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15 August 2013, Volume 41 Issue 8 Previous Issue    Next Issue
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论著
实验研究
短篇论著
实验研究
短篇论著
新技术交流
临床论丛
短篇与病例报告
抗凝专栏
实验研究
论著
实验研究
论著
短篇论著
论著
实验研究
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论著

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Relation of Isolated Systolic High-Normal Blood Pressure and Coronary Heart Disease in Middle-Aged and Elderly Persons CHEN Mengying ,ZHANGJing ,WANGWei ,liangrong liang rong 2013, 41 (8):  0-746 .  Abstract ( 408 )   PDF (316KB) ( 2689 )   Abstract   Objective   To investigate the relationship between isolated systolic high-normal blood pressure and coronary heart disease (CHD) in a middle-aged and elderly population. Methods  Total of 236 patients over 50 years old and undergone coronary angiography were enrolled and divided into isolated systolic high-normal blood pressure (ISHNBP) group (n=135) and non-ISHNBP (NISHNBP) group (n=101) according to their systolic and diastolic blood pressure levels. All clinical and angiographic data were collected. Results   Compared to the NISHNBP group, systolic blood pressure in ISHNBP group was a bit lower but without statistical significance, while diastolic blood pressure was lower and the resulting pulse pressure was higher, both with statistical significance. Using multivariate Logistic for adjustment of age, gender and other common risk factors of CAS, isolated systolic high-normal blood pressure was associated with CHD (OR=2.67，95%CI:1.50~4.75, P<0.01). The distribution of diseased coronary vessel numbers was more extensive than in the ISHNBP group (P<0.01). Conclusion    In the middle-aged and elderly population studied, isolated systolic high-normal blood pressure is an independent risk factor of CHD. Related Articles | Metrics

实验研究

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Exogenous Hydrogen Sulfide Stimulates the Angiogenesis of Rat Glioblastoma LI Zhanyong 1,KONG Deqiang 1,YANG Zhuo 2,ZhangTao 2 2013, 41 (8):  0-746 .  Abstract ( 405 )   PDF (687KB) ( 2145 )   Objective   To investigate the stimulating effect of exogenous hydrogen sulfide (H2S) on angiogenesis in glioblastoma (GBM) and the role of matrix metalloproteinases-2 (MMP-2) in the process. Methods   20 adult Sprague–Dawley (SD) rats were randomly divided into 2 groups of 10 rats of each, which were glioma group (C6 glioma cell intracerebral implantation) and glioma-H2S group (C6 glioma cell intracerebral implantation and NaHS intraperitoneal injection). The tumor-bearing rat model was established by intracerebral injection of rat C6 glioma cell line, and an intraperitoneal injection of sodium hydrosulfide (NaHS), an exogenous H2S donor was administrated after 1 week of intracerebral injection in glioma-H2S rats. Food and water were freely available during all phases of the experiment. 3 weeks post intracerebral injection, all the rats were decapitated and the brains were removed. HE staining was performed to confirm and observe the tumor as well as the immunohistochemical analysises on CD34 and MMP-2 were carried out, respectively. The microvessel density (MVD) in GBM was represented by CD34 expressing and assessed respectively. Results   HE staining showed that the implanted tumors were predominantly spheroid with clear border and no capsule could be detected. Pseudopalisades and tumor cavity were clearly observed and the neovascular proliferations were also observed in two groups. All these phenomena were more markedly in glioma-H2S group. Immunohistochemical analysis exhibited that the CD34 and MMP-2 expressions were obviously increased after NaHS intraperitoneal injection in glioma-H2S rats (P＜0.001). The MVD of glioma-H2S group was higher than that of glioma group. Conclusion   Exogenous H2S serves as a stimulator of angiogenesis in the development of rat GBM. The tumor microenvironment was involved in the stimulating mechanisms included the promotion of endothelial cell proliferation and the increase of MMP-2 expression. Related Articles | Metrics

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The Effect of Fluvastatin on Ventricular Remodeling and VWF in Heart Failure Rats WANG shi kai1,WANG Haiyan 2,XUE Yanju 3,LIANG Jiangjiu 3 2013, 41 (8):  0-746 .  Abstract ( 343 )   PDF (1045KB) ( 2176 )   Abstract  Objective  To study the effect of fluvastatin on cardiac remodeling and cardiac function , and to investigate the effect of VWF. Method   CHF model was induced by soproterenol via subcutaneous injection and rats were randomly divided into Placebo group and fluvastatin group , Another normal 10 rats served as the control group. Fluvastatin group were treated with fluvastatin (20mg /kg/d), Placebo group and control group were treated with normal saline.After 6 weeks treatment,the structure and function of hearts in different groups were measured by echocardiography ;measured ventricular weight index, the serum levels of VWF and BNP were measured by ELISA; the levels of VWF mRNA in cardiac muscle were measured by RT-PCR. Results  Compared with the control group, LVEDD, LVESD, LVRW, RVRW and VWF mRNA were significant increased in the Placebo group and drug groups, while LVEF and LVFS were significant decreased;Compared with placebo group,LVEDD,LVESD,LVRW,RVRW and VWF mRNA were significant decreased in the drug groups, while LVEF and LVFS were significant increased in the drug groups;The level of VWF were positive correction with the BNP. Conclusion   Fluvastatin could improve the cardiac function and prevent the cardiac remodeling, the mechanisms may be by reducing the levels of VWF and improving endothelial function. Related Articles | Metrics

