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15 October 2011, Volume 39 Issue 10 Previous Issue    Next Issue
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论著
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论著

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Construction and Determination of Recombinant Eukaryotic Plasmids of Tudor-SN Protein TSN Domain’s sub-Fragments 2011, 39 (10):  881-883 .  Abstract ( 1405 )   PDF (3109KB) ( 2059 )   Abstract Objective:To construct eukaryotic green fluorescent protein(GFP) expressing recombinant plasmids, pEGFP-C2-Tudor-SN-TSN(Ⅰ~Ⅳ), which contain 4 sub-fragments of human Tudor-SN TSN domain, respectively. Methods: The genes of Tudor-SN-TSN fragments were amplified by PCR from the recombinant pSG5-Tudor-SN-flag plasmid then the EcoRI/Sal I digested fragments were combined with pEGFP-C2 vector. The recombinant expression plasmids pEGFP-C2-TSN(Ⅰ~Ⅳ) were transfected into HeLa cells. The expression of fusion proteins were examined by fluorescent microscopy and Western blot. Results: (1) The sub-fragments of TSN domain can be detected in the products of the restriction double enzyme digestion; (2) The green fluorescent fusion proteins were observed in HeLa cells after transfection; (3) The fusion proteins pEGFP-C2-TSN(Ⅰ~Ⅳ) can be detected from the lysate of transfected HeLa cells by Western blot. Conclusion: (1) The expression plasmids pEGFP-C2-TSN(Ⅰ~Ⅳ) was successfully constructed. (2) These recombinant eukaryotic plasmids can be used for protein detection by binding with anti-GFP antibody for the further study on molecular mechanism in biologic function of Tudor-SN-TSN. Related Articles | Metrics

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Research on genetic polymorphisms of HLA- A31, B40, B58, DRB1*16 in the Hans of Zunyi 2011, 39 (10):  884-886 .  Abstract ( 1486 )   PDF (2086KB) ( 2060 )   Objective :To investigate the frequencies and polymorphism of hemorrhagic fever with renal syndrome (HFRS) associated HLA alleles ,HLA-A31, B40, B58 and DRB1*16 , in the Hans of Zunyi area. Methods: Polymerase chain reaction-sequence specific primers (PCR-SSP) were used to type HLA-A, B, DR genes of 200 unrelated healthy Han individuals in Zunyi area, and then the positive samples of A31, B40, B58 and DRB1*16 were selected and subtyped at high-resolution.. The allelic distribution of these alleles in Zunyi Han populations were compared with other different populations by chi-square test . Results: In HLA-A31, B58, DRB1*16 gene, only one kind of alleles was severally obtained, whereas, in HLA-B40 four kinds of alleles were obtained. Among them, B*4001, B*4002 and B*4006 were common alleles , and B*4063 was one kind of the rare alleles. Comparison of the HLA subtypes frequencies in different populations showed a close relationship of Zunyi Han population with the Han population in Southern China and the distribution of the allele subtypes in Zunyi Han population differed significantly from that of the Japanese. Conclusion: The alleles in HLA-A31、B58、DRB1*16 are relatively single, but the alleles in HLA-B40 have plenty of polymorphism. Their distributions have not only the southern Han’s characteristics but also their own territory feature. Related Articles | Metrics

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Study on the association between EPHX1 polymorphism and warfarin maintenance dose 2011, 39 (10):  887-889 .  Abstract ( 1390 )   PDF (2295KB) ( 2167 )   Objective: To investigate the association between EPHX1 rs4653436（G＞A） polymorphism and inter-individual warfarin maintenance dose. Methods: The genotypes of 217 patients with stable warfarin dose were determined by using of a PCR/DHPLC genotyping assay. The warfarin maintenance doses were compared among patients with different genotypes. Results: In the total of 217 patients, the numbers of GG, GA and AA genotype were 141, 72 and 4, with the frequencies of 64.4%, 32.9% and 1.8%, respectively. The frequencies of G and A allele were 81.6% and 18.4%, respectively. The mean stable doses of patients with GG, GA and AA genotype were 2.84±1.19 mg/d, 2.91±1.09 mg/d and 2.66±1.29 mg/d respectively. There was no significant difference (P=0.483) in mean stable dose among the three genotype groups (P=0.856). Conclusion: EPHX1 rs4653436（G＞A）polymorphism may be not a genetic factor that influence the inter-individual warfarin maintenance dose. Related Articles | Metrics

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Effects of Bacillus Calmette-Guerin on Th1/Th2 balance of peripheral blood mononuclear cells from atopic asthamatics 2011, 39 (10):  890-892 .  Abstract ( 1417 )   PDF (2246KB) ( 2119 )   Abstract Objective:To investigate the effects of Bacillus Calmette-Guerin (BCG) on Th1/Th2 balance of human peripheral blood mononuclear cells (PBMC in asthmatic patients. Methods:PBMC were separated from 20 bronchial asthma patients and 10 non-atopic, age mathed healthy subjects as control. PBMC were cultured with BCG (100μg/ml) for 48h as postive group and PBMC were treated by saline as negative group. The mRNA expression of interleukin-4 (IL-4) and interferon-γ(IFN-γ) were measured by reverse transcription-polymerase chain reaction (RT-PCR) and the peripheral blood Th1/Th2 lymphocytes ratio by using three-color flow cytometry method. Results:The mRNA expression of IL-4 in asthmatics was significantly higher than that in healthy subjects while the mRNA expression of IFN-γwas lower significantly ( all P <0.05). And after BCG stimulation The IL-4mRNA expression decreased but IFN-γmRNA expression increased compared with untreated asthma group ( all P <0.05). The rate of Th1 and blood Th1/Th2 cells ratio were lower in asthma group compared with negative group ( all P <0.05) while the rate of Th2 was significantly higher than that in healthy gorup (P <0.05). After stimulation with BCG the rate of Th1 and Th1/Th2 ratio increased ( all P <0.05) , also the blood Th2 rate decreased compared with untreated asthma group (P <0.05). Conclusion:The Th2 immune response is stronger than Th1 immune response in bronchial asthma patients. BCG can retrieve the excursion of Th1/Th2 both on gene expression level and single-cell level. Related Articles | Metrics

