Table of Content

15 July 2010, Volume 38 Issue 7 Previous Issue    Next Issue
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论著
实验研究
短篇与病例报告
短篇论著
临床论丛


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论著

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The Correlation study of endometrial carcinoma and insulin//Ras/MAPK signaling pathway 2010, 38 (7):  545-548 .  Abstract ( 1531 )   PDF (629KB) ( 2132 )   Objective: To investigate the expression and correlation of Grb-2、Ras、P-ERK、CD-1，which are the main members of insulin/Ras/MAPK signal pathway in endometrial carcinoma. Methods:The expressions of Grb-2、Ras、P-ERK、CD-1 protein in tissues from 51 cases of endometrial carcinoma, 10 endometrial atypical hyperplasia, and 48 normal endometrium were detected by using immunohistochemistry Elivison method and image analysis system. The serum insulin concentration was measured by radioimmunoscintigraphy in endometrial carcinoma.Results:The positive expression rate of Grb2、Ras、P-ERK、CD-1 in normal endometrium、endometrial atypical hyperplasia and endometrial carcinoma was gradually increased. The expression rate of Grb2、Ras、P-ERK、CD-1 in endometrial carcinoma was higher than that in normal endometrium and endometrial atypical hyperplasia. In endometrial carcinoma there were positive correlation between Grb-2 and FIGO stage，either between P-ERK and FIGO stage (P<0.05). Also，there were positive correlation between Grb-2 and lymphonode metastasis(P＜0.05). There were positive correlations among the expression rates of Grb-2-Ras、Grb-2-P-ERK in endometrial carcinoma. The expression of Grb-2 and P-ERK was correlate with fasting serum insulin level in endometrial carcinoma（P＜0.05）.Conclusion:Insulin/Ras/MAPK pathway may play an important role in the carcinogenesis and development of endometrial cancer. Related Articles | Metrics

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The Study of function of endothelial progenitor cells from peripheril blood of newly diagnosed type 2 diabetes 2010, 38 (7):  549-551 .  Abstract ( 1401 )   PDF (627KB) ( 2048 )   Objective To study the vascular endothelial function and the quantity of endothelial progenitor cells（EPCs） from peripheril blood in the newly diagnosed type 2 diabetes(T2DM) and the related factors . Methods Endothelial-dependent vasodilation(EDV), The function of EPCs，High sensitive C reactive protein(hsCRP )， insulin active index(IAI)， blood glucose and blood-fat were measured in controls and newly diagnosed T2DM. Results The quantity of EPCs were significantly reduced in T2DM,EDV and IAI were lower in T2DM than that in Control group. Conclusions These findings suggest that the quantity of EPCs and endothelial function were decressed and these factors were closed related with IAI，hsCRP and the level of blood glucose. Related Articles | Metrics

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Molecular Characterization of Rubella Viruses Isolated in Tianjin 2010, 38 (7):  552-555 .  Abstract ( 1990 )   PDF (1031KB) ( 2378 )   Abstract Objective: To understand the genetic characteristics of rubella viruses in Tianjin in 2009.Methods: Rubella viruses were isolated by Vero/SLAM cells and identified through RT-PCR.1170 nucleotides of E1 gene were amplified and sequenced.The phylogenetic tree of 10 rubella strains compared to the WHO reference strains for each genotype based on the 739 nucleotides of E1 gene was performed.Results: The 10 strains detected from Tianjin formed a distinct cluster in genotype 1E in the phylogenetic tree.The nucleotide sequence identities are 97.3%-100%. There was no change in the important antigenic epitope sites. Only one amino acide change（Trp234→Ser234）in Tianjing09-06 at the hemagglutination inhibition and neut ralization epitopes.Meanwhile the 10 strains isolated from Tianjin had the same amino acide change（Leu338→Phe338）in E1 protein.The result sustained other doctor’s research.The amino acide （Phe338）may only existed in Chinese 1E genotype rubella viruses.Conclusion: The rubella virus genotype circulated in Tianjin is 1E.The 10 specimens in this study clustered three close together in our phylogenetic analysis,suggesting that there may be multi-transmission routes in Tianjin. Related Articles | Metrics

