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15 July 2013, Volume 41 Issue 7 Previous Issue    Next Issue
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实验研究
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新技术交流
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实验研究
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短篇论著
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实验研究
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循证医学
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临床论丛
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实验研究

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Effects of Monosialoganglioside on the Expression of Fas in Hippocampus of Cerebral Ischemia-Reperfusion Rats DU Jiang 2013, (7):  0-646 .  Abstract ( 868 )   PDF (2170KB) ( 2502 )   0bjective   To study the expression of Fas in hippocampus of cerebral ischemia-reperfusion rat after monosialoganglioside was injected. Methods   72 SD rats were randomly divided into the sham group, ischemia-reperfusion group(I/R), monosialoganglioside group(GM1) at different time points (6h, 12h, 24h, 3d) after reperfusion on average. The expression of Fas in hippocampus of all groups was examined by immunochemistry and western blot methods. Result   There is a little of Fas positive expression in hippocampus of the rats in sham group, and the positive expression of Fas was increased significantly after reperfusion (P< 0.05), to the peak at 3d, compared with the rats of the I/R group at the same time point, the positive expression of Fas decreased significantly in GM1 group (P< 0.05) ; and the same result was obtain by western blot method. Conclusion   one of the mechanisms for monosialoganglioside participate the pathogenesis of cerebral ischemia-reperfusion injury might be downregulate the expression of Fas. Related Articles | Metrics

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Relationship of Adiponectin and Insulin Resistance in Patients with Chronic Kidney Disease YU Yan 2013, (7):  0-646 .  Abstract ( 579 )   PDF (2072KB) ( 2187 )   [Abstract]   Objective   To investigate the changes of serum and urinary adiponectin (ADPN) levels and insulin resistance (IR) states in patients with chronic kidney disease (CKD) and to explore their relationship. Methods   487 patients with CKD stages 2-5 and 30 healthy subjects served as control group were enrolled in the study. ADPN levels in serum and urine samples were examined by ELISA and fasting insulin by radioimmunoassay. Blood routine test, liver and kidney functions, blood glucose, serum lipids, 24h urinary protein excretion and endogenous creatinine clearance rate were observed, and body mass index calculated. The differences of ADPN levels in serum and urine samples and homeostasis model assessment for insulin resistance (Homa-IR) were compared among the groups. Results   The serum and urine ADPN levels and Homa-IR in CKD patients were higher than those of the control group (P<0.05) and the worse of renal function, the higher of ADPN and Homa-IR levels (P<0.05). The CKD stages 3-5 patients with high Homa-IR had higher serum ADPN levels. The ADPN levels and Homa-IR were positively related to lipid parameters and 24h urinary protein, and negatively correlated with hemoglobin and serum albumin (P<0.05). Conclusion   CKD patients had higher ADPN level and more significant IR. The ADPN and IR were also correlated with serum lipids, hemoglobin, albumin and urinary protein. Dynamic monitor of ADPN level may have clinical significance in judging metabolic disorders in CKD patients. Related Articles | Metrics

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Effects of Folic Acid Supplement on Subjects with Different Methylenetetra-Hydrofolate Reductase C677T Genotypes ZHANG Shuang 2013, (7):  0-646 .  Abstract ( 613 )   PDF (2851KB) ( 2887 )   [Abstract]  Objective  To evaluate the effect of folic acid supplement in different 5,10-methylenetetrahydrofolate reductase (MTHFR) genotype. Methods    113 healthy women from Tianjin were divided into CC(24), CT(58), TT(31) groups according their MTHFR C677T genotype, then in each group subjects were randomly divided into intervention (400 μg/d folic acid supplement) and control(usual diet) groups. Plasma folate, red blood cell (RBC) folate, plasma Hcy concentration were measured at baseline and two months of intervention. Results   The plasma folate was lower (P = 0.003, P = 0.015), and the plasma Hcy was higher (P <0.001, P <0.001) in the TT genotype than that in the CC/CT genotype. After two months of intervention, the plasma folate, RBC folate concentration increased while the plasma Hcy concentration decreased in all three intervention groups. Although the plasma Hcy concentration decreased most in TT genotype, the final average concentration is still larger than 10umol/L (10.09 ± 1.30umol/L). Logistic regression analysis showed that the MTHFR CT, TT genotype was a risk factor of high Hcy concentration, OR=8.078, 95%CI: 1.929-33.834; folic acid supplementation can reduce the risk of high Hcy concentration, OR =0.465, 95%CI: 0.189-1.146. Conclusion   Folic acid supplement can significantly increase plasma folate and red cell folate concentration, and reduce plasma Hcy concentration in all MTHFR genotypes. However, women with TT genotype might need to take longer or higher dose of folic acid. Related Articles | Metrics

