Table of Content

15 April 2014, Volume 42 Issue 4 Previous Issue    Next Issue
---
细胞与分子生物学
实验研究
临床研究
流行病学调查
应用研究
综述


For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

细胞与分子生物学

Select

The Short Term Effect of Insulin on Proinsulin Gene Expression of HIT-T15 Insulinnoma Cells <FONT style= 2014, 42 (4):  289-292 .  Abstract ( 478 )   PDF (380KB) ( 2583 )   [Abstract] Objective To investigate the short term effect of insulin on proinsulin gene expression of HIT-T15insu? linnoma cells（pancreatic isletβ-cell）. Methods The HIT-T15cells were randomly divided into four groups.Blank Con? trol Group (LG) : complete medium contain 1.4mmol/L glucose. Control group (LGC)：co-cultured nifedipine with medium in order to restain endogenous insulin release. Experimental group (LINS or HINS) add0.5U/L insulin or5U/L insulin on top of LGC. After being stimulated for0, 30, 60, 90, 120mins, proinsulin (PI) mRNA level were assessed by semi-quantitative RT-PCR. Insulin receptor substrate1(IRS1) tyrosine phosphorylation was detected by immunocytochemistry. Results (1) Expression of PI was up regulated by both LINS and HINS, and peak at60mins. (2) After stimulation for 30mins, the level of IRS1tyrosine phosphorylation in the experimental group was significantly higher than control group, and the peak time be? tween LINS and HINS was different. (3) Between group of LG and LGC, the expression of PI mRNA and IRS1tyrosine phos? phorylation show no difference.Conclusion Short term exogenous insulin stimulation can promote expression of proinsulin genes，which is concentration dependent. The expression and regulation of PI were related with IRS1signal transduction. Related Articles | Metrics

Select

Study of Mechanism of Proinsulin Gene Mutations in Diabetes Mellitus 2014, 42 (4):  293-296 .  Abstract ( 606 )   PDF (971KB) ( 2668 )   　　　Abstract Objective: To construct several human proinsulin mutants related to diabetes and express in INS-1 cell. Methods: Human mild proinsulin gene as template ,use site-directed mutagenesis，generate four human proinsulin mutants by PCR，sequence each mutant，transfect four mutants、empty plasmid and mild plasmid into INS-1 cell by liposome 2000，determine insulin values in each cell solution by radioimmunoassay. Results: Proinsulin mutants are confirmed by sequence determination ,insulin values in culture solution of H-C(B19)G、 H-L(B11)P、H-R(S6)C mutants are less than that of wild type and found no significant differences statistically with empty plasmid. insulin values of H-F(B25)L is more than that of empty plasmid and the former three mutants. Conclusions: Successfully construct and express four human proinsulin mutants, different mutants result in diabetes through different mechanism. Related Articles | Metrics

Select

The Effect of Silencing Mcl-1by siRNA on Biological Behavior of Gastric Cancer MGC-803Cell 2014, 42 (4):  297-300 .  Abstract ( 566 )   PDF (443KB) ( 2593 )   Objective To investigate the effect of inhibiting Mcl-1 gene expression on the biological behavior of gastric cancer cell MGC-803 by using a small interference RNA(siRNA)strategy. Methods Synthesized siRNA targeting Mcl-1(Mcl-1siRNA group) was transfected into MGC-803 cells,additionally,MGC-803 cells transfected with negative siRNA(Mcl-1siRNA-NC group),MGC-803 cells transfected with Lipofectamine 2000（liposomes control group）and vacant MGC-803 cells（blank control group） were used as controls.MTT was adapted to investigate the proliferation of MGC-803 cells after transfection of Mcl-1 siRNA. After 48h transfection of Mcl-1 siRNA, the flow cytometry(FCM) was used to examine the apoptosis cells in the four groups. Cell cycle was detected by FCM in different groups. Polycarbonate membrane transwell chamber was used for detecting the abilities of invasion and migration of the cell line. Results The absorption value of MGC-803 cells decreased greatly after transfected with Mcl-1siRNA for 24、48 and 72 h compared to those in control groups(P<0.05); after transfected 48h, apoptosis rate in Mcl-1siRNA group was higher than in the blank control group, liposomes control group and Mcl-1siRNA-NC group(%:19.61±1.66 vs 3.69±0.37 vs 3.54±0.47 vs 3.68±0.55,F=12.230,P＜0.05),G0/G1 [(41.03±1.86)%] and G2 / M phase ratio [(1.80± 0.46)%] in Mcl-1 siRNA group were lower than in the three control groups, S phase ratio [(57.17±1.72)%] was higher than in three control groups(P<0.05). Transmembrane cell number of Mcl-1 siRNA group in polycarbonate membrane transwell chamber(42.00±4.00,76.33±3.51 respectively)were less than of the blank control group(79.33±3.51,108.00±3.61 respectively),liposome control group(74.67±2.52,110.67±4.04 respectively)and negative control group (77.33±3.06,109.33±4.51 respectively). However,there was no significant difference in above index among the control groups. Conclusion Inhibition the expression of Mcl-1 can effectively supress the growth, invasion and migration, and can promote apoptosis in gastric cancer cells. Related Articles | Metrics

Select

The Diagnostic Value of Sox2mRNA Transcription Level in Bronchoscopy Biopsy Specimens in Lung Cancer 2014, 42 (4):  301-304 .  Abstract ( 597 )   PDF (671KB) ( 2739 )   [Abstract] Objective To study the diagnostic value of Sox2 mRNA transcription level in bronchoscopic biopsy specimens from lung cancer patients. Methods The expression of Sox2 mRNA was detected using RT-PCR from 100 hu? man lung cancer biopsy and 18 non-cancer lung biopsy through bronchoscopy. The expression of Sox2 protein was examined by immunohistochemistry from 50 cases of lung cancer biopsy, 32 cases of benign lung lesions and 18 cases of pericarcino? matous normal lung tissues. Then the relationships between Sox2 mRNA transcription level and lung cancer clinical patho? logical parameters were analyzed to test the diagnostic value of Sox2 transcription level. Results The transcription of Sox2 mRNA and its protein expression level were significantly higher in lung cancer than that in benign pulmonary disease tissues （P＜0.05） . The transcription of Sox2 mRNA was not correlated with age, gender, histology, lymph node metastasis, TNM stage and differentiation of lung cancer patients (P＞0.05). The Sox2 mRNA yielded an area of 0.748 under the ROC curve with the sensitivity of 85.0% and the specificity of 61.1%, taking the cut-off value of 0.513. Conclusion The Sox2 mRNA might be a useful diagnostic marker for lung cancer. Related Articles | Metrics