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The Evaluation of Surgical Site Infection Prevention by Short-term Antibiotic Prophylaxis Strategy for Cesarean Section on patients with Pelvic Inflammatory Disease 2011, 39 (10):  893-895 .  Abstract ( 1564 )   PDF (2198KB) ( 2411 )   Abstract Objective: To evaluate effect on prevention maternal/neonatal infectious morbidity by short-term prophylactic antibiotics at cesarean section with pelvic inflammatory disease (PID). Methods: All patients with PID found during cesarean section were collected during December 2009 to February 2011. Part of Perinatal infection were surveyed and compared between short-term (<24 hours, n = 109) and long-term (>48 hours, n = 118) prophylaxis by cefazolin sole or combined with metronidazole at cord clamping. Results: Statistical significant difference had not been shown in incidence of surgical site infections (SSIs), endometritis, puerperal morbidity on pregnant women who accepted cesarean section and found PID whether antibiotics were admitted by short-term or long-term. More than 86% cost reduction was found in prophylactic antimicrobial use by short-term antibiotics prophylaxis (RMB15.80±10.32 to RMB115.87±49.76, P = 0.00). Conclusion: short-term (<24 hours) prophylaxis by cefazolin sole or combined with metronidazole at cord clamping was safe and saved strategy to prevent SSIs of cesarean delivery. Related Articles | Metrics

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The Effect of Estradiol on modulating transcription of different fractions of apoAⅠgene promoter 2011, 39 (10):  896-898 .  Abstract ( 1397 )   PDF (2121KB) ( 2086 )   Objective: to analyze the modulating mechanism of estrogen on different fractions of apo A? gene promoter. Methods: Construct recombinants containing different length DNA fragments of apo AI gene (-41/+397, -256/+397, and -2500/+397) with or without a 7-kb region apo CIII/AIV intergenic region in basic pGL2 vector that containing lueiferase reporter gene. The final recombinants with the internal control vector pRL-null with renilla luciferase reporter gene were transfected to HepG2 cells by the cationic lipid method. After 24h, the cells were treated with estradiol at 10μmol/L concentration; After 48h, cells were collected and lysized. The activities of these luciferase enzymes were measured with Dual-GloTM Luciferase Assay System. Results: In estradiol induced experiments, exception of the recombinant plasmids containing only the basal promoter (-41/+397bp) with or without region of apo CIII/AIV, The all recombinants expression of luciferase enzymes was great increased. There was no different between pGL2/-41AⅠand pGL2/-41AⅠCⅢAⅣ in the presence of estradiol. Conclusion: Promoter area containing -256bp may be the smallest reactive element for estrogen. It can increase transcription activity after stimulating by estrogen. The apoCIII/AIV gene modulating was not affected by estrogen. Related Articles | Metrics

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The expression of Caveolin-1 and Ki67 in cervical cancer and associated research with HPV16/18 infection wangying 2011, 39 (10):  899-902 .  Abstract ( 1203 )   PDF (1391KB) ( 2151 )   Abstract] Objective :The goal of this study was to analyze relationship between the expression levels of Caveolin-1and Ki67 and HPV infection in normal cervical tissue，cervical intraepithelial neoplasia (CIN) and cervical carcinoma，and to analyze its relationship with clinicopathological parameters of cervical cancer-related links to explore the above-mentioned target happened in cervical cancer development，invasion and metastasis，for the diagnosis and treatment of cervical cancer to provide a new and effective target. .Methods : Using immunohistochemical method to detect the expression of Caveolin-1 and Ki67 and HPV in 15 cases of normal cervical tissue，35 cases of CIN tissue, 50 cases of cervical cancer . Results : significant difference for Caveolin-1and ki-67 was existed between normal cervical group and CIN group and cancer group.with increasing of clinical stages and tumor grades ,positive rates have signifucant difference,which were correlated with lymph node metastasis.The positive rate of HPV16/18 was significantly different between cervical cancer, CIN tissue and normal cervical tissue and was not associated with tumor grades ,clinical stages,age group and lymph node metastasis. Conclusion: expression of Caveolin-1 and ki-67 in cervical cancer tissue was correlated with HPV infection.Combined detection of Survivin and Ki-67 may be a new target of cancer diagnosis and treatment and a new way to assess the severity of cervical cancer. Related Articles | Metrics

