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15 December 2011, Volume 39 Issue 12 Previous Issue    Next Issue
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论著
实验研究
短篇论著
临床论丛
短篇与病例报告


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论著

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Comparative Study on Different Mesenchymal Stem Cells Differentiate into Nerve-Like Cells in Vitro 2011, 39 (12):  1091-1094 .  Abstract ( 1388 )   PDF (2981KB) ( 2237 )   Abstract Objective：To study the capacity of different mesenchymal stem cells (MSCs) to be differentiated into nerve-like cells in vitro. Methods: The fetal derm-derived MSCs (group A), fetal bone marrow-derived MSCs (group B) and adult bone marrow-derived MSCs (group C) were separated and cultured in expanded medium. The morphology and doubling time of MSCs were detected by inverted microscope. The phenotype and cell cycle of MSCs were detected by flow cytometry. All-trans-retinoic-acid (ATRA) was used to induce three different MSCs to differentiate into nerve-like cells. The differentiated cells were observed by phase-contrast microscopy and detected by immunocytochemistry for neuron-specific marker-neurofilament (NF) and astrocyte specific marker-glial fibrillary acidic protein (GFAP). Results：Our results showed that MSCs derived from three groups were similar in phenotype， morphology and cell cycle. However, the abilities of expansion and passage of MSCs derived from C group were lower than that of MSCs derived from A and B group. After treatment with ATRA, the generations of the three different MSCs group showed typical morphological characteristic of nerve cells. Results of immunocytochemistry staining and Western blot showed that the differentiated nerve-like cells expressed NF and GFAP. Conclusion：Three different sources of MSCs can be induced into nerve-like cells in vitro. But, MSCs in different development stage have different ability of being differentiated into nerve-like cells in vitro. Related Articles | Metrics

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The association between Gene Polymorphisms of N5, 10-Methylenetetrahydrofolate Reductase and Hui Nationality Patients with Essential Hypertension in Ningxia 2011, 39 (12):  1095-1098 .  Abstract ( 1457 )   PDF (2988KB) ( 2171 )   Abstract Objective: To investigate the association between C667T point mutation of N5, 10-methylenetetrahydrofolate reductase (MTHFR) and Hui nationality patients with essential hypertension in Ningxia. Methods: The study was performed in 146 patients with essential hypertensive (EH) and 112 normotensive (NT) control subjects. Blood samples of two groups were collected and then DNA was extracted. MTHFR gene polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The frequencies of three genotypes (CC, CT and TT) showed statistics differences between EH group and NT group (P<0.05). The frequency of genotype 677TT and T allele were significantly higher in patients of EH group than those of NT group (P<0.05). There was no significant difference in diastolic blood pressure between CC genotype group and CT/TT genotype group (P>0.05), but there were significantly higher systolic blood pressure, pulse pressure and mean arterial pressure in CT/TT genotype group than those of CC genotype group (P<0.05). Binary stepwise logistic regression analysis showed that CT+TT genotype, female, body mass index (BMI), triacylglycerol (TG) and urea nitrogen (BUN) were independent risk factors for essential hypertension. Conclusion: There is a correlation between MTHFR C677T gene polymorphism and essential hypertension in patients of Ningxia Hui nationality. The T allele may be the susceptible gene of essential hypertension in Ningxia Hui nationality. Related Articles | Metrics

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Study on primary azoospermia of Y chromosome AZF microdeletions in Tianjin area 2011, 39 (12):  1099-1101 .  Abstract ( 1414 )   PDF (2139KB) ( 2225 )   Related Articles | Metrics

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The application of collagen gel droplet culture drug-sensitivity test (CD-DST) in postoperative chemotherapy of gastric cancer 2011, 39 (12):  1102-1104 .  Abstract ( 1641 )   PDF (2145KB) ( 2132 )   Objective: Observe and value the efficacy of postoperative chemotherapy for gastric cancer under the guidance of CD-DST Technology. Discuss its guiding role and feasibility for chemotherapy of gastric cancer. Methods: The patients who have been confirmed to be effective in vitro with FOLFOX4 by CD-DST enter pilot group， and the patients who have accepted FOLFOX4 without CD-DST enter control group. Compare postoperative one year and three years survival rate between the two groups. Results: In pilot group, year survival rate was 93.5%; three-year survival rate was 83.9%. In control group, year survival rate was 88.6%; three-year survival rate was 60.0%. The difference of three-year survival rate between two groups is significant in statistics. (P﹤0.05) Conclusion: CD-DST technology has some significance for the choice of chemotherapy program for individual treatment of gastric cancer patients. Related Articles | Metrics

