Table of Content

15 August 2014, Volume 42 Issue 8 Previous Issue    Next Issue
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细胞与分子生物学
实验研究
临床研究
应用研究
药物临床观察
循证医学
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细胞与分子生物学

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Downregulation MicroRNA-1Can Protect H2O2 Injured Cardiomyocytes 2014, 42 (8):  737-740 .  Abstract ( 541 )   PDF (445KB) ( 2695 )   Objective To investigate the protective function and mechanism of microRNA-1 (miR-1) downregulation in H2O2 injured cardiomyocytes. Methods The experiment was divided into 4 groups:Blank, NC, H2O2 and H2O2+AS-miR-1. Blank cells had no treatment, NC cells transfected with random miRNA fragement, H2O2 and H2O2+AS-miR-1 group were defined as H2O2 injured cardiomyocytes without or with transfected antisense miR-1 oligonucleotide（AS-miR-1）. The real time PCR was used to test miR-1 expression, cell vitality and apoptosis were analyzed by MTT and flow cytometry. The target gene of miR-1 was predicted by bioinformatics, and then, the mRNA and protein expression level of Bcl-2 were detected by real time PCR and western blot. Results All index had no significant changes in Blank and NC groups. H2O2 can induce cardiomyocyte injury, increase the miR-1 level and apoptosis rate, reduce cell vitality and Bcl-2 expression level. Transfection AS-miR-1 can decrease cell apoptosis, increase cell vitality and Bcl-2 expression level. Conclusion Downregulation microRNA-1 can protect H2O2 injured cardiomyocytes by increasing anti-apoptosis factor Bcl-2 expression. Related Articles | Metrics

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Correlation between Polymorphisms ofGSTM1, GSTT1andGSTP1(rs1695) on Hematologic Toxicities with Anthracycline/Paclitaxel-Based Chemotherapy in Breast Cancer 2014, 42 (8):  741-745 .  Abstract ( 741 )   PDF (415KB) ( 2935 )   [Abstract] Objective   To examine the effects of genetic polymorphisms in GSTM1, GSTT1 and GSTP1(rs1695) genes on hematologic toxicities of breast cancer patients receiving Anthracycline/Paclitaxel-based chemotherapy. Methods   The multiply PCR technique and High Resolution Melting method were used to examine 3 gene polymorphsim in 252 female breast cancers. Results   The GSTP1(rs1695) AG/GG genotype was a risk factor for Ⅲ-Ⅳ degree of neutrophil toxicity when patients received Paclitaxel-based chemotherapy and Anthracycline-Paclitaxel-based chemotherapy(OR=6.111, 95% CI:1.526~24.469, P<0.05 and OR=9.257, 95% CI:2.903-29.522, P<0.01), while there were no statistic difference on Ⅲ-Ⅳ degree hematologic toxicities rates between GSTM1(+) and GSTM1(-), GSTT1(+) and GSTT1(-) patients receiced Paclitaxel-based chemotherapy and Anthracycline-Paclitaxel-based chemotherapy(P>0.05); there was still no statistic difference on Ⅲ-Ⅳdegree hematologic toxicities rates between GSTM1(+) and GSTM1(-), GSTT1(+) and GSTT1(-), GSTP1AA and GSTP1AG/GG patients receiced Anthracycline-based chemotherapy(P>0.05). Conclusion   The genetic polymorphisms in GSTP1(rs1695) can be a gene markers for forecasting the chemotherapy neutropenia toxicities of breast cancer patients receiving Paclitaxel-based chemotherapy. Related Articles | Metrics

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Combined SYBR Green Real-Time with Telomeric Repeat Amplification Protocol (RQ- TRAP) to Detect Telomerase Activity 2014, 42 (8):  746-748 .  Abstract ( 816 )   PDF (382KB) ( 2919 )   [Abstract] Objective   To establish methodology to detect telomerase activity based on real-time quantitative PCR technique combined with telomeric repeat amplification protocol (TRAP). Methods   RQ-TRAP system was developed by combining real- time quantitative PCR technique with conventional TRAP method. Telomerase activity was assessed and compared by RQ-TRAP assay and TRAP connected with enzyme-linked immunosorbent assay (TRAP-ELISA) respectively in12kinds of cells. Results   The RQ-TRAP method was both accurate and specified in measuring telomerase activity in a series dilution of protein extracts from293T cells. The sensitivity of this method was8cells and the amplification efficiency was98.92%. Telomerase activity was not detected in negative control group. Statistical analysis revealed a strong correlation between the two assays (r2=0.7625). Conclusion   The feasibility of RQ-TRAP was proved in this article. Compared with TRAP-ELISA, RQ-TRAP has many advantages. Apart from sample extraction and real-time PCR cycling, no other extra time-consuming steps are needed for telomerase quantification；RQ-TRAP is less costly and more rapid and reliable than TRAP-ELISA for quantification of telomerase activity and it also support high throughput. Related Articles | Metrics

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Construction of JSRV-NM Pseudovirions by High Eficiency Packaging System of the Lentivirus 2014, 42 (8):  749-751 .  Abstract ( 622 )   PDF (313KB) ( 3190 )   [Abstract] Objective   To overcome the fact that SRV- NM virus can only multiple and amplify through partially pu? rified jaagsiekte retrovirus inoculated intratracheally in sheep but it cannot be augmented usingin vitro cell culture, we con?structed JSRV-NM pseudovirions based on high efficiency packing system of lentivirus.Methods   Lentivirus of three highefficiency packing plasmids system pMD.G, pCMV-HIV8.2and pHIV-eGFP was developed, and JSRV-NM-env coatedplasmid pCMVJSRV-NM was used to substitute VSV-G virus coated plasmid pMD.G then co-transfected into293T cells toreplicate, package and produce restructured JSRV-NM pseudovirions.Gene expressionofpseudovirion was determinedthrough WPRE using real time PCR; Virus infectivity was detected through inoculating JSRV- NM pseudovirions into 24pore plates.Results   We construct JSRV-NM pseudovirions successfully based on the lentivirus system. JSRV-NM pseudo?virions can also be concentrated to higher titer (108TU/mL detected by real time PCR by ultracentrifugation without signifi?ant loss of activity. JSRV-NM and VSV-G pseudovirions infected on Hela cells (both MOI =3) respectively and no obvi?ous difference were shown on their infection efficiency detected by real time PCR.Conclusion   Based on lentivirus system,JSRV-NM pseudovirions can be multipled and amplified in293T cell culturein vitro. JSRV-NM pseudovirions is stablewithout loss its infection activity and the requirements of biological laboratory safety II was also met. JSRV-NM pseudoviri?ons will provide a useful tool for further study of JSRV-NM–env infection across species or its induction of lung adenocarci?noma. Related Articles | Metrics