Select

Expression Level of Sphingosine Kinase1and Nuclear Factor-κB p65in Non-Small Cell Lung Cancer and Their Relationship with Tumor Prognosis 2014, 42 (4):  305-308 .  Abstract ( 489 )   PDF (714KB) ( 2724 )   [Abstract] Objective To investigate expression level of sphingosine kinase1(SPHK1) and nuclear factor-κB (NF- κB) in non-small cell lung cancer (NSCLC) and their relationships with invasion, metastasis and prognosis of NSCLC.Meth? ods Ninety-three NSCLC specimens and paraneoplastic normal lung tissue from conventional surgery were confirmed by histology. Expression of SPHK1and NF-κB were detected by Immunohistochemistry on paraffin sections. Primary antibody were Rabbit Anti-Human SPHK1and Rabbit Anti-Human NF-κB p65, which were incubated 1hour in water bath. The secondary antibody was HRP-Polymer anti Mouse IgG, which was incubated20minutes in water bath.Results SPHK1ex? pression was positive in96.8% (90/93) of NSCLC specimen which is higher than in paraneoplastic normal lung tissue in which the positive rate is18.3% (17/93) ; NF-κB expression was positive in89.2% (83/93) NSCLC which is higher than the in paraneoplastic normal lung tissue in which the positive rate is 12.9% (12/93). The expression of SPHK1and NF-κB in NSCLC was positively correlated (r=0.464, P<0.01). TThe expression levels of SPHK1and NF-κB p65in NSCLC patients with were positively related to TNM staging and lymph node metastasis. SPHK1expression and NF-κB p65expression lev? el were higher in the deads than in survivals. There was no statistical significance in different expression intensity of SPHK1 and NF-κB p65in patients with NSCLC who had differences in gender, age, tumor size, tumor location, histological type. Survival analysis showed that survival time of patients of NSCLC with high expression of SPHK1was shorter than those in the group with low SPHK1expression, and the difference was statistically significant (χ 2 =14.025, P<0.01). Conclusion In the process of NSCLC invasion and metastasis，SPHK1may play an important role through NF-κB, and it can predict prognosis of NSCLC patient. Moreover, it will become a potential target for NSCLC target. Related Articles | Metrics

Select

Silencing of MTA1Attenuates Nasopharyngeal Carcinoma Metastasis in vitro TU ying hua 2014, 42 (4):  309-311 .  Abstract ( 522 )   PDF (312KB) ( 2583 )   [Abstract] Objective To investigate the effects of MTA1knock down on migration and invasion of NPC cell5-8F in vitro. Methods RNAi (Si-MTA1-01and Si-MTA1-02) that can transiently silenced MTA1was designed, synthesized and transfected into5-8F cells by lipofectamine2000. Control group (transfection with nonsense sequence) was also estab? lished. The efficiency of MTA1depletion was determined by q-PCR and Western blot. Wound-healing assay ,Matrigel inva? sion assay and thesolid-phase adhesion assay were performed to investigate the effect of MTA1knockdown on5-8F cell me? tastasis. Results Transiently knock down of MTA1decreased MTA1transcription and expression in 5-8F cells compared to shRNA-con cells, showing by Real-time PCR and western blot. The invasion and migration of the cells transfected with siRNA-MTA1were much weaker than the control group (P<0.05). Conclusion silencing MTA1gene can effectively in? hibit the migration and invasion of nasopharyngeal carcinoma cell, and might be a promising target for NPC treatment. Related Articles | Metrics

Select

The Changes of Circulating Th17and Tc17Cells in Patients with Colorectal Polyp,Adenoma and Cancer 2014, 42 (4):  312-314 .  Abstract ( 535 )   PDF (275KB) ( 2728 )   [Abstract] Objective To compare ratio of circulating Th17over Tc17cells in patients with colorectal polyp (CRP), colorectal adenoma (CRA) and colorectal cancer (CRC)，and analyse their relationship.Methods The ratio of circulating Th17over Tc17cells in93patients（17CRP,34CRA and42CRC）and17healthy donors were examined by flow cytometry; Serum levels of IL-17A and IL-23were tested by ELISA.Results The ratio of circulating Th17over Tc17cells and the se? rum levels of IL-17A and IL-23were the lowest in healthy control groups, medium in CRP and CRA groups and the highest in CRC groups (P<0.05). The proportions of Tc17cells in CRA groups were significantly higher compared to those in CRP groups，but no statistical significance of proportion of circulating Th17cells as well as serum levels of IL-17A and IL-23be? tween CRA and CRP groups was observed (P>0.05). Further analysis showed that there was a positive correlation between proportions of circulating Th17and Tc17cells (rs=0.594, P<0.001). Conclusion The proportions of circulating Th17and Tc17cells were positively related and the ratio of Th17over Tc17was markedly increased from CRP group or CRA group to CRC group. Related Articles | Metrics

Select

Roles of Pericyte in the Angiogenesis of Endometriosis ZHAO cai yun 2014, 42 (4):  315-317 .  Abstract ( 651 )   PDF (270KB) ( 2598 )   Abstract: Objective To study the expression of pericyte and make further discussion about the association between pericyte and angiogenesis in the endometriosis. Methods the main group consisted of 20 ectopic endometrium 、20 eutopic endometrium during proliferation phase,20 eutopic endometrium during secretory phase of patient with endometriosis .The control group presented 20 normal endometrium during proliferation phase and 20 normal endometrium during proliferation phase of women without entometriosis.The histological morphology ,mean density of microvessel(MVD),pericytes number（PN） as well as the average ratio of pericyte to endothelial cells in microvessels were measured by immunohistochemical and morphological assesses .Results The expression of MVD was increased from the ectopic endometrum tissue ,eutopic endometrum to normal endometrum,while the expression of PN and PN/MVD was opposite.we also found that the PN in eutopic endometrum was slightly lower than it in normal endometrium ,no mater in secretory stage or proliferativa stage,there was no statistical difference between them.But The difference of the PN/MVD had significant meaning (P<0.01). Conclusion Pericytes play an important role in the angiogenesis of the endometriosis ,pericyte coverage contributing to a mature functional vasculature of endometriosis. Related Articles | Metrics

