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15 August 2012, Volume 40 Issue 8 Previous Issue    Next Issue
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短篇论著
临床论丛
论著
实验研究
短篇论著
药物临床观察
临床论丛
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Clinical value of electrochemiluminescence immunoassay for measuring Thyrotropin receptor antibody 2012, 40 (8):  0-0 .  Abstract ( 1547 )   PDF (432KB) ( 2734 )   Abstract 0bjective: We evaluated clinical value of fully automated electrochemiluminescence immunoassay (ECLIA) for measuring serum TRAb. Methods: Totally 150 Graves’disease（GD）, 56 Hashimoto’s thyroiditis（HT）,73 subacute thyroiditis(ST) and 95 healthy subjects(HS) were retrospectively evaluated by both ECILA and radioactive receptor assay(RRA). The optimal cut-off which was calculated by receiver operating characteristic (ROC).Results: The cut-off value of 1.85 IU/L from the ROC on the GD cases was chosen as the cut-off with 94.7% of sensitivity and 91.6% of specificity. The area under the ROC curve was 0.935. The new automated assay was superior to RRA. Conclusion: Results demonstrate that this fully automated system has a high sensitivity and speci?city for diagnosis GD, especially for identifying it from various forms of thyrotoxicosis.. This assay may represent the future technology for TRAb detection. Related Articles | Metrics

临床论丛

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Pathogenic study of atypical pathogens infection in hospitalized patients with community acquired pneumonia 2012, 40 (8):  0-0 .  Abstract ( 580 )   PDF (385KB) ( 1932 )   Abstract Objective: to analyse virus, mycoplasma, chlamydia and legionella infection status of community acquired pneumonia (CAP) in hospitalized patients and to provide reference resources for empirical treatment of CAP. Methods: 1079 with CAP who were treated in the respiratory medicine department of Hebei unitedu niversity affiliated hospital during October 2001 to June 2004 and January 2008 to December 2010 were objects of study. All patients’ serums were collected for IgM antibody detection (including chlamydia, mycoplasma, Legionella and virus such as EB virus, adenovirus, respiratory syncytial virus, cytomegalovirus, herpes simplex virus, Coxsackie virus, rubella virus) in the day be hospitalized and 7-10 days later at the same time data in different period were analysed. Results：In 1079 patients, positive results include 165(15.29%) chlamydia, 99 (9.18%) mycoplasma, 58(5.38%) legionella and 358(33.18%) virus. In comparison, the detection rates of legionella, syncytial virus, cytomegalovirus, herpes simplex virus, rubella virus and EB virus were decreased obviously. Antibodie’s detection rate of Coxsackie, EB and syncytial virus in the onset of 10~20 days was higher than that in 9 days ago and 20 days after. Conclusion：Atypical pathogens are important pathogenic in CAP, mycoplasma infection is one of the most common reasons. In comparison, infection rate of atypical pathogens in patients with CAP was not significantly increased and in part has a downward trend. In the onset of 20 days, most positive result of IgM antibody can be found in patient with.atypical pathogens infection. Related Articles | Metrics

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To Investigate the Genetic Polymorphisms of STR Loci D21S11, D21S1440 and Penta D in Tianjin Han Population 2012, 40 (8):  753-756 .  Abstract ( 860 )   PDF (471KB) ( 2460 )   Objective: To investigate the genetic polymorphisms of 3 short tandem repeat (STR) loci D21S11，D21S1440 and Penta D on chromosome 21 in Tianjin Han population. The aim of the study was to provide basic data for the use of 3 STR loci in gene diagnosis and prenatal gene diagnosis of Down syndrome (DS). Methods: Blood samples from 332 unrelated individuals of Tianjin Han population were collected. QF-PCR and ABI PRISM 377 sequence were used. The frequencies of the genotypes were tested with Hardy-Weinberg equilibrium. Genetic analysis was performed to conclude some data of population genetics such as the frequency of the alleles，the heterozygosity of observation(Ho)，the polymorphism information content(PIC)，the probability of discrimination power(DP)，and the probability of exclusion(PE). Results: 6，4，8 alleles of D21S11，D21S1440 and Penta D were observed respectively. The frequencies of the genotypes were in good agreement with Hardy-Weinberg equilibrium. The Ho of 3 STR loci were 0.617, 0.705, 0.867. The PIC were 0.755, 0.596, 0.795. The DP were 0.916, 0.794, 0.931. The PE were 0.312, 0.436, 0.730. Conclusion: D21S11，D21S1440 and Penta D STR loci had high heterozygosity in Tianjin Han population. They were good genetic markers of chromosome 21 and can be used in gene diagnosis and prenatal gene diagnosis of DS. Related Articles | Metrics

