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15 September 2012, Volume 40 Issue 9 Previous Issue    Next Issue
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短篇论著
论著
实验研究
短篇论著
临床论丛
综述与讲座


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短篇论著

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The study of peripheral blood lymphocyte subsets and serum IL-6, IL-10 in children with hand-foot-mouth diseases 2012, 40 (9):  0-0 .  Abstract ( 1036 )   【Abstract】Objective To explore the pathogenesis of hand-foot-mouth disease(HFMD)，the changes of lymphocyte T subsets and the level of serum interleukin-6 (IL-6) and interleukin-10 (IL-10) were determined. Methods The changes of lymphocyte T subsets(CD3+、CD4+、CD8+、NK、CD4+/CD8+) and the level of serum IL-6，IL-10 were assayed in 82 patients of HFMD and 30 normal controls respectively by flow cytometry and by enzyme-linked immuno sorbent assay(ELISA). Results Simple cases of HFMD compared with control group: The percentages of lymphocyte CD3+,CD4+ were significantly reduced（p<0.01）, and no significant difference was observed in the percentages of CD8+, natural killer(NK)cells and the ratio of CD4+/CD8+ between simple patients and controls(P>0.05). The ratio of lymphocyte B showed significant increases（p<0.01）, while serum IL-6，IL-10 were detected no significant difference among simple cases and controls（p>0.05）; Severe cases of HFMD compared with control group: The percentages of lymphocyte CD3+,CD4+ and the ratio of CD4+/CD8+ were significantly reduced（p<0.01）, and no significant difference was observed in the percentages of CD8+ and NK cells between severe cases and controls（p>0.05）. The ratio of lymphocyte B and serum IL-6, IL-10 showed significant increases（p<0.01）. The results of severe cases compared with simple cases were similar to compared with controls. Conclution Lymphocyte T in children with HFMD appearred relatively inhibition in varying degrees, lymphocyte B proliferation in response ,and cytokines excessively secretion. The above indicated that the immunity of children with HFMD, especially severe cases, existed dysfunction obviously. Related Articles | Metrics

论著

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The Evaluation of Postoperative Analgesia with Dezocine and Fentanyl in Patients with Obstructive Sleep Apnea Syndrome 2012, 40 (9):  0-0 .  Abstract ( 751 )   PDF (174KB) ( 2069 )   Abstract Objective：To evaluate the analgesic efficiency and safety of patient-controlled analgesia (PCIA) effects with dezocine and fentanyl following uvulopalatopharyngiplasty (UPPP) surgery. Methods: Forty ASA I～Ⅱpatients undergoing UPPP surgery were randomly divided into two groups equally with 20 cases each: group F (fentanyl 16μg／kg) and group D (dezocine 0.8mg/kg). The drugs including 5mg of droperidol were diluted with normal saline to 100 ml and were given via PCIA after surgery. The PCIA rate was 2 ml／h, bolus 1.0 mL, lockout time 15 min. Postoperative analgesia efficiency was assessed by Visual Analogue Scale (VAS) and Ramsay score at 1, 3, 6, 12, 24h. The circulatory, ventilatory function , PCIA pressing times and side effects were also recorded. Results：The patients in both groups showed good pain relief. In group F, VAS scores were lower while Ramsay scores were higher than those of group D at 1h (P < 0.05). There were no significant differences in VAS and Ramsay scores in the two groups at the other time (P > 0.05). In the two groups, there were no significant differences in PCIA pressing times (P > 0.05). In group F, MAP and HR were lower than those of group D at 1-3 h (P < 0.01或0.05). In group F, SPO2 were lower than that of group D at 1h (P < 0.01). In group F, the nausea and vomiting and the respiratory depression were higher than those of group D (P < 0.05). There were no significant differences in other side effects in the two groups (P > 0.05). Conclusion: Comparing with fenatanyl, postoperative patient-controlled intravenous analgesia with dezocine may be the preferred analgesic following UPPP surgery. Related Articles | Metrics

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Role of XPD in Hepatoma Cells regulating Ets-1 and Cdk6 2012, 40 (9):  865-869 .  Abstract ( 885 )   PDF (307KB) ( 2395 )   Objective：To transfect wild-type XPD gene into human hepatoma cell line SMMC-7721 and to observe the expression of Ets-1 and Cdk6 gene and the influence in the growth and proliferation of SMMC-7721 cells. Methods：The pEGFP-N2-XPD was transfected into SMMC-7721 cells by Lipofectamine 2000TM. There were four groups in my study including SMMC-7721-pEGFP-N2-XPD group(XPD group)，SMMC-7721-pEGFP-N2 group(N2 group)，Lipofectamine group(Lip group) and blank control group. The expressions levels of XPD，Ets-1 and Cdk6 were detected by RT-PCR and Western blot. Flow cytometry(FCM) was used to analyze the cell cycle of SMMC-7721 cells. The cell proliferation was measured by MTT assay. Results: Compared with blank control group，N2 group and Lip group，the expression of XPD mRNA and protein was enhanced significantly in XPD group(P<0.01)，the expression of Ets-1 and Cdk6 mRNA and protein were decreased obviously(P<0.01). FCM showed that XPD prevented the hepatoma cells from G1 stage to S stage. The proliferation ability of SMMC-7721 cells was observably reduced after transfected by wild-type XPD gene(P<0.01). Conclusion: XPD gene may regulate the hepatoma cell proliferation by inhibiting the Ets-1 and Cdk6 gene. Related Articles | Metrics

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Relationship of Nup88 and PINCH in colorectal carcinoma 2012, 40 (9):  870-872 .  Abstract ( 853 )   PDF (174KB) ( 2386 )   Abstract: Objective To study the nucleoporin 88 (Nup88) and PINCH mRNA level in colorectal carcinoma and cell lines, and further to investigate the potential correlation between them. Methods Nup88 and PINCH mRNA were detected by RT-PCR in 47 colorectal carcinomas, 44 adjacent non-cancerous mucosa, 45 proximal surgical margin, 44 distal surgical margin and 6 colon cell lines. The correlation study of Nup88 and PINCH was carried out by Spearman analysis. Results Nup88 and PINCH mRNA was positive correlated in colorectal carcinoma, adjacent non-cancerous mucosa, proximal and distal surgical margin. In addition, there was a positive correlation trend between Nup88 and PINCH in colon cell lines(r=0.6, P=0.24). Conclusion Nup88 and PINCH were positive correlated at mRNA level, and they might play a role in the tumorigenesis and development of colorectal cancer. Related Articles | Metrics

