Table of Content

15 September 2010, Volume 38 Issue 9 Previous Issue    Next Issue
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实验研究
论著
临床论丛
实验研究
论著
实验研究
论著
临床论丛
短篇论著
实验研究
短篇论著
论著
短篇论著
实验研究
论著
临床论丛
短篇与病例报告
临床论丛
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实验研究

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An experimental study of percutaneous nucleoplasty of the cervical intervertebral disc 2010, 38 (9):  0-793 .  Abstract ( 1117 )   PDF (1265KB) ( 2160 )   Abstract Objective: To determine the histologic changes of the cervical intervertebral discs and surrounding tissues after nucleoplasty. Methods：The experimental study was performed in 10 sheep, and each sheep was treated with nucleoplasty in C4-5 and C5-6. Chronic histologic changes were observed by light microscopy at 0，2，4 weeks after cervical nucleoplasty. Results：Two sheep were euthanized immediately after treatment, and the oval-shaped cavities were observed on the macroscopic view in the nucleus pulposus. The cavities measured between 1.5 mm to 2.3mm in long axis and between 0.4mm to 0.7mm in short. 2 weeks after nuleoplasty, the cavities were shortened. 4 weeks after nucleoplasty, the cavities was replaced by nucleus pulposus and fibrous tissues. Condusion: Nucleoplasty did not result in thermal damage or other significant damaging effects to the adjacent annulus and end plate, the cavities produced by nucleoplasty were rehabilitated finally. Nucleoplasty is a safe procedure to cervical spine. Related Articles | Metrics

论著

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Effect of Transforming Growth Factor-β1 on the Proliferation and smad3、 7 protein expression in JEG-3 cells 2010, 38 (9):  0-793 .  Abstract ( 1347 )   PDF (1085KB) ( 1879 )   【ABSTRACT】Objective：To inwestigate the effect of TGF-β1 on the Proliferation and smad3、7 protein expression in JEG-3 cells.Methods： the human choriocarcinoma cell line JEG-3 were cultured in vitro.After exposed to TGF-β1, the proliferation of JEG-3 cells was measured by MTT assay. The espression of smad3、7 protein was measured by Western blotting .Conclusion：The external TGF-β1 can stimulate the proliferation of JEG-3 choriocarcinoma cells, and showing excellent concentration and timing effect. Smad7 protein response missing, Smad3 protein expression in abnormally high levels, suggesting that choriocarcinoma JEG-3 cells to escape TGF-β1-mediated -mediated growth inhibition may be due to the downstream signaling pathway TGF-β1/Smads exception occurs. Related Articles | Metrics

临床论丛

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The pathological study on sudden death of anaphylaxis 2010, 38 (9):  0-793 .  Abstract ( 1482 )   PDF (500KB) ( 2513 )   Abstract：Objective: study on the characteristics of died-cases with allergic shook in Autopsy and their pathological diagnosis criteris. Methods: Review study 10 autopsy cases in our department between 2002 to 2010.Results: 10 cases were between 18 to 88 years old .The most common reason resulting in anaphylaxis was Cephalosporin. The allergic cases usually happen at the private clinics and the base hospitals .The edema of throat of 6cases was found in Autopsy, 9cases were acute pulmonary edema, there were 7cases with mast cell releasing granules .Conclusion: sudden death of anaphylaxis should synchronously consider clinical symptoms, history, observation in autopsy and most cell releasing granules. Related Articles | Metrics

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Expression of connective tissue growth factor in the renal tissue of rats with unilateral renal venous deligation. 2010, 38 (9):  0-793 .  Abstract ( 1243 )   PDF (1947KB) ( 1812 )   Abstract Objective: To investigate the expression of connective tissue growth factor(CTGF) in rat unilateral renal venous deligation animal model. Methods: Male Wistar rats were subjected to left renal vein ligature and sham operation, then killed at 5, 10, 15, 20 or 25 days after renal vein ligature or sham operation (n=5 at each time point). The morphological changes of tubulointerstitial fibrosis were observed by light microscopy with HE, PASM and Masson staining and the expression of CK, CTGF and α-SMA were detected in the renal tissues by immunohistochemistry. With computer aided video system, all results were run by semiquantitative analysis, t-test, mono-factor ANOVA and dependablity analysis test. Results: The extent of tubulointerstitial fibrosis became serious with the time of ligature. The expression of CTGF and α-SMA were consistent with the damage of tubulointerstitial. The positive correlation among CTGF and α- SMA or collagen protein were significant. Conclusion: These results indicated that the expression of CTGF was up-regulation obviously and had positive relationship with the expression of α-SMA and collagen protein. It was possible that CTGF participated in TIF and phenotypic transformation of tubular epithelial cells. Key words Fibrosis Connective tissue growth factor α-Smooth muscle actin Phenotype transformation Tubular epithelial cell Related Articles | Metrics

