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Tianjin Medical Journal

Table of Content

    15 August 2011, Volume 39 Issue 8 Previous Issue    Next Issue
    论著
    实验研究
    短篇论著
    临床论丛
    短篇与病例报告
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    论著
    The Relationship between the Expression of HIF-1α and STAT3 Molecules and Lymphatic Metastasis of Colorectal Carcinoma.
    2011, 39 (8):  676-678 . 
    Abstract ( 1390 )   PDF (3030KB) ( 2016 )  
    ABSTRACT Objective: Study the mechanism of action of hypoxia inducible factor-1α(HIF-1α), signal transducers and activators of transcription 3(STAT3) in lymphatic metastasis of colorectal carcinoma. Methods: The levels of HIF-1α, STAT3 and VEGF-Ｃ mRNA transcription and protein expression of adenocarcinoma and surgical margin tissues gotten from 60 cases with CRC were determined by real-time quantitative PCR using fluorescence and immunohistochemistry, as well as lymph vessel density was counted using anti-podoplanin monoclonal antibody. And correlations of above indexes and lymphatic metastasis of colorectal carcinoma were analyzed. Results: The levels of HIF-1α, STAT3 and VEGF-Ｃ of tumor tissues were significant higher than that of surgical margin in either mRNA transcription or protein expression(P＜0.05). The levels of HIF-1α, STAT3 and VEGF-Ｃ of tumor tissues were more significant relative not only in either mRNA transcription or protein expression but also in lymphatic metastasis group(P＜0.05). Conclusion: The expression of lymphatic vessels system in colorectal carcinoma is higher than that in surgical marginal tissue. HIF-1α,STAT3 and VEGF-C are the key regulators in lymphatic vessel formation of colorectal carcinoma,and are closely related to lymphatic metastasis.
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    Correlation between the expression of ERα and VEGF in different histopathological variants in patients of Papillar thyroid carcinoma
    2011, 39 (8):  679-681 . 
    Abstract ( 1081 )   PDF (2421KB) ( 2139 )  
    Objective：To investigate the correlation between the expression of estrogen receptor alpha(ERα) and vascular epithelial growth factor (VEGF) in different histopathological variants in patients of papillar thyroid carcinoma(PTC). Methods： Compare with and study the expression of ERα and VEGF which were examined by SP immunohistochemistry in 59 cases of PTC , 56 cases of thyroid adenoma, 30 cases of normal thyroid tissues. Results：The positive expression rate of ERα in cases with PTC, thyroid adenoma and normal thyroid tissue were 83.05%,58.93% and 30% respectively. There was significant difference between　　 PTC and adenoma or normal thyroid tissue(p<0.01).The positive expression rate of VEGF in cases with PTC, thyroid adenoma and normal thyroid tissue were 64.41%,39.29% and negative respectively. There was significant difference between　　 PTC and adenoma or normal thyroid tissue(p<0.01). The ERα and VEGF expression of PTC were related with the histological variant as well as lymph node metastasis（P＜0.05）.The ERα expression was positively correlated with the VEGF expression in PTC(r = 0.514，P = 0.033). Conclusion：The ERα may stimulate proliferation and promote differentiation of the thyroid epithelial cells. The increase of ERα expression may play a role in promote the angiogenesis and development of PTC.
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    Study on the Effect of Optical Imaging Blur on Micro-Saccadic Eye-Movements
    2011, 39 (8):  682-684 . 
    Abstract ( 1420 )   PDF (2391KB) ( 2241 )  
    Objective：To compare the differences of behavioural characteristics of human microsaccadic eye-movements between under normal refractive imaging state and optical imaging blur and to explore the the effect of abnormal visual experience on microsaccadic eye-movements. Methods：From January to March in 2011, 13 cases of subjects with corrected normal or normal vision were recruited from the outpatient clinic at Tianjin Eye Hospital.They were tested respectively under normal optical imaging state (group Nd for dorminant eyes and group Nn for non-dorminant eyes) and optical imaing blur state with -3.00D glass (group Bd for dorminant eyes and group Nn for non-dorminant eyes). A high-speed eye-movement recording system was used to monocularly record the fixational eye-movements of subjects'both eyes. A Matlab routine was used to detect and analyze the microsaccadic components of eye-movement waveforms. The microsaccadic amplitudes, peak velocities, occurrence rates, inter-microsaccadic intervals of all groups were analyzed and compared using Origin8.0 and Matlab2008 statistics toolbox. Results：There is no significant difference among mean microsaccadic amplitudes of all groups. The mean peak velocities of groups Bd and Bn was slower than groups Nd and Nn. The mean occurrence rates of groups Bd and Bn was less than groups Nd and Nn. The mean inter-microsaccadic intervals of groups Bd and Bn was longer than gorups Nd and Nn. There is no difference of peak velocity, occurrence rate and inter-microsaccadic interval under two imaging states betwwen dorminant eyes and non-dormiant eyes. Conclusion：High-speed eye-movement recording could provide useful assitance in evaluation of the visual quality and for exploring the relationship between visual perception and eye-movement behaviours.