实验研究

Select

Establishment of Modified Brain Death Model in Pig 2014, 42 (4):  318-320 .  Abstract ( 447 )   PDF (311KB) ( 2571 )   Objective To establish a stable and reliable brain death model in swine, and to provide a more physiological way to investigate brain function, transplantation immunology during brain death. Methods On the basis of classic method of increasing epidural intracranial pressure, Codman intracranial pressure monitoring probes were implanted in landrace pigs invasively, the brain death models were established under real-time monitoring of the intracranial pressure. Results 12 experimental landrace pigs, one died of anesthetic accident, 11 were successfully induced model of brain death, the success rate was 100%. With effective respiratory and circulatory support the brain death models can maintain 31.3 ± 4.7h. Transcranial Doppler, EEG, ECG, mean arterial blood pressure and other monitoring shows that brain death model-making process is stable and reliable. Conclusion This method of inducing brain death model (under ICP monitoring) is stable and easy to be standardized. Related Articles | Metrics

Select

Hypoglycemic Mechanism of Total Saponins of Momordica Charantia in Type2Diabetes Mellitus Rats 2014, 42 (4):  321-324 .  Abstract ( 500 )   PDF (1789KB) ( 2907 )   Objective To observe the hypoglycemic mechanism of total saponins of Momordica charantia(MC) in type 2 diabetes rats. Method: 8 rats were randomly selected as normal control group in 60 SPF SD rats. Other rats were used to build the type 2 diabetes models. The type 2 diabetes rats models were established by eating food containing the higher lipid and sugar and by intravenous injection of a low dose of streptozotocin. After the disease models were successfully induced, the model rats were then randomized into five groups: the model control group (n=8), the metformin group (n=8) and the three dose total saponins of MC group (n=24). The total saponins of MC group was administrated with total saponins of MC 100, 200, 400mg/(kg?d) for 8 weeks, the metformin group was administrated with metformin 50mg/(kg?d) for 8 weeks. At the end of the experiment, the fasting blood glucose and insulin were measured. At the same time, a part of pancreas islet, liver and skeletal muscle were preserved. The pancreas islet structure, the hepatic glycogen and Glucose transporter 4(GLUT4) expression were observed by electron microscope, glycogen dyeing and western blot methods respectively. Result:After 8 weeks treatment, compared to type 2 diabetes model group, fasting blood glucose and insulin values in MC group reduced more obviously, however, the amount of skeletal muscle GLUT4 expression, insulin granules and hepatic glycogen increased obviously in MC group. Conclusion: Total saponins of MC has the hypoglycemic activity. It’s mechanisms maybe include promoting the hepatic glycogen synthesis, inhibiting the hepatic glycogen decomposition and promoting insulin sensitivity by increasing peripheral tissue’s GLUT4 expression. Related Articles | Metrics

Select

Detection of Platelets and Endothelial Cell-Derived Microvesicles in Rat Peripheral Blood 2014, 42 (4):  325-328 .  Abstract ( 523 )   PDF (363KB) ( 2787 )   [Abstract] Objective To establish a flow-cytometric method for detection of microvesicles (MVs) in rat peripheral blood, detect platelet-derived MVs (PMVs) and endothelial cell-derived MVs (EMVs) in blood from ischemic preconditioning (IPC) treated rats. Methods Blood was withdrawn from rat abdominal aorta and anticoagulated with sodium citrate. Platelet-free plasma (PFP) was obtained after two centrifugations at room temperature. PFP was incubated with FITC-conjugated mouse anti-rat CD61 or PE-conjugated mouse anti-rat CD144. Standard beads in diameter 1 and 2 μm were used for size calibration and absolute counting, respectively. Analysis was performed on flow cytometer. Results When 3.5% sodium citrate was blent with blood according to the volume ratio of 1:4, clear supernatant was obtained after centrifugation. Signals of particles smaller than 1 μm accounted for more than 99% of all. PMVs and EMVs were CD61 positive and CD144 positive, respectively. Their diameters were smaller than 1 μm. The concentration of PMVs and EMVs in peripheral blood from IPC treated rats was 4053±1987/μL and 4870±825/μL, respectively. Conclusion The method for MVs detection by flow cytometry was successfully established and optimized, and verified through detecting PMVs and EMVs in peripheral blood from IPC treated rats. Related Articles | Metrics

Select

Effects and Mechanism of Alcea Rosea Roots in Ethylene Glycol Induced Nephrolithiasis in Rats Jia-Xi YAO 2014, 42 (4):  329-332 .  Abstract ( 495 )   PDF (313KB) ( 2816 )   Objective To study the beneficial effect and mechanism of Alcea rosea roots in ethylene glycol induced nephrolithiasis in rats. Methods We randomly divided 60 male Wistar rats into six groups, control group, model group, Alcea rosea roots lower-dose preventive group, Alcea rosea roots high-dose preventive group, Alcea rosea roots lower-dose curative group, Alcea rosea roots high-dose curative group. Control group was provided free access to food and normal drinking water, model group received ethylene glycol drinking water and was fed with normal chow, preventive group as model group received Alcea rosea roots in low and high dose, curative group as model group received Alcea rosea roots in low and high dose from day 15 to day 28. At the end of the experiment, various renal functional and injury markers such as urine volume, calcium, phosphate, magnesium, urea, creatinine, and oxalate were evaluated using urine, serum, and kidney homogenate. The kidneys were removed and prepared for histological evaluation of calcium oxalate deposits. Results The model groups increased the urine output, urea, creatinine, 24h urine Ca2+, and oxalate and MDA compared to the control group（P<0.05）. The preventive and curative groups increased the GSH and SOD compared to the model group（P<0.05）. The preventive and curative groups reduced the urea, creatinine, 24h urine Ca2+, urine oxalate, MDA compared to the model group（P<0.05）. It was also reduced the number and size of calcium oxalate crystal deposits and protected the kidneys from serious damage compared to the model group. Conclusion The extract of Alcea rosea roots can prevention and treatment calcium oxalate urinary stone formation in rats and protect renal function. Related Articles | Metrics