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Culture, identification, and labeling of rabbit mesenchymal stem cells in vitro 2010, 38 (9):  0-793 .  Abstract ( 1181 )   PDF (1340KB) ( 2023 )   Abstract Objective To establish a method of isolation, culture, identification and labeling rabbit mesenchymal stem cells (MSCs) in vitro. Methods MSCs were isolated and cultivated by density gradient and adherent methods. The expressions of CD29 and CD106 of cells were analyzed by using Immunocytochemistry. The third generation of MSCs were labeled by DiI，the labeling efficiency was detected. Results　Primary cultured MSCs adhered to plastic surface within 48h and reached 90% confluence within 7-8 d . MSCs expressed CD29 and CD106. All of the MSCs after labeling by DiI showed red fluorescence by immunofluoroscope. DiI labeling was sensitive and highly efficient to MSCs. Conclusion The method is simple and easy to isolate and cultivate MSCs and it can serve as a routine method. The culturing cells could be used in urethral construction of tissue engineering and then could be used to treat severe hypospadias、hypospadias cripples and urethral stricture in the future. Related Articles | Metrics

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Relationship between small low-density loipoprotein particles and plasm homocysteine level in coronary heart disease 2010, 38 (9):  0-793 .  Abstract ( 897 )   PDF (1167KB) ( 2269 )   [Abstract] Objection To investigate the relationship between small low-density loipoprotein particles and homocysteine in coronary heart disease(CHD). Methods Plasm lipids level ,small dense LDL percentage of total LDL and homocysteine level were measured in 109 patients who identified by coronary angiography, We explored the relationship between small, dense LDL level and homocysteine in different groups. Results The levels of TC,TG,LDL,small LDL and homocysteine were significantly higher in patients with CHD than those in controls （P<0.05）.The level of HDL-C were lower in patients than those in controls （P<0.05）.As the stenosis vessels increased, the sLDL and homocysteine leves also increased, Both sLDL and homocysteine leves might have positive correlation with the degree of coronary artery stenosis，we also found a positive correlation between sLDL and homocysteine. Conclusion there are positive correlation between sLDL and homocysteine .Both sLDL and homocysteine leves might have significan relationships with the degree of coronary artery stenosis. Related Articles | Metrics

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Investigation on the Biological Performance of Silk Protein 2010, 38 (9):  0-793 .  Abstract ( 1400 )   PDF (1810KB) ( 2042 )   Abstract Objective: To detection bacteriostatic activity, uvioresistant ability, hygroscopicity, moisture and dermal sensitization of silk protein and to furnish information for product development of silk protein. Methods: Silk protein was divided into 5 groups of varying size according to artificial, and prepared to solution. To detection related biologic activity by using plate bacterial colony count, absorption, moisture absorption rate of saturated calcium carbonate solution, allergen exposure and stimulate contact. Results: The silk protein had certain antibacterial ability, and the effect of growth inhibitory was excellent on E. coli than Staphylococcus aureus. The uvioresistant time of silk peptide, 3 μm silk protein powder and high molecular weight silk peptide lasted more than 1h. High molecular weight silk peptide and silk peptide had better hygroscopic, and hygroscopicity rate after 48 h were 44.78% and 35.30%. 3 μm silk protein powder and silk peptide had better moisture retention and moisture rate after 48 h were 82.29% and 78.91％. Silk protein was non-allergenicity on guinea-pig skin. Conclusion: The new cosmetics composed of silk protein may be researched and developed. Related Articles | Metrics

