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15 June 2012, Volume 40 Issue 6 Previous Issue    Next Issue
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论著
实验研究
短篇论著
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论著

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Construction of a p100 Stable Knock-downed HepG2 Cell Line with a p100 shRNA Expression Lentiviral System 2012, 40 (6):  529-532 .  Abstract ( 1432 )   PDF (51364KB) ( 2133 )   Objective: To construct a lentiviral vector expressing a short hairpin RNA (shRNA) which targets the functional p100 gene and to construct a p100 stable knock-downed HepG2 cell line with this p100 shRNA expressing lentiviral system. Methods: A double-stranded shRNA targeting the p100 gene was designed, synthesized and cloned into the pLKO.3G vector. Positive clones were verified with double enzyme digestion and sequencing. Then the valid constructions were cotransfected with the other 2 packaging plasmids into 293T cells to package lentiviral particles carrying p100 shRNA expression element. The lentivirus was then harvested and used to infect HepG2 cells. Infection efficiency was indicated with GFP and detected with fluorescent microscope. The knock-down efficiency of p100 in infected HepG2 cells was verified with western blot. Results: A lentiviral vector carrying a shRNA targeting the p100 gene was successfully constructed and a p100 stable knock-downed HepG2 cell line was established by using this lentiviral system. After viral particles infection of HepG2 cells, strong green fluorescence was observed in HepG2 cells under fluorescent microscope, the infection efficiency exceeded 90%. Western blot analyses confirmed that the expression of p100 was significantly down-regulated in this infected HepG2 cell line. Conclusion: A lentiviral vector carrying an shRNA targeting the p100 gene was successfully constructed and a HepG2 cell line stably expressing p100 shRNA was established with this lentiviral system. Related Articles | Metrics

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Construction the vector of pacA and gtfB-cat of Streptococcus Mutans fused with ctxB，and expression in prokaryotic cells 2012, 40 (6):  533-536 .  Abstract ( 1224 )   PDF (774KB) ( 2315 )   To constructe the chimeric vector with the encoding gene of PAcA of S.mutans, catalytic region in glucosyltransferase and cholera toxin B subnnit, and to testify its expression in Escherichia coli. Methods: Obtaining target genes pacA, gtfB-cat and ctxB with specific primers and templates of plasmids pcDNA3-pac, pET-gtfB and pBS-ctxB;Construction of intermediate vectors T-pacA, T-cat and T-ctxB through T-A cloning;T-ctxB and pET32-a(+) were digested with double restriction enzymes EcoRI and XhoI to construct the recombinant vector pET-ctxB, T-pacA and pET-ctxB were digested with KpnI and BamHI to construct the recombinant vector pET-pacA- ctxB. Finally, T-cat and pET-pacA-ctxB were digested with BamHI and EcoRI to construct the recombinant vector pET-pacA-cat-ctxB. Results: pacA, gtfB-cat and ctxB are all inserted into the vector pET32-a(+) correctly through the tests of restriction enzyme digestion, PCR and sequencing respectively. Their ORFs didn’t be changed. The recombinant plasmid can express target protein in E.coli host strain BL21 (DE3) after induced by IPTG, and the molecular weight of the protein were consistent with the prediction. Conclusion: The recombinant plasmid pET-pacA-cat-ctxB was successfully constructed and it can be expressed in prokaryotic cells. Related Articles | Metrics

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The Effects of dbcAMP on the Proliferation of SW579 Cell Line 2012, 40 (6):  537-539 .  Abstract ( 1249 )   PDF (470KB) ( 2146 )   Abstract Objective :To study the effect of dbcAMP on the Proliferation of SW579 Cell Line.Methods: SW579 cells were treated with dbcAMP (0.5,1.0,2.0 mmol/L) at 24 h. The growth activity of cells was detected by MTT; The activity of MPF through the ELISA; the protein express of the phosphorylation level of cdc2-Tyr15 by Western Blot; the change of cell cycle will be detected by FCM . Results :MTT showed that the growth activity of SW579 cells was reduced ; ELISA showed that dbcAMP could decrease the activity of MPF. The activity of MPF was (115.5±7.53) ng/L in group without dbcAMP ; While the activity of MPF was (22.87±2.57) ng/L in group with dbcAMP . Western blot showed that the content of cdc2-Tyr15 increased. After the treatment with 1.0 mmol/L dbcAMP for 24 h, the percentage of SW579 cell in G0/G1 phase was (62.50±1.22)%, in S phase was (10.15±0.38)%, in G2/M phase was (26.39±0.76)%; otherwhile in the control group, the percentage of SW579 cell in G0/G1 phase was (60.83±1.61)%, in S phase was (29.19±0.97)%, in G2/M phase was (8.75±0.50)%. Conclusion :dbcAMP could significantly inhabit the SW579 cells proliferation and arrest cell cycle in G2/M phase. The mechanism on inhibiting the growth of thyroid cancer maybe related to the decreasing activity of MPF. Related Articles | Metrics