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Expressions of p15 and nm23 genes in uterine endometrial carcinoma 2010, 38 (7):  556-558 .  Abstract ( 1454 )   PDF (860KB) ( 2011 )   Objective To study the expressions of p15 and nm23 in human uterine endometrial carcinoma and to explore the role and clinical significance of the genes in the development of the disease. Methods The expression of p15 and nm23 in 60 specimens of uterine endometrial carcinoma was examined by immunohistochemistry S-P methods. Results the expression of p15 was associated with pathological grades (p<0.05). The expression of nm23 was associated with operation-pathological stages and lymph node metastasis (p<0.05).There was positive correlation between p15 expression and nm23 expression in the disease (P<0.01). Conclusions Low expression of p15 and nm23 may contribute to the development of uterine endometrial carcinoma, and the expressions of the genes in the malignant tumor is consistency. Related Articles | Metrics

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Study on the change of serum Cystatin C in the post kidney transplanted patients 2010, 38 (7):  582-583 .  Abstract ( 1648 )   PDF (1147KB) ( 2286 )   Study on the change of serum Cystatin C in the post kidney transplanted patients Xie lili, Liu juan, Gao qiang, Sun ping. Tianjin NO.1 centre hospital(300192) Abstract: Object To investigate diagnostic value of serum Cystatin C（Cys C） in the post kidney transplanted patients by dynamic monitoring the change and relationship among Cys C, sCr, BUN and Glomerular filtration rate (GFR). Method The serum Cys C, sCr and BUN were continuing measured in 29 patients during one day before operation and 1,3,5,7 day after operation compared with 20 controls. Particle-enhance turbidmetric immunoassay(PENIA) was used to detect the Cys C; sCr and BUN was measured by routine biochemistry assay; GFR was measured at by double plasma method. Result The value of the Cys C、sCr、BUN in the transplant group were higher than that in the control before operation, and all of them decreased at 1 day after kidney transplant operation, 47.6%, 37.4% ,33.0% respective, the level of GFR was negative correlated with Cys C(r=-0.892 P＜0.01), sCr(r=-0.811 P＜0.01) and BUN (r=-0.772 P＜0.01) .Conclusion The serum Cys C is a rapid, sensitive mark that could judge the kidney function after transplant operation. Key words: Cystatin C kidney transplant Creatinine BUN　Glomerular filtration rate Related Articles | Metrics

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Correlative Study between Shape of Chin and probability of Orthognathic Surgery for Class Ⅲ Adult Patients 2010, 38 (7):  587-589 .  Abstract ( 1504 )   PDF (1248KB) ( 2032 )   [Abstract] Objective： The purpose of this study was to investigate the correlation between shape of chin and probability of orthognathic surgery for class Ⅲ adult patients .To develop a discriminant method to predict probability of Clinical diagnosis and treatment. Methods： A total of 61 class Ⅲ adult patients were collected from the orthodontic department .thirty-one patients were treated by orthodontic appliance only, and others were treated with orthodontic and orthognathic surgery. Significant difference in age and sex distribution were not found between the two groups. Conventional Cephalometric analysis was made to compare hard and soft tissue structures before treatment between the two groups. The mean and standard deviation of each measurement were calculated for all the variables with SPSS. The correlation analysis, t-test and Discriminant Analysis were applied. Results： 1.Statistical analysis showed that the probability of orthognathic surgery is significantly correlated with B-Pg、Y、Co-Go、ODI、NSAr。2. The results showed that the B-Pg is closely correlated with MP/SN、MP/FH、MP/PP、L1/MP、ODI、ArGoGn in the orthognathic surgery group. Conclusion： The Shape of chin plays an important role in determining treatment plan about orthognathic surgery or orthodontic only for class Ⅲ adult patients. The probability of orthognathic surgery increases with the increasing in the degree of chin protrusion. Related Articles | Metrics