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Study of Reversing Invasion of Human Gastric Cancer Cell Line BGC-823by Targeting Angiopoietin-1Using siRNA 2014, 42 (8):  752-754 .  Abstract ( 538 )   PDF (497KB) ( 2651 )   Objective To knock down the expression of angiopoietin-1 in human gastric cancer cell line BGC-823, and observe the treatment effect of reversing tumor invasion phenotype. Methods To design the siRNA sequence fragments targeting angiopoietin-1 and transfer it into human gastric cancer cell line BGC-823. RT-PCR method was used to explore the expression of angiopoietin-1 mRNA, western blot and immunofluorescence methods were used to test the expression of three invasion-associated proteins, including integrin β1, CD44V6 and Ang-1. Cell adhesion assay was employed to detect the cellular adhesion ability, matrigel and transwell plastic dual-chamber culture system for detection of cancer cell invasion. Results The siRNA sequence fragments can knock down Ang-1 mRNA level through RT-PCR results. The expression of integrin β1, CD44V6 and Ang-1 were lower significantly than control group（P<0.05）, as so as the cellular adhesion and invasion abilities（P<0.05）. Conclusion The siRNA technique targeting angiopoietin-1 can reverse the invasion phenotype of human gastric cancer cell line BGC-823, and may provide new ideas and reference for future gene therapy for gastric cancer. Related Articles | Metrics

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Prokaryotic Expression and Antifungal Activity of Human α defensin-5 Protein 2014, 42 (8):  755-758 .  Abstract ( 598 )   PDF (332KB) ( 2772 )   　　[Abstract] Objective   To construct the prokaryotic expression vector for HD-5and purify the recombinant HD-5 protein then analyze its antifungal activity.Methods   The HD-5gene was cloned by PCR, then was inserted into prokary?otic expression plasmid pQE-30Xa to construct pQE-30Xa/HD-5. After sequencing, pQE-30Xa/HD-5 gene was transformed in?toE.coliM15cells. Its expression was induced by IPTG and confirmed by SDS-PAGE. The recombinant protein was purified through Ni-NTA affinity purification system. The antifungal activity was tested by disk diffusion method. Results   HD-5 gene and pQE-30Xa/HD-5vector were obtained successfully.E.coli M15strains was used to express HD-5fusion protein.After purification, the fusion protein was confirmed by Western blot. The disk diffusion test confirmed that the fusion pro?tein can inhibit Candida albicans. Conclusion   Expression vector pQE-30Xa/HD-5was successfully constructed. The HD-5fusion protein was expressed inE.colisuccessfully, which showed a certain degree of antifungal activity. Related Articles | Metrics

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Effects of Propofol on Triggering Receptor Expressed on Myeloid cells-1 (TREM-1) by Lipopolysaccharide-Stimulated Human Monocyte cell lines 2014, 42 (8):  759-761 .  Abstract ( 488 )   PDF (308KB) ( 2830 )   [Abstract] Objective   To evaluate the effects of propofol on TREM-1 expressed on THP-1 by lipopolysaccharide-stimulated and its possible mechanisms. Methods   Cells were divided into 7 groups: one group was control, one group was exposed to LPS in final concentration of 100g/ml, one group incubated with propofol of 100μM/L, and the remaining groups were combined LPS of 100ng/ml and propofol at different dose of 12.5μM/L, 25μM/L, 50μM/L, 100μM/L respectively. Following incubation for 16 hours, concentrations of TNF-α in supernatants were measured via ELISA, TREM-1 on cells were used to undertake FACS and TREM-1 mRNA were detecetd via qRT-PCR. Results   TREM-1 on THP-1 significantly increased in group P3 and group P4 compared with group LPS (P<0.05), furthermore group P4 is higher than P3. The concentrations of TNF-α significantly (P<0.05) increased in supernatant after LPS stimulation, compared with control group, and 50μM/L or 100μM/L of propofol can significantly (P<0.05) ameliorated the concentrations of TNF-α compared with LPS-single treated group. Group P4 or group Pro has no difference (P>0.05) in TREM-1 mRNA expression, but the remaining groups significantly (P<0.05) increased the amount of TREM-1 mRNA compared with control group. Conclusions   Propofol could enhance expression of TREM-1 on surface of THP-1. Meanwhile, propofol ameliorates the concentrations of TNF-α in culture supernatant, which reduces inflammatory responses. And propofol may modulate the TREM-1 mRNA expression. Related Articles | Metrics

实验研究

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Isolation and Culture of Epidermal Stem Cells from the Third Generation of Mice That Were Knocked-out ofTerc 2014, 42 (8):  762-764 .  Abstract ( 500 )   PDF (603KB) ( 2906 )   [Abstract] Objective   To observe the difference in epidermal stem cell number and function between wild-type (WT) mice and the third generation of Terc knockout (G3Terc-/-) mice. Methods   Flowcytometry was used to analyse and sort epidermal stem cells;Quantitative real-time PCR to analyse the relative expression level of p21 in epidermal stem cells; The ability of self-renewal was reflected by the number of colonies formed by epidermal stem cells. Results   Ratio of basal and suprabasal epidermal stem cells in WT mice were 9.56% and 1.22% respectively, ratio of basal and suprabasal epidermal stem cell in G3Terc-/- mice were 17.36% and 2.92%. Relative p21 expression level in G3Terc-/- mice was 6.40 fold to WT mice; Number of colonies formed by WT mice epidermal stem cells were 280.2 per 104cell,number of colonies formed by G3Terc-/- mice epidermal stem cells were 29.28 per 104 cell, it has significant difference in the above results（p<0.05）.Conclusion   Compared to WT mice, epidermal stem cells in G3Terc-/- mice were aging. Related Articles | Metrics

