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15 December 2012, Volume 40 Issue 12 Previous Issue    Next Issue
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论著
实验研究
论著
药物临床观察
论著
实验研究
短篇论著
实验研究
短篇论著
临床论丛
短篇与病例报告
药物临床观察


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论著

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The relationship of blood glucose variability and microalbuminurla in type 2 diabetic patients in well-controlled glycosylated hemoglobin 2012, 40 (12):  0-0 .  Abstract ( 611 )   PDF (164KB) ( 2400 )   Objective To investigate the relationship of the blood glum variability and microalbuminuria(MAU)in type 2 diabetic patients with well-controlled glycosylated hemoglobin(HbAlc)。 Methods One hundred and thirsty-one type 2 diabetic patients with HbAlc under 7.0％ were divided high UAER group and normal UAER group。 Using the continuous glucose monitoring system (CGMS)，blood glucose fluctuate coefficient（BGFC），mean blood glucose（MBG）, percentage of time(PT) below 3.9mmol/L and above 7.8 and 11.1mmol/L were received。Then the data were analysis by the statistical analysis。Results (1)The course of disease ，uric acid （UA），fasting C-peptide, BGFC and PT(>7.8mmol/L) were significantly higher than those of the normal UAER group (p<0.05或p<0.01). (2)The course of disease ，urine albumin exclusion rate （UAER），serum glycosylated albumin（GA），MBG ，PT(>7.8mmol/L) and PT(>11.1mmol/L) in BGFC >1.5 group were higher than those of the BGFC<1.5 group (p= 0.016、0.022、0.001、<0.001、<0.001、<0.001）.（3）、Spearman regression analyses indicated that course of disease and UA and fasting C-peptide and BGFC were the independent influential factors of UAER（r=0.249，p=0.004；r=0.212，p=0.015 ；r=-0.199，p=0.023 ；r=0.191，p=0.029）. Logistic regression analyses indicated that UA and fasting C-peptide and BGFC were the risk factors of UAER (OR=1.349，p= 0.036；OR=1.005 p= 0.032；OR=1.582 p= 0.027) 。Conclusion In well-controlled patients with type 2 diabetes，course of disease and PT(>7.8mmol/L、>11.1mmol/L) and GA were the main factors influencing glucose variability。 The blood glucose variability is one of the risk factors for UAER。 Related Articles | Metrics

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The Expression of Livin and Caspase-3 in Laryngeal squamous cell Carcinoma and the correlation between Livin and Caspase-3 2012, 40 (12):  0-0 .  Abstract ( 410 )   PDF (211KB) ( 2019 )   PURPOSE: TO investigate the expression and their significance in laryngeal squamous cell carcinoma and the correlation between livin and Caspase-3. METHODS: Immunohistochemistry was used to detect the expression of livin and caspase-3 in 30 cases of polyp of vocal cord and 62 cases of laryngeal squamous cell carcinoma. RESULTS: The expression of livin laryngeal squamous cell carcinoma was significantly higher than that in polyp of vocal cord(P＜0.01), however the expression of Caspase-3 was significantly higher in polyp of vocal cord than that in laryngeal squamous cell carcinoma (P＜0.01). In laryngeal carcinoma,the expression of livin and Caspase-3 were associated with patients′Lymph node metastasis, Clinical stage and Differentiation (P＜0.05), but independent of Clinical classification ,smoking history,patients′age and sex(P＞0.05). A signifcantly negative relation was found between livin and Caspase-3 (P＜0.05). CONCLUSION: Livin was positively related to Caspase-3,which might play an important role in the carcinogenesis and development of laryngeal carcinoma by synergic effect. Related Articles | Metrics

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The Effect of Thyroid Hormones on Carotid Arteries Atherosclerosis in Type 2 Diabetic Subjects 2012, 40 (12):  0-0 .  Abstract ( 433 )   PDF (161KB) ( 1986 )   Abstract Objective: To explor The effect of thyroid hormone on carotid arteries atherosclerosis in type 2 diabetic subjects with euthyroidism。Methods:248 T2DM patients who hospitalized in Tianjin Metabolic Disease Hospital between January to December,2010 were divided into four groups : 92 patients (control group) without stenosis,32 patients (mild stenosis group) with stenosis<30%,93 patients (moderate stenosis group) with 30%≤stenosis<70%,31 patients (severe stenosis group) with stenosis>70% by color Doppler ultrasound of carotid arteries . The serum thyroid hormone were analysed in subjects of groups.Results: There were significant differences in the values of serum triiodothyronine(FT3)、high-density lipoprotein（HDL-C）between severe stenosis group and other three groups（P<0.01 or P<0.05）；Spearman’s correlation analysis demonstrated that carotid arteries atherosclerosis was negatively correlated with FT3、HDL-C（P<0.01）and positively correlated with body mass index(BMI)、glycosylated HbA1c、course of disease、age、low-density lipoprotein（LDL-C）、trglyceride（TG）、total cholesterol(TC)、systolic blood pressure（SBP）、diastolic blood pressure.Logistic regression analysis showed that age、SBP、TG heightening are independent risk factors affected the severity of carotid arteries atherosclerosis，FT3、HDL-C heightening are independent protective factors affected the severity of carotid arteries atherosclerosis.Conclusion: FT3 within a normal level might be a protect factor for atherosclerosis in patients with T2DM. Related Articles | Metrics