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Influence of the Application of Low-Calcium Dialysate in Combination with Calcitriol in Serum Calcium-Phosphorus Levels JIAOChun-Hong 1,GAO Li 1,ZHOU Jie 1,ZHANG Xin 1,SUN Tao 1,3 2013, (7):  0-646 .  Abstract ( 564 )   PDF (2847KB) ( 2396 )   [Abstract]    Objective   To observe the changes of serum calcium, phosphorus, calcium-phosphorus product and parathyroid hormone（PTH） levels after treatment with different concentrations of calcium dialysate in combination with calcitriol. Methods    Thirty-six patients on maintenance hemodialysis were randomly divided into Dca1.25 group and Dca1.5 group, 18 cases in each group. Patients took different doses of calcitriol on the different values of PTH basis. Changes of serum calcium, phosphorus, calcium-phosphorus product， PTH and bone alkaline phosphatase (BAP) levels were recorded respectively on four time points from prior treatment and 1, 3 and 6 months of therapy. The levels of systolic blood pressure(SBP) and diastolic blood pressure (DBP) were also observed. Results   There was an interaction between treatment effects and time effects in serum calcium, phosphorus, calcium-phosphorus product levels in two groups (P < 0.05). With the duration of treatment, there was a trended to decrease and increase respectively in DCa 1.25 group and DCa 1.5 group, and trended to stabilize for therapy 3 to 6 months. There was no interaction between serum PTH and BAP levels and time effects in two groups. There was no statistical difference in simple treatment effects and time effects. The serum PTH level showed a trend of increase gradually in DCa1.5 group with the duration of treatment. In DCa 1.25 group, the serum PTH level trended to stabilize after 3-month therapy and showed a trend of decrease gradually. There was an interaction between treatment effects and time effects in SBP in two groups, but no interaction in DBP. There was a significant difference in SBP before and after treatment in two groups. The level of SBP reduced tardily with time of treatment and reduced significantly after 3 and 6 -month therapy. Conclusion The application of DCa1.25 dialysate in combination with calcitriol decreased serum calcium effectively and kept the lower limit of normal value, which increased tolerability of active vitamin D and calcium carbonate therapy, and decreased SBP in maintenance hemodialysis patients. Related Articles | Metrics

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The Predictive Value of Elevation in Serum Oxidized Low Density Lipoprotein for Premature Coronary Artery Disease SHI Yanli 1,CHE Jingjin 2, LI Lian 2,ZHANG Yanyan 2 2013, (7):  0-646 .  Abstract ( 524 )   PDF (2041KB) ( 2166 )   Objective    To investigate the characteristics of serum oxidized low density lipoprotein(oxLDL) and traditional cardioascular risk factors of premature coronary atherosclerotic heat disease(PCAD)patients. Method    We continuously selected 70 PCAD patients who have accepted coronary artery interventional therapy and 60 patients with normal coronary artery confirmed by angiography to be control group, male patients in two groups were 31-55 years old and female patients were 42-65 years old. Compareted the levels of ox-LDL, high density lipoprotein cholesterol(HDL-C), triglyceride(TG), serum creatinine(SCr), uric acid(UA), C-reactive protein(CRP) and other serum biochemical indicators and the differences of general clinical materials in the two groups. Results     The PCAD group had significantly raised plasma levels at ox-LDL, TG, SCr,UA and percentage of male as compared with the contral group(P＜0.03), but significantly declined plasma level at HDL-C(P＝0.03) and there was no difference at the others indicators. There were positive correlations between UA,SCr and ox-LDL. The regression analysis showed: ox-LDL, HDL-C and SCr had significantly predictive value to PCAD, OR is 1.172、0.144、1.055 respectively. Conclusion    High levels of ox-LDL and SCr, and low levels of HDL-C are the independent predictors of PCAD. Related Articles | Metrics

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Formulation optimization of Salbutamol Sulfate membrane Tablets YANG FENG KUN,YANG DAI LIN 2013, (7):  0-646 .  Abstract ( 578 )   PDF (2149KB) ( 2355 )     Related Articles | Metrics

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Effects of Sevoflurane Postconditioning on Lung Function in Patients Undergoing Cardiac Valve Replacement with Cardiopulmonary Bypass HE Keqiang ,PAN Jianhui ,XIE Zhiqiong ,WANG Ruiting 2013, (7):  0-646 .  Abstract ( 505 )   PDF (2762KB) ( 2139 )   [Abstract]   Objective   To investigate the effects of sevoflurane postconditioning on lung function in patients undergoing heart valve prosthesis implantation with cardiopulmonary bypass (CPB). Methods   Thirty patients, scheduled for heart valve prosthesis implantation, were randomly divided into control group (C, n=15) and sevoflurane postconditioning group (S,n=15). Anesthesia was maintained by intermittent intravenous injection of midazolam, fentanyl and pipecuronium. In group S, 2% sevoflurane was inhaled continuously for 15 min , 2 min before aortic unclamping. Blood samples were taken at 30 min before CPB (T2), 30 min after CPB (T3), 6 h (T4), 12 h (T5) and 24 h (T6) after operation. The values of p（O2）, SaO2, airway peak pressure (Ppeak) and airway plateau (Pplat) pressure during the time of mechanical ventilation were recorded. Oxygen index (OI), alveolar arterial oxygen tension difference (AaDO2), dynamic pulmonary compliance (Cdyn) and static pulmonary compliance (Cstat) were calculated. Results  Compared with group C, the values of SaO2 and Cdynwere significantly higher in group S after 30 min of CPB. The values of OI, p（O2）, Cdyn and Cstat were significantly higher in group S than those of group C after 6h of operation. The value of AaDO2 was significantly lower 12 h and 24 h after operation than that of group C (P < 0.05).Conclusion   CPB led to the lung injury. The sevoflurane postconditioning has a protective effect on lung function in patients undergoing cardiac valve replacement with CPB. Related Articles | Metrics

