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15 January 2011, Volume 39 Issue 1 Previous Issue    Next Issue
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论著
心血管疾病研究
实验研究
短篇论著
临床论丛
短篇论著
临床论丛


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论著

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ADHESION MOLECULE-1 IN DIFFERENT OBESITY STANDARD WOMEN WITH POLYCYSTIC OVARIAN SYNDROME 2011, 39 (1):  1-3 .  Abstract ( 1565 )   PDF (2216KB) ( 2275 )   【Abstract】 Objective To compare the level of soluble intercellular adhesion molecule-1(sICAM-1) in different obesity standard women with polycystic ovary syndrome (PCOS), approaching the value of treatment and diagnose of different obesity standard in PCOS. Methods Selected 54 women with PCOS for experimental group, According to BMI≥24kg/m2 or（and）WHR≥0.8, all the subjects were respectively divided into obese A subgroup （BMI≥24kg/m2）、obese B subgroup（WHR≥0.8）、obese C subgroup （WHR≥0.8 and BMI≥24kg/m2）and non-obese A subgroup（BMI<24kg/m2）、non-obese B subgroup（WHR<0.8） 、non-obese C subgroup（BMI＜24kg/m2 and WHR＜0.8）.Then compared the level of sICAM-1 between different obese subgroup and non-obese subgroup. Results 1.Serum sICAM-1 level in obese A subgroup was higher than non-obese A subgroup (P<0.05);2. Serum sICAM-1 level in obese B subgroup was higher than non-obese B subgroup (P<0.05);3. Serum sICAM-1 level in obese C subgroup was higher than non-obese C subgroup (P<0.05); 4. There was no significant deviation in serum sICAM-1 level between the patients with obese A subgroup and obese B subgroup and obese C subgroup (P>0.05);5. Serum sICAM-1 level in non-obese A subgroup was higher than non-obese B subgroup and non-obese C subgroup (P<0.05); There was no significant deviation in serum sICAM-1 level between the patients with non-obese B subgroup and non-obese C subgroup (P>0.05); 6. Serum sICAM-1 level had positive correlation with BMI,WHR(P<0.05),the dependablity between Serum sICAM-1 level and WHR exceeded the dependability between Serum sICAM-1 level and BMI. Conclusion Our study showed that the serum sICAM-1 level of PCOS patients is correlated to obesity, the affection of WHR ≥0.8 central obesity in sICAM-1 is even bigger, To hint: PCOS patients with central obesity possible to appear cardiovascular diseases. So we must control WHR to decrease the expression of sICAM-1, in order to prevent cardiovascular diseases. Related Articles | Metrics

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RELATIONSHIP BETWEEN ATRIAL FIBRILLATION AND THE IL-6- 174G/C、-572C/G、-597G/A POLYMORPHISM 2011, 39 (1):  4-6 .  Abstract ( 1457 )   PDF (2314KB) ( 2381 )   Abstract Objective: To explore the relationships among atrial fibrillation (AF) , Interleukin-6 (IL-6) and single nucleotide polymorphisms (SNP) of the IL-6 -174G/C,-572C/G and -597G/A. Methods: The serum levels of interleukin -6 (IL-6), lipid and other clinical indicators were measured. The left atrial diameter (LAD) were measured by echocardiography (UCG). The frequency distribution of genotypes and alleles of the IL-6 -174G/C,-572G/C and -597G/A in samples of 127 AF patients were amplified by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Results: IL-6 level and LAD of AF group is significantly higher than that of control group(P<0.01). The C allele mutation rate of IL-6 -174G/C is minimal in Han population in Jiangsu Nantong, and IL-6 -597G/A has no A allele mutation in the experiment. IL-6 -572C/G polymorphisms exist in Han population in Jiangsu Nantong, the G/C+G/G genotype distribution and G allele frequencies of IL-6 -572C/G between Atrial fibrillation group and control group have significant differences (P<0.01). Conclusion: IL-6 level and the pathogenesis of AF may be have some relationships. This study shows that among Han population in Nantong IL-6 -572 C/G polymorphic allele G mutation in the AF group is significantly higher than that of the control group (P<0.01), which may be associated with the inflammation and the pathogenesis of AF. Related Articles | Metrics

