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15 January 2012, Volume 40 Issue 1 Previous Issue    Next Issue
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论著
临床论丛
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Expression of E2F3 and AR in Prostate Cancer and Its Significance 2012, 40 (1):  0-20 .  Abstract ( 1193 )   PDF (1751KB) ( 3614 )   Objective: To investigate the expression level of E2F3、AR in prostate cancer(PCa) and discuss their significance in carcinogenesis and progression of PCa, as well as their relationship with the clinical stage and pathologic grade and their correlation with each other. Methods: The expression of E2F3、AR was detected by immunohistochemistry (IHC) in tissues from 50 cases of prostate cancer and 50 cases benign prostatic hyperplasia (BPH) , The relationship with the clinical stage and pathologic grade and their correlation with each other by was analyzed by SPSS 13.0.Results:1.The rate of positive E2F3 for BPH and prostate cancer was 20%(2/10)and 66%(33/50) respectively. The expression level of E2F3 in prostate cancer was higher than that in BPH(P<0.0001)，and it was related positively with clinical stage(P<0.001)and pathological grade(P<0.020) in prostate cancer. 2. The rate of positive AR for BPH and prostate cancer was 90 %(9/10) and 82%(42/50) respectively. The expression level of AR in prostate cancer was low than BPH(P<0.0001)，and it was related negatively with clinical stage (P<0.0004) and pathological grade(P<0.0005) in prostate cancer.3. The expression level of E2F3 in PCa was uncorrelated with AR expression（rs=-0.017,P>0.05）. Conclusion: E2F3 may play an important role in the onset and progression of prostate cancer by the cell cycle pathway.E2F3 can promote prostatic cell proliferation. Related Articles | Metrics

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Curative effect studies of intrathecal injection methylprednisolone in treatment of multiple sclerosis and the relativity between cost of hospitalization and therapy methods 2012, 40 (1):  0-20 .  Abstract ( 1256 )   PDF (394KB) ( 2005 )   Abstract: Objective To evaluate the relationship in the cost of hospitalization，in hospital day and the effect of Intrathecal injection methylprednisolone for acute multiple sclerosis(MS). Methods 70cases were divided into two groups as intrathecal methylprednisolone group and methylprednisolone impact group，then evaluated the reduction of Kurtzke expanded disability status scale（EDSS）and the rate of side effects at different time after treatment. The average total cost of hospitalization， average cost of hospitalization per day， the proportional of drugs were analysed with the software of SPSS13.0. Results Aerage improvement in mean expanded disability status scale （EDSS）in intrathecal methylprednisolone group ，methylprednisolone impact group and dexamethasone group at 5day were（1.088±0.995）scores and（0.429±0.763）scores ，at 15day were（1.483±1.332）scores and（1.479±1.326）scores，the average costs of hospitalization were （6520.78±1536.26） yuan and （11059.01±2356.32） yuan ， and the average costs of hospitalization per day were （532.22±97.37） yuan and（664.01±105.68） yuan，average hospitalizing days were （12.8±2.3） days and（20.7±3.9） days . Conclusion The improvement of EDSS scale of intrathecal methylprednisolone group were significantily obvious compared with the control group during earlier period of treatment. Intrathecal methylprednisolone therapy worked faster compared with the control group, the average total cost of hospitalization is the and average hospitalizing days is more shorter，the proportional of drugs of intrathecal methylprednisolone group is decrease. The average total cost of hospitalization and average cost of hospitalization per day in methylprednisolone impact group costs are the most ，the proportional of drugs is increase. the average hospitalizing days of methylprednisolone impact group is the longer. Related Articles | Metrics

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Expression and clinical significance of HIF-1α and VEGF-C in Prostate Cancer 2012, 40 (1):  0-20 .  Abstract ( 1249 )   PDF (1780KB) ( 1894 )   Abstract Objective: To study the different expression and clinical significance of hypoxia-inducible factor 1α (HIF-1α) and vascular endothelial growth factor C (VEGF-C) in the prostate carcinoma. Methods: SABC immunohistochemical analysis was used to determine the expression levels of HIF-1α and VEGF-C in 59 cases of prostate carcinoma (PC) and 13 cases of benign prostatic hyperplasia (BPH). The expression and relationship of HIF-1α and VEGF-C with clinic-pathological features were analyzed. Results: The expressions of HIF-1α and VEGF-C were significantly higher in PC (66.1%, 76.3% respectively) compared with BPH (7.7%, 0 respectively). The expressions of HIF-1α and VEGF-C in neoplastic tissue of patients were closely correlated with the tumor differentiation, clinical stages and the lymph node metastasis (P<0.05). Their expressions, however, did not show any correlations to the patient’s age and PSA level (P>0.05). There was a positive correlation between the expressions of HIF-1α and VEGF-C in prostatic carcinoma(r=0.261, P<0.05). Conclusion：The synergy of HIF-1α and VEGF-C may play a great role in the genesis and development of prostate tumor. Maybe the over-expression of HIF-1αinduces tumor invasion by activating the transcription of VEGF-C. Related Articles | Metrics

