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15 August 2010, Volume 38 Issue 8 Previous Issue    Next Issue
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临床论丛
论著
短篇论著
实验研究
论著
短篇论著
论著
综述与讲座
实验研究
论著
实验研究
检验技术
临床论丛


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临床论丛

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The chemotherapy regimen based on carmustinum and cytarabine for the treatment of NHL in stage Ⅳ 2010, 38 (8):  0-0 .  Abstract ( 1251 )   PDF (980KB) ( 2011 )   To detect the cost-effective chemotherapeutic regimen of NHL in stage Ⅳ. Methods We treated the patients with carmustinum (62.5-125mg/d，d1), cytarabine (100mg/d，d1-5),Pirarubicin （40mg/d，d1）、vincristine (1.5mg/d，d1 and d8) and dexamethasone (10mg/d, d1-14). All the patients were diagnosed NHL and in stage Ⅳ. Before chemotherapy we monitored the functions of their heart and liver and kidney and the hyperplasia degree of bone marrow. All the index above were normal. Results During all the 22 patients were identified, 5 patients obtained CR, 10 attained PR and the effective rate was 68.2%. Conclusions The chemotherapeutic regimen based on carmustinum and cytarabineis one of the cost-effective ways to treat the patients with NHL in stage Ⅳ. Related Articles | Metrics

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Anesthesia Management of One Child with Larsen Syndrome 2010, 38 (8):  0-0 .  Abstract ( 646 )   PDF (1020KB) ( 1965 )   Related Articles | Metrics

论著

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Relationships of true insulin and proinsulin to Body fat distribution in patients with obstructive sleep apnea-hypopnea syndrome 2010, 38 (8):  0-0 .  Abstract ( 876 )   PDF (760KB) ( 1781 )   objective: To study the relationship between serum true insulin(TI), proinsulin(PI) and their body fat distribution in patients with obstructive sleep apnea-hypopnea syndrome(OSAHS). Methods: 72 diagnosed cases were divided into three groups,mild group,moderate group and severe group according to apnea hypopea index(AHI),meanwhile 15 healthy people were selected as control group. the serum TI,PI and body mass index(BMI),waist circumference(WC),neck circumference(NC) were detected. Results: The levels of PI and TI were higher in OSAHS group than healthy group,and there were statistically significant differences among four groups.pearson analysis indicated that the levels of TI and PI were positively correlated with BMI,WC and NC in OSAHS patients.Conclusion: Both TI and PI in the serum were correlated to Body fat distribution in OSAHS patient. Related Articles | Metrics

短篇论著

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Assessed correlation of the length of intravesical prostatic protrusion with the clinical complication in patients with benign prostatic hyperplasia 2010, 38 (8):  0-0 .  Abstract ( 1793 )   PDF (915KB) ( 1888 )   Abstract：Objective: We assessed correlation of intravesical prostatic protrusion（IPP） with the clinical symptoms in patients with benign prostatic hyperplasia(BPH).Methods: We retrospectively assessed 218 BPH patients from November 2007 to November 2009 in our hospital，IPP was measured by transabdominal ultrasound, accordance with Tan YH’s methods,the length of protrusion was classified as three group: IPP I 1-5mm or less,IPP II greater than 5 to 10mm and IPP III greater than 10mm.We assessed correlation of the length of IPP with clinical symptoms in patients with BPH.Results: IPP was found positively correlated with the prostate volume(PV), prostate-specific antigen(PSA), postvoid residual urin volume(PVR)and bladder out obstruction index(BOOI)(Spearman's rho=0.497，0.346，0.334，0.659，p <0.01）, negatively correlated with the peak urinary flow rate(Qmax)（Spearman's rho =-0.139, p < 0.05). PV was found statistically different between the per group（P<0.01）, PSA、Qmax、PVR and BOOI were found statistically different between IPP I and III（P < 0.05）,and between IPP II and III（P < 0.05）. Incidence of complication are increase with the step up of the group, were 40.82%，58.62%，68.47%,respectively. And the incidence was found statistically different between the per group（P=0.004）.There are 49 patients with serious complication,32 patients in IPP III(65.31%),10 in IPP II(20.41%)and 7 in IPP I(14.28%). The incidence of patients, who selected surgery ,are found statistically different between the per group,were 67.35%、89.66%、97.30%,respectively（ P＜0.01).Conclusion:There is a close correlation between the length of IPP with the voluminal and micturitional parameters of BPH,and between the degree of IPP with the incidence and severity of complication.Length of IPP could applied to reference of therapeutics. Related Articles | Metrics