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Effect of Xuebijing Injection on the Systemic Inflammatory Response Syndrome of the Patients of Severe Acute Organophosphorus Poisoning Li Xiang Min 2010, 38 (7):  593-595 .  Abstract ( 2475 )   PDF (1290KB) ( 2270 )   Effect of Xuebijing Injection on the Systemic Inflammatory Response Syndrome of the Patients of Severe Acute Organophosphorus Poisoning （Li Xiangmin,Department of Emergency,Xiangya Hospital ,Central South University, Changsha 410008,Hunan,China） Abstract Objective The aim of this study was to explore the protective effect of Xuebijing Injection on the systemic inflammatory response syndrome (SIRS) of the patients of severe acute organophosphorus poisoning (AOPP) and its mechanisms. Methods 45 patients of severe acute organophosphorus poisoning were studied. They were randomly divided into two groups: the group treated with Xuebijing Injection (23 patients), and the routine treatment group (19 patients). In the former group, besides the routine treatment, the patients were treated with Xuebi Jing Injection additionally. The temperature (T), heart rate (HR), respiratory rate (RR), white blood cells (WBC), C-reactive protein (CRP), tumor necrosis factor (TNF-α), interleukin-6 (IL-6), and interleukin-8 (IL-8) of the patients before the treatment and on the 1st, 3rd and 5th days with the treatment were compared. Results After 3-day treatment, compared with the routine treatment group, the temperature, HR, RR, WBC and CRP of the patients in the group treated with Xuebijing Injection were significantly improved ( P< 0.01 or P< 0.05). The TNF-α、IL-6、IL-8 of them were significantly reduced (P<0.01). After 5-day treatment, the effects were more significant. Conclusions Xuebijing Injection can effectively inhibit the systemic inflammatory response syndrome of the patients of severe acute organophosphorus poisoning, significantly improve the symptoms of SIRS, and it might play a very important part in helping treat the AOPP patients. Related Articles | Metrics

实验研究

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The Study of RAW264.7 Cells Differentiate into Osteoclasts in Vitro 2010, 38 (7):  596-598 .  Abstract ( 1921 )   PDF (1423KB) ( 2393 )   Abstract Objective:To observe the biologic characteristics of RAW264.7 cells,and to validate the feasibility of RANKL-mediated differentiation of RAW264.7 cells into bone-resorptive Osteoclasts (OCs).Methods：RAW264.7 cells were treated with RANKL. RANKL-induced formation into OCs were observed after tartrate resistant acid phosphatase(TRAP) staining. Bone resorption pits were detected by scanning electron microscope. Results：RANKL-differentiated RAW264.7 cell cultures develop both TRAP+ mononuclear and multinucleated cells.It has the ability of generating bone resorptive pits. Conclusions：RANKL can induce RAW264.7 cells to mature OCs. RAW264.7 cells are easier to obtain and culture.So RAW264.7 cells can be used as a preferable preosteoclast model.This methods has good repeatability and feasibility.It represent a relatively homogeneous population of pre-OCs. Related Articles | Metrics

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Expression of MICA at the mRNA and protein levels in Human Gastric Epithelial cell Strain-1 of Hypoxia 2010, 38 (7):  599-601 .  Abstract ( 1422 )   PDF (1388KB) ( 2056 )   【Abstract】Objective :To investigate the effect of hypoxia on MICA expression in GES-1 its mechanism. Methods :Hypoxia culture model in vitro was established to test the expression of MICA in GES-1 cell line. After cultured in hypoxia circumstance for a given period of time (12hours ,24hours ,72hours ) ,MICA expression was tested in mRNA level and protein level by RT - PCR and immunocytochemistry analysis . Results: Cell survival was affected a little within 20 hours under hypoxia. MICA expressions increased after treatment of hypoxia. MICA protein expressions increased equally with mRNA confirmed by immunocytochemistry and RT - PCR and its positive correlation was found by Pearson product - moment correlation analysis. Conclusion :MICA gene and protein can be induced remarkably byhypoxia in GES-1. Related Articles | Metrics

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Effects of propofol on embryonic- and α7- nicotinic acetylcholine receptors 2010, 38 (7):  602-604 .  Abstract ( 1352 )   PDF (1413KB) ( 2130 )   Objective: This study was designed to examine the effects of propofol on embryonic- and α7- nicotinic acetylcholine receptors with whole cell clamp patch. Methods: The embryonic- and α7-nicotinic acetylcholine receptors were heterologously expressed in HEK293 cells and activated to maximal currents by the application of acetylcholine (100μmol/l). Peak currents were recorded in HEK293 cells treated by propofol using whole cell patch clamp technique. Results: Propofol produced rapid and readily reversible, concentration-dependent inhibition. The potence of propofol is greater to embryonic-nicotinic acetylcholine receptor compared to α7-nicotinic acetylcholien receptor(P<0.05), Propofol wasn’t result in dose-dependent inhibition of the embryonic- and α7- nicotinic acetylcholine receptors(P>0.05). Conclusion: the interactions of combination of muscle relaxants with dissimilar structure, rocuronium and atracurium, were synergistic to both adult- and embryonic- nicotinic acetylcholine receptors and with similar structure, rocuronium and vecuronium, were synergistic to adult- nicotinic acetylcholine receptors and additive to embryonic- nicotinic acetylcholine receptors. Related Articles | Metrics

