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15 June 2013, Volume 41 Issue 6 Previous Issue    Next Issue
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论著
短篇与病例报告
论著
实验研究
短篇论著
临床论丛
综述与讲座
短篇与病例报告


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论著

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Effect of paclitaxel combined with miR-200b on proliferation and invasion of gastric cancer cell line SGC-7901 DU PING1,CONG Ningning 1,SHEN Fajuan 1,KANG chunsheng 2,ZHANG Qing yu1 2013, 41 (6):  513-516 .  Abstract ( 533 )   PDF (422KB) ( 2552 )   Objective    To invesigate the inhibitory effect of paclitaxel combined with miR-200b mimics on proliferation and invasion of human gastric cancer cell line SGC-7901 in vitro. Methods     Human gastric cancer SGC-7901 cells were treated with 50nmol/L paclitaxel alone or combined with transfecting miR-200b mimics. We use the flow cytometry technology, the cell cloning, Transwell assay and wound healing test to observe the change on the the cell cycle, the percentage of cell apoptotic cells, the proliferation ability and the invasive ability. We also discussed the possible mechanism by detecting the level of certain protein using western-blot. Results   The experiments shows that the group treated both with paclitaxel and miR-200b mimics had significantly higher cell apoptotic rate(P<0.001) and lower percentage of cell cloning（P<0.001）, less trans-membrane cell number(P<0.001)，lower migrating rate after 48 h(P<0.001) than the group treated with paclitaxel alone. While there is no significant in the percentage of cells blocked at G2/M phase. As western-blot showed, the level of E2F3 reduced (P<0.001) affter transfecting the miR-200b mimics. Conclusion    MiR-200b may enhance the inhibitory effect of paclitaxel on cell proliferation and invasion of human gastric cancer cell line SGC-7901 by lowering the level of E2F3. Related Articles | Metrics

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Effects of up-regulated miR-200a and miR-141 on gastric adenocarcinoma cell growth in vitro SHEN Fajuan 1,SU Juan 2,ZHANG Qing yu1,KANG Chun sheng3 2013, 41 (6):  517-519 .  Abstract ( 556 )   PDF (886KB) ( 2421 )   Objective    To investigate the effects of up-regulated expression of microRNA(miR)-200a and miR-141 on the invasion and migration capability of SGC7901 human gastric adenocarcinoma cells. Methods   Synthetic miR-200a and miR-141 mimics was transfected into SGC7901 cell line by lipofectamine 2000; control group(without transfection), group B(perfomling transfection with nonsense sequence)and group E(performing transfecfion with both miR-200a and miR-141 mimics)were established．Realtime quantitative PCR was conducted to detect the expressions of miR-200a and miR-141 in these groups after transfection．The migration，invasion capability of cells were evaluated by using transwell chamber model and wound healing assay in vitro, while the expression of E-cadherin, N-cadherin, MMP-9 were determined by Western blot. Results    As compared with those in control group and group B，the expressions of miR-200a , miR-141and E-cadherin increased significantly(P<0.05). The mobility，invasion，migration capability and the expression of MMP-9, N-cadherin proteins of the cells transfected with miRNA mimics were much lower than the other two groups(P<0.05), while the differences between the other two groups were not significant. Conclusion    The up-regulated expression of miR-200a and miR-141 could significantly inhibit the invasion and migration ability of SGC7901 cells. Related Articles | Metrics

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Effects of Programmed Cell Death 4 Gene on Expression of Death Associated Protein 5 and Apoptosis in Colon Cancer Cells YI Bo 1,LI Qiyun 1,RAO Huamin 2,SHAO Jianghua 1 2013, 41 (6):  520-522 .  Abstract ( 693 )   PDF (416KB) ( 2543 )   [Abstract]   Objective   To investigate the effect of exogenous programmed cell death4(PDCD4) gene on the expres- sion of death associated protein5(DAP5) and apoptosis of human colorectal cancer cell LOVO, and involved mechanisms thereof.   Methods   Recombinant eukaryotic plasmid pFLAG/PDCD4was constructed and transfected into human colorectal cancer cell LOVO. Cells stably expressing PDCD4were established by G418selection (500mg/L). The levels of PDCD4protein and mRNA were analyzed by RT-PCR and Western blotting. The apoptotic cells were measured by flow cytometry, and protein expression of DAP5was detected by Western blotting.  Results LOVO-pFLAG/PDCD4cell line was successfully es-tablished by G418selection. Compared to non-transfection and mock-transfection group, the levels of PDCD4mRNA and protein were significantly increased, the cell apoptosis ratio was enhanced and the expression of DAP5protein was increased in transfection group (P<0.01).   Conclusion PDCD4could induce apoptosis of human colorectal cancer LOVO cells, which mechanism might be involved in up-regulating DAP5protein. Related Articles | Metrics