实验研究

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Construction of OCIL shRNA Expression construct and its Effect on RANKL/OPG Expression in Osteoblasts 1,ZHENG Fang 1 2012, 40 (12):  0-0 .  Abstract ( 443 )   Objective：To construct OCIL shRNA expression construct and to study its effects on RANKL/OPG expression in osteoblasts and on osteoclastogenesis. Methods：Small hairpin RNA (shRNA) coding sequences targeting rat OCIL mRNA were designed, synthesized and cloned into pRNAT-U6.1/Neo vector. The constructs were transfected into UMR106 cell line with lipofectamine 2000. The expression level of OCIL mRNA in the cells was determined by real-time quantitative RT-PCR. The expression levels of RANKL and OPG were also studied. The culture medium collected from the transfectant was used to treat rat bone marrow cells in the presence of PTH and the number of osteoclast like cells formed from marrow cells were scored. Results：The shRNA expression constructs significantly reduced OCIL mRNA in UMR106 cells after transfection. Moreover, the overexpression of OCIL shRNA resulted in a significant increase of RANKL mRNA and decrease of OPG mRNA. The culture medium collected from the transfectant promoted osteoclast-like cell formation from marrow cells in the presence of PTH. Conclusion：The inhibition of osteoclastogenesis by OCIL may involve the regulation of RANKL/OPG system. Related Articles | Metrics

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The association study between polymorphisms on TCF7L2、AKT2、FOXO1 gene and Type 2 Diabetes 2012, 40 (12):  0-0 .  Abstract ( 504 )   PDF (192KB) ( 1916 )   To investigate the relationship between the different gene polymorphism in transcription factor 7-Like 2(TCF7L2) , protein kinase B(AKT2) and forkhead box transcription factor subgroupO1 (FOXO1)gene of the WNT signalling pathway, and the type 2 diabetes mellitus(T2DM) in Tianjin Han populations. Methods: 352 T2DM patients and 176 case controls were randomly enrolled in this study. After the genome DNA was extracted, genotype frequency was detected using the denaturing high performance liquid chromatography. And different peaks were sequenced. Then the differences of different genotype frequency between the groups were analyzed, and risk factors of T2DM were evaluated using logistic regression. Results: It’s a good representation of the population used the Hardy-Weinberg equilibrium testing (P>0.05). In the T2DM group and case control group, the C/T genotype frequency of rs11669332 in AKT2 gene are 7.67% and 3.41%,there is no different between the two groups. The G/T genotype frequency of rs12255372 in TCF7L2 gene are 18.5% and 2.8% , The C/C and C/T genotype frequency of rs2701891 in FOXO1 gene are 3.1%、1.1% and 21.3%、10.2%,respectively.There are significant differences( P<0.01). Significant differences of the TCF7L2 T+/ FOXO1T+、TCF7L2 G+/ FOXO1 C+ genotypes frequency exists in the conjoint analysis between the two groups After a standard calibration of a(P<0.004).The TCF7L2 T+/ FOXO1C+ genotype, glycosylated hemoglobin, urea nitrogen are the risk factors of T2DM, high density lipoprotein is a protective factor. Conclusion: This study indicates that G/T genotype in the TCF7L2 and the C/C,C/T genotype in the FOXO1 gene significantly contribute to T2DM susceptibility in Tianjin Han populations. A combined effect could be found between TCF7L2 and FOXO1. These two polymorphisms are the risk factors of T2DM. Related Articles | Metrics

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Clinical Significance of Daniel ECG scores for earlier prognosis in acute nonmassive PTE ,yue-chuan LI 2012, 40 (12):  0-0 .  Abstract ( 444 )   PDF (196KB) ( 1969 )   Abstract Objective：To explore the clinical significance of Daniel ECG scores for earlier prognosis in acute nonmassive PTE.Methods：A total of 158 patients with acute nonmassive PTE ,confirmed by CTPA,were consecutively recruited from January of 2008 to September of 2011.The patients were divided into two groups according to Daniel ECG scores,including Group A(n=89),ECG score≥6 and Group B(n=69), ECG score<6.They were checked in 24-hour after being confirmed,including ECG,arterial blood gas analysis(ABG),serum markers and echocardiography. The ABG and echocardiography were rechecked after 14-day.Meanwhile,the clinic key things within 30-day in hospital were also checked.The connection of ECG scores,right ventricular function,respiratory function and the prognosis within 30-day in hospital were analysised.Results：There were statistical significant differences in arterial partial pressure of oxygen(PaO2), carbon dioxide(PaCO2),oxygenation index ,B-type natriuretic peptide(BNP),troponin I(TnI),right ventricular end-diastolic diameter(RVD),pulmonary artery inner diameter(PAD),tricuspid regurgitation velocity,and pulmonary artery systolic pressure(PASP) between Group A and B (P<0.05). There were statistical significant differences in sinus tachycardia,QRS axis>90°, SIQIIITIII,V5 lead R/S≤1,avR lead R>0.5mV and T wave inversion in chest lead among several ECG findings between Group A and B (P<0.05). At a cutoff of 6 points,the ECG score was 89.8% sensitive and 85.7% specific for the recognition of right ventricular dysfunction to nonmassive PTE .There were statistical significant differences in the hypocapnia and pulmonary hypertension(PASP≥50mmHg) within 30 days after therapy in hospital between Group A and B(P<0.01). Conclusion： Daniel ECG score is significant for patiens with acute nonmassive PTE to pretest the earlier prognosis. Related Articles | Metrics