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Study on the expression and the significance of zn and IL-2、IL-10 in serum and E2F1 in organization of all kinds cervical diseases patients’ with high-risk HPV 2011, 39 (1):  7-9 .  Abstract ( 2261 )   PDF (3612KB) ( 2481 )   Abstract：Through objective the zinc 、 IL-2、IL-10 content in serum and E2F1 in organization of cervical disease patients with the high-risk HPV infection,investigate it’s Clinical significance.Methods :Selected 130 cervical disease patients , including 30 CIN Ⅰ、30 CIN Ⅱ、20CIN Ⅲ and 20 cervical carcinoma with high-risk HPV infection, 30chronic cervicitis with HPV infection negetive. Detect Zn by Atomic absorption spectrometry and detected IL-2、IL-10 in their serum by ELISA. The expressions of E2F1 were detected by immunohistochemistry. Results:The content of Serum zinc、IL-2，in the CIN Ⅱ、CIN Ⅲand cervical cancer were decrease were all significantly(p<0.05);the content of IL-10，in the CIN Ⅲand cervical cancer were decrease were all significantly(p<0.05); the compare of the expression of E2F1 ，in the chronic cervicitis、the CIN and cervical cancer were all significantly(p<0.005).IL-2’s levels and zinc’s in serum was positively correlated（p<0.05), ,IL-2’s levels and IL-10 in serum was negatively correlated（p<0.05）, IL-2’s levels and E2F1’s in serum was negatively correlated（p<0.05 ）. Conclusion:In the high-level cervical disease and cervical cancer , occurred the drift of Thl to Th2, impact the clear of HPV . Zinc deficiency involved in this change. . Related Articles | Metrics

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The growth inhibitory effect of OGF on human breast carcinoma cells in vitro enhong xing 2011, 39 (1):  10-13 .  Abstract ( 1683 )   PDF (4374KB) ( 2449 )   Abstract Objective：To investigate the growth inhibitory effect of OGF and the opioid antagonist naloxone on MCF-7 human breast carcinoma cells. Methods：Cell counting、MTT essays and cell cycle distribution analysis by flow cytometry were used to detect the inhibiting growth effect of OGF on MCF- 7 cells. Results: Cell counting showed that the cell concentration reduced markedly in OGF group than other groups（P<0.01）. No difference was found between naloxone and control groups（P>0.05）. MTT essays indicated that the inhibiting rate of cell growth of OGF group was significantly higher than that in naloxone and OGF/naloxone groups (P<0.01). There was no significant difference among naloxone, OGF/ naloxone and control groups（P>0.05）. Flow cytometry results showed that PI was lower in OGF group than in other groups（P<0.01）, and no significant difference was found between naloxone and control groups（P>0.05）.Conclusions: OGF could inhibit the growth of MCF-7 cells in vitro and the growth inhibitory effect of OGF can be blocked by naloxone. The cell cycle was obstructed by OGF in G0/G1 phase. Related Articles | Metrics

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The comparative analysis of axillary small incision and traditional posterolateral incision in the lung cancer operation 2011, 39 (1):  17-20 .  Abstract ( 1616 )   PDF (3022KB) ( 2792 )   Abstract Objective:To search for the clinical value of axillary small incision in the lung cancer surgery. Methods:From January 2009 to January 2010,151 surgery patients with stageⅠ-Ⅲ lung cancer were collected and divided into the axillary incision group (80 cases) and traditional posterolateral incision group (71 cases),The perioperative indicators and last day before operation or 4 hours,1 day,2 day after operation of serum CRP and cytokines (IL-6, IL-8) concentration were compared. Results:The axillary incision group in the intraoperative blood lose ,incision length, postoperative bed stay, drainage volume, drainage tube retention, postoperative hospital stay, postoperative pain intensity score, postoperative dosage of morphine, postoperative complications were all significantly lower than traditional posterolateral incision group (P <0.05).Compared with last day before operation , CRP was significantly increased at 4 hours,1 day,2 days after operation in both groups,and the peak value at 1 day after operation. Compared with last day before operation , IL-6 and IL-8 were significantly increased at 4 hours,1 day,2 days after operation in both groups,and the peak value at 4 hours after operation. The serum concentration of CRP and cytokines (IL-6, IL-8) at 4 hours,1 day,2 days after operation in the axillary incision group were all significantly lower than traditional posterolateral incision group (P <0.05). Conclusion:Compared with the traditional posterolateral incision, the axillary incision has lighter acute phase inflammatory reaction,less body damage, less pain, faster recovery and so on，axillary small incision can be completely meet the requirements of all kinds of pneumonoresection and systematic lymph node dissection, can be used as routine incision of lung cancer surgery. Related Articles | Metrics