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Expression and significance of EMP1 in oral squamous cell cancer tissues 2012, 40 (8):  757-759 .  Abstract ( 776 )   PDF (1333KB) ( 2450 )   Objective: To investigate Expression and significance of EMP1 in oral squamous cell cancer tissues. Methods: Immunohistochemical method was used to determined expression of EMP1 in 60 of oral squamous cell cancer (OSCC) tissues. Statistical analysis was performed for the correlation between expressive intensity of EMP1 and availably clinic parameters, the difference between early and advanced tumors and between lymph node and no lymph node metastasis.Sensitivity and specifity of expressive scores of EMP1 used for diagnosis were identified. Results: Expressive intensity of EMP1 correlated with clinic stage (P=0.002) and lymph node metastasis or not (P=0.044). There was a significant difference existed between early and advanced stage, lymph node and no lymph node metastasis (P=0.003, and P=0.045, respectively). Sensitivity and specifity of expressive scores of EMP1 used for diagnosis of lymph node metastasis and early and advanced OSCC were 61.90%, 77.80% and 70%, respectively. Conclusion: Expression of EMP1 was correlated with lymph node metastasis or not and clinic stage, which may has reference significance for clinical practice. Related Articles | Metrics

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A clinical research on combination of mild hypothermia and hyperbaric oxygen following by severe traumatic brain injury 2012, 40 (8):  760-762 .  Abstract ( 778 )   PDF (445KB) ( 2471 )   【Abstract】Objective To assess the clinical effects of mild hypothermia and hyperbaric oxygen injection following by severe traumatic brain injury. Methods According all 157 patients with acute severe traumatic brain injury were divided into mild hypothermia group (54 patients), hyperbaric oxygen with mild hypothermia group (48 patients) and routine treatment group (55 patients) randomly. The therapeutic effect was evaluated at the 3rd month after injury by Glascow Outcome Scale (GOSs). One hundred and one patients (vegetable alive and dead patients were not included) were evaluated at the 3rd month after injury by Barthel Index. Result In comparison with other two groups, there was a higher mortality rate in routine treatment group (P<0.05). There were significant difference for recovery rate in three groups (P<0.05). Conclusions Mild hypothermia was an effective way to severe traumatic brain injury, which could reduce the mortality rate and improve the prognosis without severe complications. The hyperbaric oxygen injection had some effect for recovery. Related Articles | Metrics

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Study of antituberculosis action of baicalin on Mycobacterium tuberculosis 2012, 40 (8):  763-765 .  Abstract ( 963 )   PDF (563KB) ( 2626 )   object: To analyze the antituberculosis action of baicalin on Mycobacterium tuberculosis by Antimicrobial Susceptibility Testing in vitro. methods: Detecting Bacteriostatic Action of baicalin on Mycobacterium tuberculosis by absolute consistence method of Lowenstein-Jensen. results: MIC minimum of baicalin on 37 strains Mycobacterium tuberculosis was 1.5 g/L, MIC maximum was ＞48 g/L.MIC90 was 12 g/L, MIC75 and MIC50 was 6g/L.The number of drug sensitive Mycobacterium tuberculosis was most in 6 g/L MIC, and decrease progressively with MIC value reduce or increase. there was not relevance between the strain number of INH mild drug resistance Mycobacterium tuberculosis and the MIC value. The number of EMB mild resistance strain was increase progressively with MIC value increase（MIC 3-9g /L）. The number of RFP mild resistance strain was decrease progressively with MIC reduce（MIC 2-8 g/L）.conclusion: there is antituberculosis action of baicalin on Mycobacterium tuberculosis. Related Articles | Metrics

