0.01）and FEV1%predicted(r=-0.633,P<0.01),but positively correlated with RV/TLC(r=0.864, P<0.01).Plasma adiponectin levels of male in controls were significant positively related (r=0.943,P<0.01)with age while female in controls,male and female in stable and acute exacerbations of COPD were no correlation with age (P>0.05).Conclusion Plasma adiponectin concentrations of patients in COPD were significantly elevated which suggested that adiponectin in COPD play an important part in pro-inflammatory and might be closely related with airway resistance.Plasma adiponectin concentrations might be exist in gender differences.Adiponectin concentration can be related with age. Related Articles | Metrics

短篇论著

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Changes of plasma cardiotrophin-1 and N terminal probrain natriuretic in elderly hypertensive patients with heart failure by phosphocreatine therapy 2014, 42 (1):  41-43 .  Abstract ( 612 )   PDF (291KB) ( 3378 )   Abstract objective To observe the changes of plasma cardiotrophin-1(CT-1) and N terminal probrain natriuretic (NT-pro BNP) in elderly hypertensive patients with heart failure by phosphocreatine therapy．Methods 76 hypertensive patients with heart failure , aged 65 or over were randomly divided into treatment group and control group (n=38, each)．The control group received routine anti-heart failure treatment. The treatment group received conventional therapy plus creatine phosphate sodium for 2 weeks. Plasma CT-1 and NT-pro BNP were determined in all subjects. Plasma CT-1 level was measured by a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). NT-pro BNP concentration was tested by Pu Rui fluorescent dry quantitative analyzer. Results Plasma CT-1 and NT-pro BNP of two groups were lower after treatment (P＜0.01). The level of Plasma CT-1 and NT-pro BNP in treatment group was significantly decreased than that in control group (P＜0.01, P＜0.05). There was statistic difference in total effective rate ( 89.47% in treatment group /71.05% in control group , P < 0.05). The time needed in improving heart failure symptoms was shorter in treatment group (P < 0.05). Conclusions Conventional therapy plus creatine phosphate sodium decrease plasma CT-1 and NT-pro BNP levels in elderly hypertensive patients with heart failure and this therapy works faster in improving symptoms of heart failure. Related Articles | Metrics

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Relationship between Expression of MMP-9 and Ki67 in Pancreatic Cancer 2014, 42 (1):  44-46 .  Abstract ( 1124 )   PDF (513KB) ( 3292 )   Abstract Objective：This study was designed to investigate the expression of MMP-9 and Ki67 in pancreatic cancer （PC）．Methods：The expression of MMP-9 and Ki67 were detected by S-P immunohistochemical methods in 100 PC patients．Results：The survival rate in patients was correlated MMP-9 and Ki67 expressions(P<0．005)．MMP-9 expressions were correlated with TNM staging and lymph node metastasis in PC (P<0．025, P<0．025)．Ki67 expressions were correlated with TNM staging, lymph node metastasis, the degree of differentiation in PC (P<0．025, P<0．005, P<0．005)． Conclusion：Combined detection of expression of MMP-9 and Ki67 in PC may have practical value in prognosis of the PC patients． Related Articles | Metrics