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Analysis and discuss of Prognostic Factors for pediatric Rhabdomyosarcoma 2011, 39 (12):  1105-1107 .  Abstract ( 1333 )   PDF (2172KB) ( 2387 )   [Abstract] Objective To explore the clinicopathological characteristics and prognostic factors of pediatric Rhabdomyosarcoma by the retrospective study of clinical and survival data of 93 patients,who were diagnosed RMS.Method The clinical datain cluding sex,age,tumor size,primary site, histological subtypes, COG-staging,COG-grouping,risk classification and surgery were analyzed. We used multi-variety analysis with COX regression model to identify the independent prognosis factors,Influence of the above factors on the survival rate was analyzed by Kaplan-Meier curve and Log-rank test. Results Survival rates of 1-,3-and 5-year in pediatric were 90.3%,62.0%,43.1%,respectively,and the median overall survival time was 19.43 months. Multivariable analysis showed that the histological subtypes, primary site , COG-grouping and surgical resection are independent prognosis factors.The survival analysis showed that there were significant ststistical differences in the prognostic differences of the histological subtypes（P＜0.001）, primary site （P＜0.001）, COG-grouping（P＜0.001） and surgical resection（P＜0.001）.Conclusion The independent prognosis factors of pediatric Rhabdomyosarcoma including histological subtypes, primary site,COG-grouping and surgical resection.It can be used to predict the prognosis and provide the basis data for clinical treatment. Related Articles | Metrics

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Research of the level and relationship of the gastric cancer with VEGF-D and MLVD 2011, 39 (12):  1108-1111 .  Abstract ( 1399 )   PDF (2891KB) ( 2181 )   Objective: To detect the amount of VEGF-D (vascular endothelial growth factor-D ) and MLVD(micro-lymphatic vessel density) in the gastic cancer, analyze the relationship of VEGF-D and MLVD with the following factors: age, gender, clinical stage，lymph node metastasis，histo-differentiation and explore the use of antilymphangiogenic treatment. Methods: 52 samples of the gastric cancer tissue and bordeline tissue of cancer were gathered . 52 samples of normal tissue at least 5cm away from the tumor were also collected. We adopted an immunohistochemistry method to determine the levels of VEGF-D and MLVD in the samples. This was accomplished by bingding the clinical data and stastistical analysis to find how much VEGF-D and MLVD were present in the gastric cancer and normal tissue and the relationship with age, gender, clinical stage ，lymph node metastasis ，histo-differentiation . Results: The expressive rate of VEGF-D in gastric cancer and the normal tissue adjacent cancer had significantly difference (P<0.01 ). The positive rate of VEGF-D in gastric cancer in the samples which had lymph node metastasis was significantly higher than which had no lymph node metastasis（P<0.01 ). The positive rate of VEGF-D in gastric cancer of stage Ⅲ、Ⅳ was significantly higher than that in stage Ⅰ、Ⅱ（P<0.01）. The expression of VEGF-D had no significantly different from poor and well differentiation（P>0.05）. The expression of VEGF-D had significant correlation with lymph node metastasis ( P<0.01). The positive rate of VEGF-D was also correlated with the clinical stage（P<0.01）. The MLVD in the normal tissue was significantly higher than that in the gastric cancer ( P<0.01 ). The MLVD in tumor borderline was higher than that in the normal tissue and gastric cancer ( P<0.01). The MLVD average value in tumor borderline was significantly increased in the VEGF-D positive group than the negative group ( P<0.01). No significantly difference in the MLVD of VEGF-D positive group with the MLVD average in negative group（P>0.05）. Conclusion:The expression of VEGF-D in the gastric cancer is high, and it has correlation with clinical stage, lymph node metastasis. It suggests that the expression of VEGF-D is important in promoting tumor growth and metastasis. MLVD in the tumor borderline was significantly higher than in gastric cancer and normal tissue . The increase of MLVD may be related to the invasion and metastasis of gastric cancer in the tumor borderline. The detection of VEGF-D and MLVD in the tissue of gastric cancer can be accepted as important affiliated markers to evaluate the lymph node metastasis. It may prove to be markers to predict the malignant biology of gastric cancer and prognosis. Related Articles | Metrics