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Expression and Correlation of PSMA, AR in Bladder Transitional Cell Carcinoma 2010, 38 (9):  0-793 .  Abstract ( 1142 )   PDF (467KB) ( 1997 )   Abstract Objective: To investigate the expression and clinical significance of PSMA and AR in bladder transitional cell carcinoma (BTCC) and their relativity. Methods: Immunohistochemistry (S-P method) was used to detect the expression of PSMA, AR in BTCC (n = 75) and normal bladder mucosa (n = 15). Results: No expression of PSMA and AR was detected in normal bladder mucosa , and the positive expression rates of PSMA and AR in 75 cases of BTCC were 28.0 %, 21.3 %, respectively. Between in BTCC and in normal bladder mucosa, the difference of the positive expression rates of PSMA, AR were both statistical significance (P < 0.05). The positive expression of PSMA was not associated with the clinical stages and pathological grade, but was associated with whether the tumor was recurrent, with the statistical analysis revealed they had significant difference (P <0.01). The positive expression rate of AR in the BTCC had a tight relationship with the clinical stage of the tumor, and there was significant difference between the two stages(P <0.05). There was a positive correlation between the expression rates of PSMA and AR (r=0.328, P<0.01). Conclusion: The expression of PSMA and AR may be regarded as important markers in biological characters and prognosis of BTCC, and they may interact with one another in the development of BTCC. Related Articles | Metrics

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Analysis of 600 OSAHS patients with endotracheal intubation under guidance of bronchofibroscope through nose. 2010, 38 (9):  0-793 .  Abstract ( 1148 )   PDF (1007KB) ( 1847 )   [Abstract] Objective To study the clinical value of OSAHS patients with endotracheal intubation under guidance of bronchofibroscope through nose.methods The authors choose 600 OSAHS under the under guidance of bronchofibroscope through nose, and also choose 216 OSAHS through nose or mouth endotracheal intubation in induction of anaesthesia as the comparison. Results The sex （χ2 =1.43）, age （t=1.26）, obesity proportion （χ2 =1.65）, grave OSAHS proportion（χ2 =1.53） and the complication proportion（χ2 =1.37） have no signficant difference （P>0.05）. The treat group has difficult airway 384 case , 64.0%, and the comparison group has difficult airway 144 case, 66.67%, （χ2 =1.76），they have no signficant difference（P>0.05）. All the patients of the treat group are successful, but the comparison group can only reach the proportion of 81.94%, （χ2 =8.32）,have signficant difference （P<0.01）.Conclusion The technigue of endotracheal intubation under guidance of bronchofibroscope through nose has many advantages, it can deal with the difficult airway easily, has great clinical value. Related Articles | Metrics

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Significance of Determination of Changes of red cell immune function in IgAN 2010, 38 (9):  0-793 .  Abstract ( 1547 )   PDF (921KB) ( 1813 )   [Abstract] Objective To explore the significance of changes of red cell immune function in IgA nephropathy.. Method Forty-six type IgA nephropathy with biopsy-proven IgA nephropathy were used in this study. Flow-cytometry was applied in analysis changes of red cell of CD35 . Results: With the progress of IgA nephropathy, CD35 decreased gradually. CD35 expression did not correlate with proteinuria, but. distinctly associated with the level of serum creatinine, mesangial proliferative glomeruli and glomerulosclerosis(r=0.552,P<0.05，r=0.561,P<0.05，r=0.637,P<0.01，). Conclusion The abnormal expression of CD35 may contribute to the progression of IgA nephropathy. Related Articles | Metrics

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Treatment of traumatic brain injury in rats by transplanting of BMSCs with NgR gene Silencing by vein wang dong 2010, 38 (9):  0-793 .  Abstract ( 1336 )   PDF (1406KB) ( 1850 )   【Abstract】Objective To determine whether the NgR gene silencing in BMSCs can enhance curative effects of transplantation by vein of BMSCs on cerebral infarction in rats . Methods BMSCs were cultured by the suspension culture in vitro, and were transfected by siRNA to knock down the expression of NgR. Western blot and were used to assess the knockdown efficiency. The models of cerebral infarction were made by Middle cerebral artery occlusion (MCAQ) and randomly divided into three groups, A group for the NgR gene silencing of BMSCs .B Group for the equivalent group of BMSCs suspension. C group was injected the same amount of non-stem cell of the culture medium. The neurological defect scores were determined after 24 h, 3 days and 1, 2 weeks after MCAQ. The immunohistochemistry and pathology were checked after 2 weeks . 【Result】Western blot approved that the expression of NgR was knocked down by transfection of siRNA at 24 hours after the transfection. The neurological defect scores were significantly lower in A group than those in the B and C group 1 weeks after MCAQ (P<0.05). The number of neurons in A group was higher than that in the B and C group(P<0.05) .Conclusion: The BMSCs of NgR gene silencing transplanted by vein into the cerebral infarction tissues can significantly improve the neurological function in the rats with TBI Related Articles | Metrics