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The Protective Effect of Ebselen on Mitochondrial Damage after Spinal Cord Injury in Rats 2014, 42 (8):  765-768 .  Abstract ( 671 )   PDF (391KB) ( 2696 )   [Abstract] Objective   To investigate the protective effect of ebselen on mitochondrial damage,the expression of Cytochrome C and the neuronal apoptosis after spinal cord injury in rats.Methods   Sixty adult SD rats were randomly divided into 5 groups( 12 each group).Spinal cord injury model was made by Allen's method. Sham operation group received only laminectomy; SCI group received laminectomy and spinal trauma; Saline group received saline(0.1%DMSO) after injury, methylprednisolone group received 30 mg/kg methylprednisolone,ebselen group received 10 mg/kg ebselen intraperitoneally (i.p.).The malonaldehyde (MDA) and glutathion(GSH）level at the injured sites of the spinal cord at 24 hours after trauma were detected, the expression of Cytochrome C was observed,and the neuronal apoptosis was identified by TUNEL staining.Results   The level of MDA in the ebselen group was significantly lower than that in the SCI group ,and the level of GSH was significantly elevated in the ebselen group(P<0.01).Expression of Cytochrome C in ebselen group was lower than that in SCI group by Western bloting,and the neuronal apoptosis reduced significantly(P<0.01).Conclusion   Ebselen can alleviate the peroxidation，prohibit expression of Cytochrome C and inhibit neuronal apoptosis，thus it shows a protective effect to experimental acute SCI. Related Articles | Metrics

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Hydrogen sulfide reduces kidney injury of urinary-derived sepsis by inhibiting NF-κB expression, decreasing TNF-α and increasing IL-10 levels 2014, 42 (8):  769-773 .  Abstract ( 583 )   PDF (516KB) ( 2723 )   [Abstract] Objective    to investigate the effect and mechanism of hydrogen sulfide (H2S) on kidney injury induced by urinary-derived sepsis. Methods   Rabbits were randomly divided into control group, sham group, sepsis group, NaHS 2.8 umol/kg group, and NaHS 8.4 umol/kg group, with 6 rabbits in each group. Upper urinary tract obstruction and acute infection was induced to establish sepsis model. Blood was taken for white blood cell count (WBC), creatinine (Cr) and blood urea nitrogen (BUN) analysis. Morphological changes were observed by HE staining and transmission electron microscopy. Immunohistochemical staining was used to detect TNF-α, IL-10 and NF-κB expression. Cystathionine-γ-lyase (CSE) activity was measured by spectrophotometric methylene blue method and blood H2S concentration by deproteinization. Results   Levels of WBC, Cr and BUN were significantly elevated in sepsis group than control group (P < 0.05). After treatment with NaHS, levels of WBC, Cr and BUN were significantly decreased in NaHS groups than sepsis group (P < 0.05). And pathological features of kidney injury were also alleviated by NaHS. In sepsis group, levels of TNF-α, IL-10 and NF-κB were significantly increased (P < 0.05). In NaHS groups, TNF-α and NF-κB levels were significantly inhibited whereas IL-10 level was significantly increased (P < 0.05). H2S content was significantly decreased in sepsis group whereas significantly increased in NaHS group (P < 0.05). Additionally, NaHS 8.4 umol/kg group had better effect than NaHS 2.8umol/kg group. Conclusion   Exogenous H2S reduced kidney injury of urinary-derived sepsis by inhibiting NF-κB, decreasing TNF-α and increasing IL-10. Related Articles | Metrics

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Effects of Low-Frequency Stimulation of Pedunculopontine Nucleus on Spontaneous Discharges of Globus Pallidus Internus 2014, 42 (8):  774-777 .  Abstract ( 480 )   PDF (444KB) ( 2687 )   [Abstract] Objective   To explore the mechanism of the low-frequency electrical stimulate on pedunculopontine nu? cleus to treat the Parkinson (PD) through observinge the low-frequency electrical stimulation of Pedunculopontine Nucleus (PPN) in PD rat model and the effects of neurotransmitters (GPi) neurons discharge in the medial part of the globus pallidus. Methods   Thirty SD rats were randomly assigned to the control group and the PD model group, with15in each group. PD model was established through injecting6-OHDA into Substantia nigra compact (SNc) of black rat. Effect of low frequency electrical stimulation, micro-electrophoresis glutamate (Glu) and its receptor blocking breaking agent MK-801, γ-aminobu?tyric acid (GABA) and its receptor antagonist bicuculline (BIC) on discharge of rat neuron GPi was examined using extracel?lular unit recording methods through seven glass microelectrode recording. Results   When stimulated by low frequency electrical stimulation of PPN, reactions from the control group and neuronal response GPi in PD rats were inhibited. The aver?age discharge frequency was reduced compared to pre- stimulation (P<0.01). Micro- electrophoresis and BIC Glu excite neurons while microiontophoresis MK-801and GABA restrain neurons. In the background of micro-electrophoresis BIC’s excitatory effects on neuron, low- frequency electrical stimulation on PPN reduced neuronal firing frequency. And in the background of inhibition effect of micro-electrophoresis MK-801, low-frequency stimulation PPN further restrain neuronal discharge frequency.Conclusion   Low frequency electrical stimulation inhibits GPi PPN neuronal activity probably though regulating neurons projecting to the Glu and GABA nerve pathways in GPi neuron. Related Articles | Metrics