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    Virus etiology in children with acute lower respiratory tract infection in Tianjin
    2011, 39 (8):  685-687 . 
    Abstract ( 1501 )   PDF (2287KB) ( 2330 )  
    acute lower respiratory tract infections (ALRTIs). Methods 202 NPA samples were detected by multiplex PCR for ADV, PIV1, 2, 3, Flu A, B, RhinoV A/B, RSV A, B, HMPV, Cov OC43/HKU1, Cov 229E/NL63. Human Bocavirus (HBoV) and WU polyomovirus (WUVyP) were detected by PCR. Results Among 202 NPA samples, 151 (74.8%) were pathogenic respiratory viruses positive. Of 151 virus positive patients, 54(26.7%) were 2 viruses co-infection. The most frequently detected virus was RSV(42.0%), followed by PIV and RhinoV A/B. There was a significant prevalent peak of RSV from December to March in next year. And PIV3 was the predominant pathogen from March to May. 2 serotypes of Cov co-existed in Tianjin. The most commonly co-infected virus was HBoV(61.5%). No significant age distribution of respiratory viruses was found. The prevalence of WUPyV in ALRTIs children was 19.8%. Conclusion RSV was the predominant pathogen in children with ALRTIs, We should focus on the high prevalence of WUPyV in ALRTIs.
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    An clinical study on therapeutical effects of Rhodiola Rosae on cirrhotic cardiomyopathy.
    WANG Xiao Hong
    2011, 39 (8):  688-691 . 
    Abstract ( 1573 )   PDF (2938KB) ( 2147 )  
    【Abstract】　Objective　To explore the effects of Rhodiola Rosae in treatment of patients with cirrhotic cardiomyopathy (CCM). Methods　A total of 40 patients with CCM was randomly divided into group A(n=20) and B(n=20)，treated with routine method (group A) and Rhodiola Rosae capsule added routine method (group B)，respectively. Another 20 normal individuals were used as normal controls. The levels of serum NO were measured by the method of nitric acid reductase. The levels of plasma NT-proBNP were measured by the method of electrogenerated chemiluminescence. LVEF and the ratio of E/A were measured by color Doppler. Results LVEF and the ratio of E/A were significantly lower while the levels of serum NO and plasma NT-proBNP were significantly higher in group A and group B on admission as compared with group C(P<0.05). The levels of serum NO were significantly lower in group B on the 10d after treatment as compared with group B on admission(P<0.05). LVEF and the ratio of E/A were significantly higher while the levels of serum NO and plasma NT-proBNP were significantly lower in group B on the 30d after treatment as compared with group B on admission and group A on the 30d after treatment，and in group B on the 90d after treatment as compared with group B on admission and group A on the 90d after treatment，and also in group B on the 90d after treatmentas compared with group B on the 30d after treatment (P<0.05). Conclusions It is clear that Rhodiola Rosae can play a therapeutic role in CCM.
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    In vitro Susceptibility Assay of 51 Stenotrophomonas Maltophilia and with biofilms to antibiotics
    2011, 39 (8):  692-694 . 
    Abstract ( 1288 )   PDF (4060KB) ( 2081 )  
    Abstract Objective: To investigate the current situations and resistance of Stenotrophomonas Maltophiliaclinical strains collected from Tianjin Area, we investigated the in vitro effects of antibiotics on the SMA biofilms. Methods: The distribution and drug resistant situations of clinical strains of SML isolated were analyzed. Subseqently, The model of SMA biofilms in vitro was developed, challenged by antibiotics and calculated biofilm inhibitory concentrations, and to observe the change of biofilms by scanning electron microscopy. Rusults: The resistance rates of SMA were as following: 13.73％；25.49％；37.25％；45.10％;88.25％；60.78％；49.02％；43.14％；45.10％；94.12％；90.20％；82.35％; Of 42 strains which could be formed bacterial biofilms; The drug experiments and scanning electron microscopeand biofilms formation that, after each antibiotic resistance and resistance strains were increased substantially. Conclusion: SMA may be one of the most important pathogenic bacteria in Tianjin Area, with features of high drug resistance rate and multidrug resistance. The susceptibility testings demonstrate, levofloxacin and cefoperazone/sulbactam have high level antimicrobial activity. Comparing to planktonic bacterial growth, SMA growing as biofilms proves to be highly resistant to most antimicrobials.