药物临床观察

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The effect of intrathecal midazolam on anagesia efficacy of spinal anaesthesia in patients undergoing gynecologic surgery 2012, 40 (12):  0-0 .  Abstract ( 435 )   PDF (216KB) ( 1963 )   Abstract Objective ：To assess the subarachnoid block anesthesia effect of intrathecal bupivacaine and midazolam on patients undergoing gynecologic operation. Methods： A total of 86 patients scheduled for elective myomectomy procedures were selected to participate in this study. According to random number table, patients were allocated into two groups and each group was 43 cases. Group A received 3 mL 0.5% bupivacaine with 0.4mL saline, and group B received 3 mL 0.5 % bupivacaine and 0.4 mL (2mg) midazolam mixture. The onset, duration of sensory/motor block, sedation score and side effects were observed in the experiment. Results： Compared with group A, the duration of sensory blockade was prolonged in the group B(midazolam group), p<0.05, while the time and duration of motor blockade and sedation score were comparable. However he rate of nausea and vomiting has significant difference(p<0.05).Conclusion： Intrathecal bupivacaine with 2mg midazolam resulted in prolonged postoperative analgesia and decreased the adverse effect without increasing motor block time. Key words：analgesia; bupivacaine; intrathecal injection; midazolam Related Articles | Metrics

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Expression of MicroRNA-93 in Osteosarcoma and the Significance 2012, 40 (12):  0-0 .  Abstract ( 499 )   PDF (210KB) ( 1915 )   Objective: To analyse the expression of microRNA-93 (miR-93) in osteosarcoma and investigate the effect of miR-93 on the proliferation and cell cycle in osteosarcoma cell lines. Methods: To detect the expression of miR-93 in 38 pairs of osteosarcoma and cartilaginous tissues using TagMan MGB Real-time PCR. To construct the miR-93 overexpressed vector and synthesis the miR-93 antisense nucleotide sequence. Then they were transfected into osteosarcoma cell lines U2-OS and MG-63. Cell proliferation was detected by MTT assay and cell cycle was detected by flow cytometry. Results: 65.8%（25/38）of miR-93 was overexpressed in osteosarcoma compared to cartilaginous tissue (P<0.05). Overexpression of miR-93 could promote the growth of osteosarcoma cell lines and delay the cell cycle in S phase. The reduction of miR-93 could inhibit the growth of osteosarcoma cell lines and delay the cell cycle in G0/G1 phase. Conclusions: miR-93 was overexpressed in osteosarcoma and miR-93 could regulate the growth of osteosarcoma cells via mediating the transition of G1/S phase, which may become a targeted gene of osteosarcoma. Related Articles | Metrics

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Clinical Significance of the Difference Expressions of Heparanase in Glomerulus of IgA Nephropathy 2012, 40 (12):  0-0 .  Abstract ( 341 )   Objective:To explore the expression of heparanase in glomerulus of patients with IgA nephropathy and its relationship with clinical parameters. Methods:A total of 78 patients with IgA nephropathy diagnosed by biopsy were classified into 4 groups according to the Lees classification. Comparing the clinical parameters in patients of five groups including urea nitrogen, serum creatinine, 24h proteinuria quantitation and complement in urine and hematuria. Immunohistochemistry was applied to detect the expression of Hpa in glomerulus of patients with IgAN and control group. Results:Heparanase expressed at a very low level in the glomerulus in control group.Compared with control group, the expressions of heparanase were significantly increased in glomerulus of IgAN groups (P<0.05). There was significant difference of the expressions of heparanase in 4 groups (P<0.01). The expressions of heparanase positively correlated with the 24h proteinuria quantitation (P<0.01); moreover the expressions of Hpa did not correlate with serum creatinine, urea nitrogen, complement in urine and hematuria (P>0.05). Conclusion:The abnormal expressions of heparanase may correlate with the renal pathology and the development of proteinuria in patients with IgAN ，namely, down- regulation of heparanase may be good for the recovery of IgAN. Related Articles | Metrics

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The association between HIF-1α and iNOS expression and Helicobacter pylori infection in gastric adenocarcinoma 2012, 40 (12):  0-0 .  Abstract ( 514 )   PDF (171KB) ( 1956 )   Abstract Objective: To investigate the expression of HIF-1α and iNOS in Helicobacter pylori (Hp) -positively gastric adenocarcinoma (GAC). Methods: we collected 198 paraffin-embedded human GAC samples which have detailed follow-up and clinicopathologic data , meanwhile the 98 gastritis samples were collected as control group which divided into atrophic gastritis group and non-atrophic gastritis group.All of them have been accepted as c14 breath test to identify Hp infectious atatus. Using immunohistochemical staining to detect the expression of HIF-1a and iNOS. Results: Hp-positive infection was most frequently in GAC group than that of atrophic gastritis group and non- atrophic gastritis group (69.70%、59.62%和47.83%). The more positive expression of HIF-1a and iNOS can be seen in GAC group than that of atrophic gastritis group and non-atrophic gastritis group（P＜0．05）. The positive expression of HIF-1a and iNOS were higher in Hp+ GAC than in Hp- GAC.Conclusion: The carcinogenesis of Hp might be through the pathway of HIF-1a and iNOS. Related Articles | Metrics

