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15 February 2013, Volume 41 Issue 2 Previous Issue    Next Issue
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论著
实验研究
短篇论著
药物临床观察
临床论丛


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论著

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The Significance of Serum Thymosin β4 Levels in Patients with Non-alcoholic Fatty Liver Disease 2013, 41 (2):  97-100 .  Abstract ( 495 )   PDF (465KB) ( 2320 )   [Abstract] Objective  To investigate serum thymosin β4 (Tβ4) levels in patients with non-alcoholic fatty liver disease (NAFLD)，and to explore the correlation between Tβ4 and serum biochemical parameters. Methods The serum Tβ4 level and biochemical parameters were detected in 40 putients with NAFLD. In addition, the levels of serum Tβ4 and biochemical parameters were detected before and after treatment with silibinin or polyene phosphatidylcholine in 26 NAFLD patients. The receiver operator characteristic (ROC) analyses for ALT and Tβ4 level were performed and cut-off value was determined. Results  Compared with the healthy control group, serum Tβ4 levels were significantly lower in patients with NAFLD (P＜ 0.01). There was a negative correlation between serum Tβ4 and ALT in patients with NAFLD（r=-0.330，P＜0.05). The serum level of Tβ4 was significantly increased after treatment in 26 NAFLD patients (P＜0.01). There were significant differences in levels of TG，ALT，ASTm，GGT，TBIL，ALP，ALB and FPG between NAFLD group and healthy control group (P＜0.05 or P＜ 0.01). After treatment, the levels of TG，ALT，ASTm，GGT，TBIL，ALP，ALB and FPG improved in patients with NAFLD (P＜ 0.05 or P＜0.01). AUC of serum Tβ4 was 0.906 with 4.830 5 mg /L cut off value. Its sensitivity was 85% and specificity was 80%, which were higher than those of ALT. Conclusion  The serum levels of Tβ4 were significantly lower in NAFLD patients than those of healthy control. And the level of serum Tβ4 was significantly increased after treatment in NAFLD patients. The serum level of Tβ4 was a more sensitive index than ALT for patients with NAFLD. Related Articles | Metrics

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Study on the Relationship between levels of 2h-PG and changes of Islet β-cell function and Blood Lipid in Obese Children 2013, 41 (2):  101-104 .  Abstract ( 800 )   PDF (2544KB) ( 2320 )   [Abstract] Objective  To investigate the relationship between the 2 h plasma glucose level after oral glucose tolerance tests (2h-PG) and islet β-cell function and blood lipid in obese children. Methods  A total of 102 obese children aged 9-15 years were enrolled in this trial. Levels of fasting plasma glucose (FPG) in children were all lower than 5.6 mmol/L. According to 2 h-PG, subjects were divided into G1 (2 h-PG≤5.6 mmol/L) group，G2 (5.7~7.7 mmol/L) group and G3 (2 h-PG ≥7.8 mmol /L) group．The parameters related to glucose and lipid metabolism were compared between groups. The relationship between 2 h-PG and all indexes were analyzed. Results  Levels of glycosylated hemoglobin（HbA1c) and FPG were significantly lower in G1 group than those in G2 group and G3 group. Levels of fasting insulin (FINS), 0.5 insulin (0.5 h-INS) and insulin resistance index (HOMA-IR) were significantly higher in G3 group than those in G1 and G2 groups. The level of high-density lipoprotein cholesterol（HDL-c）was significantly lower in G3 group than that in G1 group. There were significant differences in 0.5 plasma glucose after oral glucose tolerance tests (0.5 h-PG), 2-hour insulin after oral glucose tolerance tests（2h-INS), low density lipoprotein cholesterol (LDL-c) and early insulin secretion index corrected by insulin resistance (IR-EIS) between three groups (P < 0.05 or P < 0.01). The level of 2 h-PG was positively related with HAb1c，FPG， 0.5 h-PG，0.5 h-INS，2 h-INS，HOMA-IR and LDL-c (P < 0.05 or P < 0.01)，but 2 h-PG was negatively related with IR-EIS (P = 0.002) after deduction of the effects of age and BMI. Multiple linear regression analysis showed that 2h-INS， FPG，FINS and IR-EIS were influencing factors of 2 h-PG. Conclusion  When 2 h-PG is higher than 5.6 mmol/L in obese children，there has been early islet β-cell function decline and dyslipidemias. Related Articles | Metrics

