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15 October 2012, Volume 40 Issue 10 Previous Issue    Next Issue
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论著
实验研究
短篇论著
临床论丛
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论著

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Study of Rock2 on the Proliferation and Apoptosis of Hepatocellular Carcinoma Cells 2012, (10):  979-982 .  Abstract ( 781 )   PDF (188KB) ( 2366 )   Objective: To investigate the effects of Rock2 on proliferation and apoptosis of hepatocellular carcinoma Huh-7 and HepG2 cell lines. Methods: The experiment was divided into untreated group, non-targeting group, PBS group and shRock2 group. Hepatocellular carcinoma Huh-7 and HepG2 cell lines were transfected with shRock2. Reverse transcriptase polymerase chain reaction and Western blot assays were used to detect the Rock2 mRNA and protein levels, respectively. Huh-7 and HepG2 cell proliferation was measured by MTT assay. Change of cell cycle was detected by PI staining method through flow cytometry and earlier apoptosis was demonstrated by Annexin V apoptosis kit. Results: The Rock2 mRNA and protein levels both noticeably decreased in shRock2 transfected Huh-7 and HepG2 cells. After the silence of Rock2, cell proliferation was blocked in the shRock2 cells compared to the control groups (P<0.01). Knockdown of Rock2 promoted cell-cycle arrest at the G0/G1 phase. But the percentage of cells in S phase and G2/M decreased compared to the control groups（P<0.01）. The earlier apoptosis rate of shRock2 Huh-7 and HepG2 cells increased. This value was significantly higher than those of the control groups (P<0.01). Conclusion: Knockdown of Rock2 led to decreased proliferation, increased apoptosis in HCC cells and Rock2 may be a new target for HCC gene therapy. Related Articles | Metrics

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The mRNA Expression of IFN-γ and IL-4 are not Correlated with their DNA Methylation Status in ITP Patient 2012, (10):  983-986 .  Abstract ( 928 )   PDF (171KB) ( 2249 )   Objective: DNA methylation had been researched in many autoimmune diseases. Immune thrombocytopenia (ITP) is an organ-specific autoimmune disorder characterized by accelerated platelet destruction and bleeding symptoms. DNA methylation negatively regulates the gene expression. The aim of the current study was to investigate the relationship between the transcription levels of the IFN-γ and IL-4 and the methylation status of those gene promoters in ITP patients. Methods: The mRNA expression levels and DNA methylation of the IFN-γ and IL-4 were detected by the real-time quantitative PCR and bisulfite genomic sequencing, respectively. The relationship between CpG methylation and the mRNA levels of those genes were determined. Results: When compared with the healthy controls, the IFN-γ mRNA levels of ITP patients were significantly higher (1.86±0.29 vs 0.83±0.14, P<0.05). The levels of IL-4 mRNA transcripts were notably lower in ITP patients (0.78±0.22 vs 1.45±0.40, P<0.05). Most importantly, the Th1/Th2 (IFN-γ/IL-4) was remarkably higher in ITP patients than that of the controls (7.11±0.60 vs 3.12±1.88, p<0.01). There were no closely relationship between the mRNA levels of IFN-γ and IL-4 genes and the methylation status of those gene promoters. Conclusion: The ITP patients were in the Th1 polarization. The mRNA expression of IFN-γ and IL-4 were not associated with the DNA methylation status of those genes in ITP patients, indicating that the IFN-γ and IL-4 mRNA were not controlled by the DNA methylation of those genes. Related Articles | Metrics

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Characteristics and risk factor of hyperuricemia among 946 coal industry employee in Tangshan city 2012, (10):  987-989 .  Abstract ( 830 )   PDF (181KB) ( 2254 )   Abstract Objective: To analyze the characteristics and risk factor of hyperuricemia (HUA) among coal industry employee. Methods: The survey population in this study is 946 coal industry employees in Tangshan city. Inquiring about the general situation, personal habits, family history of disease, and determination of height, body mass and blood pressure. We analysis indicators of blood uric acid, blood glucose testing, total cholesterol (TC), Triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C). Results: The detectable rate of HUA was 10.7%. There was a remarkable statistical difference of BMI, TG, TC, SBP, DBP in subjects without HUA and with HUA (P<0.05). There is highly rate of men, obesity, current smoking, current drinking, history of HP, HP, dyslipidemia in subjects with HUA (P<0.05). Multivariate analysis showed that sex, obesity, dyslipidemia, hypertension are risk factories of HUA. Conclusions: There was a highly detectable rate of HUA in coal industry employee. Abnormal lipid metabolism, HP and unhealthy lifestyle habits maybe its causative factor. Related Articles | Metrics