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The study of correlation between the Mycoplasma pneumonia infection and acute exacerbation of chronic obstructive pulmonary disease hospitalization ratio 2012, 40 (12):  0-0 .  Abstract ( 423 )   [Abstract] Background and study objective: The aetiology of acute exacerbations of chronic obstructive pulmonary disease (COPD) is heterogeneous and still under discussion. Serological studies have suggested that Mycoplasma pneumoniae(Mp) may play a role in acute exacerbations of COPD（AECOPD）. We want to explore the correlation between the Mp infection and AECOPD hospitalizations ratio. Methods : The presence of these Mp in Serological samples was investigated in patients with AECOPD and other kinds of respiratory infection patients using passive agglutination. The present study was retrospective study and a total of 418 serological samples from 298 AECOPD inpatients (study group) and 120 other kinds of respiratory infection patients (control group) were included. According to the lung functions of the AECOPD patients the study group were divided into four subgroup. By the results of the Mp investigation the study group was divided into Mp positive group and Mp negative group. Results: Mp seroprevalences of the study group (22.82%) were higher than in the control group (12.5%) with statistical significance (P<0.01). The seroprevalences of the sub-study group were 19.28%, 25.81％,23.63％,23.52％ respectively but without statistical significance(P>0.05).We also found that the seroprevalences was different as the time go on and also there was no correlation between AECOPD hospitalization and Mp Seroprevalences（r=0.305，P > 0.05）. There were no significant differences between these two groups divided by the Mp value in a broad spectrum of patient- and exacerbation-related clinical variables. Conclusion : We have shown that microorganisms causing acute exacerbations of COPD are not only typical bacteria but also atypical pathogens with unpredictable high rates. The seroprevalences was related with outbreak time not the severity of the disease. Pay more attention to the Mp maybe get better prognosis for most patients. Related Articles | Metrics

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The Study of Fiberoptic Duct Endoscopy (FDS) Iodine Staining for the Diagnosis of Intraductal proliferative Lesions 2012, 40 (12):  0-0 .  Abstract ( 482 )   Background Lugol’s solution has been successfully applied to esophagus, cervix and oral cavity in order to detect lesion in early carcinoma and precancerous disease. The objective of this thesis is to probe into the value of fiberoptic duct endoscopy (FDS) iodine staining for the diagnosis of intraductal proliferative lesions. Methods Periodic acid Schiff (PAS) staining was first of all to be implemented on fifty-five cases of mammary gland hyperplasia tissue and 57 cases of Invasive breast cancer tissue; In vitro iodine staining was then to be effected on 64 cases of fresh mammary gland hyperplasia tissue and 53 cases of invasive breast cancer tissue; what follows was to select 177 cases of patients with nipple discharge on whom FDS was to be carried out; of the 177 patients 92 were chosen by random and on them endoscopic Lugol's iodine staining should be effected, with the other 85 serving as the control group, whose suspicious lesions should be cut and inspected with wire positioning, thus evaluating the value and clinical significance of Lugol's iodine staining for the diagnosis of intraductal proliferative lesions. Results PAS stainings were positive in mammary gland hyperplasia while PAS positive substance would be gradually eliminated until an neglectable level（P<0.05）, that Mammary gland hyperplasia specimens took on a color of tan brown after supravital staining whereas the color of breast cancer samples after staining got significantly lighter or unstained, and that the detection rate of ductal intraepithelial neoplasia ( DIN )by means of fiberoptic duct endoscopy (FDS) iodine staining was 93.0% and the rate among the control group 70.9%（P<0.05）. Conclusion The detection rate of DIN can be boosted by means of endoscopic breast ductal epithelial iodine staining and this approach deserves to be applied and extended. Related Articles | Metrics

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The Effect of Telmisartan on Rho / ROCK Signal Pathway in Insulin Resistance Rat’s Coronary Artery 2012, 40 (12):  0-0 .  Abstract ( 424 )   PDF (198KB) ( 1987 )   Objective:To observe the express level change of RhoA and ROCK signal pathway in insulin resistence Rat and To identify the role of telmisartan on the expression of RhoA and ROCK signal pathway in insulin resistence Rat small cornary artery tissue. Methods:There were 30 male Sprague-Dawley (SD) Rats was randomly divided into 3 groups,10 in each group.The control group was feed with normal diet ,10 in Insulin resistance group (creat model by high fructose diet plus Streptozotocin（25mg/Kg） injection ),10 in telmisatan group (insulin resistance plus telmisartan5mg/kg/d). 8 weeks later,Coronary arteries were isolated for the RT-PCR and Westen Blot assay to identify RhoA,ROCK1,ROCK2 mRNA and proten expression level.Results : Compared with control group, RhoA proten express level down regulated but ROCK1mRNA express level was up regulated in IR group. After interfere by telmisartan , RhoA proten expression was remarkably higher than IR group (P=0.043) .there were no statistical significance of RhoA,ROCK1mRNA and ROCK1,ROCK2 proten expression between Tel and IR group .Conclusions:Telmisartan can reverse the RhoA protein down regulation in IR rat’s coronary artery ,but only have a limited affect on RhoA mRNA ,ROCK1 and ROCK2mRNA and protein expression level in insulin resistance rat’s coronary artery. Related Articles | Metrics