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The Expression of Metallothionein-1 in Mouse Kidney TIAN Juan 2013, 41 (8):  0-746 .  Abstract ( 334 )   PDF (366KB) ( 2055 )   Objective   To survey the expression and cellular localization of metallothionein-1 (MT-1) in mouse kidney. Methods   To detect the protein of MT-1 in mouse renal cortex and medulla by Western blot. Immunohistochemistry，double-immunofluorescence and confocal laser scanning microscopy were applied to observe the intracellular location of MT-1 in structures of mouse kidney. Results   The expression of MT-1 was detected in cortex and medulla of mouse kidney. The expression of MT-1was mainly in proximal convoluted tubule and distal convoluted tubule of cortical labyrinth, and proximal straight tubule, distal straight tubule and collecting duct of ray medullary and medulla of kidney. The most strongest expression of MT-1 was in proximal tubule, secondary in distal tubule and last in collecting duct. The intracellular location of MT-1 was in intracytoplasm, specific in lysosome, of proximal tubule and distal tubule; whereas was in brush borde of proximal tubule and basilar part of collecting duct. Conclusions    The differdnt of MT-1 expressed in proximal tubule, distal tubule and collecting duct may determine different roles of it in kidney. Related Articles | Metrics

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The changes of growth differentiation factor-15 in the newly diagnosed patients with primary hypertension ZHANG AI JUN 2013, 41 (8):  0-746 .  Abstract ( 376 )   PDF (293KB) ( 1973 )   Related Articles | Metrics

实验研究

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Changes of Cytokine Expression in the Hippocampus of Aβ1-42-Induced Alzheimer’s Disease Rat Model ZHANG Xuemei 1,KE Kaifu 2,FANG Xiaoxia 1,QIU Yihua 1,PENG Yuping 1 2013, 41 (8):  0-746 .  Abstract ( 443 )   PDF (605KB) ( 2202 )   Objective   To explore changes of pro- and anti-inflammatory cytokines expression in the hippocampus of Aβ1-42- induced AD rat model. Methods   Sprague Dawley rats (200 ± 20 g) were injected Aβ1-42 , the escape latency was evaluated by Morris water maze. Lesion of neurons within the hippocampus CA1 area was detected by Nissl staining. The levels of amyloid precursor protein (APP) and protein phosphatase 2A (PP2A) in hippocampus were measured by means of Western blot analyses. Real-time PCR was employed to examine the expressions of pro- inflammatory cytokines, including interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and anti-inflammatory cytokines including IL-4, IL-10, transforming growth factor-β (TGF-β). Results   Rats subjected to Aβ1-42 injection within bilateral hippocampus confirmed the success to make AD rat models, which led to reduction of the ability of learning and memory; Nissl staining showed pyramidal cells disturbed, neurons decreased significantly. the treatment with Aβ1-42 notably increased expression of APP but decreased PP2A expression level. There was evidently up-regulation in the expressions of pro-inflammatory mediator mRNAs in AD rats, including IL-1β, TNF-α and IFN-γ. Contrarily, the expressions of anti-inflammatory cytokine mRNAs, including IL-4, IL-10 and TGF-β, were significantly down-regulated relative to the control rats. Conclusions   Aβ1-42 injection in bilateral rat hippocampus induces AD model. Neuroinflammation resulting from pro- and anti-inflammatory unbalance is involved in pathogenesis of AD. Related Articles | Metrics

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Correlative study on ADC value and the grading in hepatocellular carcinoma LIXu-Bin ,HOU YAN ,FENG ,YE ZHAO XIANG 2013, 41 (8):  0-746 .  Abstract ( 410 )   PDF (239KB) ( 2068 )   Objective   To explore the value of apparent diffusion coefficient (ADC) in the grading of hepatocellular carcinoma (HCC). Methods   MR images of 92 patients with HCC proved by histology were reviewed. All patients were performed plain and enhanced MRI and DWI. The ADC values were calculated, the difference of the ADC values of HCC with different grade and the relationship of ADC value and the differentiation were evaluated, respectively. Results    The mean ADC value of the poorly-differentiated HCC (0.9 ± 0.13×10-3 mm2/s) was lower than those of the well-differentiated HCCs (1.263±0.388×10-3mm2/s) (p< 0.05) and moderately-differentiated HCC (1.072±0.227×10-3mm2/s) (p<0.05). There was a negative correlation between the differentiation and the ADC value of the HCC (r = -0.404, p=0.0001). Conclusion   ADC value plays an important role in the preoperative grading of HCC. Related Articles | Metrics