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Effects of Recombinant Human Fibronectin Fragment Combined with Anti-CD3 Monoclonal Antibody on the Proliferation and Cytotoxicity of Cytokine-induced Killer Cells from Acute Leukemia liqing ,DENG Qi ,LIU Pengjiang ,BAI Xue ,LI Yuming 2013, (7):  0-646 .  Abstract ( 611 )   PDF (2824KB) ( 2312 )   Objective   To investigate the effects of precoated with retroNectin and CD3Ab on the proliferation and cytotoxicity of cytokine-induced killer cells (CIK) from acute leukemia. Methods   Mononuclear cells (MNCs) were isolated from peripheral blood of complete remission AL patients. The MNCs were cultured in vitro precoated with retroNectin(groupⅠ), CD3Ab(groupⅡ), retroNectin combined with CD3Ab(groupⅢ) and cultured by traditional method(control group) to generate CIK. The change of growth rate, phenotypic characterization, secretion of cytokines of CIK, cell cycle, cytotoxicity and apoptosis of CIK were determined. Result     ① The amplification of CIK in groupⅠ（173.19 times）,Ⅱ（153.79 times）and Ⅲ（224.75 times） were higher than that of in control group（110.23 times）, and the amplification of CIK in group Ⅲ was higher than that of in groupⅠ,Ⅱ（P<0.05）. ② The expression of CD25 positive cells in groupⅠ（39.84±8.25）% and Ⅲ（42.16±10.38）% were higher than that of in groupⅡ（11.93±3.47）% and control group（13.76±2.52）%（P<0.01）. ③The percentage of G1 stage cells in groupⅠ（56.92±9.55）% and Ⅲ（54.84±8.83）% were lower than that of in groupⅡ（69.86±7.72）% and control group（69.72±8.44）%, and the percentage of S stage cells were higher（P<0.05）. ④The cytotoxicity in groupⅠ(63.44±12.75)% and Ⅲ（65.02±12.34）% were higher than that of in groupⅡ(50.13±10.53)% and control group(47.96±7.73)%（P<0.05）at the E/T scope 40:1.⑤The percentage of apoptosis cells in groupⅠ（8.9%）and Ⅲ（9.0%） were lower than that of in groupⅡ（35.1%） and control group（25.4%）（P<0.05）. Conclusion   These in vitro studies suggest that we could obtain CIK cells that the proliferation and cytotoxicity were more higher by precoated with retroNectin and CD3Ab. Related Articles | Metrics

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The Effect of Ulinastatin Continuous Infusion combined Rivaroxaban on Coagulation Function in Patients Undergoing Major Orthopedic Surgery YUXi 2013, (7):  0-646 .  Abstract ( 572 )   PDF (2727KB) ( 2201 )   Objective    To explore the effect of ulinastatin continuous infusion combined rivaroxaban on coagulation function in patients undergoing major orthopedic surgery. Methods    Forty-five patients undergoing major orthopedic surgery were randomly divided into three groups：ulinastatin continuous infusion (Uc) group, ulinastatin single injection (Us) group and control (C) group. All the patients received patient-controlled intravenous analgesia (PCIA) after the operation and took rivaroxaban orally 12 hours after the operation. Ulinastatin (5000 U/kg) was given intravenously to both group Uc and Us preoperatively, group C given isometric normal saline, group Uc pumped UTI continuous intravenously at the end of surgery(10000 U/kg) to 48 hours through PCIA pump. Hematocrit(HCT), plasma prothrombin time(PT), activated partial thromboplastin time(APTT), thrombin time(TT) , fibrinogen(FIB) were normally tested before surgery(T1), at the end of the surgery(T2), 12 hours(T3), 24 hours(T4), 48 hours(T5) after the surgery. Results     Compared with T1, TT had a downward tendency after the operation in group C and Us (P <0.05), and FIB ,PT ,APTT were significantly increased from 24 hours after the surgery (P <0.05); In group Uc, the changes of TT showed an steady tendency after the operation (P >0.05), PT and APTT became extended 12 hours earlier than group C (P <0.05), there was no substantial difference in the levels of FIB between the three groups in the postoperative period (P >0.05). Conclusion    During the perioperative period, ulinastatin continuous infusion combined rivaroxaban can improve the hypercoagulable state in patients undergoing major orthopedic surgery,and the clinical efficacy is safe. Related Articles | Metrics

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Effects of Ginsenosides-Rb1 on Connexin 43 in Heart Failure in Rats KONG Hongliang 2013, (7):  0-646 .  Abstract ( 557 )   PDF (3589KB) ( 2265 )   Objective    On the basis of doxorubicin(Dox)-induced heart failure (HF), the present study elucidated whether the effect of Ginsenosides-Rbl (Gs-Rb1), improving cardiac function, was performed by connexin 43 (CX43) and other mechanisms. Methods    Rats with Dox-induced HF were randomly divided into Dox group (n=15) and Gs-Rb1 group (n=17), and the health age-matched rat was as control (n=15); In addition, cardiomyocytes was randomly divided into Dox group, Gs-Rb1 group and control group, in vitro. After the above intervention being performed, echoeardiography or apoptosis ratio (AR) was analyzed, respectively. CX43, p21-activated kinase 1 (PAK2) and protein phosphatase type 2A (PP2A) was assayed by western bolt or Rt-PCR, respectively. Results    1. Gs-Rbl significantly improved LVEF (in vivo), markedly left ventricular weight index (in vivo) and significantly inhibited cell apoptosis (in vitro), all of which were induced by Dox; 2. Both mRNA and protein of CX43, being significantly decreased by Dox, were significantly up-regulated by Gs-Rbl, in vivo and in vitro; 3. There was no significance for PAK1 protein between Dox group and control group, which was markedly increased by Gs-Rbl, in vivo and in vitro; 4. PP2A protein was significantly up-regulated in Gs-Rbl group than in Dox group and in Dox group than in control group, in vivo and in vitro. Conclusion   The effect of Gs-Rb1, improving HF and inhibiting cell apoptosis, was partly performed through adjusting CX43 at least, which may be mediated by PAK1-PP2A. Related Articles | Metrics