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The Role of Bax in the 14-3-3γ Protection against Cardiomyocytes Damage Induced by LPS 2012, 40 (12):  0-0 .  Abstract ( 421 )   Abstract Objective: To investigate the exogenous gene 14-3-3γ against cardiomyocyte damage induced by LPS, and explore the role of Bax of Bcl-2 family involving in the process. Methods: Neonatal SD rat cardiomyocytes were cultivated and constructed recombinant plasmid of pFLAG-14-3-3γ were transfected into the cells, which were treated with LPS subsequently. After treatment, 14-3-3γ levels were detected by western blotting, cells viability was analyzed by MTT, the activity of LDH and CPK were determined by auto-biochemistry analysator, the levels of Bax protein in cytoplasm and mitochondria were detected by western blotting. Results: 14-3-3γ protein significantly increased in the cardiomyocytes transfected by pFLAG-14-3-3γ. Compared with control group, cardiomyocytes treated with LPS had significantly reduced cells viability(P<0.01), increased LDH and CPK activities(P<0.01), and promoted Bax translocation from cytoplasm to mitochondria. Elevated l4-3-3γ protein resulted by transfection of pFLAG-14-3-3γ reversed cardiomyocytes damage induced by LPS. Compared with untransfected group, cardiomyocytes of transfected group had significantly increased cells viability(P<0.01), decreased LDH and CPK activities(P<0.01), and inhibited Bax translocation from cytoplasm to mitochondria. Conclusion: 14-3-3γ protects cardiomyocytes against LPS damage by inhibiting Bax translocation from cytoplasm to mitochondria. Related Articles | Metrics

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Study on the Protective Effects of hirudo to arterial endothelium Gui-Wen LIANG1, 2012, 40 (12):  0-0 .  Abstract ( 464 )   PDF (176KB) ( 1858 )   Objective: atherosclerosis model in rabbits were established by feeding high-fat diets. to observed the effects on protect the vascular endothelium of hirudo and atorvastatin. Methods: feeding group A (normal diets) and model group (high-fat diets) 9 weeks, after the successful establishment of models divided the alive rabbits into four groups: rabbits in group B were fed with high-fat diets; rabbits in group C were fed with high-fat diets which mixed hirudo medicinalis powder 0.5 g/Kg/d; rabbits in group D were fed with high-fat diets which mixed hirudo medicinalis powder 1.5g/Kg/d; rabbits in group E were fed with high-fat diets which mixed atorvastatin 2.5mg/Kg/d. At the end of 17th week, blood samples were taken and made into serum for testing assay lipid , NO, ET-1, PGF-1-α, TXB2, and CRP. Then all rabbits were sacrificed, and the thoracic aortas of them were taken out. The arteries were embedded in paraffin and cut into transverse sections. They were subjected to the HE-staining which could observe changes of the arteries in morphological. Results: Macroscopic observation：there was no difference of the plaque area between group D and group E, and both smaller than group B(p＜0.05). The TC, LDL, TXB2, ET-1 and CRP of group D higher than group B, while the NO and PGF-1-α lower than group B. The TC, LDL and NO of group D higher than group B while the ET-1 lower than group B. All the differences had statistical significance. Discussion: Hirudo has a remarkable effect on protect the vascular endothelium . Its mechanism is related to increasing NO and PGI2, while decreasing TXB2 and ET-1. Related Articles | Metrics

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Effects of hemoperfusion on cholinesterase activity and inflammatory mediators in patients with severe acute organic phosphorus poisoning 2012, 40 (12):  0-0 .  Abstract ( 363 )   PDF (162KB) ( 2029 )   Abstract Objective To investigate effects of hemoperfusion on cholinesterase activity and inflammatory mediators in patients with severe acute organic phosphorus poisoning. Methods Cholinesterase activity, recovering time of cholinesterase, APACHEⅡ scores, inflammatory mediators, hospitalization time, cumulative dosage of atropine, mortality rate were measured in 55 patients treated with routine treatment (control group) and 55 patients treatd with hemoperfusion based on routine treatment (hemoperfusion group). Results Compared with control group, , APACHEⅡ scores, recovering time of cholinesterase, hospitalization time, cumulative dosage of atropine, mortality rate in hemoperfusion group were significantly reduced(P all <0.05), while cholinesterase activity in hemoperfusion group were significantly increased in the third day after hemoperfusion treatment (P<0.05). Serum tumor necrosis factor-α( TNF-α), interleukin-6, high-sensitivity C-reactive protein (hs-CRP) of patients in hemoperfusion group were significantly reduced after 3 days with hemoperfusion (P all <0.01). Conclusions Early hemoperfusion may improve prognosis via reducing inflammatory mediators in patients with severe acute organic phosphorus poisoning. Related Articles | Metrics