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Expression and Mutation analysis of FAT10 gene in Human Hepatocellular Carcinoma 2012, 40 (9):  873-875 .  Abstract ( 797 )   PDF (255KB) ( 2494 )   0bjective: To discuss the expression and mutation of FAT10 gene in hepatocellular carcinoma．Methods: The FAT10 expression levels were examined by Western blot in 60 specimens of human HCC and its corresponding adjacent non-cancerous tissues．DNA sequencing was used to detect FAT10 gene coding region mutation in 60 HCC patients including cancer and adjacent non-cancerous tissues and 5 cell lines of human HCC．Results：We examined FAT10 expression levels in 40 pairs of human HCC and their corresponding nontumorous pancreas. FAT10 was found to be overexpressed in 85% of the HCC cases (p<0.05). Seven SNPs in FAT10 gene coding region were identified, No mutation was found in all HCC patients. Conclusion: FAT10 gene was highly expressed in Chinese HCC patients．No mutations of FAT10 gene were detected in HCC sample tissues and HCC cell lines．It indicated that FAT10 gene was involved in the process of hepatocarcinogenesis and this process was independent on gene mutation, maybe by other mechanisms．It needs further study Related Articles | Metrics

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Mechanism of isomycin derivant on apoptosis of human breast carcinoma MCF-7 cell line 2012, 40 (9):  876-877 .  Abstract ( 825 )   PDF (200KB) ( 2343 )   Objective: To investigate the mechanism of isomycin derivant on anti-proliferation and inducing apoptosis of human breast carcinoma MCF-7 cell line in vitro. Methods: MCF-7 cells were cultured in vitro; We investigated the expression of Fas、Caspase-3、Caspase-8、NF-kB by immunohistochemitry and studied the possible mechanism of the apoptosis. Results: Immunocytochemistry showed that Isomycin derivant up-regulated the expression of Fas、Caspase-8、Caspase-3，and they were significantly different from control groups; Isomycin derivant also inhibited signal transduction pathway of NF-kB. Conclusions: Isomycin derivant induced apopsis via extrinsic or death receptor-mediated pathway; Inhibition of the signal transduction pathway of NF-kB may be the mechanism of inducing apoptosis. Related Articles | Metrics

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Differential diagnosis of lung masses with integrated 99Tcm-MIBI-SPECT/CT 2012, 40 (9):  878-881 .  Abstract ( 892 )   PDF (228KB) ( 2443 )   Objective: To investigate the differential diagnostic value of integrated 99Tcm-MIBI-SPECT/CT after oxygen inhalation for lung masses. Methods: From December 2008 to September 2011, we had prospectively studied 34 consecutive patients with lung masses. All patients breathed oxygen via nasal catheter before 99Tcm-MIBI injection. Ten minutes and 2 hours after 99Tcm-MIBI injection, early and delayed SPECT/CT fusion imaging of the chest was performed, respectively. The uptake ratios of malignant and benign lung masses were compared by independent-samples T test. In addition, the diagnostic efficiency of uptake ratios of lung masses was analyzed with receiver operating characteristic (ROC) curve. Results: There were 27 patients with malignant lung lesions and 7 patients with benign lung lesions. The sensitivity, specificity, accuracy, positive predictive value (PPV), and negative predictive value (NPV) of qualitative analysis were 100%, 71.43%、94.12%, 93.10% and 100%, respectively. In patients with malignant lung lesions, the early mean T/N ratio value ± standard deviation was 3.29±2.10. Whereas in patients with benign lung lesions, that was 1.55±0.66. In patients with malignant lung lesions, the delayed mean T/N ratio value ± standard deviation was 3.40±2.05. Whereas in patients with benign lung lesions, that was 1.59±0.61. The early and delayed T/N ratio were significantly different between malignant lung lesions and benign lung lesions (t=2.14, t=2.28, respectively. P＜0.05). Semiquantitative analysis showed that for a early T/N value ≥1.87, the value of sensitivity and specificity was 92.60% and 71.40%, respectively (ROC curve), for a delayed T/N value ≥1.78, the value of sensitivity and specificity was 100% and 71.40%, respectively (ROC curve). Conclusion: Our study shows that integrated 99Tcm-MIBI-SPECT/CT takes on highly clinical value in differentiating malignant from benign lung masses, and it is a practical method for medium-to-low income patients with lung masses in small and medium-sized cities. Related Articles | Metrics

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The Changes of Ratio of Serum IL-8/IL-10 after Percutaneous Coronary Intervention and the Related Factors 2012, 40 (9):  882-884 .  Abstract ( 1002 )   PDF (170KB) ( 2453 )   Abstract Objective: To investigate the changes of ratio of serum IL-8/IL-10 after percutaneous coronary intervention (PCI) and the related factors. Methods: A total of 103 patients undergoing coronary angiography (CAG) were recruited, who were divided into SA1 group (patients with stable angina only undergoing CAG, n=31), SA2 group (patients with stable angina undergoing PCI, n=34), and UA group (patients with unstable angina undergoing PCI, n=38). Blood samples were taken in order to evaluate serum concentrations of high-sensitivity C-reactive protein (hs-CRP), interleukin (IL)-8 and IL-10 just before PCI and 24 h after the procedure. Results: (1) In both SA2 and UA group, the ratio of IL-8/IL-10 after PCI was higher than that before PCI (P < 0.05, P < 0.01). (2) After PCI, the ratio of IL-8/IL-10 in the UA group was higher than that in both SA1 and SA2 group (P < 0.001). (3) The ratio of IL-8/IL-10 before PCI and the situation of unstable angina correlated with the ratio of IL-8/IL-10 after PCI. Conclusion: There exists inflammatory cytokine imbalance after PCI, which triggers a systemic inflammatory response. Moreover, this response is more obvious in patients with inflammatory cytokine imbalance before PCI and unstable angina. Related Articles | Metrics