实验研究

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Analyzing Gene Expression in Prefrontal Cortex of Rat Treated by Nicotine via RNA Sequencing 2014, 42 (1):  47-50 .  Abstract ( 806 )   PDF (394KB) ( 3149 )   Objective To investigate the effect of nicotine on the gene expression profile in prefrontal cortex (PFC) region of rat. Methods Twenty-four adult (8-weeks old) male F344 rats were randomly divided into treatment group and control group, with 12 animals in each group. Animals in treatment group were injected with nicotine solution while those in control group were given physiological saline for 17 days. At the end of drug administration, the tissue of PFC region was sliced from the brain of each animal. RNA sequencing was utilized to analyze the gene expression profile in PFC of each animal. Bioinformatics tools including Bowtie, Tophat and Cufflinks were used to map the sequencing reads to the reference genome of rat and to measure the relative expression level of each transcript. Genes whose expression levels were significantly different between the two groups were identified; the functional categories, as well as the biochemical pathways, associated with these genes were further analyzed. Results By comparing the expression level of each transcript between the nicotine treatment group and the control group, 885 significantly differentially expressed genes were identified. These genes were enriched in multiple Gene Ontology Biological Process categories (e.g., G protein coupled receptor protein signaling pathway, protein ubiquitination, and neurotransmitter uptake) and biological pathways (e.g., insulin signaling pathway, Alzheimer's disease, and long term potentiation). Conclusion Nicotine treatment may regulate biological processes like signaling transduction and energy metabolism in PFC of rat brain. Related Articles | Metrics

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Protective effect of N-acetyl-cysteine on irradiation-induced bone marrow mononuclear cells injury 2014, 42 (1):  54-57 .  Abstract ( 794 )   PDF (449KB) ( 3062 )   Objective To observe N-acetyl-cysteine's protective effect on irradiation-induced bone marrow mononuclear cells(BMMNCs) injury, and explore the possible mechanism. Methods There were 3 groups in the research: control group,irradiation group(doses of irradiation were 1Gy and 4Gy) and irradiation with NAC group(The NAC was cocultured with BMMNCs half hour before irradiation).We added 2×106/mL BMMNCs and the RPMI-1640 medium or 2×10-5 mol/L NAC into the 2mL EP tubes according to the different requirement of groups,then all the tubes were cultured in 37 ℃ CO2 incubator for 30 mins and irradiated 1Gy and 4Gy. Mouse BMMNCs 'cell viability was measured by bioluminescence, the level of cell reactive oxygen species was measured by DCFH-DA, and the ability of colony-forming units was reflected by CFU-GM. Results After 4Gy irradiation exposure,the BMMNCs 'cell viability was (284 296.7±16 541.2) Relative Light Units,which was lower than the control group(848 586.7±61 404.4) Relative Light Units. And After 1Gy irradiation exposure , the level of cell reactive oxygen species was （6 750.0±103.5）, which was higher than control group(5 710.7±56.2)Fluorescence Value; The number of colony-forming units per 105 cells after irradiation exposure was (626.7±51.3),significantly lower than the control group (986.7±100.7).Compared to the irradiation group, when administrate NAC half hour before irradiation, the BMMNCs 'cell viability increased to (352 770.0±23 466.1) Relative Light Units , the level of cell reactive oxygen species decreased to (5 430.0±61.0) Fluorescence Value , the number of colony-forming units per 105 cells increased to(773.3±49.3),and the result has significantly difference. Conclusion NAC has protective effect on irradiation-induced BMMNCs injury，it can play the role of positive control for the following work. Related Articles | Metrics

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therapeutic mechanism of simvastatin on rat with collagen induced arthritis 2014, 42 (1):  58-61 .  Abstract ( 632 )   PDF (861KB) ( 2930 )   Objective To evaluate therapeutic effects of simvastatin on rat with collagen induced arthritis(CIA) and to supply theoretical basis for exploring new treatments of rheumatoid arthritis. Method Arthritis was induced by injecting bovine Ⅱ collagen into Wistar rat.The rat were randomly divided into three groups: normal control,CIA control,simvastatin groups.Every seven days after first injection record once arthritis index(AI) and the volumes of hind paw; Measure the changes in serum cytokine levels including TNF-α，IL-6 by ELISA and detect the expression of caspase-3 in ankle synovial tissue by immunohistochemical and use HE staining analyse ankle pathological on fortieth day. Results The serum levels of TNF-α,IL-6 and AI and the volumes of hind paw in simvastatin group were decreased on fortieth day.The expression of caspase-3 in simvastatin group was high and synovial hyperplasia was light than that of the CIA control group. Conclusion: simvastatin can decrease serum levels of TNF-α,IL-6 in CIA rat.Also can inhibite synovial hyperplasia and increse the expression of caspase-3 in ankle of the CIA rat. Related Articles | Metrics