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The Comparative Study of Prognosis in Patients with Oral Tongue Cancer and Base of Tongue Cancer LI SHa sha,ZHOU Xuan ,ZHANG Liuyang ,ZHANG Lun 2013, 41 (6):  523-526 .  Abstract ( 687 )   PDF (368KB) ( 2637 )   [Abstract]  Objective   To compare the prognosis of patients with oral tongue cancer and the base of tongue cancer,and identify the prognostic factors. Methods   The patient records that were first accepted clinical treatments were collected.Patients were divided into oral tongue cancer group and the base of tongue cancer group, according to the tumor location. One hundred oral tongue cancer cases that were matched with fifty base of tongue cancer cases for gender, age, T-stage and tumor differentiation were simultaneously enrolled in the study. The survival rate and disease-specific survival rate were analyzed by Kaplan-Meier analysis. The multivariate analysis was conducted with the Cox regression model in two groups.Results   The three-year and five-year survival rates were 65% and 51% in oral tongue cancer group, and 40% and 28% in base of tongue cancer group. The three-year and five-year disease-specific survival rates were 61% and 46% in oral tongue cancer group, and 38% and 26% in base of tongue cancer group. There were significant differences in three-year and five-year survival rates and disease-specific survival rates between two groups (P＜0.05 or P＜0.01). Multivariate analysis showed that the recurrence (RR=1.431，P=0.019) and regional lymph node metastasis (RR=1.526，P=0.043) were prognostic risk factors.Conclusion   There was a better prognosis in tongue cancer group than that of the base of tongue cancer group. In the present study, the difference in prognosis was closely associated with the recurrence and regional lymph node metastasis in two groups. We concluded that the individual treatment should be used for patients with tongue cancer to improve prognosis. Related Articles | Metrics

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Clinicopathologic Significance of EpCAM and E-cadherin Expression in Urothelial Carcinoma of Bladder SHAN Liping ,ZHANG Mo ,LI Bo ,WU Bin ,SONG Yongsheng 2013, 41 (6):  527-530 .  Abstract ( 649 )   PDF (420KB) ( 2576 )   [Abstract] Objective  To investigate the expressions of the epithelial cell adhesion molecule (EpCAM) and E-cadherin and their clinical significance in urothelial carcinoma of bladder. Methods  The expressions of EpCAM and E-cadherin were analyzed by immunohistochemistry using a monoclonal antibody EpCAM and E-cadherin in 80 cases of normal mucosa and 156 cases of urothelial carcinoma of bladder. The expression levels of EpCAM and E-cadherin were analyzed in different clinical pathological factors. Moreover, EpCAM expression was investigated by comparison with E-cadherin. Results    The positive rate of EpCAM expression in bladder urothelial carcinoma was 53.2%, which was significantly higher than that in para-cancerous mucosa (13.8%，P＜0.01). The positive rate of EpCAM expression was significantly lower in bladder urothelial carcinoma than that in para-cancerous mucosa (35.9% vs 56.3%，P＜0.01). The positive expression of EpCAM was detected in high-grade urothelial carcinoma. The positive expression of E-cadherin was detected in low-grade urothelial carcinoma. The expression levels of EpCAM increased with the increased clinical stages, pathological grades, tumor quantity,distant metastasis and tumor recurrence (all P< 0.05). The expression levels of E-cadherin decreased with the increased clinical stages, pathological grades, tumor quantity and distant metastasis (all P< 0.05). There was a negative correlation between expressions of EpCAM and E-cadherin in bladder urothelial carcinoma (r =-0.607, P < 0.01). Conclusion    The expressions of EpCAM and E-cadherin are associated with tumor malignancies, such as invasion and metastasis. The evaluation of the two markers has a certain value for determining tumor invasion and metastasis. Related Articles | Metrics

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Effects of Different Treatments on the Coagulation Function after Off-Pump Coronary Artery Bypass Grafting QIAN Zhaoyang 1,WANG Zanxin 1,JIANG Yuemei 1,Men Jianlong1,WEI MIN XIN2 2013, 41 (6):  531-534 .  Abstract ( 747 )   PDF (374KB) ( 2497 )   [Abstract]   Objective   To investigate effects of different treatment options on the influence of factor Ⅶ(FⅦ), Von Willebrand factor (vWF), D-dimer and plasminogen activator inhibitor-1 (PAI-1) after off-pump coronary artery bypass grafting (OPCAB). Methods    Sixty patients were divided into two groups: A group and B group, according to the using time of low-molecular-weight heparin (LMWH) and aspirin. The levels of FVII and vWF were examined by ELISA-chromogenic substrate method before operation, 4-d and 14-d after operation. The level of PAI-1 was detected by sandwich technique,and D-dimer was detected by immunogold filtration assay. The level of D-dimer was reviewed in 1 month and 3 months after surgery. All results were analyzed between two groups. Results   The value of FⅦdecreased significantly 4-d after operation in two groups of patients, vWF and PAI-1 increased significantly and returned to preoperative level on the 14-d after operation (P < 0.05). There were no significant differences in three indicators between two groups (P > 0.05). The level of D-dimer increased until 1 month after surgery and went back to preoperative level in 3 months after operation. There was a significantly lower level of D-dimer in B group than that of A group on the 14th day and 1 month after surgery (P < 0.05). Conclusion   Patients have higher levels of coagulation and fibrinolysis function after OPCAB. Postoperative extend the use of LMWH has no impact on FⅦ, vWF and PAI-1 in 14 days after surgery, but which can reduce the level of D-dimer. Related Articles | Metrics

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Prevalence and Risk Factors of Hypertension in Police Officers in Tianjin LI Ying 1,TIAN Feng shi2,YUE Song 3,JIA Junting 3 2013, 41 (6):  535-537 .  Abstract ( 950 )   PDF (310KB) ( 2470 )   [Abstract] Objective   To explore the prevalence of hypertension and its risk factors in police officers in Tianjin.Methods   Based on the clinical data of periodic heath examination in a total of 3189 individuals, a questionnaire survey and physical examination were conducted. Univariate and multivariate logistic regression methods were used to analyze risk factors of hypertension. Results    Of all participants, 1376 individuals (43.1%) were diagnosed with hypertension. The study population was grouped by 21- years old, 30- years old, 40- years old and 50-60 years old. The prevalence of hypertension was significantly difference in different age groups（χ 2=241.864，P < 0.001). The results of multivariate logistic regression analysis indicated that the risk factors of hypertension were associated with age, family history of hypertension (OR=1.588，95%CI=1.320-1.910), diabetes history (OR=1.597，95%CI=1.038-2.457), salty diet (OR=1.285，95%CI=1.034-1.596), total cholesterol (OR=1.171，95%CI=1.056-1.298), and triglyceride (OR=1.323，95%CI=1.208-1.449). Conclusion   There is high prevalence of hypertension in police officers in Tianjin. The risk factors of the hypertension are age, family history of hypertension, diabetes, salty diet, total cholesterol and triglyceride. Related Articles | Metrics