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Correlation between the polymorphism of 3′-UTR of Lectin-like Oxidized Low-density Lipoprotein Receptor-1 gene and coronary artery disease in Tianjin Population 2012, 40 (9):  885-888 .  Abstract ( 874 )   PDF (187KB) ( 2409 )   Abstract Objective: To investigate the polymorphism of 3′-UTR 188 site of lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) gene and to explore the correlation between the gene polymorphism of LOX-1 and coronary artery disease（CAD）in the population of Tianjin. Methods: A total of 170 cases confirmed by coronary arteriograghy（CAG） were included in the CAD group and 140 healthy subjects confirmed by CAG were included in the control group. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to analyze the polymorphism of 3′-UTR 188 site of LOX-1 gene and to compare its relationship with coronary artery disease between the two groups. Results: There were no significant difference of genotype frequency and allele frequency of 3′-UTR 188 site of LOX-1 between the the CAD group and control group (P>0.05). Conclusion:There is no significant correlation between the polymorphism of 3′-UTR 188 site of LOX-1 and coronary artery disease, therefore the polymorphism can not be confirmed as a predictor of coronary artery disease in the population of Tianjin. Related Articles | Metrics

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The Correlation of Serum Electrolytes with Severity of Angiographic Coronary Artery Disease 2012, 40 (9):  889-891 .  Abstract ( 953 )   PDF (205KB) ( 2413 )   Objective: To discuss the relationship between the level of serum electrolytes(K+, Na+, Cl-) and severity of angiographic coronary artery disease. Methods: The study population consisted of 264 patients who underwent coronary angiography for suspected CAD was divided into two groups, the coronary artery disease (CAD) group and the non-CAD group. The CAD group was divided into acute myocardial infarction (AMI) subgroup and non-AMI subgroup. Then to compare the level of serum electrolytes(K+, Na+, Cl-) and other main measurements between two groups and two subgroups. Meanwhile to evaluate the correlation analysis result between all measurements and the number of diseased coronary arteries. Results: The serum potassium level of CAD group was higher than that in non-CAD group (P<0.05), on the contrary, the serum sodium and chlorine concentration of CAD group were both lower than those in another group(P<0.05 or P<0.01). The serum potassium level of AMI subgroup was higher than that in non-AMI subgroup (P<0.01), the serum chlorine concentration of AMI subgroup was lower than that in another subgroup (P<0.05).There was no statistical difference of serum sodium concentration between two subgroups. The correlation analysis indicated that serum potassium level positively correlated with the number of diseased coronary arteries (P<0.05), but sodium and chlorine levels negatively correlated with the number of diseased coronary arteries(P<0.01). Conclusion: Increasing of K+ level and decreasing of Na+ and Cl- levels had correlation with severity of angiographic coronary artery disease. Related Articles | Metrics

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Prognostic Value of the Plasma Levels of type II secretory phospholipase A2 in Patients with Acute Myocardial Infarction Undergoing Primary Percutaneous Coronary Intervention ,Wei WANG 2012, 40 (9):  892-894 .  Abstract ( 1052 )   PDF (170KB) ( 2345 )   Abstract Objective: To evaluate the prognostic value of the plasma levels of type II secretory phospholipase A2 in patients with acute myocardial infarction undergoing primary percutaneous coronary intervention. Methods: A total of 223 patients with AMI who underwent primary PCI were divided into MACE group（95） and no MACE group （128）on the basis of whether happening MACE after 2 years’ follow-up. Compared the baseline characteristics, plasma levels of sPLA2 and angiographic characteristics and procedure related data in patients with PCI of the two groups. Cox's proportional hazard regression analysis was used to assessed the predictive value for MACE during the follow-up period and the Kaplan–Meier method was applied in survival analysis according to the levels of sPLA2. Results: 95 patients happened MACE during the follow-up period. The proportion of male, type 2 diabetes mellitus, number of white blood cells, BUN, Glucose, CK, CK-MB, sPLA2, multiple (≥double-vessel CAD) coronary arteries with stenosis, symptom-to-balloon time and no-reflow after PCI were significantly higher in MACE group than no MACE group while the left ventricular ejection fraction was lower. Multivariate forward stepwise Cox proportional hazard regression analysis showed that sPLA2 >5.48μg/L(RR: 3.23, 95% CI: 1.24-12.36, P<0.001), diabetes mellitus (RR: 2.78, 95% CI: 1.52-7.24, P<0.001), multiple coronary arteries with stenosis (RR: 2.37, 95% CI: 1.66-6.79, P<0.001)and left ventricular ejection fraction <50%(RR: 1.74, 95% CI: 1.07-2.94, P=0.037) were predictors of subsequent MACE. Conclusion: The present study showed that elevation of circulating levels of sPLA2 in patients with acute myocardial infarction undergoing primary percutaneous coronary intervention was associated with the poor prognosis. Related Articles | Metrics

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The Incidence and Risk Factors of Malignant Ventricular Arrhythmia in Patients with Acute Myocardial Infarction 2012, 40 (9):  895-898 .  Abstract ( 940 )   PDF (184KB) ( 2632 )   Objective:To explore the incidence and risk factors of malignant ventricular arrhythmia in patients with acute myocardial infarction. Methods:A retrospective study was performed on 650 patients with acute myocardial infarction from January 2007 to June 2011. According to the Holter outcomes，the patients with acute myocardial infarction were divided into malignant ventricular arrhythmia(MVA) group and non-MAV group.Then incidence rate of MVA were measured and age, gender, history of smoking, hypertension, diabetes, hyperlipidemia, history of old myocardial infarction, creatine kinase, creatine kinase MB, Troponin I, white blood cell count, neutrophil count ,platelet count, hemoglobin, triglycerides, total cholesterol, low density lipoprotein, high density lipoprotein, potassium, low blood potassium, blood urea nitrogen, serum creatinine, blood glucose, Killip class, corrected Tpeak-Tend interval, heart rate variability, opening of infarct-related artery by direct PCI, ultrasound results of convalescence were recorded and determined by univariate correlation analysis and Logistic regression analysis．Results: Among the 650 patients, MAV occurred in 216 patients and the incidence of MAV was 33.2%. Logistic regression analysis showed that creatine kinase, Troponin I, low blood potassium, Killip class, corrected Tpeak-Tend interval, heart rate variability,not opening of infarct-related artery by direct PCI were risk factors of MAV.Conclusion: The incidence of malignant ventricular arrhythmia in patients with acute myocardial infarction was higher and was a major cause of death, Adequate clinical attention should be paid to patients who have the above risk factors of MAV. Related Articles | Metrics