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Expression of IL-6, IL-17, TGF-β in Cystitis Glandularis and their significances 2012, 40 (6):  540-542 .  Abstract ( 1403 )   PDF (20097KB) ( 2102 )   Abstract Objective: To study the expression of IL-6, IL-17 and TGF-β in the tissue of cystitis glandularis(CG), investigate the role of nonresolving inflammation in the pathogenesis and development of CG. Methods: Collect 15 specimens of normal mucous membrane of bladder as control group (Group A)，43 specimens of curative cystitis glandularis as (Group B); 13 specimens of recurrent and cancerate cystitis glandularis as progression (Group C); Immunohistochemical (SABC) method was used to detect the expression of interleukin-6(IL-6), interleukin-17(IL-17) and TGF-β in the 3 groups. χ2 test was used to analyze the positive rates of the 3 factors in the 3 groups; Spearman rank correlation test was used to analyze the dependability of the 3 factors. Results: Positive rates of IL-6, IL-17, TGF-β in Group A、B、C: Group A were 20%（3/15）、13.3%（2/15）、33.3%（5/15）; Group B were 60.5%（26/43），62.8%（27/43），51.2%（22/43）; Group C were 92.3%（12/13），100%（13/13），100%（13/13）.There were significant differences between the 3 groups (χ2=15.824、23.036、14.274；P=0.003、0.000、0.006); there were no significant differences in expression levels of 3 groups between the different age and gender(P >0.05). Positive correlation exited between IL-6, IL-17,TGF-β and progression（r =0.728、0.785、0.659；P=0.000、0.000、0.000）；positive correlation existed between IL-17 and IL-6,TGF-β（r=0.997、0.761；P=0.000、0.000）. Conclusion: Positive expression of IL-6, IL-17, TGF-β and the synergetic effect of the 3 factors may play important roles in the pathogenesis and development of CG, further more nonresolving inflammation may play an important role in the pathogenesis and development of CG. Related Articles | Metrics

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Cloning of the extracellular domain gene of human thyroid peroxidase and construction of it’s baculovirus expression vector 2012, 40 (6):  543-546 .  Abstract ( 1305 )   PDF (5831KB) ( 2174 )   Abstract Objective: Try to clone the extracellular domain gene of human thyroid peroxidase(hTPO) and construct the baculovirus expression vector of it, so that we can carry out it’s expression in the baculovirus-insect cell expression system in the future. Method: Human TPO’s extracellular domain gene was obtained by PCR method. The fragment was ligated with pGEM3zf(+) vector and pFastBac1 vector sequently to generate recombinant hTPO-pGEM3zf(+) and hTPO-pFastBAC1. The hTPO-pFastBAC1 was used to transfect E.coli DH10Bac so as to get the recombinant baculovirus expression vector(hTPO-Bacmid). For each step, we identify the accuracy by several methods. Results: By methods of PCR, restriction endonuclease analysis and DNA sequencing, the recombinant hTPO-pGEM3zf(+),hTPO-pFastBAC1 and hTPO-Bacmid was confirmed the same as predicted. Conclusion: We have cloned hTPO’s extracellular domain gene successfully, and then generated recombinant hTPO-pGEM3zf(+) and hTPO-pFastBAC1 accurately. The latter one achieved recombination with Bacmid by site-specific transposition. So the recombinant baculovirus expression vector of hTPO was obtained. This work has established a basis for hTPO gene’s eukaryotic expression in insect cells. Related Articles | Metrics

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Affection of hyperhomocysteinemia on sympathetic nervous fanction of 2 type diabetic mmellitus 2012, 40 (6):  547-549 .  Abstract ( 1302 )   PDF (372KB) ( 2496 )   Affection of hyperhomocysteinemia on sympathetic nervous function of 2 type diabetic mellitus WANG suhong, ZHANG zhecheng, LIU na, ZHU ju, ZHANG jing Department of Neurology, The Third Central Clinical College of Tianjin Medical University, Tianjin 300170,China Abstrict Object:To investigate affection of hyperhomocysteinemia（Hhcy）on sympathetic nervous function of 2 type diabetic mellitus（DM）by detection of sympathetic skin reflex（SSR）.Methods:44 patients with 2 type DM were divided two groups: Hhcy group(n=22) and NHhcy group(n=22).Detection of SSR were given to four limbs of all patients, record and compare average latencies、amplitudes and ratios of upper to lower limbs. Results: SSR upper limbs latencies in Hhcy group [(1593.11±149.85)ms] were prolonged than that in NHhcy [(1539.75±149.22)ms](p>0.05).SSR lower limbs latencies in Hhcy group [(2795.86±415.24)ms] were prolonged than that in NHhcy group [(2617.93±327.61)ms](p＜0.05). SSR upper limbs amplitudes in Hhcy group [(1.03±0.38)mv] were reduced than that in NHhcy group [(1.22±0.30mv) (p＜0.01).SSR lower limbs amplitudes in Hhcy group [(0.28±0.17)mv]were reduced than that in NHhcy group [（0.38±0.15)mv](p＜0.01). Amplitude ratios of upper to lower limbs in Hhcy group were higher than that in NHhcy group, z=3.5176, p＜0.001;but latency ratios of upper to lower limbs between 2 groups had not statistical significance。Conclusion：Hhcy may increase the damage to sympathetic nervous of 2 type DM; it has features of Length dependence, and detection of SSR provides Simple and feasible technique. Related Articles | Metrics