实验研究

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Experimental Study of Slow-Release Stent of Heparin in Myocardial Revascularizition after Acute Myocardial Infarction 2010, 38 (8):  0-0 .  Abstract ( 1119 )   PDF (1257KB) ( 1810 )   Aim To investigate the effect of slow-release stent of heparin implantation into transmural channels on myocardial revascularizition after acute myocardial infarction. Methods After the mid third of left anterior descending (LAD) was ligated, miniswine were grouped into control group (A group, n=6), transmyocardial drilling revascularization (TMDR) group (B group, n=6), TMDR+stent implantation group (C group, n=6). In B and C groups, two transmural channels with 3.5 mm in diameter were established by using a self-made drilling device, followed by implantation of two stents into the channels in C group. Vascular density, myocardial perfusion, histological and morphological analyses, and left ventricular (LV) function were evaluated at pre-treatment and 6 weeks post-treatment. Results At 6 weeks post-treatment, vascular density was significantly increased in C group (OD =4268±331 pixels/hpf), compared with A group (2557±241 pixels/hpf, P<0.001) and B group (2412±221 pixels/hpf, P<0.001). Changes of Mass defect percent (△MDP) significantly decreased in B group (-1.43±0.39%), compared to A group (2.83±0.31%, P<0.001) and B group (2.54±0.27%, P<0.001). There were more viable cardiaomyocytes in C group (OD=68224±4190 pixels/hpf) than that in A group (36345±3714 pixels/hpf, P<0.001) and in B group (41138±3989 pixels/hpf, P=0.003). LV ejection fraction (EF) significantly increased in C group (56±3%), relative to A group (49±2%, P=0.037) and B group (48±2%, P=0.019). There were no significant differences in these parameters between A group and B group. Conclusions Slow-release stent of heparin implanted into transmural channel could keep the channel open and promote luminal endothelialization by continuous anticoagulation, promoted angiogenesis, increased myocardial perfusion, enhanced myocardial viability, improved LV function, may be a new method for myocardial revascularization of acute myocardial infarction. Related Articles | Metrics

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Inhibition of Osteoclast Formation by hOCIL Extracellular Domain 2010, 38 (8):  0-0 .  Abstract ( 1373 )   PDF (2193KB) ( 1821 )   [Abstract] Objectives:Osteoclasst inhibitory lectin (OCIL) expressed by the osteoblast/stomal cells is a type-Ⅱ transmembrane protein. OCIL extracellular domain was obtained to investigate its effect on the formation of osteoclast like cells. Mehtods: based on the codon preference and degeneration, the codons of OCIL extracellular domain was changed into those preferred by E.coli coding and ligated the fragments with recombinant PCR. By induction of IPTG, the recombinant cells BL21(DE3)plysS with transfection of pMAL-c2x/hOCIL produced the soluble recombinant protein in the cell lysates. then the combination activity between maltose binding protein (MBP) and Amylose was used to purify the fusion protein. The biological action of the recombinant protein was analyzed by its effect on osteoclast formation in murine bone marrow cell culture under the stimulation of macrophage-colony stimulating factor (M-CSF) and receptor activator of nucleic factor κB ligand (RANKL). Results: osteoclast like cells (OLCs) formation was significantly blocked by the recombinant protein. Conclusion: the research successfully obtained the recombinant protein hOCIL from E.coli with biological activity that may inhibited the osteoclast like cells formation in vitro. Related Articles | Metrics