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The evaluation of left ventricular diastolic function by Velocity Vector Imaging 2011, 39 (1):  21-23 .  Abstract ( 1639 )   PDF (3569KB) ( 2331 )   【Abstract】 Purpose To explore the feasibility of Velocity Vector Imaging（VVI） in evaluation of left ventricular diastolic function. Methods Choosed 61 patients of left ventricular diastolic dysfunction and 40 subjects as the normal control group, every patient were examined by VVI work station. 4 mitral annular sites: anterior and inferior from apical 2-chamber views，lateral and septal from apical 4-chamber views were adopted. The early and late diastolic velocity (Ve, Va), the early and late diastolic strain rate (SRe, SRa) were measured, the Ve/Va, SRe/SRa ratio and the mean value of 4 sites were calculated. Results The Ve, SRe, Ve/Va , SRe/SRa of the patients with diastolic dysfunction were significantly decreased compared with the control group in every mitral annular site and Va, SRa were significantly increased（P<0.001）. Conclusion The motion of mitral annular could be measured quantitily by VVI and VVI could be used to evaluate the left ventricular diastolic function as a new method. Related Articles | Metrics

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Patients with hypertension risk factors of cardiovascular events 2011, 39 (1):  24-26 .  Abstract ( 2234 )   PDF (2202KB) ( 6322 )   Objective: To explore the related risk factors of cardiovascular events in patients with hypertension. Methods: Clinical data from 507 patients with hypertension who hospitalized in Tianjin Chest Hospital from Jan 2007 to Dec 2009 were analyzed focusing on the risk factors of cardiovascular events. We analyze the indicators between patients who occurred with and without cardiovascular events. We used Logistic regression to analyze the related factors in order to explore the risk factors of cardiovascular events. Results: Among the 507 patients with hypertension, 288 were male and 219 were female. The incidence of cardiovascular events is 46.15%. The incidence is 70.09% in male and 29.91% in female. The independent risk factors of cardiovascular events were age, sex, BMI, LDL-C and fibrinogen. Conclusions: For patients with hypertension, fibrinogen is another independent risk factor for cardiovascular events besides traditional risk factors. We should pay more attention to it. Related Articles | Metrics

心血管疾病研究

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Relationship of Inflammatory cytokines gene polymorphism with restenosis after percutaneous coronary intervention in Tianjin population 2011, 39 (1):  27-30 .  Abstract ( 1137 )   PDF (4297KB) ( 2276 )   【Abstract】 Objective To investigate the relationship of inflammatory cytokines gene polymorphism with restenosis after percutaneous coronary Intervention(PCI) in Tianjin Chinese-Han population. Methods 437 patients who successfully underwent PCI with a follow-up angiography were divided into a restenosis group (166) and non-restenosis group (271). IL-6 polymorphism (-572C/G)、IL-1β polymorphism (-511C/T) and IL-10 polymorphism (-592C/A) in promoter region were genotyped respectively by restricted fragment length polymorphism using polymerase chain reaction (PCR-RFLP) method. Results (1) There were no significant difference in frequencies of IL-6-572 、IL-1β-511 and IL-10-592 genotype and allele between 2 groups (P>0.05); (2) Logistic regression analysis revealed postive correlations between Lp(a)、ACS patients、pre-PCI degree of stenosis、length of target lesion stenosis and restenosis （OR=1.90、3.92、2.07、3.04, respectively）; and there were negative correlations between the stent diameter, the diameter of reference vessel before stent implantation and restenosis（OR=0.76、0.39, respectively）. Conclusion (1)The polymorphism of IL-6 -572 C/G、IL-1β-511 C/T and IL-10 -592 C/A gene was not associated with restenosis in the Tianjin Chinese-Han population Related Articles | Metrics