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Comparison of Different Acute Myocardial Infarction Patients Undergoing Early Intervention 2012, 40 (9):  899-901 .  Abstract ( 1010 )   PDF (146KB) ( 2473 )   Objective: To compare the coronary flow, reperfusion of the ischemic myocardium and cardiac function of patients with acute non-ST elevation myocardial infarction (NSTEMI) and ST elevation myocardial infarction (STEMI) who received early invasive intervention strategy. Methods: A total of 168 patients with acute myocardial infarction undergoing early coronary intervention were enrolled, including 55 patients in NSTEMI group and 113 patients in STEMI group. The myocardial blush grade (MBG), pre-intervention thrombolysis in myocardial infarction (TIMI) flow, post-intervention TIMI flow, left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume (LVSDV), left ventricular mass (LVM) , left ventricular mass index (LVMI) and left ventricular ejection fraction (LVEF) were evaluated for comparison. Results: There were no differences of post-intervention TIMI flow, LVEDV, LVW or LVWI between the two groups (P>0.05). While the ratio of pre-intervention TIMI grade 3 flow, ratio of post-intervention MBG 3, and LVEF of NSTEMI group were better than those of STEMI group (P < 0.05). Conclusion: The myocardial reperfusion and left ventricular function of NSTEMI patients were prior to STEMI patients undergoing early coronary intervention. Related Articles | Metrics

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Value of MELD,MELD-Na and SOFA score for Prediction of Liver Failure 2012, 40 (9):  902-904 .  Abstract ( 941 )   PDF (165KB) ( 2711 )   Abstract Objective：To investigate the value of model for End-stage Liver Disease (MELD) score , MELD with incorporation of serum sodium(MELD-Na)score and the sequential organ failure assessment (SOFA) score for evaluation of prognosis of liver failure. Methods: A total of 290 consecutive patients with liver failure were included in the study and divided into two groups according to the prognosis .The MELD, MELD-Na and SOFA were calculated respectively and comparative analysis were performed . Areas under the receiver operating characteristic curve (AUC-ROC) of MELD , MELD-Na and SOFA were used to assess the prognosis of patients with LF . Results: The mortality of LF was 50.3% in this study. MELD, MELD-Na and SOFA were significantly higher in the death group than in the survival group (26.1±8.0 vs 20.2±5.0,33.1±12.4 vs 23.8±7.8, 9.7±2.9 vs 6.9±1.7, all p < 0.01). The area under curve （AUC）values generated by the ROC curves for SOFA score were higher （AUC=0.805）than those of MELD-Na score (AUC=0.732) and MELD score (AUC=0.714) respectively. The cut-off scores of three systems were 7.50, 28.14, 25.01 respectively, which could discriminate higher and lower mortality accurately. Conclusion：MELD,MELD-Na scores and SOFA have appreciable value to evaluate the prognosis of LF patients. SOFA is more accurate than MELD and MELD-Na scores in assessing prognosis of LF patients. Key words：liver failure1 prognosis2 MELD3 MELD-Na4 SOFA5 Related Articles | Metrics

实验研究

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Downregulation or absent of TNFSF15 in Ovarian Cancer Is a Pre-requisite for Tumor Neovascularization 2012, 40 (9):  908-911 .  Abstract ( 971 )   PDF (221KB) ( 2358 )   Abstract Objective: To study the expression of tumor necrosis factor superfamily-15 (TNFSF15) in human ovarian cancer specimens, and it’s relationship with the clinical staging. Furthermore, we mean to study the effects of TNFSF15 on tumor angiogenesis and growth in vivo. Methods: The expression of TNFSF15 in ovary tissue specimens (12 normal and 94 ovarian cancer) were detected by immunohistochemistry assay. We used recombinant TNFSF15 to treat ID8-bearing C57BL/6 mice via intraperitoneal injection or used TNFSF15-shRNA to knock down its expression in tumor microenvironment, and then the influences on tumor angiogenesis and growth were assessed. Results：Compared to the normal ovary tissues, the expression of TNFSF15 was low or absent in the cancer specimens（P<0.001）and TNFSF15 levels are inversely correlated to the clinical stages of human ovarian cancer（P<0.005）. Using a mouse syngeneic tumor model, we demonstrated that recombinantNFSF15 markedly inhibited the growth of ID8 tumors（P<0.05）; on the other hand, TNFSF15-shRNAadministration could accelerate the growth of ID8 tumors（P<0.05）. Conclusion: TNFSF15 downregulation or absent in ovarian cancer microenvironment is a pre-requisite for tumor neovascularization and tumor growth. Related Articles | Metrics

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Cloing human nanog-delt48 gene and constructing its eukryotic expression vector 1, 1, 2012, 40 (9):  912-915 .  Abstract ( 923 )   PDF (193KB) ( 2384 )   Abstract: 【Objective】: Construct the eukaryotic expression vector of pcDNA5/ FRT-nanog-delta48 and detect its expression in liver cancer cells 7721.【Method】: Clone the alternatively-spliced variant of nanog (nanog-delta48 )full-length coding sequence by RT-PCR, then insert the right PCR product into pMD18-T vector, after DNA analysis, subclone the correct identification into pcDNA5/FRT. The eukaryotic expression vector of pcDNA5/FRT-nanog-delta48 was transiently transfected into human liver cancer cell 7721 by liposome mediated, then identify its expression by RT-PCR and assess its proliferation by MTT.【Result】:The full-length coding sequence of human nanog-delta48 was cloned succefully.The eukaryotic expression vector of pcDNA5/ FRT-nanog-delta48 was proved to be constructed successful by DNA analysis, and it overexpressed in 7721 cells. MTT result showed cell proliferation increased after transfection.【Conclusion】:The alternatively-spliced variant of nanog(nanog-delta48) has similar activity to that of the full length version. It will lay the foundation for the further study of its own functions and the relationship with nanog of full-length Related Articles | Metrics