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Clinical Analysis of 417 Cases of Mycoplasma Pneumoniae Pneumonia Complicated by Extrapulmonary Complications CHENXiao-Ying ,DONG Hanquan ,GUO Wei ,REN Lixin ,XU Yongsheng ,WAN Liya ,NING Jing 2013, (7):  0-646 .  Abstract ( 567 )   PDF (2903KB) ( 2246 )   [Abstract]  Objective   To investigate the distribution, clinical diagnosis and treatment methods of the extrapulmonary complications in children with mycoplasma pneumoniae (MP).  Methods   The clinical data of 1 100 patients confirmed the diagnosis of mycoplasma pneumonia and with the positive serum MP-IgM test were collected in this study. The distribution and clinical characteristics and MP-DNA detection rates were compared between 417 patients with extrapulmonary complications and 683 cases without complications. The occurrence of various complications in a four-year period was analyzed.Clinical data were compared between fiberoptic bronchoscopy lavage group and non-surgical group.  Results  The MP-DNA detection rate and the length of hospital stay were higher in patients with pulmonary complications than those of patients without complications. The most common types of extrapulmonary complications were liver damage, skin rashes and gastrointestinal reactions , but less severe. Encephalitis, nephritis and myocarditis were rare complications, but severe and occult. The fatal hemophagocytic lymphohistiocytosis (HLH) was also visible in patients. Bronchoscopy lavage was conducive to the recovery of the disease.   Conclusion  MP pneumonia showed high incidence and risks of extrapulmonary complications, which required careful clinical observation and inspection, the dynamic monitoring laboratory markers and comprehensive treatment as well. Related Articles | Metrics

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The Correlative Factors Affecting Elderly Patients’Plasma D-dimer Level. GUOShi-Jie 1,WU Cunjin 1,WANG Lin2,YANG Limin 1,MA JIN PING2 2013, (7):  0-646 .  Abstract ( 684 )   PDF (2724KB) ( 2467 )   Objective  To Research on the risk factors of plasma D-dimer levels in hospitalized elderly patients with internal medicine disease. Methods   Five hundred and seventy eight hospitalized elderly patients were collected from the policlinic for old cadre’s veteran carder clinic of The Second Hospital of Tianjin Medical University. The subjects’ plasma D-dimer was measured by Automatic quantitate latex agglutination rates assay. Subjects were divided into 2 groups: the normal group (less than 0.4 mg / L), and the elevated group (greater than equal to 0.4 mg / L). Record the results of CRP, blood glucose and coagulation conventional test, and the history of diabetes, malignancies and bacterial pneumonia and (or) acute exacerbation of chronic bronchitis of each object. Results    the average value of age, CRP, aPTT, and PT, the rate of diabetes, malignance, and bacterial pneumonia and (or) acute exacerbation of chronic bronchitis in the elevated group were statistically higher than the normal group, but the average value of TC and LDL were statistically lower than the normal group（P<0.05). the plasma D-dimer level had a significant positive correlation with the patient’s age, CRP, diabetes, malignance, and bacterial pneumonia and (or) acute exacerbation of chronic bronchitis (P<0.05). The result of Binary logistic regression analysis showed that age, CRP, diabetes, malignance, and bacterial pneumonia and (or) acute exacerbation of chronic bronchitis (P<0.05). Conclusion   Aging, diabetes, malignance, and acute inflammatory state were the important factors to lead elderly patients’ plasma D-dimer levels to elevate. Related Articles | Metrics

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Single-port transumbilical laparoscopic Surgery in Upper Urinary Tract and Adrenalectomy with report of 130 Cases CHENJING 2013, (7):  0-646 .  Abstract ( 578 )   PDF (2947KB) ( 2211 )   Objective    To summarize the surgical techniques of single-port transumbilica laparoscopic surgery in upper urinary tract and adrenalectomy by using a self made port and evaluate its feasibility and safety. Methods  From March 2010 to November 2011, 130 patients were treated with single-port transumbilical laparoscopic surgery, which included 60 cases of renal cystectomy, 6 cases of pyeloplasty, 2 cases of adrenalectomy, 40 cases of ureteral incision, 10 cases of partial nephrectomy, 12 cases of non-functioning kidney resection. The single-port was made up of two rings and a latex glove. A 2 cm incision was made in the middle of umbilicus, and the single-port was then inserted with three trocars (10,10, 5mm) inside. Tradition laparoscopic equipment and flexible laparoscopic instruments were used to perform the procedures, and the removels were taken out from the port. Results   All laparoscopic operations were successfully completed without conversion to open surgery. The addition of ports was necessary in 6 cases as a result of difficult operations and unclear vision. 60 cases of renal cystectomy: mean operative time was (25.2±4.3) min, mean blood loss was (10.3±2.1)ml, mean postoperative hospital stay was (2.5±0.5)d. 6 cases of renal pelvis：mean operative time was (120.9±18.6)min, mean blood loss was (31.5±9.2)ml, mean postoperative hospital stay was (4.0±1.0)d. 2 cases of adrenal operation: operative time was 50 and 60 min respectively, blood loss was 50 and 51 ml respectively, postoperative hospital stay was 3 and 4 d respectively. 40 cases of ureter incision to take stone: mean operative time was (60.0±9.5)min, mean blood loss was (32.1±9.4)ml, mean postoperative hospital stay was (4.0±1.0)d. 10 cases of partial nephrectomy operation: mean operative time was (134.5±15.2) min, (32.5±7.5) min of renal warm ischemia time, mean blood loss was (52.1±11.4)min, mean postoperative hospital stay was (7.0±1.0)d. 12 cases of renal resection operation: mean operative time was (100.8±9.6)min, mean blood loss was (125.7±25.4)ml, mean postoperative hospital stay was (5.0±1.0)d. Postoperative follow-up for 2-33 months, mean 18.2 months, there were no adverse complications. Conclusions    The laparoscopic single-port transumbilical surgery for upper urinary tract and adrenalectomy lesions is safe and feasible, and the cosmetic results are good, but it should be carefully widespread because of its difficulty in the clinical work. Related Articles | Metrics