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The relationship between heme oxygenase-1 promoter polymorphism And hypertension 2012, 40 (1):  0-20 .  Abstract ( 1035 )   PDF (416KB) ( 1902 )   Background: Heme oxygenase-1 is a kind of antioxidative enzyme. A dinucleotide GT repeat in promoter region of Human HO-1 gene shows a length polymorphism that may modulates the level of gene transcription. Therefore this polymorphism phenomenon may associate with the development of essential hypertension. Objective: (1) To investigate the relationship between the length polymorphism of GT repeat in HO-1 promoter area and essential hypertension.(2) To observe if this polymorphism will affect the levels of bilirubin, TNF-? and IL-10 in plasma. (3) To evaluate the effect of the genotypes in the plasma levels of bilirubin, TNF-? and IL-10. Methods: The HO-1 gene GT dinucleotide repeat polymorphism in promoter area was evaluated in 102 EH patients and 134 healthy controls. The genotypes were analyzed by PCR and polyacrylamide gel electrophoresis. Plasma levels of TNF-? and IL-10 were obtained by ELISA methods. Bilirubin levels were measured by automatic chemistry analyzer. Results: (1) The plasma levels of bilirubin, TNF-? and IL-10 in EH group were higher than in control group. (the levels of bilirubin, TNF-? and IL-10 in EH group were 12.53?4.41umol/L; 2.22?0.57ng/ml; 40.87?5.53pg/ml respectively VS control group: 11.46?5.53umol/L; 1.97?0.47ng/ml; 38.17?4.46pg/ml p?0.05 ) (2) The long allele with ≥32 (GT)n repeats (L allele) was found more frequently in hypertension group(26.5% vs 14.6% X2 =10.42, p?0.05). (3) There was no significant difference to be found between the genotypes and the plasma levels of TNF-? and IL-10.The plasma bilirubin levels in the cases carrying with long allele were higher than the cases carrying with short allele. Conclusions: The long allele of HO-1 genotype may be associated with the development of essential hypertension. The levels of bilirubin, TNF-? and IL-10 in plasma may affect the development of EH . Related Articles | Metrics

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The effect of rosiglitazone on the level of serum visfatin and ghrelin in patients with type 2 diabetes 2012, 40 (1):  0-20 .  Abstract ( 1206 )   PDF (306KB) ( 1939 )   【Abstract】Objective To investigate the effect of rosiglitazone on the level of serum visfatin and ghrelin in type 2 diabetes patients．Methods 72 patients with type 2 diabetes were randomly divided into control group and rosiglitazone treated group (36 patients in each group). And patients in rosiglitazone treated group were treated with rosiglitazone (4 mg/d) for 16 weeks. Results After treatment for 16 weeks, the levels of blood pressure, blood glucose, triglyceride (TG) and homeostasis model assessment-insulin resistance (HOMA-IR) were significantly reduced in these two groups. And those in rosiglitazone treated group reduced much more compared with the control group. Increased level of HDL-c and serum ghrelin, and reduced level of serum visfatin were observed in rosiglitazone treated group compared with those before treatment. However, there were no significant differences in the level of those characteristics between before and after treatment in the control group. Pearson analysis shows that the level of serum visfatin was positively associated with body mass index (BMI), fasting plasma glucose (FPG) and HOMA-IR. And the level of serum ghrelin was negatively associated with BMI, FPG, TG and HOMA-IR. Conclusions Rosiglitazone can result in elevated level of serum ghrelin and reduced level of serum visfatin in patients with type 2 diabetes．And this may be associated with the mechanism of improving insulin resistance. Related Articles | Metrics