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Experimental Studies on processing and Property of Scaffold Materials of Simvastatin loaded Bio-Derived Bone 2012, 40 (8):  766-768 .  Abstract ( 843 )   PDF (3503KB) ( 2386 )   Abstract Objective: Simvastatin loaded collagen tissue engineering bone scaffold materials were built, to detect the physical and chemical properties, mechanical strength of these materials, and provide the choosed scaffold materials for experiment and clinical of tissue engineering. Methods: Pure tissue engineering bone scaffold materials, collagen loaded tissue engineering bone scaffold materials, simvastatin loaded tissue engineering bone scaffold materials were made by using allograft, and type-I collagen, and simvastatin. Their morphological features, constitute components and mechanical properties were examined by scanning electron microscopy, x-rays diffraction, mechanical assay. Results: Pure tissue engineering bone scaffold materials maintained natural network pore system; Collagen loaded tissue engineering bone scaffold materials maintained natural network pore system, the surface of network pore system was coated by collagen membrane; simvastatin loaded collagen tissue engineering bone scaffold materials maintained natural network pore system, the surface of network pore system was coated by collagen membrane. Pure tissue engineering bone materials, collagen loaded tissue engineering bone materials, Simvastatin loaded tissue engineering bone scaffold materials were consisted of [HA,Ca10 (OH)2(PO4) 6]. There was no significant stastical difference among the compression strength, bending strengthof the three scaffolds materials. Conclusions: Simvastatin loaded tissue engineering bone scaffold materials is similar with the other scaffold materials in physical and chemical properties, mechanical strength , and it is a kind of effective scaffold materials for tissue engineering bone. Related Articles | Metrics

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The serum concentrations of RBP-4 and IL-6 in patients with Graves disease and Type 2 diabetes Qi-Hang MA, 2012, 40 (8):  769-771 .  Abstract ( 1089 )   PDF (378KB) ( 2504 )   ? Abstract Objective: To investigate the serum concentrations of RBP-4 and IL-6 related to insulin resistance in patients with Graves disease and type 2 diabetes.Methods: One hundred and seven patients with Graves diease were divided into initial diagnosis group(n=54) and treatment group (n=53) . Type 2 diabetes group(n=53).Fifty healthy subjects were selected for the control. The levels of glucose，insulin，free triiodothyronine ，free thyroxine，thyrotropin，antithyroglobulin antibody ，anti-thyroperoxidase antibody and anti-thyrotropin receptor were detected using fasting blood. The serum levels of RBP-4 and IL-6 were detected by enzyme-linked immunosorbent assay (ELISA).Result:The serum levels of RBP-4 in initial diagnosis group, treatment group and type 2 diabetes group were higher than control group(P＜0.001、P＜0.001、P＜0.001). The serum levels of IL-6 in initial diagnosis group were higher than the treatment group ,type 2 diabetes group and control group (P＜0.001、P＜0.001、P＜0.001). Conclusion:Insulin resistance in Graves disease patient may relate to high serum level of RBP-4 or IL-6. Related Articles | Metrics

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Association between serum vaspin concentrations and insulin sensitivity, glucose and lipid metabolism in the obese children 2012, 40 (8):  772-775 .  Abstract ( 1140 )   PDF (466KB) ( 2438 )   Objective: To investigate the relationship between serum vaspin concentrations and insulin sensitivity, glucose and lipid metabolism in the obese children. Methods: A total of 34 obese children (22 males and 12 females) were enrolled in this trial. And another randomly selected 30 cases of normal healthy children were taken as control. Fasting venous blood samples were collected for the determination of the serum vaspin level by Enzyme-linked immunosorbent assay (ELISA). The Physical index such as anthropometric weight, height and the FPG, FINS were measured using standard instruments and procedures, and the body mass index (BMI), Waist-hips-ratio (WHR), body fat % (BF%), HOMA-IR, ISI and AI were calculated according to suitable formula. the relationship between serum vaspin concentrations and insulin sensitivity, glucose and lipid metabolism was analyzed. Results: The vaspin, WC, BMI, BF%, SBP, DBP, FPG, FINS, LDL-C, TG, LDL-C/HDL-C, (TC-HDL-C)/HDL-C, HOMA-IR of the obese group were much higher, while the HDL-C, ISI were much lower than the control group. There is a significant positive correlation between the vaspin concentrations and BMI, WC, WHR, BF%, TG, FINS, HOMA-IR, (TC-HDL-C)/HDL-C, DBP, While a negatively correlated with HDL-C, ISI. Multiple linear regression suggests that BF% is an independent risk factor for the serum vaspin concentrations. Conclusion: The serum vaspin concentration was strongly associated with obesity and atherogenic index. Monitoring the serum vaspin levels may have a very important value for understanding the degree of obesity and insulin resistance. Related Articles | Metrics