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The protect effect of olmesartan medoxomil on chronic heart failure mice 2012, 40 (9):  916-918 .  Abstract ( 1126 )   PDF (237KB) ( 2590 )   AIM : To investigate The protect effect of olmesartan medoxomil on chronic heart failure mice METHODS: C57 mice were divided into sham operation group (SHAM group), chronic heart failure group (CHF group) and olmesartan medoxomil treatment group (OLM group). Experimental CHF models were established by coronary artery ligation, in which OLM group fed with a daily dose of 10mg/kg. The heart rate, blood pressure, plasma and cardiac angiotensin Ⅱ levels; Real-time PCR detection of cardiac AT1R, type Ⅰ, Ⅲ collagen expression,and AZAN staining of cardiac pathological changes were determined after 4 weeks,．RESULTS: Compared with SHAM group, systolic blood pressure, plasma and cardiac angiotensin Ⅱ levels, AT1R and Ⅰ, Ⅲ type collagen expression were increased in CHF group (P <0.05), while in OLM Group ,they were significantly lower than that in CHF group except for the plasma angiotensin Ⅱ levels (P <0.05). AZAN staining of myocardial infarction in the CHF group were blue-stained , the myocardial fibers were disappear and replaced by fibrous connective tissue, OLM Group also can see the blue-stained fibrous connective tissue, but the area is reduced compared with the CHF group. CONCLUSION: Chronic heart failure can activate the intracardiac renin-angiotensin system and leading to heart damage,olmesartan medoxomil can protect heart by inhibiting the effects of angiotensin Ⅱ. Related Articles | Metrics

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Effect of Tongjing Bushen Recipe on Oxidative Stress of Alzheimer’s Disease Model Rats 2012, 40 (9):  919-922 .  Abstract ( 873 )   PDF (226KB) ( 2343 )   Abstract Objective: Observe the effect of Tongjing Bushen recipe on oxidative stress of Alzheimer’s Disease model rats induced by Al. Methods: Chose 84 healthy SD rats, 3-month old, randomly divided them into Control Group(Group C), Model Group ( including low-dosed Al feed group—Group M1, middle-dosed Al feed group—Group M2 and high- dosed Al feed group—Group M3) and Chinese-medicine-recipe Group (including low-dosed group—Group N1, middle-dosed group—Group N2, high- dosed group—Group N3).There were 12 rats in each group. The rats in model groups were fed with conventional feed mixed with A1C13?6H2O.The rats were exposed to this feed for 3 months, and then made into AD animal model. Determine the learning and memory ability of rats with Y-electric. The escaping latent periods of rats in Group M were obviously prolonged, the escaping correct rates of rats in Group M obviously descended. The AD animal model was proved to be successful. The rats in Chinese medicine group were fed with high-dosed Al feed and fill the stomach by Tongjing Bushen recipe. After the experiment, the rats were taken blood from their right ventricles, serums were separated and kept in proper place. Took their brains quickly and made brain homogenate. Tested the activities of SOD and GSH-PX and the level of MDA in brain tissues and serum. Results: Compared with the rats in Group C, The activities of SOD and GSH-PX in the brain tissues and serum in the rats of Group M2 and Group M3 were strikingly decreased (P<0.01), the content of MDA was strikingly increased (P<0.01). Compared the rats in Group M1 with the rats in Group C and also compared the rats in Group M2 with the rats in Group M3, each index had no striking difference (P>0.05).Compared with the rats in Group M3, the activities of SOD and GSH-PX of the rats Group N2 and Group N3 strikingly increased (P<0.01), and the content of MDA strikingly decreased (P<0.01). Compared the rats in Group M3 with the rats in Group N1 and also compared the rats in Group N2 with the rats in Group N3, each index had no striking difference (P>0.05). Conclusion: The excessive intake of Al causes the rats to be in the state of intense OS. The Tongjing Bushen recipe strikingly increased the rats' antioxidant ability and protected their central nervous system.group, middle-dosed Al feed group and high- dosed Al feed group) and Chinese-medicine-recipe Group (including low-dosed group, middle-dosed group, high- dosed group).There were 12 rats in each group. The rats in model groups were fed with conventional feed mixed with A1C13?6H2O.The rats were exposed to this feed for 3 months, and then made into AD animal model. The rats in Chinese medicine group were fed with high-dosed Al feed and fill the stomach by Tongjing Bushen recipe. After the experiment, the rats were anesthetized, open their chests, took blood from their right ventricles, serums were separated and kept in proper place. Took their brains quickly and made brain homogenate. Tested the activities of SOD and GSH-PX and the level of MDA in brain tissues and serum. Results: Compared with the rats in Control Group, The activities of SOD and GSH-PX in the brain tissues and serum in the rats of middle- and high- dosed Al feed group were strikingly decreased (P<0.01), the content of MDA was strikingly increased (P<0.01). Compared with the rats in high-dosed Al feed groups, the activities of SOD and GSH-PX of the rats in model- and high- dosed Chinese medicine groups strikingly increased (P<0.01), and the content of MDA strikingly decreased (P<0.01) Conclusion: The excessive intake of Al causes the rats to be in the state of intense OS. The Tongjing Bushen recipe strikingly increased the rats' antioxidant ability and protected their central nervous system. Related Articles | Metrics

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The histological study of Mesenchymal Stem Cells on tendon-bone healing in bone tunnel 2012, 40 (9):  923-925 .  Abstract ( 892 )   PDF (250KB) ( 2396 )   [Abstract] Objective To research the effect of Mesenchymal Stem Cells（MSCs）on tendon-bone healing in bone tunnel . Methods Anterior cruciate ligament reconstruction with semitendonous tendon was done in knee joints of New Zealand rabbits by suspend fixation model. Mesenchymal Stem Cells were implanted in bone tunnel in experimental group and only operation in control group. Specimens were collected 4 weeks, 8 weeks and 12 weeks after surgery. Sections were stained with HE, MASSON . Special changes of interface tissue between bone tunnel and tendon graft were observed and informations of healing between tendon and bone tunnel were analyzed. Results In MSCs group abundant penetrating Sharpey fibers were appeared at 8weeks and Sharpey fibers were appeared at 12 weeks in control group，direct connection can be seen in MSCs group. Conclusion MSCs can accelerate tendon healing in bone tunnel. Related Articles | Metrics