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The Inhibitory Activity of a Novel Small-Molecule Inhibitor of IL-6 Signalling in vitro FU Xue jiao,CHEN Jian feng 2013, 41 (2):  105-109 .  Abstract ( 493 )   PDF (3512KB) ( 2322 )   [Abstract] Objective  To evaluate the inhibitory effects of a novel tertiary alkaloids small molecule fromSolanum cathayanum , SCE-1，on interleukin (IL-6)，IL-1β and tumor necrosis factor (TNF)-αin vitro . Methods  The inflammation modelin vitro was established by lipopolysaccharide (LPS)-stimulated phrobol 12-myristate 13-acetate (PMA)-differentiated THP-1 macrophage. The cytotoxicity of SCE-1 was determined by the mitochondrial-respiration-dependent3-(4,5-dimethylthiazol -2-yl) -2,5-diphenyltetrazolium (MTT) reduction method. The antagonism activity on proinflammatory cytokine such as IL-6, IL-1β and TNF-α in cell culture medium were determined by enzyme-linked immunosorbent assay (ELISA) method. The inhibitory effect on proinflammatory cytokine of compounds SCE-1 was evaluated. Results  Compared with the control group，(1) the SCE-1(12.5~800 mg/L) showed a significant inhibitory effect in dose- and time-dependent manner on the production of IL-6 (P < 0.01) with an IC50 of 11.82 mg/L; (2) the SCE-1 (200~800 mg/L) decreased the production of TNF-α (P < 0.05 or P < 0.01), and the inhibition rate was 53.13% at a concentration of 800 mg/L and for 2-h treatment; (3) the SCE-1 (400~800 mg/L) significantly increased the production of IL-1β (P < 0.01). Conclusion  The discovery of this com pound provided a potential lead compound for the development of small molecule IL-6 inhibitor with high efficiency and low toxicity. Related Articles | Metrics

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The risk factors related to lower extremity amputations in the patients suffered from severe infective diabetic foot ulcers 2013, 41 (2):  110-113 .  Abstract ( 478 )   PDF (2794KB) ( 2804 )   [Abstract] Objective  To analyze risk factors of lower extremity amputations in patients with severe infective diabetic foot ulcers. Methods  The total of 1 076 patients with diabetic foot ulcers was included in this study. All patients with severe diabetic foot infections were selected (PEDIS grade 3-4) by PEDIS infection classification，and divided into three groups including non-amputation group (group A，n=48),healing after minor amputations (group B，n =162) and administered amputations above ankle-level (group C, n=70). The biochemical indicators and SAD score were measured and compared in groups of patients. The multiple Logistic regression analysis was used to observe the factors affect small amputation (toe) and amputation. Results  The multivariate analysis showed that platelet count, serum uric acid and the degree of peripheral vascular diseases (SAD score) were independent predictors of minor amputations in the patients with severe infections. Besides the above factors，the plasma fibrinogen and the depth of ulcer (scored by SAD) were the factors related to the risk of major amputation. Conclusion  It was found that the platelet count, serum uric acid, plasma fibrinogen, the Charlson score, the degree of peripheral vascular diseases and the depth of ulcer can predict the amputation-risk in diabetic foot patients with severe infections. Related Articles | Metrics

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Correlation between Serum γ-GGT Level and Cardiovascular Risk Factors in Different Genders with Type 2 Diabetes Mellitus 2013, 41 (2):  114-118 .  Abstract ( 619 )   PDF (3412KB) ( 2468 )   [Abstract] Objective  To explore the correlation between serum gamma glutamyltransferase (γ-GGT) and cardio vascular risk factors in different genders with type 2 diabetes mellitus. Methods  A total of 286 patients with type 2 diabetes were divided into male group and female group, and then each group was sub-divided into four groups according to the quartile of serum γ-GGT level. The values of body mass index (BMI), waist, systolic pressure (SBP), diastolic pressure (DBP), triglyceride (TG), cholesterol (TC), high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL), fasting plasma glucose (FPG), glycated hemoglobin (HbA1c), high sensitive c-reactive protein (hsCRP) and uric acid (UA) were compared between groups. Results  There were significant differences in BMI, waist, DBP, TG, TC, UA，γ-GGT，age, HDL-C，HbA1c，hsCRP and plasma fibrinogen (FIB) between different genders with type 2 diabetes mellitus. There were significant differences in age，duration of disease，BMI, waist, SBP, DBP, TG, TC, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in male subjects with different γ-GGT levels. There were significant differences in waist, TC, LDL-C, hsCRP, ALT,AST and alkaline phosphatase (ALP) in female. The level of γ-GGT was negatively correlated with age and duration of disease in male patients. But it was positively correlated with BMI, waist, DBP, TG, TC, hsCRP and UA in male patients. In female patients, the level of γ-GGT was positively correlated with waist, TC, LDL-C, HbA1c, hsCRP and UA. Values of BMI and TC were the independent risk factors of γ-GGT in male patients. Values of waist and hsCRP were the independent risk factors of γ-GGT in female patients. Conclusion  The serum γ-GGT is closely related with several cardiovascular risk factors in patients with type 2 diabetes mellitus, which deserves attention and research in predicting the cardiovascular complication of diabetes. Related Articles | Metrics