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Prognostic significance of increased postprocedural tumor necrosis factor-α and high sensitivity C-Reactive Protein in acute myocardial infarction patients treated with percutaneous coronary intervention. 2011, 39 (12):  1112-1115 .  Abstract ( 1514 )   PDF (2879KB) ( 2486 )   Objective The aim of the study was to evaluate the prognostic value of the postprocedural tumor necrosis factor-α (TNF-α) and high sensitivity C-Reactive Protein(hs-CRP) in patients with first acute myocardial infarction(AMI) treated with successful percutaneous coronary intervention(PCI). Methods A total of 152 consecutive AMI patients underwent successful PCI were enrolled. TNF-α and hs-CRP were measured within 12 hours after PCI. All patients were followed up for 1 year. Logistic regerssion analysis was used to evaluate predictive values of postprocedural TNF-α and hs-CRP for MACE at 30 days and 1 year post PCI. Time-to-event analyses were performed using the Kaplan-Meier survival curves in patients with various ranges of postprocedural TNF-α and hs-CRP. Results The percentage of anterior myocardial infarction, the peak of Creatine Kinase MB, left ventricular ejection fraction, TNF-α and hs-CRP levels were all significantly different between MACE group and non-MACE group after PCI. Multivariable logistic analysis showed the OR of TNF-α were 1.43 (P=0.006) at 30 days and 1.62 (P=0.002) at 1 year. The OR of hs-CRP were 1.14 (P=0.004) at 30 days and 1.22 (P=0.005) at 1 year. The patients with higher postprocedural TNF-α level had significant lower survival rates at 30-day and at 1-year, compared with the patients with lower postprocedural TNF-α level. The patients with higher postprocedural hs-CRP level had significant lower survival rates at 1-year, compared with the patients with lower hs-CRP level. In higher TNF-α group recurrent myocardial infarction rates at 1-year, revascularization rates at 30-days and at 1-year were higher than those in lower TNF-α group. Compared with those in lower hs-CRP group, recurrent myocardial infarction rates and revascularization rates at 30-days and at 1-year all apparently increased in higher hs-CRP group. Conclusion Postprocedural TNF-α and hs-CRP levels are closely related with short and long-term MACE in first AMI patients treated with successful primary PCI. Related Articles | Metrics

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Clinical Research about Time Window of Puncture and Drainage of Spontaneous Basal Ganglia Hemorrhage through Frontal Approach 2011, 39 (12):  1116-1119 .  Abstract ( 1099 )   PDF (9251KB) ( 2013 )   Objective: To investigate the clinical efficacy of puncture and drainage for the treatment of spontaneous basal ganglia hematoma through a catheter at frontal region at different time window and its impact on brain edema. Methods: A total of 113 patients with spontaneous basal ganglia hematoma were divided into an ultra-early group （n=31, surgery started within 6 hours of the ictus），an early group （n=42, surgery started from 6 hours to 24 hours of the ictus） and a delayed group （n=40, surgery started from >24 hours to 72 hours of the ictus） according to the time window of surgery. The differences in incidence of common complications, the evacuation rate of blood clots, brain edema volume change, and neurological deficit scores （NIHSS） change and long-term follow-up results were compared among the three groups. Results: There were no significant differences in the incidence of common complications, residual hematoma volume, evacuation rate of blood clots and initial brain edema volume among the three groups. 7 days after the ictus perihematomal edema volume was （6.6±4.8） mL and （8.2±5.9） mL in the ultra-early and early groups, respectively. Both volumes were significantly lower than （15.8±15.4） mL in the delayed group （P<0.01）. The neurological deficit score （NIHSS） change was （11.3±6.0） and （12.5±6.3） in the ultra-early and early groups, respectively. Both changes were significantly higher than （6.3±5.4） in the delayed group （P<0.01）. Six months later, the ADL score in ultra-early and early groups was significantly more favorable than the delayed group （P<0.01）. But there were no significant differences between the ultra-early and early groups in these variables. Conclusion: The puncture and drainage through a catheter at frontal region for the treatment of spontaneous basal ganglia hematoma could remove blood clot effectively. Early surgery could alleviate the degree of perihematomal edema, improve neurological outcome and reduce the possibility of rebleeding. Related Articles | Metrics