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The Effect of Hypoxia-inducible Factor（HIF）-2alpha silence on Osteosarcoma MG-63 cells Bo ZHU 2 2014, 42 (8):  778-781 .  Abstract ( 553 )   PDF (552KB) ( 2704 )   [Abstract] Objective   To investigate the effect of HIF-2a silencing by transfection of siRNA into MG-63cells un?der hypoxia.Methods   HIF-2αexpression level in MG-63cells under hypoxia was determined by Western Blot. Small in? terfering RNA (siRNA) was used to construct MG-63/siHIF-2α（siHIF-2α）cells and control MG-63/scramble(NC) cells.The expression levels of HIF-2α, Vascular endothelial growth factor (VEGF), p-Erk/ErK and Mcl-1in MG-63, NC and si?HIF-2αcells was determined by Western Blot. NC and siHIF-2αcells were cultured under hypoxia. Cell viability was as?sessed by MTT assay. Migration was identified by scratch migration assay. Tumor formation was identified by clone formation assay. Nude mouse subcutaneous xenograft model was used to investigate tumor development in vivo.Results   Hypoxia im?proved HIF-2αexpression in MG-63cells in a time-dependent manner (F=2037.412，P＜0.001). HIF-2αexpression un?der hypoxia in siHIF-2αcells was lower than that in NC cells (P＜0.01). Cell viability of siHIF-2αcells under hypoxia for12h and24h were lower than that in NC cells (P＜0.05orP＜0.01). The relative width of scratch in siHIF-2αgroup under hypoxia for12h and24h were larger than that in NC group (P＜0.01orP＜0.01). When cell counts reach 1000-5000, the clone formation rates of siHIF-2αcells were lower than that in NC cells (P＜0.05orP＜0.01). The expression of VEGF, pErk/Erk and Mcl-1protein under hypoxia in siHIF-2αcells was lower than that in NC cells(P＜0.01). Tumor sizes, weights and density of siHIF-2αgroup in nude mice were suppressed compared with those in NC group (P＜0.01). Conclusion   Blocking HIF-2αsignal pathway warrants its investigation as a potential strategy in osteosarcoma treatment. Related Articles | Metrics

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Clinical Study of Lithium Carbonate Combined with 131I for Treatment of Graves’Disease with Leucocytopenia 2014, 42 (8):  782-785 .  Abstract ( 630 )   PDF (344KB) ( 2723 )   [Abstract] Objective   To explore the efficacy and clinical value of the 131I combined with lithium carbonate in the treatment of Graves’ disease with leucocytopenia. Methods   348 patients were randomly divided into two groups.178 patients of the experimental group were treated with131I plus lithium carbonate; 170 patients of the control group were only treated with 131I .The data of recovery rate, effective rate, thyroid 131I uptake rate, the level of thyroid hormone (free triiodothyronine, FT3 and free thyroxine , FT4）and peripheral blood leukocyte were analyzed retrospectively. Results   The recovery rate of the experimental group（87.6%） is significantly higher than the control group（71.8%）. But the effective rate of the experimental group（97.8%） is not significantly higher than the control group（94.1%）.The rate of 131I uptake 24h in the experimental group （69.75±6.79）are higher than that in the control group （44.27±3.27）after the treatment of lithium carbonate .The level of thyroid hormone(FT3, FT4) in the experimental group are significantly lower than that before treatment after 2 weeks, 1 months. However, the level of thyroid hormone (FT3, FT4) in the control group are higher than that before treatment after 2 weeks and begin to decrease after 1 month. The treatment of experimental group contribute to improve the level of peripheral blood leukocyte faster. The level of peripheral blood leukocyte was significantly higher than that before treatment after 3 months （P＜0.01）.Conclusion   The treatment of lithium carbonate combined with 131I for Graves' disease with leukopenia is safe and effective. Related Articles | Metrics

临床研究

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Changes of Erythropoietin and Hemorheology in OSAHS Patients under Positive Pressure Ventilation Intervention Kaifeng DONG 2014, 42 (8):  786-789 .  Abstract ( 549 )   PDF (293KB) ( 2665 )   [Abstract]  Objective   To observe erythropoietin (EPO) changes in severe obstructive sleep apnea hypopnea syndrome (OSAHS) patients after the noninvasive positive pressure ventilation (NPPV) treatment ;then learn more about the effects of sleep disorders on the human body. Methods   32 cases were diagnosed as moderate to severe obstructive sleep apnea hypopnea syndrome with noninvasive positive pressure ventilation, 1 and 3 months later sleep monitoring and serum erythropoietin detection were executed, sleep apnea hypopnea index (AHI), sleep mean minimum oxygen saturation (LSaO2) and serum erythropoietin (EPO) levels , and its clinical significance were analysised. Results   ① After the treatment of 3 months in patients with OSAHS whole blood viscosity , plasma viscosity, whole blood reduced viscosity, erythrocyte accumulation index , RBC, Hgb, HCT, MCH, MCV, the serum EPO, LSaO2 compared with the prior treatment ，the difference was statistically significant ( P <0.05), while compared with the control group showed no significant difference (P> 0.05), all of the above indicators in the prior treatment and control group were significantly different (P <0.05). ②after treatment for 1 month ，whole blood viscosity , plasma viscosity, whole blood reduced viscosity, erythrocyte accumulation index compared with the prior treatment had no significant difference (P> 0.05); while patients’ RBC, Hgb, HCT, MCH, MCV, LSaO2 and serum EPO compared with the prior treatment ,the difference was statistically significant (P <0.05), treatment for 3 months and the control group had no significant difference (P> 0.05). ③ Correlation analysis showed that OSAHS patients(before and after treatment)serum EPO levels and Hgb concentration , RBC were positively correlated ( all P <0. 01). AHI and serum EPO levels were positively correlated ( all P <0. 01), serum EPO levels and night LSaO2 with negatively correlated (P <0.05). Conclusion   OSAHS patients with hypoxia could induce erythropoietin increased，noninvasive positive pressure ventilation therapy can significantly improve AHI, LSaO2 , reduce the level of erythropoietin then further to reduce blood viscosity, so the early application of noninvasive positive pressure ventilation could relieve airway obstruction and its complications，for the prevention and treatment of OSAHS. Related Articles | Metrics