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Expression and significance of VEGF-C and VEGFR-3 in colorectal carcinoma 2012, (10):  990-994 .  Abstract ( 793 )   PDF (234KB) ( 2207 )   Abstract Objective: To investigate the relationship between the expression of vascular endothelial growth factor-C (VEGF-C), vascular endothelial growth factor receptor-3 (VEGFR-3) and lymphangiogenesis, lymph node metastasis in colorectal cancer. Methods: Fifty colorectal cancer tissues and twenty colorectal adenoma tissues were selected randomly, meanwhile twenty normal tissues were selected as negative control group. Immunohistochemistry was used to detect the expression of VEGF-C and VEGFR-3. Lymphatic vessel density(LVD) was evaluated with lymphatic endothelium-specific marker, lymphatic vessel endothelial hyaluronan receptor-1(LYVE-1). All specimens had complete clinicopathologic data. Results: The positive rates of VEGF-C and VEGFR-3 expression in colorectal cancer were 44% and 36%, respectively. However, VEGF-C and VEGFR-3 expression were not found in colorectal adenoma tissues and normal tissues. There were significant differences in expressions of VEGF-C and VEGFR-3 proteins between patients with and without lymph node matastasis (P<0.001). There were no significant differences between gender, age, TNM stage，Dukes stage, Broder stage, histological type and invasion depth in CRC(P>0.05). LVDs were significantly higher in VEGF-C-positive and VEGFR-3-positive tumors compared with VEGF-C-negative and VEGFR-3-negative tumors(P<0.001). Univariate analysis revealed that Broder stage, VEGF-C-positive and VEGFR-3- positive were predictive factors of lymph node metastasis((P=0.024, P＜0.001, P＜0.001). Multivariate analysis revealed that the independent factors associated with lymph node metastasis were VEGF-C and VEGFR-3(P=0.001, P=0.02). Conclusions: This study suggests that the expression of VEGF-C and VEGFR-3 contributed to lymphangiogenesis and lymph nodal metastasis in colorectal cancer. VEGF-C and VEGFR-3 may serve as a new target of biological therapy in colorectal cancer. Key Words colorectal cancer, VEGF-C, VEGFR-3, lymphangiogenesis, lymph node metastasis Related Articles | Metrics

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An experimental study of siRNA targeting P75NTR to induce apoptosis of U251 glioma cells 2012, (10):  995-997 .  Abstract ( 780 )   PDF (184KB) ( 2248 )   Abstract Objective: To observe the therapeutic efficacy of siRNA silencing P75 neurotrophin receptor (P75NTR) to induce apoptosis of U251 glioma cells. Methods The siRNA fragments targeting P75NTR were transferred into glioma cell line U251. RT-PCR was used to explore the expression of P75NTR mRNA. Platelet counting method was employed to get cell growth curve, in situ apoptosis assay to detect cell apoptosis, and Western blot to proteins expression of P75NTR, pro-apoptotic gene caspase-3 and anti-apoptosis gene Bcl-2, after gene transfection 1,3,5 and 7d. Results The siRNA targeting P75NTR can decrease mRNA expression of P75NTR in U251 cell, decrease cell proliferation, and increase cell apoptosis. P75NTR and Bcl-2 protein level in RNAi group were lower than the other two groups. This trend was contrast with the caspase-3. Conclusion The gene silencing technique by siRNA targeting P75NTR can induce cell apoptosis through the mechanisms regulating the expression of apoptosis-related genes. It is expected to become a new choice for gene therapy of glioma. Related Articles | Metrics

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Analysis of the Related Factors of Lymph Nodes Metastasis in 268 Cases of Endometrial Carcinoma 2012, (10):  998-1001 .  Abstract ( 1211 )   PDF (213KB) ( 2472 )   Abstract Objective: To investigate the related clinical-pathological factors of lymph node metastasis in patients with endometrial carcinoma. Methods: A retrospective analysis on 268 patients with endometrial carcinoma from Jan. 2002 to June. 2011 were selected.The patients were classified according to the WHO Classification of Tumors of uterine corpus (2003) and the latest FIGO stage criterion. The patients were divided into lymph node metastasis positive group and negtive. The differences of the following parameters between the two groups were compared: depth of myometrial invasion, pathological type, para-uterus invasion, differential degree of endometrioid carcinoma, age, the result of abdominal cytology and serum CA125 level. Simultaneously, the correlation between the above parameters and lymph node metastasis were analyzed. Result: The lymph node metastasis rate was 10.07%(27/268), and external iliac and obturator lymph nodes were the preferred sites which took up about 81.48%. The abdominal aorta lymph node metastasis rate was 9.68%(6/62). The metastasis rates of patients with deep myometrial invasion, non-endometrioid adenocarcinoma, middle and poorly differentiation, para-uterus invasion, abnormal serum CA125 level, age≥60 were significantly higher than those of patients with non or superficial myometrial invasion, endometrioid adenocarcinoma, well differentiation, without para-uterus invasion, normal serum CA125 level and age<60(P<0.01 for all), respectively. No significantly difference was observed between patients positive and negative for abdominal cytology detection. Differentiation degree of endometrioid adenocarcinoma, myometrial invasion and serum CA125 level are correlated with lymph node metastasis by logistic regression analysis. Conclusion: Deep myometrial invasion, middle & poorly differentiation and elevated serum CA125 level are high risk factors of lymph node metastasis in endometrial cancer patients. Related Articles | Metrics