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Comparison of circumferential pulmonary vein isolation and antiarrhythmic drug therapy for atrial fibrillation Hui-Yong WANG 2012, 40 (8):  780-783 .  Abstract ( 770 )   PDF (454KB) ( 2599 )   Comparison of circumferential pulmonary vein isolation and antiarrhythmic drug therapy for atrial fibrillation WANG Hui-yong；LI guang-ping；XU Gang；CHEN Xin；YE Lan；XU Yan-min Cardiology Department，Second Hospital of Tian jin Medical University ,Tian jin 300211, China Abstract Objective: to determine the clinical efficacy of Circumferential pulmonary vein isolation(CPVI) compared with antiarrhythmic drug therapy (ADT) on sinus rhythm (SR) maintenance in patients with atrial fibrillation (AF). Methods: 402 patients with atrial fibrillation were divided two groups，CPVI group(n=143) and ADT group(n=259). By clinic service ,telephone and letter, the patients after discharged were followed up .The primary end point was AF recurrence.The survival analysis was performed the Kaplan-Meier analysis and log-rank test. Results: During following-up 82 patients in the CPVI group maintained sinus rhythm(SR),while 63 patients in the ADT group were free from recurrent AF.By log-rank test and Kaplan-Meier analysis, 57.3%of patients in the CPVI group and 24.3% of those in the ADT group were no recurrent AF (P <0.001). The multivariate analysis showed AF duration ,left atial diameter, treatment assignment were independent predictors of AF recurrences.Conclusion: Circumferential pulmonary vein isolation is superior to Antiarrhythmic drugs for preventing AF recurrence in patients with atrial fibrillation． Related Articles | Metrics

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Expression and clinical significance of adhesion molecule L1 in gastrointestinal stromal tumors 2012, 40 (8):  784-786 .  Abstract ( 1190 )   PDF (3625KB) ( 2471 )   Abstract　Objective: To investigate the expression of L1 in gastrointestinal stromal tumors (GIST)and its significance. Methods: L1 Protein and mRNA were detected in the tissues of 66 cases of GISTs , 10 cases of smooth muscle tumors, 6 cases of schwannoma and 14 cases of the normal bowel by immunohistochemistry and RT-PCR. And the follow up was carry out.The correlation of L1 expression with clinical pathologic features of GIST and the difference L1 expression in between GIST and smooth muscle tumors, schwannoma, the normal bowel tissue were analyzed. Results:　The expression rates of L1 Protein and mRNA in GISTs were 57.6%(38/66) and 83.3% (30/36) , and the difference of the both was significant ( P < 0.05). the expression of L1 had significant difference in different aggressive risk of GIST(P<0.05),but had no correlation with age，sex ,locus, tissue type and survival rate in GIST（P>0.05）. L1 expression in GIST was significant higher than it in smooth muscle tumors and the normal bowel tissue ( P < 0.05) and had no significant difference with it in schwannoma. Conclusion: The expression of L1 may play an important role in tumorigenesis and progression of GIST, which suggest that L1 is applied to diagnosis or differential diagnosis of GIST; Future studies might focus on the impact of the expression of L1on survival. Related Articles | Metrics

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Expression and Clinical Significance of WWOX and P53 Genes in Colorectal Carcinoma Tissues 2012, 40 (8):  787-789 .  Abstract ( 942 )   PDF (394KB) ( 2635 )   This study detected the expression of WWOX and P53 in the tissue of colorectal Cancer，to discuss the correlation of WWOX and P53 expression and clinical pathologic parameters，and to guide the early diagnosis or judge rognosis of colorectal Cancer and provide theoretical foundation for prevention and clinical treatment．Methods: The tissues from 45 cases of colorectal Cancer and 25 Cases of normal tissues were evaluated for the expression of WWOX and P53 by Immunohistochemical staining methods，the levels of these proteins in tissues with different types and their correlation with colorectal Cancer were analyzed．And the biological behavior of colorectal Cancer Were analyzed，including patients’sex，age，size，differentiation degree，infiltration depth and lymph nodes metastasis．Results: There was a significantly much lower positive rate of WWOX proteins in colorectal Cancer than that in normal tissues group(P<0.05)，While There was a significantly much higher positive rate of P53 proteins in colorectal Cancer than that in normal tissues group(P<0.05), The positive rates of WWOX and P53 expression was closely correlated with histological grade and infiltration depth and lymph node metastasis．There was not obvious relation with the patient，s age and gender(P>0.05). the expression of WWOX and P53 gene demonstrated significantly negative relation(r=-0.388，P=0.008)．Conclusion: WWOX and P53(wild type) gene are all tumor suppressor gene，and they may have synergistic action in the generating，developing and transferring process of colorectal cancer． The joint investigation of expression has more important clinical significance． Related Articles | Metrics