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Effects of Glucagon Peptide-1on Blood Pressure Variability in Diabetic Hypertensive Patients WANG Shaoqing ,ZANG Li ,WANG Li ,GAO Fang ,FU Ping 2014, 42 (8):  790-792 .  Abstract ( 602 )   PDF (291KB) ( 2709 )   [Abstract] Objective   To investigate the role of glucagon peptide-1(GLP-1) in blood pressure variability (BPV) in diabetic hypertensive patients.Methods   A total of120diabetic hypertensive patients were included in this study. GLP-1 was used for the treatment. The values of body mass index (BMI), fasting blood glucose, glycosylated hemoglobin, creatine and24-hour ambulatory blood pressure (BP) monitoring were detected before and after treatment with GLP-1for six months. The clinical indices were compared before and after treatment.Results   The values of fasting blood glucose (mmol/L:7.12±0.64vs9.19±2.78), glycosylated hemoglobin (%:7.00±0.14vs8.28±1.32) and BMI (kg/m2 :19.30±3.24vs24.5±4.53) were de?creased to the normal level after GLP-1treatment for six months (P<0.05). There were significant decrease in the 24-h sys?tolic BP (mmHg:135.02±16.57vs139.52±15.60), daytime systolic BP (mmHg:132.5±14.6vs136.44±14.24), 24-h systolic BP variability (mmHg:12.20±1.44vs12.73±1.66), daytime systolic BP variability (mmHg:11.11±1.48vs11.74±1.52), and daytime diastolic BP variability (mmHg:7.03±1.42vs7.43±1.45) after GLP-1treatment for six months (P<0.05). Conclu?sion   GLP-1can decrease BPV in diabetic hypertensive patients. Related Articles | Metrics

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Relationship between Normal Thyroid Hormone Levels with Gensini Coronary Scoring System XIAO Jianyong ,CAO Lu ,CONG Hongliang 2014, 42 (8):  793-795 .  Abstract ( 774 )   PDF (281KB) ( 3429 )   Abstrat：Objective   To study the correlation between normal level of thyroid hormone and gensini coronary scoring system．Methods   233 patients who were treated with CAG and diagnosed of patients with coronary heart disease were divided into 4 groups based on gensini coronary scoring system ,all the patients were determined kinds of index which include triiodothyronine(T3),thyronine(T4),free thyronine(FT4), free triiodothyronine (FT3), thyroid stimulating hormone (TSH) and correlation between thyroid hormone level and gensini coronary scoring system was studied by Spearman correlation analysis and Multiple regression analysis.Results   No significant difference was found in the levels of triiodothyronine(T3),thyronine(T4),freethyronine(FT4),Cholesterol(CHO)andTriglyceride(TG) between the 4 groups(P>0．05).FT3 and HDL-C decreased with the increase of the degree of coronary artery lesions（P<0.01）, AGE and LDL-C increased with the increase of the degreeof coronary artery lesions(P<0.05),there was statistical significance.Negative linear correlation was found between the gensini coronary scoring system and FT3、HDL-c,was positively correlated with age and LDL-C .both the index Was independently correlated to Gensini integral.Conclusion   the lower level of FT3 is an indepent risk factor for lesion severity of CHD． Related Articles | Metrics

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Neonatal Cow’s Milk Protein Allergy with Sepsis Like Initial Symptom LIU Yang 1,BA Shuang2,ZHANG Chen 1,SONG Lili 1,SUN Chao 1,WANG Dan 1,GUO Jing 1 2014, 42 (8):  796-798 .  Abstract ( 695 )   PDF (289KB) ( 2867 )   [Abstract] Objective   To Summarize the clinical characteristics and the experience of diagnosis and treatment in the neonatal cow's milk protein allergy(CMPA) with the original symptom similar to sepsis. Methods   10 patients with neonatal cow's milk protein allergy(CMPA) with the original symptom similar to sepsis hospitalized in our hospital from July 2009 to December 2013.The history data, clinical manifestation, laboratory examination results and the treatment outcome of them were retrospectively analyzed. Results   In 10 cases,6 cases have a family history of allergy. Main clinical manifestations in the skin and gastrointestinal symptoms, including 1 case of anaphylactic shock. IgE mediated 6 cases, acidophilic cells count (1.40±0.17)×109/L,the percentage was greater than 5%;4 cases of non IgE mediated, acidophilic cells count (0.71±0.08)×109/L,the percentage was 2-3%. Blood cultures were all negative, blood leukocyte count (24.5±3.3)×109/L,rod nucleus granulocyte/neutrophils (0.161±0.035),the platelet count (655±39)×109/L, blood interleukin (IL)-6,0.31-0.93 ug/L,C reactive protein (CRP),85-144mg/L.10 cases with extensively hydrolyzed formular or amino acid formular feeding, the clinical symptoms improved or disappeared significantly, the inflammatory indexes returned to normal. Conclusion   It is necessary to make the differential diagnosis between sepsis and neonatal CMPA，which accompanied by increased platelet and acidophil. The most important treatment of neonatal CMPA is hypoallergenic formular replacement therapy. Related Articles | Metrics