短篇论著

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The in-vitro Interferon-Gamma Release Assay T-SPOT.TB for the Diagnosis of Tuberculosis wei Guo LIU 2, 2012, 40 (9):  926-928 .  Abstract ( 2241 )   PDF (167KB) ( 2501 )   【Abstract】 Objective To evaluate the in-vitro Interferon-Gamma Release Assay T-SPOT.TB for the Diagnosis of Tuberculosis . Methods The peripheral blood mononuclear cells(PBMC) were collected from the patients with tuberculosis(tuberculosis group,n=36) ,patients with extrapulmonary tuberculosis,( extrapulmonary tuberculosis group,n=18),and healthy controls(healthy control group,n=31).The PBMCs were co-cultured with different antigens: ESAT-6 and CFP-10.The levels of interferon-Gamma were detected by ELISPOT ,and the results were compared to those with tuberculin skin test(TST)、TB-AB、ESR and ADA. Results The T-SPOT.TB assay showed positive results in 46 patients with tuberculosis and the sensitivity was 92.6%. There was significant difference of the T-SPOT.TB results in patients with tuberculosis and healthy controls, and the same in patients with extrapulmonary tuberculosis and healthy controls（P＜0.05）. There was no significant difference of the T-SPOT.TB results in patients with tuberculosis and patients with extrapulmonary tuberculosis（P＞0.05）. There was no significant difference of the TST results among the three groups(P＞0.05).The specificity of T-SPOT.TB(93.5%) was higher than TST(54.8%) in healthy controls, the difference was significant（P＜0.05）. The levels of TB-AB、ESR and ADA was higher in patients with tuberculosis and patients with extrapulmonary tuberculosis than in healthy controls, the difference was significant（P＜0.05）.Conclusions The T-SPOT.TB has highly specificity in diagnosis of tuberculosis and is better than TST. the significance is higher if combined T-SPOT.TB with TB-AB、ESR and ADA . Related Articles | Metrics

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The Establishment Determination Method for Milk Allergy Specific Antibody 2012, 40 (9):  930-931 .  Abstract ( 1239 )   PDF (150KB) ( 2633 )   Abstract Objective: to establish a double antigen sandwich enzyme linked immunosorbent assay (ELISA) to detect the specific antibody in milk allergic patient serum. Methods: main components of milk allergen were used to coat microwell plate and prepare enzyme conjugate antigen by labelling horseradish peroxidase (HRP). The specific antibody in test serum was combined with coating antigen and enzyme conjugate antigen simultaneously to form the double antigen sandwich compound. After enzyme catalyzed coloration and reaction termination, the absorbance at 450 nm (A450) was read and results were judged by setting 2.1 A450 of negative control as the cut off value. Results: the coefficient of variations (CV) in the group and between groups of the developed method were 3.7%-13.6%, and its sensitivity, specificity, accuracy were 91.7%, 94%, 93.3% respectively. Conclusion：the double antigen sandwich ELISA established in this study can detect the specific antibody in doubtful milk allergic patients serum，showing its feasibility in vitro diagnosis of milk allergy. Related Articles | Metrics

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Clinical Significance of Pathological Diagnosis in Patients with Uterine Leiomyomatosis with Unusual Growth Patterns 2012, 40 (9):  932-934 .  Abstract ( 1005 )   PDF (275KB) ( 2687 )   To investigate the clinical value of pathological diagnosis on uterine leiomyoma with unusual growth patterns (special histology and growth pattern) to guide the appropriate surgical procedures and treatment. Methods: There were three groups in this study. Group A were 110 patients with unusual types of uterine leiomyoma treated in our hospital during 1982 – 1987. Group B were 41 cases with special types of uterine leiomyoma treated in our hospital during 2007 – 2010. Group C were control group included 41 cases of ordinary uterine leiomyoma randomly selected over the same period. The patient age, surgical modes, myoma recurrence rate and malignant transformation rate were retrospectively analyzed and compared between three groups by 1 to 5 years follow-up. Results: There were no significant differences in the patient age, surgical modes and recurrence rate between patients of three groups. No malignant transformation was found in three groups. Conclusion: The special types of uterine leiomyoma have benign manifestations. The pathological diagnosis of undetermined malignant potential uterine leiomyoma should becautious. Pathologist and clinical doctors should avoid understanding deviation and excessive therapy. Close follow-up is needed after surgery. Related Articles | Metrics

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Effect of pneumo-peritoneum with CO2 lasting long time course on correlation of PaCO2 and PetCO2 during Urological laparoscopic surgery ,Guo-Yi LV 2012, 40 (9):  934-935 .  Abstract ( 931 )   PDF (155KB) ( 2494 )   Objectives：To appraise whether arterial partial pressure of end—tidal carbon dioxide(PetCO2) can accurately reflect the value of arterial partial pressure of carbon oxide(PaCO2) during prolonged laparoscopic procedure and the correlation between them. Methods：40 ASA physical status I and II patients，without cardio-pulmonary dysfunction preoperatively，scheduled for elective urologic laparoscopic procedures, pneumo-peritoneum course lasted over 3 hours. After conventional induction and tracheal intubation, the values of PetCO2 and PaCO2 were observed and recorded at post-intubation, at 1, 2 and 3 hr. point post-pneumoperitoneum, right half hr. at the end of pneumo-peritoneum. Then, regression formula was built in correlation between PetCO2 and PaCO2 at different time-point. Results: A remarkable positive correlation between PetCO2 and PaCO2 was found at the point of post-intubation, 1 and 2 hr. post-pneumoperitoneum, and half hr. at the end of post-pneumoperitoneum（r=0.966，P<0.01）. But as time prolonged, the correlation between PetCO2 and PaCO2 changed badly, there was an increase in the values of both PetCO2 and PaCO2, but the increase range was much higher in PaCO2 than that of in the PetCO2 （r=0.585，P< 0.01）.Conclusions: There was a better correlative relationship in monitoring results between PetCO2 and PaCO2 during the laparoscopic procedure of urology under pneumoperitoneum with carbon oxide, which can correctly reflect the tendency of change of PaCO2 under the condition of pneumoperitoneum, that can meet clinical use, but the D-value increases and correlation went to bad between PetCO2 and PaCO2 as pneumoperitoneum duration prolonged. So,besides conventional monitoring PetCO2, respiratory parameters should be modulated in time according to the results of blood gas analysis in order to avoid hypercapnia when pneumoperitoneum duration lasted over 2 hours. Related Articles | Metrics