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Clincle analysis on selection of surgical operation for Clincle stage N0 papillary thyroid carcinoma 2011, 39 (12):  1120-1122 .  Abstract ( 1352 )   PDF (2125KB) ( 2096 )   Abstract Objective Talk about the selection of surgical operation for clincle stage N0(cN0) papillary thyroid carcinoma (PTC), in order to ensure the quality of life patients . Methords 127 cases of PTC with cN0, 71cases with thyroid lobectomy and isthmus lobectomy, 56 cases with the glands resection.113 cases underwent selective lymphadenectomy,44 cases with central lymph node dissection 13 cases with expansion of the Central lymph node dissection, 56 cases with functional neck lymph node dissection. Compared the differences between the various sets of data with chi-square test .Results In 113 cases，36.3%（41/113）of all cases showed implicit lymph nodes metastasis. One case got injury of laryngeal recurrence nerve on account of the tumor invasion. No one got seizures occur with permanent hypocalcemia. Statistics analysis shows that : Ⅱ ~ Ⅳ lymph node metastasis rate was difference between Ⅵ lymph node negative and the positive groups. The difference was significant (P <0.05).The extent of tumor invasion(within cancer capsule, the invasion of the capsule, the invasion of the gland) and the age group （＜45years，≥45years）with Ⅵ lymph node metastasis（P＜0.05）was statistically significant. Body mass index(BMI) and gender with Ⅵ lymph node metastasis（P＜0.05）was not statistically significant. Conlusion The larger extent of tumor invasion and the young age may likely to get lymph nodes metastasis. Patients with cN0 PTC shoude performed central lymph node dissection, further more , to the high-risk patients, we should consider expansion of the central lymph node dissection. Related Articles | Metrics

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Effects of Gekko sulfated polysaccharide on the proliferation and differentiation of hepatic cancer cell line SMMC-7721 2011, 39 (12):  1123-1126 .  Abstract ( 1455 )   PDF (2909KB) ( 2349 )   Abstract Objective: To observe Gepsin on the proliferation and differentiation of hepatic cancer cell line SMMC-7721 .Methods: SMMC-7721 cells were cultured in RPM-1640 medium supplemented with 10% fetal bovine serum at 37℃ in a humidified 5% CO2 incubator. SMMC-7721 cells were seeded in 24-well plates at 1×104 cells/ml .Cells were allowed to grow for one day before being exposed to Gepsin(100μg/ml and 10μg/ml, respectively). SMMC-7721 cells were collected at 30min,then 24, 48, 72, 96, 120, 144h after treatment with Gepsin. Trypan blue stain were used to determine total cell count and viable cell number. SMMC-7721 cells were seeded in 24-well plates at 1×104 cells/ml .Cells were allowed to grow for one day before being exposed to Gepsin(100μg/ml and 10μg/ml, respectively)，At day 7 after treatment with drugs, culture medium was harvested. Then AFP and ALB were detected by rayeroimmunology. At day 7after treatment with drugs, SMMC-7721 cells were examined by light microscopy. The level of VEGF、TGF-β1 supernatant was detected by ELISA. Results: Hepatocarcinoma cell line (SMMC-7721 )were exposed to Gepsin(100μg/ml and 10μg/ml). Gepsin strongly inhibited the proliferation of hepatocarcinoma SMMC-7721 cells. While Gepsin did not have effect on viability of SMMC-7721 cells. Treated with Gepsin, SMMC-7721 cells showed ultrastructural features of differentiation. AFP secretion decreased while ALB secretion increased markedly on Gepsin-treated cells. SMMC-7721 cells showed a round shape under light microscope, while cells changed to spindle shape after exposure to Gepsin. The level of TGF-β1 significantly increased in Gepsin group (100μg/ml) （P<0.01）, the level of VEGF in suramin treated groups significantly increased than control group. Conclusion:⑴Gepsin strongly inhibited the proliferation of hepatocarcinoma SMMC-7721 cells. ⑵ The viability of SMMC-7721 cells was not suppressed by Gepsin. ⑶Gepsin could induce differentiation of SMMC-7721 cells.⑷Gepsin maybe induce the differentiation of SMMC-7721 cells via TGF-β1 ways. Related Articles | Metrics