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The Clinical Observation of Atorvastatin Combining Probucol to Improve Contrast-induced Acute Kidney Injury 2011, 39 (1):  31-34 .  Abstract ( 3559 )   PDF (2855KB) ( 2735 )   Abstract Objective To observe the improvement on contrast induced acute kidney injury (CIAKI) before coronary angiography with different doses of atorvastatin combined with probucol. Methods 98 cases admitted for coronary angiography (CAG) or percutaneous coronary stent implantation(PCI) were randomly divided into three groups：Standard treatment group: atorvastatin 20mg qn and Probucol 0.25g/tid, no enhancement before angiography; Strengthen and combined treatment group: atorvastatin 40mg qn and Probucol 0.25g/tid, atorvastatin 40mg and Probucol 0.5g 2 hours were administered before the angiography; Intensive therapy group: atorvastatin 40mg qn, atorvastatin 40mg 2 hours were administered before the angiography. All patients were then evaluated 24 hours before and after the angiography procedure, and their blood urea nitrogen (BUN), serum creatinine (Scr),renin All items (renin ,AngII and aldosterone), and estimated glomerular filtration rate (eGFR) by MDRD method were tested. Results 1) The Scr of Standard treatment group was higher postoperatively than the preoperative value (P = 0.044), e GFR decreased significantly (P = 0.006); The BUN of Strengthen and combined treatment group (P <0.001) and Intensive therapy group (P = 0.029) were remarkably decreased postoperatively. There was no significant difference in Scr and e GFR. (2) The value of △ Scr (P = 0.02)and△ AngII (P = 0.04) in Strengthen and combined treatment group was higher , △ e GFR (P = 0.015) was reduced.（3）For the patients of mild to moderate renal function , The value of△ Scr (P = 0.025) and △ AngII (P = 0.031) in Strengthen and combined treatment group significantly increased , △ eGFR (P = 0.025) was reduced .Conclusion The combination of atorvastatin 40mg and Probucol 0.25 tid or one with atorvastatin 40mg before angiography could improve the CIAKI, however, the role of Strengthen and combined treatment group improved significantly. For the patients of mild to moderate renal function, Strengthen and combined treatment group could improve significantly. Related Articles | Metrics

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The Experimental Study on the Inhibitory Effects of Aspirin on the Proliferation of Human Umbilical Artery Smooth Muscle Cells through Transforming Growth Factor β1 Lin WANG 2011, 39 (1):  35-37 .  Abstract ( 1475 )   PDF (2177KB) ( 2339 )   Objective: To investigate potential mechanisms of aspirin in inhibition of Human umbilical artery smooth cells (HUASMCs) proliferation through TGF-β1 signaling. Methods: HUASMCs were isolated and cultured in vitro incubated with or without specific anti–TGF-β1 neutralizing antibody. Cell proliferation and cell cycles were measured by MTT and flow cytometry after treated with different levels of aspirin. Results: The proliferation of HUASMCs were inhibited by aspirin（2.0、5.0 mmol/L）in vitro (P <0.05), independent of cytotoxicity. The anti-proliferation effects can be reversed by specific anti-TGF-β1 neutralizing antibody(P<0.05). Aspirin（2.0 mmol/L）restrained cells in G0 phase (P<0.05)，which can be reversed by specific anti-TGF-β1 neutralizing antibody(P <0.05). Conclusion: Aspirin, independent of its cytotoxicity, can inhibit the proliferation of HUASMCs in vitro, in which TGF-β1 signaling might be involved. Related Articles | Metrics

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Correlation between serum Lectin-like Oxidized Low-density Lipoprotein Receptor-1 and Acute Coronary Syndrome 2011, 39 (1):  38-40 .  Abstract ( 1382 )   PDF (2130KB) ( 2258 )   [Abstract] 　Objective　To investigate the correlation between serum level of lectin-like oxidized low-density lipoprotein receptor-1(LOX-1) and acute coronary syndrome (ACS ). Method There are 121 patients with coronary heart diseases (CHD) confirmed by CAG including 75 ACS patients,46 SAP patients,and 50 healthy subjects as the control group. Every sample’s serum LOX-1 level is detected by ELISA method. Results　On admission, the concentration of LOX-1 in ACS, SAP and control group is (216.38±40.67) 、(181.51±34.66)、(154.43±27.41)μg/L respectively；There is remarkable statistical difference between ACS and SAP group,and so is it between SAP and control group.There is no statistical difference of the level of LOX-1 between immediately after PCI and that before PCI. 1 week after PCI，the level of LOX-1 is lower than that before PCI， but there is no statistical difference between them. Conclusions The serum concentration of LOX-1 is higher obviously in ACS patients,and it is related to the stability of coronary plaque ,which shows LOX-1 may serve as a potential marker of plaque instability and could be the important monitering markers for the severity of ACS. Related Articles | Metrics