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Serum uric acid levels and related factors in elderly patients with acute coronary artery syndrom 2012, (10):  1002-1004 .  Abstract ( 715 )   PDF (156KB) ( 2235 )   Abstract Objective: To investigate the association between serum uric acid(UA)levels and different types of ACS, the severity of angiographic coronary artery stenosis and other related factors. Methods: The study group consisted of 272 consecutive patients over 75 years old with unstable angina pectoris or acute ST segment elevated myocardial infarction(STEMI) or non ST segment elevated myocardial infarction (NSTEMI) who underwent coronary angiography. Patients were divided into One, two, three-vessel coronary artery lesion group. Serum uric acid and creatinine level, as well as a fasting lipid profile and fasting blood glucose, were measured in all patients. Results: There were no significant difference in UA level among angina pectoris group, NSTEMI group and STEMI group. No difference of UA level were found among the three groups with different vessels lesion. The UA levels were significantly correlated with levels of Cr ,TG ,WBC positively and correlated with HDL-C levels negatively. Mutiple aggression analysis found that Cr、 HDL-C、 WBC were independently correlated with UA. Conclusions: UA levels were not affected by neither ACS types nor severity of coronary lesion in elderly patients over 75 years. Cr、 HDL-C、WBC were independently related factors of UA. Related Articles | Metrics

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A three-dimensional quantitative measurement related to the operation about tympana by using multi-slices spiral CT 2012, (10):  1005-1008 .  Abstract ( 744 )   PDF (231KB) ( 2296 )   Objective: To provide a valuable imaging anatomic method for operation of tympana and accurate positioning among relative structures in operation. Methods: The clinical information of 112 adult cases(224 sides)was analyzed. All the underwent CT scaning and three-dimensional image reconstruction of skull base. Axial and coronal images were reconstructed using multiplanar reconstruction technique of station and the anatomic objective structure were displayed by rotating imaging slices. The data were analyzed in statistically and compared with the published cadaver data. Results: The distance from the chochleariform process to the horizontal portion of facial nerve recess(C), the shortest distance from the pyramidal eminence to the pyramid segment of the facial canal(D), inner diameter (E)and outer diameter(F) of facial recess, inner diameter (G)and outer diameter(H) of sinus tympani, the shortest distance from the head of stapes to the horizontal portion of facial nerve recess(N), the shortest distance from the stapedial footplate to the horizontal portion of facial nerve recess(O), among the different genders have significant(P＜0.05). The left distance from the anterior wall of sigmoid sinus to the posterior wall of extemal auditory meatus(A), the distance from the lateral wall of sigmoid sinus to the surface of mastoid process(B), the vertical distance from the lateral semicircular canal to the jugular bulb(Q) are greater than right, among the different sides have significant(P＜0.05) There were no significant differences between imaging quantitative measurement and published cadaver data(P＞0.05). Conclusions: The result of three–dimensional quantitative measurement by using multiplanar reconstruction could represent a real distance of anatomic structures. It may provide a valuable method for surgical approach and accurate positioning of relative structure in operation. Related Articles | Metrics

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Proteomics-based analysis of differentially expressed proteins in the sera of stage I non-small cell lung cancer patients and healthy subjects 2012, (10):  1012-1014 .  Abstract ( 742 )   PDF (163KB) ( 2231 )   Objective: To seek differentially expressed serum proteins related to stage I non-small cell lung cancer (NSCLC). Methods: The sera of lung cancer group were collected and balanced mixed from 6 patients who were surgically and pathologically diagnosed as stage I NSCLC in Tianjin chest hospital from September 2011 to November 2011. Those of control group were collected and balanced mixed from 15 healthy subjects in the corresponding period. Two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) were employed to screen and identify differentially expressed serum proteins between the two groups. Results: Two-dimensional electrophoresis (2-DE) patterns of serum proteins with high-resolution and reproducibility were successfully obtained from sera of the two groups. The average protein spots were 330±12. One hundred and twenty eight significantly differentially expressed protein spots between the two groups were screened with PDquest software．Ten proteins were successfully identified via peptide mass fingerprinting using MALDI-TOF-MS. Among them, 7 proteins were overexpressed, including alpha-1-acid glycoprotein, C1 inhibitor, angiotensinogen, transferrin, transthyretin, inter-alpha-trypsin inhibitor heavy chain H2 and ficolin-3. Whereas three proteins were underexpressed, including fibronectin, complement C4-A and complement C3. Conclusion: Four differentially expressed proteins close related to cancer can be separated and identified initially in the sera of patients with stage I NSCLC by 2-DE and MALDI-TOF-MS, which may serve as candidate biomarkers for early detection of NSCLC. Related Articles | Metrics