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The Cardiopulmonary Adverse Events of Six-Minute Walk Test in Patients with Chronic Obstructive Pulmonary Disease YU Shuyu 1,ZHANG Dongrui 2,LI YUE CHUAN 2013, 41 (6):  538-540 .  Abstract ( 582 )   PDF (357KB) ( 2576 )   [Abstract]   Objective   To investigate the relationship of cardiopulmonary adverse events during6-minute walk test (6MWT) with different lung function and cardiac complications in patients with chronic obstructive pulmonary disease (COPD).  Methods   Eighty-five COPD patients who finished the test were divided into three groups according to their complications, consisted of COPD group（I，n=16), COPD + left ventricular diastolic dysfunction group （Ⅱ，n=31) and COPD + left ventricular diastolic dysfunction + chronic pulmonary heart disease group（Ⅲ，n=38). The values of heart rate (HR) and pulse oxygen saturation（SpO2）were compared between three groups before and after the test of6MWT,10mins after the test of 6MWT.Results There were27cases（31.76%）finished the test of 6MWT.  Results  showed there were abnormal heart rhythms in 19cases（22.35%) , SpO2<0.90in 14cases（16.47%) and HR >120bpm or SpO2<0.90in 15cases（17.65%). There were significant differences in HR before and after the tests between three groups（P<0.05). There were significant differences in SpO2 before and after the tests between group II and group III（P<0.05).   Conclusion   Severe COPD patients with left ventricular diastolic dysfunction and chronic pulmonary heart disease are more likely to occur cardiopulmonary adverse events during the test of6MWT, and the HR and SpO2are not easy to return to the level before the test. Related Articles | Metrics

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The Influence of High-Dose Rosuvastatin on Circulating Endothelial Progenitor Cells SUN Fuqiang 1,GAO Mingdong 2,MA Yudong 1,LIU Yin 2,SUN Genyi 2 2013, 41 (6):  541-544 .  Abstract ( 697 )   PDF (456KB) ( 2558 )   [Abstract]   Objective  To observe different doses of rosuvastatin on circulating endothelial progenitor cells at 24 hours before coronary angiography (CAG), and to explore the mechanism of therapeutic benefit of high-dose statin before percutaneous coronary intervention (PCI). Methods   Forty-two cases who were to undergo CAG were randomly divided into two groups: 10 mg group (n=20) and 20 mg group (n=22). All subjects took rosuvastatin at a draught. Before treatment and 24 hours after treatment (before CAG), flow cytometry was used to test the number of circulating endothelial progenitor cells(CEPCs). According to the results of CAG, the two groups were respectively divided into two subgroups:coronary artery disease (CHD) group and CAG negative group. Results  After treatment, the numbers of CEPCs in two groups were not significantly changed (10 mg group：49.95±24.57 vs 42.95±21.18, P > 0.05; 20 mg group：57.68±17.62 vs 48.32±22.47, P > 0.05).There was no significant difference in the changed number of CEPCs between 10 mg group and 20 mg group (7.00±18.94 vs 9.36±22.44, P > 0.05). The subgroup analysis showed that the number of CEPCs was significantly increased in CHD group than before treatment with 20 mg rosuvastatin（56.92±12.27 vs 42.08±21.27 , P < 0.05). Conclusion   Taking 20 mg rosuvastatin in patients with CHD at 24 h before PCI increases the number of CEPCs, which may be one of the mechanisms of benefit of high-dose statin therapy before PCI. Related Articles | Metrics

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The Expression and Clinical Significance of COX-2 and 15-PGDH in Esophageal Cancer and Cancer Cell Strain（EC9706） ZHAO Jianqiu 1,LU Xiangdong 1,2,ZHANG Zhi guang3,XIA Xiuli 1,ZHONG Jiangming 1 2013, 41 (6):  545-547 .  Abstract ( 614 )   PDF (549KB) ( 2475 )   Objectives:  To study the expression, clinical significance of COX-2 and 15-PGDH in esophageal squamous cell carcinoma and cancer cell strains. Methods : The expressions of COX-2 and 15-PGDH were determined with immunohistochemical (PV-9000) method in normal esophageal mucosa (n=35 patients), esophageal squamous cell carcinoma (n=35 patients), and esophageal cell strains (EC9706). The expression of mRNA of 15-PGDH was determined with RT-PCR method in normal esophageal mucosa and esophageal squamous cell carcinoma. Results:   The expression of COX-2 was significantly higher in esophageal squamous cell carcinoma than in normal tissue (P < 0.01). The expression of 15-PGDH and 15-PGDHmRNA were significantly lower in esophageal squamous cell carcinoma than in normal tissue (P < 0.05，P < 0.01).The expressions of COX-2 and 15-PGDH were significantly associated with invasive depth of carcinoma tissue and the status of lymph node metastasis (P<0.05). The expressions of COX-2 and 15-PGDH were not significantly associated with sex, age and tumor differentiation. A high expression of COX-2 and a low expression of 15-PGDH were observed in esophageal cell strains. Conclusions:  Compared to normal tissue, a higher expression of COX-2 and a lower expression of 15-PGDH were found in esophageal squamous cell carcinoma and cancer cell strains. COX-2 and 15-PGDH may play an important role in the occurrence and development of esophageal carcinoma. Related Articles | Metrics