Select

GATA4Expression During Induction of the Cardiomyocyte-Like Cells Differentiation Regulated by miR-122 2014, 42 (4):  333-336 .  Abstract ( 488 )   PDF (446KB) ( 2813 )   [Abstract] Objective To identify the miR-122which regulateing GATA4expression during the induction of rat bone marrow mesenchymal stem cells (bMSCs) differentiating into cardiomyocyte-like cells.Methods BMSCswere isolat? ed from bone marrow and induced to differentiate into cardiomyocyte-like cells using5-azacytidine. The miR-122which may regulate expression of GATA4were predicted using miRanda and TargetScan softwares and identified by dual luciferase report system. The expressions of miR-122and GATA4were determined using q-PCR during the differentiation of bMSCs into cardiomyocyte-like cells. Results The induced cells were completely in contacted with adjoining cells and uniform in shape and aligned parallelly. Cardiac troponin I (cTnI) expression was detected by immunofluorescence cytochemistry. Using dual luciferase reporter systemin vitro, miR-122were proved to be able to effectively inhibit GATA4expression by binding the 3′ UTR of GATA4mRNA. q-PCR results showed that the expression of miR-122is negatively correlated with that of GATA4mRNA transcription.Conclusion These results indicated that miR-122regulate the expression of GATA4during the induction of cardiomyocyte-like cells. Related Articles | Metrics

Select

The Effect of Erhuang Decoction on Transforming Growth Factor-β1of Lung Tissues and the Expression of Leukotriene-33in Asthmatic Rats yanhui NIE 2014, 42 (4):  337-340 .  Abstract ( 506 )   PDF (810KB) ( 2598 )   [Abstract] Objective To investigate the effect of Erhuang decoction on TGF-β1expression of lung tiusses and the concentrations of IL-33in asthmatic rats. Methods Fifty Sprague- Dawley rats were randomly divided into five groups equally: Control group, Asthmatic group, Budesonide aerosol group, High-dose Erhuang decoction group (68g/kg)and Low dose Erhuang decoction group(17g/kg). The model of asthma was established by ovalbumin (OVA) sensitizing and challeng? ing. Then Erhuang decoction and budesonide aerosol was used respectively for intervention therapy. Histologic HE staining were used to observe the general pathologic alteration and to analyze the total bronchial wall area (Wat) and the muscle wall area(Wam). The protein expressions of TGF-β1 in the lung tissues were detected by immunohistochemistry. The concentra? tions serum IL-33 and BALF were tested by sandwich ELISA. Results There was significant reduction in the infiltrated inflammatory cells in all drug intervention groups compared with asthma group; The Wat and Wam in asthmatic group was significantly higher in than those in Budesonide aerosol group，High-dose Erhuang decoction group and Low-dose Erhuang decoction group ( Watμm 2 /μm :54.99±8.82, 52.28±7.61, 58.53±7.63vs79.50±5.64, P＜0.05; Wam μm 2 /μm :22.74±2.73, 20.63±1.72, 21.20±4.50vs 30.16±1.68, P＜0.05); Compared with control group, BALF and serum IL-33concentration were significantly higher in asthmatic group. Compared with asthmatic group, all the indicators were significantly decrease in the treatment groups after drug intervention (P＜0.05). Andthere was no significant difference between the treatment groups in all the indicators. TGF-β1expression in lung tissues in asthmatic group were significantly higher than that in control group (12.60±2.25vs1.67±0.17). Compared with asthmatic group, there was significantly reduction of TGF-β1 expression in the Budesonide aerosol group (5.51±2.48), High-dose Erhuang decoction group (5.22±2.52) and Low-dose Erhuang decoction group (6.92±2.18) (P＜0.05). There were no significant difference between the treatment groups. TGF-β1 expression and se? rum IL-33concentration in asthmatic rats were positively correlated with Wat and Wam.Conclusion The effects of Er? huang decotion on ameliorating the progression of airway remodeling about asthmatic rats may be partially by regulating TGF-β1and IL-33. Related Articles | Metrics

临床研究

Select

The Significance of Thin-Section CT in Quality Assurance and Control of Non-Small Cell Lung Cancer Therapy through 2014, 42 (4):  341-344 .  Abstract ( 541 )   PDF (547KB) ( 2652 )   [Abstract] Objective:To explore the clinical value of the thin-section CT scanning in all links of 125I seed implantation in treating non-small cell lung cancer.Methods:137 patients comfired NSCLC,the preoperative target was delineated by CT scanning,and the implantation plan was accomplished according to the TPS; Intraoperative 125I seeds were implanted under guidance of CT, and then seeds were scanned by CT layer-by-layer to validate and correct position in real time ;Postimplantation dosimetry was validated with help of CT scanning.If necessary, seed distribution and seed number were adjusted to make dose distribution conform with the principle of available and micro-invasive; Following up and evaluating termly also needed to be accomplished by CT scanning. Results: The DVHs showed the dose line in targets could meet the requirement of prescription dose, the surrounding organs at risk were within the scope of its tolerance dose.129 patients in all 137 patients implantation seeds were the same to seeds of TPS planning,the coincidence rate was 94%.CT scanning showed the local control efficient rate was91.9% after 6 months.The 1-year and 2-year survival rates were 91.2% and 50.4% respectively.During treatment none of them had serious operation-related complication. Conclusion:The thin-section CT was applied in all key links of 125I seed implantation in treating non-small cell lung cancer, especially the clinical value was significant and irreplaceable in quality assurance and quality control of seed implantation treatment. Related Articles | Metrics