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Angioplasty in patients with acute myocardial infarction assisted by intra-aortic balloon counterpulsation 2011, 39 (1):  41-43 .  Abstract ( 960 )   PDF (2133KB) ( 2278 )   Related Articles | Metrics

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Comparison of safety between Yinyi micropole drug coated stents and China made sirolimus-eluting stents for treating patients with acute myocardial infarction 2011, 39 (1):  44-46 .  Abstract ( 2316 )   PDF (2198KB) ( 2298 )   【Abstract】 Objective To investigate the safety of Yinyi micropole drug coated stent after percutaneous coronary intervention (PCI) in patients with acute myocardial infarction．Methods From Oct.2008 to Jan．2010,consecutive patients with AMI underwent PCI were divided into Yinyi micropole drug coated stents group(Group A n=100)and PARTNER stents group(Group B n=90)according to the type of the stents．The incidence of major adverse cardiovascular event(MACE) and stent thrombosis were evaluated during in-hospital 、30days and 6 months follow up after PCI．Results One hundred and ninety patients were successfully treated by PCI .NO in-stent thrombosis was diagnosed in the group A ,while one case stent thrombosis in group B（0 versus 1.1%, P>0.05）.The rates of MACE did not differ significantly between the groups with Yinyi micropole drug coated stents and those PARTNER stents during in-hospital 、30days and 6 months follow up after PCI. Conclusion Implantation of Yinyi micropole drug coated stent after PCI is as safe as PARTNER stents in patients with AMI. Related Articles | Metrics

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Effect of platelet micropartiles on the expression of cell adhesion moleculein in cultured human umbilical endothelial cells 2011, 39 (1):  47-49 .  Abstract ( 1568 )   PDF (4822KB) ( 2274 )   Abstract objective: To examine the expressions of cell adhesion molecule(E-selectin.VCAM-1.ICAM-1) in HUVECs(CRL-1730),which is affected by platelet microparticles(PMPs).To investigate the effects of platelet microparticles in coronary heart disease.Methods: HUVECs(CRL-1730) and prepared PMPs were co-cultured.Reverse transcription-polymerase chain reaction(SQRT-PCR) was used to detect the relative expressions of E-selectin、 I CAM-1 and VCAM-1 in endothelial cells respectively.Results: The expressed levels of E-selectin、ICAM-1和VCAM-1 were increased when HUVEC(CRL-1730) were interfered by PMPs of centain concentration（p<0.05）.But the PMPs stimulated HUVECs(CRL-1730) at different times,the expressions of E-selectin、ICAM-1and VCAM-1 were of no diffeence（p>0.05）.Conclusions: The PMPs may increase the expressions of E-selectin、ICAM-1and VCAM-1 on HUVEC(CRL-1730).It may explain a possible mechanism of PMPs in coronary heart disease. Related Articles | Metrics

实验研究

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Effects of repetitive transcranial magnetic stimulation on the expression differences of c-FOS and NGF in the hippocampus of temporal lobe epilepsy rats 2011, 39 (1):  50-52 .  Abstract ( 1628 )   PDF (4757KB) ( 2243 )   Objective To study the effects of low-frequency repetitive transcranial magnetic stimulation (rTMS) on the expression of NGF and c-FOS in the hippocampus of temporal lobe epilepsy (TLE) rats. Methods Lithium-pilocarpine induced TLE rat models were randomly divided into TLE group and rTMS group. Control group consist of normal rats which were littermate with the other two groups. TMS was performed at 0.5 Hz, 0.4T for 0.2ms and 20 times per day for one week on rTMS group rats. TLE group and control group rats were treated with sonic stimulation as pseudo stimuli for the same times as rTMS group. With immunohistochemical technique, we investigated the differences expression of c-Fos and NGF in the hippocampus among the three groups. Results c-Fos and NGF was positively expressed in the hippocampus of all groups. c-Fos expression level of TLE group was higher than cotrol group (p<0.01). With appropriate rTMS treatment, the expression of c-Fos of TLE rats was significantly decreased (P <0.01) but was still significantly higher than control group (p<0.01). The expression of NGF of TLE group was significantly lower than control group (P <0.01). With appropriate rTMS treatment, NGF expression of TLE group was significantly increased (P <0.01). Conclusion Appropriate rTMS can reduce the c-FOS expression while increase the NGF expression in the hippocampus of TLE rats, which plays a neuroprotective role in improving the pathological damage of epilepsy. Related Articles | Metrics