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Relationship between Microalbuminuria and body mass index 、waist to hip ratio and Waist to height ratio in Patients with Type 2 Diabetes 2012, 40 (1):  0-20 .  Abstract ( 1170 )   PDF (371KB) ( 1973 )   Abstract Objective:To investigate the relationship between microalbuminuria and the indeces of fat. Methods:.Based on the results of the body mass index（BMI）,the 292 type 2 diabetes mellitus(T2DM) patients were divided into normal weight group（88 cases）,overweight group (121 cases)and obesity group(83cases).The differences of 24h urinary microalbumin(UMA) were compared among the three groups,and therefore,the factors that influence UMA were drawed. Results:There were significant differences in the values of BMI、waist-to-hip ratio(WHR)、waist –to-height ratio(WHtR)、UMA、systolic pressure(DBP) among three groups（P<0.01 or P<0.05）；Correlation analysis showed a positive correlation between UMA and the duration of the disease, WHtR,WHR,BMI,SBP,DBP(P<0.01 or P<0.05).Multiple stepwise regression analysis showed WHtR,SBP were independent risk factors of UMA(P<0.05 or P<0.01). Conclusion:The possibility of having UMA was significantly increased in T2DM patients with higher WHtR;The abdorminal obesity is an risk factor of UMA in T2DM patients. Related Articles | Metrics

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Analysis of excretions in urine of metformin-glipizide tablet after oral administration in healthy human 2012, 40 (1):  0-20 .  Abstract ( 1163 )   PDF (855KB) ( 1908 )   ABSTRACT OBJECTIVE To develop methods for the determination of metformin and glipizide in human urine and to study the urinary excretions of drugs in healthy Chinese volunteers after single dose administration. METHODS After metformin urine samples were diluted by mobile phase, the supernatant was injected into the system directly by using Lichrospher ODS coLumn with a mobile phase of 3 mmoL/L sodium dodecylsulfate (pH 4)∶acetonitrile (75∶25),and the detection wavelength is 240 nm. Glipizide and IS were extracted by acetic ether after urine acidation, and separated on Lichrospher ODS column using acetonitrile and 0.1% acetic acid (10 mmoL?L-1ammonium acetate) (55∶45) as mobile phase. MS was performed in the selected anion monitoring mode. Ten health Chinese volunteers were treated with single dose of two metformin-glipizide tablets, the metformin and glipizide concentrations in urine were determined by the methods. RESULTS The inter-day and intra-day precision values were below 10%. In 24 h，the accumulation amounts of metformin and glipizide excreted in urine were 102.5 ± 27.3 mg、452.2 ± 191.4 μg, respectively, the accumulated excretion rates were 26.27 % ± 7.0 %, 9.04 % ± 3.83 %, respectively. CONCLUSIONS The assays have been proved to be accurate and sensitive, and can be used in the study of human urinary excretions of glipizide and metformin. Related Articles | Metrics

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The preliminary study of relationship between donor glomerular filtration rate and post-transplant graft function in living kidney transplantation 2012, 40 (1):  0-20 .  Abstract ( 828 )   PDF (362KB) ( 1915 )   Abstract: Objective: To investigate the relationship between donor glomerular filtration rate and post-transplant graft function in living kidney transplantation Methods: A retrospective analysis of 125 cases of living donor kidney transplantation (without acute rejection or delayed graft function, follow-up for 2 years) ,donor estimated glomerular filtration rate ,recipient estimated glomerular filtration rate at 1week and1 ,2 years post transplantation. Using the correlation method to analyze the relationship between them. Using multiple linear regression analysis of he relationship between donor weight, donor age, donor GFR, recipient age, recipient weight and recipient GFR at 1 year post transplantation . Results: The GFR of recipients at 1week,1 ,2 years post transplantation were significant correlated with the donor GFR(r=0.217 p=0.015,p = 0.039 and p = 0.003 respectively ). According to multiple stepwise regression analysis, donor GFR and recipient body weight were predictors of grft function at 1 year post transplantation, the linear regression equation : recipient GFR at 1 year post transplantation=79.9－0.49×recipient body weight＋0.24×donor GFR。 Conclusion: The donor GFR was the important characteristic for predicting the grft function in living kidney transplantation Related Articles | Metrics

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The expression and significance of HBeAg in liver failure and chronic hepatitis B 2012, 40 (1):  0-20 .  Abstract ( 1165 )   PDF (333KB) ( 1897 )   Abstract Objective: To explore the expression of HBeAg and the relationship of HBeAg and serum HBV-DNA in chronic hepatitis B and liver failure. Methods: we collected 118 patients with Chronic Hepatitis B (CHB) and 109 patients with liver failure from Tianjin Infectious Diseases Hospital in April 2007 to September 2010，and analyzed retrospectively the changes of HBV-DNA content, the proportion of HBeAg-positive and-negative patients and the HBV-DNA content changes of HBeAg-positive and-negative patients .Results:in liver failure group the proportion of HBeAg-negative patients is higher than the proportion of CHB group, and the HBV-DNA level of HBeAg-negative patients is lower than the positive; the HBV-DNA level of liver failure group is lower than CHB group, and the HBVDNA levels of HBeAg positive and negative patients with liver failure are lower than the corresponding group of patients with CHB; In liver failure group, the proportions of HBeAg-negative patients are no significant difference , the HBV-DNA levels of patients whit Chronic liver failure and early acute-on-chronic liver failure are lower than the late acute-on-chronic liver failure ,but the HBV-DNA level of medium and late acute-on-chronic liver failure is no significant difference. Conclusion: different expression of HBeAg may play an important role in the evolution of chronic hepatitis B and liver failure, but it does not related with the severity of illness. Related Articles | Metrics