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Neonatal methylmalonic aciduria: a report of 7cases and review of 12 cases of China ZHANGYING ,GUO Jing ,WANG Dan 2013, 41 (8):  0-746 .  Abstract ( 325 )   PDF (374KB) ( 2192 )   Objective   To acquire more knowledge about neonatal and prevent and treat it better by investigating the clinical feature and therapeutic experience about it. Methods    7 cases with Neonatal methylmalonic aciduria who were seen in Tianjin Children’s Hospital from Jan 2009 to Oct 2012 are reported in this paper and 12 cases reported previously in recent seven years in China were reviewed. Result   Totally 19 cases were summarized, 11 males and 8 females. Ages of onset were from 24 hours to 27 days. The main clinical manifestations were feeding difficulty, dyspnoea, twitch, et al. Routine laboratory examinations were metabolic acidosis, hyperammoniemia, et al. GC-MS analysis of urinary organic acid declared that 19 cases’ Urinary methylmalonic acid concentration were significantly higher. 10 cases died, 6 cases significant hypoevolutism during 2 to 8 months follow-up visits, 3 cases lost because of giving up treatment. Conclusion   Neonatal methylmalonic aciduria has nonspecific symptoms early such as feeding difficulty, et al. Routine examinations were short of specificity. Fatality rate and disability rate were very higher. In order to diagnose and treat early, we should detect the amino acids of blood and urine of suspected cases in time. So then we can improve the prognosis. Related Articles | Metrics

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The Characteristics and Treatment of Major Depression in the Rehabilitation Phase of Traumatic Brain Injury LUO LAN LAN ,CAIYAN ,JIANG RONG CAI ,CHENXIN 2013, 41 (8):  0-746 .  Abstract ( 370 )   PDF (238KB) ( 2242 )   Objective   This study was designed to investigate the characteristics of clinical diagnosis and treatment of major depression in the rehabilitation phase of traumatic brain injury (TBI). Methods   32 patients, suffered from TBI and discharged with major depression, were recruited in this study. Their TBI characteristics, clinical manifestation and management of major depression were comprehensively analyzed. The therapeutic effects of different treating regimen were further compared as well. Results   10 cases presented with mild-to-moderate (31.25%); 18 cases with moderate (56.25%); and 4 cases with severe depression (12.5%). Their clinical manifestations are easy fatigue (22 cases), irritability (21 cases), hypologia (20 cases), insomnia (15 cases), and concentration deficits (14 cases). 15 patients suffered from reduced hormone level (12 cases with hypocortisolism, 10 with hypothyroidism, and 3 with hypogonadotropinism and hypoestrogenism). After supportive psychotherapy, hormone replacement and antidepressant treatment, 68.75% of depressive patients were in complete remission, 28.13% in partial remission, 3.13% with no obvious improvement. Conclusion    Major depression is a common complication in the rehabilitation phase of TBI, and post-traumatic hypopituitarism might be a major contribution factor. Appropriate and prompt treatment could significantly improve or even cure the major depression in TBI patients. Related Articles | Metrics

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Pathogens and Clinical features of Catheter-related Bloodstream Infection LIUXue-Hua ,wu qi 2013, 41 (8):  0-746 .  Abstract ( 368 )   PDF (254KB) ( 2170 )   Abstract   Objective     To explore pathogenic etiology and clinical features of catheter-related bloodstream infection(CRBSI), and to provide basis for the empiric therapy of CRBSI．Methods  All patients who had the definite diagnosis of CRBSI were investigated from Feb,2011 to Oct, 2012 in Tianjin People,s Hospital.The pathogens, clinical features, inflammatory markers，and prognosis were analyzed retrospectively．Result  There were 91 pathogenic strains isolated from 91 patients who were diagnosed as CRBSI．In which 31 strains (34.1% ) were fungi, 31 strains (34.1% ) were gram-negative bacilli，29 strains （31.9%）were gram-positive cocci. There was no difference in the constituent ratio between three groups (P>0.05). CRBSI clinical features caused by fungi and gram-negative bacilli present hyperpyrexia with shiver. Low or moderate fever without shiver were more frequent in gram-positive cocci group. Inflammatory markers level in Three groups of pathogens rised, but were more higher in fungi and gram-negative bacilli group.mortality rate was more higher in fungi group（p< 0.05）.Conclusion    Fungi, gram-negative bacilli and gram-positive cocci are equally pathogenic strains of CRBSl. Investigation of pathogens of CRBSI should be strengthened and empiric antibiotic therapy based on clinical features should be given early to reduce mortality rate. Related Articles | Metrics

新技术交流

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Screening of licorzincum oral ulcer film formulation by orthogonal design sun lei ,CAI LI JUAN 2013, 41 (8):  0-746 .  Abstract ( 364 )   PDF (317KB) ( 1923 )   = Related Articles | Metrics

临床论丛

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The molecular mechanism study of distribution of Neurotrophic factors in the distal of rectum with congenital Imperforate anus. zhanjianghua ,HU BO ,ZHAO LIN SHENG ,LUOXirong ,NIU JUN ,ZHU QIAN YI ,LIN HONG 2013, 41 (8):  0-746 .  Abstract ( 353 )   PDF (259KB) ( 1903 )     Related Articles | Metrics