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Association of endothelial nitric oxide synthase gene polymorphism with essential hypertension liangrong liang rong 2012, (10):  1015-1018 .  Abstract ( 836 )   PDF (226KB) ( 2286 )   Objective To investigate the association between G894T and 27bp varible number of tandem repeats(VNTR) polymorphism of endothelial nitric oxide synthase(eNOs) gene and essential hypertension(EH) Methods The PCR-RFLP method was used to detect genetypes of eNOS gene exon 7th 894 site and intron 4 th VNTR in350 patients with EH and 150 controls. Questionnaire refered to smoking,drinking, body mass index,etc was administered.Results There was no significant difference in frequencies of T allele and GT+TT genotypes in hypertension group than in control. The frequencies of a allele and aa+ab genotypes were significantly higher in hypertension group than in control. There was no significant synergistic effects of eNOS gene exon 7th 894 site and intron 4 th VNTR in EH.Conclusion The polymorphism of eNOS G894T was not associated with hypetension,while the polymorphism of intron 4 th VNTR was associated with hypertension,there was no significant synergistic effects. Related Articles | Metrics

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Effect of dezocine in induction period on stress response and recovery profiles for glottis operation Ai-Feng LIU,Guo-Yi LV 2012, (10):  1019-1021 .  Abstract ( 840 )   PDF (184KB) ( 2228 )   [Abstract]Objective To observe the influence of dezocine given intravenously in induction period on stress response, haemodynamics, recovery profiles for glottis operation by retaining laryngoscope. Methods 48 adult patients, ASA I-II, undergoing glottis operation under retaining laryngoscope，were randomly divided into group C and D(n=24). Group C received 1ml of saline; group D dezocine 5mg/1ml. Mean arterial blood pressure (MAP) and heart rate (HR) at different time during operation were recorded, the extubating time( duration from the end of operation to extubation), agitation score at extubation and the Ramsay score of sedation after 5 min of extubation also recorded. Blood sample was drawn pre-anesthsia and at the end of operation, the value of cortisol in the plasma was determined. Results：MAP in group D was a little lower than that of group C(P<0.05), HR a little higher than that of group C(P<0.05) at 5 min later extubation; There was no significant differences in both extubating time and the Ramsay score of sedation at 5 min later extubation between two groups (P>0.05). Agitation score in group D was much lower than that of group C during extubation（P<0.05）.The increasing value of cortisol in group D was a little lower than that of groupC (P<0.05). Conclusion 5mg of dizocine at pre-operation intravenously may decrease patient’s stress response peri-operatively, agitation after extubation, and no prolong the recovery time, also improve the recovery qualities, who undergoes glottis surgery by retaining laryngoscope. Related Articles | Metrics

实验研究

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An Experimental Study On the Restorative Effect of Coralline Hydroxyapatite versus Beta-tricalcium Phosphate Filled the Bone Defects around Implants 2012, (10):  1026-1029 .  Abstract ( 886 )   PDF (219KB) ( 2538 )   Objective: To investigate the restorative effect of coralline hydroxyapatite and beta-tricalcium phosphate on the defect healing around implants. Methods: All mandibular premolars and 1st molars were extracted bilaterally in six Beagle dogs. After a healing period of three-month, four experimental canals were prepared on each side of the mandible. The margin of the canal was further drilled to create the 1-1.25mm circumferential and 5mm deep bone defect around the coronal portion. Then, four implants were inserted in extraction sites on each side. The standardized bone defects were filled with blood clot, CHA or with β-TCP，respectively. All implants were non-submerged. Each two dogs were sacrificed at weeks 4, 12 and 16 following implant placement, and the specimens were prepared and stained with trinitrophenol fuchsin for histological and histomorphometric analyses to acquire the data about the distance between the most coronal level of bone-to-implant contact and the bottom of the surgically created bone defect(B-D), and the data about the bone-to-implant contact (BIC%) within the defect area. Values for newly formed bone area percentage (NFB%) and remaining bone substitutes percentage (RBS%) were additionally calculated for all biomaterial-grafted samples utilizing the Image-Pro Plus 6.0 software. Results: No implant was loosen or lost after installation, the peri-implant soft tissue was indicated no inflammation. At weeks 4 and 12, the differences in B-D and BIC% were not statistically significant between three treatment groups(P>0.05); the B-D, BIC% values for β-TCP –grafted group were significantly higher than for CHA-grafted group at week 16(P<0.05). There were statistically significant differences in RBS% within bone defect area between CHA-grafted and β-TCP –grafted groups at week 12、16，and the NFB% value of β-TCP –grafted group was shown significantly greater than that of CHA-grafted group(P<0.05).Conclusion: Bone regeneration within defects surrounding the implant could be accelerated with the use of β-TCP than CHA, it was suggested that the application of β-TCP was one more predictable treatment with respect to restoring bone defects around implants. Related Articles | Metrics