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Establishment and Application of Hyperinsulinemic-Euglycemic Clamp Technique in the Conscious Rats 2014, 42 (1):  62-64 .  Abstract ( 819 )   PDF (540KB) ( 3280 )   Objective: To establish hyperinsulinemic- euglycemic clamp technique in conscious rats, and to explore the effect of acute infusion of lipid on insulin sensitivity of rats. Methods: Rats were catheterized in the right jugular vein and left carotid artery, and underwent a hyperinsulinemic- euglycemic clamp(HEC) with infusion of lipid for 6 hours, in contrast with continuing infusion of 5% glucose. The glucose infusion rate (GIR) and plasma FFA were measured, respectively. Results: As expected, FFA concentration increased by 17.6-fold, and glucose infusion rates (GIR) was reduced by 27% in the Intralipid group compared to the controls with the 5% glucose group. Conclusion: A rat model of insulin resistance has been successfully esabished by intravenous intralipid infusion, which can be confirmed by the HEC technique. Related Articles | Metrics

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Rhizoma Phragmitis ethanol extract in diabetic mice liver glycogen content and glycogen synthetase expression effect 2014, 42 (1):  65-67 .  Abstract ( 636 )   PDF (532KB) ( 3455 )   Abstract：objective To study Rhizoma Phragmitis ethanol extract influence on STZ induced diabetic mice liver glycogen content and glycogen synthetase. Methods Application periodic aci-Schiff reaction assay groups of laboratory mice liver glycogen content, and application reverse transcription polymerase chain reaction and Western blot assay Glycogen synthetase the expression of mice liver. Results After PAS staining the hepatic glycogen content decreased significantly after the model control group, low-dose group, high-dose group compared with model control group increased significantly (P＜0.01), high-dose group was most pronounced among them; after RT-PCR, GS mRNA is lower than other three groups of model control group, low-dose group is lower than the high-dose group; after Westen blot after test model GS protein expression in the control group was lower than other three groups, low-dose group is lower than the high-dose group. Conclusions It is the dose-dependent regulation the Rhizoma Phragmitis ethanol extract influence on STZ induced diabetic mice liver glycogen, and raising the liver glycogen synthetase expression may be one of the role of the mechanism. Related Articles | Metrics

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Therapy with Calcitonin and 1,25 Vitamin D Prevents Periodontitis-Induced Alveolar Bone Loss in Rats Chong-You XU 2014, 42 (1):  68-70 .  Abstract ( 808 )   PDF (281KB) ( 3317 )   Abstract Objective:The goal of this study was to investigate the effect of 1,25 Vitamin D and Calcitonin on experimental periodontitis through the animal experiment.Methods:40 male Wistar rats were allocated to four experimental groups according to the treatment received during 8 weeks: periodontitis group(group A), calcitonin (group B), 1,25 Vitamin D(group C), 1,25 Vitamin D plus calcitonin(group D).Eight weeks later,the animals were sacrificed and the specimens were prepared for histological analysis.clinical assessment included probing depth(PD) and aleolar bone loss(ABL).Histological examination included periodontal membrane fiber and alveolar bone inflammatory.The serum BALP and OC were measured by ELISA method.Results:Intergroup comparisons demonstrated that group D might reduce the probing depth and the alveolar bone loss was decreased compared with group A,B and C (P<0.05).The serum BALP and OC in group D was higher than group A , B and C.Conclusions:The present study demonstrates that after taken 1,25vitaminD and Calcitonin for 8 weeks experimental periodontitis rats could decreased alveolar bone loss. Related Articles | Metrics