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The incidence of and risk factors for contrast induced nephropathy after percutaneous coronary intervention 2011, 39 (10):  903-906 .  Abstract ( 1364 )   PDF (2859KB) ( 2339 )   Abstract Objective: To investigate the incidence of and risk factors for contrast induced nephropathy(CIN) after percutaneous coronary intervention(PCI). Methods:A retrospective study was performed on 1010 patients who had underwent PCI in cardiology department of Tianjin chest hospital from January 2008 to December 2010. According to the diagnostic criteria for CIN，the patients were divided into CIN group and non-CIN group, related factors were compared between the two groups. Logistic regression analysis was applied to determine the risk factors for CIN. Results: Among the 1010 patients, CIN occurred in 171 patients and the incidence of CIN was 16.9%. There were significant differences in age, blood pressure, blood glucose, lipid, hemoglobin, red blood cells, hematocrit, creatinine clearance(Ccr), left heart ejection fraction(LVEF), contrast media volume between the two groups. Logistic regression analysis showed that aged≥70 years, myocardial infarction, emergency PCI, diabetes mellitus , hypotention, anemia, Ccr≤60ml/min, contrast media volume>200ml, LVEF≤45% was strongly associated with CIN. Conclusion: Contrast induced nephropathy is a common complication after PCI, more attention should be paid in this complication in clinical. Aged≥70 years, myocardial infarction, emergency PCI, diabetes mellitus , hypotention, anemia, Ccr≤60ml/min, contrast media volume>200ml, LVEF≤45% were risk factors for CIN. Related Articles | Metrics

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The inhibition of celecoxib combined with PDTC on SGC-7901 gastric cancer cells 2011, 39 (10):  907-910 .  Abstract ( 1350 )   PDF (2880KB) ( 2115 )   Abstract Objective:To investigate the effect of inhibition and its mechanism about the selective COX-2 inhibitor celecoxib， and NF-kB inhibitor pyrrolidine dithiocarbamate (PDTC) on human gastric cancer cell line SGC-7901.Methods:SGC-7901 cells were respectively treated in vitro with celecoxib (50、100 μmol/L)and PDTC(50、100 μmol/L) or combination（25/25、50/50 μmol/L）. The methyl thiazolyl tetrazolium (MTT) assays were used to measure the growth inhibition rate of cells. TUNEL assays were used to measure the in situ apoptosis of gastric cancer cells .RT-PCR assays the expression levels of COX-2、NF-kB、caspase-3. Results:The growth of gastric cancer cells were inhibited after treated 72 hours with celecoxib， PDTC or the combination group (P<0.05)，but the combination groups(25/25 μmol/L)didn't do more than the celecoxib (50 μmol/L)groups. The combination groups(50/50 μmol/L) are more effective than single drugs with 100μmol/L(P <0.05). After treated 24 hours by the groups of celecoxib（100μmol/L） ，PDTC （100 μmol/L）or combination groups（50/50 μmol/L），the apoptosis index was significantly different compared with negative control，and the combination groups had higher effect (P <0.05).the expression of COX-2、NF-kB were decreased (P <0.05)and caspase-3 was increased (P <0.05) when the cells had been exposed to celecoxib （100 μmol/L）or PDTC （100μmol/L）by 12 hours，and compared with them，the combination groups (50/50 μmol/L) had obvious changes(P <0.05).Conclusion:Both the celecoxib and PDTC can inhibite the proliferation and promote the apoptosis of gastric cancer cells.the mechanism may associated with inhibiting the expression of NF-kB、COX-2，then promote the expression of caspase-3. Related Articles | Metrics

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The Study on the Systolic and Diastolic Ventricular Syn- chronization in Patients with Coronary Heart Disease Complicating Diabetes Mellitus Evaluated by Doppler Tissue Imaging Technology 2011, 39 (10):  911-913 .  Abstract ( 1416 )   PDF (4756KB) ( 2150 )   ABSTRACT Objective: To detect the ventricular synchronization in coronary heart disease patients with no heart failure sympathion and the effection of the combination with diabetes mellitus(DM) by Dopler tissue velocity imaging (TVI) technology. Methods: Selected127 cases of patients with coronary heart disease as the study group which was divided into groups with diabetes with coronary heart disease (CHD-DM group) and non-diabetic coronary heart disease (CHD-NG group). ⑴To measure and calculate the interventricular septum(IVS) and left ventricular posterior wall(LVPW) thickness, the left ventricular end systolic and diastolic diameter (LVEDs and LVEDd) and the volume (LVEVs and LVEVd), left ventricular ejection fraction(LVEF) . ⑵To measure time to peak velocity at systolic and early diastolic phase (Ts and Te) in the base and interlude altogether 14 segments of left and right ventricular in all subjects. To calculate and compare the average time of Ts and Te in left ventricular 12 segments (Ts-12-LV and Te-12-LV), the 12 segments standard deviation of Ts (Ts-12-SD) ,the 12 segmental maximal Ts and Te difference (Ts-12-diff and Te-12-diff) and the average time of Ts and Te in right ventricular 2 segments(Ts-2-RV and Te-2-RV). Results: 1. The Ts in 11 segments of left ventricular, Ts-12-LV, Ts-2-RV, Ts-2-RV and Ts-12-diff were failed to demonstrate any statistically significant differences between CHD-DM group and CHD-NG group (p＞0.05). by the Ts-12-SD＞33 ms as the standard , CHD-DM group who achieve this standard were 17 cases, 11 cases of the control group. To Ts-12-diff＞100 ms as the standard, the ratio of the two groups were 50%(34/68) and 28.8%(17/59), p＜0.01. the Te-12-diff were also failed to demonstrate any statistically significant differences between the two group(p＞0.05)，but under the standard of Te-12-diff＞100 ms, the two groups accounted for 47.1% and 25.4% (p＜0.01). 2.In the CHD-DM group Vs and Ve in most segments of left and right ventricular were lower than that of CHD-NG group, especially in group of CHD-DM and CRBBB(p＜0.05 or p＜0.01). Conclusion: The Ts-12-diff and Te-12-diff in patients with coronary heart disease who didn’t have symptoms of heart failure and unnormal QRS duration were significantly longer, especially in patients who had coronary heart disease with diabetes mellitus, suggesting that the systolic and diastolic synchronization was reduce in patients with coronary heart disease, diabetes can increase it. Related Articles | Metrics