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A study on the the distribution and structure of class I integron and ISCR1 in clinical isolates of Acinetobacter baumannii LI Juan 1,HAO Hansheng 2,WANG Yue 3 2013, (7):  0-646 .  Abstract ( 635 )   PDF (2593KB) ( 2218 )   [Abstract]    Objective    To investigate the distribution and the antibiotic resistance genes carried by class Ⅰ integron and insertion sequence common region（ISCR1） among clinical isolates of Acinetobacter baumannii. Methods   Fifty-one clinical isolates of multidrug-resistant Acinetobacter baumannii were collected. Polymerase chain reaction method was used to detect the class Ⅰ integrase gene, variable region of classⅠ integron, ISCR1 and genes related to antibiotics resistance located downstream of ISCR1 in 51 multidrug-resistant Acinetobacter baumannii. Antibiotics resistance genes carried by classⅠ integron and ISCR1 were performed by DNA sequencing. The relationship between classⅠ integron and ISCR1 was detected by PCR-mapping. Results Among 51 multidrug-resistant Acinetobacter baumannii isolates, 45 strains were found containing classⅠ integrase genes, 32 strains were found containing variable regions. Sequencing results showed that the gene cassette arrays were aacA4-catB8-aadA1, aacC1-orfA-orf-B-aadA1 and blaPSE-1-aadA2-cmlA1-aadA1. Twenty-two strains were found containing ISCR1 and 5 strains were found containing resistance genes located downstream of ISCR1. DNA sequencing results showed the resistant gene of qnrA1-ampR. The results of PCR-mapping showed that ISCR1 located directly downstream of 3' conserved segment of classⅠ integron in 20 Acinetobacter baumannii strains. Conclusion ClassⅠ integron and ISCR1 play an important role in mechanisms of drug resistance of Acinetobacter baumannii. ClassⅠ integron and ISCR1 could connect in series in Acinetobacter baumannii. Related Articles | Metrics

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Effects of High Density Lipoprotein on Expression of Alpha-Granulemembrane Protein (CD62P) and Lysosome Intact Membrane Protein (CD63) on Platelet YANG Sheng hua1,DU Dayong 2,LIU Yang 2,WANG Shuangshuang 2,LI Yuntian 2 2013, (7):  0-646 .  Abstract ( 518 )   PDF (2947KB) ( 2251 )   Abstact   Objective   To investigate the effect of High Density Lipoprotein on expression of CD62P and CD63 on thrombin-activated platelet in vitro. Methods   Equivalent volume of washed platelet prepared by hand was preincubated with HDL(1mg/ml) in 37℃ water for 15 minutes ，then stimulated with different concentrations of thrombin(0.5U/ml,1U/ml,10U/ml) for 10 minutes in water of 37℃.Meanwhile another three groups of washed platelet was incubated with thrombin(0.5U/ml,1U/ml,10U/ml) for 10minutes,respectively.The CD62P and CD63 from each sample were analyzed by flow cytometry(FCM). Results   The CD62P positive rate of HDL-preincubated groups were significantly lower than the groups of different concentrations of thrombin(0.5U/ml,1U/ml,10U/ml) in the absence of HDL, 11.55%±1.34% vs 18.14%±1.50%,17.19%±0.17% vs 26.24%±0.77%,19.79%±0.32% vs 80.38%±5.66%;P value <0.001.Meanwhile, The CD63 positive rate of HDL-preincubated groups were also significantly lower the thrombin-treated (0.5U/ml,1U/ml,10U/ml) groups without HDL,namely,8.09%±0.48% vs 2.92%±0.22%(P<0.001),14.15%±1.39% vs 4.20%±0.98%(P<0.001),24.48%±1.71% vs 5.12%±0.09%(P<0.001). Conclusion   HDL inhibits expression of alpha-granulemembrane protein (CD62P) and lysosome intact membrane protein(CD63) on thrombin-stimulated platelet in vitro. Related Articles | Metrics

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The Role of SDF-1 α/CXCR4 Signal Pathway in the Therapeutic Effects of Hypoxic Preconditioning of Endothelial Progenitor Cell Transplantation on Ischemia Heart Disease JIAN Kai tao1,WANG Lian qun1,WANG Qiang 1,YANG Dongyan 1,JIANG Li 1,LIU Jianshi 2013, (7):  0-646 .  Abstract ( 680 )   PDF (2903KB) ( 2500 )   Abstract  Objective   We aimed at SDF-1α /CXCR4 signal pathway on hypoxic preconditioning endothelial progenitor cells(HEPCs), investigating the therapeutic effectiveness of transplantation HEPCs into acute infarcted myocardium Method    Bone marrow endothelial progenitor cells(EPCs) were islolated from syngeneic adult male Wistar rats, normoxic culture on EPCs for 4 days and 1%O2+5%CO2+94%N2 culture for 3 days. Using Boyden Chamber assay to compare migration ability between EPCs and HEPCs towards 100ng/ml bone marrow derived factor -1α(SDF-1α). Using western blot assay to detect expression of CXCR4, which is the solo receptor of SDF-1αon cells surface. Then, 26 syngeneic adult male Wistar rats were randomized into 3 groups: Control(n=8)，EPCs(n=9) and HEPCs(n=9),set up acute myocardium infarction animal model. At infarction, the rats received 5-points peri-infarct intramyocardial injections of PBS 200ul, 2×106 EPCs and 2×106 HEPCs. After 4 weeks, using ECHO to analyze improvement of cardiac function by haemodynamics parameters. Result    Compare with EPCs, the migration ability of HEPCs towards 100ng/ml SDF-1α was increased significant(549±78 cells/HPF vs 118±22 cells/HPF, p=0.009). The result of western blot of CXCR4 revealed that hypoxic preconditioning on EPCs upregulated CXCR4 expression on cell surface. After 4 weeks of transplantation, echocardiography of rats showed that compare HEPCs with EPCs and Control，systolic left ventricular internal diameter(LVIDs), ejection fraction(EF%) improved significantly. Compare HEPCs and EPCs with Control, diastolic left ventricular internal diameter(LVIDd) improved significantly, but there is no difference of LVIDd between HEPCs and EPCs. Conclusion    Regulating SDF-1α/CXCR4 pathway increased treatment effectiveness of cell transplantation and optimized therapeutic strategy on ischemia heart disease. Hypoxic preconditioning of EPCs upregulate CXCR4 expression on cell surface, improve migration ability of EPCs towards SDF-1α through SDF-1α/CXCR4 signal pathway. Transplantation of HEPCs after acute myocardium infarction limited left ventricular remodeling, improved cardiac function entirely. Related Articles | Metrics