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Observation of Audiology in severe Obstruc-tive sleep apnea- hypopnea syndrome CHEN CHEN 2013, 41 (8):  0-746 .  Abstract ( 335 )   PDF (240KB) ( 2023 )     Related Articles | Metrics

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The comparison between carbon nanoparticles suspension and methylene blue used in sentinel lymph node biopsy for early and middle stage of breast cancer SUN XUAN 1,GE JIE 1,CAO Xuchen 2 2013, 41 (8):  0-746 .  Abstract ( 396 )   PDF (278KB) ( 2190 )     Related Articles | Metrics

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The observation of the immune effect to the 203 newborns with simply HBeAg positive YOU WEI 2013, 41 (8):  0-746 .  Abstract ( 312 )   PDF (246KB) ( 2058 )     Related Articles | Metrics

短篇与病例报告

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Clinical pathology review of primary tracheobronchial amyloidos 2013, 41 (8):  0-746 .  Abstract ( 328 )   PDF (286KB) ( 2034 )     Related Articles | Metrics

抗凝专栏

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Analysis of the different perioperative anticoagulant effects for the elders with intertrochanteric fracture Bin LIU 2 2013, 41 (8):  0-746 .  Abstract ( 399 )   PDF (310KB) ( 1963 )   Objective    In order to compare the perioperative anticoagulant effect of Rlvaroxaban with that of low molecular heparin calcium for elders with intertrochanteric fracture. Method    We have retrospectively analyzed the anticoagulant effect of 122 elders with intertrochanteric fracture, who were in hospital between March 2008 and January 2012 and have all underwent a surgery. Of the patients 62 received the treatment with low molecular heparin calcium, and 60 were treated with Rlvaroxaban. We have compared the mean operative blood loss, mean wound healing time and mean wound dressing time, and evaluated the two groups’ rate of deep venous thrombosis. Result   There is no significance in mean operative blood loss, mean wound healing time and mean wound dressing time between two groups（P＞0.05）. There are 6 cases formed thrombosis in group with low molecular heparin calcium and 4 cases formed thrombosis in group with Rlvaroxaban, 2 cases of 6 patients formed thrombosis after discharge. There is no significance in the two groups’ rate of deep venous thrombosis（P＞0.05）.But subcutaneous ecchymosis has formed in 4 cases and the count of platelet reduced in one case in group with low molecular heparin calcium, and there is no case with complications in group with Rlvaroxaban. Conclusion    Rlvaroxaban and low molecular heparin calcium both have a good perioperative anticoagulant effect for elders with intertrochanteric fracture. But there is low patient compliance for hypodermic injection of low molecular heparin calcium, and some cases subcutaneous ecchymosis have formed and the count of platelet reduced in some cases, patients have a high risk of deep venous thrombosis because of no treatment after discharge. There is a high patient compliance for oral therapy of Rlvaroxaban, and patients can also continue taking after discharge, and it is an anticoagulant drug with the advantage of excellent effect and convenient taking. Related Articles | Metrics

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Preparation and Characterization of Rat Adipose-Derived Stem Cells and XIAP Modified Adipose-Derived Stem Cells BAI Yaobang ,LI bo ,CHEN Qingliang ,JIANG Nan ,CHEN Xiaobo 2013, 41 (8):  0-746 .  Abstract ( 428 )   PDF (357KB) ( 1999 )   Objective   To isolate and cultivate rat ADSCs in vitro, and to identify its differentiation potential. Modification of ADSC and test test the antiapoptotic ability after XIAP modification. Methods   ADSCs were isolated from rat groin fat pads by CollagenaseⅠdigestion under sterile condition. ADSCs were passaged and amplified with 10% FBS DMEM. The multi-differentiation potential of ADSCs were verified by cultivated with differentiation medium. Electrotransduction of XIAP expression plasmid into stem cells was done followed with normal procedure. The antiapoptotic effection of XIAP transduction was verified by the downregulation of caspase activation.Results   In vitro cultured ADSCs were mainly spindle-shaped and whirlpool-shaped arranged. ADSCs were positive expression of CD29、CD44、CD105 and CD90, ADSCs can differentiate into lipocytes,chondrocyte and osteoblasts under certain conditions. The over-expression of XIAP in ADSCs can enhance the ADSCs antiapoptotic ability, improve its tolerance of ischemia and hypoxia.Conclusion   ADSCs were isolated from rat subcutaneous fat pads and were easily cultivated, passaged and amplified. ADSCs were highly effective modified by electrotransduction, and differentiated into osteoblasts、chondrocyte and adipocytes under certain conditions. ADSC is a better resource for being used in cell therapy and tissue engineering. Related Articles | Metrics