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Culture and identification of human umbilical vein endothelial cells in vitro 2010, 38 (7):  605-607 .  Abstract ( 1109 )   PDF (1406KB) ( 2327 )   ABSTRACT Objective: To establish a effective method to isolate, culture and identify from human umbilical vein endothelial cells ( HUVEC) in vitro. Methods: Trypsin perfusion method was used to isolate HUVEC from fresh umbilical cord, and then the cells were cultivated in M199 with 20 % fetal bovine serum. After the collected endothelial cells were cultured and passaged, the cells were observed under inverted phase contrast microscope. The endothelial specific surface marker were assessed by fluorescence activated cell sorter ( FACS) analysis. And the cells were also identified by the test of up take of acety-lated low density lipoprotein (Ac-LDL). Results: The obtained HUVECs were small-round and clustering. The cells began adhere to the plate in tow hours, and attached cells were cord-like structure. After 7days culture the cells grow into large number and can be confluens a monolayer, which displayed cobble-stone morphology. These cells express endothelial specific markers, including VE-cadherin, vWF and CD31.The cells’function were identified by up take of Ac-LDL. Conclusion: A large number of high purified endothelial cells could be acquired by digestion of trypsin. HUVEC can be proliferated and passaged successfully in vitro. Related Articles | Metrics

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Animal Experimental Study of Oral Ulcer by Compound Epidermal Growth Factor And Glycyrrhizic Zinc Pellicles 2010, 38 (7):  608-609 .  Abstract ( 1880 )   PDF (916KB) ( 2108 )   Objective: To investigate the efficacy of oral ulcer animal model by compound epidermal growth factor and glycyrrhizic zinc pellicles. Methods: The 24 rabbits were randomly divided into treatment group, control group and blank control group, all 8,burning 1 min on the rabbits right mouth mucosa with wet acetic acid cotton.Physiological saline wash 5 min, ulcers were formated after 24h. Anesthetized rabbits At 8:00, 12:00, 16:00 every day, The treatment group using the compound pellicles treated 2h,the control group using ulcer paste treated 2h, the control group no treatment,treatment for 5d.Daily measurement of maximum diameter of ulcers in rabbits,comparison between groups observed. Results: When treatment to the 3rd day, the treatment group was more effective than the control group and blank group（P＜0.05）, there was no significant difference between the control group and blank group（P＞0.05）;when treatment to the 5th day,there was no significant difference between the control group and treatment group（P＞0.05）, but the two groups were all more effective than blank group（P＜0.05）.(2)Rabbits in the appearance of cure, organization of the control group of rabbits has been repaired epithelium, but thinner than normal tissue,lamina fiber was loose than normal tissue; epithelial surface incomplete keratinizated, was basically consistent with the normal tissue.Conclusions: The exact effect of compound epidermal growth factor and glycyrrhizic zinc pellicles can be effective for treatment of oral ulcer ainimal model. Related Articles | Metrics

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Influence of Insulin Resistance on Activity of PI-3-K in Platelet 2010, 38 (7):  610-612 .  Abstract ( 1550 )   PDF (1398KB) ( 2035 )   Abstract Objective: To study the influence of insulin resistance on activity of PI3-K in platelet. Methods: 24 beagle dogs were randomly divided into control group and test group .The animal's diet was normal in control group and high-fat in test group to establish the animal models of insulin resistance. Normal blood sugar–high insulin clamp test was performed after six months to confirm the animal models of insulin resistance. The PI3-K activity, aggregation rate and adhension rate of platelet were measured. Results: PI3-K activity of test group is higher than normal control group (P<0.05) and platelet adhesion rate, aggregation rate were increased in test group(P<0.001). Conclusion: Insulin resistance increased platelet PI3-K activity, which was the key to enhanced platelet activity. Related Articles | Metrics

短篇与病例报告

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Detecting the Correlation between the Systolic Peak Velocity of Middle Cerebral Arteries(MCA-PSV) and Pregnant Durations by Doppler Ultrasonograpy 2010, 38 (7):  612-612 .  Abstract ( 1923 )   PDF (468KB) ( 2115 )   Related Articles | Metrics