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Early changes of left ventricular function after lobectomy in patients with left ventricular diastolic dysfunction li xin,ZHANGPENG 2013, (7):  0-646 .  Abstract ( 749 )   PDF (1350KB) ( 2204 )   Objective  To evaluate left ventricular diastolic dysfunction in patients with combined pulmonary resection of early left ventricular systolic and diastolic function changes. Methods   62 cases with left ventricular diastolic dysfunction in patients before surgery, 3 days after 8 days, respectively, and tissue Doppler ultrasound heart examination, testing reflect diastolic function the peak early diastolic mitral flow velocity (E), mitral peak atrial systolic blood flow velocity (A), E / A , peak early diastolic mitral annulus velocity (Em) peak late diastolic mitral annular velocity (Am), calculation of E / Em; reflect the contractile function of left ventricular ejection fraction (LVEF), mitral annular peak systolic velocity Sm. Continuous monitoring of patient vital signs after surgery. Results   Compared with the previous 3 days after surgery in patients with heart rate, E faster (P <0.05), E / A increased (P <0.05), Em was significantly lower (P <0.01), E / Em significant rise higher (P <0.01), Sm lower (P <0.05), LVEF change was not statistically significant (P> 0.05); and after eight days before surgery compared to patients with heart rate, E, E / A, LVEF and Sm changes are not statistically significant (P> 0.05), Em decreased (P <0.05), E / Em increased (P <0.05). Conclusion   The combined left ventricular diastolic dysfunction in patients early after lung resection, left ventricular systolic and diastolic functions are declining, systolic function earlier than the diastolic function returned to preoperative levels; tissue Doppler examination can be more sensitive detection changes in cardiac function. Related Articles | Metrics

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Principal Component Analysis Hypertension Risk Factors of 618 Baotou Pastoral Area Residents ZHANGSUZHEN ,WANGYING ,YUYANQIN ,LIUCHUNFANG ,LIQIMING 2013, (7):  0-646 .  Abstract ( 648 )   PDF (1571KB) ( 2173 )   Abstract   Objective   To find the most important risk factors of every hypertension patient. Methods   Use logistic regression analysis to filter the risk factors of hypertension; used principal component analysis filter out the most important risk factors of each patient. Results  Hypertension risk factors of Baotou pastoral area are drinking salt tea 、family history of hypertension 、age 、body mass index 、wine age . Body mass index as the main risk factors of hypertension; frequency of drinking salt tea, age, wine age as the main risk factors hypertension accounted for 30.9%; wine age accounted for 19.6 % of its patients with hypertension as the main risk factors; family history of hypertension with its major risk factors accounted for 14.3% of patients with hypertension; one cases of patients with body mass index, age, age is the major risk factors. Conclusion   The pastoral hypertensive patients should take special measures coordinate with drugs to effectively control blood pressure according to their major risk factors Related Articles | Metrics

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Correlation study between serum levels of 1,25(OH)2D3、calcium and FEV1 of patients with Chronic obstructive pulmonary disease QI LI 2013, (7):  0-646 .  Abstract ( 536 )   PDF (1352KB) ( 2133 )   Abstrsct  Objective  To investigate the correlation between serum levels of 1,25dihydroxyvitaminD3(1,25(OH)2D3)、calcium(Ca2+) and FEV1 of patients with chronic obstructive pulmonary disease(COPD) patients. To explore the relationship between the above-mentioned mass and the Severity of COPD. Methods  Subjects were 30 patients with COPD to be parted as acute stage group and stable stage group. We measured the concentration of 1,25(OH)2D3 、serum Ca2+、serum phosphorus(P) in the serum of two stages. We obtained the data of blood gas analysis and pulmonary function tests. Results  The concentration of serum 1,25(OH)2D3、Ca2+ and P were significantly lower in acute stage group than in the stable stage group. The concentration of serum 1,25(OH)2D3 and Ca2+ positively correlated to FEV1（％）.Conclusion  The concentration of serum 1,25(OH)2D3 and Ca2+ were concerned with FEV1（％）,it suggests that above-mentioned mass may potentially prevent the future deterioration of pulmonary function and reduce case fatality rate. Related Articles | Metrics

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Analysis of risk factors of breast cancer after chemotherapy-induced severe myelosuppression WANG Lin ,JIE HAI ,ZHANG ZHENDONG 2013, (7):  0-646 .  Abstract ( 570 )   PDF (2024KB) ( 2751 )   Related Articles | Metrics