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Influential Factors of Cancer-Related Fatigue in Advanced Cancer Patients YANG Juanli ,CHEN Lu ,YANG Shufang ,LIU Xiaoli 2013, 41 (6):  548-551 .  Abstract ( 720 )   PDF (329KB) ( 2745 )   [Abstract]  Objective  To investigate the incidence and influential factors of cancer-related fatigue in advanced cancer patients. Methods   The clinical data of 95 advanced cancer patients were retrospectively surveyed and the questionnaire were used together in the study including Multidimensional Fatigue Symptom Inventory-Short Form,Visual Analogue Scale,Hospital Anxiety and Depression Scale and Social Support Rating Scale. Results    The total of 83 cases (87.37%) experienced fatigue. There was no significant difference in the fatigue between demographic characteristics (P > 0.05). The levels of hemoglobin, white blood cells, serum albumin, Karnofsky performance scale (KPS), VAS pain score, the total social support score, subjective support, support availability, anxiety and depression were significantly related to the fatigue (P < 0.05). Multiple regression analysis indicated that pain，anemia，depression and subjective supports were major risk factors of cancer-related fatigue.Conclusion   There is a high incidence of severe fatigue in advanced cancer patients. The improvement of complications, such as pain, depression, anemia, and increase of subjective support may alleviate the fatigue and improve the quality of life in advanced cancer patients. Related Articles | Metrics

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Role of Streptococcal M Protein Homologous Keratin Polypeptide in the Pathogenesis of Psoriasis GAO Cuiping 1,ZHANG Litao 2,YU Guangxin 2,SONG Mengmeng 2 2013, 41 (6):  552-555 .  Abstract ( 905 )   PDF (401KB) ( 2559 )   [Abstract]   Objective   To study the role of the keratin peptides homologous with M protein in the pathogenesis of psoriasis. Methods   Keratin peptides homologous to M protein were synthetized chemically. The sequences of amino acid residues for these peptides were ALEEAN, LKKDVDC, GLRRVLDE and RLKYENELA, respectively. The levels of interleukin (IL)-2, IL-4 and interferon (IFN)-γ were detected by double-antibody sandwich ELISA assay. The proliferation of peripheral blood mononuclear cells (PBMCs) was detected by MTT method. The keratinocytes (HaCaT) were co-cultured with synthetic peptides. The proliferation of HaCaT was detected by MTT method. Results   The expression levels of IL-2, IL-4 and IFN-γ were significantly higher in psoriatic PBMCs cultured with synthetic ALEEAN and LKKDVDC than those in normal PBMCs. These three cytokines were not detected in the cell culture supernatants of homologous peptides GLRRVLDE and RLKYENELA. The proliferation of HaCaT was promoted in synthetic peptides ALEEAN, LKKDVDC and GLRRVLDE co-treated with keratinocyte growth factor (KGF) (P < 0.05). Conclusion    The keratin peptides homologous with M protein could promote proliferation of PBMCs and HaCat in patients with plague psoriasis, which may play a certain role in the pathogenesis of psoriatic aggravation and maintenance after hemolytic streptococcus infection. Related Articles | Metrics

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Correlations between Prognosis, Metastasis and Expression of Rac1 and NF-κB p65 in Non-Small Cell Lung Cancer YAO Peiyu 1,ZHANGXUN 2,XUMEILIN 3,WANGJING 3,HANHONGLI 2,XUJUNYI 2 2013, 41 (6):  556-560 .  Abstract ( 661 )   PDF (779KB) ( 2543 )   Objective   To investigate expression of Rac1 and NF-κB p65 in Non-Small Cell Lung Cancer(NSCLC) and their clinical significance.Methods   Immunohistochemical staning was applied to detect expression of Rac1 and NF-κB p65 in NSCLC group and control group.Correlations of expression of Rac1 and NF-?B p65 to clinical pathological parameters and prognosis and their correlations were analysed.Result    The expression intensities of Rac1 and NF-κB p65 in NSCLC were distinctly higher than those in control group.A correlation between the expression of Rac1 and NF-κB p65(r=0.656,P<0.001) was also associated with malignancy of NSCLC such as poor differentiation,high TNM stage and lymph node metastasis(P<0.05 or P<0.01). The 3-year survival rates were lower in patients with Rac1 high expression(35.29%) than those with low expression(79.07%) while the 3-year survival rates were lower in patients with NF-κB p65 high expression(40.28%) than those with low expression (74.36%).Further multivariate analysis suggested that both high expression of Rac1 and NF-κB p65 and lymph node metastasis were independent prognostic indicators for NSCLC(P<0.05,P<0.001).Conclusion     The expression of Rac1 in correlates with the expression of NF-κB p65 in NSCLC, which contributes the malignancy-related of NSCLC. Detecting the expression of Rac1 combined with NF-κB p65 in NSCLC tissue may give a clue on prongnosis of patient. Related Articles | Metrics