短篇论著

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The clinical observation of emergency PCI therapy within the IABP combined tirofiban through venous 2010, 38 (7):  616-617 .  Abstract ( 1506 )   PDF (972KB) ( 2090 )   Objective To observation the efficacy of emergency PCI therapy within the IABP combined tirofiban through venous in acute myocardial infarction (AMI) merger cardiogenic shock (CS) patients. Methods 26 patients suffered acute myocardial infarction (AMI) merger heart shock (CS), we did emergency PCI therapy supported by the IABP combined tirofiban through venous, and the patients’ tolerance to operation , hemodynamics, cardiac function, thrombus and bleeding complications , mortality were observed. Results Except two cases died of large area of infarction and pump failure, 24 cases were finished the operation of PCI and stent implantation in infarction related artery (IRA), the success ratio is 92.31%. The average time from disease begin to vascular recanalization is 8.13±2.82h; another three postoperative patients died in ICU because cardiac function worse, the total hospitalization mortality is 19.23%. There is no limb artery embolization and deadly bleeding complications occurred (such as intracerebral hemorrhage, internal bleeding, etc.). The hemodynamics of survival cases was obvious improvement than before (P < 0.05), and the cardiac function get better compared with admission (P < 0.05). Conclusion The emergency PCI therapy within the IABP combined tirofiban through venous in acute myocardial infarction (AMI) merger cardiogenic shock (CS) patients, which can greatly improve patients’ tolerance to operation, improve the hemodynamics and cardiac function, reduce the complications and mortality. Related Articles | Metrics

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The application of transesophageal echocardiography to patients undergoing cardiac valve surgery 2010, 38 (7):  619-620 .  Abstract ( 1922 )   PDF (969KB) ( 2200 )   Abstract Objective：To assess the clinic value of transesophageal echocardiography(TEE)during cardiac valve surgery．Method：To monitor 247 patients undergoing cardiac valve surgery in order to compare the results with pre-operative conditions and observe retained air.Result： (1)Additional defect was found in 29 cases(11.74％).Pre-operative diagnosis was found to be wrong by TEE in 20 cases(8.09％).(2)There was significant improvement in the mitral regurgitation grade after mitral replacement(P<0.01).Significant residual mitral regurgitaton(＞grade3) that necessitated immediately further surgery was identified in 5(2.5%） patients.The results for re-do were satisfactory.(3)The left atrial gas was detected in 188(76.11%) patients among 247 cases.Conclusion:Intra- operative transesophageal echocardiography is useful in supplementing pre-operative diagnosises and assessing immediate operative results or identifying retained air. Related Articles | Metrics

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Reasonable Application of FK506 after Liver Transplantation in Infants 2010, 38 (7):  621-622 .  Abstract ( 2295 )   PDF (986KB) ( 2306 )   Objective: To investigate the best application and the ideal blood concentration of FK506 after liver transplantation in infants. Methods: From September 2006 to November 2009,13 infants received liver transplantation in Center of East Organ Transplantation, First Central Hospital of Tianjin, including 8 living donor liver transplantations and 5 split liver transplantations. Corticosteroids were applied intra-operatively, and the FK506-based immunosuppressive therapy was used after the operation. The dosage and blood concentration of FK506 were monitored within 3 months, 3 months to 1 year, 1 year postoperatively, except for the FK506-related complications. Results: All of the patients were followed up 3 to 40 months (median 18 months), 11 cases of them were still alive, 2 cases died. The blood concentrations of FK506 within 3 months, 3 months to 1 year, 1 year after operation were （8.64±3.71）ng?ml-1,（7.90±2.76）ng?ml-1 and（5.38±1.73）ng?ml-1, while the dosages were (0.21 ± 0.12) mg ? kg-1 ? d-1, (0.18 ± 0.08) mg ? kg-1 ? d-1 and (0.11 ± 0.03) mg ? kg-1 ? d-1. The FK506-related complications postoperatively included acute rejection in 2 cases, infections in 12 man-times, FK506-related psychotic symptoms in three cases. Conclusion: The blood concentration of FK506 after liver transplantation in infants should be maintained at the appropriate level, and the dosage adjustments should be based on the concentration of FK506, so as to minimize the side effects of FK506 caused by insufficient or excessive amount. Related Articles | Metrics

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Analysis on Karyotypes of Villus from 897 Advanced Maternal Ages during the First Trimester of Gestation 2010, 38 (7):  622-623 .  Abstract ( 1581 )   PDF (1476KB) ( 2012 )   Objective: To evaluate the risk of abnomal fetus through analysis on karyotypes of advanced maternal age. Methods: Villus chromosome karyotype analysis was performed for 897 pregnant women. Results: 64 abnormal chromosomal karyotypes and 54 de novo abnormal chromosomal karyotypes were detected by prenatal diagnosis, including 14 sex chromosome abnormalities, 27 autosome chromosome number abnormalities, and 13 autosome chromosome structural abnomalities, 10 polyploidy. Conclusion: The risk of the fetus with chromosome abnormalities in the pregnant women aged over 35 years was high and chromosome karyotype analysis was necessary and effective in prenatal diagnosis. Related Articles | Metrics