循证医学

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A Meta-Analysis of Percutaneous Compression Plate versus Dynamic Hip Screw in the Treatment of Intertrochanteric Hip Fractures in Elderly Patients JIAJun 1,FENG Shiqing 2,LI Weizhe 1,WU Yinghua 1 2013, (7):  0-646 .  Abstract ( 496 )   PDF (3597KB) ( 2268 )   Abstract    Objective   To compare the efficacy of percutaneous compression plate (PCCP) and dynamic hip screw (DHS) for intertrochanteric fractures of the aged. Methods   We searched the Cochrane Library, PubMed, EMbase, CNKI, Wanfang database and other databases. Retrieved the case-control study which used percutaneous compression plate and dynamic hip screw to treat intertrochanteric fractures of the aged, then compared the efficacy of percutaneous compression plate and dynamic hip screw for this kind of fractures by meta-analysis. Results   In 231 citations initially identified, 12 eligible papers, which included a total of 1159 patients followed up, met all the final criteria. We compared the operation duration, blood loss, wound healing, complications, fracture healing and functional outcome et al between PCCP and DHS. The results showed that the PCCP had a better outcome in the operation duration, blood loss, wound healing compared to the DHS, but it didn't show significant difference in the postoperative infection rate and mortality. Conclusions    The current analysis shows that the PCCP ( a quicker operation with minimal blood loss) is better in the older patients, but there should be more larger sample randomized controlled trials to provide decision-making evidence for clinical treatment of intertrochanteric fractures. Related Articles | Metrics

短篇论著

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Early Evaluation of Electrical Diagnostics of Motor Nerve Damage in Carpal Tunnel Syndrome ZHANG ZHECHENG 2,LIU NA 3,TIAN QIAN 3,ZHUJu 2,ZHANG JING 2 2013, (7):  0-646 .  Abstract ( 511 )   PDF (2042KB) ( 2167 )   Abstract   Objective   To early evaluate the function status of motor fibers in patients with carpal tunnel sydrome (CTS) according to the number of motor units estimated (MUNE), the duration and amplitude of motor unit action potential (MUAP) and the amplitude of compound muscle action potential (CMAP). Methods   We evaluated 40 patients, who had been diagnosed with CTS. There were 20 patients in CMAP normal group and 20 patients in CMAP abnormal group. The control group includes 20 healthy people. We used the Keypoint.net(Medoc Ltd)electromyogram device to record the MUNE，the duration and amplitude of motor unit action potential (MUAP) of the abductor pollicis brevis. Results  The MUNE reduced, the MUAP duration prolonged and amplitude highed in the CTS group, There were significantly different from those found in the control group (P<0.05). In CMAP normal group and control group the MUNE was 142.85±21.51 vs 188.23±23.51,(P<0.05), the duration of MUAP was 12.83±1.39ms vs 10.19±1.28ms,(P<0.05)and amplitude 959.60±92.41μV vs 789.45±70.74μV,(P<0.05). The area under the ROC curve was 0.934 for MUNE, 0.919 for MUAP duration and 0.921 for MUAP amplitude. Conclusion   The MUNE ,the duration and amplitude of the MUAP could early evaluate the function status of motor fibers in patients with CTS compared with the CMAP . Related Articles | Metrics

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The Expression and Significant of HBV-M in patients with acute-on chronic liver Failure Ping LI,TANG KECHENG ,CAOWUKUI 2013, (7):  0-646 .  Abstract ( 501 )   PDF (1349KB) ( 2205 )   Abstract   Objective   To explore the Expression and Significant of HBV-M in patients of Acute-on-chronic liver failure（ACLF）. Methods   The data in this study were collected from 117 patients with ACLF and 123 patients with chronic hepatitis B(CHB)、21 patients with acute liver failure(ALF) were analyzed retrospectively. Results   The level of HBV-DNA in acute liver failure group was lowest and the proportion of HBeAg –negative patients in Acute-on-chronic liver failure group was highest in three groups. The level of HBV-DNA was lower and the proportion of HBeAg –negative patients was higher in ACLF group than that of CHB group, the difference were statistically significant in both(P<0．05). There are four patients with ACLF who anti–HBs、anti –HBe and anti –HBc are all positive. Three of them died. Conclusion    Patients with Acute-on-chronic liver failure is characterized by lower level of HBV-DNA and the higher proportion of HBeAg –negative patients than ALF group and CHB group. But it is not related to the severity of illness.The high antibody expression was a particular expression or it was closely related with host's invulnerability, which need further research. Related Articles | Metrics

临床论丛

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Surgical Treatment for Cervical Huge Disc Herination by Anterior and Posterior Approach 2013, (7):  0-646 .  Abstract ( 578 )   PDF (1511KB) ( 2293 )     Related Articles | Metrics

论著

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Effects of Different Ventilation Modes and Parameters on Intragastric Pressure and Digestive Function ZHAO XIAO YUN1,LI YUE CHUANLI GUAN HUA1,ZHANG LI1, JIA Wei 1,JIAO Lina 1,YANG Xuejin 1 2013, (7):  0-646 .  Abstract ( 586 )   PDF (2041KB) ( 2359 )   [Abstract]  Objective   To study the effect on intragastric pressure and digestive function by different mechanical ventilation modes and parameters. Method   The authors selected 40 inpatients from Janurary,2007 to June,2009 who suffered respiratory failure to carry out a prospective study. In different modes and parameters of mechanical ventilation, a multi-channel physiological signal recording device was used to monitor the patients with intra-trachea pressure(ITP), intra-gastric pressure(IGP) synchronously, as well as the emptying time of stomach, PH value and totle bile acid(TBA) concentration in gastric fluid. Result   First, no matter which type of ventilation mode or PEEP parameters was selected, as long as lower ITP, can reduce IGP. The highest IGP was found in CMV group(P<0.01) from the three various of ventilation mode(P<0.01), as well as in PEEP10 group from the three PEEP settings(P<0.01). Second, under mechanical ventilation support, when ITP increased, emptying time of stomach decreased (P<0.01), so as concentration of TBA (P<0.05). However, there was no significant change in PH of gastric fluid(P>0.05). Conclusion   Various mechanical ventilation modes and parameter settings induced different ITP, thereby affecting the IGP and gastric emptying. Maybe we can promote digestive function by choosing the appropriate mechanical ventilation mode and parameters. Related Articles | Metrics