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Heat Shock Preconditioning on Pancreatic Graft Reduces Posttransplant Ischemia Reperfusion Injury of Rat 2010, 38 (8):  0-0 .  Abstract ( 1670 )   PDF (1341KB) ( 1847 )   Abstract Objective：Rats were heat shock preconditioned to determine the mechanism that heat shock precondition(HPC) reduce ischemia reperfusion injury of rats model after pancreas transplantation. Method：Expression of heat shock protein70 (HSP70)of pancreas of rats who were heat shock preconditioned was detected at different time. rats model of ischemia reperfusion injury after pancreas transplantation were set up. Donator rats of high expression of HSP70 were regarded as experiment group for pancreas transplantation and donator rats of non- heat shock precondition as control group.Blood and pancreas of rats were examined after pancreas transplantation after 6 hours.Expression of HSP70 in pancreas was examined with western-blotting. Expression of TNF-α in pancreas was detected with immunohistochemistry. Hemodiastase of rats was determined with iodoamylum chromatometry.Pathological changes of pancreas was detected with microscope. Result：Expression of HSP70 of pancreas of donator rats achieves peak after HPC 12 hours, which has significant difference comparison with other time points （P<0.05）, and which has significant difference comparison with no precondition group（P<0.01）,recovery after HPC 48 hours. Levels of TNF-α、neutrophil and hemodiastase of pancreatic tissue significantly increased in control group comprared with that in sham group（P<0.01）。Levels of TNF-α、neutrophil and hemodiastase of pancreatic tissue significantly decreased in experiment group comprared with that in control group（P<0.05）,and interstitial edema and hemorrhage of pancreas were reduced in experiment group. Conclusion：Heat shock preconditioning reduce ischemia reperfusion injury of rats model after pancreas transplantation. Mechanism of delaying protective effect after HPC correlate to production of HSP70. Related Articles | Metrics

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Expression of estrogen receptor α and β in childhood acute lymphoblastic leukemia and clinical significance 2010, 38 (8):  0-0 .  Abstract ( 1383 )   PDF (1107KB) ( 1831 )   [Abstract] Objective To investigate the expression and relation of estrogen receptor(ER) α and β in childhood acute lymphoblastic leukemia(ALL). Methods: Fist using an immunocyto- chemical stain technique APAAP to immunophenotype thirty childhood with ALL in newly diagnosed group.The expression of ERα and ERβ in all patients’ mononuclear cells of bone marrow was detected by S-P immunohistochemical assay.The control group including 15 children with normal bone marrow. Results:The positive expression of ERα and ERβ is 56.7% and 76.7% respectively in the newly diagnosed ALL group, both were remarkably significant higher than that in the control group (P<0.05). The rate of the remission have no significant difference between the positive expression and negative expression of ERα(P>0.05). The rate of the remission of the positive expression of ERβ was remarkably significant higher than that of the negative expression of ERβ(P<0.05). The rate of the positive expression of ERα have no significant difference between the remission and non-remission group(P>0.05). The rate of the positive expression of ERβ in the remission group was remarkably significant higher than that in the non-remission group (P<0.05).The expression of ERα and ERβ were no difference respectively in ALL-B and ALL-T. Conclusions: Childhood ALL may be an incretion dependent tumor. ERβ may play an important role in the occurrence, development and prognosis of childhood ALL. Related Articles | Metrics

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The expression and clinical significance of retinoic acid receptors αβ in ovarian tissues 2010, 38 (8):  0-0 .  Abstract ( 1038 )   PDF (903KB) ( 1740 )   [Abstract] Objective：To explore the expression and clinical significance of retinoic acid receptors αβ in normal and abnormal ovarian epithelial cancer tissues．Methods：Expression of retinoic acid receptors was detected in 20 normaltissues, 20 benign tissues ,20 bordline tissues and 52 ovarian cancer tissues using immunohistochemistry．Results：The positive expression of retinoic acid receptorα in normal tissues , benignin tissues , bordline tissues and ovarian cancer patients were 80.77% 、50% 、15%、20% respectively,it has statistical significance in different pathological grade and FIGO stage. The positive expression of retinoic acid receptorβ in normal tissues , benignin tissues , bordline tissues and ovarian cancer patients were 76.92% 、45% 、30%、30% respectively．The expression of retinoic acid receptorβwere related to the histological type ,FIGO stage (P<0.05)． Conclusion：The expression of retinoic acid receptorαβwere higher in ovarian cancer ,and may be associated with ovarian carcinogenesis． Related Articles | Metrics