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Electrophysiological studies of phrenic nerve in patients with acute exacerbation of chronic obstructive pulmonary disease 2011, 39 (10):  914-917 .  Abstract ( 1247 )   PDF (2832KB) ( 2165 )   Objective: To investigate the characteristics of phrenic nerve motor conduction (PNC) and motor evoked potentials of diaphragm elicited through magnetic stimulation (dMEP) in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) who experienced acute respiratory failure. Methods: PNC and dMEP were performed in 20 AECOPD patients who experienced acute respiratory failure enough to require mechanical ventilation with a Keypoint 4 electromyography device and a Magpro Compact magnetic stimulator. 20 healthy subjects were chosen as a control group. Results: The latency of PNC in AECOPD group was longer than that of the control group (P<0.01); the common logarithm of the amplitude of PNC was lower in AECOPD group than that of the control group (P<0.01); the latencies of cervical and cortical dMEP, CMCT in AECOPD group were longer than those of the control group (P<0.01 for each); the common logarithm of the amplitude of cervical dMEP in AECOPD group was decreased compared with that of the control group (P<0.01); it showed no statistical difference between two groups in the common logarithm of the amplitude of cortical dMEP (P>0.05).13 patients in AECOPD group were reexamined after weaning, the differences between two groups were the same as before weaning in latency and the common logarithm of the amplitude of PNC, the latency and the common logarithm of the amplitude of cervical dMEP and the common logarithm of the amplitude of cortical dMEP; there were no statistical differences between two groups in the latencies of cortical dMEP and CMCT (P>0.05 for each). Conclusion: There may exist dysfunction of respiratory central motor conduction, phrenic nerve and (or) diaphragm in AECOPD patients, the combined use of PNC and dMEP may help evaluate the function of phrenic nerve, diaphragm and respiratory central motor conduction in AECOPD patients. Related Articles | Metrics

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A study on the measurement of DKK-1 in the serum of ankylosing spondylitis patients 凯 张2, 2011, 39 (10):  918-920 .  Abstract ( 1002 )   PDF (2121KB) ( 2215 )   Objective This study was undertaken to explore the expression of Dkk-1 and its potential role in patients with ankylosing spondylitis (AS). Methods Serum Dkk-1 levels were measured by sandwich enzyme linked immunosorbent assay (ELISA). Two groups of AS subjects were tested. The first group consisted of patients who have not been treated with biological agents. In the second group, patients had been receiving anti- TNFα treatment more than 12 weeks. These were compared to serum samples from 8 patients with rheumatoid arthritis (RA) and 20 healthy subjects. Disease activity assessment was performed using the Bath AS Disease Activity Index (BASDAI) in patients with AS, the Disease Activity Score in 28 joints (DAS28) in patients with RA. All patients with AS and RA had active disease. Markers of inflammation (erythrocyte sedimentation rate [ESR] and C-reactive protein [CRP] levels) were measured in all patients. Results Serum Dkk-1 levels were significantly decreased in AS patients who have not been treated with biological agents as compared with patients with RA (p=0.037), and normal subjects (p=0.012). The BASDAI，ESR and CRP in patients with AS did not correlate with serum Dkk-1 levels. Patients with AS receiving anti-TNFα treatment had significantly higher serum Dkk-1 levels compared with patients with AS not receiving this treatment (p=0.000). Conclusion Our results indicate that the levels of serum Dkk-1 in patients with AS, a prototype bone-forming disease, is decrease. No association between circulating Dkk-1 levels and disease activity or markers of inflammation (ESR and CRP) in any AS patient group. Furthermore, TNFα could induce the expression of Dkk-1 in patients with AS. Related Articles | Metrics

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The Correlation of Lp（a） Levels to the Extent, Severity of Coronary Artery Lesion in Coronary Artery Disease Patients with 2 Type Diabetes Mellitus YU Xiang-Dong YU Xiang-Dong YU Xiang-Dong 2 2011, 39 (10):  921-923 .  Abstract ( 1368 )   PDF (2155KB) ( 2251 )   Abstract Objective: To observe the correlation of lipoprotein(a)[Lp（a）] levels to the extent , severity of coronary artery lesion in coronary artery disease (CAD) patients with 2 type diabetes mellitus. Methods: A total of 163 CAD patients angiographically documented were divided into two groups including moderate stenosis group and severe stenosis group according to severity of coronary artery lesion, divided into three groups including monovessel disease group, bivessel disease group and multivessel disease group according to the extent of coronary artery lesion. 54 subjectswithout CAD as control group. Serum lipid levels （total cholesterol: TC; triglycerides:TG; high-density lipoprotein-cholesterol:HDL-C; low-density lipoprotein -cholesterol:LDL-C; Apolipoprotein A-I : ApoA-I; Apolipoprotein:ApoB） were measured. Results: The Lp（a）levels were significantly higher in moderate stenosis group and severe stenosis group as compared to the controls, and there was no difference between moderate stenosis group and severe stenosis group. The Lp （a） levels were significantly higher in monovessel disease group, bivessel disease group and multivessel disease group as compared to the controls, and higher in multivessel disease group as compared to monovessel disease group and bivessel disease group. No difference of TC, TG, HDL-C, LDL-C, ApoA-I, ApoB levels among moderate stenosis group , severe stenosis group and control group was found , and there was no difference among monovessel disease group, bivessel disease group, multivessel disease group and control group. Logistic regression analysis indicated that Lp（a）levels were independently associated with the extent, severity of coronary artery lesion in Type 2 diabetic patients with CAD. Conclusion: Our data suggest that Lp（a）levels may be helpful in determining the extent, severity of coronary artery lesion in Type 2 diabetic patients with CAD. Related Articles | Metrics