临床论丛

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The relationship between portal vein pressure and portal vein thrombosis in patients after splenectomy for portal hypertension 2010, 38 (7):  629-630 .  Abstract ( 1616 )   PDF (1071KB) ( 2084 )   Abstract Objective: To analyze if there are relationships between the portal vein pressure preoperation(PVP)/descendent level of portal vein pressure postoperation(DPVP) and portal vein thrombosis in patients after splenectomy for portal hypertension due to cirrhosis resulting from hepatitis. Methods: The clinical data of 226 patients with hypertension due to cirrhosis resulting from hepatitis receiving simple splenectomy or splenectomy and portal-azygous devascularization in our hospital from August 2000 to June 2007 were retrospectively analyzed. The effective cases were 154. the cases were divided into 2 groups according to with or without portal vein thrombosis, relation of portal vein thrombosis to PVP and DPVP was analyzed. Accord to the DPVP, the cases were divided into ≤8cmH2O and >8cmH2O 2 groups, and we analyzed the rate of portal vein thrombosis between the 2 groups. Average comprision was studied by student-t test or nonparametric test, rate comprision was studied by chi-square test. Results: The portal vein thrombosis occurred in 31 patients and 123 patients had no thrombosis occurred. Our analysis showed that the PVP of the group with thrombosis was higher than the group without thrombosis, the DPVP of the group with thrombosis was higher than the group without thrombosis(P<0.05). The rate of thrombosis occurrence in the DPVP>8cmH2O group was greater than in the DPVP≤8cmH2O group. Conclusion: The PVP and the DPVP are important factors to portal vein thrombosis in patients after splenectomy for portal hypertension due to cirrhosis resulting from hepatitis. The higher the DPVP was, the greater odds of the portal vein thrombosis occur. Related Articles | Metrics

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Difference in CD8+T cell activation of prepheral blood between acute and chronic EB virus infection children 2010, 38 (7):  630-631 .  Abstract ( 1904 )   PDF (1068KB) ( 2023 )   Abstract Objective:To analyze the difference in total T cell, CD8+T cell and HLA-DR expression on CD8+T cell of peripheral blood in 27 children with acute EB virus infection and 7 children with chronic EB virus infection.Results: Total T cell , CD8+T cell and CD8+/HLA-DR+ cell in acute infected group were higher than chronic infected group and control(p<0.01);T cell was lower than control and CD8+/HLA-DR+ cell was higher than control in chronic infected group(p<0.01).Conclusion: There were significant difference in total T cell, CD8+T cell and HLA-DR expression on CD8+T cell of peripheral blood between acute and chronic EB virus infection ,It was low activated level of CD8+T cell in chronic EB virus infection.It can evaluated cellular immune function to detecte CD8+T cell activation for EB virus infected children. Related Articles | Metrics

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The clinical study of median effective dose of ropivacaine for lumbar plexus blockade in elderly patients 2010, 38 (7):  631-632 .  Abstract ( 1298 )   PDF (1044KB) ( 2090 )   The clinical study of median effective dose of ropivacaine for lumbar plexus blockade in elderly patients MA Lin,YangYong, YU Yong-hao Department of Anesthesiology, General Hospital, Tianjin Medical University, Tianjin 300052, China Abstract Objective： To determine the median effective dose（ED50） of ropivacaine for lumbar plexus blockade in elderly patients. Methods：Forty patients ,aged 65～85(n=20) or 18～35(n=20) underwent lumbar plexus and sciatic nerve blockade for operations of unilateral lower extremities. The volume of local anesthetic used in each patient was based on the up-and-down method for lumbar plexus blockade. The ratio of two consecutive dose was 1.12. Successful nerve block was defined as complete loss of pinprick sensation in the femoral nerve distribution , with concomitant inability to extend the affected leg within 30 min after local anesthetic injection. Results：The ED50 of 0.375% ropivacaine was 28.84 ml in the older group and 34.28 ml in the younger group （P<0.05）.The 95% confidence interval was 27.42～30.45 ml in the older group and 32.24～35.61 ml in the younger group. Conclusion：The ED50 of ropivacaine for lumbar plexus blockade is significant decrease in elderly patients. Related Articles | Metrics