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The Role of JNK in the Mechanism of Palmitate-induced Insulin Resistance in Skeletal Muscle Cells Jing GAO 2,2 2011, 39 (1):  53-55 .  Abstract ( 1485 )   PDF (2268KB) ( 2560 )   Objective: To study the role of c-Jun N-terminal kinase (JNK) in the mechanism of palmitate-induced insulin resistance in L6GLUT4myc skeletal muscle cells. Methods: In the first part of this study, L6GLUT4myc muscle cells were divided into two groups and treated with 0.3 mmol / L palmitate or solvent BSA for 16 h, respectively. In the second part of this study, JNK inhibitor was added to the medium during the last 30 min incubation with palmitate or BSA. The amount of GLUT4myc on the cell surface and the phosphorylation of protein kinase B (Akt), JNK and insulin receptor substrate1 (IRS1) were measured in the absence or presence of insulin respectively. Results: Compared to the solvent group, insulin-stimulated GLUT4myc translocation and phosphorylation of Akt decreased in palmitate group (P <0.05); phosphorylation of JNK and IRS1Ser307 did not change. Conclusion: Palmitate causes insulin resistance in L6 muscle cells, and its mechanism may not involve JNK, or JNK plays a minor role. Related Articles | Metrics

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Study of Inhibition Effect of LBP on Blood Vessel Endothelium Function Damnification induced by DNTP 2011, 39 (1):  56-58 .  Abstract ( 1386 )   PDF (2184KB) ( 2332 )   Objective: To explore the mechanism of inhibition effect of lycium barbarum polysaccharide (LBP) on blood vessel endothelium function damnification induced by DNTP. Method: The ex vivo aorta pectoralis vascular ring (EVAPVR) of rats and cultured human umbilical vein endothelial cells (HUVEC) were incubated with DNTP for 30 min, LBP was used as protection and superoxide dismutase (SOD) was used as control. The endothelial-dependent relaxation reaction (EDRR), endothelial cell monolayer permeability (ECMP) and biochemical index were detected. Results: Vascular circle experimental result showed that LBP (10mg/mL) obviously reduced the inhibition of ACh-induced EDRR induced by DNTP(4.5μmol/L); Cell experimental result showed that LBP (10mg/mL) obviously reduced the ECMP and MDA, timproved the SOD and nitric oxide(NO) (P＜0.05, respectively), the effect was corresponded on the SOD control(P＞0.05, respectively).Conclusion: LBP inhibited the blood vessel endothelium function damnifivation induced by DNHP,which may be related to the oxidative stress. Related Articles | Metrics

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Optimization of the method to culture human small cell lung cancer cell line H446 tumor cell spheres 2011, 39 (1):  59-61 .  Abstract ( 2503 )   PDF (2216KB) ( 2653 )   Abstract Objective: To optimize the method to culture the tumor cell spheres in small cell lung cancer (SCLC) cell line H446 in vitro. Methods: Using the serum- free culture technology, we observed the growth situation of the H446 tumor cell spheres in the following culture conditions. The cells were inoculated to ultra low adherent plates and non-ultra low adherent plates. The cell density was adjusted to 1 ×104/ mL, 1×105/ mL, 1×106/ mL and 1×107/ mL. The cell spheres were blowed into single cells through micropipettor blow method and pinhead blow method in order to further passages. Results: （1）In ultra low adherent plates, the tumor spheres appeared clearly the next day, while in the tenth day in non-ultra low adherent plates. （2）At the concentration of 1×106/ mL, there were about seven spheres per high power field and dozens of cells even hundreds of cells for each sphere.（3）In the pinhead blow method, the tumor spheres in second generation showed better activity and proliferative ability. Conclusion: Chosing ultra low adherent plate, the inoculum density of 1×106/ mL and the pinhead blow method can separate tumor spheres effectively. Related Articles | Metrics