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Inhibitory Effect of Berbamine on Proliferation of Rat Osteosarcoma Cells UMR-106 2012, (10):  1030-1033 .  Abstract ( 810 )   PDF (220KB) ( 2317 )   Objective: To explore the inhibitory effect of berbamine on the proliferation of UMR-106 cells and its possible mechanism in vitro. Objective: To explore the inhibitory effect of berbamine on the proliferation of UMR-106 cells and its possible mechanism in vitro. Methods: UMR-106 cells were cultured with different concentrations of berbamine （0,2,4,8,16 and 32 mg/L） for 24, 48 and 72 h ,respectively,and the proliferation were colorimetrically assayed by MTT. UMR-106 cells were interfered with different concentrations of berbamine （0,4,8 and 16 mg/L）for 24h,the morphological changes of UMR-106 cells were observed by laser confoca1 scan microscope after Hoechst33258 staining, the apoptotic rates were detected by flow cytometry （FCM）using Annexin V/PI staining, the cell cycle were detected by FCM using PI staining. Results: Berbamine could obviously inhibit the proliferation of UMR-106 cells in a dose-dependent manner （P < 0.01）.The IC50 value at 24,48 and 72h were 24.69、8.03 and 3.54 mg/L, respectively. There were nuclear condensation and apoptotic body in berbamine treated group. Apoptotic rates of control group and berbamine treated group were （1.64±0.29）% ，（3.58±0.31） %，（6.27±0.47） % and （11.27±1.09） %，respectively.There was a significant difference in apoptotic rate compared with the control group （P < 0.01）. Meanwhile, berbamine can induce cell necrosis .In addition, berbamine significantly increased the percentages of cells in G0/G1 phase and decreased in S phase and G2/M phase compared with the control group （P <0.01）. Conclusion:berbamine can inhibit the proliferation of UMR-106 cells in vitro, inducing cell apoptosis , necrosis and G0/G1 phase arrest may be the mechanism . Related Articles | Metrics

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The Explore Of Relation Between Renal Sympathetic Denervation for Hypertensive Dogs and The Endothelial function. 2012, (10):  1037-1039 .  Abstract ( 833 )   PDF (170KB) ( 2267 )   【ABSTRACT】Objective The aim of this study was to explore the treatment of renal sympathetic denervation for hypertension and discuss the relation between hypertension and sympathetic hyperactivity and endothelial function at the same time. Methods Eightheen mongrel dogs were studied as intervention (n=10) and control (n=8) groups. The intervention group was made the neurogenic hypertension model. Two groups received percutaneous catheter-based radiofrequency. Primary endpoints were changes of mean blood pressure (MAP) before and at 2, 4 and 10 weeks after procedure. The changes of endothelin-1 （ET-1）and calcitonin gene related peptide（CGRP）were detected at preoperation、after remolding and after procedure, and at the same time Ang II of intervention groups were also detected at 2, 6 and 10 weeks after procedure. We assessed the data changes effectiveness by repeated measures ANOVA. Results In intervention group, MAP、ET-1、CGR had changed significantly(P<0.01) after remolding, compared with baseline level, and MAP、 the level of ET-1、CGRP and AngII all changed significantly after catheter-based renal denervation (P<0.05) . Conclusion Sympathetic hyperactivity plays an important role in the progression of hypertension, and endothelial dysfunction happened in the development of hypertension. Renal sympathetic denervation causes substantial and sustained blood-pressure reduction, with Sympathetic hyperactivity reduced and the improvement of endothelial dysfunction at some degree. Related Articles | Metrics

短篇论著

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Report three cases of Resistance to thyroid hormone(RTH)and analysis thyroid hormone receptorβ gene 2012, (10):  1040-1042 .  Abstract ( 1034 )   PDF (204KB) ( 2246 )   Aims We report the clinical characteristics and the genetic analysis of their family members of three cases. Resistance to thyroid hormone (RTH), a dominant inherited syndrome and it is usually due to mutations located at the ligand-binding domain and adjacent hinge region of the thyroid hormone receptor β (TRβ). Methods: We describe patients’ clinical features, biochemical and hormones level such as thyroid function tests (TFTs), imagination data . We also detected the TFTs of his family members as well. Direct DNA sequencing of the TRβ gene was done for all those with abnormal TFTs. Results: The RTH children had goiter, irritability, aggressiveness, and hyperhidrosis. The TFTs showed high levels of circulating free thyroid hormones (FT4 and FT3) and normal or high thyroid-stimulating hormone (TSH) concentrations. All of these patients used bromocriptine, and then clinical presentations got improved obviously without obvious advert effect. From these three patients and their family members we identified a novel missense mutation, A317D, located in exon 9 of the gene of a boy patient and his mother, but his mother showed no any clinical presentation. However, we didn't have any abnormal finding in for other patients and their parents.Conclusion: The RTH children had goiter, irritability, aggressiveness, and hyperhidrosis. The TFTs showed high levels of circulating free thyroid hormones (FT4 and FT3) and normal or high thyroid-stimulating hormone (TSH) concentrations. Fund a novel mutation in the TRβ in one patient and his mother. This research verified the phenomena that there is a heterogeneous within the same mutation of RTH patients. All of these patients used bromocriptine, and then clinical presentations got improved obviously without obvious advert effect. Related Articles | Metrics