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Relationship between plasma anti-Müllerian hormone (AMH) level and body weight, metabolic,and hormonal status in women with polycystic ovary syndrome XU Yan ying1,YIN Li rong2,CHEN Feng zhen1,WANG LI 3, 3 2013, 41 (2):  119-121 .  Abstract ( 622 )   PDF (331KB) ( 2938 )   [Abstract] Objective  To analyze interrelation between anti-Müllerian hormone (AMH) levels and body weight，hormone status and metabolic rate in women with polycystic ovary syndrome (PCOS). Methods  Sixty women (30 normal weight, PCOS 1 group and 30 overweight, PCOS 2 group) diagnosed as PCOS were enrolled and 30 healthy women (15 normal weight and 15 overweight non-PCOS) were as control group．The body weight and height were measured and body mass index(BMI) was calculated. The serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol, testosterone, adrenal medullary hormone (AMH), androgens, fasting glucose and fasting insulin were detected, and the homeostasis model of assessment-insulin resistance (HOMA-IR) were caculated and compared between four groups. Results  The levels of AMH, LH, LH/FSH, testosterone, fasting insulin and HOMA-IR were higher in PCOS group than those of control group. There was no significant difference in AMH level between PCOS 1 and PCOS 2 groups. The levels of LH and LH/FSH were lower in PCOS 1 group than those of PCOS 2 group. The levels of fasting insulin and HOMA-IR were higher in PCOS 1 group than those of PCOS 2 group. There were no correlations in AMH and BMI levels in PCOS group. There were positive correlations in AMH and testosterone, fasting glucose and fasting insulin and HOMA-IR in PCOS 1 group，but a negative correlation between estradiol and FSH. A significant positive correlation was found between LH, LH/FSH, testosterone and AMH in PCOS 2 group (P < 0.05). Conclusion  There is a significant increase in plasma AMH in patients with PCOS. The plasma AMH level is not associated with BMI, which may have correlation with hormone imbalance and metabolic disorders. Related Articles | Metrics

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Effects of Local Mild Hypothermia Therapy On Serum Content of Platelet Activating Factor in Patients with Acute Large Area Cerebral Infarction HOU Dan ,YU Dan 2013, 41 (2):  122-125 .  Abstract ( 575 )   PDF (2881KB) ( 2352 )   [Abstract] Objective  To observe the clinical effects and changes of serum activity of platelet activating factor (PAF) of mild hypothermia therapeutic (MHT) in patients with massive cerebral infarction, and the mechanisms thereof. Methods  Fifty patients with acute massive cerebral infarction diagnosed within 24 hours were randomly divided into hypothermia therapy group and conventional treatment group. The hypothermia therapy group accepted conventional treatment and hypothermia therapy for 48 hours. The neural function deficiency scores and the serum activity of PAF were compared before treatment and 48 hours after treatment. The U.S. National Institutes of Health Stroke Scale (NIHSS) was used to measure the neural function deficiency. Enzyme linked immunosorbent assay was used to detect the serum activity of PAF. Results  After 48- hour treatment，there were significant differences in the PAF activity and NIHSS scores between hypothermia group and conventional treatment group (P﹤ 0.05). Conclusion  The local mild therapeutic hypothermia can obviously improve the neurological function in patients with acute massive cerebral infarction, which may be involved in the decreased activity of serum PAF. Related Articles | Metrics

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Interaction between serum uric acid and other risk factors in relation to hypertension WANG Yuan yuan 2013, 41 (2):  126-129 .  Abstract ( 508 )   PDF (2896KB) ( 2452 )   [Abstract] Objective  To investigate the relationship between serum uric acid and hypertension, and to evaluate the effects of interaction between serum uric acid and other risk factors on hypertension. Methods  The total of 3 318 permanent residents in Tianjin Dagang oilfield were included in this study. All subjects were divided into four groups according to the serum uric acid quartiles. The related factors such as smoking, drinking and family history were investigated. Levels of blood pressure, body mass index (BMI), fasting plasma glucose (FPG), lipid and uric acid were detected and analyzed. Results  The levels of age, blood pressure, BMI, FPG, serum total cholesterol, triacylglycerol and low density lipoprotein cholesterol (LDL-C) were increased with the increase in the level of serum uric acid, but the level of high density lipoprotein cholesterol (HDL-C) was decreased with the increase in the level of uric acid. After adjustment for relevant factors, OR values of hypertension increased with the elevated uric acid levels. The effect of interaction between levels of uric acid and LDL-C was significantly higher in subjects with high levels of LDL-C (P＝0.01). Conclusion  The elevated serum uric acid level was associated with hypertension, which was affected by the interaction between serum uric acid and LDL-C. Related Articles | Metrics