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Study on Preventive Effects of Pregnancy-induced Hypertension of XSZⅡ in RatStudy on Preventive Effects of Pregnancy-induced Hypertension of XSZⅡ in RatStudy on Preventive Effects of Pregnancy-induced Hypertension of XSZⅡ in RatStudy on Preventive Effects of Pregnancy-induced Hypertension of XSZⅡ in Rat 2011, 39 (1):  62-64 .  Abstract ( 1060 )   PDF (2228KB) ( 2568 )   Related Articles | Metrics

短篇论著

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Analysis of 182 cases with labor analgesia 2011, 39 (1):  67-68 .  Abstract ( 1540 )   PDF (1478KB) ( 2438 )   Analysis of 182 cases with labor analgesia 【Abstract】：Objective: To compare the analgesic effects and safety of epidural ropivacaine 、bupivacaine or levobupivacaine combined with fentanyl in labor analgesia. Methods: A total of 182 women of vaginal delivery were randomly devided into three groups: ropivacaine group (n=72, group A) 、bupivacaine group(n=53, group B) and levobupivacaine group(n=57, group C). vital signs , Analgesic efficiency，sensory levels, and motor block (Bromage score) were recorded degree of motor block(modified Bromage score) , fetal heart rate , neonatal Apgar score for 1 minute and 5minutes, duration of laboure and mode of delivery in the three groups were recorded and compared. Results: Good analgesic effects were observed in all the three groups, the vital signs in the three groups were stable, the second stage of delivery in group B was significantly shorter and MBS scores were significantly higher than those of group A and group C, there were no significant difference between groups of duration of laboure and mode of delivery、neonatal Apgar score. Conclusion: The epidural ropivacaine、bupivacaine or levobupivacaine combined with fentanyl showed good analgesic effects and safety in labor. Bupivacaine could shorten the second stage but showed greater motor block compared to ropivacaine and levobupivacaine . Related Articles | Metrics

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Application of Proseal Laryngeal Mask Airway and Laryngeal Tube Suction in Laparoscopic Cholecystectomy 2011, 39 (1):  69-70 .  Abstract ( 1605 )   PDF (1509KB) ( 2240 )   Abstract Objective: To compare the effect of the proseal laryngeal mask airway (PLMA)and laryngeal tube suction (LTS) for ventilation in laparoscopic cholecystectomy. Methods: Forty patients undergoing elective laparoscopic cholecystectomy were enrolled in this study. The patients were randomly divided into two groups, PLMA group and LTS group,with 20 cases in each goup. The mean blood pressure (MBP), heart rate (HR), and oxygen saturation of pulse (SpO2) were recorded during operation. pH, PaCO2, Paw were recorded at before pneumoperitoneum , 15 min after pneuoperitoneum setting and 15min after pneuoperitoneum deflation. Results: Compared with before pneumoperitoneum, the PaCO2, Paw of 15 min after pneuoperitoneum setting were significantly increased（P<0.05）. The data of the three time points between two groups had no significantly difference（P＞0.05）. Conclusion: Applicated the PLMA and the LTS in laparoscopic operation, could ensure the stability of ventilation and hemodynamics, was the optimal selection. Related Articles | Metrics

临床论丛

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18F-FDG PET/CT affects restage and management of patients with colorectal cancer post-operation 2011, 39 (1):  72-73 .  Abstract ( 1604 )   PDF (1401KB) ( 2211 )   Objective: To evaluate the impact of 18F-FDG PET/CT in restage and management of patients with colorectal cancer post-operation. Methods: 36 patients underwent whole-body 18F-FDG PET/CT. The diagnosis standards consisted of histopathology or follow-up by conventional imaging methods. Analyzing and comparing the restage and management before and after PET/CT. Results: PET/CT yielded a correct diagnosis in 34 out of 36 patients, and altered therapy management in 17 patients: 2 patients had therapy alteration within the management, 15 patients had therapy alteration among the surgery resection, the medical treatment (chemotherapy and radiotherapy) and follow-up. The change rate was 47.2 %( 17/36) Conclusion: 18F-FDG PET/CT improves restage and management of patients with colorectal cancer post-operation. Related Articles | Metrics