流行病学调查

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Study on epideniology ang risk factors of Urinary calculus in Zhuozhou city 2014, 42 (1):  71-73 .  Abstract ( 700 )   PDF (302KB) ( 2985 )   [Abstract] Objective To analyze the pathogenetic charateristics and risk factors of urinary calculus in Zhuozhou city. Methods 17854 residents with the age above 15 were surveyed from 2010 to 2013, the questionnaire include sex, age, dietary habits, etc. The methods for measurement included questionnaire, face-to-face interview,clinical examination and abdominal ultrasonography. The data processing, dataset and analyses were performed using Foxpro 6.0 and Spss 17.0. Condition logistic regession was performed to investigate the association between body mass index (BMI), drinking water, eating salt food, eating sweat food, high protein food, oil, past history of urinary calculus, family history of urinary calculus and urinary calculus. Results There were 1752 urinary calculus patients among 17854 follow-up members, the incidence was 9.81%. Twenties to fifties were the high illness age stage, accounted for 48.63%. Summer and Autumn were the high incidence seasons and the most cases of urinary calculus in the city were ureternal stones, accounted for 82.02%.Condition logistic regression analysis showed BMI, drinking water, eating salt food, eating sweat food, high protein food, oil, past history of urinary calculus, family history of urinary calculus with a elebated risk of urinary calculus.（HR=2.761,95% CI=1.546～4.929；HR=1.914,95% CI=1.272～2.881；HR=3.383,95% CI=2.133～5.362；HR=4.509,95% CI=2.457～8.277；HR=2.753,95% CI=1.710～4.432；HR=2.222,95% CI=1.012～4.880；HR=2.763,95% CI=1.534～4.948；HR=2.756,95% CI=1.682～4.453）. Conclusion The illness characteristic of urinary calculus in our city is similar to that the big clan cases prerelence disease investigation in the country , controling body mass and changing the inappropriate habitual eating patterns and generalizing the use of proportioning dietary should be the main measures to prevent urinary calculus. Related Articles | Metrics

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Studies on the electroencephalogram power spectrum changes in patients with mild cognitive impairment Yang Yao 2014, 42 (1):  74-75 .  Abstract ( 626 )   PDF (796KB) ( 3060 )   Objective：To explore the relations between the electroencephalogram power spectrum changes and cerebral artery stenosis (CAS)in patients with mild cognitive impairment (MCI). Methods: A total of 71 MCI patients were divided into three groups: non vascular damage group; moderate vascular damage group and severe vascular damage group according to head magnetic resonance angiography (MRA). EEGs were recorded at rest. Relative power was separately computed for ?, ?, ?1, ?2 and ?3 frequency bands. Results: In the spectral band power the severity of CAS was associated with increased ? power and decreased ?2 power. alpha3 power. The ?/?1 ratio increasing was consistent with the severity of CAS. All the differences were statistically significant. Conclusions: Together with ?/?1 ratio,?? and ?2 have great significance in indicating the severity of CAS in MCI patients. Related Articles | Metrics

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Clinical Study of Intercostal Cryoanalgesia in Post-thoracotomy Analgesia in Aged Patients 2014, 42 (1):  76-77 .  Abstract ( 598 )   PDF (284KB) ( 3117 )   Clinical Study of Intercostal Cryoanalgesia in Post-thoracotomy Analgesia in Aged Patients Mingjiang Li1, Weidong Zhang, Xuhui Wang, Xiaoping Li, Xiang Liu, Dongmei Yan Tianjin First Central Hospital Abstract Objective：The aim of this study was to investigate the value of intercostal cryoanalgesia in aged patients undergoing thoracotomy. Methods: 190 patients（age>60） were randomized into two groups: group A (cryoanalgesia group ,n = 91) and an group B (intravenous patient-controlled analgesia group,n = 99). Subjective pain intensity was assessed on a Prince-Henry score after operation. The intensity and the incidence of postoperation pain, the analgesic requirements, as well as the blood gas values and Chronic pain were evaluated and recorded. Results：In group A there was a statistically significant improvement in pain scores, reduction in consumption of pethidne, increase in values of partial pressure of oxygen (PaO2), reduction in the incidence of complications (P<0.05). There were no significant changes in operation time and chronic pain and numbness (P > 0.05). Conclusion: Intercostal nerve cryoanalgesia could effectively relief the postoperative pain, promote postoperative recovery in elderly patients and should be commonly used. Related Articles | Metrics