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research about the lesion characteristics of cervical erosion performance and HPV16/18 E6 expression 2012, 40 (6):  553-555 .  Abstract ( 1262 )   PDF (992KB) ( 2294 )   Abstract Objective：To investigate the pathology diagnosis of the cases who were diagnosed as cervical erosion by naked eyes and the expression of human papillomavirus type 16/18 E6 protein and it’s clinical significance. Methods:Choose the age of 18-65 cervical minor erosion group (30 cases)、moderate erosion group(41 cases)、serious erosion group(29 cases),smooth group 100 cases(the contrast group).Clearring those cases’ pathology organization diagnose. The expression of human papillomavirus type 16/18 E6 protein was detected by immunohistochemistry. Results: （1）The pathology organization diagnose result of every group are：normal、cervical inflammation、CINI、CINII、CINIII and cervical cancer.The constituent ratios of cervical intraepithelial neoplasia and cervical cancer in the three groups were becoming higher and higher, the differences of consitituent ratios were statistically significant（P<0.05）. The erosion group compare with the smooth group（P<0.001）.（2）The expression of HPV16/18 E6 among the erosion groups is higher than smooth group, the differences were statistically significant（P<0.05）。Compare serious and moderate，serious and minor ,both were statistically significant（P<0.05）．Among moderate and minor, the differences were not statistically significant（P>0.05）．Conclusion: In the naked eyes imagined cervical erosion cases,their organization diagnose are diversity,more higher it’s erosion,the possibility of developing into CIN or cervical cancer is more greater,the expression level of HPV16/18 E6 is more higher. Related Articles | Metrics

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The relationship between nocturnal hypoxemia and ventricular arrhythmia in patients with chronic obstructive pulmonary disease 2012, 40 (6):  556-559 .  Abstract ( 1350 )   PDF (419KB) ( 2216 )   Objective: The purpose of this study was to investigate the relationship between nocturnal hypoxemia and risk of ventricular arrhythmia (VA) during sleep in patients with chronic obstructive pulmonary disease (COPD). Method: 36 patients with COPD and 36 healthy subjects were selected . All the patients accepted Polysomnography, 24-h Holter electrocardiographic recording, Arterial blood gas analysis and pulmonary function tests. Results : Nocturnal hypoxemia episodes were found in nineteen patients with COPD during sleep but none of the control subjects . The prevalence of VA in patients with COPD was also significantly higher than that observed in healthy subjects( 58.3% vs 5.6% ,P<0.01). No associations were found between VA and nocturnal hypoxemia in patients with COPD (rho=-0.077,P=0.646). No differences were found between during REM and non-REM sleep for the frequency of VA[（5.51±0.53）times/h vs（5.58±0.32）times/h] . Conclusion：Patients with COPD have a higher frequency of VA and nocturnal hypoxemia episodes than health subjects. However, no associations were found between VA and nocturnal hypoxemia in patients with COPD. Related Articles | Metrics

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The Effect of Fluvastatin-Probucol Combined Therapy on Levels of Serum Lipids and high sensitive C-reactive protein in Patients with Acute Coronary Syndrome 2,2,CAO Yue-Juan Yong-Bin LI 2012, 40 (6):  560-562 .  Abstract ( 1358 )   PDF (337KB) ( 2209 )   Abstract Objective: To evaluate the effect of fluvastatin-probucol combined therapy on levels of serum lipid and hs-CRP in patients with acute coronary syndrome. Methods: A total of 60 patients with acute coronary syndrome were randomly divided into three groups, 20 patients in each group. The patients of group A were taken fluvastatin 40mg/d; the patients of group B were taken fluvastatin 80mg/d; and the patients of group C were taken fluvastatin 40mg/d and 250 mg of probucol twice a day. The serum levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) and hs-CRP were examined before treatment. All of them were detected again after 3 months of treatment. Results: There were no significant differences in the levels of serum lipid and hs-CRP before treatment among three groups (P>0.05). After the 3 months follow-up, the levels of serum lipid and hs-CRP in group C were lower than group A (P<0.05); the levels of TC and HDL-C were lower than group B (P<0.05). There were no differences in the levels of TG and LDL-C between C and B groups (P>0.05). Compared with the pretreatment values, the levels of TC and LDL-C in group B and C were significantly decreased after the therapy of 3 months (P<0.01) and the level of TG in group C was also reduced (P<0.05); the level of hs-CRP was decreased after treatment in each group (P<0.05), especially in the group C (P<0.01). Conclusion: Combined treatment by fluvastatin and probucol or single high-dose fluvastatin therapy has intensive effect on lowing serum lipid and hs-CRP in ACS patiants. Especially, the combined theatment could further strengthen the efficacy. Related Articles | Metrics

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Correlation of CEA、NSE、CYFRA21-1 and Pathological Staging with Postoperative Non-Small Cell Lung Cancer 2012, 40 (6):  566-568 .  Abstract ( 1576 )   PDF (309KB) ( 2962 )   Abstract Objective： To investigate the union expression of CEA、NSE and CYFRA21-1 in non-small cell lung cancer and to explore the relationship between the tumor markers and pathological staging of non-small cell lung cancer. Methods: 143 patients in non-small cell lung cancer with their postoperative pathological dates were collected .The expression results of CEA、NSE and CYFRA21-1 were measured before operation in all patients and then were used to study the correlation between them and postoperative pathological staging . Results: The levels of serum CEA and CYFRA21-1 in patients are different when T、N stages and group numbers of metastasis lymphnodes are not same (P＜0.05orP＜0.01).Levels in patients of N2 with multi- metastasis groups lymphnodes were significantly higher than those in patients of N1 and N2 with single-metastasis group lymphnode . The levels of serum NSE in patients of different T stages(T1 and T3) was significantly different (P＜0.05),but there 's same level in patients with the different pathological N stages(P＞0.05). Conclusion: Preoperative detection of the serum concentration of CEA and CYFRA21-1 has important clinical significance on the accurate judgment of tumor staging in patients with non-small cell lung cancer. Related Articles | Metrics