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The study on the inhibition of celecoxib for the transformation of GES-1 cells induced by H.pylori of in vitro QIN Yan 1,ZHANG Zhi guang2,LI Man 2 2013, 41 (2):  130-132 .  Abstract ( 647 )   PDF (2730KB) ( 2369 )   [Abstract] Objective  To investigate the effect of selective cyclooxygenase-2 (COX-2) inhibitor (celecoxib) on the inhibited proliferation and induced apoptosis in gastric epithelial cell transformation induced by Helicobacter pylori (H.pylori) . Methods  In vitro, the immortalized‘normal’gastric epithelial cell line GES-1 was co-cultured for two months with H. pylori Sydney strain (SS1) to establish the GES-1 transformation cell model. The cell transformation was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and colony formation assay. The proliferation and apoptosis of transformed cells treated by celecoxib were detected by MTT assay and flow cytometry. Results  The proliferative activity of GES-1 cells co-cultured with H. pylori (SS1) for two months was significantly higher compared with that of control GES-1 cells. The proliferative activity of GES-1 transformed cells was inhibited with the increased concentration of celecoxib (50，75 and 100 μmol/L), and the apoptotic rate of the GES-1 transformed cells was gradually increased. Conclusion  The transformation of gastric epithelial cells can be induced by H. pylori for a long term. Celecoxib inhibits the proliferation and induce apoptosis of GES-1 transformed cells. Related Articles | Metrics

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The relationship of the serum bilirubin levels and the clinical adverse eventsin patients with acute myocardial infarction LIU Juan 1,QI Xin 2 2013, 41 (2):  130-132 .  Abstract ( 310 )   PDF (1983KB) ( 2053 )   【Abstract】Objective  To evaluate the relationship of the serum bilirubin levels and clinical adverse events in patients with acute myocardial infarction (AMI). Methods  Clinical data including age, body mass index, blood pressure, blood lipids and left ventricular ejection fraction (LVEF) of 109 patients with AMI and 100 control subjects were collected and compared. AMI patients were divided into adverse event group (n=48) and non- adverse event group (n=61). The serum total bilirubin (TBIL), direct bilirubin (DBIL) and indirect bilirubin (IBIL) were detected and compared by collecting 5 mL fasting venous blood. Results  Compared with the control group, values of body mass index, age, gender，blood pressure，blood lipids， fasting blood sugar and other clinical data showed no significant difference in AMI group (P > 0.05). The serum levels of TBIL and DBIL were significantly lower in AMI group than those of control group (P < 0.01). The total serum bilirubin and indirect bilirubin levels were significantly lower in clinical adverse event group compared with those of non-event group (P < 0.05). Conclusion  Changes in serum bilirubin levels have a certain relationship with the clinical adverse events in patients with acute myocardial infarction. Related Articles | Metrics

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The quantity evaluation of left atrial function in coronary heart disease by echocardiography GUAN Xin 2013, 41 (2):  136-138 .  Abstract ( 551 )   PDF (1992KB) ( 2489 )   [Abstract] Objective  To discuss the changes of left atrial function by evaluating the parameters using colour Doppler echocardiography in patients with coronary heart disease. Methods  One hundred and twenty-five patients with chest pain and chest tightness were included in this study, in which there were 90 patients with coronary heart disease (CHD), 35 patients with non-coronary heart disease (NCHD). For patients with CHD, there were 30 patients with single-vessel disease, 30 with double-vessel disease and 30 with triple-vessel disease. Values of left atrial diameter (LAD), area (LAA), volume (LAV), left atrial ejection fraction (LAEF), the velocity of left atrial wall (VA-LA), the velocity of interatrial septum (VA-IAS) were measured by colour Doppler echocardiography in all patients. Results  There were significant increases in LAD, LAA and LAV in patients with CHD. The values of LAEF, VA-LA and VA-IAS were significantly lower in patients with CHD than those in patients with NCHD (P < 0.01). The values of LAA-EF, LAV-EF, VA-LA and LVEF were significantly lower in single-vessel disease group than those in NCHD group. Values of LAD, LAA and LAV were significantly greater, but values of LAA-EF, LAV-EF, VA-LA, VA-IAS and LVEF were significantly lower, in double-vessel and triple-vessel disease group than those of single-vessel disease group and NCHD group (P < 0.001). Conclusion  Coronary heart disease can impair left atrial function, which was aggravated with the severity of coronary lesions. The value of VA-LA was the sensitive parameter for the change assessment of left atrial function in coronary heart disease. Related Articles | Metrics