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Comparison of Two Strategies to Minimize Ventricular Pacing 2012, 40 (1):  0-20 .  Abstract ( 1019 )   PDF (343KB) ( 1941 )   Objective: Managed ventricular pacing (MVP) and Search AV+ are representative dual-chamber pacing algorithms for minimizing ventricular pacing(VP). This study is to examine the difference in ability to reduce percentage of VP in patients of sick sinus syndrome who have been planted with pacemaker between these two algorithms. Methods: Subjects were randomly divided into the MVP group and Search AV+. The percentages of ventricular pacing of the patients were compared one month after planting pacemaker. Results: The median of percentages of VP were lower during the MVP operation than that during the Search AV+（0.3% vs 3.1%，P<0.01）. The percentages of atrial pacing have no significant difference between two groups. Conclusions: Managed ventricular pacing algorithm, compared with Search AV+, offers further percentage of VP reduction in patients of sick sinus syndrome with a dual-chamber pacemaker. Related Articles | Metrics

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The clinical analysis of non-coronary artery disease patients with chest pain in the different gender 2012, 40 (1):  0-20 .  Abstract ( 1098 )   PDF (322KB) ( 1984 )   Abstract Objective: To analyze the difference of clinical features, etiology, medication, misdiagnosed, and negative emotions in the different sexual non-coronary patients with chest pain . Methods: The CAG / CTCA and SPECT examination confirm the diagnosis in patients with non-coronary chest pain,and these patients were divided into male and femal groups by sex . The two groups were Summarized and analyzed in the clinical features, misdiagnosis, etiology, drug use and mental status. Results: 141 cases patients of non-coronary heart disease patients included 85cases females and 56 cases males. The female patients were misdiagnosed for a longer time. The onsetal age of female patients were earlier than males ,and more likely associated with radiation of pain and ECG ST-T changes. The commonal cause of female patients were dued to functional diseases of social and psychological factors . (p <0.05). The mindiagnosis of female patients didn’t timely perform CAG / CTCA examination by themselves. And the male patients didn’t delay diagnosis by obeying the doctor suggestion. It was excluding coronary heart disease before that the using medication proportion of female patients was significantly higher than male patients. after excluding coronary heart disease, 91.1%women and 100% of men stopped taking drugs.The patients had particularly negative emotions by the SDS, SAS score before exclusion of coronary heart disease,and after excluding coronary heart disease,the negative emotions decreased (p <0.01),which had no significant difference between different genders. Conclusion: The clinical characteristics of non-coronary heart disease patients with chest pain is significant gender differences. The female patients are more likely long-term misdiagnosis.Once we highly suspected patients with coronary heart disease,we should make them do CAG / CTCA examination as early as possible, in order to prevent drug abuse and reduce the negative emotional and economic losses. Related Articles | Metrics

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Influence on internal adaptation and retention of Ni-Cr based crown with different coating layers of diespacer 2012, 40 (1):  0-20 .  Abstract ( 1117 )   PDF (445KB) ( 1983 )   Abstract Objective: This study was to evaluate internal adaptation and retention of Ni-Cr based crown with different coating layers of diespacer. Methods: A total of 30 intact human first premolars extracted freshly were collected. After tooth preparation，30 dies were made and randomly divided into 3 groups according to different coating layers of diespacer．Ni-Cr alloy was selected to fabricate based crowns which were cemented to teeth with polyether impression material to measure the internal adaptation．Then the samples were made of the crowns and teeth bonded with Fuji glass ionomer. After immersed in artificial saliva for a month and subjected to 10000 cyclic mechanical loading，the samples were sent to measure the maximum load at failure．Results: The internal adaptation at the three groups with different layers of diespacer had significant difference（P<0.01）；For the maximum load at failure，no significant difference was found between one layer group and two layers group（P>0.05），while both of them had significant differences compared with three layers group（P<0.01）．Conclusion: Coated with one or two layers of diespacer on the die could improve internal adaptation and retention of Ni-Cr based crown significantly. Related Articles | Metrics