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Risk factors of vascular dementia after Cerebral Infarction 2012, 40 (6):  569-571 .  Abstract ( 1371 )   PDF (331KB) ( 2633 )   Abstract Objective: To examine correlation factors for vascular dementia （VD）after cerebral infarction. Methods: A total of 392 patients were enrolled, and related clinical data and laboratory examinations were collected. Risk factors for VD were determined by using multiple logistic regression analysis. Results: Eighty-four participants (21.4%) were diagnosed as VD. Univariate analysis found that VD was associated with number of lesions, location of lesions, leukoaraiosis and cerebral atrophy. Multivariate logistic regression suggested that left hemisphere or bilateral lesions (OR=8.958) 、number of lesions(OR=1.375) and cerebral atrophy(OR=2.297) were independent risk factors of VD. VD was unrelated to age, education, or vascular factors (hypertension, diabetes, coronary heart disease, or hyperlipidemia ). Conclusions: Vascular dementia is frequent after ischemic stroke. Cerebral small vessel disease (SVD) is a major cause of VD. Better knowledge of the risk factors for VD should be emphasized to reduce the attacks of vascular dementia. Related Articles | Metrics

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Application Study of Ipratropium in Fiberoptic Bronchoscopy 2012, 40 (6):  572-574 .  Abstract ( 1310 )   PDF (346KB) ( 2157 )   Abstract Objective：To study the application value of the inhalation of ipratropium in fiberoptic bronchoscopy. Methods: 88 patients undergoing bronchoscopy were enrolled in this study, 48 patients were divided into experimental group and inholed ipratropium 1 mg before bronchoscopy, including 24 patients in the normal lung function sub-group and 24 in the non-normal sub-group; control group of 40 patients, including 20 patients in the normal lung function sub-group and 20 in the non-normal sub-group. The patients of the four sub-groups undergoing bronchoscopy after lidocaine anesthesia. Compare the changes of the airway resistance index before and after the bronchoscopy, including the total airway resistance (R5), central airway resistance (R20), surrounding elastic resistance (X5) and the resonance frequency (Fres). Results: After bronchoscopy, R5, R20, X5 and Fres of the experimental group were reduced (P < 0.01). The changes of R5, R20, X5 between the two subgroups were not significant. In control group, the variations of airway resistance in the normal sub-group were not significant, R5, R20, X5, Fres were increased in the non-normal subgroups (P <0.01), and the change quantities were greater than normal sub-group. In the normal lung function group, R5, R20, Fres of the experimental group were more improvement, X5 was also improved, but the difference was not significant. In the non-normal lung function group, R5, R20, X5 and Fres of the experimental group were significantly reduced (P <0.05). Conclusion: Premedication with an inhaled ipratropium can reduce the airway resistance of patients undergoing bronchoscopy, which can reduce the incidence of bronchospasm. Related Articles | Metrics

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Study on Different Concentrations of Ozone Solution Acting on the HVB Virus in Dental Unit Waterlines 2012, 40 (6):  575-578 .  Abstract ( 1179 )   PDF (490KB) ( 2304 )   Abstract Objective: To study different concentrations of ozone solution which acc on the Hepatitis b virus (HBV) in dental unit waterlines (DUWLs) and find out the minimum ozone water concentration which can inactivate HBV in DUWLs. Methods: Culture the human 2215 cell. Under the room temperature 5℃、concussion mixed、airtight and so on conditions, prepare different concentrations of aqueous solution of ozone.Put the ozone solution into the 2215 culture flask. Different concentrations of aqueous solution of ozone act with the supernatant liquid of the human 2215 cell in different times.Extract the HBV-DNA from the 2215 cell supernatant. Fluorescence quantitative PCR amplification. Detect the contents of HVB-DNA. Results: under those conditions, the aqueous solution of ozone is stable. The concentration of ozone at 0.6mg/L in 10 minutes and the concentration of ozone at 0.5mg/L in 15minet can inactivate Hepatitis b virus at limited level. Conclusion: The concentration of ozone at 0.5~ 0.6mg/L can inactivate Hepatitis b virus in DUWLs. Related Articles | Metrics

实验研究

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The Study of E-cadherin Gene PromoterDemethylation in Nude Mice Burdended -ovarian Cancer 2012, 40 (6):  582-585 .  Abstract ( 1400 )   PDF (6359KB) ( 2171 )   Objective: To evaluate the effect of the DNA methyltransferase (DNMT) inhibitor 5 - aza -2 '- deoxycytidine (5-Aza-CdR) combined histone acetylation Enzymes (HDAC) inhibitor Sodium 4-Phenylbutyrate (SPB) on the expression of epithelial cadherin (E-cad) expression of nude mice models with ovarian cancer cell lineSKOV3. Methods: 40 nude mice were implanted ovarian cancer cell lineSKOV3 into abdominal cavity as animal model. The models were randomly divided into four groups. Controlled group was injected phosphate-buffered saline into abdominal cavity, 5-Aza-CdR and SPB were used respectively in other two groups, and 5-Aza-CdR plus SPB were used in the fourth group. After two weeks of injection, the expressions of DNMT1, HDAC1 and E-cad in the ovarian cancer xenograft tumors by immunohistochemical staining. The CpG island methylation status of E-cadherin promoter region in xenograft tumors were analyzed by methylation specific PCR (MSP). The mRNA and the protein expressions of E-cad were also compared among the four groups. Results: DNMT1 and HDAC1 were highly expressed in xenograft tumors. 5-Aza-CdR can inhibit the expression of DNMT1 and SPB can inhibit the expression of HDAC1 which were indicated by comparison with controlled group (P <0.05). The combining 5-Aza-CdR with SPB have no obvious synergistic effect on the inhibition of DNMT1 and HDAC1. 5-Aza-CdR can induce methylated E-cad gene demethylation which made E-cad mRNA and protein expressions recovered or even better. 5-Aza-CdR combined with SPB can induce methylated E-cad gene demethylation and recover the E-cad mRNA and protein expressions compared with other groups. Conclusion: 5-Aza-CdR can reduce the E-cad gene promoter methylation and make E-cad mRNA and protein expressions recovered. 5-Aza-CdR combined with SPB can accomplish synergetic effect and enhance the expression of methylated E-cad gene significantly which played an important role in the treatment of ovarian cancer Related Articles | Metrics