实验研究

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The Effects of Diaomozhibeng liquid Uterine Cavity Target Intervention on Uteri Endometrial tissue of ADUB in Model Rats on the Expression of ER、 ERα、ERβ Feng-Jie HE 2 2012, 40 (8):  793-795 .  Abstract ( 756 )   PDF (2850KB) ( 2380 )   Objective: To explore the role and the possible mechanism of Diaomo Zhibeng liquid on the rats with the endometrial hyperplasia. Methods: The model of endometrial hyperplasia was induced with estrogen load after the induction of model in rats with EH. The medicine of Diaomo Zhibeng liquid were administrated in uterine cavity of rats.The expression levels of ER、ERα、β were checked by immunohistochemistry. Results: Compared with control group, the expression of ER、ERα、β increased in the rats of the model group (P<0.05). However, after treatment of Diaomo Zhibeng liquid, the expression of ER、ERα、β decreased, (P<0.05). There is no difference between small and big dose of Diaomo zhibeng liquid groups (P>0.05). Conclusion: Diaomo Zhibeng liquid plays a role in the endometrial hyperplasia by accelerate the ecclasis of uterine endometrial. One of mechanisms is maybe related to inhibiting the expression of ER、ERα、β. Related Articles | Metrics

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The changes of histomorphology of dorsal root ganglion and tissues around after implanting the radioactivity of 125I seeds around the interverterbal foraman of lumbar. 2012, 40 (8):  800-802 .  Abstract ( 695 )   PDF (1549KB) ( 2362 )   【Abstract】Objective: To observe the effects of different radioactivity of 125I seeds on the histomorphology of dorsal root ganglion and tissues around by implanting radioactive seeds 125I around dorsal root ganglion.Methods: The experiment were carried out using twenty four adult male Sprague-Dawley rats (220-250g), randomly assigned to four groups. the different radioactivity 125I seeds were implanted around dorsal root ganglion ,respectively named 0mCi group (n=6), 0.4mCi group(n=6), 0.6 mCi group(n=6), 0.8 mCi group(n=6). CT scan were used to check the seeds whether locating in 1cm range around dorsal root ganglion.if not in the range ,the rat must be rejected. the two rats were killed at 60 days randomly to observe the histomorphology of L5 dorsal root ganglion and the surrounding tissues. Results: the implantion of 125I seeds was successful around L5 DRG in 24 rats.(2 rats were rejected, then supplement in time), CT scan ensure exact position. The cellular swelling of the 0mCi group was moderate The cell edema was more severe , the nucleus was not in center and Karyopyknosis、fragmentation in the 0.4 mCi group. In the 0.6 mCi group, a large of DRG cell became karyolysis and nuclear condensation. Mass DRG cell revealed karyolysis、nuclear condensation and degeneration. The ratio of positive cells（the nucleus was not in center or Cytoplasmic were 73.80±2.38% in 0.8 mCi group . Conclusion 1.DRG can be injured after implanting radioactive 125I around DRG, mainly characterized by necrosis, the intensity of which were increased as radiological dose increased. Related Articles | Metrics

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The effect of Hepatic Lipase Gene Promoter Polymorphisms on its transcriptional activity 2012, 40 (8):  803-805 .  Abstract ( 715 )   PDF (2327KB) ( 2494 )   Abstract Objective： To construct the recombinant plasmid containing the double luciferase report gene and the HL promoter -514/-250 sites.and tansfect the plasmids to HepG2 cells.Then to analyse HL transcriptional activity. Method: Construct luciferase reporter vector containing HL promoter -514T/-250A and -514C/-250G polymorphisms, tested by Dual Luciferase Reporter Assay System, use Fluoroskan Ascent FL test the 10 second value. Result: The luciferase reporter vector contain with HL promoter -514T+-250A present higher HL transcriptional activity than the vector with -514C+-250G. Conclusion: HL promoter -514C together with -250G alleles are associated with higher HL tanscriptional activity, so proved that HL promoter polymorphisms can influence the HL transcriptional activity on the molecular mechanism. Related Articles | Metrics