实验研究

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Apoptosis and expression of Bcl-xl/s in Postconditioning 2011, 39 (12):  1130-1132 .  Abstract ( 905 )   PDF (1038KB) ( 2169 )   Objective：To study the anti-apoptosis and the expression of Bcl-xl/s in postconditioning. Methods: Healthy adult male wistar rats were divided into three groups. (1) Sham group, (2) I/R group, (3) PostC group，TUNEL staining was used to analysis myocardial apoptosis index; Immunohistochemisty was used to detect proteinum.The expression of Bcl-xl mRNA and Bcl-xs mRNA was determined by Real-time PCR. Results:Compared with I/R group,the cardiomyocyte apoptotic index decreased in PostC group, the expression of Bcl-xl protein increased, the expression of Bcl-xl mRNA increased,while the expression of Bcl-xs protein decreased, the expression of Bcl-xs mRNA decreased in PostC group. Conclusion: PostC can significantly decrease apoptosis, Bcl-xl、Bcl-xs mRNA and protein involved in the ischemia-reperfusion-induced myocardial apoptosis. Related Articles | Metrics

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Reporter Vector Construction and Identification of Mice Cbfa1 Gene Promoter 2011, 39 (12):  1133-1135 .  Abstract ( 936 )   PDF (2734KB) ( 2259 )   Abstract Objective: To construct a specific reporter vector to monitor the activity of mouse core binding factor a1 (Cbfa1) gene promoter and to analyze its transcriptional activity. Methods: A DNA segment of Cbfa1 gene promoter (-1000bp-+200bp) was amplified by nested-PCR from mouse genome DNA and correctly connected to promoterless vector PGL3-Basic by restriction enzyme MluI and XhoI. Recombinant plasmid pGL3-Cbfa1 was transiently transfected into osteoblastic cell line MC3T3-E1. The expression of luciferase was detected under the monitor of mouse Cbfa1 gene promoter (-1000bp-+200bp). Results: pGL3-Cbfa1 was the same as the design confirmed by restriction digestion and sequence analysis. High luciferase activity (35 fold greater than that of contro vector pGL3-Basic) was found in the pGL3-Cbfa1 promoter constructs. Conclusion: Mouse cbfa1 gene promoter luciferase reporter vector was successfully constructed and the reporter vetor laid the experiment foundation for screening of anti-osteoporosis medicine. Related Articles | Metrics

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The effects of human growth hormone on proliferation and apoptosis of human breast neoplasm cells 2011, 39 (12):  1141-1143 .  Abstract ( 952 )   PDF (2202KB) ( 2318 )   To study the effects of human growth hormone on pro liberation and apoptosis is of human breast neoplasm cells of human in vivo. Methods : The nude mice randomly were divided into four groups and each group 10 mouse. Nude mice were randomly divided into control group, recombinant human growth hormone (rhGH) group, 5-fluorouracil (5-FU) group and rhGH+ 5-FUgroup after human gastric cancer engraft model of nude ice as successfully established. IP give medicine in each group, and observe the influence of drugs on tumor growth after two weeks. The cell cycle, carryon proliferation antigen, apoptosis index (AI) of human breast cancer cell were investigated by flow cytometry, immunohistochemical detection, and terminal dioxin elatedly transferees-me dieted deoxyurdine troposphere biotin nick end labeling (TUNEL) respectively. Results: Cells in S phase, tumor quality and PCNA of gastric cancer engraft obviously degraded, but A I and G0/G1 phase obviously increased in 5-FU group and rhGH+ 5-FU groups compared with control and rhGH groups (P < 0.05); Compared with 5- FU group, rhGH + 5- FU group of tumor quality, PI and G2M period decline rates significantly better than 5- FU group, G0/G1 phase obviously better than the rate 5- FU group (P < 0.05). Between rhGH group and control group, there was no difference in above indices. Conclusion: Exogenous human growth hormone has no obvious effects on pro liberation and apoptosis of human pancreatic cancer cells in vivo. In vitro r-hGH might promote the sensitivity of chemotherapy of 5-FU to MC'F-7 cells Related Articles | Metrics