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The expression and clinical significance of HBD-2 in gastric mucosa of H. pylori associated gastric MALT lymphoma patients ZHAI Haoyu 1,ZHANG Zhi guang2,JIANG Kui 3,LI Man 2 2013, 41 (8):  0-746 .  Abstract ( 355 )   PDF (458KB) ( 2066 )   [Abstract]  Objective  To investigate the expression of humanβ-defensin 2 (HBD-2) in gastric mucosa of Helicobacter pylori (H. pylori) associated gastric mucosa-associated lymphoid tissue (MALT) lymphoma, and the role of HBD-2 in gastric MALT lymphoma. Methods   Forty gastric mucosa specimens from patients with H. pylori associated gastric MALT lymphoma were collected. And 36 gastric mucosa specimens from chronic superficial gastritis without H. pylori infection were included as control group. The expression of HBD-2 was detected by immunohistochemistry staining. Results   The expression of HBD-2 was significantly higher in H. pylori associated gastric MALT lymphoma than that of control group. (P＜0.01). The expression of HBD-2 was significantly decreased after the eradication of H. pylori (P＜ 0.01). The expression of HBD- 2 was significantly higher in H. pylori associated gastric MALT lymphoma than that of lymphoma cells (P＜ 0.01).There was no expression of HBD-2 in lymphoma cells. Conclusion  HBD-2 is possibly involved in the pathogenesis of H.pylori associated gastric MALT lymphoma. But whether it has anti-tumor effect is not clear. Related Articles | Metrics

实验研究

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Baicalin promotes neuronal differentiation of neural stem/progenitor cells through downregulating p-stat3 CUI Meng ,FENG Shiqing ,FAN Ningjian ,JIA Jun ,ZHOU Xianhu 2013, 41 (8):  0-746 .  Abstract ( 431 )   PDF (300KB) ( 2096 )   [Abstract]  Objective  To observe the role of baicalin on the expression of phosphorylated protein of signal transducers and activators of transcription signaling proteins (STATs) during the process that neural stem cells (NSCs) differentiating into neurons. Methods  NSCs were isolated from the embryonic cerebral cortex of the 14-15-day pregnant SD rats, which were cultured and passaged in vitro. The 3rd generation of NSCs was used in the experiment. NSCs were randomized into natural differentiation control group, three different doses of baicalin groups (7.5 μmol/L, 15 μmol/L and 30 μmol/L), leukemia inhibitory factor (LIF) + basic fibroblast growth factor (bFGF) group and baicalin + LIF + bFGF group. After 6 d culture in vitro, the immunohistochemical method was used to observe the expressions of microtubule-associated protein 2(MAP-2) and glial fibrillary acidic protein (GFAP) in different groups. The expression levels of phosphorylation protein of STAT3 in NSCs were detected by Western blotting method after 2 h and 6 d of culture. Results The expression of MAP-2 in NSCs was increased by baicalin, but the expression of GFAP in NSCs decreased. The expression of GFAP in NSCs was enhanced in LIF+bFGF group, which was inhibited by baicalin + LIF + bFGF. The phosphorylation level of STAT3 in NSCs was downregulated by baicalin, but the phosphorylation level of STAT3 was upregulated in LIF+ bFGF group. The upregulated phosphorylation level of STAT3 was inhibited in baicalin + LIF + bFGF group(P＜0.05). Conclusion   Baicalin can induce NSCs to differentiate into neurons, which may be caused by the downregulation of the phosphorylation level of STAT3 in NSCs. Related Articles | Metrics

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The Influence of CagA on The Differentiation of Helper T Lymphocytes in Chronic Gastritis Zhao-Lan XIE 2013, 41 (8):  0-746 .  Abstract ( 333 )   PDF (403KB) ( 2056 )   Objective   To evaluate the influence of cytotoxin associated gene A to the differentiation of helper T lymphocytes in chronic gastritis. Methods   Eighty patients with chronic gastritis were in roll, the serum antibody against CagA was detected through ELISA. We divided the patients into CagA-positive and CagA-negative groups. Pathologic changes in gastric mucus were respectively analyzed. In addition, the expressions of nuclear transcriptive factors in gastric mucus reflecting the differentiation of Th subsets including TBX21、GATA-3、FoxP3 and Rorγt were further detected. Results   The gastric inflammation in CagA-positive group was more serious than that in CagA- negative one. However, the statistical difference of Hp density between them was not observed. The expressions of GATA-3 and FoxP3 were much higher while those of TBX21 and Rorγt much lower in CagA-positive group than that in CagA-negative one. Conclusions   Although the inflammation in gastric mucus of CagA-positive patients was relatively more serious, Hp can not be effectively eliminated，which may relate to the differentiation of Th0 into Th2/Treg cell. Related Articles | Metrics