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The Effects of Oxidative Stress on Multiple Organ Edema in the Rats suffured from Limb Ischemia-reperfusion and the Precaution of Salvia Miltiorrhiza 2012, 40 (8):  806-808 .  Abstract ( 1154 )   PDF (529KB) ( 2321 )   【ABSTRACT】Objective: To investigate the effects of oxidative stress on multiple organ edema in the rats which suffered from hind limb ischemia-reperfusion(LIR）and the precaution of Salvia miltiorrhiza. Methods: 24 Wistar rats were randomly divided into 3 groups: control group(C), ischemia-reperfusion group(I/R) and Salvia miltiorrhiza group(SM). Referring to routine method in our department, the model rats which underwent 4 hours ischemia and 4 hours reperfusion of hind limbs were made. 30 minutes before reperfusion, SM was given to the rats in SM group by tail vein injection at the dose of 5 ml／kg．Accurately weigh one gram of heart, liver, kidney, lung, brain, intestine and skeletal muscle from every animals，weigh these specimens after baking(60℃,55 hours), Calculated the ratio of wet and dry (W/D) .Measured the activity of SOD and the levels of XOD and MDA in plasma. The morphologic changes of those tissue samples were observed with microscope. Results: It was found that after suffering from ischemia-reperfusion, the W/D of every specimens increased in different degree（P<0.01 or P<0.05）. In plasma, the values of SOD decreased but XOD and MDA increased obviously（P<0.01 or P<0.05）. After LIR, disordered arrangement ,broaden interstitial and infiltration of inflammatory cells of sample can be seen in different degree under microscope. However, Compared with I/R group, all organs’ W/D in SM group are lower, The values of SOD in plasma increased but XOD and MDA in plasma falled down（P<0.01 or P<0.05.Pathological changes in every tissue were reduced. Conclusion: Limb ischemia-reperfusion can lead to multiple organ edema, oxidative stress is one of the important mechanisms. Salvia miltiorrhiza might be play the role of anti-edema by anti-oxidation. Related Articles | Metrics

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Study on Acute Toxicity and Hypoglycemic Activity of Stigmata Maydis Extractive 2012, 40 (8):  809-811 .  Abstract ( 1160 )   PDF (402KB) ( 2677 )   Abstract: Objective: Study on acute toxicity and hypoglycemic activity of stigmata maydis extractive, and observe the hupoglycemic activity. Methods:LD50 and 95%confidence interval by Karber's method. The bloode sugar were determined by normal mice, high glucose mice that be inject glucose or adnephrin. Results: LD50 of stigmata maydis alcoh extract is 203.106(95%confidence interval is 188.665～218.652)g crude drug/kg; LD50 of stigmata maydis aqueous extract is 105.203 (95%confidence interval is 95.249～116.197)g crude drug/kg. There was no influence to normal mice. Stigmata maydis alcoh extract 30g crude drug/kg can lower blood sugar of glucose load mice ; Stigmata maydis alcoh extract and aqueous extract 30g crude drug/kg can lower blood sugar of the mice injected adr (p<0.05，p<0.01). stigmata maydis alcoh extract can add hepatic glycogen (p<0.01). Conclusion: Stigmata maydis alcoh extract and aqueous extract were atotic (LD50＞50g/kg). There was no influence to normal mice. But the two extracts can lower blood sugar of the mice injected adr, and can promote glyconeogenesis and adjust glycometabolism. To compute Related Articles | Metrics

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The chronic effect of palmitate on the function of isolated mouse islets and clonal B cell INS-1E cells 2012, 40 (8):  812-814 .  Abstract ( 916 )   PDF (431KB) ( 2395 )   Objective To explore the chronic effect of excess palmitate on INS-1E cells and isolated mouse islets. Methods Islets were isolated by the collagenase digestion technique from adult NMRI mice. Both passaged INS-1E cells and isolated mouse islets were incubated in RPMI 1640 supplemented with 0 or 0.4 mmol/L palmitate for 72 h, respectively. After preincubation, both INS-1E cells and islets were incubated in Krebs-Ringer buffer containing 3.3 or 16.7 mmol/L glucose for 1 h, respectively. Correspondingly upper medium was taken for insulin assay. Total RNA was extracted from INS-1E cells after 72 h incubation with 0 or 0.4 mmol/L palmitate, cDNA was synthesized, Pdx1, Insulin 1, Insulin 2, and GLUT2 mRNA expressions were tested in INS-1E cells by RT-PCR. Results Basal insulin secretion was increased, and glucose-stimulated insulin secretion was impaired in both INS-1E cells and isolated mouse islets after long-term excess palmitate incubation, and insulin 1, insulin 2, and GLUT2 mRNA levels were diminished in INS-1E cells. Conclusion Long-term exposure to higher level of palmitate concentration may cause dysfunction of pancreatic islet cells. Related Articles | Metrics