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    Analysis of Metabolites in Plasma of Male Hyperuricemia Patients by Metabonomics Methods
    2011, 39 (8):  695-697 . 
    Abstract ( 1571 )   PDF (2545KB) ( 2651 )  
    Objective：To analyse changes of metabolites in plasma of Male Hyperuricemia (HUA)patients by using metabonomics method. Method： The differences of metabolites between HUA group and health group were observed by NMR-based-metabonomics method,and the data was analyzed by principal components analysis (PCA) and orthogonal signal correction(OSC)-partial least squares (PLS) analysis. Results：Compared with health person ,HUA patients had a higher concentration of saturated fatty acid (SFA), N-Acetyl-Glucoprotein (Nac), VLDL,Lactate, threonine, ethanol and the concentration of lecithin, Glutamine,glucose is decreased. Conclusion： The differences between HUA group and health group can be observed by NMR-based-metabonomics method, which may contribute to the analysis of pathological mechanism and development of HUA disease,and it can also be used to analyze the relationship between HUA and Metabolic Syndrome(MS).
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    Retrospective Analysis of characteristics and prognosis of hopitalized Chronic Heart Failure patients with persistent hyponatremia
    jiao-hong HUANG 2, 2, 2
    2011, 39 (8):  698-700 . 
    Abstract ( 1441 )   PDF (2182KB) ( 2046 )  
    Retrospective Analysis of characteristics and prognosis of hopitalized Chronic Heart Failure patients with persistent hyponatremia HUANG Jiao-hong, WANG Lin△, MA Jin-ping, YANG Yan-hua,AN Fang, LI Guang-ping Department of Gerontology, the Second Hospital Affiliated to Tianjin Medical University, Tianjin 300211, China Abstract Objective:To analyze the clinical characteristics of hospitalized Chronic Heart Failure patients with persistent hyponatremia， research the influences of persistent hyponatremia to prognosis. Methods:Collecting cases with chronic heart failure and hyponatremia at admission during recent 30 years, categorizing appropriate cases into persistent hyponatremia group and normalized sodium group on the basis of serum sodium before leaving hospital. Comparing the general characteristics, drug therapy, days of hospitalization and in-hospital mortality between two groups. Results:452 cases were enrolled, among which there were 201(44.5%)cases being persistent hyponatremia,and 251(55.5%) being normalized serum sodium. Patients with persistent hyponatremia were characterized by lower admission sodium, lower systolic blood pressure, lower left ventricular ejection fraction and lower osmosis pressure(128.6mmol/L vs. 131.9 mmol/L,120mmgH vs. 130 mmgH,39.0% vs. 46.7%,285 Osm/kgH2O vs. 291 Osm/kgH2O,respectively.all P＜0.05).Among persistent hyponatremic patients, there were less patients receiving Angiotensin-converting enzyme inhibitors(ACEI) or Angiotensin receptor blocker(ARB),but more receiving diuretics. It was the same of hospital stays between two groups, whereas higher in-hospital mortality in persistent hyponatremic patients（χ2=22.383，P＜0.001）. After adjusting for differences with multivariable analysis, the risk of in-hospital mortality was increased by 2.534 folds in persistent hyponatremic patients compared with normalized patients（χ2=10.515，P=0.001）. Conclusion:Persistent hyponatremia in hospitalized patients with chronic heart failure is characterized by more severe symptoms, poorer responses to therapy and higher in-hospital mortality. Persistent hyponatremia was an independent risk factor of in-hospital mortality.
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    Study on Relationship between Plasma Lipoprotein-associated Phospholipase A2 and Coronary Atherosclerosis
    2011, 39 (8):  704-707 . 