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The Association between Matrix Metalloproteinase-10 and Atherosclerosis in Maintenance Hemodialysis Patients SUNGui-Jiang 2013, 41 (8):  0-746 .  Abstract ( 298 )   PDF (251KB) ( 1952 )   Objective   To explore whether there is an association between matrix metalloproteinase-10 (MMP-10) and atherosclerosis in maintenance hemodialysis patients. Method   All individuals were sampled randomly from our hospital, 60 patients from the center of blood purification, 30 healthy subjects from medical examination center whose carotid intima media thickness (cIMT) are normal. All indexes are taken to comparison between 2 groups. We analysis the association between MMP-10 and other indexes in experiment group, and divide these patients into 4 groups accord to MMP-10 and take the number of patients with increased cIMT and plague to comparison between 4 groups. Besides, we also take increased cIMT and plague into Logistic stepwise regression analysis. Results    1)There is significant difference of systolic pressure, diastolic pressure, Glu, Alb, BUN, Cr, HDLc, MMP-10, TIMP-1, cIMT between two groups. 2) There is positive association between serum MMP-10 and age, hsCRP, cIMT, PROCAM, but a negative association between serum MMP-10 and Alb (P＜0.05). We don’t find significant correlation between MMP-10 and TIMP-1. cIMT is positively and significantly associated with age, PROCAM, MMP-10(P＜0.05). We don’t find any significant correlation between TIMP-1 and other items. 3) The patients with increased cIMT (χ2=8.938，P=0.030) and plague (χ2=8.782，P=0.032) are increased with a higher concentration. 4) Logistic stepwise regression analysis reveals the increased LDL-c/HDL-c may be a risk factor of the increased cIMT in maintenance hemodialysis patients.Overweight and increased cIMT may be risk factors of the plague(P＜0.05) in maintenance hemodialysis patients. Conclusion    MMP-10 is positively and significantly associated with cIMT and plague, but MMP-10 is not the risk factor of them. Related Articles | Metrics

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Effect of B-Vitamin Therapy on Neurological Dysfunction of Brain Infarction in Patients with Hyperhomocysteinemia ZHUJu 1,ZHANG ZHECHENG 1,ZHANG JING 1,WangSu-Hong 1,WangYu 2 2013, 41 (8):  0-746 .  Abstract ( 509 )   PDF (350KB) ( 2271 )   Objective   To investigate whether Vitamin B complex supplements would reduce stroke-related disability in hyperhomocysteinemia patients with recent ischemic stroke. Methods   1000 brain infarction patients with hyperhomocysteinemia were assigned to receive either a daily dose of Vitamin B complex (Treatment group, n=500) or not (Control group, n=500) on the base of conventional secondary prevention medications for a period of 2 years. Neurological dysfunction was assessed by National Institutes of Health Stroke Scale (NIHSS) and stroke disability was evaluated by Barthel Index (BI) score. High performance liquid chromatographic method with fluorescence detection was used for the determination of total plasma homocysteine levels. After 2 years of follow-up, the patients in the treatment group, whose tHcy level was reduced by 3-μmol/L or more, was defined as the treatment subgroup. Results  Homocysteine levels were significantly reduced in the treatment group. A lower NIHSS Scale was obtained at 12m, 18m, 24m in the treatment group compared to that in the control group (P<0.05), there were no statistical significance difference in the two groups at other time points (P>0.05). For the BI score, there were no statistical significance difference in the two groups at any time points (P>0.05). After 2 years of Vitamin B complex supplementation, the treatment subgroup was associated with a lower NIHSS Scale and higher BI Scale compared to that in the control group (P<0.05). Conclusion   Lowering of homocysteine may have bneficial effects on neurological functional outcome at 1-2 year after stroke onset. Who have significant reduction of homocysteine level, may be most likely to benefit from treatment with B vitamins. Related Articles | Metrics

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Effects of VEGF-C, VEGFR-3 and LYVE-1 on Lymphangiogenesis and Prognosis in Gastric Cancer LIU Xinlan 1,xinlan Liu2,zhilan-Ma 2 2013, 41 (8):  0-746 .  Abstract ( 422 )   PDF (572KB) ( 2000 )   [Abstract] Objective   To study the expression of Vascular endothelial growth factor C(Vegf-C),vascular endothelial growth factor receptor-3(VegfR-3),lymphatic vessel hyaluronan receptor-1(lyve-1) in gastric cancer, To analyze the possible relationship between the expression of Vegf-C,VegfR-3 and lyve-1 with the lymphangiogenesis and prognosis. Methods  Application of Tissue Microarray Technology, The immunohistochemical technique SP was used to detect the Vegf-C, VegfR-3 and lyve-1 in 125 gastric cancer specimens and 96 normal tissues and 20 patients with benign gastric lesion．The lymphatic vessel density (LVD) was assayed by Podoplanin marks . Results    The positive rate of Vegf-C, VegfR-3 and lyve-1 in gastric cancer tissue were 62.4%,56.0% and 58.4%, higher than those in adjacent normal tissue and in gastric lesion tissue,which were 10.4%,12.5%,9.4% and 20%,30%,25% (P<0.05).The LVD score in gastric cancer was significantly higher than in adjacent normal tissues or benign gastric lesion (P<0.01)(2.98±0.81/4.22±1.09vs1.82±0.63or0.89±0.45),the LVD score in peri-tumoral was higher than in intra-tumoral(P<0.01).There is a positive relationship between expression of Vegf-C,VegfR-3 and lyve-1 with LVD (P<0.05). The positive expression of three indexes were the risk factors of lymph node metastasis and distant metastasis(P<0.05). The negative expression of three indexes also had a long 5 year survival rates(P<0.05). Conclusion   Vegf-C, VegfR-3 and lyve-1 protein positively highly expressed in gastric cancer tissue,which were risk factors influence the prognosis of gastric cancer.The positive expression of three indexes can be used to predict the prognosis and lymphangiogenesis of gastric cancer. Related Articles | Metrics