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Clinical Effects of Chinese medicine-xufuzhuyu capsule on the hyper-coagulation state of cancer 2014, 42 (1):  78-79 .  Abstract ( 1064 )   PDF (250KB) ( 3234 )   Objective: To investigate the clinical effection of xufuzhuyu capsule on the hyper-coagulation state of cancer. Methods: A total of 72 cancer patients with histological diagnoses, which serological examinations showed hypercoagulable state were included in the study. The patients were randomly divided into treatment group and control group, 36 cases for each. Patients received 2.4g xufuzhuyu capsule twice a day for 28 days in treatment group, and received symptomatic and supportive treatment alone in control group. The peripheral blood APTT, PT, FIB, D-dimer levels and platelet counts were detected within a week before and after treatment Results: The peripheral blood FIB, D-dimer levels and platelet counts in treatment group were lower than that in control group (P <0.05) after treatment, and the peripheral blood D-dimer levels after treatment were higher than that befoer treatment in control group. The effective rate in treatment group was 41.7%, which was significantly higher than 8.3% in control group (P<0.05). Conclusion: Xufuzhuyu capsule can significantly improve hyper-coagulation state in cancer patients. Study more in detail on the field is of important value to guide clinical practice and drug development work. Related Articles | Metrics

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The clinical analysis of Kawasaki disease in children above 5 years 2014, 42 (1):  80-81 .  Abstract ( 963 )   PDF (297KB) ( 2965 )   Objective For early diagnosis of Kawasaki disease(KD) and to decrease the misdiagnosis rate，this article analyzed clinical features of Kawasaki disease in the children above 5 years. Methods The clinical data of children above 5 years suffering from Kawasaki disease, whom hospitalized in tianjin pediatrics hospital from 2006 to 2010,were retrospectively analyzed and compared with the data of children under 5 years with KD。The contrast items included sex ratio,clinical situation,laboratories and physical examination,atypia/incomplete KD ratio,IVIG resistant ratio and coronary artery lesion(CAL) incidence. Results There were no differences in sex ratio, atypia KD ratio,IVIG resistant ratio and CAL．Skin of perianal area appeared flush and succedented desquamation was benefit to diagnosis of KD aged above 5 years. BCG vaccination scar appeared flush was not recorded in the patients above 5 years. Incidence rate of lymphadenectasis was higher in the patients above 5 years(p<0.05).The incidence of CAL with atypia /incomplete KD aged above 5 years was higher(80%)。Conclusion Lymphadenectasis and flush succedented desquamation of perianal area were frequently observed in the children aged above 5years. Atypia /incomplete KD aged above 5 years presented higher incidence of CAL than typical patients.KD should be regarded with differential diagnosis in fever unknow cases whose age above 5 years. Related Articles | Metrics

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Nosocomial Infection in Patients Cured by Ventricle Drainage: An Analysis of 228 Cases 2014, 42 (1):  82-83 .  Abstract ( 661 )   PDF (242KB) ( 3648 )   Abstract Objective The purpose of the present retrospective single centre study is to assess the possible causes of these infections. Patients and methods Two hundred and twenty-eight patients were included in the period from January 2003 until April 2011. Patient and disease demographics,as well as EVD data, and the occurrence of infection were reviewed, compared, and included in a risk-analysis study.Results The population's mean age was 56士15 years and the sexes were equally distributed. Most frequently,the indication for EVD was hydrocephalus due to in-traventricular haemorrhage (48.2%).An infection was documented in 23.2% of all patients.Duration of EVD drainage appeared to be a risk factor for infection(>11 days: OR 4.1;95% CI 1.8一9.2, p=0.001).CSF sampling frequency was also a significant risk-factor (no sampling: OR 0.2, 95% CI 0.2-0.5, p=0.003).Conclusions We found a relatively high percentage of EVD-related infections.After multivariate analysis there appears to be a relation with duration of drainage，frequent CSF sampling and cerebrospinal fluid leakage.As a result, a new EVD protocol is proposed in our institution that we believe will decrease the number of EVD-related infections to a minimum. Related Articles | Metrics