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Effect of homocysteine on the expression of matrix metalloproteinase in human vascular endothelial cell 2012, (10):  1043-1045 .  Abstract ( 797 )   PDF (181KB) ( 2274 )   Abstract Objective: To investigate the mechanism of artherosclerosis by disfunction of vascular endothelial cell with hyperhomocysteinaemia , we observe the effects of homocysteine on the genetic expression of matrix metalloproteinase-1 in human vascular endothelial cell. Method: Incubate HUVEC CRL-1730(human umbilical vein vascular endothelia cell) in vitro with difference concentration (0、10、50、100、1000μmol/L) of homocysteine, then collecte cells in 1,6 and 24 hours after incubation. To semiquantitative the mRNA expression of MMP-1by RT-PCR(reverse transcription- polymerase chain reaction) method. Result: Homocysteine of pathology concentration (50-1000μmol/L) increased the expression of MMP-1 significantly in a dose-depended manner(p<0.05). Conclusion: Pathology concentration homocysteine change the expression of MMP-1 in vascular endothelial cell and up-regulated the expression of MMP-1 a dose-depended manner. So homocysteine effects on the genetic expression of matrix metalloproteinase-1 in human vascular endothelial cell and then promoted extracellular matrix degradation,induced artherosclerosis and plaque disruptive . Related Articles | Metrics

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Transient ischemic attack is neuroprotective for the secondary ischemic cerebral infarction 2012, (10):  1045-1046 .  Abstract ( 807 )   PDF (140KB) ( 2253 )   Abstract Objective: To confirm whether transient ischemic attack(TIA) is neuroprotective for secondary ischemic cerebral infarction. Methods: 60 patients suffered from ischemic cerebral infarction were recruited. 30 of them were enrolled in the TIA group, who all experienced 2~3 times TIA in one week before the cerebral infarction attacks and the TIA always persisted for 5～30 minutes. The other 30 without previous confirmed TIA history were recruited into the control group. CSS was applied to evaluate the degree of neurologic impairment when the patients were admitted. Calculation of the infarction volume was performed 72 hours later after admission. The one month prognosis after admission was assayed using ADL. Results: In TIA group, 22 (73.3%) and 8(26.7%) patients showed mild and moderate neurological impairment respectively. In the control group, the percent of the patients suffering from the mild and moderate neurological impairment was 46.7% (14 cases) and 53.3%(16 cases) respectively. When compared to the control group, more patients in the TIA one showed mild neurological impairment and less one suffered from moderate neurological injury (p<0.05). The mean cerebral infarction volume was significant less in the TIA group with (6.81±2.46) cm3 than in the control one with (10.39±4.53)cm3（p<0.05）. One month later, 24 patients (80.0%) in TIA group recovered to ADL grade I, while only 16 patients (53.3%) in the control one had the grade I outcome (p<0.05) . Conclusion: TIA can alleviate ischemic neurologic injury, reduce the infarction volume and then improve the prognosis. The reason might be TIA induces ischemic tolerance effect on brains and lead to neuroprotective effect on the subsequent cerebral infarction. Related Articles | Metrics

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Analyzing to sixty-two hemodialysis patients with chronic central venous catheter in left jugular vein in early stage for hemodialyzing. 2012, (10):  1047-1049 .  Abstract ( 850 )   PDF (428KB) ( 2348 )   Objective To observe the blood volume in early stage for hemodialyzing with chronic central venous catheter in left jugular vein, analyze the reasons to the catheter dysfunction. Methods Sixty-two end stage renal disease patients were recruited. The places where the catheters were inserted were in left internal jugular veins. The subjects were classified into two groups according to the catheter function. X ray in chest were taken to determine the catheter tip position. Their ages, body height, hemoglobin, platelets, activated partial thromboplastin time and albumin were collected. Relevance between blood volume in hemodialysis and other parameters were analyzed too. Results The volume of blood flow in dialyzing were 261.5±21.6ml/min and 235.2±44.5ml/min separately (P=0.003). And the dysfunction rates between tip position located in atrium and other positions weren’t different (P=0.263). Relevance were found between blood volume and activated partial thromboplastin time(r=0.302, P=0.017). Conclusion. The rate of catheter dysfunction is higher in patients with chronic catheter in left jugular vein. Adjust the position of the catheter when the position of the catheter isn’t in the proper location. The incidence of catheter dysfunction will be reduced if some drugs such as warfarin were taken. Related Articles | Metrics

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Clinical Observation of the MMC on One-site Phacotrabeculectomy 2012, (10):  1049-1051 .  Abstract ( 814 )   PDF (180KB) ( 2342 )   Objective：To evaluate the early effects of mitomycin C（MMC）on combined phacoemulsification, posterior chamber intraocular lens implantation, and trabeculectomy (phacotrabeculectomy) in patients with primary angle closure glaucoma (PACG).Methods：Thirty-three(thirty-three eyes) patients who were dignosed with PACG combined with catract were randomly divided into two groups ,MMC group(fourteen eyes) and control group(nineteen eyes).All the cases were treated with phacotrabeculectomy.Visual acuity, intraocular pressure (IOP), and complications and bleb survival probability were evaluated.Results：The best corrected visual acuity（BCVA）improved and mean IOP decreased significantly after phacotrabeculectomy in both groups. The mean IOP reduction was significantly greater in eyes with MMC group in 12w.(P <0.05). The bleb survival probability in MMC group was higher than control group,but was not significant (P>0.05).The early postoperative complication rates were similar in both groups.Conclusion：Phacotrabeculectomy with MMC results in greater IOP reduction which can be effective method in controlling intraocular pressure level. Related Articles | Metrics