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Outcome of different timings in transverse rectus abdominis myocutaneous breast reconstruction after the breast cancer operation 2012, 40 (8):  815-816 .  Abstract ( 828 )   PDF (401KB) ( 2410 )   【Abstract】Objective: To observe the clinical effects of transverse rectus abdominis myocutaneous (TRAM) breast reconstruction after the breast cancer operation and to estimate the timing of TRAM breast reconstruction after the breast cancer operation. Method: In our study, 78 patients with stage Ⅰ or stageⅡ breast cancer were involved. The population was stratified into two groups: group 1, immediate breast reconstruction after mastectomy (n = 46 patients); and group 2, breast reconstruction within 6-12 months after surgery (n = 32 patients). Outcomes of both groups were evaluated including satisfaction, hospital stay, postoperative complications and so on. Result: There was a statistic significance(P＜0.05) between two groups with regard to appearance satisfaction, hospital stay and average cost of treatment; While the postoperative complications, survival of patients after postoperative 2 years and 5 years were similar in both groups。 Conclusion: Our outcomes implied that immediate TRAM breast reconstruction after mastectomy is a reliable and safe technique with high patient satisfaction, short hospital stay, shrinkage of the cost, low complication rates and similar survival rate. Related Articles | Metrics

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Effect of metabolic syndrome on left ventricular function after acute myocardial infarction 2012, 40 (8):  819-821 .  Abstract ( 1108 )   PDF (416KB) ( 2409 )   Objective To investigate the effect of metabolic syndrome on left ventricular function after AMI onset. Methods We prospectively studied the relationship between metabolic syndrome and left ventricular function in 161 patients with AMI. Patients were grouped to metabolic syndrome group(n=86）and non metabolic syndrome group(n=75）. left ventricular end diastolic volume （LVEDV） and LVEF were measured at admission and at 30 days after AMI onset. Results 56.4％of the 161 AMI patients were complicated with MS. Compared with non MS group, LVEDV of MS were increased (for admission: 139.5ml±41.3ml vs.124.4ml±36.9ml，P=0.016; for 30 days: 154.1ml±40.8ml vs.139.1ml±36.3ml，P=0.016)，and There was no difference in LVEF between the 2 groups（for admission: 49.4%±7.2% vs.50.4%±8.3%，P=0.377; for 30 days: 52.5%±6.8% vs.54.3% ±8.3%，P=0.119）. Conclusions There were high prevalence of MS in patients with AMI, and there were not significant effect of MS on left ventricular function after AMI onset. Related Articles | Metrics

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Change of CD4+IL-10+regulatory T Cell in Atopic Dermatitis patients 2012, 40 (8):  821-822 .  Abstract ( 1188 )   PDF (359KB) ( 2529 )   Abstract: Objective To explore the change of CD4 + IL-10 + regulatory T cells and cytokine in atopic dermatitis. Methods The direct immunofluorescence assay was used to detect CD4+ IL-10 +Tcell by flow cytometry. ELISA was used to assay IL-10 and TGF-βin peripheral blood. Results The absolute count of CD4 + IL-10 + T cells is 0.26 + 0.126×109 / L, and the percentage is 12.76 + 4.85% in AD patients . They are higher than in control group(0.016 + 0.007×109 / L; 3.27 + 1.03%). There ere statistically significant (p < 0.05). The peripheral blood IL-10 concentration in AD patients is 126.59+ 59.46 pg/ml, higher than normal control group (31.47 +10.96 pg/ml) (p < 0.05). The peripheral blood TGF-βconcentration in AD patients is 407.23 + 155.88 pg/ml, higher than normal control group (359.47 + 99.21 pg/ml) (p > 0.05). There are statistically positive correlation between absolute and percentage of CD4 + IL-10 + T cell and IL-10 concentration (r = 0.47, r = 0.65, p < 0.05). Whereas There are no statistically correlation between absolute and percentage of CD4 + IL-10 + T cell and TGF-βconcentration (r = 0.15, r = 0.21, p > 0.05). Conclusion IL-10 is the main functional f actor of Trl. It can involved in immune adjustment by cutting inflammation process mediated by Thl and Th2 cell. Related Articles | Metrics