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The modeling of isolating rabbit left atrial myocytes and analysis of electrophysiological characteristics 2012, 40 (6):  586-589 .  Abstract ( 1219 )   PDF (2681KB) ( 2228 )   Objective: To study the method of acute isolating rabbit left atrial myocytes and analysis the electrophysiological characters. Methods: Left atrial myocytes of 10 rabbits were isolated by perfusion of coronary artery and enzymatic dissociation. Whole-cell patch-clamp technique was used to record action potential (AP) and ionic channel currents (ICaL and INa). Results: Single rabbit left atrial myocyte with normal morphology was obtained．AP and the membrane currents of ICaL and INa were successfully recorded．The resting potential averaged respectively -62.8±2.4mV. APD90 and APD50 decreased as stimulation frequency increased. APD90 and APD50 of 4Hz or 5Hz were significant differences compared with 1Hz of stimulation frequency (P<0.05). The densities of ICaL were -4.79±1.28pA/pF, activited potential was -40mV, potential of peak current was +10mV, reversed potential was +40～+50mV. The densities of INa were -118.41±16.67pA/pF, activited potential was -60mV, potential of peak current was -40mV, reversed potential was +20～+30mV. Conclusion: These results suggest that, left atrial myocytes of dissociating model own normal electrophysiological activity and features, which can be used to study atrial electrical and ionic channel remodeling of atrial fibrillation． Related Articles | Metrics

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The Effect of the Celecoxib on memory function and the expression of Caspase-3 protein after traumatic brain injury . 2012, 40 (6):  590-593 .  Abstract ( 1285 )   PDF (16410KB) ( 2080 )   Objective This is a study on the level of molecular biology to clarify the change regulation of inflammatory reaction and to investigate the effect of cyclooxygenase -2 inhibitor Celecoxib on memory function and the expression of Caspase-3 protein after traumatic brain injury (TBI) .Methods The model of severe closed TBI was established according to the method created by Marmarou. Adult Wistar rats (n=48) were randomly divided into TBI group , Celecoxib treatment group , sham operation group, normal controls. All rats were killed at each time point ,for detective COX-2 and Caspase-3 protein expression with West-blot and immunohistochemistry. Another 24 rats were randomly divided into TBI group , Celecoxib treatment group , sham operation group , and normal group . The cognitive dysfunction was evaluated using the Morris water maze (MWM) . Results The increased expression of protein COX-2 and Caspase-3 in brain after TBI, Celecoxib can apparently decrease COX-2 and Caspase-3 protein expression in brain, Celecoxib treatment group is compare with TBI group in distinction bears significance in statistics（p＜0.05）, and it can obviously shorten the latency of Morris water maze tests in 9、10day,the distinction bears have a significance in statistics（p＜0.01）.Conclusion Celecoxib can apparently decrease the expression of COX-2 and caspase-3, and inhibition of inflammatory reaction and apoptosis ，obviously shorten the latency of Morris water maze tests. Related Articles | Metrics

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Effect of Schisandra Chinensis Mixture on Expression of MCP-1 and iNOS in Renal Tissues of Mice withDiabetic Nephropathy 2012, 40 (6):  594-597 .  Abstract ( 942 )   PDF (3243KB) ( 2641 )   Abstract Objective:To investigate the effect of schisandra chinensis on the expression of MCP-1 and iNOS in renal tissue of mice with Diabetic Nephropathy induced by streptozotocin(STZ). Methods:Twenty-four male C57BL/6 mice were randomly divided into three groups:normal control group,diabetic model group and SM-treated group.The mouse model of diabetes was induced by STZ intraperitoneally.The serum level of glucose,body weight and urinary albumin creatinine ratio(UACR) were measured. Furthermore,the morphological changes of renal tissue were observed under microscope.The expression of mRNA in kidney tissues were determined by RT-PCRl method.The protein expression in kidney tissues were determined by immunohistochemical method. Results:Compared with the diabetic model group,there were significantly lower level of serum glucose and UACR in SM-treated group,SM could also reduce the extracellular matrin accumulation in glomerular mesangial region and renal tubule and interstitial area. There were lower protein and mRNA expression of MCP-1 and iNOS in SM group.,too(P<0.05).Conclusion:After treatment of SM, the expression of MCP-1 and iNOS were decreased, which may reduce renal inflammation and relieve renal injury. Related Articles | Metrics