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Effect of glucocorticoid inhaled on the expression of SERCA in asthmatic rat’s airway smooth muscle cell 2011, 39 (12):  1144-1146 .  Abstract ( 1490 )   PDF (2082KB) ( 2443 )   Abstract: Objective To investigate the role on budesonide to the expression of SERCA2 in asthmatic rat’s airway smooth muscle cell. Methods According to random method, the rats were divided into normal group, asthmatic group and budesonide intervened group. By inhaled OVA to make asthmatic rat model and primary culture ASMC; Real-time PCR and Western blot were used to detect the expression of SERCA2 in ASMC. Results The expression of SERCA2mRNA and protein in asthmatic group were less than in normal group （P < 0.05）; in budesonide intervened group the expression increased than in asthmatic group （P < 0.05）; but the expression of SERCA2mRNA and protein in budesonide intervened group compared with normal group, the difference was not statistically significant （P > 0.05）. Conclusion The expression of SERCA2 in asthmatic ASMC decreased than in normal group; budesonide could increase the expression of SERCA2mRNA, which could be benefit to promote the calcium reserve in ASMC and reduce airway hyper- responsiveness in asthma. Related Articles | Metrics

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The evaluation of the bone contact of immediate implants after the mandibular non-vascularized bone graft by the examination of the SPECT/CT fusion imaging and histology 2011, 39 (12):  1147-1149 .  Abstract ( 1429 )   PDF (2171KB) ( 2077 )   [Abstract] Objective To evaluate the bone contact of immediate implant after the mandibular non-vascularized bone graft by the examination of the SPECT/CT fusion imaging and histology . Method Six local healthy crossbred dogs were choosed as the experimental animals. The opration of bone rectangular cutting was performed to one side of the mandibular of the experimental animals under general anesthesia. The non-vascularized iliac bone was grafed to repair the jaw defect. 3 dental implants were planted the graft bone and the control side at the same time. All the experimental animal were killed at 12 weeks after the exmanation of the SPECT/CT fusion imaging, and ground section of bone with dental implant was performed to access the histology in the implant-bone interface, bone-implant contact ratio and mineralized bone area of 18 implnts. Result All dental imlant-bone interface healed well. There was no statistical significance in the difference in the radioactivecounting of the region of interest, the CT value, the bone-implant contact ratio and mineralized bone area of immediate dental implant in the mandibular which was the non-vascularized bone graft or the normal mandibular. Conclusion Immediate implant after the mandibular non-vascularized bone graft is high feasible. The ground section of bone with dental implant and the SPECT/CT fusion imaging showed important value in the analysis of the successful ratio of the imlant. Related Articles | Metrics

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Experimental Study on Tirofiban Combined Urokinase Intra-arterial Thrombolysis in the Middle Cerebral Artery Occlusion 2011, 39 (12):  1150-1152 .  Abstract ( 1346 )   PDF (2146KB) ( 2120 )   Abstract :Objective To establish an embolic stroke model in rabbit and explore the efficacy and safety of intra-arterial combined Tirofian and Urokinase thrombolysis on the experimental embolic stroke by comparing diffusion perfusion magnetic resonance imaging before ,during and at the end of the thrombolytic treatment and to assess the subsequent prognostic value of diffusion weighted imaging ( DWI ) and perfusion weighted imaging ( PWI ). Methods The middle cerebral artery occlusion model ( MCAO ) was established in New Zealand rabbit by autologous clot .The MCAO rabbits were randomly divided into intra-arterial tirofiban thrombolysis group ( T group ), urokinase thrombolysis group ( UK group ), combined tirofiban and urokinase thrombolysis group ( T + UK group ) and control group ( C group ). The rabbits of T group were given tirofiban , the rabbits of UK group were given urokinase, the rabbits of T + UK group were given combined tirofiban and urokinase and group C was intra-arterial saline. The recanalized rate , relative-apparent diffusion coefficient ( rADC ) and the neurologic function deficit score were compared among them .Results The repatency rate , rADC and the neurologic function deficit score of T + UK group are better than other groups. Conclution Intra-arterial combined tirofiban and urokinase thrombolysis in the rabbit middle cerebral artery occlusion model （MCAO ） is better than intra-arterial single urokinase, which can promote reperfusion and decrease infarct volume . Related Articles | Metrics