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Study on Hybrid Single Utility Port Video-assisted Thoracoscopic Surgery Lobectomy for Patients with Early-stage Lung Cancer ZHANGXUN 2,Dong-Bin WANG3,HANHONGLI 2,XUJUNYI 2 2013, 41 (6):  561-564 .  Abstract ( 632 )   PDF (371KB) ( 2668 )   Abstract    Objective   To explore the clinical outcomes of hybrid single utility port video-assisted thoracoscopic surgery (VATS) lobectomy for patients with early-stage lung cancer. Methods    From December 2010 to June 2012, 170 surgery patients with early-stage lung cancer were collected and divided into the experimental group and the control group, the experimental group was undergone hybrid single utility port VATS lobectomy (95cases) and the control group was undergone simplemini-incision lobectomy (75cases). The perioperative indicators and the serum levels of humoral immunity were compared between the two groups. Results     There were statistical differences in incision length (4.68±0.24) cm vs. (10.14±1.54) cm, intraoperative blood lose (173.16±31.56) ml vs. (205.93±39.35) ml, numeric pain intensity scale (5.00±1.06) vs. (6.11±1.23), postoperative inpatient days (9.84±0.92) d vs. (12.07±1.46) d between the two groups (P﹤0.05). There were no statistical differences in operation time (140.40±28.25) min vs. (134.53±31.11) min, lymph node dissection number (11.43±1.74) vs. (11.12±1.55), and incidence of serious postoperative complications (13.7％ vs. 24％) between the two groups. The serum levels of C-reactive protein was significantly lower at 1day, 6 day after operation in the experimental group than those of the control group (P﹤0.05). There were statistical differences in the humoral immunity factors，include IgG, IgA, IgM, at 1day, 6 day after operation between the two groups（P﹤0.05）. Conclusions     Hybrid single utility port VATS Lobectomy is safe and reliable for patients with early-stage lung cancer, which is less injury and better immunity recovery than simplemini-incision lobectomy. Related Articles | Metrics

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Short-Term Clinical Outcomes of Primary Rotating Hinge Knee Arthroplasty for Knees with Severe Deformity XIAO Yu 1,ZHANG Fujiang 2,MA Xinlong 2,REN Kaijing 2 2013, 41 (6):  565-568 .  Abstract ( 626 )   PDF (429KB) ( 2600 )   Objective   To explore the indication and observe short-term clinical outcome of primary rotating hinge knee arthroplasty for knees with severe deformity. Methods   A retrospective study of the clinical outcomes from Oct 2007 to Oct 2010 was done on 60 knees in 51 patients after receiving the TKA with the Endo-Model rotating hinge prostheses. There were 17 males(21 knees),34 females(39 knees),with the mean age of（65.2±8.3）years(ranged 53-78 years).All patients were evaluated pre and postoperatively using X-ray image, the Knee Society Score(KSS) and ROM and the flexion. Results   The average follow-up time was 33 months. The mean operation time was(85.0±14.2)min。No complications of infection, thrombosis and vascular and nerval injury. The ROM and flexion of knees were improved from average 57.4°±15.6°and 78.9°±20.4°to 95.3°±10.2°and 103.6°±12.8°respectively. The KSS clinical score and function score were improved from average 38.6±12.7 and 36.3±15.1 to 87.9±12.5 and 68.8±18.1 respectively。There were 17 patients having anterior knee pain and we could see their patellars shift or tilt in the axial view of X-ray. Conclusion    The short-term clinical outcomes of Endo-Model rotating hinge TKA are ideal, and it is a good therapy choice for knees with severe deformity. However, the high rate of patellofemoral joint complications should be encountered. Related Articles | Metrics

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Effect of Tulobuterol Patches on Infant Bronchiolitis WANG Yanli ,LU Xiaoxia ,WANG Ying ,CHEN Hebin ,CHEN Peng 2013, 41 (6):  569-571 .  Abstract ( 915 )   PDF (321KB) ( 2944 )   【Abstrct】 Objective   To compare the effect of tulobuterol patches and nebulized salbutamol on Infant Bronchiolitis，meanwhile observe the medicine adverse reaction. Methods   Sixty bronchiohtis infants were randomly divided into observation group and control groups. There were 30 cases in each group. The observation group was given tulobuterol patches combined with nebulization Ipratropium Bromide and budesonide, whereas the control group was given inhalation of salbutamol, Ipratropium Bromide and budesonide. Besides them, other therapies (including Oxygen inhalation, sputum suction, symptomatic and support treatment, the use of antibiotics) had no difference. Symptom scores of the coughing and wheezing, the effect scores of daytime lives and nocturnal sleep and the incidence adverse reactions were recorded. Pulmonary function test were detected on the first day and the seventh day. Blood routine, urine routine and medicine’s side effects were observed also.Results   Between two groups, there were no significant difference in the total effect, the level of cough, expectoration, wheezing and the scores of daytime lives（P > 0.05）, but nocturnal sleep was improved significantly（Z=1.974，P < 0.05）. Tidal volume, TI/TE, TPTEF/TE and VPEF/VE had significant difference after treatment in both groups.（P < 0.05）. All of the index were no difference between two groups on the first day. TPTEF/TE and VPEF/VE in observation group were better than those in control group（P < 0.05）. White count and urine routine were not influenced. There was no serious side effect during the treatment. Conclusion   Tulobuteml patches combined with nebulized Ipratropium Bromide and budesonide have a certain effect in treatment of infant bronchiohtis，with faster effect，fewer adverse reactions and good compliance． Related Articles | Metrics