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The value of Carotid intima-media thickness and diameter in the diagnosis of atherosclerosis 2010, 38 (8):  0-0 .  Abstract ( 1062 )   PDF (957KB) ( 2007 )   ABSTRACT：　Objective　To evaluate the relationship between common carotid artery (CCA) intima-media thickness (IMT),common carotid artery diameter(D) and carotid artery atherosclerosis(CAS) by ultrasonograph . Investigated IMT and D as a sensitivity indicator of the CAS,and to provide an early prevention for cerebral infarction. . Methods　 The carotid artery of 40 high-risk group and the 40 mild dangerous group with hypertension were assessed by color Doppler ultrasonography. IMT and D were measured at the common carotid arteries .Results ①The IMT, D of hypertension the high-risk group are higher than the mild dangerous group obviously（P <0.05 ）；②There was a significant difference in abnormal rate of isolated IMT or D between hypertension high-risk group and mild dangerous group（P <0.05 ），but there was a extremely significant difference with the abnormality both IMT and D to compare（P <0.01） . Conclusion The enlargement of common carotid artery IMT and D as a objective maker to CAS, but coexistent abnormalities of IMT and D are more sensitive to CAS. Related Articles | Metrics

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The correlation between thromboxane A2 receptor gene polymorphism and susceptibility of asthma 2010, 38 (8):  0-0 .  Abstract ( 1298 )   PDF (1104KB) ( 1837 )   【Abstract】Objective To determine whether the thromboxane A2 receptor gene T924C polymorphism is associated with the hereditary susceptibility of children asthma as well as the change in lung function and serum specific IgE . Methods This study recruited 125 asthmatic children who were suffering from acute episode and 49 healthy children as control group. Polymerase chain reactions- Restriction fragment length polymorphism (PCR-RELP) techniques were used to detect the TBXA2R SNP. In asthmatic group, serum concentrations of specific IgE antibodies were measured by Allergyscreen. All asthmatic patients who were more than five years old underwent spirometric assessment. Results The distribution frequencies of genotypes (TT,TC,CC) in asthmatic patients were 67,52,6, and the corresponding figures in controls were 38,7,4, there is significant difference between two groups. The frequencies for wild-type(T) and mutant alleles (C) in T924C in our asthmatic patients were 0.744 and 0.256 respectively, and the corresponding figures in controls were 0.8469 and 0.1531. The C alleles frequencies in asthmatic patients are obviously higher than in controls, the difference is significant. The heterozygous genotype(TC) can significantly increase the risk of developing asthma, the OR value (95%CI) is 4.213 (1.741-10.196), p =0.001. The asthmatic patients were divided in two groups (≥3 years of age and＜3 years of age),Compare the genotypes and alleles frequencies in two grups , there is no significant difference. Compare the different items of lung function between TT group and TC+CC group using t-test, there is no statistically significance. Compare the genotypes(TT,TC+CC) distribution and alleles frequencies between sensitization to universal allergens(house dust、house dust mites 、fungi、cat、dog )and no sensitization to them , the p values are all more than 0.05, there is no statistically significance. Conclusion TBXA2R gene T924C polymorphism is associated with the increased genetic susceptibility of developing asthma,the C allele may be susceptibility gene for asthma.The distribution of genotypes doesn’t change with age. The heterozygous genotype (TC) can increase the risk of developing asthma. There is no association between TBXA2R T924C polymorphism and change in lung function or serum sIgE in asthmatic children. Related Articles | Metrics

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Application of Carotid Arteries Ultrasound in High Cardiovascular Risk Patients. 2010, 38 (8):  0-0 .  Abstract ( 1051 )   PDF (1478KB) ( 2111 )   【Abstract】 Objective To observe the relationship between carotid arteries ultrasound and cardiovascular risk factors,clinical cardiovascu1ar events as well as the degree of coronary artery disease in high cardiovascular risk patients.Methods Relevant clinical data, including height, weight, BMI, blood pressure, heart rate, history and blood biochemistry test were collected in 131consecutive patients with high cardiovascular risk and needed to be proformed selective coronary angiography(CAG). The patients were divided into 4 groups by Intima-media thickness(IMT) and degree of carotid plague through carotid arteries ultrasound exam. Plaque is defined as a focal structure that encroaches into the arterial lumen ,IMT value or demonstrates a thickness >1.5 mm as measured from the media-adventitia interface to the intima-lumen interface. The number of risk factors and cardiovascular events in four groups were compared.Results Compared within four groups,the cardiovascular events and the number of cardiovascular risk factors were highest in the fourth group (0.00±0.00;0.29±0.47; 0.46±0.66;0.58±0.65; P<0.05and2.35±0.61;3.14±1.35;3.46±1.33;3.75±1.07P<0.05,respectively).Systolic blood pressure are significant difference in four groups. With diffused carotid artery plaque to predict three vessel coronary artery disease,the sensitivity and specificity is 70.37% and 96.77% respectively.Conclusions The patients with higher grade-level carotid artery plaque have high risk of cardiovascular diseases, and cerebrovascular events as wel as higher incidence of cardiovascular risk factors. Application of carotid artery ultrasound classification may be can predict coronary artery disease. 【Key words】carotid arteries ultrasound;cardiovascular risk factors;cardiovascular events;coronary atherosclerosis Related Articles | Metrics