Select

Relationship among Brachial-Ankle Pulse Wave Velocity, Early Renal Dysfunction and Left Ventricular Hypertrophy in Patients with Hypertension LI pu 2014, 42 (4):  345-348 .  Abstract ( 439 )   PDF (298KB) ( 2754 )   [Abstract] Objective To study the relationships among brachial-ankle pulse wave velocity (baPWV) , early renal function and left ventricular hypertrophy (LVH) in patients with essential hypertension. Methods Four hundred and twen? ty-seven patients aged60-90years were selected from the patients with essential hypertension. The patients were divided in? to four groups by quartile of the baPWV. Biochemical index were recorded. The eGFR were calculated using Cockcroft Gault formulation. Ultrasonic cardiogram(UCG) were employed to calculate the left ventriclar mass index (LVMI).Results There were significant differences in the age, ankle brachial index(ABI), systolic blood pressure(SBP), mean arterial pressure (MAP),pulse pressure(PP), fasting blood glucose(FBG), LVMI and eGFR among the four groups. Given the age and FBG , baPWV was positively correlated with SBP (r=0.295, P＜0.01), MAP(r=0.187, P＜0.01), PP(r=0.292, P＜0.01), LVMI(r= 0.159,P＜0.01), but negatively related to eGFR (r=-0.314, P＜0.01). Multiple linear regression analysis indicated that pressure(DBP) still were still independent risk factors of eGFR, after adjusting for other cardiovascular risk factors, age, baP? WV and diastolic blood (t=8.251，6.834，2.142，P＜0.05). Systolic blood pressure(SBP) and ankle brachial index(ABI) were independent risk factors of LVMI ( t=4.557,3.016，P＜0.05), but the baPWV was not an assured independent risk factor of LVMI(t=1.722, P=0.086). Conclusion baPWV played an important role in evaluating the cardiovascular risk factors and early renal dysfunction caused by hypertension. Related Articles | Metrics

Select

Reperfusion Therapy and White Blood Cell Count in Patients with Acute Myocardial Infarction 2014, 42 (4):  349-351 .  Abstract ( 509 )   PDF (296KB) ( 2578 )   [Abstract] Objective To study the relationship between blood cell count and cardiac events in acute ST elevation myocardial infarction patients treated with reperfusion in the early stage.Methods In this study, we assigned151patients to whom reperfusion therapy had been delivered within 3hours of STEMIsymptominto two groups: primary percutaneous cor? onary intervention group and thrombolysis therapy group. Differences of cardiac events and white blood cell count in these two groups were analyzed in the first 4days. Results In the second day and the forth day, cardiac events rate and white blood cell count were significantly lower in the primary percutaneous coronary intervention group than those in the thromboly? sis therapy group (P＜0.05). In the second day, white blood cell count has a positive correlation with cardiac events rate (r= 0.226,P＜0.05). Conclusion Primary percutaneous coronary intervention decreased blood cell count and cardiac events rate. In patients with acute myocardial infarction, increasing white blood cell was related to cardiac events in the near future. Related Articles | Metrics

Select

Influence of the Altered-Cast Impression Technique on Distal-Extension Alveolar Ridge Morphology and Restorative Result 2014, 42 (4):  352-355 .  Abstract ( 522 )   PDF (538KB) ( 2876 )   [Abstract] Objective To investigate the impact of the modified cast impression technique on distal-extension alve? olar ridge morphology of partially edentulous casts and the restoration outcome of removable partial dentures (PRDs).Meth? ods Fifty-four patients with mandibular free-end dentition defect were selected. Impressions were taken from each patient using both modified cast technique and conventional technique. Coronary cross-sections of the casts were scanned from dis? tal to mesial extension; the images were compared to reveal morphological differences. And the relationship between these differences and location of the cross- sections were analyzed. After the patients were evenly divided into two groups per paired design, the impressions were taken using the respective modified cast and conventional impression techniques to pre? pare RPDs. The subsequent comparisons between the two groups included frequency of adjustments during the adaptive phase, masticatory efficiency of the denture at ending of the adaptivephaseand visible movement of the extension base dur? ing mastication. SPSS 16.0software was used for statistical analysis. Results The number of pixels in the same cross-sec? tion was less in the impression taken with modified cast technique than that with conventional technique. And their differ? ence is of statistical significance (P<0.01). The difference in the number of pixels (D) was negatively correlated with the number of cutting and grinding, and N (location of the cross-sections). In addition, there were statistically significant differ? ences in frequency of adjustments during theadaptivephase(times：1.12±0.77vs3.41±0.82）, masticatory efficiency of the denture (0vs18.5%) and visible movement of the extension base during mastication (96.3%vs74.1%) between the modified cast group and conventional group.Conclusion Morphology of distal-extension alveolar ridge of the casts differs signifi? cantly between the impressions obtained with modified cast technique and the impressions obtained with the conventional technique; the difference increases with greater distance to the distal surface of the terminal abutments. The restoration out? come of RPDs is significantly better with the modified cast impression technique than with the conventional impression tech? nique. Related Articles | Metrics

Select

Rediscovery the Effect of Intensive Insulin Therapy on Stress Hyperglycemia in Critical Trauma Patients 2014, 42 (4):  356-358 .  Abstract ( 473 )   PDF (330KB) ( 2604 )   【Abstract】　Objective　To investigate the effect and safety of intensive insulin therapy on critically trauma patients who had stress hyperglycemia, with different level goal blood glucose in surgery intensive care unit(SICU).Methods We prospectively analyzed SICU patients in 2010.1-2012.7 , whose admission blood glucose and 30 minutes blood glucose were both over 11．1mmol／L ，but without history of diabetes．Of total 153 patients were classified according to their goal blood glucose for three groups：intensive insulin therapy (IIT)group，4.0一6.1mmol/L ；NICE SUGAR(NST)group , 6．2-8．3 mmol／L, cantract insulin therapy （CIT）group was 9.9-11.1mmol/ L; each group had 51 patients. To collecting datas of three groups patients on the，the insulin doses each day, rate of hypoglycemia, final blood glucose,APACHEII scores ,time of the ventilatory support, the days of stay in ICU ,morbidity and mortality rate . Results In three groups patients, time of the ventilatory support, the days of stay in ICU ,morbidity were significantly lower in NST group than CIT group, （P Related Articles | Metrics