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The research of lower limb strength changes impacted by history of falls 2012, 40 (9):  938-939 .  Abstract ( 932 )   PDF (145KB) ( 2520 )   Abstract Objective : To study lower limb strength changes of fallers in further to analyze fall related factors and prevent fall. Method : Isokinetic muscle testing was performed in 14 subjects with a history of one or more falls in the prior year (i.e. fallers) as well as a control group of 58 non-fallers. Peak torque (PT) and power (POW) were recorded at two velocities (60 degree/s and 120 degree/s) on a Biodex Isokinetic Dynamometer for four muscle groups which include knee extensors, knee flexors, ankle plantar flexors and ankle dorsiflexors. All parameters of fall group were compared with control group. Results: No significant difference was observed in PT and POW of knee extensors and flexors in two groups (p>0.05). PT and POW of ankle dorsiflexors in fall group was significant smaller than non- fall group at 120 degree/s (p<0.05). PT of ankle plantar flexors in fall group was significant weakness than non- fall group at 60 degree/s (p<0.05). Conclusion : Isokinetic muscle testing can be applied to older people fall risk protocol. It can provide referencing data for fall risk evaluation as well as the major training item. Related Articles | Metrics

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Relationship Between Uric Acid and Coronary Heart Disease and it’s Risk Factors by SYNTAX score 2012, 40 (9):  940-941 .  Abstract ( 806 )   PDF (129KB) ( 2322 )   Related Articles | Metrics

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Retrospective comparison of total hip arthroplasty through a minimally invasion versus standard posterolateral approach 2012, 40 (9):  942-943 .  Abstract ( 836 )   PDF (151KB) ( 2393 )   【Abstract】0bjective To evaluate the clinical outcome of total hip arthroplasty through a minimally invasion and summarize the surgical experience .Methods thirty cases of THA were performed through minimally invasive anterial surgical approach(MIS group)．Among them，there were 22 males and 8 females，the mean age was 64.2 years and the mean BMI was 23.4kg／m2 and the mean Harris score was 31.9．For comparison，another 30 cases of THA were performed through a standard posterolateral approach at the same period(standard group)．the mean age，BMI and Harris score were similar to the MIS group．Operation time，blood loss，incision length，complication，postoperative VAS pain score，postoperative hospitalization，functional recovery were analysed and compared between the above two groups．Results These patients were followed up for average one year，no infection，fracture and neurovascular injury occurred in both groups．There were one skin late healed 、four deep venous thrombosis and three dislocation in standard group．There were big significant differences regarding operation time and incision length in the two groups．There were significant differences regarding the complications，postoperative VAS pain score，postoperative hospitalization，functional recovery of one month and six months, but there were no significant differences regarding the functional recovery of one year after operation． Conclusion THA through a minimally invasive anterial surgical approach is an effective and reproducible operative technique．The advantages of MIS THA includes less postoperative pain，shorter post-operative hospitalization and faster rehabilitation．．But the selection of suitable patients for mini-incision THA should be noticed. Related Articles | Metrics

临床论丛

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Investigate the reasons and treatment methods for the mechanical complications of Ankylos implant abutment 2012, 40 (9):  946-947 .  Abstract ( 870 )   PDF (153KB) ( 2569 )   Abstract Objective: Investigate the reasons and treatment methods for the mechanical complications of Ankylos implant system’s abutment . Methods: From 2005 to 2010, the incidence of all kinds of abutment’s mechanical complications and their treatments were summarized in this paper. Analyse the reasons and characteristics of the abutment’s mechanical complications of the Ankylos implant system with statistics. Explain the methods and the matters needing attention of dealing with these complications. Results: There were significant differences with the incidence of abutment’s mechanical complications ,male more than female(2.82% vs 0.67%，P＜0.05),posterior more than anterior zone（2.53% vs 0.80%，P＜0.05）,Balance posterior abutments more than Standard abutment（4.32% vs 0.44%，P＜0.05） and Balance anterior abutments （4.32% vs 0.80%，P＜0.05）.All the mechanical complications have been solved,and no abnormal symptoms have been found in the follow-up period. Conclusions: There are uniqueness of the reasons and treatment methods for the mechanical complications of Ankylos implant abutment. Standard abutment should be preferred in the posterior zone of male. The proposed methods are simple and effective, no damage to implant’s internal structure, no increase instrument and equipment, patients satisfied with a median follow-up, are worthy to be popularized in the clinical work. Related Articles | Metrics

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The new birth for delivery mode influenced by the way 2012, 40 (9):  948-949 .  Abstract ( 795 )   PDF (186KB) ( 2578 )   Abstract Objective :explore the new mode to promote midwifery vaginal delivery, reduce cesarean section rate was the effect. Methods: select in October 2008-February 2011 in our antenatal examination and in our delivery of the pregnant women 270 cases as the observation group implementing new birth, 250 cases of the model was the implementation of the original midwifery mode. Comparing the two group of delivery mode, total process and fetal weight. Results:two groups in childbirth, total process time and way fetal weight are significant difference (p < 0.05). Conclusion:the new model can promote midwifery vaginal delivery, reduce cesarean section rate was, shorten the process, reduce the incidence of great son. Related Articles | Metrics