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The Prevalence of Lower Extremity Peripheral Arterial Disease in Patients with Cerebral Ischemic Stroke 1, 2010, 38 (9):  0-793 .  Abstract ( 1033 )   PDF (990KB) ( 1844 )   【Abstract】Objective:To access the prevalence of peripheral arterial disease (PAD) in hospitalized patients with cerebral ischemic stroke (CIS) by measuring the ankle-brachial index (ABI). Methods:Chinese patients of cerebral ischemic stroke were invited to participate prior to hospital discharge. Data regarding cerebral ischemic stroke risk factors, history of previous PAD, and ABI were collected. A total of 74 patients (41 men and 33 women) were recruited. Results: 20 patients were found to have PAD (mean ABI =0.831±0.079). There were significant differences in statistics between PAD and non-PAD group in diabetes mellitus(P=0.001) and FIB（P=0.001）.However, there were no significant differences in smoking history (P=0.228)、hypertension (P=0.359)、C-reactive protein (CRP)（P=0.339）、CHOL（P=0.414）、HDLC（P=0.461）、LDLC（P=0.054）、TG（P=0.235） and LP（a）（P=0.252）。Conclusion: Identification of patients with PAD by measuring the ankle-brachial index is easily done. Hospitalized patients with CIS are likely to have concomitant PAD. PAD in patients with CIS is more popular in these patients with advanced diabetic and high Fibrinogen. Related Articles | Metrics

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Pulse radiofrequency thermocoagulation for treatment of Idiopathic Trigeminal neuralgia by Stereotactic instrument 2010, 38 (9):  0-793 .  Abstract ( 1184 )   PDF (978KB) ( 1893 )   Objective To explore the etiopathogenisis of idiopathic trigeminal neuralgia(TN) and the joint technique of Stereotactic and Radiofrequency thermocoagulation. Methods The foramen ovale was localized under CT scan and penetrated guided by CT stereotactic. 26 patients was treated by radiofrequency thermocoagulation, and compared with others treatments. Results Successful penetrations when guided by CT stereotactic.and satisfied treatments by Radiofrequency thermocoagulation were 100 percent were obse-rved in all patients.The complication was 8.6%(3 monthes) and 10.3%(1 year). Conclusions Pulse radiofrequency thermocoagulation for treatment of Idiopathic Trigeminal neuralgia by Stereotactic instrument is a ecurity and efficacious treatment and able to eli-minate complications. Related Articles | Metrics

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Suppression Eeffect of RNA Interference on Expression of Skp2 of SKOV3 Ovarian Carcinoma Cell Line 2010, 38 (9):  0-793 .  Abstract ( 1012 )   PDF (1329KB) ( 1994 )   Objective To investigate suppression effect of RNA interference (RNAi) on expression of Skp2 of SKOV3 ovarian carcinoma cell line, The research is expected to reveal the relationship between Skp2 and cell cycle regulation and establish the theory basis for target gene therapy. Methods The Skp2-siRNA was designed and synthesized by Shanghai Gene Pharma Co., Ltd，and then transfected to SKOV3 ovarian carcinoma cell line. The cell line were divided into six groups, including the blank control group, the siRNA transfection group 1, the siRNA transfection group 2, the mock transfection group, the negative control group and the positive control group. The expression level of Skp2 mRNA and protein were detected by Realtime PCR and Western Blotting, respectively. The growth and proliferation of SKOV3 ovarian carcinoma cell line was observed with CCK-8 assay, results were analyzed statistically by ANOVA. Results After transfection with Skp2-siRNA, the expression level of Skp2 mRNA and protein in the siRNA transfection group 1 and group 2 were down-regulated, the growth and proliferation of SKOV3 Ovarian Carcinoma Cell line in the siRNA transfection group 1 and the siRNA transfection group 2 were inhibited, There were difference in the siRNA transfection group 1 and group 2 from in the blank control group (p<0.05), but no statistics difference between in the siRNA transfection group 1 and in the siRNA transfection group 2 (p>0.05). Conclusions 　The down-regulation of Skp2 expression of SKOV3 ovarian carcinoma cell line by Skp2-siRNA can lead to the suppression of proliferation, the small interfering RNAs technique can inhibit the proliferation of carcinoma cell by gene silencing of oncogene. Related Articles | Metrics