实验研究

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Effect of High-Frequency Stimulation in the Pedunculopontine Nucleus on Neuronal Activity and Neurotransmitters in the Globus Pallidus Internus of Rats Liu Min Jie 2013, (7):  0-646 .  Abstract ( 504 )   PDF (2244KB) ( 2271 )   Objective   To study the effect of high frequency stimulation (HFS) of pedunculopontine nucleus (PPN) on the neuronal activities of globus pallidus internus (Gpi) in Parkinson’s disease (PD) model rats and measure the level of amino acids neurotransmitter. Methods   Seventy male Sprague-Dawley rats were divided into two groups, control group(n=30) and PD model group(n=40). PD model rats were established by the injection of 6-OHDA into SNc with stereotactic technique on the right side of the brain. Electrophysiological recordings were made in anaesthetized rats to investigate the effects of HFS-PPN on the firing rate of the GPi neurons. Brain microdialysis combined with high-performance liquid chromatography was applied to detect glutamate (Glu) andγ-aminobutyric acid (GABA) levels in the GPi. Results   HFS-PPN caused an excitatory reaction of the majority of neurons recorded in the GPi in the PD rats and control group rats, the mean firing rate of GPi excited neurons was significantly increased; The levels of Glu were reduced under HFS-PPN and the levels of GABA were not affected. Conclusion    HFS-PPN heightened the electrical activity of GPi neurons and reduced the level of Glu. This excitatory effects were probably realized by PPN-GPi direct path or other indirect path. Related Articles | Metrics

论著

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The Evaluation and Significance of RANTES in Serum and Peritoneal Fluid in Patients with Endometriosis GAO Guiqin ,LIN Wanjun ,LI Baosen 2013, (7):  0-646 .  Abstract ( 616 )   PDF (3047KB) ( 2174 )   Abstract   Objective    To investigate the role and clinical significance of RANTES in endometriosis. Methods   The level of RANTES was examined by ELISA in serum of 50 patients with endometriosis(EM group), 32 patients with benign ovarian neoplasms (disease control group) and 30 normal control women (normal control group), and the level of RANTES was examined in peritoneal fluid in EM group and disease control group. Results   The level of RANTES in serum (108.73±60.69 ng/L) was more significantly increased in EM group than disease group(31.26±20.33 ng/L) and normal group(29.77±11.58 ng/L)( P<0.05).The level of RANTES in peritoneal fluid (726.31±259.83 ng/L )was more significantly increased than disease group(116.19±81.64 ng/L)( P<0.05). The level of RANTES in serum in EM group was positively correlated with clinical stage (rs=0.501，P<0.01). The level of RANTES in peritoneal fluid in EM group was positively correlated with clinical stage (rs=0.562，P<0.01). The level of RANTES in peritoneal fluid in EM group was positively correlated with dysmenorrhea score(rs=0.527，P<0.01). The level of RANTES in EM group in serum was positively correlated in peritoneal fluid (rs=0.363，P<0.05). The level of RANTES in serum and peritoneal fluid in EM group was positively correlated with inflammatory response degree respectively in endometriosic tissues(rs=0.326，P<0.05; rs=0.391，P<0.01). Conclusion  RANTES plays an important role in endometriosis. The level of RANTES in serum in endometriosis patients is helpful to evaluate the disease degree and may be one of parameters for understanding the endometriosis.RANTES may be as an inflammatory response marker. Related Articles | Metrics

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The Preventive Effects of N-Acetylcysteine on Contrast-Induced Nephropathy in Patients Undergoing Elective Percutaneous Coronary Intervention 2013, (7):  0-646 .  Abstract ( 645 )   PDF (2725KB) ( 2244 )   Abstract   Objective   To investigate the preventive effect of N?acetylcysteine(NAC) on contrast induced nephropathy（CIN） in patients undergoing elective percutaneous coronary intervention（PCI）.Methods   five hundreds and twenty one patients who are about to undergo PCI in Tianjin Chest Hospital are randomly divided into either conventional treatment group（n=261） or NAC treatment group（n=260）. NAC treatment group are received oral NAC (600mg twice daily) for 48h preoperative and 72h postoperative PCI plug hydration, and the conventional treatment group are received only hydration. The levels of blood serum creatinine（Scr）,urea nitrogen（BUN）, creatinine clearance rate （Ccr）, C-reactive protein（CRP）, β2-microglobulin（β2-MG）, tumor necrosis factor-α（TNF-α）, interleukin-6（IL-6）, Superoxidedismutase（SOD）,Glutathione reductase（GSH） were measured at admission and 72h after the procedure. Result   （1）In the NAC treatment group, 16 patients present with CIN，the incidence of CIN was 6.2%;In the conventional treatment group, CIN occur in 10 patients, the incidence of CIN was 3.8%（χ2=1.48，p>0.05）.（2）There was no statistically difference in the levels of Scr,BUN,CRP、β2-MG,TNF-α,IL-6 ,SOD,GSH at admission（P>0.05）. 72h after the procedure, the levels of CRP、SOD、GSH were lower in NAC treatment group than the conventional treatment group, and the levels of CRP,SOD,GSH were higher than preoperative. Conclusion   Oxidative stress and inflammation may play a important role in the pathogenesis of CIN, N?acetylcysteine may not be beneficial in the prevention of CIN. Related Articles | Metrics