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Analysia of clinicopathological features and prognosis of gastric carcinoma with neuroendocrine differentiation and mixed gonadal neuroendocrine carcinoma of the stomach SONG Wei 2014, 42 (8):  799-801 .  Abstract ( 1211 )   PDF (301KB) ( 3076 )   [Abstract] Objective   To investigate the clinical pathological features and prognosis of gastric carcinoma with neu? roendocrine differentiation (NED) and mixed gonadal neuroendocrine carcinoma of the stomach (MANECs). Methods   A retrospective analysis of61cases of gastric carcinoma with NED and34cases of MANECs and their histochemistry and im?munohistochemistry were also observed. Prognosis of the2groups were compared by the Kaplan-Meiers survival analysis. Prognostic factors associated with patients with gastric cancer were analyzed by COX proportional hazards model.Results   Tumor location, distant metastasis and lymph node metastasis were statistically different between these2groups (P<0.05). Syn positive expression rate is higher than CgA and CD56in the gastric carcinoma with NED group; Postoperative survive pe?riod of the gastric carcinoma with NED is shorter than that of MANECs (P<0.05). Lymph node metastasis and distant region?al transfer is obviously correlated with prognosis (P<0.05). Conclusion   Immunohistochemistry is important for the diag?nosis of these two tumor. The number of neuroendocrine cells can help to assess prognosis and guide treatment. Related Articles | Metrics

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The Relationship between Orexin A and Respiratory Drive in Patients with Chronic Obstructive Pulmonary Disease QIN Yong , LI Feng ,ZHAO Tian 2014, 42 (8):  802-804 .  Abstract ( 628 )   PDF (280KB) ( 2692 )   Abstract Obejective: To explore the correlation of respiratory drive with orexin A in COPD patients. Methods: Pulmonary function test was performed in 30 patients with stable COPD and 20 normal healthy adults. The level of plasma orexin-A was measured with a radioimmunoassay kit. The correlation of orexin A with BMI, age, BDI, FEV1, FVC, FEV1/FVC, MEP, MIP, P0.1 in COPD patients was analyzed. Results: The plasma orexin-A levels in patients with COPD group （1.87±0.43）ng/L was higher than those in the control group （1.49±0.19）ng/L, P< 0.01. Plasma orexin-A levels in patients with COPD correlated negatively with FEV1（r=-0.389，P<0.05），and correlated positively with P0.1（r=0.728，P< 0.01）.Conclusions: The plasma orexin A in COPD patients are increased which may be caused by smoking and hypercapnia. Orexin A may play a crucial role in the regulation of respiratory drive in COPD patients. Related Articles | Metrics

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Study of Human Cytomegalovirus mRNA Transcription Profile at Different Infection Stages in Infants Juan LI 2014, 42 (8):  805-807 .  Abstract ( 489 )   PDF (298KB) ( 2667 )   [Abstract] Objective   Human cytomegalovirus (HCMV) can induce infection in multiple organs, including genitouri? nary system, central nervous system and liver. It can even lead to serious defects and death. China, as a high-incidence area of HCMV, need employ early treatment. However, the sensitivity of present HCMV diagnosis is not satisfied.Methods   In the present study, we focused on transcription level of HCMV IE and pp67mRNA using real- time quantitative PCR. Results   IEmRNA was positive even in a portion of patients who were regarded as negative by traditional DNA diagnosis method. On the other hand, pp67mRNA that were normally expressed at the late stage of HCMV infection was100% posi?tive in the first-visit patients who were regarded as positive using traditional DNA diagnosis method. Conclusion   The pres?ent study indicated that mRNA detection based on PCR method was more sensitive compared with DNA detection for assess?ing HCMV virus infection. Finally, different treatment should be performed upon mRNA transcription profile at different in?fection stages Related Articles | Metrics

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Clinical and Pathological Features of Canceration of Epidermoid Cyst GUO Yichuan 1,GUO Yali 2 2014, 42 (8):  808-809 .  Abstract ( 1307 )   PDF (408KB) ( 2832 )   [abstract] Objective:  To study and research the clinical pathologic features ,immunohistochemistry and prognosis of epidermoid cyst with cancerous changes. Methods:  Six cases of epidermoid cyst with cancerous changes were studied to research the clinical pathologic features , immunohistochemistic expression and evaluate the prognosis by follow-up visits .Results:   Clinical features: six cases were all local bag piece and expanded resection. Follow-up of one year, No one have recurrence and metastasis. Pathological examination: they were all for not plastic masses and cystic,something like bean curd were in the cyst. Microscopic examination: These cysts lined by squamous epithelium and they presented invasive growth. Pathological diagnosis: Epidermoid cyst canceration of squamous cell carcinoma. Immunophenotype: Squamous cell cancer of six cases were positive expressions of CK5/6 and P63. Conclusion:   Epidermoid cyst with cancerous changes are quite rare.Diagnosis of the tumor relies largely on histopathology.Immunohistochemical markers assist the diagnosis. Local appropriately expanded resection is preferred. The malignant degree is low and prognosis is good. Related Articles | Metrics

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Relationship between Acute Cerebral Infarction and Seasonal Variations in Tianjin XUE Juanjuan ,WANG Lin ,LI Xin ,TIAN Xiaolin ,LIU Peilin ,Rui FENG,WANG Luosha 2014, 42 (8):  810-813 .  Abstract ( 550 )   PDF (317KB) ( 2687 )   [Abstract] Objective   To explore the influence of seasonal variations on acute cerebral infarction outcome.Meth? ods   We collected data from acute cerebral infarction patients in the Second Hospital of Tianjin Medical University from January2009to February 2012. All cases were classified by months and seasons into different groups to analyze the season?al variations retrospectively.Results   (1) The percentage of good outcome of acute cerebral infarction show significant dif?ference between each month （P<0.01）. The percentage of good outcome was highest in August （92.1%）, lowest in Decem?ber（78.1%）, second lowest in April（81.9%）. The percentage of good outcome in summer （91.6%）was higher than spring（87.2%）, autumn （86.4%）and winter（83.9%）（P<0.05）. (2) Among those patients converted to bad outcome more pa?tients was attacked by cerebrovascular accident in winter （68.3%）than in summer （43.2%）, and more patients was combinedwith a pulmonary infection in winter（69.5%）than in summer （48.6%）and in autumn（49.3%）（P<0.05）. (3) Seasonal effect in acute cerebral infarction outcome was obvious in those ≥65years of age（P<0.05）. Conclusion   Seasonal changes are associated with the outcome and severity of acute cerebral infarction Related Articles | Metrics