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The preventive effects of intensive doses of atorvastatin on contrast induced nephropathy in patients undergoing percutaneous coronary intervention 2012, (10):  1053-1055 .  Abstract ( 718 )   PDF (173KB) ( 2210 )   Objective: To investigate the preventive effects of intensive doses of atorvastatin on contrast induced nephropathy（CIN） in patients undergoing percutaneous coronary intervention（PCI）.Methods: Two hundred and forty-two patients who are about to undergo PCI in Tianjin Chest Hospital are randomly divided into eithe conventional treatment group（n=120） or intensive treatment group（n=122）.Patients in the two groups are given atorvastatin 20mg and 40mg respectively dialy two days before PCI. The levels of blood urea nitrogen（BUN）, serum creatinine（Scr）, creatinine clearance rate （Ccr）, estimated glomerular filtration rate （eGFR）, interleukin-6（IL-6）, β2-microglobulin（β2-MG）, C-reactive protein（CRP）, tumor necrosis factor-α（TNF-α）, alanine aminotransferase（ALT）were measured at admission and 72h after the procedure. Result:（1）In the intensive treatment group, only 3 patients present with CIN，the incidence of CIN was 2.5%;In the conventional treatment group, CIN occur in seven patients, the incidence of CIN was 5.8%（χ2=1.74，p>0.05）.（2）There was no statistically difference in the levels of BUN、Scr、IL-6 、β2-MG、CRP、TNF-α at admission（P>0.05）. 72h after the procedure, the levels of IL-6, β2-MG, CRP, TNF-α were lower in intensive treatment group than the conventional treatment group, and the levels of Ccr and eGFR were higher in intensive treatment group. Conclusion: Although there was no statistically difference in the incidence of CIN between the 2 groups , the statistically differences in biochemical parameters indicate the tendency of renal protection of intensive treatment before PCI. The protection mechanism may be due to the anti-inflammatory effects. Therefore, the adminstintion of intensive atrovtatin before PCI can be beneficial in the prevention of CIN. Related Articles | Metrics

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Clinical study of serum bilirubin and uric acid in patients with cerebral infarction 2012, (10):  1056-1057 .  Abstract ( 882 )   PDF (142KB) ( 2502 )   Objective: To investigate the role they play in occurring and developing of cerebral infarction, this study focuses on the concentration of serum bilirubin and uric acid in patients with cerebral infarction. Method: All the 233 patients with cerebral infarction were taken fasting blood sample to detect serum total bilirubin, direct bilirubin, indirect bilirubin, uric acid. Control group includes 187 healthy employees of our hospital. Result: The concentration of serum total bilirubin and direct bilirubin in patients with cerebral infarction is statistically higher versus controls（P=0.031，0.000）. Moderate and sever group has higher serum bilirubin concentration compare with mild group（P<0.05）. A statistically significant rise in serum bilirubin was found in patients with acute cerebral infarction compare with sub-acute group and controls（P<0.05）, but the sub-acute group doesn’t have significant difference with controls. The concentration of serum uric acid in patients with cerebral infarction is statistically higher versus controls（P=0.007）. Moderate and sever group has lower serum uric acid concentration compare with mild group（P=0.021）. A statistically significant rise in serum uric acid was found in patients with acute and sub-acute cerebral infarction patients compare with controls（P<0.05）, but no significant difference was found between acute group and sub-acute group. Conclusion: Serum bilirubin, as an anti-oxidant, elevates during acute period and decreases during sub-acute period. The more severe the neurological dysfunction is, the higher serum bilirubin appears. The increase of serum uric acid is not just at acute period after cerebral infarction. After acute period, the serum uric acid is still high due to atherosclerosis. The moderate and severe group has lower serum uric acid maybe because consumption of uric acid as anti-oxidant. Related Articles | Metrics

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Clinical effects of Lamotrigine monotherapy among children patients with newly diagnosed epilepsy 2012, (10):  1058-1059 .  Abstract ( 821 )   PDF (173KB) ( 2323 )   Objective: To understand the clinical effective of lamotrigine for Children patients with newly diagnosed epilepsy. Methods: 80 cases of children patients with newly diagnosed epilepsy, were treated with lamotrigine. LTG was used originally at a dose of 0.5mg/(kg?d), 4 weeks achieved the goal dosage (4.0-5.0mg/(kg?d)). We observed their changes of seizure frequencies compared with the basic-line before the treatments. Result: There are 31 patients seizure free, 27 patients whose frequencies were reduced by more than 75%, 8 patients’ frequencies were reduced by more than 50%. The total effective reached 82.5％. Patients with GTCS obtained the best effects. The total effective reached 95.0％. Related adverse effcts included asthenia, somnolence, skin rashes. These adverse effects disappeared spontaneously without any special treatment. Conclusion: lamotrigine is a safe effective drug for Children patients with newly diagnosed epilepsy. Related Articles | Metrics