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Study on the association of the matrix metalloproteinase-9 gene -1562C/T polymorphism with diabetic nephropathy 2010, 38 (8):  0-0 .  Abstract ( 1725 )   PDF (880KB) ( 1821 )   Abstract Objective: To explore the dependability of matrix metalloproteinase-9 (MMP-9) gene -1562C/T and diabetic nephropathy (DN), and the relationship with different stage of DN. Methods: 150 type 2 diabetes (T2DM) patients were divided into DN group and diabetes without DN (NDN) group. DN group included microamount albuminuria, clinical alubuminuria and renal inadequacy stage. 52 healthy people were selected as normal control (NC) group. The genotypes were assessed by restriction fragment length polymorphism．Results: The average HbA1c、SBP、Cr、BUN、UAER of DN group were significantly higher than that of NDN or NC group (P<0.01).There was significant difference in frequencies of allele or genotype of polymorphismC/T between three groups (P<0.01). DN incidence rate in type 2 diabetics containing with genotype of CC, CT or TT respectively were decreased gradually. DN incidence rate containing with allele C and T were 59.2% and 40.5% respectively. The T allele carriers had 0.47 times risk for increased incidence of DN compared with that of C allele carriers (P<0.01，95%CI 0.29～0.75). The frequencies of TT genotype and T allele in three stages of DN decrease with renal hypofunction. Conclusion: The polymorphism of MMP-9 -1562C/T is associated with the development of DN, T allele is the protective gene of DN. Related Articles | Metrics

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Effects of high glucose on angiopioetin-like protein 3 expression in vitro and in vivo 2010, 38 (8):  0-0 .  Abstract ( 1195 )   PDF (524KB) ( 1733 )   Abstract:Objective To observe the effects of high glucose on angiopioetin-like protein 3(ANGPTL3) expression in vivo and in vitro .To explore the relationship and of ANGPTL3 with the diabetes mellitus （DM）. Methods Take human serum and L02 as research subjects including 10 cases healthy human serum as control group（male : famale=5:5）and 10 cases of hyperglycemia serum as experimental group (M:F=5:5) from clinic. L02 cells were cultured separately in mediums containing 5.6，7.1，11.2 and 20mmol/L glucose，among which 5．6 mmol／L glucose group was taken as contro1；The relative level of ANGPTL3 protein in serum was measured by ELISA .The relative level of ANGPTL3 mRNA expression was detected by reverse transcription-polymerase chain reaction(RT-PCR). The relative level of ANGPTL3 protein in L02 was measured by Western Blot. Results The concentration of ANGPTL3 protein in hyperglycemia group was obviously higher than that of ctrol group(P<0.05).High concentration of glucose in medium could stimulate the expresion of ANGPTL3 mRNA and protein in cultured L02 cells . The differences were significant (P<0.05).Conclusion high glucose can upregulate ANGPTL3 expression both in vtro and in vivo. The expression of ANGPTL3 might by related to DM. Related Articles | Metrics

短篇论著

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Approach of swell and necrosis in local skin and parenchyma by Chinese cobra snakebite 2010, 38 (8):  0-0 .  Abstract ( 1662 )   PDF (977KB) ( 1825 )   【Abstract】 Objective To approach the effective therapy of the swell and necrosis in local skin and parenchyma by Chinese cobra snakebite. Methods The clinical data of 67 patients those all were bitten by Chinese cobra, were divided into three groups and were paved sulfadiazine silver,40%magnesium sulfate glycerol and jidesheng snake tablet respectively. Detumescence time and healing time were compared and analyzed. Results The mean detumescence time of group sulfadiazine silver,40%magnesium sulfate glycerol and jidesheng snake tablet were （8.82±1.92）days、（10.35±2.10）days and（11.42±2.61）days respectively, there were difference of statistical significance in the three groups (p<0.05).Compared by each two groups, there were both difference of statistical significance between the sulfadiazine silver group to the 40%magnesium sulfate glycerol group and the jidesheng snake tablet group (p<0.05),but there were no difference of statistical significance between the 40%magnesium sulfate glycerol group and the jidesheng snake tablet group (p>0.05).The mean healing time of group sulfadiazine silver,40%magnesium sulfate glycerol and jidesheng snake tablet were （17.45±6.67）days、（19.92±7.63）days and（20.74±8.50）days respectively. There were no difference of statistical significance in the three groups (p>0.05). Conclusions Treating the swell and necrosis in local skin and parenchyma by Chinese cobra snakebite, the efficacy of sulfadiazine silver was better than the 40%magnesium sulfate glycerol and jidesheng snake tablet at detumescence time, but there were no good and bad at healing time. Related Articles | Metrics