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A Study on Correlation between Cholesteryl Ester Transfer Protein and Coronary Heart Disease LIU Yi 1,LIU Yin 2,GAO Jing 2 2013, 41 (6):  572-575 .  Abstract ( 669 )   PDF (396KB) ( 2645 )   Abstract   Objective    To Investigate the role of Plasma apolipoprotein A1 (ApoA1)/apolipoprotein (ApoB) and other lipid parameters in the occurrence and development of coronary heart disease. Method    In the Year 2010-2011, 550 cases underwent coronary angiography (CAG) in Tianjin Chest Hospital, were divided into coronary heart disease group and normal group according to the result of CAG. Coronary Heart Disease Group includes single-vessel disease group, double vessel disease group, and three vessel disease group. Plasma apolipoprotein A1 (ApoA1), apolipoprotein B (ApoB), total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and apolipoprotein ApoA1/ApoB (ApoA1 / B) were measured and analyzed respectively in all groups. Results    The value of ApoA1/ApoB in coronary artery disease was statistically significant (P<0.01); Multivariate Logistic regression analysis confirmed that the ApoA1 /Apo B was protective factors for coronary atherosclerosis (P＜0.01). Conclusion    The value of ApoA1/ApoB has meaningful relation in diagnosis and treatment of different degree of coronary atherosclerosis in comparison with other relative lipid factors. Related Articles | Metrics

短篇与病例报告

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Neurocysticercosis patients with hydrocephalus shunt concurrent cysticercosis meningitis :case report 2013, 41 (6):  575-575 .  Abstract ( 680 )   PDF (886KB) ( 2442 )     Related Articles | Metrics

论著

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Study of Nasal Ventilatory Function in Patients with Severe Obstructive Sleep Apnea Hypopnea Syndrome XIAO Caixia1 1,LU Honghua 2,RUAN Hongying 2,LIN Peng 3 2013, 41 (6):  577-579 .  Abstract ( 669 )   PDF (437KB) ( 2820 )   Abstract   Objective   TO study the nasal ventilatory function of severe obstructive sleep apnea hypopnea syndrome(OSAHS) patients without nasal obstruction and nasal anatomy anomalies. Methods    102 healthy controls and 77 patients with severe OSAHS without nasal obstruction and nasal anatomy anomalies were included in this study. Acoustic rhinometry was used to measure the minimum cross-sectional area(MCA),distance of the minimal cross-sectional area to the nostril(DCAN) and the nasal volume from 0 to 5cm,2 to 5cm(V5, V2-5). Rhinomanometry was used to measure unilateral nasal inspiratory resistance (IR) and expiratory resistance (ER) at 150 pa,total bilateral inspiratory resistance(TIR) and total bilateral expiratory resistance(TER) were obtained by calculation. Rhinospirometer was used to measure unilateral inspiratory capacity(IC) and expiratory capacity(EC),and the nasal partitioning ratio(NPR) can be calculated. Results     Compared to control group, IR,ER,TIR, TER, IC, EC, NRPi and NRPe were all significantly higher (P<0.05) while V5 and V2~5 were significantly lower(P<0.05) in the OSAHS group. Conclusion     Nasal ventilatory function is abnormal in the severe OSAHS patients without nasal obstruction and nasal anatomy anomalies; pharyngeal obstruction in the OSAHS could cause the change in the nasal ventilatory function. Related Articles | Metrics

实验研究

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Screening of C2C12-GLUT4HA Skeletal Muscle Cell Line QIU Lihong 1,GAO Jing 2,NIU Wenyan 1 2013, 41 (6):  580-582 .  Abstract ( 661 )   PDF (433KB) ( 2602 )   Objective    To select a GLUT4HA-overexpressing C2C12 cell line with contractile activity. Methods    C2C12-GLUT4HA cell line stably overexpressing GLUT4HA was created and 23 of these clones were selected. The differentiation and contraction ability, as well as the expression level of GLUT4HA protein were detected.The clones with good myotubes ,contraction ability and high GLUT4HA protein level were divided into insulin group and its control, electrical pulse stimulation(EPS) group and its control,the amount of GLUT4HA on the cell surface was measured by enzyme-linked immunosorbent assay (ELISA) after stimulation by insulin and EPS. Results   Among the selected 23 clones, clone 17 has higher GLUT4HA protein level, good myotubes and contraction ability. In addition, GLUT4HA in clone 17 myotubes can translocate to cell surface under insulin and EPS stimulation. Conclusion     A contractile C2C12 muscle cell line stably overexpressing GLUT4HA was created. This cell line will be useful to study the mechanism of insulin- and contraction stimuli-regulated GLUT4 traffic. Related Articles | Metrics

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Regulatory action of Ruangan Huajian granule on hepatic expression of Bcl-2 and Bax in C57 mice with experimental hepatic fibrosis YE Lihong 1,WANG Chongkui 2,LIU Lian 1,YANG Li 1,ZHAO Zhaoxia 1,TIAN Yueyang 1,DAI Erhei 1 2013, 41 (6):  583-586 .  Abstract ( 943 )   PDF (815KB) ( 2620 )   [Abstract]    Objective   To study the effect of Ruangan Huajian granule on hepatic expression of apoptosis related of gene expression in C57 mice with experimental hepatic fibrosis. Methods    Forty C57 mice were randomly divided into four groups, i.e. normal control group, model control group, model Ruangan Huajian Granule low-dose group and high-dose group. There were 10 mice in every group. C57 mice with experimental hepatic fibrosis were induced by intraperitoneal injection of 10% CCl4. The expression of Bcl-2和Bax in liver tissue of mice was observed by immune histochemical technique. The score of masculine grade and fibrosis stage (S) in liver tissue were also analyzed. Results    Bcl-2和Bax were Mainly express in coagulative liver cells , Model control group particularly obvious. The expression of Bcl-2和Bax and the fibrosis stage in model groups were significantly higher than those in normal control group (P<0.05), the ratio of Bcl-2/Bax Significantly lower than normal control group . the expression of Bcl-2 in Ruangan Huajian Granule low-dose and high-dose group obvious enhancement than those in normal control group and model control group（P＜0.05）, while the expression of Bax signifecantly reduce（P＜0.05）, the ratio of Bcl-2/Bax Significantly higher than the normal control group and model control group（P＜0.01，P＜0.01）. Conclusion   Ruangan Huajian granule can modulate the expression of Bcl-2 and Bax in liver tissue ,and inhibit the liver cell apoptisis , Therefor it possesses anti-fibrotic effect. Related Articles | Metrics