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The investigation and study of anemia during pregnancy among nationwide station hospital from 2001 to 2011 2012, (10):  1060-1061 .  Abstract ( 877 )   PDF (145KB) ( 2341 )   Objective:To understand the annual nationwide station hospital patients with gestational anemia prevalence rate and related factors from 2001 to 2011. Methods: A retrospective analysis of the clinical data of anemia during pregnancy for the patients who were treated from October 2000 to September 2011 collected by the Chinese people's Liberation Army General Logistics Department information and technology center case inquiry system ,there were 26339 cases in total and 7986 of them were diagnosed as anemia during pregnancy. The illness circumstances analysis of different age structure, stage of pregnancy and anaemia type, examination of its related factors. Results: The prevalence rate of mild, moderate and severe anemia in gestational period was 56.17%, 43.7 and 0.13%, respectively. The incidence of anemia in the 31-40 age groups was higher than 21-25 and 26-30 age groups. The former group was moderate anemia in majority and the other two groups was mainly mild anemia. The prevalence rate of anemia during pregnancy was increased during 2001-2006, maintained at high levels during 2006-2009, and decreased after 2009. The incidence of anemia was higher in third trimester than that in first and second trimester; moderate anemia was in majority in first and second trimester, while there is mainly mild anemia in third trimester. The anemia during all stage was mostly iron deficiency anemia. Conclusion: We should strengthen the knowledge and skill training for obstetrician in pregnancy nutrition and diet guidance,while enhance the high-risk pregnant perinatal care, reasonablely adjust dietary structure, routinely complement multivitamins and trace elements from the beginning of the second trimester, especially iron, to prevent and reduce the occurrence of anemia. Related Articles | Metrics

临床论丛

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Comparison of laparoendoscopic single-site surgery and retroperitoneal renal cyst decortication YU LI2, 2012, (10):  1067-1068 .  Abstract ( 798 )   PDF (189KB) ( 2309 )   Objective: To investigate efficacy，safety，and feasibility of the two laparoscopic renal cyst decortications. Methods: From August 2009 to June 2011，the clinical data of 127 cases received laparoscopic renal cyst decortication were analyzed retrospectively．Results: Except for one case converting into open surgery,all operations were successfully performed. Conclusion: LESS group had a bit longer operative time. There were advantages in the hospital days and complications, etc. Related Articles | Metrics

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The 20 cases of the treatment of severe superficial femoral artery lesion using Silverhawk plaque biopsy system xiu-jun ZHANG 2012, (10):  1070-1071 .  Abstract ( 814 )   PDF (172KB) ( 2474 )   Abstract: Analysis of hospital clinical data of 20 cases of severe lower limb arterial occlusion in the period from November 2010 to September 2011，17 cases were male and 3 females, age 56 to 67 years, the average age of 60.2 years，Are good results after treatment of the Silverhawk plaque biopsy system，17 patients with Simple plaques peeling treatment success，2 patients with plaques peeling joint balloon angioplasty.1 patient found that the vascular injury, and improved after treatment. 19 patients with plaques peeling treatment success，One cases of failure， Success rate of 95%，20 cases of postoperative symptoms improved，Lesion segment vascular CTA、ankle-brachial index prompted ischemia improved significantly after Atherectomy，Confirmed the safety and efficacy of the Silverhawk plaques biopsy system is an effective method for treatment of severe lower limb arterial lesions Related Articles | Metrics

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Clinical research of double mirror minimally invasive operation and laparotomy in treatment of cholecystolithiasis and choledocholithiasis Ming CHEN 2012, (10):  1072-1073 .  Abstract ( 814 )   PDF (158KB) ( 2597 )   Abstract: 【Objective】 To investigate clinical curative effect on endoscopic sphincterotomy ( EST ) combined with laparoscopic cholecystectomy ( LC ) and laparotomy in treatment of cholecystolithiasis and choledocholithiasis.【Method】60 cases with cholecystolithiasis and choledocholithiasis collected were analyzed,compared the effect of double mirror minimally invasive operation ( 30 cases) and laparotomy (30 cases ).【Results】Double mirror minimally invasive operation was significantly better than laparotomy in bleeding,hospitalization time, residual stone rate and infection of incisional wound ( P < 0.05 ), The difference was statistically significant in iatrogenic pancreatitis rate(23.3%)and bile leakage rate(13.3%) .【Conclusion】There are less trauma, less bleeding, shorter hospitalization time, no residual stones, rapid postoperative recovery in double mirror minimally invasive treatment of gallstones and bile duct stones, still some complications. Related Articles | Metrics

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Acute myocardial infarction complicated with thrombosis of mesenteric artery 2012, (10):  1076-1077 .  Abstract ( 837 )   PDF (179KB) ( 2441 )   Related Articles | Metrics

综述与讲座

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tissue factor and thrombosis Men Jianlong ,ren jing 2012, (10):  1078-1081 .  Abstract ( 874 )   PDF (207KB) ( 2226 )   Related Articles | Metrics