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Clinical study of recombinant human brain natriuretic peptide in patients with acute myocardial infarction complicated with acute left heart failure 2011, 39 (10):  927-929 .  Abstract ( 1355 )   PDF (2157KB) ( 2278 )   Objective： To observe the effect and safety of recombinant human brain natriuretic peptide(rhBNP) in treating patients with acute myocardial infarction complicated acute left heart failure.Method:. Based on different treatment models, fifty-two patients with acute myocardial infarction complicated with acute left heart failure were divided into two groups, one is the recombinant human brain natriuretic peptide(rhBNP) group for 30 cases(treatment group )and the other is nitroglycerin group for 24 cases (control group)． All of these patients’ vital signs,clinic status and laboratory data （BP、UV 、HR、BNP 、kidney function and the day of in hospital） were observed at the day before and after treatment. Results:Compared with the control group，the treatment group showed a more improve of HR 、UV and BNP after treatment, the differences were statistical significance(P<0.001). The level of BP , kidney function before and after treatment had no statistical difference(P>0.001).Compared with the control group, the treatment group showed a more improve of clinical effect. Conclusions：The rhBNP can improve heart function of dilatation and contraction and clinical condition with acute myocardial infarction complicated with acute left heart failure obviously，and has reliable and safely effect．The rhBNP is superior to nitroglycerin in treating patients with acute myocardial infarction complicated with acute left heart failure. Related Articles | Metrics

实验研究

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The effects of p38 inhibitor on the immune cells in irradiated mice 2011, 39 (10):  933-935 .  Abstract ( 872 )   PDF (2229KB) ( 2068 )   Objective To observe the effects of p38 inhibitor SB203580 (SB) on the immune cells in the radiated mice. Methods C57BL / 6 mice were divided into control group, irradiated group, SB group. The control group received sham irradiation, and the rest accepted 6 Gy total body irradiation. Mice in SB group were intraperitoneally injected 15mg/kg at 1,3,5,7,9 day after irradiation. After mice exposed to 6Gy irradiation 10 day, the peripheral bloods and bone marrow cells were collected. The immune cells phenotypes in peripheral bloods were analyzed by flow cytometry, ROS (reactive oxygen species) level of bone marrow cells were detected by kits. Results Compared to control group，the proportion of CD8, B220 and WBC in the irradiated mice significantly decreased, ROS level of bone marrow cells increased (p <0.01). Compared to irradiation group, the CD8 and WBC in SB treated group increased, ROS level in bone marrow cells of SB treated group decreased (p <0.05). Conclusion The results indicate that p38 inhibitor can protect the immune cells from radiation injury by lowing the ROS level and it might used as a therapeutic method for the radiation injury . Related Articles | Metrics

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The effect of dl-3-n-Butylphthalide on Morris water maze and long term potentiaion in vascular dementia rats 2011, 39 (10):  936-938 .  Abstract ( 1427 )   PDF (2791KB) ( 2222 )   Abstract: Objective Vascular dementia (VD) is one of the most common forms of dementia with a progressive decline in memory and cognitive function. In the present study, we investigated the effect of dl-3-n-Butylphthalide (dl-NBP) on learning and memory impairment induced by chronic cerebral hypoperfusion in rats. Methods Male Wistar rats fed with high fat were orally administered 40 mg/kg dl-NBP daily for 30days after bilateral permanent occlusion of the common carotid arteries. Morris water maze (MWM）test was employed to assess spatial memory and learning. The LTP induced by high frequency stimulation (HFS) at CA3-CA1 synapses of the hippocampus was recorded for measuring the slope of field excitatory postsynaptic potential (fEPSP). Results The LTP in the CA1 region of the hippocampus was enhanced more obviously in dl-NBP treatment group than that in VD group. The average amplitude of LTP was significantly increased by 120.5± 7.3% in dl-NBP treated group and 108.4±16.9% in VD group compared to those of basal condition, respectively. There was statistical significance between the two groups (P<0.01). The MWM test revealed that there were significant differences between two groups in the acquisition phase and in the retention phase, implying a considerable improvement of spatial memory in VD+dl-NBP group compared with that of VD group. Conclusion The data suggested that dl-NBP had beneficial effect on the spatial learning and memory of VD rats, also suggested the therapeutic potential for cognitive deficits of dementia induced by chronic cerebral hypoperfusion. Related Articles | Metrics

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Effect of KangNaoYe on the AA contents in serum and brain tissue in rats during Cerebral ischemia-reperfusion 2011, 39 (10):  939-941 .  Abstract ( 787 )   PDF (2252KB) ( 2029 )   Abstract: Objective:To observe the effect of KangNaoYe on the AA contents in serum and brain tissue in rats during cerebral ischemia-reperfusion in order to investigate the mechanism of action of KangNaoYe.Methods: Rats were randomly divided into 6 groups,such us sham-operation grop, ischemia-reperfusion grop, KangNaoYe grops with different dose, Edaravone grop. The models of cerebral ischemia-reperfusion were established in rats by methods of MCAO（middle cerebral artery occlusion）.The animals were killed to the deadline of the schedule.The contents changes of Asp,Glu,Gly,γ-GABA in serum and brain tissue were were detected by reversed-phase High Performance Liquid Chromatography with pre-column derivatization respectively. Results: Compared with ischemia-reperfusion grop, Gly,γ-GABA activities were increased significantly , Asp,Glu content were decreased significantly in KangNaoYe groups(P<0.05~0.01). Conclusion: KangNaoYe shows protective effects on cerebral ischemia-reperfusion. The mechanism is that KangNaoYe can mediate the level of AA in blood serum and brain tissue. Related Articles | Metrics