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The Change of Plasma Visfatin Level in Type 2 Mellitus Diabetes with Nonalcoholic Fatty Liver Disease 2010, 38 (9):  0-793 .  Abstract ( 1394 )   PDF (1435KB) ( 1837 )   Objective: To investigate the relationship between changes of the plasma visfatin level and the other indexes of metabolic and biochemery in type 2 diabetes mellitus with or without nonalcoholic fatty liver disease.Methods: Plasma visfatin level were assayed by ELISA in all subjects.The relationship of plasma visfatin level with blood glucose、blood lipids、HbA1c、FINs、WHR、BMI、HOMA-IR were also analyzed. Results: Plasma visfatin level in the two groups of type 2 were significantly increased compared with controls. Plasma visfatin level was positively correlated with HOMA-IR、Fins、P2hBG and was positively correlated with HOMA-IR、Fins、FBG、 P2hBG、LDL.Multiple regression analysis showed that HOMA-IR、P2hBG、LDLwere independent related fators in influencing plasma visfatin level.Multiple regression analysis showed that HOMA-IR、P2hBG、LDL were independent related fators in influencing plasma visfatin level .Conclusion: The change of plasma visfatin level is associated with the sensitivity of insulin,which may contribute in part to the pathogenesis of type 2 diabete mellitus and nonalcoholic fatty liver disease. Related Articles | Metrics

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Assessment of propofol intravenous anesthesia in clinical application of gastroscopic removal of foreign bodies in esophagus 2010, 38 (9):  0-793 .  Abstract ( 1611 )   PDF (1063KB) ( 2233 )   Assessment of propofol intravenous anesthesia in clinical application of gastroscopic removal of foreign bodies in esophagus Abstract: Objective To evaluate the safety and effectiveness of propofol intravenous anesthesia in clinical application of gastroscopic removal of foreign bodies in esophagus. Methods Twenty-four patients using propofol intravenous anesthesia during gastroscopic removal of foreign bodies in esophagus were analyzed retrospectively and thirty similar patients using normal methods were considered as controls. Results Foreign bodies in esophagus in all patients using propofol intravenous anesthesia were successfully removed without any serious effects. Six patients could not carry out in control group and foreign bodies in esophagus in five of them returning to use propofol intravenous anesthesia were successfully removed. SBP, HR and R of patients using propofol intravenous anesthesia were significantly decreased and SpO2 were higher than those of control patients during treatment（P<0.05）. No significantly difference was found in SBP, HR, R and SpO2 of two groups after treatment（P>0.05）. Conclusion Propofol intravenous anesthesia in application of gastroscopic removal of foreign bodies in esophagus is a good method with safeness and high success rate which is worth extending to further clinical use. Key words: Oesophageal foreign body ；Propofol；Painless gastroscopy Related Articles | Metrics

