Abstract:

[Abstract]    Objective  To explore the effects of cyclic tensile strain on the proliferation of cultured human osteoar -thritis chondrocytes.    Methods  The degenerated human cartilage came from a66-year-old patient with osteoarthritis. The degree of degeneration was assessed by pathological diagnosis. Under the sterile condition, degenerated chondrocytes werecultured by enzymatic digestion. The toluidine blue staining, safranin O staining, and immunofluorescence staining of colla-gen Ⅱ were used to identify the cultured cells. The third generation cells were seeded on the silicone rubber membrane carrier, using the EF3200mechanical tester equipped with BioDynamic bioreactor system, imposed a frequency of 0.25Hz, stain 0, 5%,10% and 15% load for 3h. The proliferation activity was detected by flow cytometry.  Results   The proliferative in-dex (PI) increased with the magnitude value of cyclic tensile strain. The most significant increase of proliferation index was found in10% group.   Conclusion T  he proliferation of human osteoarthritis chondrocytes increases under some stress

Key words: osteoarthritis, cartilage, articular, stress, mechanical, cell proliferation, cell cycle