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The Effects of Exendin-4 on Glucose and Lipid Metabolism in Diabetic Rats 2011, 39 (10):  942-944 .  Abstract ( 1513 )   PDF (2107KB) ( 2113 )   Objective: To investigate the effects of Exendin-4 on glucose and lipid metabolism and gastric emptying in type 2 diabetic(T2DM) rats. Methods: Rat model of T2DM was established by high fat-sugar diet fed and low-dose streptozotocin-treated. SD rats were divided randomly into normal control group(NC), normal+ Exendin-4 group(NE), diabetes control group(DC) and diabetes+exendin-4 group. NE and DE rats were given Exendin-4（2ug/kg） twice daily for 50 days. Half time of gastric emptying and emptying rate were evaluated by SPECT. The serum level of fasting blood glucose (FBG), glycosylated serum protein (GSP), free fatty acids (FFA), total cholesterol (TC), triglyceride(TG) tissue were measured by kit. Results: Compared with NC group, DC rats has higher FBG, FFA, TC, GSP, TG and emptying rate(P<0.05), but half time of gastric emptying decreased significantly(P<0.05). Compared with DC group, FBG, GSP, FFA and emptying rate of DE rats were reduced(P<0.05), but has more half time of gastric emptying(P<0.05). The results of Multivariate stepwise regression analysis showed that FBG related to half time of gastric emptying. Conclusion: Type 2 diabetic rats has faster gastric motility, higher blood glucose and lipid. Exendin-4 might improve the disorders of blood glucose, free fatty acids and gastric emptying in diabetic rats. Key words Exendin-4; diabetes mellitus, type 2; diabetes complications; fatty acids, nonesterified; blood glucose; gastric emptying; models, animal Related Articles | Metrics

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The research on the expression of ICAM-1 in the brain of rats with hyperhomocysteinemia and after the intervention of B Vitamins , 2011, 39 (10):  945-947 .  Abstract ( 1499 )   PDF (3740KB) ( 2053 )   Objective To observe the expression of ICAM-1 in the brain of rats with Hhcy or after the intervention of folic acid, Vitamins B12 and Vitamins B6. Methods 40 healthy male Sprague-Dawley rats were divided into 4 groups as:normal group, Hhcy group,treat group, prevent group by the way of different foods and different interventions.The plasma total homocysteine (tHcy) levels of rats were assayed by high-performance liquid chromatography method(HPLC) in different time; At the end of 12th week ,the expression of intercellular adhesion molecule-1 (ICAM-1) were detected by immunohistochemical method in the cerebral vessels of rats. Results At the end of 4th week, the Hhcy model were induced successfully; At the end of 12th week ,there were no statistical difference between the treat group,prevent group and normal group; there were significantly statistical differences between the number of masculine cerebral vessels in the 4 groups(P＜0.05), the sequence is Hhcy group（3.23±0.58）＞treat group（1.54±0.36）＞prevent group（0.98±0.23）＞normal group（0.35±0.31）. Conclusions The expression of ICAM-1 were increased in the cerebral vessels of rats with Hhcy.The intervention of B Vitamins could decrease. Related Articles | Metrics

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Effects of sodium aescinate on p38 mitogen-activated protein kinase signal transduction pathway in lung injury induced by intestinal ischemia reperfusion 2011, 39 (10):  948-950 .  Abstract ( 906 )   PDF (11437KB) ( 1903 )   Abstract Objective: To explore the effects of sodium aescinate(SA) on p38 mitogen-activated protein kinase(p38MAPK) signal transduction pathway in lung injury induced by intestinal ischemia reperfusion. Methods: 24 SD rats were randomly divided into three groups:sham group,IR group,treatment group. In sham group ,superior mesenteric artery was separated only; Models of intestinal IR injury were estabished with clamping of the superior mesenteric artery for 1h and then clamping was relieved for 1h. In treatment group, SA(0.9μg/g) was injected through tail vein before ischemia, before reperfusion and after reperfusion 30min respectively. Pulmonary surfactant(PS), lung wet/dry weight ratio, xanthine oxidase(XOD), malondialdehyde(MDA) , myeloperoxidase (MPO) in plasma and lung tissue were measured, as well as the expression levels of p38MAPK and Bax proteins in lung tissue were examined using immunohistochemical method. Results: (1) In IR group, PS were significently decreased than that in sham group(P<0.01), and while lung wet/dry weight ratio were significantly increased than that in sham group(P<0.01), The above-mentioned indexes of treatment group were obviously ameliorated in comparison with IR group(P<0.01).(2) The activity of ,XOD,MPO and the content of MDA in in plasma and lung tissue were significently increased in IR group than that in sham group (P<0.01). The above-mentioned indexes of treatment group were obviously lower in comparison with IR group(P<0.01). (3)The protein expression level of p38MAPK and Bax were significantly increased in IR group than that in sham group and treatment group(P<0.01).(4)The correlation analysis indicated that MDA,MPO and the expression levels of Bax proteins in lung tissue were positively correlated with the expression levels of p38MAPK proteins(P<0.01). Conclusion: Sodium aescinate can prevent the lung injury induced by intestinal ischemia reperfusion(IR) ,such protective effect may be related to the adjustment of the p38MAPK signal transduction pathway by inhibiting the generation of oxygen free radicals and activation of neutrophils and then prevent apoptosis. Related Articles | Metrics