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Effects of Cytokine-Induced Killer Cells on Different Tumor Cell Lines in Vitro LI Xinling ,WANG Zhishuang ,SHAO Xiaofeng ,REN Feng 2013, 41 (8):  0-746 .  Abstract ( 427 )   PDF (366KB) ( 2107 )   Objective   To investigate the proliferation, immune phenotype and cytotoxicity on different cell lines of CIK cells collected from healthy donors. Methods   Peripheral blood mononuclear cell(PBMC) from healthy donors were induced to become CIK cells by adding cytokines including rhIL-2, rhIFN-γ and CD3 McAb. Proliferation of CIK cells was tested by blood cell recording board. The CIK cells were analyzed on different time points by FACS; The cytotoxicity of CIK cells against different tumor cell lines such as K562, BJAB, A549, MCF-7, HepG2 in vitro by MTT assays on day 13. Results  CIK cells quickly proliferated from day 5, and expanded by 182-fold after 20 days’ culture. The immunophenotypes of CD3+, CD3+CD8+, CD3+CD56+ were (97.83±1.03) %, (77.12±1.60) % and (27.58±2.02) %. The percentages of CD3+, CD3+CD8+, CD3+CD56+ increased noticeably (P<0.01). According to the effector-target ratio of 40:1, the activity of CIK cells against tumor cells K562, BJAB, A549, MCF-7, HepG2 were (88.89±7.22) %, (75.42±9.52) %, (63.19±5.67) %, (43.53±5.67) % and (42.63±7.69) %. The experiment showed that CIK cells possessed higher antitumor cytotoxic activity. Conclusion   CIK cells can be largely capacity cultured by adding cytokines in vitro. CIK cells was a highly efficient cytotoxic cells against tumors, and had clinical application potentials. Related Articles | Metrics

实验研究

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High density culture of embryoid bodies enhanced cardiac differentiation of murine embryonic stem cells CHEN Ming 1,BI Linlin 2,ZHAO Fang 1,WANG Zhiquan 1,GAN Xuedong 1,WANG Yanggan 1 2013, 41 (8):  0-746 .  Abstract ( 458 )   PDF (540KB) ( 2045 )   [Abstract] Objective To investigate the role of different culture densities of embryoid bodies (EBs) in cardiac dif?ferentiation of mouse embryonic stem cells (ESCs). Methods  The mouse ESCs were cultured in hanging drops for 3 days,followed by another 2 days for suspension culture to form EBs. Suspended EBs of different densities (60 or 120 EBs/60 mm tissue culture dish) were transferred onto tissue culture plates. The cardiac specific troponin T (TnT) was detected by immunocytochemistry. The percentage of beating EBs was calculated at different time points. The mRNA expression of cardiac specific transcriptional factors Nkx2.5, GATA4 and cardiac specific proteins β-MHC and ANF were detected by RT-PCR. The protein expressions of TnT and p38 were detected by Western-blot assay. Results    Spontaneously beating EBs were positively stained with TnT. There were significantly higher percentage of beating EBs, higher gene expression levels of Nkx2.5,GATA4, β-MHC and ANF and higher protein expression of TnT in high density culture group than those of low density culture group (P＜ 0.05). Furthermore, there was significantly higher activity of p38 pathway in high density culture group than that of low density culture group. And the percentages of beating EBs and TnT protein expression were decreased by p38 pathway inhibitor SB203580. Conclusion   The culture density of EBs is important in regulating the cardiac differentiation of ESCs. The high cell-density density culture of EBs enhances the cardiac differentiation of ESCs, which might be mediated by p38 signaling pathway. Related Articles | Metrics