实验研究

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Effects of Thyroid Hormone on the Expression of Homeobox Gene Nkx6.2 in the Cerebrum Tissue of Offspring Rat of Hypothyroidism Pregnant Mother 2013, 41 (2):  139-141 .  Abstract ( 492 )   PDF (2142KB) ( 2308 )   [Abstract] Objective  To explore the effects of thyroid hormone and complement of levothyroxine (L-thyroxine, L-T4) to pregnant mother on the expression of homeobox gene Nkx6.2 mRNA in rat offspring. Methods  One hundred and twenty Wistar rats were randomly divided into normal control group, hypothyroidism group, hypothyroidism in early pregnancy group (1-17 d) supplied with high dosage, medium dosage and low dosage L-T4 and hypothyroidism in late pregnancy group (18-20 d) supplied with high dosage, medium dosage and low dosage L-T4 (15 rats for each groups). The brain tissues of offspring were taken at gestation day17, postnatal day 0 and postnatal day 20, and the content of Nkx6.2 mRNA was detected by real-time fluorescence quantitative PCR techniques. Results  There was significant difference in Nkx-6.2 expression level between hypothyroidism group and normal control group (P < 0.01). There were significant differences in Nkx-6.2 expression levels between groups with supplement of high dosage, medium dosage and low dosage L-T4 and control group (P < 0.01). Conclusion  The expression of Nkx6.2 mRNA is highly related to the supplemental thyroxine dosage and time impact in hypothyrodism preganant rats. Related Articles | Metrics

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Increased Phosphorylation of CaMKⅡ in Penumbra Cerebral Tissues of HPC-MCAO Mice LI Hai-Tao 2013, 41 (2):  142-144 .  Abstract ( 488 )   PDF (2243KB) ( 2340 )   [Abstract] Objective  To investigate the effect of hypoxic preconditioning (HPC) on middle cerebral artery occlusion (MCAO)-induced brain injury in mice，and changes of calcium/calmodulin-dependent protein kinaseⅡ(CaMKⅡ) phosphorylation thereof. Methods  Thirty-six healthy male BALB/c mice were randomly divided into 4 groups: the normoxic sham surgery (H0-sham) group, HPC-sham group, H0-ischemia group and HPC-ischemia group. Changes of CaMKⅡ phosphorylation in the brain of mice were detected by Western blot combined with Gel Doc imagine systems. Levels of CaMK Ⅱ phosphorylation in brain cortex of mice were detected by immunohistochemistry. Results  Both phosphorylation and protein expression levels in the ischemic core and penumbra were significantly decreased in H0-ischemia group compared with those in H0-sham group (P < 0.05). But the phosphorylation levels of CaMKⅡ and the positive cell number of p - CaMK Ⅱin the penumbra were significantly higher in HPC-ischemia group than those of H0-ischemia group (P < 0.05). Conclusion  The de? creased CaMKⅡ phosphorylation level in ischemia penumbra might involve in the attenuation of middle cerebral artery occlusion (MCAO)-induced brain injury in mice. Related Articles | Metrics

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The therapeutic effect on ischemia heart disease by hypoxic preconditioning of endothelial progenitor cells transplantation JIAN Kai tao1,WANG Lian qun1,WANG Qiang 1,Bao-rong Zheng2,LIU Jianshi 1 2013, 41 (2):  145-147 .  Abstract ( 550 )   PDF (2331KB) ( 2379 )   [Abstract] Objective  To investigate the therapeutic effectiveness of transplantating hypoxic preconditioning endothelial progenitor cells (HEPCs) on acute myocardial infarction. Methods  Bone marrow endothelial progenitor cells (EPCs) were isolated from 8-week syngeneic adult male Wistar rats. After normoxic culture for 4 days, EPCs were cultured in 1% O2+ 5% CO2+94% N2 for 3 days. Then, 26 8-week syngeneic adult male Wistar rats were randomized into 3 groups: control group (n=8)，EPCs group (n=9) and HEPCs group (n=9). The acute myocardial infarction animal model was set up. Rats received 5-points peri-infarct intramyocardial injections of PBS 200 μL 2×106 EPCs and 2×106 HEPCs. After 4 weeks, the pressure-volume (P-V) curve analysis was used to evaluate the haemodynamics parameters and cardiac function. Results  After 4 weeks of transplantation, P-V loop analysis showed that there were significant differences in end diastolic volume, end systolic volume, stroke volume,ejection fraction( EF) and cardiac output between HEPCs and control groups. There were significant differences in end systolic volume, stroke volume and cardiac output between HEPCs and EPCs groups. There were no significant differences in stoke volume and cardiac output between EPCs and control groups. Conclusion Transplantation of HEPCs limited left ventricular remodeling and improved cardiac function entirely after acute myocardial infarction. Related Articles | Metrics