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A Comparison of the Clinical Efficiency of Continuous Wave of Condensation and Cold Lateral Compaction Obturation Techniques 2010, 38 (8):  0-0 .  Abstract ( 1319 )   PDF (1002KB) ( 1992 )   [Abstract] Objective： To evaluate the efficiency of root canal obturation using the continuous wave of condensation technique. Methods： A total of 94 patients receiving root canal therapy in the maxillary central incisors were divided equally into two groups. The control group received cold lateral compaction obturation therapy, and the experimental group was treated with continuous wave of condensation technique. Manipulation time and apical extrusion for each group was determined. Data were statistically analysed using Independent t-test or Chi-square test in SPSS v11.5. Results ：Continuous wave obturation was significantly faster than cold lateral compaction (P < 0.01), but there was no significant difference in the length of root canal obturation. Conclusion： The continuous wave technique was more efficient than cold lateral compaction. Related Articles | Metrics

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The Analysis of the size of adenoidal tissues of subjects with unilateral cleft lip and palate REN Zhong Yu 2010, 38 (8):  0-0 .  Abstract ( 1225 )   PDF (1258KB) ( 1885 )   Objective: Adenoidal hypertrophy is a considerable causation of maxillofacial abnormalities. The purpose of this study was to compare dimensionally the sizes of the adenoidal tissues and in juvenile and adolescent people with and without cleft lip and palate (CLP), to observe the feature speciality of the people with cleft lip, providing theory reference for the clinical diagnosis and treatment reference design theory of the malocclusion with cleft lip. Methods: The subjects were selected from 85 patients with malocclusion， 43 patients with CLP; 42 patients without CLP . An analysis of variance was used to test for significant differences in mean values . Results: Adolescents with CLP compared with control group: the size of adenoid is obviously increasing. Juveniles with CLP compared with control group: the size of adenoid has no obviously difference. Adolescents with CLP compared with juveniles with CLP: the size of adenoid is obviously increasing .Conclusions: CLP juveniles compared with control group: the thickness of adenoid、the area of adenoid、the ratio of thickness of adenoid and the width of pharyngonasal cavity are obviously increasing . The larger adenoidal tissues in the CLP/j group, compared with those in the control/j group, decreased to a similar size with aging. Related Articles | Metrics

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Advances in the studies of Programmed death-1(PD-1) on chronic viral hepatitis 2010, 38 (8):  0-0 .  Abstract ( 1191 )   PDF (1932KB) ( 1826 )   Abstract: Hepatitis B virus (HBV) and hepatitis C virus (HCV) infection is the main cause of chronic active hepatitis, cirrhosis and hepatocellular carcinoma. Recent studies have shown that the negative regulating virus-specific reactive T-cell of programmed death-1(PD-1) is a decisive factor of HBV and HCV hepatitis virus persistent infection so that is play an important role in chronic viral (HBV and HCV) hepatitis. Here is a reviewe about the recent researches on the programmed death-1(PD-1) in chronic viral(HBV and HCV) hepatitis. Related Articles | Metrics