    Abstract ( 1481 )   PDF (2903KB) ( 2273 )  
    Objective:To investigate wether plasma lipoprotein-associated phospholipase A2(Lp-PLA2) activity correlates with severity and stability of coronary atherosclerosis in coronary heart disease(CHD).Method:Coronary angiography (CAG) was performed in 299 patients who were suspected as having CHD.According to the result of CAG,the patients were divided into CHD group(n=232) and control group(n=67).The CHD group was further divided into subgroups according to the clinical types and Gensini scores of every patient was calculated. Lp-PLA2 activity and the levels of plasma total cholesterol(TC)，triglyceride(TG)，low density lipoprotein- cholesterol (LDL-C)，high density lipoprotein- cholesterol (HDL-C)，lipoprotien-a[Lp（a）]，fibrinogen(FIB) and C- reactive protein (CRP) were measured in all groups and the results were compared and analysed .Resultes:Plasma Lp-PLA2 activity in CHD group was higher than that in control group (P<0.01),and that was higher in the group of acute myocardial infarction(AMI) and the group of unstable angina pectoris(UAP)) than that in stable angina pectoris(SAP)(P<0.05).The comparison between AMI group and UAP group showed no statiscal significance.Plasma Lp-PLA2 level was positively correlated with LDL-C,CRP,and Gensini score .Level of Lp-PLA2 correlated independently to CHD. Lp-PLA2 ,hypertension, LDL-C and Lp（a）were independent risk factors of CHD,while HDL-C was a protective factor. Gensini score was effected on age, Lp-PLA2 , HDL-C and LDL-C .Conclusion: Lp-PLA2 is one of independent risk factors of CHD .The plasma level of Lp-PLA2 is related to the degree of coronary artery stenosis and the stability of coronary atherosclerosis.
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    The Study of Relationship beteen Plasma Chemerin in Type 2 Diabetes Mellitus with Nonalcoholic Fatty Liver Disease and Inflammation
    2011, 39 (8):  708-710 . 
    Abstract ( 1465 )   PDF (2142KB) ( 2209 )  
    Abstract Objective:To reveal the relationship between plasma chemerin in type 2 diabetes mellitus (T2DM) with nonalcoholic fatty liver disease (NAFLD) and inflammation. Methods: To select type 2 diabetes mellitus (T2DM) with nonalcoholic fatty liver disease (NAFLD) patients 120 examples and healthy volunteers 60 examples as studied objects, according to the median of plasma TNF-α,we groupe type 2 diabetes mellitus (T2DM) with nonalcoholic fatty liver disease (NAFLD) patients into high-grade group(A group) and low-grade group (B group), healthy volunteers as C group.Detect every group blood glucose, blood lipids, HbA1c, FINS, visceral adipose tissue,hs-CRP, TNF-α,IL-6, chemerin,count HOMA-IR and analyze the relationship of plasma chemerin and inflammation. Results: The level of plasma chemerin in group A and group B were significantly increased compared with controls( P<0.01), The level of plasma chemerin in group A were significantly increased compared with group B( P<0.01).Plasma chemerin level in T2DM with NAFLD was positively correlated with waist circumference、LDL-C、visceral adipose tissue、TNF-a、IL-6. Multiple stepwise regression analysis showed that visceral adipose tissue、TNF-a、IL-6 were independent related fators in influencing the level of plasma chemerin. Conclusion:Chemerin in T2DM with NAFLD is associated with inflammation,which may contribute to the development of T2DM with NAFLD.
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    Clinical Significance of sTREM (soluble triggering receptor expressed on myeloid cells)-l in Patiens with Sepsis
    2011, 39 (8):  717-719 . 
    Abstract ( 1399 )   PDF (2204KB) ( 2138 )  
    Objective: To investigate the clinical significance of sTREM (soluble triggering receptor expressed on myeloid cells)-1 in patients with sepsis. Methods: Using prospective, randomly control study design, 32 patients suffered from sepsis between May 2009 and July 2010 were collected．They were divided into survival group and non-survival group according to 28-day prognosis. 25 health volunteers were controls. Clinical date were determined and APACHEⅡscore were recorded in 24 hours. The differences between them were compared. The level of sTREM-1 were measured by enzyme linked immunoabsorbant assay(ELISA). Results: The level of sTREM-1 was significantly higher in sepsis patients than that of controls[250.9(195.8,354.3)ng/L vs 25.7(21.5,53.3)ng/L, P<0.01]. It was significantly higher in non-survivors(n=11) than that of survivors(n=21) [360.5(262.2,434.5)ng/L vs 204.1(175.0,269.6) ng/L，P<0.01]. APACHEⅡscore was significantly higher in non-survivors(n=11) than that of survivors (t=2.657, P<0.05). The levels of sTREM-1 showed obviously positive correlation with APACHEⅡscore(r=0.426, P=0.032).There were no different between non-survivors and survivors in C-reaction protein(CRP), white blood cell(WBC) and neutrophil count percentage(N), but they were higher in sepsis group than that of in controrls. Conclusion: Plasma level of sTREM-1 is a useful index in monitoring during sepsis.The increase of sTREM-1 was associated with pour prognosis .