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The Cytotoxic Dressing Effects and Antimicrobial Activity of Microspheres Containing Levofloxacin WANGXiao-Hui 1,GAO Ping 1,HUANG Shujie 2,GUAN Jing 2,TAO Zunwei 1,LI Yimin 1 2013, 41 (6):  587-589 .  Abstract ( 654 )   PDF (335KB) ( 2584 )   Objective   To study the antibacterial effect of wound dressing with microspheres containing levofloxacin in the live animal experiments and it’s cytotoxicity. Methods    According to ISO 10993-5:2009, study chitosan - cytotoxic effects of wound dressing with microspheres containing levofloxacin; vaseline gauze as a blank control, nano-silver trauma attached to experimental, the dressing as the experimental group, comparing different dressings after covering the wound, the number of colonies of the wound tissue, tumor necrosis factor (TNF-α) levels. Results    The vitro cytotoxic response of the dressing was 0/0. the number of colonies in 0.25,1,4,7 D were 96 ± 9 × 105CFU / g, 435 ± 235 × 105CFU / g, 5 ± 3 × 105CFU / g, 18 to 0; ± 9 × 105CFU / g; TNF-α levels in 0.25,1,4,7 D were 44.37 ± 3.39 Pg / mL, 41.45 ± 3.28 Pg / mL, 36.07 ± 3.63 Pg / mL, 32.91 ± 5.74 Pg / mL. Conclusion     the wound dressing was no significant cytotoxicity. with the growth of administration time, reduce the number of colonies and TNF-α, the wound dressing is a long-lasting antibacterial ability of medical dressings. Related Articles | Metrics

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The Expression and Significance of EMMPRIN-MMPs-VEGF System in Lung Metastasis of Breast Cancer Model LIAi-shan ,GAN Ping 2013, 41 (6):  590-592 .  Abstract ( 697 )   PDF (634KB) ( 2439 )   [Abstract]  Objective  To investigate the expression and significance of EMMPRIN-MMPs-VEGF system in the mouse model of breast cancer metastasis. Methods   The expressions of EMMPRIN， MMP-2， MMP-9， VEGF， TIMP-1 and TIMP-2 were measured by immununohistochemistry (IHC) in lung metastasis of breast cancer model.Results  The expressions of EMMPRIN, MMP-2, MMP-9 and VEGF were all present in model mice. And the positive rates of EMMPRIN,MMP-2, MMP-9 and VEGF were significantly higher in metastasis group than those of non- metastasis group (P < 0.05).Conclusion   The components of EMMPRIN-MMPs-VEGF system cooperate with each other, and jointly promote tumor cell growth, invasion and metastasis in the mouse model of breast cancer metastasis. Related Articles | Metrics

短篇论著

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Relationship between red cell distribution width，direct-bilirubin and paroxysmal atrial fibrillation in hypertensive patients 2013, 41 (6):  593-595 .  Abstract ( 783 )   PDF (371KB) ( 2555 )   Abstract   Objectives   To study the relationship between red cell distribution width（RDW）,direct-bilirubin（D-BIL）and paroxysmal atrial fibrillation(AF) in patients with hypertension. Method     Eighty patients, who were hospitalized in Second Hospital of Tianjin Medical University from October 2011 to April 2012,were enrolled in this study. The patients were divided into two groups: AF group and control group, according to the history of AF. The baseline clinical data were collected including age, sex, combined cardiovascular diseases, laboratory results and findings from echocardiography. The RDW and D-BIL level were compared and logistic regression model was established to identify the potential risk factors for paroxysmal AF. Results    The levels of RDW and D-BIL were significantly higher（P<0.05）in patients with AF than those in control group. Logistic regression analysis showed D-BIL and left atrial diameter（LAD）were the independent risk factors for the development of paroxysmal AF. Conclusion     The higher level of D-BIL may relate to the development of paroxysmal AF in hypertensive patients. Related Articles | Metrics

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The Clinical Value of HR-HPV on the Diagnosing High-grade Cervical Intraepithelial Neoplasia 2013, 41 (6):  595-596 .  Abstract ( 854 )   PDF (252KB) ( 2636 )   Abstract   Objective  To study the clinical value of HR-HPV on the diagnosing high-grade cervical intraepithelial neoplasia(CIN).Methods: We splited 114 patients with different results of cervical pathology into two groups, group A had 43 cases, including chronic cervicitis and CIN I ;group B had 71 cases, including CIN II-III .The diagnostic efficiency of the cervical cytological screening with TCT,HPV virus testing with genomic extraction kit and Cotesting were contrasted. Results   High-grade CIN was only predicted by cervical cytological testing，HR-HPV，cervical cytological testing combined with HR-HPV testing where, the sensitivity were 83.1%,88.7%,91.5%,the specificity were 14.0%,48.8%,58.1%, the positive-predictive value were 61.5%,74.1%,78.3%, the negative-predictive value were 33.3%,72.4%, 80.6% , the diagnostic efficiency were 57.0 %,73.7%, 78.9%, respectively. The diagnostic efficiency of high-grade CIN by HPV testing and Cotesting were better than cervical cytological testing（p<0.05, p<0.05）.Conclusions    The diagnostic efficiency of high-grade CIN by HR-HPV testing and HR-HPV testing combined with cervical cytological testing was better than cervical cytological testing. Related Articles | Metrics