Select

Effect of Early Hemofiltration Combined with Traditional Chinese Medicine on Nasal Feeding on Severe Acute Pancreatitis Patients 2014, 42 (4):  359-362 .  Abstract ( 600 )   PDF (320KB) ( 2618 )   [Abstract] Objective To investigate the effects of severe acute pancreatitis (SAP) patients undergoing early hemofiltration combined with traditional Chinese medicine on nasal feeding. Methods 74 patients were divided into traditional Chinese medicine group (group A, 35 cases) and traditional Chinese medicine + hemofiltration group (group B, 39 cases). In traditional Chinese medicine group, patients were given fasting, gastrointestinal decompression, fluid resuscitation, inhibition of pancreatic secretion, antibiotic prophylaxis, parenteral nutrition, traditional Chinese medicine on nasal feeding; In traditional Chinese medicine + hemofiltration group, patients received continuous veno-venous hemofiltration treatment. On admission and first, 3, 7 days post-treatment, the scores of acute physiology and chronic health evaluation (APACHE II), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8) levels were observed. Length of hospital stay, local and systemic complications, surgical intervention, mortality and hospitalization expenses were compared between two groups. Results On admission between the two groups, no statistical significance was seen in the hematocrit, white blood cell count, lactic acid dehydrogenase, blood urea nitrogen, blood glucose, APACHE II score, Ranson’s score and classification of etiology (P>0.05). But APACHE II, TNF-α, IL-6, IL-8 were decreased significantly in group B than in group A after first, 3, 7 days post-treatment (P<0.05). Compared to the traditional Chinese medicine group, the length of hospital stay, local complications, systemic complications, surgical intervention, mortality and hospitalization expense were lower in traditional Chinese medicine + hemofiltration group. Conclusion Traditional Chinese medicine on nasal feeding combined with early hemofiltration could effectively reduce complications, incidence of organ dysfunction and improve the prognosis of SAP patients. Related Articles | Metrics

Select

The Influence of High-Dose Atorvastatin in Heart Rate Variability and Malignant Ventricular Arrhythmia in Patients Underwent Emergency PCI 2014, 42 (4):  363-365 .  Abstract ( 476 )   PDF (299KB) ( 2644 )   Objective To study the influence of high dose atorvastatin (80 mg) on early heart rate variability and malignant ventricular arrhythmia in patients underwent emergency PCI. Methods 350 cases underwent emergency PCI from January 2010 to January 2013 were randomly divided into two groups: high dose atorvastatin group (175 cases) and regular dose atorvastatin group (175 cases); and 85 cases of control were enrolled randomly(control group). The patients of high dose atorvastatin group received atorvastatin 80 mg orally instantly after hospitalization and 40 mg once a day from the second day; The patients of regular dose atorvastatin group received atorvastatin 40mg orally instantly after hospitalization and 20 mg once a day from the second day. The patients received continuous dynamic electrocardiogram detection for 24 hours after PCI and according to the dynamic electrocardiogram outcomes , the early heart rate variability and malignant ventricular arrhythmia were measured and compared between groups. Results (1) Compared with the normal control group, the heart rate variability was significantly reduced, the incidence rate of malignant ventricular arrhythmia was much higher in patients with acute myocardial infarction(P<0.01). (2) Compared with the regular dose atorvastatin group, the heart rate variability was significantly increased(P<0.05), the incidence rate of malignant ventricular arrhythmia was much lower in high dose atorvastatin group after emergency PCI (P=0.03). Conclusion The heart rate variability was significantly reduced and the incidence rate of malignant ventricular arrhythmia was much higher in patients with early acute myocardial infarction; high dose atorvastatin can increase the heart rate variability and decrease the incidence rate of malignant ventricular arrhythmia in patients underwent emergency PCI. Related Articles | Metrics

流行病学调查

Select

The Analysis of Therapy in3012Severe Acne Patients 2014, 42 (4):  366-369 .  Abstract ( 498 )   PDF (355KB) ( 2915 )   [Abstract] Objective To analyze the current clinic application of severe acne treatment, and to provide evidences to improve its treatment. Methods Using sampling survey, a total of3012severe acne patients who visited the dermato? logical department of 35hospitals over the country for the first time were selected for this study. Each patient filled a ques? tionnaire about their acknowledgement, history of medical intervention and drug therapy of severe acne. Results Among all3012patients with severe acne,76.6% believed acne is a kind of skin disease, but only35.2% of the patients went to see doctors at early stage of disease, while others choose interventions such as self-extrudation , topical medication or skin care products, herbal tea /folk recipes, beauty salons and application of coverture cosmetics. Among all severe acne patients, 2388cases (79.3%）had taken oral medications, which included1161(48.6%) patients who took anti-biotics.394cases (33.9%) took roxithromycine and173cases(14.9%) took other kinds of anti-biotics.55.5% of all these patients who took oral medication less than4weeks in duration.2081cases (69.1%) applied external drugs, in which includes90cases (4.3%) of using glucocorticoid, and437cases (21.0%) of using other kinds of external products. The adverse effects of topical treat? ments were commonly observed, such as erythema (512cases,24.6%), desquamation (683cases,32.8%), scab (73cases, 3.5%) and hypersensitive (281cases,13.5%).Conclusion Severe acne is a disease need systematic treatment. but only mi? nority of the patients went to see doctors at early stage of disease. The current problems in treatment of severe acne include lack of target in choosing drugs, not long enough treatment course, and adverse effects of cutaneous administration.Early , safe and targeted medical attention with sufficient treatment course is encouraged. Related Articles | Metrics