实验研究

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The effect of Jinqi hypoglycemic table on expression of SREBP-1cmRNA in the liver of insulin-resistant rats 2010, 38 (8):  0-0 .  Abstract ( 1245 )   PDF (1694KB) ( 1942 )   The effect of Jinqi hypoglycemic table on expression of SREBP-1cmRNA in the liver of insulin-resistant rats SUN Nan，CUI Jing-qiu，GAO Zhi-hong, FENG Ping, The General Hospital Of Tianjin Medical University，Tianjin 300052，China 【Abstract】Objective To investigate the efficacy of Jinqi hypolgycemic tablet on expression of SREBP-1c mRNA in the liver of insulin-resistant rats. Methods Established a rat model of insulin resistance (IR) by feeding high-fat diet for 8 weeks, then randomly divided the insulin-resistant rats into two groups: HF group (n＝9) that was fed with high-fat diet for another 6 weeks,，HF＋JQ group (n＝9) that was fed with high-fat diet and Jinqi hypolgycemic tablet for 6 weeks. Measured blood glucose, serum insulin, lipid concentrations and serum leptin, serum TNF-α. Semi-quantitative RT-PCR was used to detect the expression of SREBP-1c mRNA in the liver of rats at the end point. Results Compared with the HF group, serum insulin, OGTT 2h glucose, serum lipid, serum leptin and TNF-αconcentrations in HF＋JQ group were decreased obviously, and insulin sensitive index（ISI）was higher. In addition, , the expression of SREBP-lc in the liver of HF＋JQ group was significantly reduced compared with HF group[(0.75±0.06 vs 0.92±0.05)，P<0.01]. Conclusion Jinqi hypolgycemic tablet can inhibit the expression of SREBP-1c mRNA in the liver of high-fat-fed rats and improve insulin resistance . 【Key words】 Insulin resistance, SREBP－1c, leptin, TNF-α, Hypoglycemic drugs (traditional Chinese medicine Related Articles | Metrics

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Combination of NF-κB Inhibitor and Docetaxel Enhances Anti-tumor Activity in Anaplastic Thyroid Cancer Cell Xenograft in Vivo 2010, 38 (8):  0-0 .  Abstract ( 1249 )   PDF (1193KB) ( 1933 )   Objective: We sought to study if Dehydroxymethylepoxyquinomicin (DHMEQ), a novel NF-κB inhibitor, can enhance docetaxel’s anti-tumor activity in anaplastic thyroid cancer (ATC) cells (FRO) xenograft in nude mice test. Methods: FRO xenograft nu/nu mice models were created by injecting 5×106 cells on both flanks. The dosages of drugs were determined as intraperitoneal (i.p.) injection with 5 mg/kg of docetaxel once a week; daily i.p. injection with 6mg/kg of DHMEQ for two weeks; combined treatment was given with both drugs, and control group mice received vehicle injections only. One day after treatment, TUNEL apoptotic staining was performed on excised tumor tissues. Tumor dimensions were monitored for 32 days. Results: Although any drug was able to delay tumor growth, the combined treatment of DHMEQ and docetaxel for two weeks was much more effective. The TUNEL apoptotic staining showed drug-treatment induced many positively stained cells, while the combined regiment tremendously increased positively stained cells. Conclusion: For the first time the treatment of docetaxel combined with NF-κB inhibitor were tried on ATC-cell xenograft in vivo models. Docetaxel is able to induce NF-κB signaling pathway in cancer cells, which could attenuate anti-tumor activities of the drugs, but NF-κB inhibitor can effectively suppress this phenomenon and create chemo-sensitive environment and synergistically enhance apoptosis. Related Articles | Metrics

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The analysis of results of hTERC gene expressed before and after treatment in cervical intraepithelial neoplasia 2010, 38 (8):  0-0 .  Abstract ( 558 )   PDF (935KB) ( 1753 )   Related Articles | Metrics

实验研究

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The Compare of L-type Calcium Current in Ventricular Myocytes between Healthy and Diabetic Rabbits 2010, 38 (8):  0-0 .  Abstract ( 1549 )   PDF (1336KB) ( 1810 )   Objective: To compare L-type calcium current in ventricular myocytes between heathy and diabetic Rabbits with patch clamp.Method:To make the diabetes model with tetraoxypyrimidine,There were 4 groups:nomal control group,nomal ischemia/reperfusion group,diabetes control group and diabetes ischemia/reperfusion group.To record the current at basic state and after ischemia/reperfusion.Result:There were statistic significant differences of ischemia/reperfusion(p<0.01) and time(p<0.01),and not of diabetes.There were not interaction between ischemia/reperfusion ,diabetes and time,except between ischemia/reperfusion and time(p<0.01).The slope rate of diabetes group was less than that of nomal on Time-Density line.Conclusion:Diabetes didn’t make significant differences in ICa,L,but minify the attenuation degree after ischemia/reperfusion ,wich might provide some clue for the effect of diabetes on ischemia preconditioning. Related Articles | Metrics