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Experimental Study of Endothelial Progenitor Cell’s Differentiation to Endothelial Cell on Corpus Luteum of Pseudocyesis Rat 2012, 40 (6):  597-581 .  Abstract ( 1350 )   PDF (15002KB) ( 2046 )   Abstract:Objective To observe bone marrow endothelial progenitor cell’s (EPC) differentiation to endothelial cell on corpus luteum of pseudocyesis rat and the participation in vasculogenesis;Methods From caudal vein, female rats that rediated by 137Cs were transplantated EPCs marked DAPI of male rats, made pseudocyesis model of transplantated female rats after hematopoietic reconstitution,then on the 7th day, observed EPCs’ distribution and differentiation on the female rats’ bone marrow, peripheral blood and corpus luteum by fluorescence microscopy and in situ hybridization SRY;Results cells marked DAPI dispersed on models’ bone marrow, peripheral blood and corpus luteum, and male rat’s EPCs marked SRY appeared on the capillary of model rats’ corpus luteum; Conclusions EPCs start differentiation to endothelial cells and participate in vasculogenesis on pseudocyesis rats corpus luteum. Related Articles | Metrics

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Studies on Anti-Inflammatory Mechanisms of Plantain Seed Polysaccharides 2012, 40 (6):  598-601 .  Abstract ( 1312 )   PDF (454KB) ( 2684 )   Objective To investigate the anti-inflammatory effect of the plantain seed polysaccharides (PSP). Methods Various inflammatory models, including the swelling of ear induced by xylene in mice, the permeability of blood capillary by acetic acid in mice, viewing earlier period Anti-inflammatory Effect of PSP. Then, air sac models of synovitis in mice were set up, the amount of white blood cell (WBC), the volume and the contene of tumor necrosis factor (TNF-a) were measured in inflammatory exudan, the activity of superoxide dismutase (SOD), the contene of malondialdehyde (MDA) were measured in inflammatory exudan and blood serum to observe intermediate stage anti-inflammatory effect of PSP and the influence on above-mentioned index. Meanwhile, through the cotton-pellet granuloma of the mice, viewing advanced stage anti-inflammatory effect of PSP. Results The PSP had significant inhibitory action on auris swell induced by xylene in mice, penetration of capillary vessel in mice and the proliferation of granuloma caused by implantation cotton-pellet in mice. The amount of WBC 、the volume 、the contene of tumor necrosis factor(TNF-a) in inflammatory exudan, and the content of MDA in inflammatory exudan and blood serum were obviously lower than those in the control groups. The activity of SOD was obviously higher. Conclusion The PSP has significant anti-inflammation, which is relevant to the inhibition of the effusion on the inflammatory factor, the elimination of the free radicle and inhibition of the lipid peroxidation. Related Articles | Metrics

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Effect of Telmisartan on the Adipoeytokines as Omentin、Retinol binding protein 4 and Visfatin in OLETF Rats with lnsulin Resistance 2012, 40 (6):  605-608 .  Abstract ( 1231 )   PDF (456KB) ( 2230 )   Abstract Objective：To investigate the mechanism of angiotensin II type＼(AT1)receptor blocker，telmisartan in OLETF rats with insulin resistance．Methods：Forty-seven male OLETF rats were fed with high-fat diet for 22 weeks to establish the insulin resistance model with hyperlipidemia．Forty-seven OLETF rats with insulin resistance models were randomly assigned into five groups，the model group，in which the rats were fed with high-fat diet；the telmisartan group，in which the rats were fed with high-fat diet and telmisartan；the metformin group in which the rats were fed with high-fat diet and metformin；the pioglitazone group，in which the rats were fed with high-fat diet and pioglitazone ；the low pioglitazone group，in which the rats were fed with high-fat diet and low pioglitazone .After 26 weeks of drug intervention，the plasma levels of fasting blood glucose(FBG)，fasting insulin(FINS)，free fatty acids(FFA)，lipids，omentin，retinol binding protein 4 and visfatin were detected in five groups．Results：After 26 weeks of telmisartan treatment，the insulin resistance and lipid metabolism were improved in OLETF rats．The serum omentin was significantly higher in the telmisartan group than that of the model group(F=6.888，P<0.001). The serum retinol binding protein 4 and visfatin were significantly lower in the telmisartan group than that of the model group(F=7.236，P<0.001 and F=4.165，P=0.003)．The serum lipid level and insulin resistance were significantly improved．Conclusion：Telmisartan can improve blood glucose and insulin resistance by inhibiting the expression of retinol binding protein 4 and visfatin ；enhancing the expression of omentin and regulating serum lipid leve1. Related Articles | Metrics

短篇论著

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The effect of different revascularization treatment on clinical outcomes in patients with coronary artery disease and type 2 diabetes 2012, 40 (6):  609-611 .  Abstract ( 1175 )   PDF (348KB) ( 2289 )   Abstract Objective：The study was performed to discuss the selection of different revascularization treatment in patients with coronary artery disease and type 2 diabetes. Methods：Four hundred and fifty-six patients with coronary artery disease and type 2 diabetes were divided into percutaneous coronary intervention（PCI）（n=288）and coronary artery bypass grafting（CABG）（n=168）group. The primary endpoints were major adverse cardiac events（MACE, including all-cause deaths, myocardial infarction，stroke）within 12 months，and the second endpoint was target vessel revascularization within 12 months. Results：The rate of myocardial re-infarction in PCI group was higher than CABG group（P=0.014）within 12 months. There were no significant differences between two groups in total adverse cardiac events（P=0.072）and other MACE（all P>0.05）,also in target vessel revascularization（P=0.727）within 12 months. High-SYNTAX subgroup of PCI group had more incidence of adverse cardiac events than high-subgroup of CABG（P=0.017）,while there is no difference in low-subgroup and middle-one（all P>0.05）. Conclusions：It could not come to the conclusion that CABG was much better than PCI in patients with coronary artery disease and type 2 diabetes, but it might have lower incidence of adverse cardiac events in High-SYNTAX subgroup which patients accept CABG. Related Articles | Metrics