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Effects of Salvianolic Acid B on Atherosclerotic Plaque Area and Expressions of AGEs/RAGE in ApoE-Gene Knock-out Mice Treated with STZ and High Fat Diet 2010, 38 (9):  0-793 .  Abstract ( 1179 )   PDF (1663KB) ( 1929 )   Abstract Objective To observe the effects of Salvianolic acid B(SalB) on plaque area and AGEs/RAGE expression of plaque in diabetic atherosclerosis animal models prepared from apolipoprotein E(ApoE) gene knock-out mice treated by intraperitoneal injection of STZ and high fat diet. Methods Fifty two-month female ApoE gene knock-out mice were injected with STZ and fed with high fat diet for 12 months. Then blood sugar level was measured and forty diabetic atherosclerosis mice were selected for the following treatment experiment. Diabetic atherosclerosis mice were randomly divided into four groups. Each group was fed with same high fat diet and intragastrically administrated with different drugs for 8 weeks, named MOD group(distilled water)、SalBH(SalB 160mg/kg/d) group、SalBM group(SalB 80mg/kg/d) and Lovastatin（LVT 2.3mg/kg/d）group. Then fasting blood sugar level(FBG) was measured by using full automatic biochemistry instrument. Frozen sections and paraffin sections of mice aortas were made. The plague area、plaque area corrected by cross-sectional vessel wall area as well as the expressions of AGEs and RAGE were measured by Sudan Ⅲstaining or HE staining and immunohistochemistry staining and then analyzed by Image-Pro Plus(IPP) version 6.0 system. Results Compared with the MOD group, plaque area reduced significantly in SalBH、SalBM and LVT treatment group（P＜0.05）;besides plaque area of LVT group is lower than that of SalBH group and plaque area of SalBH group is lower than that of SalBM（P＜0.05）. Compared with the MOD group, average optical density value of AGEs reduced significantly in SalBH、SalBM and LVT treatment group（P＜0.05）. Conclusions SalB can reduce the diabetic atherosclerotic plaque area by decreasing the expressions of AGEs and RAGE in atheromatous plaque. Related Articles | Metrics

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The expression of EBP50、AKT2 in human breast cancer tissue and its significance shi zhimin 2010, 38 (9):  0-793 .  Abstract ( 1153 )   PDF (609KB) ( 1923 )   Abstract:Objective　To investigate the expression of EBP50、AKT2in human breast cancer tissue, study their relationship with clinicopathological factor in breast cancer, and analyze their expression significance and their correlation with each other.Methods　The expression of EBP50、AKT2 in 56 cases of breast carcinoma and 30 cases of adjacent nontumor normal breast tissue was detected by SP immunohistochemistry. Results　①The postitive expression of EBP50、AKT2 in 56 cases of breast carcinoma were 30.36% and 76.79% respectively.There was a significant different between carcinoma and normal breast tissue(P< 0.05);②The overexpression of EBP50 in breast carcinoma had no relationship with the size of primary tumor, age and pathological type, but had some relationship with histological classification, clinical TNM staging, and lymph node metastasis (P<0.05); AKT2 expression was inversely correlated with lymph node metastasis (P<0.05), had no relationship with age, size of primary tumor, histological classification, clinical TNM staging and pathological type (P>0.05).③EBP50、AKT2 showed significant indirect correlation with each other invasive ductal carcinoma. Conclusion The overexpression of EBP50、AKT2 may play a important role in the carcinogenesis and progression of breast carcinoma. They may be as the indications of biological behavior and prognosis of breast carcinoma. Related Articles | Metrics

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Clinicopathologic significance of the expression of thymosinβ10 protein in human esophageal carcinoma 2010, 38 (9):  0-793 .  Abstract ( 1204 )   PDF (758KB) ( 1833 )   ABSTRACT　AIM: The thymosin originally isolated from calf thymus, recently, it has been demonstrated overexpression in various neoplastic tissues. This study was to investigate the clinicopathologic significance of the expressions of thymosinβ10 in human esophageal carcinoma. METHODS: Immunohistochemical（IHC）staining was used to detect the expression of thymosinβ10 in 70 cases of esophageal carcinoma and 30 cases of para-cancerous normal esophageal mucosa, the results in combination with common clinicopathological parameters were statisticly analyzed. RESULTS: Thymosinβ10 expressed in the cytoplasm. The positive expression rate of thymosinβ10 in esophageal carcinoma 75.71%（53/70） was much higher than that in para-cancerous normal esophageal mucosa 23.33%(7/30) (P<0.01). The positive expression rate of thymosinβ10 in well differential esophageal carcinoma was much lower than that in moderate or poor carcinoma, the positive expression rate of thymosinβ10 in esophageal carcinom of mucosa or submucosa was much lower than that in muscularis propria carcinoma，the positive expression rate of thymosinβ10 in esophageal carcinom of negative lymph node metastasis was much lower than that in positive lymph node metastasis (allP<0.05). Thymosinβ10 expression had no relation with sex, age, site and size（P>0.05）. CONCLUSION: Thymosinβ10 was highly expressed in esophageal carcinoma, it was correlated with the differentiation, invasion and metastasis of esophageal carcinoma, it might be a new tumor marker for diagnosing of the human esophageal carcinoma. Related Articles | Metrics