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The dynamic changes of matrix metalloproteinase-9 and lipoprotein associated-phospholipase A2 in sera of patients with acute brain infarction The dynamic changes of matrix metalloproteinase-9 and lipoprotein associated-phospholipase A2 in sera of patients with acute brain infarction 2011, 39 (1):  76-78 .  Abstract ( 1466 )   PDF (2914KB) ( 2182 )   Abstract Objective：To observe the clinical usage of matrix metalloproteinase-9 and lipoprotein associated-phospholipaseA2 in acute brain infarction.Methods：146 acute brain infarction patients,50 non-infarction patients,and 50 heathy persons were observed. The 2 indexes in sera were examed and analysed. The dynamic changes on the day of administration and 5、10、15、20 days after treatment were compared.Conclusion：The 2 indexes and their dynamic changes can help the diagnosis and the evaluation of severity and infarction area. Related Articles | Metrics

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Changes of biochemical markers of bone turnover and mineral density in fracture patients with type 2 diabetes mellitus 2011, 39 (1):  78-79 .  Abstract ( 1410 )   PDF (1516KB) ( 2159 )   ABSTRACT: Objective By comparison of the changes of sBAP, sCTX, uCTX bone specific biochemical markers and BMD in 52 bone fracture patients and 89 non-fracture patient with type 2 diabetes to explore the mechanism of bone fracture and biochemical markers for prediction of fracture value in type 2 diabetes patients. Methods Dual-energy X-ray absorption metry was used to measure BMD of lumbar spine (L2-4) and the trochanter (Troch), intertrochanteric (Ward's), femoral neck (Neck) of left hip bones ; ELASA enzyme-linked immunosorbent assay shares biochemical markers sBAP, sCTX, uCTX. Results Age, BMI, HbAc1, BMD have no significant difference between fractures and non-fracture patients with type 2 diabetes mellitus； sBAP(31.6±8.0 vs 38.8±6.3) of the bone specific biochemical markers was lower in fracture group than non-fracture group ,howere, sCTX( 9.8±2.5 vs 8.6±1.7), uCTX (35.2±8.2 vs 32.4±7.6 )as the sign of bone resorption have no differentiation between the two groups Conclusion :In fracture patients of type 2 diabetes, the activity of osteoblasts decrease, but osteoclast activity and bone mineral density don’t significantly change. The bone turnover ratio decrease in fracture patients than non-fracture patients of type 2 diabetes. Bone biochemical markers possibly be the early indicators of fracture of type 2 diabetes. Related Articles | Metrics

临床论丛

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The Diagnosis and Treatment of caecal carcinoma Presenting Appendicitis 2011, 39 (1):  80-81 .  Abstract ( 1173 )   PDF (1453KB) ( 2207 )   Abstract [Objective]: To summarize the clinical features of caecal carcinoma complicated with appendicitis, investigate the method of early diagnosis and treatment for caecal carcinoma. [Methods]: There were 33 patients with caecal carcinoma between May 2004 and December 2009 and 6 of them presenting appendicitis. The clinical data were analyzed. [Results]: 2 cases diagnosed in exploration; 3 cases diagnosed by preoperative examination, but the average delay of diagnosis compared with the first visit is 3.6 months; 1 case was misdiagnosed. [Conclusion]: There are some imperative measures to decrease the misdiagnosis by deepening recognization of caecal carcinoma presenting appendicitis, giving more effective preoperative examination, exploration, intraoperative frozen pathology and postoperative routine follow-up. Related Articles | Metrics

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Endoscopic transnasal approach and neuronavigation for surgical treatment of skull base chordomas 2011, 39 (1):  86-87 .  Abstract ( 1549 )   PDF (1464KB) ( 2166 )   [Abstract]　Objective: To discuss the surgical method and clinical effect of endoscopic transnasal approach and neuronavigation to resect chordomas of skull base. Methods: Twenty-five cases of chordomas operated via transsphenoidal sinus approach were retrospectively analyzed. Results: Tumor was totally removed in 9 patients, subtotally in 13, and partially in 3. Clinical symptoms of 20 patients were relieved after operation, and not relief in 5. There was no operative death or any postoperative complications. During the follow-up periods, tumor recurred in about 12 months after operation in 1 cases only got partial resection of the tumor, and recurred 3 years after operation which metastasized to the distal organs in 1. Conclusion: Endoscopic transnasal approach and neuronavigation is adapted to resect chordomas of saddle area or middle and upper clivus and those lesions involving nasal cavity, nasal sinuses and nasopharynx. Endoscopic transnasal approach and neuronavigation has many advantages, such as safety, efficiency, minimal invasion, simple manipulation, timesaving, good results. Related Articles | Metrics