应用研究

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Study of Application of Coronary Endarterectomy in Coronary Artery Bypass Graft Kai WANG 2014, 42 (8):  814-817 .  Abstract ( 608 )   PDF (611KB) ( 2699 )   [Abstract] Objective:   To study the application of coronary endarterectomy (CE) in coronary artery bypass graft(CABG) , and to evaluate the effect of CE plus CABG on patients with chronic total occlusion (CTO) combined with diffuse distal atherosclerosis．Methods:   Data from the 31 patients, who underwent coronary endarterectomy in our institution between October 2009 and October 2012, were reviewed retrospectively and compared with a control group of 31 patients matched for age, sex, LV function, and angina class. Results:   27 patients had one, four had two coronary arteries endarterectomized. Of these 35 endarterectomies, 13 (37%) the left anterior descending artery, 3(8%) branches of the circumflex artery, and 19(54%) involved the right coronary artery. There was no myocardial infarctions in the endarterectomy group and control group. All patients were followed up and no recurrent angina in the endarterectomy group. In CE group a patient died of fungal pneumonia and heart failure half a year after operation. Conclusion:   In current cardiac surgical practice, coronary endarterectomy is an indispensable adjunct to CABG. The operative mortality and major morbidity were comparable or similar to coronary artery bypass grafting, and short-term and medium-term results were favorable. Related Articles | Metrics

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Examining Circulating Growth Differentiation Factor-15Levels and Its Clinical Significance in Patients with Non-Valvular Atrial Fibrillation Hongmei LIU 1,Qing-Miao SHAO2,Enzhao LIU 3,Guangping LI3, Tong LIU3 2014, 42 (8):  818-821 .  Abstract ( 729 )   PDF (358KB) ( 2890 )   [Abstract] Objective   To investigate circulating level of growth differentiation factor-15(GDF-15) in patients with non-valvular atrial fibrillation (AF).Methods   We enrolled104consecutive patients with AF (67are paroxymal AF and37 are persistent AF) that presented to our hospital between August2012and June2013. Subjects without AF (n=67) was used as controls who were matched for sex, age and atherosclerotic risk factors enrolled in the same period. Serum GDF-15con?centration was detected byenzymelinked immunosorbent assay(ELISA). Echocardiography and laboratory parameters were compared among three groups. The correlation of GDF -15and hypersensitive c-reactive protein (hs-CRP) as well as the risk factors of development of AF were also analyzed.Results   No significant difference in age, history of hypertension, coro?nary heart disease, and stroke was found among three groups (P>0.05). GDF-15had significant difference between pa?ients with paroxysmal AF and controls [(1473.14±628.52)μg/Lv (1233.592±262.76)μg/L,P=0.012]. GDF-15level was correlated with hs-CRP (rs=0.172，P=0.044). Multivariable analysis demonstrated that high GDF-15is a risk factor of devel?opment of paroxysmal AF/AF (OR: 1.002, 95%CI: 1.000-1.003, P<0.05).Conclusion   Patients with AF have a higher se?rum level of GDF-15compared with controls and GDF-15level is a risk factor of development of paroxysmal AF/AF. Related Articles | Metrics

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Application of two kinds of video laryngoscope in general anesthesia during UPPP operation 2014, 42 (8):  822-824 .  Abstract ( 1309 )   PDF (297KB) ( 2758 )   [Abstract] Objective   To compare intubation success rate and safety of two types of video laryngoscopes when used for anesthesia in uvulopalatopharyngoplasty surgery (UPPP) on patients with obstructive sleep apnea syndrome (OSAS). Methods   60 patients undergoing UPPP surgery between January and October of 2013 were selected and randomly divided into McGrath MAC video laryngoscope group (M group), GlideScope video laryngoscope (G group), and SHUCMAN direct laryngoscopy (S group), with 20 patients per group. Mallampati classification scores, Cormack-Lehane grade, intubation success rate, pre-intubation and post-intubation heart rate and blood pressure changes were recorded and compared. Results   Mallampati classification scores were not statistically significant between three groups, Cormack-Lehane grade between M group and G group were also not statistically significant (P>0.05). M and G group had distinct advantages in Cormack-Lehane grade, success rate in intubation, and heart rate and blood pressure at completion of intubation (T3) and 1 minute after intubation (T4), the difference is statistically significant (P<0.05). Recorded blood pressure changes were more stable in G group than M group. Conclusion   The two video laryngoscopes used in anesthesia intubation during UPPP surgery can both effectively reveal the structure of the throat, with high success rate and safety, but in this study the McGrath MAC video laryngoscope was superior to GlideScope video laryngoscope. Related Articles | Metrics

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Effects of VIABAHN-covered stent and bypass in treatment of lower limb arteriosclerosis occlusion Qinghong SONG 2014, 42 (8):  825-826 .  Abstract ( 555 )   PDF (293KB) ( 3182 )   [Abstract] Objective   To evaluate the short and medium term therapeutic effects of VIABAHN-covered stent in treatment of arteriosclerosis obliterans of lower extremity. Methods   Of 53 arteriosclerosis obliterans of lower extremity cases with C/D TASC levels, 22 was performed VIABAHN-covered stent (stent group), while 31 were treated by femoral-popliteal artery bypass grafting surgery (graft group). The arterial patency rates were analyzed 3 and 6 months after the two different surgeries. Results   The arterial patency cases were 28 (90.3%) at 3 months and 26 (83.9%) at 6 months in stent group while 22 (100%) at 3 months and 21 (95.5%) at 6 months in graft group. There were no significant differences according to the arterial patency rate at 3 months and 6 months between the two different surgeries (χ2 is 0.808 and 0.760 respectively, P>0.05). Conclusion   VIABAHN-covered stent can be used as the preferred treatment for arteriosclerosis obliterans of lower extremity. Related Articles | Metrics