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Tumorigenicity of Human Mesenchymal Stem Cells from Adipose Tissue 2013, 41 (2):  152-153 .  Abstract ( 548 )   PDF (2444KB) ( 2923 )   [Abstract] Objective  To study the tumorigenicity of human mesenchymal stem cells from adipose tissue (hMSC-AT), and to provide safety data for its clinical application. Methods  Ninety-six nude mice were divided into blank control group， positive control group and adipose tissue-derived mesenchymal stem cell group (n=32 for each group). The hMSC-AT of the 5th passage was subcutaneously injected into nude mice. The mice were sacrificed 3 days, 1 month, 3 months and 6 months after injection respectively. The anatomical check was carried out to observe the tumor formation, and the ability to form colonies in hMSC-AT was observed by soft agar cloning method. Results  No abnormal cells were found by gross necropsy and histological examination in animals of hMSC-AT group and blank control group. In vitro test, hMSC-AT did not show the capacity for clonal growth in the medium. Conclusion  The hMSC-AT at low passage appears to be safe and is not tumorigenic. Related Articles | Metrics

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Effect of Decitabine Combined with ATRA on Proliferation and Differentiation of HL-60 Cells LU Hai rong 2013, 41 (2):  154-157 .  Abstract ( 590 )   PDF (2723KB) ( 2671 )   [Abstract] Objective  To investigate the effect of demethylation agent decitabine (DCA) and all-trans retinoic acid (ATRA) on proliferation and differentiation of acute promyelocytic leukemia (APL) cell line HL-60. Methods  HL-60 cells were co-cultured with different concentrations of DCA and/or ATRA. The proliferation activity was detected by MTT assay in HL-60 cells. The differentiation ability of HL-60 cells was observed by nitro-blue tetrazolium (NBT) reduction test and flow cytometry. Results  The proliferation of HL-60 cells was inhibited by DCA and ATRA in a dose and time dependent manner (P < 0.01). The concentration of 3.2 mmol·L-1 DCA combined with 1×10-6 mol·L-1 ATRA showed a more obvious inhibition on proliferation of HL-60 cells compared with that of DAC or ATRA alone (P < 0.05). Both DCA and ATRA could promote differentiation of HL-60 cells. The strongest ability in promoting differentiation was found in 3.2 mmol·L-1 DCA group and 1× 10-6 mol·L-1 ATRA group.The combination of DCA and ATRA had synergistic effect. Conclusion  Both DCA and ATRA can inhibit the proliferation and promote the differentiation of HL-60 cells, and the combination of DCA and ATRA had synergis? tic effect. Related Articles | Metrics

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The influence of alcohol on osteoblast apoptosis in vitro 2013, 41 (2):  158-161 .  Abstract ( 543 )   PDF (3341KB) ( 2390 )   [Abstract] Objective  To study the influence of alcohol on osteoblast apoptosis in vitro. Methods  The mouse calvarial osteoblasts were cultured in vitro to three generations and divided into two groups: the control group and intervention group. Cells of control group were cultured in 1640 medium containing 10% FBS, and cells of intervention group were added 100 mmol/L alcohol after 1 day normal culture. After 1，3，5 and 7d culture, cells were harvested to measure MTT value. After 3，7，10 and 14 d culture, cells were harvested to measure alkaline phosphatase (ALP) and osteocalcin (BGP) activity. After 1 d culture, the osteoblast apoptosis was assessed by flow cytometry. Results  There was no significant difference in the value of MTT between two groups (P ＞ 0.05). The cellular proliferation rate was significantly lower in intervention group after 3, 5 and 7 d culture (P ＜ 0.01). The values of ALP and BGP significantly increased in control group and decreased in intervention group after 3, 7, 10 and 14 d culture (P < 0.05 orP < 0.01). The values of ALP and BGP were significantly lower in interven? tion group than those in control group after 3, 7, 10 and 14 d culture (P < 0.01). The apoptotic rate was significantly higher in intervention group than that in control group after 1 d culture (P < 0.01). Conclusion  Alcohol had a marked effect on osteo? blast apoptosis by weakening the proliferation and differentiation. Related Articles | Metrics