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The relationship between CAG polymorphisms of androgen receptor gene and acute myocardial infarction in men 2012, 40 (1):  0-20 .  Abstract ( 605 )   PDF (367KB) ( 1936 )   Objective To explore the relationship between CAG polymorph- isms of androgen receptor gene and acute myocardial infarction in men. Methods 112 acute myocardial infarction patients and 118 healthy men were selected. The length of CAG repeats in the peripheral blood AR gene was examined by PCR and direct sequencing. Results For the AMI patients and the controls, the numbers of CAG repeats ranged from 12-33,with mean values 23.09±3.520 and 20.33±3.654,respectively.There were statistically significant differences between two groups. Men with CAG repeats length of 23 or longer may be in high risk of AMI(odds ratio,4.479 ；95% CI 2.564-7.824，P<0.001),compared with men with CAG repeats length shorter than 23.Conclusions The CAG repeat polymorphism in the androgen receptor is associated with acute myocardial infarction, Men with CAG repeats length of 23 or longer may be in high risk of acute myocardial infarction . Related Articles | Metrics

临床论丛

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Explore the Relative Factors of Restenosis and Occlusion After Artificially Grafting Femoropopliteal Bypass 2 2012, 40 (1):  0-20 .  Abstract ( 1095 )   PDF (309KB) ( 1937 )   Abstract Objective: To explore the relative factors of restenosis and occlusion after artificially grafting femoropopliteal bypass on chronic ischemia of low extremities in different postoperation time. Methods: A total of selected 115 cases with peripheral artery occlusive disease(PAOD), who were treated in our hospital from Mar. 2006 to Mar. 2008, were divided into occlusive group and patent group according to their grafting patency in different postoperation time. Complications and run-off way were evaluated. Results: Diabetes mellitus were significantly higher in the occlusive group at 24 months(57.1%, P<0.05). The rates of the patients who had only one run-off artery which wasn’t serious stenosis or occlusive were 38% in occlusive group and were 19.2% in patent group at 24 months. There were significant difference between 2 groups(P<0.05). Conclusion: Diabetes mellitus and only one run-off artery which wasn’t serious stenosis or occlusive maybe the major high risk factors that lead to long-term artificial graft obliteration． Related Articles | Metrics

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Correlations between NF-κBp65 in Peripheral Blood Mononuclear Cells and High Sensitive C-reactive Protein and Malnutrition in Hemodialysis Patients Jing-Fu WANG 2012, 40 (1):  0-20 .  Abstract ( 1268 )   PDF (364KB) ( 1934 )   Objective: To investigate the correlations of NF-κBp65 activity in peripheral blood mononuclear cells(PBMC) with high sensitive C-reactive protein(hs-CRP) levels and malnutrition in patients with maintenance hemodialysis.Method: 71 MHD patients were treated in our hospital from June in 2010 to May in 2011, whose treatment period was longer than 3 months and the Urea remove index(Kt/V) was greater than 1.2. MHD patients with malnutrition were 44 cases and who with good nutrition were 27 cases.The Subjective Global Assessment of nutrition(SGA),anthropometric indicators, blood biochemistry of 71 patients with MHD were detected. ELISA method was used to test the activity of NF-κBp65 in PBMC. Immune turbidimetric method was used to test the serum levels of hs-CRP. Bivariate correlation analysis was measured with Pearson linear correlation or Spearman rank correlation analysis. After controlling age, gender, height, weight, Partial correlation analysis was used for multiple factors analysis.Results:The patients with malnutrition were 44 cases in 71 MHD patients, accounting for 61.97%. The NF-κBp65 absorbance value and the hs-CRP levels in malnutrition group were higher than that of good nutrition group(P<0.01). The normalized protein catabolic rate (nPCR), hemoglobin (HB), serum albumin(ALB), serum prealbumin(PA), serum creatinine (Scr), blood urea nitrogen(BUN), triceps skinfold(TSF), mid-arm muscle circumference (MAMC), mid-arm circumference (MAC), Body mass index (BMI), gripping strength (GS) in malnutrition group were lower than that of the good nutrition group(P<0.05 or P<0.01).SGA value was positively correlated with NF-κBp65 activity and hs-CRP levels (P<0.01). NF-κBp65 activity was positively correlated with hs-CRP levels and age(P<0.01 or P＜0.05), and was negative correlated with HB,ALB,PA（P＜0.01).The hs-CRP levels was significantly negatively correlated with the HB, ALB, PA, TSF,MAMC, MAC（P＜0.05 or P＜0.01). Controlling the variables of age, gender, height and weight, the correlation of NF-κBp65 and hs-CRP with some other parameters associated with nutrition was under partial correlation analysis. The results showed that SGA value was positively correlated with NF-κBp65 activity and hs-CRP levels (P<0.01).NF-κBp65 activity was positively correlated with hs-CRP levels (r=0.777，P<0.01),NF-κBp65 activity and hs-CRP levels were negative correlated with HB,ALB,MAMC,MAC（P＜0.05 or P＜0.01).NF-κBp65 activity was negatively correlated with the PA（P＜0.01),The hs-CRP levels was not correlated with the PA.Conclusions: The incidence rate of malnutrition was higher in MHD patients.The malnutrition could be found earlier by NF-κBp65 activity than hs-CRP levels. Related Articles | Metrics