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The Significance of HIF-1αexpression and Apoptosis in the Kidney of Early- -stage diabetic nephropathy Rats 2011, 39 (10):  951-954 .  Abstract ( 1312 )   PDF (5028KB) ( 2121 )   [Abstract] Objective To explore the significance of HIF-1а expression and the apoptosis in the renal tissue of early-stage diabetic rats. Methodes The Sprague- -Dalwley(SD) rats were divided into the diabetic and control groups.Diabetic group was treated by streptozotocin(STZ). The expression of HIF-1а in renal tissue was detected by immunohisochemistry and Western Blot at 2, 4, 8weeks; The apoptosis of renal cell was measured by TUNEL assay at the same time. The level of HIF-1а expression and the renal index of apoptosis (AI) were quantified by computer image analysis.The relationship between HIF-1а and the renal AI, renal/body weight, urinary protein excretion was analyzed. Results HIF-1а staining in the renal tubular and interstitial cells of Early-stage Diabetic Rats was significantly increased at 2,4 and 8 weeks, as compared with control group; The renal tubular and interstitial cells AI was higher than that of control group at the same time points.The level of HIF-1а expression was positively correlated with KI and urine protein excretion, respectively, So was the renal AI. It was found that there was a strong positive correlation between the level of HIF-1а and the renal AI. Conclusion The upregulated expression of HIF-1а and the increased apoptosis of renal cells in early-stage DN rats could contribute to the development of DN. Their interaction may promote the renal injury of diabetes mellitus. Related Articles | Metrics

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Organic selenium lowering blood glucose and blood lipid in rats Runhe FENG 2011, 39 (10):  955-956 .  Abstract ( 1637 )   PDF (1439KB) ( 2133 )   ABSTRACT Objective: To investigate the organic selenium on the hypoglycemic effect of high glucose in mice and hyperlipidemic mice with lipid-lowering effect. Methods: (1) 30 mice were randomly divided into 3 groups, control group, pathological model group and treatment group, treatment group received organic selenium protein powder, the control group received the same volume of water, taken after the last administration of blood to determine fasting blood glucose levels. (2) 30 mice were randomly divided into 3 groups, control group, high fat model group and treatment group, treatment group received organic selenium protein powder, fat control group and model group were given equal volume of water. After the last administration 2h, except control group, model group and treatment group mice were injected 75% egg yolk; 20h after the blood, serum TC, HDL, LDL, TG content. Results: (1) to prepare a successful model of diabetes, pathological model group and treatment group blood glucose levels were significantly higher; blood sugar treatment group was significantly lower than the pathological model group. (2) hyperlipidemia model group TC, LDL and TG were significantly higher than the treatment group and control group; treatment of the serum TC, LDL, TG levels were significantly lower than model group, treatment group, serum TC, LDL, TG levels were significantly higher or significantly. Conclusion: Organic selenium protein powder significantly reduced serum lipids and the role of blood glucose in diabetic mice. Related Articles | Metrics

短篇论著

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Role of food intolerance in irritable bowel syndrome with diarrhea 2011, 39 (10):  957-958 .  Abstract ( 1329 )   PDF (1462KB) ( 2179 )   【Abstract】　Objective: To investigate the etiopathological role and mechanism of food intolerance in the diseases of irritable bowel syndrome with diarrhea ( IBS-D) in order to provide a diagnosis bases for clinical diagnosis and treatment of the diseases.Methods: The specific IgG antibodies of 14 kind of different food antigens in serum were detected by the enzyme linked immunosorbant assay (ELISA) in seventy seven cases of patients in IBS-D with Rome III and twenty six normal controls.Results: The positive rate of serum food-specific IgG antibodies was 50.6 percent in IBS-D and 15.4 percent in normal controls . there was statistically difference between the two groups (P< 0.01). there is also statistically difference between the two groups in IgG antibody levels (Z =- 3.220, P = 0.001). The positive rate of 14 kinds of serum food-specific IgG antibodies in IBS-D were and followed:egg white/yolk （32.5%）＞ tomato（14.3%）＞crab （9.1%）＞milk and soybean（6.5%）＞mushroom （3.9%）＞corn（2.6%）＞rice、 wheat、codfish and chicken（1.3%）.Only low increased levels of specific IgG to egg white/yolk、milk and tomato in normal controls were found and the specific IgG to other foods were negative. Conclusion:The abnormal humoral immune reactionsmediated by food intolerance played an important role in the immunopathogenesis of IBS-D. Related Articles | Metrics

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Identification of three new mutations (A165D, Q301X and R413G) of phenylalanine hydroxylase gene 2011, 39 (10):  960-961 .  Abstract ( 1567 )   PDF (3161KB) ( 2316 )   【Abstract】Objective： To identify the three new mutations(A165D,Q301X and R413G) of phenylalanine hydroxylase gene by detection of the normal population. Methods： We designed the mismatch primers and extracted the genomic DNA by slating-out method, detected the patients with the mutations and 100 normal controls by using the polymerase chain reaction and the restriction enzyme digestion. Compared the results by agarose gel electrophoresis. Results： The control group was not detected the three mutant alleles except the patients. Conclusion： A165D, Q301X and R413G were three new mutations of phenylalanine hydroxylase gene, which lay the foundation for further investigation of the impact of enzymatic activity. Related Articles | Metrics