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The Study on Effect of Mifepristone on PR、ER Expression and MVC Variation of Adenomyosis 2010, 38 (9):  0-793 .  Abstract ( 1217 )   PDF (1103KB) ( 1881 )   [Abstract] Objective To investigate variation and correlation of estrogen receptor(ER), progestin receptor (PR) and micro vessel count (MVC) in adenomyosis sites after treatment by small dose mifepristone. Method Experimental group (A) included 28 cases with adenomyosis classified manifestation and ultrasound diagnosis and identifyied by post operation histological section diagnosis receipted mifepristone 12.5mg/d for three months. The control group(B) had 30 patients without any ovarian hormone drug treatment. All specimen was treated by same method. ER, PR and MVC in the two groups were detected by immunohistochemical assay, and analyze the relation among them. Result There were 21（75%） positive cases and 26（86.67%） cases of ER protein in group A and group B respectively，there were difference between group A and group B in ER expression（P＜0.05)．There were 22（78.57%） positive cases and 29（96.67%） cases of PR protein in group A and group B respectively，there were obvious difference between group A and group B in PR expression.( P＜0.005).Micro vessel count of group A(75.1±7.8,total of 5 scopes 200 ×) were less than that of group B (90.4±8.7, total of 5 scopes 200 × ), (P＜0.05);the average area of micro vessel of group B(2334±129.4, total of 5 scopes 200 ×) was more than that of group A (783.3±54.2, total of 5 scopes 200 ×), there were obvious difference, (P＜0.001).Conclusion Small dose mifepristone could regular ER,PR expression negative in　adenomyosis．Mifepristone may play role in treating adenomyosis by inhibiting angiogenesis and reducing micro vessel area．. Related Articles | Metrics

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Therapeutic effect evaluation of different dose interleukin-2 in immunotherapy of locally advanced renal cell carcinoma after radical nephrectomy 2010, 38 (9):  0-793 .  Abstract ( 1745 )   PDF (983KB) ( 2664 )   Abstract Objective: To evaluate the efficacy and safety of a regimen of low-dose interleukin-2 (IL-2) in the treatment for patients with locally advanced renal cell carcinoma (LA-RCC). Methods: We retrospectively analyses the records of all 38 patients who underwent radical nephrectomy which have been certified LA-RCC in our hospital from March 2003 to March 2006. Patients be sub grouped to three groups and group A, B and C received 18 MIU/M2, 1MIU/M2 and 0.5 MIU/M2 IL-2 subcutaneously QOD. Every three month all patients were examination by ultrasound, chest roentgenograms. Groups were compared using Student's t-test, and a probability (P) value of less than 0.05 was taken to indicate statistical significance. Results: 5，11 and 16 patients of group A,B and C complete the treatment at last. There was no significiantly difference among the three groups in the rate of tumor recurrence, tumor metastasis and death (P＞0.05). the side effect of IL-2 in group B and C was significiantly less than group A (P＜0.05). Conclusion: Adminstered low-dose IL-2 may be a safe and effective option to LA-RCC, especially for low-risk LA-RCC patients. Key words: Interleukin-2, Renal cell carcinoma, Immunotherapy Related Articles | Metrics

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The Application of anterograde Tubal Typical ileum fistulation for obstructed left–sided colorectal cancer by resection with one-stage anastomosis 2010, 38 (9):  0-793 .  Abstract ( 1657 )   PDF (1025KB) ( 2066 )   Abstract Objective: To evaluate the clinic application of anterograde ileum tubal fistulation for obstructed left–sided colorectal cancer by a one-stage resection and an anastomosis. Methods: Studied Clinical data of 20 patients with obstructed left-sided colorectal cancer who underwent ileum tubal fistulation after A one-stage resection and an anastomosis. After the decompressive procedure, A catheter was inserted to terminal segmen of the ileum through the stump, on-table lavage, resection, and primary anastomosis be performed, then the catheter was fixed on abdominal wall.Results: There were no complication such as anastomotic leakage, postoperational stool leakage and peritonitis. The stool diversion was efficient. No deaths occurred. Conclusion: The Application of anterograde Tubal Typical ileum fistulation for obstructed left-sided colorectal cancer by a one- stage resection and an anastomosis was safety and efficient. Related Articles | Metrics