实验研究

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Effect of Probucol on Atherosclerosis of New Zealand Rabbits 2012, 40 (1):  0-20 .  Abstract ( 1201 )   PDF (10411KB) ( 3912 )   Objective:To investigate effect of probucol on serum lipid, mast cells and atherosclerosis of New Zealand rabbits with normal diet or high fat /high cholesterol diet. Methods:Forty male New Zealand rabbits were respectively supplied with normal fodder, normal fodder and probucol, high fat /high cholesterol fodder, high fat /high cholesterol fodder and probucol, then observed for 4 weeks. The serum lipids were measured. Mast cells were detected by toluidine blue. Aorta atherosclerotic lesion area was measured with Sudan IV. Results:There was a significant decrease in serum triglyceride, total cholesterol, low density lipoprotein cholesterol and high density lipoprotein cholesterol(P<0.05), the percentage of degranulated mast cells in aorta and in jejunum (P<0.001), aorta atherosclerotic lesion area (P<0.001) in high fat /high cholesterol fodder and probucol group than in high fat /high cholesterol fodder group. Conclusion:Probucol can decrease serum triglyceride, total cholesterol, low density lipoprotein cholesterol and high density lipoprotein cholesterol, the percentage of degranulated mast cells in aorta and in jejunum, aorta atherosclerotic lesion area of New Zealand rabbits with high fat /high cholesterol diet. Related Articles | Metrics

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Comparison Of Liposome And Adenovirus Vectors For HGF Gene Transfer To Endothelial Progenitor Cells Sun Ning 2012, 40 (1):  0-20 .  Abstract ( 1045 )   PDF (37268KB) ( 1756 )   Objective: To compare the efficiency of liposome and adenovirus vector for hepatocyte Growth Factor (HGF) gene transfer to Endothelial Progenitor Cells (EPCs). Methods:1.Rat bone marrow-derived EPCs were separated by density gradient centrifugation and cultured, and identified by observing the experiment of uptaking DiL-acLDL and combining FITC-UEA-1 and analyzing the expression of CD133+ by Flow Cytometry. 2.Liposome mediated gene transfection: EPCs were divided to three group: pIRES2-EGFP-HGF group，pIRES2-EGFP group and blank control.3.Adenovirus mediated gene transfect- ion: EPCs were divided to three group: pAdxsi-GFP-HGF group, pAdxsi-GFP group and blank control. 4.The expression of green fluorescent protein (GFP) were observed by fluorescence microscope.The expression of HGF were detected by ELISA ,The proliferation were detected by MTT. Results: Liposome and adenovirus could deliver HGF gene marked by the green fluorescence protein (GFP) to EPCs. Adenovirus proved most effective with efficiencies of up to 80% with higher levels HGF expression and lower levels of cell death. Conclusion: Comparing the liposome, adenovirus vector for HGF gene transfer to EPCs has more efficiency, lower cell toxicity and higher HGF expression. Related Articles | Metrics

