Abstract: Objective: To establish hyperinsulinemic- euglycemic clamp technique in conscious rats, and to explore the effect of acute infusion of lipid on insulin sensitivity of rats. Methods: Rats were catheterized in the right jugular vein and left carotid artery, and underwent a hyperinsulinemic- euglycemic clamp(HEC) with infusion of lipid for 6 hours, in contrast with continuing infusion of 5% glucose. The glucose infusion rate (GIR) and plasma FFA were measured, respectively. Results: As expected, FFA concentration increased by 17.6-fold, and glucose infusion rates (GIR) was reduced by 27% in the Intralipid group compared to the controls with the 5% glucose group. Conclusion: A rat model of insulin resistance has been successfully esabished by intravenous intralipid infusion, which can be confirmed by the HEC technique.

Key words: HEC, FFA, GIR, IR