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Clinical Analysis of Endovascular Treatment of TASC (Ⅱ) D-Type Femoral Artery Occlusion lcunfa liu1, Xiujun ZHANG2 2014, 42 (8):  827-829 .  Abstract ( 543 )   PDF (346KB) ( 2720 )   [Abstract] Objective   To investigate methods and results of endovascular treatment in TASC (Ⅱ) D-type femoral artery occlusion.Methods   From January2012to May 2013, 26cases (26branches) of superficial femoral artery occlusion with endovascular treatment of TASC (Ⅱ) D-type superficial femoral artery occlusion were retrospectively reviewed. The effi?cacy was evaluated through ABI, CTA, DSA and symptoms improved.Results   26branches were treated with endovascular methods. Technical success rate was80.7% (21/26), including 13branche with stent implantation,6branches with Silver?hawk atherectomy and2branches with Viabahn stent implantation．All patients were followed up for a mean period of（10.3±1.2）months, primary patency rates at6months were69.2% in stent group,66.7% in Silverhawk atherectomy group and100% in Viabahn stent group.Conclusion   Endovascular treatment of TASC (Ⅱ) D-type femoral artery occlusion can lead to satisfactory short term patency rates, and Viabahn stent is the latest treatment. Related Articles | Metrics

药物临床观察

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Effect of Creatine Phosphate Sodium on Postoperative Fatigue in Gastric Carcinoma Patients 2014, 42 (8):  830-833 .  Abstract ( 475 )   PDF (322KB) ( 2708 )   [Abstract] Objective   To investigate the effect of creatine phosphate on postoperative fatigue of patients with gastric carcinoma after operation．Methods   Nighty cases with gastric carcinoma were randomly divided into control group (n=45) and study group (n=45)．Intravenous drip of phosphocreatine was given after anaesthesia induction in study group，and control group received normal saline instead． After operation, the two group patients were all treated with enteral and parenteral nutrition. General performance was observed in both groups. Fatigue score was measured on the day before the operation，the first，third and fifth day after operation．Body weight, triceps skinfold thickness (TSF), mid upper arm circumference (AMC), plasma total protein (TP), plasma albumin (ALB), prealbumin (PA) were detected at the same time． Results   The total effective rate of the control group and the study group were 73.3% and 95.6%, respectively, the difference was statistically significant (P<0.05). Fatigue scores was significantly higher, postoperative TSF, AMC, TP, ALB, PA of two groups were significantly decreased than preoperative, the difference was statistically significant (P<0.05); the above indexes of treatment group were significantly better than the control group, the difference was statistically significance (P<0.05). Conclusion   Creatine phosphate can alleviate postoperative fatigue, improve the nutritious status and promote early rehabilitation of patients． Related Articles | Metrics

循证医学

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Meta-Analysis of Effect of Continuous Renal Replacement Therapy to Severe Acute Pancreatitis zhe Wang 2014, 42 (8):  834-839 .  Abstract ( 489 )   PDF (693KB) ( 2664 )   [Abstract]  Objective   To assess the effect of continuous renal replacement therapy (CRRT) on severe acute pancreatitis (SAP)．Methods   According to the collaborative search strategy，MEDLINE(1966-2013.9), OVID(1963-2013.9), Chinese Wanfang database(1996-2013.9）,CNKI（1979-2013.9）, the clinical control test database of Cochrane Library and were searched．Related literature，whether Published or not and meeting summary included，were searched by hand．Quality assessment and data extraction were conducted by two independent investigators．Meta-analysis was conducted by RevMan 5.0.Results   A total of 15 reports were identified which met the inclusion criteria，including 652 patients．Compared with control group ,the serum amylase decreased in CRRT group [WMD =-140.76，95%CI（-212.42～-69.11），P=0.0001], inflammatory mediators removed effectively and APACHEⅡ score also decreased in CRRT group [WMD=-3.09，95%CI（-4.37～-1.45，P=0.0002]. The overall survival rate was significantly higher in CRRT group than that in control group[RR=1.35，95%CI（1.19～1.52），P<0.00001].Conclusion   Continuous renal replacement therapy could effectively improve the prognosis of patients with severe acute pancreatitis. Related Articles | Metrics

综述

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Role of Runx2in Breast Cancer Yao TIAN 2014, 42 (8):  840-842 .  Abstract ( 550 )   PDF (288KB) ( 3215 )   【Abstract】  Runx2 is a member of the RUNXX family of transcription factors, as one of the important factors involved in expression and participation in the regulation of mammary specific genes in the mammary gland.In this review, we will focus on the role of Runx2 in breast cancer. Related Articles | Metrics

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Study of Patients’Quality of Life after Different Rectal Surgery Approaches 2014, 42 (8):  843-845 .  Abstract ( 475 )   PDF (301KB) ( 2748 )   [Abstract]   Surgery is the main treatment for rectal cancer, which is one of influencing factors of the patients’quality of life (QoL). Factors affecting QoL in a operation include tumor location, surgical margin, operation approach, appearance of stoma, application of laparoscopy and so forth. Still, now there are lots of arguments on how operations affecting QoL. The fol?lowing is a review about patients’QoL after different rectal surgeries, including high versus lower anterior resection, anterior versus inter sphincteric resection, transanal versus open operation, anal-sparing versus stoma operation, laparoscope versus open surgery. Related Articles | Metrics

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Research of Correlation between Deep Vein Thrombosis and Ankle Fractures Wei Tian 2014, 42 (8):  846-744 .  Abstract ( 507 )   PDF (272KB) ( 2691 )   [Abstract]   Deep vein thrombosis (DVT) is a serious complication of fractures. The incidence of ankle fracture which occurs most frequently intra-articular is200/100000, about 3.9% of all fractures. Domestic and foreign reports of ankle fractures and DVT were allocated and analyzed to review their correlation. Furthermore, different methods of rou?tined thromboprophylaxis was discussed. DVT incident after ankle fractures and the necessity of routined thromboprophy?laxis were reviewed. DVT incidence after ankle fractures was low and routine thromboprophylaxis was not necessary to all ankle fracture patients except the ones who had risk factors of thrombosis which could induce DVT. Related Articles | Metrics