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Different Position of Clear Corneal Incision Phacoemulsification Postoperative Astigmatism Analysis WU nq 2013, 41 (2):  162-163 .  Abstract ( 535 )   PDF (2042KB) ( 2368 )   Different Position of Clear Corneal Incision Phacoemulsification Postoperative Astigmatism Analysis Wu Na Zhang Rui. The First Center Hospital,Tianjin,300192,China Abstract Objective：Comparison of different position of clear corneal incision phacoemulsification and astigmatic changes after operation. Methods：The age-related cataract patients in 70 cases (70 eyes) were divided into two groups, group A patients using temporal above ( right) or nasal incision above (left), B group according to the curvature of the Cornea Corneal radial axial do under the guidance of the largest clear corneal incision. All the patients underwent phacoemulsification combined with intraocular lens implantation after operation respectively,1 weeks,1 months, and 3 months follow-up of patients,patients were examined for vision and corneal topography . Results: After 3months in B group than these in A group of uncorrected visual acuity,B group of patients after 1 weeks of corneal astigmatism changes less than group A. Conclusion: According to the cornea diopter axial orientation maximum radial make clear corneal incision phacoemulsification on postoperative corneal astigmatism, the impact of small, can achieve early refractive stability. Related Articles | Metrics

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Low-temperature plasma treatment for epiglottis cyst under microscope (120 cases report) 2013, 41 (2):  163-165 .  Abstract ( 780 )   PDF (1391KB) ( 2710 )   Abstract Objective  To explore the application under the microscope low-temperature plasma treatment epiglottis cyst of therapeutic effect, safety and application experience. Methods： Retrospective analysis I branch since June, 2008 to June, 2011 application low-temperature plasma treatment epiglottis cyst in 120 cases, the general endotracheal intubation, under the laryngoscope support, monitoring system in operation microscopes under low temperature plasma treatment epiglottis cyst application, observe the operation safety and efficacy. Results：All the group of 120 patients, average operation time （16.5±1.6）min, the first day of the mean pain score （3.6±0.5）, average peri-operative bleeding （2.7±0.3）ml, only one case in the fourth day after mild ooze blood, average postoperative wound white film subsidise time （4.9±0.9）d, patients were followed up 6 ~ 12 months, no one was recurrence. Conclusion：Under the microscope low-temperature plasma treatment epiglottis cyst operation time is short, pain, small, perioperative, less bleeding, and the results were satisfactory, high safety, is a kind of very good operation. Related Articles | Metrics

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The incidence of diabetes in 299 cases of patients with fatty liver disease in the course of 5 years follow-up 2013, 41 (2):  166-167 .  Abstract ( 556 )   PDF (1932KB) ( 2414 )   Object: Explore the incidence of diabetes in patients fatty liver disease at followed-up 5 years. Method: he crowd was used as the research objects who their first medical examination were performed during 2005.1-2006.12.in our hospital, the examining again before 2011.12.and having complete data, eliminating individuals with HBSAg (+), blood glucose abnormalities and massive alcohol intake. Some index were measured in all participants including height, weight, blood pressure, BMI, fasting blood sugar and blood fat, liver function and insulin levels, electrocardiogram and liver ultrasonography inspection. The objects were divide into the fatty liver disease group and the non fatty liver disease group according to liver ultrasonography inspection results at baseline levels, the insulin concentrations were divided into three levels:< 10ulu/ml, 10-15ulu/ml and >15ulu/ml. Comparison the change of the above indexes and incidence of diabetes between the two groups at follow-up 5 years. Result: (1) The incidence of fatty liver disease was 27.16% by color dopplar ultrasound diagnosis in our data at Baseline level. (2) There were statistically significant differences on age, gender, BMI, blood pressure, blood fat and blood sugar, ALT and AST between the two groups; the fasting insulin concentrations of the majority individuals were >15ulu/ml in fatty liver disease group , it were <10ulu/ml in non fatty liver disease group at Baseline level. (3) The total incidence of diabetes was 10.53% at 5 years of follow-up, having higher incidence of diabetes compared with general population, it was higher in the fatty liver disease group (16.7% vs 8.2%) with statistically significant. (4)Further analysis shows that there were the highest incidence of diabetes in fatty liver disease group with insulin >15ulu/ml level and it was the lower in non fatty liver disease group with insulin levels<10ulu/ml. At the same insulin levels, the incidence of diabetes was higher in fatty liver disease group. with insulin levels rise, the diabetes rate increases, the related analysis shows the difference having statistically significant. Conclusion: The fatty liver disease may involve in the course of adjusting the risk of developing diabetes in individual with insulin resistance. Related Articles | Metrics

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Renal Sympathetic Denervation Follow-up at 6 Months 2013, 41 (2):  168-170 .  Abstract ( 575 )   PDF (2114KB) ( 2278 )   Abstract Objective: To evaluate the safety and efficacy of Renal sympathetic denervation in resistant hypertension through analyzing patient’s blood pressure and renal function at six months. Methods: Follow up 11 cases at baseline, 3 days after the surgery, and six months. To analysis their clinical characteristics including risk factors, BP level, renal function and compared with the baseline. Result: Compared to baseline, the systolic and Diastolic blood pressure were significently lower at intraoperative than baseline, and the BP remain low level in 6 months after surgery, P＜0.001. Conclusions: Effects of renal sympathetic denervation on blood pressure is notable, and safety which proved by follow-up. The findings provide new insights into mechanisms of disease, and show a way for the growing worldwide population of resistant hypertension. Related Articles | Metrics