Select

Analysis of Seasonal Variations in The Incidence of Primary Acute Cerebral Infarction 2014, 42 (4):  370-373 .  Abstract ( 555 )   PDF (311KB) ( 2902 )   [Abstract] Objective Investigating the relationship between the incidence of Primary Acute Cerebral Infarction (PACI) and seasonal variation to employ measures to prevent PACI with climate change. Methods A retrospective analysis of patients diagnosed with PACI between2009and2013in the department of Neurology of Second hospital of Tian Jin Medi? cal University (n=1198patients) was carried out. Combined with the general clinical data, we analyzed seasonal, monthly and weekly variation among PACI incidents.Results The incidence of PACI increases over years between2009to 2013 （P<0.01）. Significant difference of incidents of PACI was observed between each season (P=0.047). Incidence of PACI peaked in winter（30.33±9.63/month）, while bottomed in spring（21.83±5.36/month）. Significant difference of incidents of PACI was also observed between each months（P=0.010）. The monthly incidence was highest in January and February （33.25±9.62/month）and lowest in March and April（20.75±4.89/month）. The seasonal variation was only found in the pa? tients who are complicated with pulmonary infection (P<0.01) regardless of the presence or absence of other risk factors, such as smoking, drinking ,hypertension, coronary heart disease, and diabetes mellitus. The weekly variation of PACI was on? ly significant in patients younger than 65years old（P=0.043）. The peak incidence among a week was Monday （17.86%）,and incidence bottomed on Friday (13.36%).Conclusion Our study revealed that the incidence of PACI increase over year be? tween 2009to2013and it shew a characteristic variation with respect to season, month and week. Based on these results, we can formulate prevention measures accordingly. Related Articles | Metrics

应用研究

Select

Analysing Factors Causing Pancreatic Fistula post Pancreaticoduodenectomy with External Drainage of Pancreatic Duct 2014, 42 (4):  374-377 .  Abstract ( 710 )   PDF (311KB) ( 2640 )   Objective To analyze the relevant factors of pancreatic fistula post operation of pancreaticoduodenectomy with external drainage of pancreatic duct. Methods A total of 133 patients undergoing pancreaticoduodenectomy with external drainage of pancreatic duct in our hospital from 1999 to 2011 were retrospectively analyzed. Logistic regression analysis was used to analyze the relevance of pancreatic fistula with age, gender, combined diseases, pancreatic duct diameter, pathological types, preoperative total bilirubin(TBIL) and albumin(ALB) levels, drainage of the bile duct before operation, obstruction of the pancreatic duct drainage and postoperative application of growth somatostatin.Then we also analyzed the relationship between the risk factors and the severity of pancreatic fistula. Results Postoperative pancreatic fistula occurred in 24 cases (3 cases of grade A,13 cases of grade B and 8 cases of grade C)among the 133 patients. Logistic regression analysis showed that obstruction of the pancreatic duct drainage is a major risk factor of pancreatic fistula in these patients（OR=4.529，P=0.005）. The patients whose pancreatic duct drainage was obstructed had a significantly higher pancreatic fistula rate than the patients whose drainage was not obstructed (30.8% vs. 12.8%, P<0.05). The occurrence of pancreatic fistula has no significant correlation with age, gender, combined diseases, pancreatic duct diameter, pathological types, preoperative TBIL, ALB level, preoperative bile duct drainage and postoperative application of somatostatin. And in those pancreatic fistula patients, the pancreatic fistula was severity in the obstructed ones than the unobstructed ones.Conclusion The obstruction of the pancreatic duct drainage is a major risk factor of pancreatic fistula after pancreaticoduodenectomy with external drainage of pancreatic duct. If adequate preventive measures were done in operation process to decrease the opportunity of pancreatic duct drainage obstruction, the incidence of pancreatic fistula and pancreatic fistula severity will be significantly reduced. Related Articles | Metrics

Select

Comparative Study of LISS and the Condylar Support Plates in the Treatment of AO Type C Distal Femoral Fractures in Adults 2014, 42 (4):  378-380 .  Abstract ( 521 )   PDF (285KB) ( 2826 )   [Abstract] Objective To summarize complications and early clinical effect of less invasive stabilization system and the femoral condylar support plates in treatment of AO type C distal femoral fractures. MethodsWe reviewed46patients who had internal fixation of AO type C distal femoral fractures. Of all46patients,25were with less invasive stabilization sys? tem and 21were with femoral condylar support plates fixation. Comparative analysis was performed using intraoperative in? dex, postoperative complications and the Evanich score at follow- up. Results All46patients were followed up with a mean time of19.6months after surgery. The difference in incision length, blood loss, fracture healing time was significant between the2groups（P<0.05）but not in the duration of operations and hospital stays（P>0.05）. The statistical signifi? cance was also found in the total incidence of postoperative complications and the Evanich score at the last follow-up（P< 0.05）. Conclusion Patients with less invasive stabilization system fixation had the characteristics of less trauma, shorter fracture healing time, less postoperative complications and better functional recovery compared with femoral condylar sup? port plates. Less invasive stabilization system had became an ideal internal fixation in treatment of AO type C distal femoral fractures. Related Articles | Metrics

Select

The Clinical Research of Different Hyperbaric Ropivacaine Concentrations Used in Lumbar Anesthesia for Elderly Patients undergoing Transurethral Prostatic Resection 2014, 42 (4):  381-383 .  Abstract ( 479 )   PDF (265KB) ( 2715 )   [Abstract] Objective To assess the anesthetic effect of different concentrations of hyperbaric ropivacaine used in lumbar anesthesia for elderly transurethral prostatic resection. Methods We randomly divided58elderly patients who un? derwent transurethral prostatic resection with ASA (American Surgical Association) physical status level Ⅰ- Ⅱ into 2 groups as group A and B, using numeric random table method. Each group includes29cases. After being punctured between L3-L4, patients were injected with 0.5% (group A) and0.75%(group B) hyperbaric ropivacaine (diluted with10% glucose) to the subarachnoid space in a speed of 0.1-0.3mL/s and the overall dose was12.5mg in each case. Then the extents of sen? sory and motor block were compared between two groups, and their anesthesia effect and adverse reactions were also record? ed.Results Compared with group A, onset time of sensory block in group B was significantly shorter (P<0.05), while onset time for motor block was of no significant difference；By contrast to group A, acting time of sensory block in group B pro? longed significantly (P<0.05) while acting time of motor block was of no statistically significant difference(P>0.05);By con? trast to group A, the anesthesia effect of group B was more effective while the adverse reactions of the two groups was of no significant difference (P>0.05). Conclusion 0.75% hyperbaric ropivacaine in lumbar anesthesia for elderly patients un? dergoing transurethral prostatic resection could provide a better anesthesia and analgesia effect than0.5% hyperbaric ropiva? caine without more significant adverse reactions. Related Articles | Metrics