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The Clinical Significance of the Relationship of the Heart Valve Calcification and Carotid Intimamedia Thickness and Plaque Integral 2012, 40 (6):  612-614 .  Abstract ( 1556 )   PDF (331KB) ( 2164 )   Objective: To Research the Relationship of the degree of the heart valve calcification and carotid intimamedia thickness and plaque integral， and analyze its clinical significance. Methods : With transthoracic Doppler ultrasound imaging device ,the valve calcification of 206 patients were assessed, and all the patients were divided into four groups: no calcification group, mild calcification group, moderate group and severe calcification calcification group according to diagnostic criteria. The general information, medical history, blood pressure , blood lipids were Compared, simultaneous measured the intima-media thickness, soft plaque score, hard plaque score and the total plaque score of carotid artery of all the patients in the four groups. Results: The difference of age, gender, body mass index, smoking status, total cholesterol, triglycerides, high density lipoprotein, low density lipoprotein of the four groups was not statistically significant. The degree of valve calcification was positively correlated with coronary heart disease rate, systolic blood pressure , pulse pressure, carotid intima-media thickness, total plaque score and hard plaque score (r =0.546,0. 628,0. 512,0.802,0. 628,0.544, P<0.001) , and had no correlation with diastolic blood pressure and soft plaque score. Conclusion: Heart valve calcification can be a tool to speculate the carotid intima-media thickness, total plaque score and hard plaque score. Related Articles | Metrics

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The characteristics of serum Apelin and its relationship to glucose and lipid metabolism of essential hypertension 2012, 40 (6):  615-617 .  Abstract ( 1251 )   PDF (387KB) ( 2118 )   【Abstract】　Objective　To investigate the characteristics of Apelin in serum and its relationship to plasma lipid profil of essential hypertension(EH) and EH complicated with diabetes mellitus type 2(T2DM). Methods　34 patients with EH, 35 patients with EH and T2DM(EH+T2DM) and 19 healthy subjects of control group(NC) were selected, to measure the serum level of Apelin in each group by enzyme linked immunosorbent assay(ELISA)and also measure the totel cholesterol(TC)，triglyceride(TG)，high-density lipoprotein(HDL)， low-density lipoprotein(LDL)and fasting plasma glucose(FPG). Results The serum levels of Apelin in the group of EH and EH+T2DM were significantly higher than that in the group of NC(P<0.05)，the serum level of Apelin in the group of EH was significantly lower than that in the group of EH+T2DM (P<0.05).There were positive correlation between the serum apelin and systolic pressure(SP)，diastolic pressure(DP)，mean arterial pressure(MAP)，FPG，body mass index(BMI) and TG，and negative correlation between it and the HDL. Conclusion The serum level of Apelin in the group of EH may be high，and the serum level of Apelin is related with obesity，glucose and lipid metabolism. Related Articles | Metrics

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Clinical effect of preserving the parabiosis tissue of electrical burn wounds with Vacuum sealing drainage 2012, 40 (6):  618-620 .  Abstract ( 1429 )   PDF (804KB) ( 2177 )   Objective: To investigate the use of Vacuum sealing drainage (VSD) to preserve the parabiosis tissue of electrical burn wounds. Methods: We treated 30 clinical patients with a total of 42 electrical burn wounds with VSD, included 37 wounds use VSD directly after the surgical debridement; 5 wounds use VSD after surgical debridement and skin grafts. Result: Amount to 42 wounds were treated with VSD in this series, The skin grafts survived well and the wounds were completely healed. Discussion: Application of VSD in the treatment of post-debridement electrical burn wounds is conducive to the repair of these wounds. It can be an effective method to preserve the parabiosis tissue, promote skin grafts survived. Related Articles | Metrics

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The effect of different intensity of aerobic exercise on the level of serum visfatin in obese female adolescents Yingjia LIU 2,3 2012, 40 (6):  621-623 .  Abstract ( 1375 )   PDF (326KB) ( 2336 )   【Abstract】Objective To determine the effect of different intensity of aerobic exercise on the level of serum visfatin in obese female adolescents. Methods 120 obese female adolescents were enrolled in this study and then were randomly divided into control group and three aerobic exercise groups (30 subjects in each group). Subjects in aerobic exercise groups were given a 12-week training program of high, moderate and low intensity of aerobic exercise respectively, which included three 30-minute sessions per week. The levels of serum visfatin of the four groups before and after experiment were examined. Results No significant differences in the level of body mass index (BMI), homeostasis model assessment-insulin resistance (HOMA-IR) and serum visfatin before experiment were found between the four groups. After 12 weeks of aerobic exercise-training, the levels of BMI, HOMA-IR and serum visfatin were significantly decreased in all aerobic exercise groups. In addition, moderate intensity of aerobic exercise group showed higher reduction of these parameters compared with the other two groups. However, there were no significant differences in the levels of these parameters in control group before and after experiment. Conclusions Aerobic exercise, especially moderate intensity of aerobic exercise, can result in reduced level of serum visfatin in obese female adolescents. Related Articles | Metrics