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Effect of valsartan, atorvastatin and pioglitazone on the expression of adrenomedullin and complement factor H in mesangial cells cultured under high glucose condition Ji-Juan ZHONG 2012, 40 (12):  0-0 .  Abstract ( 426 )   PDF (512KB) ( 2016 )   Objective: To observe the effect of valsartan, atorvastatin and pioglitazone on the expression of TGF-β1, extracellular matrix, adrenomedullin and complement factor H in mesangial cells cultured under high glucose levels and to investigate its mechanism. Methods: The experiment was divided into low glucose group(LG), high glucose group (HG), high glucose+valsartan(HD), high glucose+atorvastatin (HL)and high glucose+pioglitazone(HP) . ELISA and RIA were used to measure the content of TGF-β1，laminin and type Ⅳ collagenin of the cultured mesangial cells under high glucose conditions. RT-PCR was applied to determine the expression of TGF-β1mRNA, AMmRNA and CfhmRNA. Results: Valsartan, atorvastatin and pioglitazone could significantly decrease the concentrations of TGF-β1，laminin and type Ⅳ collagen in the supernatants for a certain extent compared with HG control group（P<0.01）,and they could also inhibit the expression of TGF-β1mRNA, AMmRNA and CfhmRNA of the cultured mesangial cells under high glucose conditions（P<0.01）. Conclusions: Valsartan, atorvastatin and pioglitazone showed a certain renoprotective functions which might relates to the inhibition of over-expression of TGF-β1 and extracellular matrix in mesangial cells induced by high glucose. Mean while, the expression of AM and Cfh was reduced, which might also relates to the variation of TGF-β1. Related Articles | Metrics

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A Study of Hypercoagulation State in Nephrotic Syndrome of Pregnancy 2012, 40 (12):  0-0 .  Abstract ( 385 )   PDF (147KB) ( 1871 )   Abstract：Objective To study the hypercoagulation state in nephrotic syndrome of pregnancy . Methods Coagulation and hemorrheology indexes were tested and analyzed contrastivly between 63 patients of nephrotic syndrome of pregnancy and 201 patients of severe pre-eclampsia. Results Compared with patients of severe pre-eclampsia,prothrombin time（PT）and activated partialthromboplastin time（APTT）were longer,D-dimer (D-D)and fibrinogen (FIB)were lower and antithrombin Ⅲ(AT-Ⅲ) increased,and blood viscosity(ηb)、plasma specific viscosity (ηp)、whole blood reduced viscosity (ηr)、hematocrit (HCT)、erythrocyte aggregation index (VAI)and thrombosis index(TFL)increased，erythrocyte deformability index(DI) decreased in patients of nephrotic syndrome of pregnancy.The differents were significant（P<0.05）.Conclusion The hypercoagulation state of nephrotic syndrome of pregnancy was more severer than commonly pre-eclampsia. Related Articles | Metrics

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Analysis of Pancerebral Angiography of Ischemic Cerebrovascular Disease with Type Ⅱ Diabetes 2012, 40 (12):  0-0 .  Abstract ( 390 )   PDF (166KB) ( 1863 )   【ABSTRACT】 Objective::To investigate the characteristics of pancerebral angiography of ischemic cerebrovascular disease with type Ⅱ diabetes. Method:Compare the results of the pancerebral angiography of 247 cases of ischemic cerebrovascular disease with type Ⅱ diabetes(the diabetes group) and 372 cases of ischemic cerebrovascular disease without type Ⅱ diabetes(the control group) .Result:①The difference of vascular stenosis of the two groups has statistical significance(x2=31.57,p<0.05).There are more cases of moderate-severe vascular stenosis and occlusion in the diabetes group than the control group(x2=30.34,p<0.05). ②The majority of vascular involvement are intracranial blood vessels in the two groups. The middle cerebral arteries are the most easily involved. But the difference is not significant(x2=0.02, p<0.05).③Multiple blood vessels are involved for the majority of the two groups. But the incidence rate is higher in the diabetes group than the control group (72.05%:53.56%).The majority of vascular involvement in the diabetes group were diffuse without collateral circulation (64.63% and71.18%). The majority of vascular involvement in the control group are not diffuse without collateral circulation. Conclusion: The characteristics of pancerebral angiography of ischemic cerebrovascular disease with type Ⅱ diabetes: vascular stenosis is more severe and the majority of vascular involvement are intracranial arteries with multiple, diffuse and little compensatory manfestations. Related Articles | Metrics

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The relationship between CXCL5 gene polymorphisms -156G/C (rs352046) and diabetes mellitus in Han minority population in hebei province. 2012, 40 (12):  0-0 .  Abstract ( 469 )   PDF (148KB) ( 1898 )   【Abstract】Objective To investigate the relationship between the CXCL5 gene –156G/C polymorphism and type 2 diabetes mellitus in Han population of Qinhuangdao region of north China's Hebei Province. Methods The polymorphism of CXCL5 gene promoter region -156G/C was detected by PCR-RFLP method in 210 patients with type 2 diabetes and 171 normal controls. The genotype and allele frequencies distributions were compared between two groups. Results Distribution of genotype and allele of the two groups are not statistically significant（χ2=2.80， p=0.247；χ2=1.029， P=0.310）. The –156G/C polymorphism was not associated with type 2 diabetes，（OR=1.214 95%CI= 0.859-1.716）. Conclusions In Qinhuangdao Area, T2DM group and the control group show no statistically significant difference in the distribution of genotype and allele. Perhaps, CXCL5 genetic polymorphism is not a risk factor for diabetes in Hebei Han minority. Related Articles | Metrics