论著

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To Study the value of 256 slice spiral CT angiography in internal thoracic artery CHEN Weibin 2013, 41 (8):  0-746 .  Abstract ( 412 )   PDF (343KB) ( 2065 )   Abstract  Objective   To study the value of 256 slice spiral CT in internal thoracic artery. Methods   retrospectively analysis the angiographic images of 107 patients who were suspected of thoracic and abdominal aortic disease, through observed the original image and maximum intensity projection ( MIP ) image and volume rendering ( VR ) image, preliminary observed the internal thoracic artery running rule and its branch on both sides, each side of the internal thoracic artery were divided into 3 sections, then measured lumen and vessel length and its branches of three segmental vascular of the internal thoracic artery, measured the lumen of diaphragmatic artery diameter, measured the distance between internal thoracic artery and each border of sternum. Results  107 cases of patients with both sides of the internal thoracic artery and its branches diaphragmatic arteries can be shown, all of the selected cases of internal thoracic artery have arisen from both sides of the subclavian artery, downward along the inner side of costal cartilage, to the level of the sixth intercostal space were divided into superior epigastric artery and diaphragmatic arteries, two side intrathoracic artery proximal to the vascular lumen diameter , left were ( 2.52± 0.38 ) mm, right were ( 2.49 ±0.27) mm, the middle section , left were ( 2.32 ±0.19) mm, right were ( 2.30 ±0.22) mm, the distal left were ( 2.02± 0.17 ) mm, right were ( 2.13 ±0.12) mm, between the three department there were statistics significance ( p<0.05 ), between each side of the corresponding segment there were not statistical significance ( p>0.05 ). The length of left internal thoracic artery average was ( 22.33± 2.10 ) cm, the right internal thoracic artery average was ( 20.96± 1.99 ) cm, there were significant difference ( p<0.05 ). Two sides of the diameter of diaphragmatic arteries: the right were (1.91 ±0.22) mm, left were ( 1.89 ±0.11) mm, there were not significant difference ( p>0.05 ). On both sides the distance of the internal thoracic artery to the sternal edge: left were ( 2.13± 0.17 ) cm, right were ( 2.07 ±0.26),there were not significant difference ( p>0.05 ). Conclusion   the application of 256 slice spiral CT angiography can clearly show the internal thoracic artery running rule and anatomical features, clinical diagnosis and treatment for diseases related to provide a relatively important information. Related Articles | Metrics

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The Clinical Significance of Ghrelin Level in Hp Related Upper Gastrointestinal Diseases ZHANGYing 2013, 41 (8):  0-746 .  Abstract ( 384 )   PDF (277KB) ( 2003 )   Objective  To discuss the impact and clinical significance of HP elimination on ghrelin, by detect the difference of serum ghrelin level and ghrelin express in gastric mucosa before and after HP elimination on upper gastrointestinal disease patients. Method    Determine serum ghrelin level before and after HP elimination with ELISA. Determine ghrelin express in gastric mucosa with RT-PCR. Analysis results with statistical method. Result   Serum ghrelin level in HP infected chronic superficial gastritis group, atrophic gastritis group, peptic ulcer group and gastric cancer group are 26.08±9.14μg/L, 18.59±8.19μg/L, 35.42±9.87μg/L and 18.33±6.88μg/L. Ghrelin express in gastric mucosa are 0.86±0.26, 0.75±0.22, 1.38±0.29 and 0.21±0.09. In chronic superficial gastritis group, both serum ghrelin level and ghrelin express in gastric mucosa were found risen to 29.35±8.83μg/L and 1.10±0.32（P<0.01） 4 weeks after HP elimination therapy. In peptic ulcer group, both serum ghrelin level and ghrelin express in gastric mucosa were found decreased to 30.73±6.50μg/L and 1.09±0.24（P<0.01）. In atrophic gastritis group, significant ghrelin changes were not found before and after HP elimination. Serum PGI/PGII and serum ghrelin were found positively related in chronic superficial gastritis group, atrophic gastritis group and gastric cancer group, r=0.668（P<0.01）. Conclusion  HP infection and HP eliminate rained influence on ghrelin level in patients with HP related upper gastrointestinal diseases. The changes of ghrelin level related on pepsinogen I/II. Ghrelin could be used as one of the indexes of gastric mucosa degree of diagnostic and prognostic evaluation. Related Articles | Metrics

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The Correlation of T peak-T end Interval and Ventricular Arrhythmia in Patients with Acute Myocardial Infarction JIANG Xiangming 1,ZHAO Hongmin 1,JI Zhenguo 1,LIU Gang 2,LIU Lina 3 2013, 41 (8):  0-746 .  Abstract ( 415 )   PDF (346KB) ( 2085 )   [Abstract]  Objective  To investigate the relationship of T peak-T end (Tp-Te) interval and Tp-Te interval dispersion (Tp-Ted) in different periods of myocardial ischemia in patients with acute myocardial infarction (AMI), and to assess the clinical significance of Tp-Te and Tp-Ted for prediction of the ventricular arrhythmia (VA). Methods   A total of 80 patients with AMI were enrolled in the study. The sizes and changes of Tp-Te and Tp-Ted were observed during the acute phase and recovery phase in patients. The differences of Tp-Te and Tp-Ted were compared between ventricular tachycardia group (A group), ventricular premature beats group (B group) and non- ventricular arrhythmia group (C group). Results   The values of Tp-Te and Tp-Ted were obviously longer in acute period [(125.22±17.70) ms and (54.76±13.26) ms] than those in recovery period[ (113.84±17.37) ms and (42.06±13.95)ms] (P＜ 0.01). The values of Tp-Te and Tp-Ted were significantly longer in A group[ (134.82±19.56) ms and (62.00±15.19) ms] than those in B [(122.94±15.09) ms and (54.09±10.56)ms ]and C group [(110.09±15.21) ms and (45.27±9.85) ms]. The values were higher in B group than those of C group. Conclusion   The Tp-Te interval and Tp-Ted prolongated in acute phase than those of recovery phase in patients with AMI. TpTe interval and Tp-Ted can be used as an important index to predict VA in patients with AMI. Related Articles | Metrics