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The clinical performance after mitral valve replacement with MOSAIC for mitral stenosis Yin YANG 2012, 40 (6):  623-624 .  Abstract ( 1476 )   PDF (305KB) ( 2293 )   Abstract Objective: to evaluate the left cardiac function and hemodynamic performance of Mosaic bioprosthesis in mitral valve replacement for mitral stenosis Methods: from January 2010 to December 2010,thirty-three patients underwent mitral valve replacement with Mosaic bioprothesis in hospital. Doppler echocardiograms were recorded before operation 、3 month and 1 year after valve replacement. Result：After operation，left atrium diameter (LAD)[（38.9±5.4）mm vs（51.7±8.7）mm]，left ventricular diastolic diameter(LVDD) [（49.4±5.8）mm vs（54.5±8.2）mm]，left ventricular systolic diameter(LVSD) [（33.0±5.0）mm vs（38.5±6.5）mm]， Pulmonary artery pressure (PAP) [（31.1±5.1）mmHg vs（44.3±8.1）mmHg] were significantly different between preoperation and postoperation at 1 year. Conclusions: The Mosaic bioprosthesis demonstrates excellent clinical performance after 1 year of follow-up. Related Articles | Metrics

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Epidemiological Research of Seven Kinds of the Respiratory Tract Virus Associated Acute Respiratory Tract Infections in Hospitalized Children of Tianjin 2012, 40 (6):  625-627 .  Abstract ( 1266 )   PDF (334KB) ( 2562 )   Abstract Objective: To understand the epidemiologic feature of virus infection in the children with acute respiratory tract infections in Tianjin area. Methods: From January 2010 to June 2011, 5954 hospitalized children with acute respiratory tract infection were studied. Nasopharyngeal aspirates were detected for seven kinds of the respiratory tract virus by direct immunofluorescent assay. Results: Viruses were identified in 626 patients, the positive ratio was 10.51 %, the majorty of them were under 2 years old. The most prevalent virus was respiratory syncytial viruses (RSV) (376, 60.06 %)，followed by parainfluenza 3 (211, 33.71 %), adenovirus (20, 3.19 %), influenza A (6, 0.96 %)，parainfluenza 1 (5, 0.80 %), influenza B (3, 0.48 %), parainfluenza 2 (2, 0.32 %) and coinfection (3, 0.48 %). RSV was maximum for the children between 0 to 12 years old. The seasonal change of RSV and PIV 3 was obvious. RSV was most detected in spring and winter, and the peak of PIV 3 appeared in summer. Conclusion: RSV and PIV 3 was the most common viruses in children with acute respiratory tract infections in Tianjin area, especially in children under 2 years old. Both RSV and PIV 3 had their own epidemiologic feature in different seasons. Related Articles | Metrics

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A Novel Mutation of Fibrillin-1 Gene Caused Serve Phenotypes of Familial Marfan Syndrome 2012, 40 (6):  627-627 .  Abstract ( 1538 )   PDF (566KB) ( 2178 )   Abstract Objiective: Marfan syndrome (MFS), an autosomal dominant inherited disease, has variable clinical manifestations typically affecting the cardiovascular, ocular and skeletal systems. Two causative genes for MFS have been demonstrated so far: Fibrillin-1 (FBN1) and TGFBR2 gene. This study aims to indentify the disease causing gene in a Chinese family with autosomal dominant MFS. Method:The detailed clinical examinations were performed and documented on each patient.DNA samples were collected from all participants.Exclusive mapping and mutation screening of FBN1 gene by direct sequencing were carried out. Results:After exclusion of the TGFBR2 locus by linkage analysis, significant linkage was found with markers of the FBN1 locus in the 15q21.1 region in both families. We then identified a novel mutation of FBN1,which is a premature termination codon (PTC) mutation. Conclusion:This server mutation correlates with the server phenotype, specifically the cardiovascular malformation, in the family. Related Articles | Metrics

临床论丛

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Percutaneous Nephrolithotomy with atmospheric pressure ballistic trajectory gravel treatment in the improvement Lateral Position: Report of 67 Cases 2012, 40 (6):  631-632 .  Abstract ( 1421 )   PDF (311KB) ( 2393 )   Abstract:Objective To evaluate the superiority and efficacy of percutaneous nephrolithotomy (PCNL) in the improvement lateral position.　 Methods A total of 67 patients were treated with PCNL in our hospital between July 2009 to February 2011. The patients were placed in the improvement lateral position(with Turning the lithotomy position 60 degrees to the lateral position. The Position of the cathter and then Pneumatic lithotripsy was used for stone fragmentation. The stone debris was removed by irrigation.　 Results Among the 64 cases, 51 were cured with the first operation, the other 13 received catheterization in the firstprocedure. None of the patients were converted to opensurgery. The mean operation time in this series was (132.4±34.1) min. The one stage cure rate in our patientswas 86.6% (58/67). None of the cases had massive blood loss, or injuries to the pleura and otherorgans. Follow-upwas available in 21 cases for1 to all cases have been taken off the water, 63 cases by a single channel, 4 cases by double channel, 11 cases treated by traditional Chinese medicine and ESWL had no residual stones Conclusions There are some advantages in elderly patients, surveillance, security, comfort and improve operation efficiency of the patients in the improvement lateral position.　 Related Articles | Metrics

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Clinical and neuroimaging features of posterior reversible encephalopathy syndrome 2012, 40 (6):  633-635 .  Abstract ( 1152 )   PDF (3437KB) ( 2301 )   Related Articles | Metrics