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Effects of dexmedetomidine in inhibition of cardiovascular response to double-lumen endobronchial intubation 2012, 40 (12):  0-0 .  Abstract ( 410 )   PDF (244KB) ( 1907 )   Objective To observe the dexmedetomidine in inhibition of cardiovascular response to double-lumen endobronchial intubation. Methods Thirty ASA I ~ II patients, aged 18-65 years, weighing 50-80kg, scheduled for lung or esophageal surgery were randomly assigned to two groups: control group (C group, n=15) and dexmedetomidine group (D group, n=15), group D was given dexmedetomidine 1μg/kg before anesthesia induction, infusion time was 15min; group C was given 10ml saline instead of dexmedetomidine. BP and HR were recorded before infuse of dexmedetomidine(T0), before intubation (T1), immediately after intubation (T2) and at 1,3,5,10 min after intubation (T3-T6). Results Compare with C group, HR was significantly decreased at T1 in group D(P <0.05); HR, SBP and DBP were lower at T2-T5 in group D (P <0.05); HR, SBP and DBP were no difference at T6 in group D (P>0.05). Conclusion 1μg/kg dexmedetomidine infusion before anesthesia may effectively inhibit double-lumen endotracheal tube intubation cardiovascular responses. Related Articles | Metrics

临床论丛

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Investigation of endourlogical operation in treating BPH and bladder calculus jian jun wu 2012, 40 (12):  0-0 .  Abstract ( 427 )   PDF (163KB) ( 1936 )   Objective: To evaluate the feasibility of transurethral resection of the prostate combined with Ho:YAG laser or lithotrite lithotripsy of bladder stones for treatment of patients with bladder stones and BPH. Then to compare the therapeutic effects of the two methods. Methods: A single-blind, randomized controlled trial was adopted. The clinical data of 43 cases of patients with bladder stones and BPH were retrospectively reviewed . including 20 cases treated with TURP and lithotrite and 23 cases treated with TURP and Ho:YAG laser. The perioperative indicators before operation or after operation were compared in the two groups. Results: All patients this team rehabilitated smoothly, no complications were observed ,No TUR syndrome，or residual stone occurred during and after the operations. Maximum urinary flow rate was apparently increased. There were remarkable differences in lithotripsy success rate between two groups (P<0.05)．There were also remarkable differences between in lithotripsy success rate and efficiency between two groups, when the diameter is move than 2cm. The therapeutic effects of the two lithotripsies is the same when the diameter of the bladder calculus is less than 2cm . when the diameter is more than 2cm,Ho:YAG laser lithotripsy is more effective than lithotrite．Conclusion: Our data indicate that simultaneous TURP and Transurethral Ho:YAG laser lithotripsy of bladder stones do not increase the complication, and is safe and effective alternative treatment. Related Articles | Metrics

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Analysis of different approaches in treating middle and distal femoral fracture 2012, 40 (12):  0-0 .  Abstract ( 522 )   PDF (1KB) ( 1821 )   Objective：Analyse different approaches in treating middle and distal femoral fracture in order to provide best approach. Methods：Use statistical methods to analyse different approaches in operation time, bleeding amount, postoperative range of motion(ROM) in knee in two groups. Results：There is significant difference in operation time, bleeding amount, postoperative ROM in knee in two groups .Anterolateral approach has more advantage. Conclusion：Anterolateral approach in treating middle and distal femoral fracture is better than lateral approach ,which has shorter operation time, less bleeding amount, lower infection rate and larger postoperative ROM in knee . Related Articles | Metrics

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The safety and efficacy comparison of intravenous propafenone and loading oral propafenone for cardioversion of atrial fibrillation 2012, 40 (12):  0-0 .  Abstract ( 420 )   PDF (185KB) ( 2111 )   The safety and efficacy comparison of intravenous propafenone and loading oral propafenone for cardioversion of atrial fibrillation Abstract Object To monitor Widely used the clinical observation of intravenous propafenone for cardioversion of atrial fibrillation in elderly patients, whether it is safe out of hospital serving loading oral propafenone prescription cardioversion of atrial fibrillation. Method Clinical method selected> 60 years paroxysmal atrial fibrillation were randomly divided into two groups: intravenous loading dose of propafenone and oral propafenone loading dose group, patients with successful cardioversion hospital serving loading oral propafenone therapy out hospital . Prescription were compared cardioversion rate, incidence of side effects, the rate of emergency department visits. The results of the tow group first serving loading oral propafenone side effects (8.9% vs5.7%, p> 0.05), no statistically significant difference, while the out of hostpital treatment of side effects of propafenone loading rate (8.9% vs1.25%, p <0.05 ), emergency treatment rate (19.0% vs5%, p <0.001), according to statistically significant. Patients with atrial fibrillation in the first turn, should hospital oral loading dose, side effects can be timely manner to ensure patient safety, even if we can tolerate intravenous bolus propafenone cardioversion o f atrial fibrillation, it can not predict the loading oral service propafenone adverse reactions. Related Articles | Metrics