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    Research on the effects of low molecular weight heparin combined with dextran-40 on oligohydramnios
    2011, 39 (8):  720-722 . 
    Abstract ( 983 )   PDF (2188KB) ( 2068 )  
    Abstract Objective： To investigate the effects and safety of low molecular weight heparin combined with dextran-40 on pregnant women with oligohydramnios. Methods： A total of 73 pregnant women who were diagnosed as oligohydramnios were randomized into combined therapy group(n=21), low molecular weight heparin (LMWP) group(n=21) and control group(n=32). The combined therapy group was treated with 0.4 mL of LMWP and 500 mL of 5% dextran-40. LMPW group was treated with 0.4 mL of LMPW only. The control group was given 500 mL of 5% glucose infused with 40 mg of Adenosine triphosphate，0.4 g of carnine，100 u of coenzyme A and 250 mL of compound amino acids. Amniotic fluid index(AFI) and coagulogram markers were measured after a course. Results：The improvement rate were significant higher in combined therapy group(90.5%) and LMWP group(80.0%) than in control group(46.9%)（P＜0.05）, but there was no difference between these 2 groups（P＞0.05）. The average increasing volume of AFI was significant different between the 2 groups（P＜0.05）. The combined therapy group(4.41±0.63) was better than LMWP group(3.95±0.57). There was no difference in blood coagulation indication between before and after treatment in each group（P＞0.05）. Conclusion： LMWP combined with dextran-40 could improve volume of amniotic fluid effectively in patients suffered with oligohydramnios, and it was safe to both mothers and fetuses.
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    The research of soft tissue profile in Chineses beauty
    1, 1
    2011, 39 (8):  723-726 . 
    Abstract ( 1387 )   PDF (3762KB) ( 2384 )  
    Abstract 0bjective: To investigate the preferences of Chinese population for facial profile esthetics. Methods：Lateral pictures of famous stars were collected from chinese films, teleplay poster .They were standardized by computer then divided into two groups based on sex.19 mea- surements and 14 Identification Points were made by using Vceph 6.0.Result: The angles of OP -B’LLA，Sn-Stoma have statistical significant between two groups(P<0．05).Conclusions: The Chinese beauty showing upright facial profile and clearly outline soft chin and nose;The aesthetic features is different between male and female.The lower lip slanted at a greater angle in male than in female.
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    实验研究
    Static magnetic field reduced the toxic effect of metal ions on osteoblasts
    2011, 39 (8):  727-729 . 
    Abstract ( 1345 )   PDF (4060KB) ( 1961 )  
    [Objective] Investigate the toxic effect of metal ion C02+,Cr3+ on osteoblast under the static magnetic field,and search a method for preventation and treatment of aseptic loosening.[Methods] CoCl2 powder and CrCl3 powder were dissolved in the asepsis injecting water. Mouse calvarial osteoblasts(MC3T3-E1) were cultured with C02+,Cr3+ ions in different magnetic field of 10Gs，100Gs，1000Gs for 12，24 and 48 h．Observe morphological features of cell growth under inverted microscope . Scanning electron microscopy detect ultrastructure of osteoblasts. Cell apoptosis rate was assured by flow cytometry. ELISA method was appied to detect ALP content in serum supernatant.[Results] Under the static magnetic field,osteoblast showed more mature morphological features; the group 10Gs，100Gs，1000Gs has obviously lower apoptosis rate compared with the expriment group; the A value tested by ALP ELISA method in expriment group up-regulated obviously. [Conclusion] The static magnetic field could reduced the toxic effect of metal ion C02+,Cr3+ on osteoblast, offered the experimental evidence for preventation and treatment of aseptic loosening with magnetic field therapy.
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    Protective Effects of Puerarin Combined with Taurine on Pncreas of Rats with Type 2 Diabetes
    2011, 39 (8):  730-732 . 