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Minimal Invasive Fixation for Treatment Of Adults' Midshaft Clavicle Fracture With Herbert Screw 2012, 40 (9):  949-950 .  Abstract ( 801 )   PDF (178KB) ( 2390 )   [Objective] To discuss the operation techniques and therapy effects in treating adult mid-shaft fractures of clavicle with cannulated Herbert screws. [Methods] Between 2009.9 and 2010.12, twenty seven patients with mid-shaft clavicle fractures were recruited from the Third affiliated Hospital of Suzhou University, Changzhou. The twenty seven patients were made up of 20 men and 7 women with an average age of 34.8 years. Mid-shaft fractures were fixed by cannulated Herbert screws through minimal invasive incision. The mean length of incision was 2.0cm. [Results] All 27 patients were followed up for 14.7 months (range10 to 18 months).All fractures of this group achieved bone union at average 11.3 weeks） postoperatively. No severe complications, such as incision infections and neurovascular injuries, occurred in this group. No delayed union, nonunion and Screw breakage were found in follow-up. The mean UCLA score was 34.3 at the end time of follow-up. [Conclusion] This study showed that midshaft clavicle fracture fixed with cannulated Herbert screw minimal invasively obtained good immobilization, excellent bone union and less complications. Moreover, it has advantages of simple operation techniques and protect blood supply. It may be a better choice to treat midshaft clavicle fracture for young adults and women who love beauty. Related Articles | Metrics

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A case-control study on risk factors of gestational diabetes mellitus 2012, 40 (9):  951-952 .  Abstract ( 959 )   PDF (145KB) ( 2565 )   Abstract Objective : To investigate the risk factors of gestational diabetes mellitus and provide the basis for intervention. Methods: The method of 1:2 matched case-control study was used to study the risk fators of gestational diabetes mellitus.The chi-square test was used in univariate analusis and Logistic regression was used in multivariate analusis. Results: The odd ratio and 95% confidence interval of education levels, pre-pregnancy BMI, pregnancy waist, pregnancy waist hip ratio, family history of diabetes and family history of hypertension were 1.829 (1.250-2.057), 1.589 （1.247-1.900）, 2.197 （1.110-4.349）, 2.238 （1.798-3.920）, 3.015 （1.851-5.192）, 2.824（1.621-4.596）.Conclusions: The risk factors of gestational diabetes mellitus were education levels, pre-pregnancy BMI, pregnancy waist, pregnancy waist hip ratio, family history of diabetes and family history of hypertension. So people with those should be intervened firstly. Related Articles | Metrics

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Application of transesophageal echocardiography on minimally invsive surgical closure of ventricular septal defect 2012, 40 (9):  954-955 .  Abstract ( 983 )   PDF (248KB) ( 2537 )   Related Articles | Metrics

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The predictive value of H-FABP for the acute pulmonary embolism patients in recent prognosis 2012, 40 (9):  955-956 .  Abstract ( 908 )   PDF (149KB) ( 2366 )   Objective： To evaluate the predictive value of heart -type fatty acid-binding protein (H-FABP) for the acute pulmonary embolism patients in recent prognosis . Methods: From August 2009 to July 2011, 87 patients with acute pulmonary embolism were enrolled in people's hospital of Xingtai . After be in hospital the H-FABP level of these patients were determined. According to the blood H-FABP determination values ,87 cases were divided into two groups. Positive group: H-FABP ≥10 ng/mL (n = 21), negative group: H-FABP < 10 ng/mL (n = 66). And we have compared the different rates of adverse events between the two groups in syncope, shock and death .At the same time we have respectively compared the different blood H-FABP levels between the two groups when these clinical adverse events happen or not. Result: In the positive group it has higher incidence in Syncope, shock ,right heart dysfunction, fibrinolytic therapy,and mechanical ventilation. These all have statistically significant (P < 0.05). Total 2 deaths had occurred in H-FABP positive group during the 30 days. H-FABP level in the group with clinical adverse events（15.23 ng/mL±2.92 ng/mL）is significantly higher than the group without adverse events（5.49 ng/mL±3.36 ng/mL）.The result has statistically significant differences (P < 0.01). Conclusion: H-FABP increase is the risk factor in the recent death of acute pulmonary embolism patients and indicates poor prognosis . It provides effective risk stratification for the acute pulmonary embolism patients. Related Articles | Metrics

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Comparison of effects of dezocine and fentanyl preventing the incidence of agitation in adult patients during recovery period after thoracic surgery 2012, 40 (9):  957-958 .  Abstract ( 1046 )   PDF (156KB) ( 2389 )   【Abstract】 Objective To compare the effects of usual clinical doses of dezocine and fentanyl preventing the incidence of agitation in adult patients during recovery period after thoracic surgery. Methods Eighty ASA I-Ⅱ patients scheduled for elective thoracic surgery under general anesthesia were randomly，double blindedly divided into two groups with 40 cases each.The patients were administered intravenously dezocine 10mg(group D)and fentanyl 0.1mg(group F) 20min before the end of surgery. The RSS agitation scale, the incidence of agitation,wake-up time and adverse events were assessed．Results The scale and incidence of agitation was no significant difference between group D and F(P>0.05).Compared with group F,the wake-up time of group D was shorter than F significantly(P<0.05),and the incidence of adverse events of group D was also lower than F(P<0.05).Conclusion Usual clinical doses of dezocine and fentanyl can prevent the incidence of agitation during recovery period after thoracic surgery effectively.However,the incidence of adverse events of dezocine is lower than fentanyl. Related Articles | Metrics

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Short and medium term clinical observe of implant prosthesis to the edentulous jaw patients resulted by severe periodontitis 2012, 40 (9):  960-961 .  Abstract ( 900 )   PDF (165KB) ( 2611 )   Objective: To investigate clinical effects of implant prosthesis to the edentulous jaw patients resulted by severe periodontitis. Methods: 15 edentulous jaw patients resulted by severe periodontitis were treated by implant-supported complete overdenture,and received clinical examination and x-ray examination 1 year and 2 years later after restoration. Results: All implants within follow-up period were steady ,and belong to healthy implants.Conclusion: For edentulous jaw patients resulted by severe periodontitis, the therapeutic method of Straumann implant-supported overdenture has reliable effect, but long term effect should be observed further. Related Articles | Metrics

综述与讲座

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Research progress of the etiology related to urolithiasis longlong FU1, 2012, 40 (9):  968-970 .  Abstract ( 978 )   PDF (179KB) ( 2852 )   Urolithiasis is the common health problem. A considerable percentage of patients experience recurrent kidney stones with pain and urinary tract infections and even cause renal insufficiency. The high prevalence and the recurrence rate are the key problem to the treatment. It is a multifactorial disease. Research the etiology of the urolithiasis, prevention and control the disease become the hot issues. The paper to review the new research of the risk factor, such as: gender, age, environment, metabolism, and so on. Related Articles | Metrics