药物临床观察

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A retrospective analysis of Paclitaxel plus Oxaliplatin and Fluorouracil in the treatment of advanced gastric cancer of 94 cases 2013, 41 (2):  171-171 .  Abstract ( 1004 )   PDF (1203KB) ( 2340 )   [Abstract] Objective: In recent years there have been research reports indicate that paclitaxel oxaliplatin can improve the efficiency of advanced gastric cancer and survival, adverse reactions can be tolerated. And oxaliplatin combination with fluorouracil have synergistic effects. This group of paclitaxel, oxaliplatin, fluorouracil is in the treatment of advanced gastric cancer, aims to explore the Clinical efficacy of advanced gastric cancer and adverse reactions. Methods: Undergraduate from January 2005 to January 2010, 94 advanced gastric cancer patients were enrolled. Chemotherapy dose and method of setting: paclitaxel (PTX )135 mg/m2 d1, fluorouracil (5-Fu) 500 mg/m2, intravenous continuous infusion chemotherapy pump d2-6, oxaliplatin 100mg/m2, intravenously 3h, d2. strict accordance with the instructions before using paclitaxel pretreatment. 21 days for one cycle, two cycles were evaluated.. Results: Whole group of 94 patients total effective rate was 52.1% (49/94), complete response rate was 1.0% (1 / 94), partial remission rate was 51.1% (48/94), stable was 23.4% (22/94) , progress was 24.5% (23/94). The median time to progression was 7.2 months, median survival time of 11.3 months. No treatment-related deaths, mainly in peripheral blood cells to decrease toxicity, gastrointestinal reactions, alopecia and peripheral neurotoxicity..Conclusions: Paclitaxel, oxaliplatin, fluorouracil in the treatment of advanced gastric cancer is effective, patients can delay disease progression, and toxicity can be tolerated, has clinical value. Related Articles | Metrics

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Efficacy of Isotretinoin in the treatment of severe acne 2013, 41 (2):  172-174 .  Abstract ( 1284 )   PDF (1344KB) ( 2904 )   Tang zhiling,Dermatology department of Tianjin Jixian people's Hospital （301900） [Abstract] Objective To observe the efficacy of Isotretinoin in the treatment of severe acne . Methods The persons being tested were randomly divided into two groups , body weight ≥ 50kg in the test group, every other day took orally 10mg Isotretinoin , 3 times a day ; body weight <50 kg , every other day took orally 10mg Isotretinoin 2 times a day . Control group : 50mg minocycline hydrochloride twice daily . Treatment time of the both two groups of patients lasted for 8 weeks .At the 2th,4th and 6th week in the treatment and the 1th, 2th, 4th, 6th and the 8th week after the treatment, we compared efficiency scores respectively between the two groups of patients . Results At the 2th,4th,6th and the 8th week in the treatment , we compared the test group and the control group , the effective rate was significantly different . At the 4th and 6th week after the treatment,we compared the test group with the control group,the effective rate was still significantly different.Conclusions Isotretinoin in the treatment of severe acne was more effective than minocycline . Related Articles | Metrics

临床论丛

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Generalized Arterial Calcification in Infancy:A Case Report and Review of the Literature 2013, 41 (2):  175-176 .  Abstract ( 1243 )   Abstract: Objective To report a case of rare generalized artery calcification in infant (GACI) with the clinical characteristics and diagnostic experience, and to enhance the understanding of the disease accordingly. Methods A case of a neonate, boy, with GACI was analyzed in clinical manifestation, auxiliary examination and process of treatment. We have also reviewed the relevant literature. Results The affected infant hospitalized for twice, performing for the dyspnea frequently, heart failure, and shock. The clinical symptoms improved after treatment, accompanying with the syndrome of hypertension, and then for the further CT examination showed generalized calcifications of the cardiac and thoracoabdominal aorta wall, finally the patient was diagnosed GACI. Conclusion GACI is a rare genetic disorder consisting of diffuse arterial calcification and intimal proliferation. The disease typically results in progressive arterial stenosis and frequently leads to death from myocardial ischemia within 6 months of life. Affected infants are usually diagnosed before birth or in the neonatal period with symptoms of congestive heart failure. GACI can be diagnosis by the pathologic biopsy or imaging. Therapy with bisphosphonate has been used to treat the condition, but with inconsistent results. The disease is associated with mutations in ENPP1 gene in the majority of the cases. Related Articles | Metrics