    Abstract ( 1396 )   PDF (2587KB) ( 2076 )  
    　Ojective　To investigate the effects of puerarin combined with taurine on pancreas of rats with type 2 diabetes. Methods　40 rats were divided into diabetes mellitus group (DM), Taurine treatment group(Tau), Puerarin treatment group(Pue) and combined with taurine treatment group(Pue+Tau) at random. After having been treated with puerarin or/and taurine for 8 weeks, Glycosylated Hemoglobin (HbA1c) and the serum contents of glucose, insulin, malondialdehyde (MDA), superoxide dismutase ( SOD) and reactive oxygen species(ROS) were measured; the activity of SOD, MDA and ROS in pancreatic mitochondria were detected respectively. Immunohistochemistry technique was used to detect the expression of PDX-1 in pancreas in different group rats respectively. Results Compared with DM group, taurine or puerarin can reduce the content of glucose, MDA, ROS and HbA1c and raise the activity of SOD and the insulin content. The expression of PDX-1 protein was significantly up-regulated in pancreatic islets in Pue+Tau group compared with that of DM group. Conclusion These results suggest that puerarin combined with taurine could prevent the pancreas from oxidative stress in diabetic rats and induced the up-regulation of PDX-1 expression.
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    The Meeh constant which should be used to estimate the body surface area of a C57BL/6 mouse in a thermal injury researching.
    2011, 39 (8):  733-735 . 
    Abstract ( 1488 )   PDF (356KB) ( 2279 )  
    [Abstract] Objective To investigate the Meeh constant(k) which could be used in the Meeh Formula to estimate the body surface area(BSA) of the C57BL/6 mouse when making its thermal injury model. Methods 24 normal C57BL/6 mice of 6-12 weeks age were randomly collected and intook normal diet until the experiment day. On the 3rd day before the experiment day, each mouse was weighted in every 4 hours. The next day, the 24 mice were randomly divided into 3 groups which congtained 4 male and 4 female mice. On the experiment day, the 1st group was anaesthetized, weighed and skinned at 8AM, while the 2nd group at 4PM and the 3rd one at 12PM. The BSA and k value of each mouse was recorded. Results The average weight of the 24 C57BL/6 mice reach its botton at 4PM and its top at 12PM. The calculated k value was 9.617±0.104 at 8AM, 9.802±0.110 at 4PM, and 9.570±0.244 at 12PM. There was statistical difference not only between the k value at 8AM and 4PM, but also between 4PM and 12PM. The k value which was often quoted as 9.1, 9.5, 10 or 11.4 when the literature dealing with the Meeh Formula of mice was reviewed. There was statistical difference not only between the often-quoted k value and the calculated k value at 8AM, but also between the often-quoted k value and the calculated k value at 4PM. Conclusions In the procedure of making the thermal injury model of C57BL/6 mice， it was reliable to use 9.617 as the k value to estimate the BSA at 8AM, but use 9.802 at 4PM.
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    Variation of RB phosphorylation in lung fibroblasts of neonatal rats treated with hyperoxia
    2011, 39 (8):  736-738 . 
    Abstract ( 1255 )   PDF (2311KB) ( 2039 )  
    Objective To explore the variation of RB (retinoblastoma, RB ) phosphorylation in lung fibroblasts of neonatal rats treated with hyperoxia.. Methods In 12 hours after birth, full-term newborn rats were continuously exposed to oxygen (0.85) or room air. Pups were randomly selected to be sacrificed on postnatal 3d, 7d, 14d and 21d, lungs were immediately resected under sterile condition and primary cell culture were done. the protein expressions of RB and phosphorylated RB(p-RB, ser-795) were detected by immunohistochemistry. Results Exposure neonatal rats in hyperoxia for 3 days did not change the expression of p-RB in lung fibroblast.(p>0.05). However, the expression of p-RB in hyperoxia groups was significant upregulated on 7day, 14day and 21day compared with that in air-exposed groups(p<0.05 on 7 day, p<0.01 on 14day and 21day). No notable difference was observed in the expression of RB between hyperoxia groups and air-exposed groups. Conclusion Exposure of neonatal rats in hyperoxia can down-regulate expression of P16 gene, change the phosphorylation of RB protein, ultimately lead the pulmonary interstitial fibrosis.