临床论丛

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Clinical study of implant-retained overdenture with magnetic attachment on maxillary bone Xing-Le ZHANG 2014, 42 (1):  84-85 .  Abstract ( 763 )   PDF (469KB) ( 3275 )   Objective To evaluate the clinical usefulness of implant-retained overdenture with magnetic attachment on maxilla. Methods Selected 14 patients treated by implant-retained overdenture with magnetic attachment in our hospital, the masticatory efficiency of implant-retained overdenture with magnetic attachment were determinated and compared at different periods. The patient’s satisfaction were investigated 3 month afte restoration. Results The patient’s masticatory efficiency and satisfaction of implant-retained overdenture with magnetic attachment were improved distinctly（p<0.05）.Conclusions The clinical effects of implant-retained overdenture with magnetic attachment are very well. Related Articles | Metrics

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Laparoscopic nephrectomy for dysplastic kidneys in 21 children 2 2014, 42 (1):  86-87 .  Abstract ( 798 )   PDF (489KB) ( 2984 )   Objective To review the clinical experience of laparoscopic nephrectomy for dysplastic kidneys.Methods The clinical data of 21cases of Laparoscopic nephrectomy for dysplastic kidneys from Jan．2001 to Jun．2011 were retrospectively reviewed.Results The operations of 14 cases were successfully performed with laparoscopy,and 7cases with retroperitoneal laparoscopy.The remaining 2cases converted to open suegery.There were no intraoperative or postoperative complications;The operation time ranged from 48to 162minutes(mean 62min); The postoperative hospital stay ranged from 3 to 10days(mean 5.5days). The follow-up from 6months to10 years showed the children recovered uneventfully.Conclusions laparoscopic nephreetomy for dysplastic kidneys is safe and effective method. Related Articles | Metrics

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Retroperitoneal laparoscopic partial nephrectomy for T1b renal cell carcinoma：a report of 17 cases 2014, 42 (1):  88-89 .  Abstract ( 987 )   PDF (257KB) ( 3557 )   Abstract Objective：To investigate the safety and clinical effect of retroperitoneal laparoscopic partial nephrectomy(RLPN) for T1b renal cell carcinoma. Methods： Clinical data of 17(11 males，6 females，mean age 55（48～67）years) cases underwent RLPN for T1b renal cell carcinoma were analyzed retrospectively，and the mean tumor size was 5.3(4.3～6.8)cm. Surgical parameters(operation time，blood loss，warm ischemia time，postoperative stay time) and clinical outcomes were observed and analyzed. Results：Sixteen cases were completed successfully with only one case it converted to radical nephrectomy due to uncontrollable bleeding during operation. The average operation time was 125(105～175)min；the average warm ischemia time was 27(18～39)min；the average blood loss was 125(70～330)ml；the average postoperative stay time was 10(8～12)days. One patient had urine leakage and another had serum creatinine transient increase postoperation. All patients were very well recovered. Pathology indicated clear cell carcinoma in 14 cases，chromophobe carcinoma in 2 cases and papillary cell carcinoma in 1 case. During 4～112 months of following-up，all cases had normal renal function and had no recurrence of tumor，distant metastasis and transplant metastasis at Trocar wound. Conclusion：RLPN，which is safe and feasible，is one of operation choice for patients with T1b renal cell carcinoma，but its long-term effects still need large sample studies and long-term follow-up. Related Articles | Metrics