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Effects of advanced glycation end products (AGEs) on the NO in the HUVECs 2013, 41 (6):  597-599 .  Abstract ( 660 )   PDF (305KB) ( 2682 )   [Abstract]   Objective   To investigate effects of advanced glycation end products (AGEs) modified protein on production of nitric oxide (NO) in cultured human umbilical vein endothelial cells (HUVECs). Methods   HUVECs were co-incubated with AGE-HSA, HSA for 24h at different concentrations. Nitric oxide production and activity of nitric oxide synthase in supernatant were measured by NO and NOS assay kits. The activity of SOD and contents of MDA were determined by SOD and MDA assay kits. Results  1 The contents of NO and activity of NOS in AGEs group were significantly lower than those in HSA group and in a concentration-dependent manner (P <0.05). 2 The activity of SOD was significantly lower than that in HSA group and in concentration – dependent manner (P <0.05). The concentration of MDA was significantly higher than that in HSA group and in concentration–dependent manner (P <0.05). 3 The concentrations of NO in supernatant were positively associated with the activity of NOS and SOD, respectively (P <0.01). The concentrations of NO in supernatant were negatively associated with the contents of MDA (P <0.01). The activity of NOS was positively associated with the activity of SOD (P <0.01). The activity of NOS was negatively associated with the contents of MDA (P <0.01). Conclusion   The oxidative damage of AGEs induced by the HUVECs may play an important role in the decrease of NO in the HUVECs. Related Articles | Metrics

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Effects of Sitagliptin on Vascular Endothelial Function in Type 2 Diabetes LIqing ,ZHOUGUOYAN ,CAOWEIJUAN ,HUANGXIA 2013, 41 (6):  599-601 .  Abstract ( 768 )   PDF (343KB) ( 2821 )   [Abstract]   Objective To investigate the effects of Sitagliptin on vascular endothelial function in in Type 2 Diabetes. Methods   45 patients with Type 2 Diabetes Controlled With combination therapy with insulin or other oral hypoglycaemic agents were run-in to the addition of once-daily sitagliptin 100 mg over a 12-week study period. Before and after the treatment o f 16 weeks, The flow-mediated dilation (FMD), fasting plasma glucose(FPG), 2-h postmeal glucose (2hPG), C-peptide, HbAlc and Lipids were measured. Results   The FMD was significantly increased after treatment (p < 0.001). At 12 weeks, the addition of sitagliptin significantly reduced HbA1c(p < 0.05), fasting plasma glucose and 2-h postmeal glucose(p < 0.001). The plasma C-peptide levels significantly increased(p < 0.05), Whereas The change in BMI and lipids were not significantly different(p＞0.05).There was two hypoglycaemic events during the treatment. There was no severe adverse. Conclusion     sitagliptin can improve the endothelial function in Type 2 Diabetes.For patients with Type 2 Diabetes Inadequately Controlled With combination therapy with insulin or other oral hypoglycaemic agents, sitagliptin improved glycaemic control and was generally well tolerated in patients with type 2 diabetes.Without the significant change in body weight. Related Articles | Metrics

临床论丛

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Investigation of serum CEA, CYFRA21-1 and NSE expression for patient with lung cancer 2013, 41 (6):  605-606 .  Abstract ( 686 )   PDF (311KB) ( 2622 )     Related Articles | Metrics

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Value in diagnosis for retroperitoneal fibrosis by ultrasonography 2013, 41 (6):  606-607 .  Abstract ( 651 )   PDF (549KB) ( 2466 )     Related Articles | Metrics

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Clinical Application of Medicine with Retrograding Silicone Tube Intubation in Nasolacrimal Duct to Cure Chronic Dacryocystitis WANGSHUHONG ,LIUXIUMING ,SONGSHUGUANG ,LIXIAOFENG 2013, 41 (6):  608-608 .  Abstract ( 560 )   PDF (215KB) ( 2530 )     Related Articles | Metrics

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Research on the Ambulation Time after Treating lumbar intervertebral disc herniation under went the posterior pedicle screw-rod fixation combined with cage implantation. LISHUYING ,XUYINGHUA ,RENYANLING ,ZHANGYILONG 2013, 41 (6):  609-610 .  Abstract ( 759 )   PDF (313KB) ( 2633 )     Related Articles | Metrics

综述与讲座

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Application of dehydroepiandrosterone among infertility women with diminished ovarian reserve , 2013, 41 (6):  611-614 .  Abstract ( 687 )   PDF (408KB) ( 2753 )     Related Articles | Metrics

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The effect of PI3K/ Akt/ GSK3βin epithelial ovarian cancer proliferation and drug resistance 2013, 41 (6):  614-616 .  Abstract ( 634 )   PDF (307KB) ( 2830 )     Related Articles | Metrics

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The application of superparamagnetic iron oxide (SPIO) nanoparticle in the study of cancer targeted therapy CHENMINGYI ,TIANJING 2013, 41 (6):  617-620 .  Abstract ( 754 )   PDF (401KB) ( 4611 )     Related Articles | Metrics

短篇与病例报告

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With myalgia, weakness as initial manifestation of type 2 diabetes complicated with microscopic polyangiitis: report of 1 cases PEIYu-mei ,GAOKEHUAI ,WANGXUEJING ,ZHANGGUANGHUI 2013, 41 (6):  623-624 .  Abstract ( 699 )   PDF (293KB) ( 2489 )   无 Related Articles | Metrics