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    Role of Mito-KATP in Delayed Preconditioning with Sevoflurane Reducing Oxidative Injury Induced by Myocardial Ischemia-Reperfusion in Rats
    2011, 39 (8):  739-742 . 
    Abstract ( 924 )   PDF (463KB) ( 2018 )  
    Objective：To investigate the role of mitochondrial ATP-sensitive potassium channel (mito-KATP) in delayed preconditioning with sevoflurane reducing oxidative injury induced by myocardial ischemia-reperfusion in rats. Methods：Sixty healthy male SD rats were randomly divided into 5 groups (n = 12): the sham operating group (Sham), the ischemia-reperfusion group (IR), the sevoflurane preconditioning group ( SPC), the sevoflurane preconditioning + 5-hydroxydecanoate group (SPC +5-HD)and the 5-hydroxydecanoate group (5-HD). Serum cTnI were detected at the end of reperfusion and myocardial infarct size(IS) were measured by triphenyltetrazolium chloride（TTC）staining. The Ca2 + levels and malondialdehyde (MDA) content in the myocardium were determined. The activity of superoxide dismutase (SOD) and glutathione S-transferase (GST) was measured. Western blot assay was used to assess the expression of glutathione S-transferase Mu (GSTM) expression in the left ventricle. Results： After ischemia-reperfusion,IR group indicated a significant increase of infarct size ,serum cTnI release, myocardial MDA and Ca2+ content, but a decreased activity of SOD, GST and myocardial GSTM expression (P＜0.01).SPC attenuated the infarct ratio and cTnI release, down-regulated myocardial MDA and Ca2+levels， but up-regulated SOD and GST activity and myocardial GSTM abundance(P＜0.01). As compared with SPC group, SPC+5-HD group increased infarct size, serum cTnI levels, myocardial MDA and Ca2+ content, but decreased SOD , GST activity and myocardial GSTM (P<0.01). Conclusion：Delayed preconditioning with sevoflurane can reduce oxidative injury induced by myocardial ischemia-reperfusion, in which mito-KATP is an important regulator.
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    Osteogenic differentiation of human bone marrow mesenchymal cells induced with Cbfa1 through non-viral vector
    2011, 39 (8):  743-746 . 
    Abstract ( 1289 )   PDF (652KB) ( 2067 )  
    Objective: To study the effect of core-binding factor a1 (Cbfa1) on the human bone marrow mesenchymal cells(hBMSCs) osteogenic differentiation. Methods: The hBMSCs were harvested through gradient centrifugation and adhering to the plastic culture wall. The third passage cells were used in our study. After the MSCs were infected with Cbfa1 through non-viral vector FuGene HD, the expressions of mRNA for osteoblast gene markers such as Cbfa1, alkaline phosphatase, osteocalcin, osteopontin and type I collagen were analyzed with real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR ) at 7d and 14d. Alkaline phosphatase ( ALP ) activity and expression of osteocalcin were measured at 1, 2, and 3 weeks after transfection. Results: The real-time RT-PCR indicated that all the osteoblast gene markers were up-regulated in the Cbfa1 treatment group. The morphous of hBMSCs modified with Cbfa1 were similar to osteoblasts, and expressed ALP and osteocalcin. Conclusion: FuGene HD-Cbfa1 can directly promote the osteogenic differentiation of hBMSCs.
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    短篇论著
    Clinical analysis of female infertility following tubal pregnancy after hysteroscopic and laparoscopic surgery.
    2011, 39 (8):  748-750 . 
    Abstract ( 1229 )   PDF (2131KB) ( 2262 )  
    Abstract Objective: To investigate the clinical characteristics and post-operation outcome in infertile women following tubal pregnancy. Methods: We retrospectively analyzed the data of consecutive in-hospital female infertility cases which the last pregnancy was tubal pregnancy and rule out male infertility from Dec 2008 to Aug 2009. Results: A total of 602 female infertility cases was admitted to Pingjin Hospital, Medical College of Chinese People’s Armed Policed Forces during this period. In these patients, infertility following tubal pregnancy accounted for 15.1% (91/602). All of them were managed with a combined interventional procedure (laparoscopy and hysteroscopy) in our department. Under laparoscopy the gross pathological changes of fallopian tubes involved normal appearance (20.7%), dropsy and atresia (23.3%), adhesions (59.3%), partial resection (10.7%), and other diseases of pelvic cavity (14.3%). After laparoscopic operation, infertility case with tubal blockage accounted for 63.3% under hysteroscopic tubal catheterization. The patency rate of blocked fallopian tubes after hysteroscopy directed guidewire tubal catheterization was 66.3%. Conclusion: previous tubal pregnancy which cause abnormal oviduct morphology and tubal blockage is the important reason of female infertility. Laparoscopy and hysteroscopy can advantage infertility women due to previous tubal pregnancy to resume reproductive.