检验技术

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DETECTION ADULT'S REFERENCE RANGE OF RETICULOCYTE PARAMETERS BY SYSMEX XT-2000i HEMATOLOGY ANALYZER 2010, 38 (8):  0-0 .  Abstract ( 1970 )   PDF (1267KB) ( 1878 )   [Abstract] Objective To establish reference range of reticulocyte counts in healthy adults of Tianjin by Sysmex XT-2000i hematology analyzer. Methods 6 parameters of reticulocyte were evaluated on 102 healthy adults(52men&50women) by Sysmex XT-2000i hematology analyzer and the normal reference range were determined by statistic analysis. Results RET%: male is 0.888±0.247,famale is 0.850±0.277,there is no significant difference between each group(P>0.05),unite reference range of RET% is 0.34-1.40.RET#(×1012/L):male is 0.04396±0.01243,famale is 0.03836±0.01295,there is significant difference between each group(P<0.05),reference range of male is 0.0161-0.0658,famale is 0.0125-0.0643.LFR%:male is 98.86±0.85,famale is 96.16±0.87,there is no significant difference between each group(P>0.05),unite reference range of LFR% is 97.3-100.MFR%:male is 0.66±0.64,famale is 0.46±0.62,there is no significant difference between each group(P>0.05),unite reference range of MFR% is 0-1.82.HFR%:male is 0.48±0.59,famale is 0.48±0.59,there is no significant difference between each group(P>0.05),unite reference range of HFR% is 0-1.5.IRF%:male is 1.14±0.85,famale is 0.84±0.87,there is no significant difference between each group(P>0.05),unite reference range of IRF% is 0-2.7. Conclusion There are differences between reference range of reticulocyte parameters with sex,analyzer,area and so on. Reference range of reticulocyte parameters should be established according to laboratory itself analyzer. Related Articles | Metrics

临床论丛

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Clinical analyse of 110 cases of cervical intraepithelial neoplasia 2010, 38 (8):  0-0 .  Abstract ( 1528 )   PDF (938KB) ( 2001 )   Abstract:Objectives To approach reasonable treatment mehods by analyzing clinical cases of cervical intraepithelial neoplasia Ⅲ(CINⅢ).Methods 110 cases of CINⅢ enrolled in Tianjin Central Hospital of Obstetrics and Gynecology from Jan 2005 to Dec 2006 were analyzed .Results Of the 110 patients,87 cases underwent conization,8 hysterectomy;15 extralfascial hysterectomy.18 patients accepted secondary abdominal surgey(4 extrafascial hysterectomy;10 secondary extensive hysterectomy and lymphadenectomy; 4extensive hysterectomy and lymphadenectomy).Ex -trafascial hysterectomy was adminietrated on one patients with carcinoma in situ of cervix(CIS) and uterine myoma.The patho-staging was Ⅰa2,and radiotherapy was added.Conclusions Three-step examination is the standard procedure.Conization is the first sletion of CINⅢ.Bleeding and cervical canal stenosis are the common complications.Patients of stage Ia1 with negative edge after conization and endocervical curettage should be fllowed closely. Related Articles | Metrics

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Diameter and thickness measurement of normal children lens by B-mode ultrasonography 2010, 38 (8):  0-0 .  Abstract ( 1785 )   PDF (1063KB) ( 1851 )   Objective Diameter and thickness measurement of children lens of different ages by B-mode ultrasonography explore the relationship between age and lens development. Methods Diameter and thickness of the lens in 398 children were measured by B-mode ultrasonography and then statistically analyzed. Results After statistical processing, lens thickness and diameter were positively correlated with age; lens thickness were positively correlated with diameter; Diameter and thickness of lens increased with age in each age group. Following conclusions were achieved by interval prediction: the lens thickness in children were close to adults from 2.5 years to 3 years; the diameter of lens in children were close to adults from 11 years to 14 years; the thickness of children lens were close to adult’s when the diameter of lens were from 8.1mm to 8.2mm. 〔normal lens diameter of adult was 9-10mm, normal lens thickness of adult was 4-5mm〕. The diameter and thickness of lens were in rapid growth period under 3 years old and in slow growth period after three. The difference between men and women had no statistical significance; the difference between right and left eyes had no statistical significance. Conclusion Children with intraocular lens implantation should choose the appropriate age and appropriate intraocular lens. Related Articles | Metrics