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Catheter Interventional Treatment of systemic-to-pulmonary arterial shunt obstruction 2010, 38 (9):  0-793 .  Abstract ( 1185 )   PDF (387KB) ( 1913 )   【Abstract】 Objective: Creation of a systemic-to-pulmonary shunt is still the firstline treatment in neonates with ductdependent pulmonary circulation, or in patients with hypoplastic left heart syndrome as a part of the first stage of the Norwood sequence. Acute complications after such surgery, such as stenosis, thrombosis, or kinking, are potentially lifethreatening. These complications require immediate revision or exchange of the shunt. In this report, we discuss interventional treatment as an alternative to surgery in 3 patients with acute stenosis of a shunt. Methods:The age of the patients ranged from 4 to 6 months, and their weights ranged between 4.2 and 6.2 kg. In 1 patients, the shunts were modified Blalock-Taussig type, while the 2 patients had shunts from the right ventricle to the pulmonary arteries.Results:We implanted 5 coronary arterial stent systems in 4 interventions. The diameter of the balloon used for implantation had a ratio to the shunt of 1. to 1. All shunts were successfully reopened by stenting. During follow-up, 3 patients underwent further procedures on an elective basis. Conclusion: In our limited experience, implantation of stents is an effective treatment for complications of shunts. Related Articles | Metrics

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Applicated value of serum AFP-L3 variants assay for differential diagnosis of liver disease DING Xian 2010, 38 (9):  0-793 .  Abstract ( 1091 )   PDF (1001KB) ( 1896 )   Abstract 0bjective: To investigate the value of serum AFP-L3 variants assay for differential diagnosis of liver disease.Methods : serum AFP-L3 variants were isolated by micro centrifugal Column,and the contents of AFP and AFP-L3 were detected by Elcteochemo luminescence immuno assay(ECLIA). Result：When AFP-L3/AFP＞10% was used as a positive criterion ,in the cases of AFP＞400ng/ml and AFP＜400ng/ml, the positive rate of AFP-L3 were 79.0% And 75.9%, respectively in hepatocellular carcinoma(HCC), which in liver cirrhosis(LC)、chronic hepatitis(CH) and autoimmune hepatitis(AIH) were 8.7%、7.9% and 7.3% ,respectively.Conclusion ：AFP-L3 is very useful in the differential diagnosis of HCC and low AFP level liver disease. Related Articles | Metrics

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The Initial Study of Relationship between ATGL and Type 2 diabetes mellitus With Non-alcoholic Fatty Liver 2010, 38 (9):  0-793 .  Abstract ( 1604 )   PDF (1103KB) ( 1949 )   Objective: To explore the relationship between plasma ATGL and related metabolic indexes of Type 2 diabetes mellitus with fatty liver. Methods: Fasting plasma ATGL level of one hundred and thirty-one patients with incipient T2DM,T2DM group (seventy four patients without fatty liver, A group; fifty seven patients with fatty liver, B group) and forty four healthy subjects (NC group)were assayed by enzyme-linked immunosorbent asssy(ELISA),A comparison about basal materials and ATGL was made between A group 、B group and NC group, correlation of ATGL and basal materials were measured as well, then multiple stepwise regression analysis were made. Results: Fasting plasma ATGL level in T2DM without fatty liver was significantly lower than controls（P<0.01）,39.13?23.98ng/ml vs 69.21?27.05 ng/ml. Fasting plasma ATGL level in T2DM with fatty liver was significantly lower than controls（P<0.01）,18.25?14.85ng/ml vs 69.21?27.05 ng/ml. Fasting plasma ATGL level in T2DM with fatty liver was significantly lower than without fatty liver（P<0.01）,18.25?14.85 ng/ml vs 39.13?23.98 ng/ml. Correlation analysis showed that the fasting ATGL level was negatively in correlation with TC、TG、、Fins 、BMI、HOMA-IR（r=-0. 222 、-0.424、-0.317、-0.200、-0.178，P<0.05）. Multiple stepwise regression analysis showed that Fins、BMI、TC were independent related factors that influenced the level of plasma ATGL. Conclusion: ATGL may contribute to the pathogenesis of T2DM with non-alcoholic fatty liver. Related Articles | Metrics