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    Percutaneous transluminal angioplasty for the treatment ofchronic arterial occlusive disease below the knee
    2011, 39 (8):  750-751 . 
    Abstract ( 1353 )   PDF (1430KB) ( 2261 )  
    Abstract Objective: To evaluate the clinical effects of Percutaneous transluminal angioplasty(PTA) in the treatment of infrapopliteal arterial occlusive disease.Methods: The data of 39 patients(43limbs) who underwent infrapopliteal PTA from Jun 2008 to July 2009 in our hospital were reviewed.Results: Procedures were performed on 40 of 43 limbs(92%) successfully. During a mean follow up time of (8 range,3~18) months, ankle-brachial index (ABI) was increased from 0.41±0.12 before PTA to 0.82±0.05 after PTA（P<0.01）.There was no death in hospital The limb salvage rate and complication rate was 91.4% and 13%(6/43),respectively.Conclusion: Infrapopliteal PTA can be performed with favorable clinical results in patients with chronic occlusive disease and reduce the amputation rate.PTA should be considered as an effective and safe therapy for lower extremity ischemia caused by infrapopliteal occlusive disease.
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    Effect of DuIoxetine on treating depression with chronic pain
    2011, 39 (8):  756-757 . 
    Abstract ( 1714 )   PDF (1455KB) ( 2278 )  
    【Abstract】Objective: To study the effect and reliability of Duloxetine in the treatment of Depression with chronic pain. Methods: 60 cases of depressive disorders with pain were treated by Duloxetine for 8 weeks,at the same time，their emotion state,change of life quality,adverse effect,the remission of pain with HAMD、HAMA、WHOQOL、TESS、VAS and laboratory tests were evaluated.Results: After 8 weeks,patients’ scores of depression 、anxiety and pain decreased significantly from the baseline(p<0.01).Three domains of life quality (physiology，psychoLogy and independence) became much better than those of baseline(p<0.05). Conclusion: Duloxetine can reduce the depression、anxiety and pain ,improve the patients’ quality of Iife.
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    临床论丛
    Experience of 30 cases spleen tumor
    2011, 39 (8):  765-765 . 
    Abstract ( 748 )   PDF (715KB) ( 2168 )  
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    Morphological features of prostate intraepithelial neoplasia and its significance
    2011, 39 (8):  768-739 . 
    Abstract ( 1553 )   PDF (2223KB) ( 2238 )  
    【Abstract】 Objective To investigate the morpaology of prostate intraepithelial neoplasia(PIN) and its significance. Methods 103 specimen of prostate biopsy, including 26 cases with prostate carcinoma and 77 cases of benign prostate hyperplasia (BPH), were collected. Morphological features were reviewed. The expression of 34BE12, P63 and p504s were assayed in all patients by immunohistochemistry. Results High grade intraepithelial neoplasia(HGPIN) was found in 23 of 26 case with prostate carcinoma. There was no low grade intraepithelial neoplasia (LGPIN) to be observed in the patients above. Of 77 cases with BPH, intraepithelial neoplasia were found in 24 cases,(including 18 cases of LGPIN and 6 cases of HGPIN ). The histological types of HGPIN were mainly cluster and micropapilla structures (accounting for 92%). Immunohistochemistry: Among 29 cases with HGPIN , the discontinuous expression of 34βE12 was observed in basal cell around gland. In those of prostate carcinoma with HGPIN, P504s expression was from poor to moderate positive in the lesions of HGPIN near carcinoma(+-++), whereas was from moderate to strong expression in those away from carcinoma(++-+++); In the patients who were BPH with HGPIN, the positive expression was only observed in the two of 6 cases. The positive ratio of P504s was 82% in the case with HGPIN. Conclusion HGPIN is only precancerous lesion of prostate carcinoma. It must be differentiated from atypical hyperplasia of small alveolar gland and intraductocarcinoma of prostate; P504s is specific to HGPIN and carcinoma of prostate. The expression of it is probably associated with the progression of these tumors.; The correct diagnosis of HGPIN must depend on morphologic features and immunohistochemistry stain.
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