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Clinical analysis of patients with delirium in the emergency room Xiang-Hong LIU 2011, 39 (10):  962-963 .  Abstract ( 1380 )   PDF (1398KB) ( 2072 )   Objective To explore the etiology and clinical features of delirium patients in the emergency room.Methods To retrospective analysis the clinical data of 75 delirium patients who were conformed to DSM-Ⅳ delirium diagnosis standard.Results The first cause of non-elderly group was poisoned, while the first cause of the older group was a combination of factors. The hyperactive form of delirium is more common among non-elderly group,while the hypoactive form is more common among older group .Conclusion Delirium causes many clinical manifestations of the complex. Non-senile first need to consider poisoning, intracranial infections, senile delirium shall first consider a combination of factors, and they must attach great importance to the diagnosis of hypoactive delirium. Related Articles | Metrics

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Surveying The Clinical Curative Effect of Sodium Ferulate On Diabetic Nephropathy 2011, 39 (10):  965-966 .  Abstract ( 882 )   PDF (1426KB) ( 2217 )   [Abstract]Objective：Survey the clinical curative effect of Sodium Ferulate on Diabetic Nephropathy。Methord：Eighty-two Diabetic Nephropathy patients，divided into Conventional Therapy Group and Sodium Ferulate Therapy Group randomly，determine Urine Albumn Excretory Rate(UAER)、Blood Urea Nitrogen(BUN)、Serum Creatinine(SCr)、 Free Blood Glucose(FBG) and Mean Artery Pressure (MAP)of all patients，and take the data of pretherapy and post-treatment by comparison。Result：Maintenance single therapeutic regimen for four weeks，in Sodium Ferulate Therapy Group，UAER、SCr、BUN、MAP are significantly cut down（P<0.05，P<0.01），compared with the prethrepy data。However，there is no significant diference between the pretherapy data and post-treatment data。 Conclude：Sodium Ferulate therapy on Diabetic Nephropathy，the value of UAER、BUR、SCr and MAP of patients decrease。Conceivable mechanism is that Sodium Ferulate could inhibit the secretion of endothelin，or degrade the process of binding of endothelin and its receptor。 Related Articles | Metrics

临床论丛

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Operative treatment of the terrible triad of the elbow 2011, 39 (10):  969-970 .  Abstract ( 1350 )   PDF (7019KB) ( 1975 )   Objective : To retrospectively reviewed the operative therapy of the terrible triad of the elbow. Methods: From January 2009 to September 2010, 7 cases of terrible triad of the elbow were treated, with an elbow dislocation and an associated fracture of both the radial head and the coronoid process. There were 6 males and 1 females with the age of 24-36 years. The injury was caused by falling from a height in 5 cases, traffi c accidents in 1 case, and tumbling in 1 case. The coronoid process fractures of the patients were 1 cases of type I, 6 cases of type II according to Regan-Morrey classification. The radial head fractures of the patients were 1 case of type I, 3 cases of type II and 2 case of type III according to Mason classifi cation, and 1 case companied with radial neck fracture.All the patients were treated with operation. coronoid process fracture,. radial head fracture and medial collateral ligaments were repaired in operation. Postoperatively, plaster fixation were given. The function of the elbows were evaluated with the criteria of the Mayo score system. Results: The wounds healed by first intention. The patients were followed up 5 to 15 mouths (mean 11.2 months). According to the criteriaof the Mayo score, the results were excellent in 3 cases, good in 2 cases, poor in 1 case, bad in 1 case. Ossification occurred in 1 patient. Conclusion: The terrible triad of the elbow can lead to serious elbow instability and should be treated with operation to restore the bone and soft structures, early exercise can avoid immobilization and recover the articular function. Related Articles | Metrics

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The changes of volumetric capnography in patients with Idiopathic pulmonary fibrosis 2011, 39 (10):  972-973 .  Abstract ( 1397 )   PDF (1425KB) ( 2205 )   Abstract Objective:To investigate the changes of volumetric capnography(VCap) in patients with Idiopathic pulmonary fibrosis. Methods: Ventilatory function, diffusing capacity and Vcap were determined in 35 patients with idiopathic pulmonary fibrosis and 38 healthy controls.SPSS11.0 were used for statistical analysis. Results： IPF group showed restrictive ventilation and reduced diffusion capacity; VC, DLCO were significantly lower than the controls( p <0.01). VCap parameters of the slope than in normal group Ⅱ no difference, p> 0.05; VT was significantly lower than the normal group, p <0.01; VDB / VT and the slope was significantly higher than in stage Ⅲ, p <0.01; VDB slightly higher than normal, p < 0.05. FEV1% and VCap parameters showed no significant correlation; VC, DLCO were significantly positively correlated with the VT, with the VDB / VT, Ⅲ of the slope was significantly negative correlation, p <0.01, with VBD and Ⅱ was no correlation of the slope, p> 0.05. Conclusions：Patients with idiopathic pulmonary fibrosis, changes in volumetric capnography showed: VT decreased; VDB increases; VDB / VT increased; Ⅱ on the slope of the normal; Ⅲ disappearance of the platform, the slope increased significantly. Related Articles | Metrics

综述与讲座

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Azole Antifungal agents with Torsades de pointes adcc102 ,Zhen-Hua NIE 2011, 39 (10):  982-984 .  Abstract ( 949 )   PDF (2029KB) ( 2102 )   [Abstract] Torsades de pointes (TdP) is a malignant arrhythmia, mostly secondary to the use of certain drugs. Recent studies have suggested that it is important antifungal agents can induce TdP. The mechanism including the inhibition of myocardial hERG potassium channel of cardiac repolarization, as well as the inhibition of cytochrome P450 and so on. It also closely related with the individual factors. Related Articles | Metrics