短篇论著

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Using the reverse “Y” skin flap anoplasty treats perineal fistula in infants and discuss the theory evidence. 2011, 39 (12):  1153-1154 .  Abstract ( 1457 )   PDF (2541KB) ( 2396 )   Abstract: Objective: To evaluate clinical significant of the reverse “Y”skin flap anoplasty treats perineal fistula in infants and discuss the pathologic evidence. Methods: A retrospective chart review was performed on 29 patients (male: 25, female: 4) who were treated for the reverse “Y” skin flap anoplasty in our hospital between January 2008 and June 2010. Results: We fellow-up the patients for 6 months till 2 years. Overall, 93.1% of this group experienced good results. The new anus looks good, there is no constipation, prolapsed, soiling and incontinence happened in our group. Conclusions: The reverse “Y”skin flap anoplasty is easy to do, the new anus looks good, and the anal function recovers smoothly. Related Articles | Metrics

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3-aminophenylboronic acid disk synergy test for identication of Class C β-lactamase-producing Escherichia coli 2011, 39 (12):  1156-1158 .  Abstract ( 971 )   PDF (3356KB) ( 2272 )   Objective： To establish and evaluate a new method to detect high producing AmpCβ-lactamase Escherichia coli.Methods:AmpC β-lactamase from 44 strains of cefoxitin-resistant Escherichia coli were detected by 3-aminophenylboronic acid (APB) disk synergy test in comparison with three-dimensional test and polymerase chain reaction (PCR). The results were analysed by statistics analysis method. Result: In 44 strains of cefoxitin-resistant Escherichia coli, AmpC β-lactamase positive rates of three methods were 70.5%，56.8% and 75% respectively.Compared with three-dimensional test, the result showed that the positive coincidence rate、negtive coincidence rate and total coincidence rate were 88.0%，52.6% and 72.7% respectively，there were no differences between APB disk synergy test and three-dimensional test（P>0.05）. Compared with PCR，the result showed that the sensitivity, specificity and the coincidence rate were 90.1%，90.1% and 90.1% respectively, there were no differences between APB disk synergy test and PCR method（P>0.05）. Conclusions:APB disk synergy test is simple,correct,not demanding special equipments. Thus ,it is applicable for routine use in clinical microbiology laboratoryes. Related Articles | Metrics

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Change of Blood Sugar in Hospital elderly patients with hypertension and it’s influence on state of renal function 2011, 39 (12):  1158-1159 .  Abstract ( 992 )   PDF (1454KB) ( 2184 )   Abstract Objective：To investigate Change of Blood Sugar in Hospital elderly patients with hypertension and it’s influence on state of renal function . Methods：Chose elderly patients with hypertension hospitalized for research object, their some index were measured such as BMI, blood lipids, serum creatinine, OGTT,IRT and arterial blood pressure after admission, GADAb were measured in patients with diabetes mellitus. The next day after admission, 2 hours urine was collected to test urine albumin and creatinine concentration and calculation UAER, eGFR was calculated according to Cookroft - Gaut formula; in the First place , all of the patients was grouped on the basis of Whether UAER≥3.5 mg/ mmol to Compare the absolute value of the patients’ eGFR between the two groups patients with the same Blood Sugar state, the proportion of patients with eGFR significantly decrease, and the eGRF absolute value in patients with different blood sugar levels in the same UAER group, The next grouping criteria was whether eGFR acuities 60ml/min. 1.73m2 ,comparing the change of some index including age, sex, BMI, blood pressure and Blood Sugar levels between the two groups. Results：(1)The incidence of abnormal glucose metabolism was 80.70%.（2）There is a higher incidence of renal impairment, showing abnormal UAER and obvious renal insufficiency (53.91% vs21.39% ,respectively) （3）Compared with normal UAER group, there were lower absolute value of eGFR in UAER abnormal group patient regardless of how their blood glucose levels, and the proportion of the obvious decline of renal function was significantly higher. In UAER abnormal group, the levels of eGFR have a statistically difference among patients with different blood glucose levels, eGFR in T2DM group was the lowest in three group. (4) With eGFR> 60 ml / min .1.73 m2 group comparison: age and blood pressure were higher, more common women , with blood glucose levels elevated ,percentage of the obvious decline of renal function increasing in eGFR ≤ 60ml / min .1.73 m2 group, this percentage was the highest in T2DM group . Conclusion：(1) Abnormal glucose metabolism were very common in elderly patients with hypertension hospitalized.(2) The incidence of kidney damage were higher, performance for UAER abnormal or eGFR reduce or both cocurrence. (3) Renal impairment occurrence involves glucose metabolic disorder, elevated blood pressure and the growth of the age. Related Articles | Metrics