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The Expression of Midkine and HGF in Tubular-interstitial of a Rat Model of Type 2 Diabetic Nephropathy limei ZHANG, 2012, 40 (1):  0-20 .  Abstract ( 1307 )   PDF (1867KB) ( 1866 )   Objective: To observe the expression of midkine and HGF in the renal tubles of diabetic rats and to investigate their relationships .Methods:sixty male Sprague-Dawlry rats were randomly divided into two groups:normal control group(n=24)and diabetes mellitus group(n=36).Type 2 diabetic nephropathy were induced by high-glucose and high-fat diet and intraperitonesl STZ after unilateral nephritic excision. At the end of 6th week and the 12th week after diabetes mellitus model established,blood glucose and 24 hours urine protein were determined,and body weight were weighed,and then rats were killed to collect the serum and kidney.We calculated the ratio of kidney weigh to body weigh(KW/BW). The tubular structural changes were examined with HE staining and periodic acid-silver methenamine staining at 6th week and the 12th week. The protein expression of midkine and HGF intubular-interstitial were determined by immunohistochemistry.Results:(1) FBG,KW/BW and 24 hours urine protein were obviously increased in the diabetes mellitus group compared with normal control group(P<0.05).(2) There were severe structural alterations of tubular-interstitium in diabetes mellitus group .(3) At the end of the 6th week, the12th week after diabetes mellitus model established,immunohistochemistry revealed a significant increase in the expression of MK and HGF in the kidney in diabetes mellitus group compared with normal control group(P<0.01). At the end of the 12th week , the expression of MK was obviously increased In diabetes mellitus group compared with the 6th week(P<0.05), the expression of HGF was obviously decreased In diabetes mellitus group compared with the 6th week(P<0.05).(4) In diabetes mellitus group,there were negative correlations between midkine and HGF(r=-0.525,P<0.01).Conclusion: The expression of midkine and HGF in tubular-interstitium are unbalanced,which possibly participated in pathogenesis of diabetic nephropathy. Related Articles | Metrics

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A study on changes of antibrain-antibody and interleukin-1βin rabbits with traumatic brain injury after drainage of cerebrospinal fluid 2012, 40 (1):  0-20 .  Abstract ( 1107 )   PDF (381KB) ( 1867 )   Objective To study changes of antibrain-antibody (ABAb)and interleukin-1β in rabbits with traumatic brain injury with drainage of cerebrospinal fluid therapy and to explore the protective effect of this therapy in rabbit—TBI—models established by lateral fluid percussion machine. Methods 32 rabbits were divided into four groups randomly (16 in control group, 16 in CSF drained group). Rabbits in control group were drained CSF and injected into cistern; rabbits in CSF drained group were drained CSF only. 2 groups rabbits were drained CSF 1ml everyday until a week and phlebotomized and tested with ELISA to observe the changes of IL-1βand ABAb at the time points 1 d, 3 d, 7d, 14d, 21d. Results Levels of IL-1βand ABAb in CSF drained group were lower than that in control group(P<0.05). The mortality rate and disability rate of CSF drained group were lower than those of control group, while the cure rate was the higher than that of control group. Conclusion Drainage of cerebrospinal fluid can help to decrease levels of antibrain-antibody and interleukin-1β. Drainage of cerebrospinal fluid therapy has protective effect on immunologic injury after traumatic brain injury. Related Articles | Metrics

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The change of TNF-αexpression in myocardiac tissue of the spontaneous hypertension rats by oral spironolactone 2012, 40 (1):  0-20 .  Abstract ( 819 )   PDF (331KB) ( 1955 )   Abstract :Objective 　To investigate the effects of oral spironolactone on expression of TNF-αin the myocardiac tissue of spontaneous hypertension rats during t he progressing stage of hytertension. Methods 　Twenty eight 2-weeks- old male spontaneous hypertension rats (SHR) were divided randomly into spironolactone group (SHR-SPIRO , n = 10),and control group (SHR-CON , n = 10) ,sex - age matched Wistar Kyoto rats (WKY group , n = 7) which having normal hypertension were also used as the third group . The spironolactone was solved with distilled water and the animals were weighed once weekly to adjust for drug dosage（20mg/kg.d）.Evaluation were performed after 16 weeks of treatment.The rats were anesthetized at 24-week-old with 1g/kg urethane intraperitoneal. The systolic pressure,diastolic pressure, mean arterial blood pressure were deteced by a tube that was pierced into the carotid artery that was connected with physiological function experimental system. The hearts were rapidly isolated and were cut into two parts. The one was fixed in 10% buffered for immunohistochemistry；The other one was throwed quickly into liquid nitrogen . The TNF-αlevels of left ventricular homogenate were deteced by enzyme linked immunosorbent assay. Results The systelic blood pressure,diastolic blood pressure and mean arterial pressure had no differences in the rats between SHR-SPIRO group and SHR-CON group. (SHR-SPIRO: 174.64±19.6；121.65±19.45；148.4±18. 5 vs SHR-CON: 184.19±8.55；136.76±22.06；150.5±24.5 p＞0.05).but the artery blood pressure of the two groups are higher than the WKY group. The concentration of TNF-α is higer in myocardiac tissue in the SHR-CON group than WKY group(24.52±3.36 vs 1.53±0.21 p＜0.05 ); TNF-α expression decrease significently in SHR-SPIRO group than SHR-CON group(5.9±0.96vs 24.52±3.36 p＜0.05 ).Conclusion 1. The levels of myocardial tissue of SHR is higer than the normal hypertension WKY rats;2.Oral spironolactone can decrease concentration of TNF-α of myocardial tissue in hypertension rats. Related Articles | Metrics