Select

The Application of Chimney Technique in Thoracic Endovascular Aortic Repair of Thoracic Aortic Lesions 1,Minxin Wei 3 2014, 42 (4):  384-386 .  Abstract ( 601 )   PDF (372KB) ( 3069 )     [Abstract] Objective To evaluate the feasibility of strent-graft with Chimney technique in thoracic endovascular aortic repair (TEVAR) of thoracic aortic dissection left subclavian artery (LSA) disease and left common carotid artery (LCCA) disease without good landing zone. Methods A total of21patients with thoracic aortic diseases complicated by in? sufficient proximal anchoring area，who were presented in our hospital in recent years，were selected in this study. The clini? cal data were retrospectively analyzed. The thoracic aortic diseases included aortic dissection (n=11), aortic pseudoaneurysm (n=2), aortic aneurysm(n=1) and penetrating ulcer(n=7). Among all 21patients，lesion was located in distal to LSA in 18pa? tients with distance to LSA anchoring less than15mm,and the lesion was located between the LSA and LCCA in the rest3 patients．Thoracic aortic stent-graft placement was carried out．The ostium of LSA was intentionally and completely cov? ered by thoracic aortic stent-graft and left subclavian artery or left internal carotid artery stent-graft placement was subse? quently performed．The patients were observed for symptoms of cerebral and upper limb ischemia. The postoperative com? plications such as endoleak and the patency of LSA were assessed with angiography.Results Thoracic aortic stent-graft placement was suceessfully carried out in all21patients. In addition，one“Chimney”stent was properly implanted in LSA or LICA in each patient．After the procedure，no complications of nervous system or severe ischemia of upper extremity was observed．Follow-up examinations between to38months after the treatment revealed that the aortic stent-graft remained in stable condition without type I endoleak. Meanwhile the blood flow in“chinney”stent was unobstructed. Conclusion Chimney technique can expand the applicability of TSGP with high tolerance. Chimney technique expand the applicability of TEVAR for patients with challenging anatomy. It is a safe，effective and microinvasive method to treat thoracic aortic lesions. Related Articles | Metrics

Select

MRI Appearances and Clinical Analysis of Lateral Ventricular Subependymomas 1, 2014, 42 (4):  387-389 .  Abstract ( 732 )   PDF (397KB) ( 2823 )   [Abstract] Objective Analysis of MRI image of lateral ventricular subependymomas to improve our recognition of radiology manifestation of the disease.Methods Clinical data and MRI findings of6patients with subependymomas proved by surgical pathology, observed from January2007to June 2013, were analyzed retrospectively. Results Six patients (4 males and2females) presented with headache and dizziness. Among these 6patients, tumor developed in right lateral ventri? cle in three patients, in left lateral ventricle in2patients and in frontal of dual lateral ventricle in one patient. In MRI image, tumors showed iso- intense to hypointense to normal white matter on T1- weighted and DWI images, hyperintense on T2- weighted and FLAIR. Mild or no enhancement was noted in most cases. MRS showed findings consistent with low-grade tu? mor, with a normal choline peak and depressed N-acetyl-aspartate peak.Conclusion Lateral ventricular subependymo? mas have characteristic MRI features and multi-direction images are helpful for diagnosis. Related Articles | Metrics

综述

Select

Recent Research in Role of CD4+CD25+ Regulatory T Cell in Pathogenesis of Immune Thrombocytopenia Hai-Feng ZHAO 2014, 42 (4):  390-392 .  Abstract ( 585 )   PDF (273KB) ( 2842 )   [Abstract] Immune thrombocytopenia (ITP), the most common hemorrhagic disease, is an organ-specific autoimmune disease characterized by decreased platelets count due to auto- antibodies mediating platelets destruction and insufficient platelets production. The etiology of ITP is still not completely known. Regulatory T cells, also called Tregs, are character? ized by CD4 + CD25 + , and positive of transcription factor forkhead box P3. They belong to a subpopulation of T cells special? ized for immune regulation and play an important role in maintaining the immune balance. Decreased production and defect? ed function of CD4 + CD25 + Treg was found not only in the ITP animal model but also in the ITP patients. It indicates that the Treg was involved in the pathology of ITP. This review focus on the characteristic and function of Tregs and their relationship with pathogenesis of ITP. Related Articles | Metrics

Select

Current Study and Progress of Serum Tumor Markers in Lung Cancer 2014, 42 (4):  393-395 .  Abstract ( 516 )   PDF (285KB) ( 3153 )   [Abstract] Lung cancer is one of the most common cancers in the world, but there is no good clinical markers being found to diagnose the disease at early stage and to predict its prognosis. Therefore, many scholars studied on it. In this re? view, we introduced some common and latest clinical serum tumor markers of lung cancer. Related Articles | Metrics

Select

The Application of the Induced Pluripotent Stem Cells Disease Modeling 2014, 42 (4):  396-399 .  Abstract ( 403 )   PDF (308KB) ( 2856 )   [Abstract] Induced pluripotent stem cells (iPSC) technology play an important role in studying diseases in vitro. iPSC disease model was based on the iPSC technology, and has achieved important results in many diseases. For those spe? cific genetic diseases that are relatively easy to simulate, iPSC disease model is especially useful. This technology can direct? ly obtained required material from patients, which is free from ethical constraints and rich in cell sources . iPSC disease model as widely used in studying disease mechanism, drug screening, safety inspection, cell therapy, and so on. It is expect? ed to be used in gene correction, gene repair and to obtain the required target cells for the treatment. Cells modification caused by reprogram, lacking of optimum experimental scheme for cell differentiation and difference in genetic backgrounde limit the application of iPSC disease model. More advanced nonintegrated programming technology, improvement of culture conditions, stricter quality control and setting up the standard for iPSC disease model will help to solve these problems. Related Articles | Metrics