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Clinical Features and Countermeasures of Ectopic Pregnancy caused by Assisted Reproductive Technology 2012, 40 (12):  0-0 .  Abstract ( 445 )   Abstract Objective: To investigate the clinical characteristics and countermeasures of ectopic pregnancy (EP) after assisted reproductive technology (ART), deepen the understanding of complications of ART and reduce the misdiagnosis or missed diagnosis of EP after ART. Methods: The types, high risk factors and surgical treatment options were studied in 49 cases of EP after ART in Assisted Reproduction Center of our hospital during the past 12 years. Results: The tubal pregnancy is the most common type of EP after ART. The simple tubal pregnancy and tubal pregnancy in heterotopic pregnancy (HP) cases account for about 85.71% of the total number. Other types included cornual pregnancy, ovarian pregnancy and abdominal pregnancy. The high risk factors of EP after ART included tubal factors, endometriosis, EP surgery and other gynecological surgery history, abortion history, uterine factors. And the difference of above high risk factors was significantly significant (X2 = 34.8, P <0.001). Tubal factors were accounted for a percentage of 42.86%, which was the main factor for EP after ART. Patients with tubal pregnancy were performed laparoscopic ipsilateral or bilateral salpingectomy. Patients with cornual pregnancy, based on fetal sac location, were performed either aspiration of uterus by laparoscopy combined with abdominal ultrasound or laparoscopic cornual patial resection. There were no persistent ectopic pregnancy in 49 postoperative patients. The clinical cure rate was 100%. Conclusion: The tubal factor is one of the most important risk factors induced EP after ART. The salpingectomy is recommended before ART for infertility patients caused by tubal factors to reduce the probability of ectopic pregnancy. Doctors should communicate with patients before ART and make them mentally prepared, in order to promote early detection and timely treatment for EP. Patients should have surgical treatment immediately to remove the EP lesions, and also strive to continue the intrauterine pregnancy. Related Articles | Metrics

短篇与病例报告

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one case of diabetes combined with autoimmune hypophysitis 2012, 40 (12):  0-0 .  Abstract ( 323 )   PDF (296KB) ( 2000 )   Related Articles | Metrics

药物临床观察

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Effects of cisatracurium at room temperature for different time on the course of neuromuscular block 2 2012, 40 (12):  0-0 .  Abstract ( 375 )   PDF (141KB) ( 1944 )   Abstract Objective: Compare the effects of cisatracurium at room temperature on the course of neuromuscular blockade. Methods Sixty patients undergoing elective abdominal surgery with general anesthesia were randomly assigned to one of four groups (n=15 each). In group Ⅰ, cisatracurium was kept in room temperature(22~24℃）for 4 hours.In group Ⅱ, cisatracurium was diluted two times with 0.9%sodium chloride，then it was kept in room temperature for 4 hours.In group Ⅲ, cisatracurium was kept in room temperature for 12 hours.In group Ⅳ（control group）, cisatracurium was kept in fridge at 2~8℃ before using. Anesthesia was induced with midazolam 0.05mg/kg, fentanyl 1~2μg/kg and propofol 1.5~2mg/kg intravenously. Laryngeal mask was inserted after cisatracurium 0.15 mg/ kg . Anesthesia was maintained with propofol 6~10mg/（kg．h）. Neuromuscular function was assessed by accelerography. TOF stimulation of ulnar nerve was used. The onset time,recovery of first twitch(T1) and recovery of TOF 25% were recorded. Results There was no significant difference in onset time,recovery of first twitch and recovery of TOF 25% between group Ⅰ and control group. In group Ⅱ and group Ⅲ, recovery of first twitch(T1) and recovery of T4/T1 25% were significantly shoter than control group and group Ⅰrespectively. Conclusion Cisatracurium was kept in room temperature for 4h,which has little effects on the course of neuromuscular blockade. However, the clinical duration of cisatracurium was siginificantly shorter when it was kept in room temperature for 12h or when it was diluted two times with 0.9%sodium chloride and then kept in room temperature for 4h. Related Articles | Metrics

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Observation of the Clinical efficacy of Kushen Injection Combined with Concurrent Chemoradiotherapy in Locally Advanced Nasopharyngeal Carcinoma Clinical 2012, 40 (12):  0-0 .  Abstract ( 397 )   PDF (180KB) ( 2080 )   Abstract Objective To observe the recent adjuvant clinical efficacy and side effects of Compound kushen injection in treating with locally advanced nasopharyngeal carcinoma patients undergoing concurrent chemoradiotherapy. Methods 120 patients with locally advanced nasopharyngeal carcinoma for initial treatment in our hospital from October, 2006 to November, 2011 were randomly divided into two groups: the kushen group (n=60) and the control group (n=60). Both groups were treated with concurrent chemoradiotherapy, and the kushen group was given compound kushen injection in addition. Results There were no significant differences (P>0.05) between the complete response rates in the kushen group and the control group. The side effects in the kushen group were significantly lower than those in the control group, and the quality of life in the kushen group were better than that in the control group (P <0.05) Conclusion The compound kushen injection could not improve the clinical efficacy, but it could alleviate the side effects caused by concurrent chemoradiotherapy so as to improve the quality of life and to facilitate completing chemoradiotherapy. Related Articles | Metrics