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The Protective Effect of Sodium Ozagrel Lung Ischemia-Reperfusion Injury in Rat 2012, 40 (1):  0-20 .  Abstract ( 982 )   PDF (888KB) ( 2011 )   Objective:To investigate protective effect of Ozagrel sodium on limb ischemia-reperfusion injury of lung in rats.Methods:60 SD rats were randomly divided into 3 groups:the normal control group,the limb-ischemia-reperfusion group,the Ozagrel sodium group. The model rats which underwent 4 hours ischemia and 2 hours reperfusion of hind limbs were made.The histological and ultra-structure changes were observed under light microscope and electron microscope, respectively. TXB2 and PGI2 levels in serum were separately measured by radio-immunoassay. We observed changes of MDA SOD XOD in lung tissue of different groups by colorimetry and observed changes of IL-8 and TNF-α in lung tissue of different groups by avidinbiotincomplex-ELISA. Results: Compared with the limb-ischemia-reperfusion group, The damage in lung tissues of the Ozagrel sodium group alleviate under optical microscopy and electron microscope.The levels of TXB2 and 6-keto-PGFla in serum decreased very significantly. The level of SOD was increased during reperfusion and the values of XOD and MDA were all decreased significantly. The levels of IL-8 and TNF-α in lung tissue were much lower. Conclusions: Ozagrel sodium can alleviate limb ischemia-reperfusion injury of lung in rats. Related Articles | Metrics

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Analysis of clinical characteristics and risk factors for death of aortic dissection 2012, 40 (1):  0-20 .  Abstract ( 1324 )   PDF (293KB) ( 1940 )   Objective Analysis of the clinical characteristics of aortic dissection and to discuss its risk factors for death. Methods Retrospective analysis of the aortic dissection cases in our hospital form 2006 to 2010, using Logistic regression analysis of the relationship between gender, age, relevant clinical characteristics and hospital mortality of aortic dissection. Results The total mortality was 17.5% during hospitalization, in-hospital mortality of DeBakey typeⅠ,Ⅱ,Ⅲ are 7/16（43.8%）,2/8（25.0%）, 2/39（5.1%）, respectively; Logistic regression analysis revealed that pericardial effusion (P=0.001) is a risk factor predicting the death of aortic dissection. Conclusion Pericardial effusion is a independent risk factor for aortic dissection. Related Articles | Metrics

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Clinical analysis of interventional treatment on the bronchosocpy fistula by electronic bronchosocpy 2012, 40 (1):  0-20 .  Abstract ( 1192 )   PDF (309KB) ( 1893 )   Objective:To observe the role of Compont Medical Adhesive to the bronchosocpy fistula(BPF) patients infused by electronic bronchosocpy. Methods:In our study, 23 BPF patients were treated by infused Compont Medical Adhesive by electronic bronchosocpy.The fistula were from 2 to 10mm,and were 4.1mm at average.All patients continued to strengthen anti-infection and supportive treatments at postoperative.Results: We reviewed all patients by electronic bronchosocpy.The 15 patients which their fistula were less than 4mm were healed. Four of the 5 patients which their fistula were from 4 to 7mm were healed by 2 to 3 times treatment.One of their 5 patients was invalid. And only one of 3 patients which their fistula were from 7 to 10mm was healed by several times treatment. Two of their 3 patients were invalid.Conclusion: The treatment of Compont Medical Adhesive infused by electronic bronchosocpy to the BPF patients is a simple,little injury,safe and effective method to and can be preffered. Related Articles | Metrics

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The application of nitrous oxide in Colonoscopy 2012, 40 (1):  0-20 .  Abstract ( 1111 )   PDF (298KB) ( 1937 )   Objective: To compare the effects of nitrous oxide and intravenous anesthesia with propofol in colonoscopy. Methods :103 patients were randomly divided into two groups:the propofol intravenous anesthesia group( group A) and the nitrous oxide group(group B). Observe and compare the blood pressure、pulse oxygen saturation and the performing time of colonoscopy and the time of recovery and the rate of adverse reaction of the two groups. The datas between two groups were compared and statistically analyzed with software SPSS 11.5. Results :group B has shorter recovery time compared with group A,the difference was statistically significant （P<0.05）;the A,B group has no significant difference in vital signs before and after examining and discomfort (P> 0.05). Conclusion: Both nitrous oxide inhaled and propofol intravenous anesthesia can all relieve discomfort in colonoscopy, further more using nitrous oxide has shorter recovery time. Related Articles | Metrics