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The analytical performance and evaluation of clinical applican about testing method of ADVIA Centaur Ultra-sensitivity TnI 2011, 39 (12):  1160-1161 .  Abstract ( 1586 )   PDF (10197KB) ( 1944 )   [abstract] objective To evaluate the analytical performance of ADVIA Centaur TnI-Ultra testing method, in order to help the clinical diagnosis of AMI . Methods To collect the serum of emergency department patients with chest pain immediately and detect TnI-Ultra. consult 2000 ESC/ACC for AMI diagnostic criteria to diagnos AMI. Calculate the positive rate of immediated detection of patients. according to ROC curves of TnI-Ultra test results in the diagnosis of characteristics with AMI for evaluation. Evaluation of this method function sensitivity (CV = 10%), within- and between run imprecision. Results confirmed immediated positive rate of AMI patients was 34.2%, the area under the ROC curves was 0.956, definite diagnosis threshold value was 0.436 ng/ml, at this threshold value sensitivity was 90.2%, specificity was 87.3%. TnI-Ultra function sensitivity when CV=10% was 0.042 ng/ml, when CV=20% was 0.0195 ng/ml. Low concentration serum (nearly 0.04 ng/ml) within-run CV was 7.9%, between run CV was 8.1%; High concentration serum (0.11 ng/ml) within-run CV was 4.6 %, between run CV was 5.4%. Conclusion ADVIA Centaur TnI-Ultra detection method analysis performance reach "guideline acceptable" (CV=10% or less), but when AMI happened, judgment threshold the provided materials by manufacturers have difference, the application of routine clinical diagnosis still needs further observation and study. Related Articles | Metrics

临床论丛

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The classification of ultrasonic images and the choice of surgeries of chest wall tuberculosis 2011, 39 (12):  1162-1163 .  Abstract ( 1782 )   PDF (1393KB) ( 2072 )   Abstract： Objective To discuss the classification of the chest wall tuberculosis through analysing the ultrasonic images of 76 cases. Methods Collecting the data of 76 cases of chest wall tuberculosis confirmed by surgery and pathology in our hospital and summarizing their ultrasonic characteristics. Results There are 5 types of ultrasonic images according to the pathological changes, typeⅠ:lump shape, 3 cases; typeⅡ: dumbbell shape, 27 cases; type Ⅲ: ruptured shape, 21 cases; type Ⅳ: crab claw shape, 23 cases; type Ⅴ: distant metastases shape, 2 cases. Conclusions The ultrasonic images can clearly indicate the shape and the involved field of chest wall tuberculosis and also their chassification,which has definite clinical significance in the choice of surgeries. Related Articles | Metrics

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Clinical application and research of the treatment of superficial hemangiomas by high-frequency electrocoagulation using fine needle 2011, 39 (12):  1163-1164 .  Abstract ( 1567 )   PDF (8286KB) ( 1924 )   [Abstract] Objective: The study aims at observing the therapeutic efficacy of the superficial hemangiomas, the choice of the indication and the prevention of the adverse effects by the high-frequency electrocoagulation using fine needle.Methods: Between Junly 2007 and June 2008,the 184 cases (197 Lesions)with hemangiomas were treated with high-frequency electrocoagulation using fine needle.Analysising of the clinical date, treatment effects,indications and complication of all cases.Results: The care rate and obvious effects rate of the total lesions were respetively 53.3% and 35%,and the total effective rate was 88.3%.All the patients were followed up for 12-20 months. Among 197 lesions,30 lesions presented pigmentation (15.2％),12 lesions had superficial scar (6.1%),2 lesions had local infection (1%),and no other adverse reactions and accidental injury.Conclusions: It is a safe,efficiency therapeutic technique and easy for operating surgeons to treat superficial hemangiomas by high-frequency electrocoagulation using fine needle.The technique is suitable especially for the treatment of middle or small hemangionmas,in addition which utmostly remains normal anatomic form and function. Related Articles | Metrics

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Management of Post-cardiotomy Cardiogenic Shock with ECMO 2011, 39 (12):  1166-1167 .  Abstract ( 917 )   PDF (1395KB) ( 2224 )   Related Articles | Metrics

短篇与病例报告

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Two cases of dilated cardiomyopathy with acute myocardial infarction 2011, 39 (12):  1183-1184 .  Abstract ( 1352 )   PDF (6895KB) ( 1948 )   Related Articles | Metrics