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A study on diagnostic properties of pleural cholesterol to distinguish exudates from transudates ,yue-chuan LI 2012, 40 (8):  823-825 .  Abstract ( 930 )   PDF (514KB) ( 2462 )   Related Articles | Metrics

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A clinical analysis of pathogen epidemiology and risk factors of central venous catheter related bloodstream fungal infection in intensive care unit 2012, 40 (8):  825-827 .  Abstract ( 931 )   PDF (425KB) ( 2613 )   Objective：To investigate the pathogen epidemiology and risk factors of central venous catheter related bloodstream fungal infection (CVC-RBFI) in intensive care unit（ICU）, in order to provide useful information for the prevention and treatment of CVC-RBFI. Methods：The clinical data of 20 cases patients with CVC-RBFI in ICU between July 2006 and December 2010 were retrospectively analyzed, and compared with the control which the clinical data of 35 cases patients with central venous catheter related bloodstream bacteria infection (CVC-RBBI) at the same time. Meanwhile the pathogen epidemiology and drug resistance also were analyzed. Results：CVC-RFBI accounted for 36.36%（20/55）of central venous catheter related bloodstream infection(CVC-RBI). Non-albicans candida accounted for 60%（12/20）of fungal pathogen. The susceptibility rates of non-albicans candida to zole were 50.00%. The independent risk factors of CVC-RBFI were the existing more drainage tubes of body（OR= 8.938，P<0.05）and post major operations（OR= 11.250，P<0.05）. Conclusion： CVC-RFBI is the main party of CVC-RBI in ICU. It would be strengthened to prevent and treat to the risk factors of CVC-RBFI synthetically. Related Articles | Metrics

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Influence of abdominal space in different CO2 pneumoperitoneum pressure in neonates and younger infants undergoing laparoscopy 2012, 40 (8):  827-828 .  Abstract ( 1415 )   PDF (404KB) ( 2486 )   【Abstract】 Objective To observe the change and correlation of abdominal space in different CO2 pneumoperitoneum pressure. Methods Twenty cases undergoing laparoscopic surgery were monitored cubic abdominal circumference, HR, MAP, SiO2, PETCO2 before pneumoperitoneum, pneumoperitoneum pressure 5mmHg and 10mmHg. Result Cubic abdominal circumference, HR, MAP, PETCO2 significantly increased with pneumoperitoneum pressure increasing, P<0.05. Abdominal cavity volume would be increased 35% by pneumoperitoneum pressure 5mmHg, while get another 11% abdominal cavity volume by 10mmHg. Conclusion Since lower pneumoperitoneum pressure could meet requirement of laparoscopic operation, we should pay more attention to the interference of CO2 pneumoperitoneum on physiological function. Related Articles | Metrics

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Papillary thyroid microcarcinoma : a Clinicopathologic analysis of 326 cases , 2012, 40 (8):  829-830 .  Abstract ( 1186 )   PDF (334KB) ( 2602 )   Abstract Objective: To investigate the clinicopathologic characteristics of Papillary thyroid microcarcinoma（PTMC）. Methods: 326 cases of PTMCs in 2001 and 2010 were retrospectively analyzed with the clinicopathologic data. Results: （1）There are 39 PTMCs in 2001, containing 33 unifocal and 6 multifocal lesions; and there are 287 PTMCs in 2010, containing 224 unifocal and 63 multifocal lesions.（2）The incidence of PTMC and multifocal PTMC in 2010 was significantly higher than that in 2001.（3）In 2001 and 2010 receptively, the occuring of lymph node metastasis and capsule infringed in multifocal PTMC is more often than that in unifocal PTMC.（4）Hashimoto’s thyroiditis is frequently presented as background changes in multifocal lesions. Conclusions: The incidence of PTMC and multifocal PTMC increased significantly in past 10 years, and neck lymph node metastasis and capsule infringed is more frequently occurred in the latter one. In conclusion, our studies suggest that multifocal PTMC presented with aggressive behavior, which should be paid more attentions in clinical treatment, and close follow-up are greatly needed. Related Articles | Metrics

药物临床观察

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Effect of different anesthesia on Anti-inflammatory Cytokines in the intracranial neoplasms 2012, 40 (8):  831-832 .  Abstract ( 939 )   PDF (397KB) ( 2793 )   Objective To investigate the influence of anesthesia methods and anesthetics on anti-inflammatory cytokines in the intracranial neoplasms. Methods One hundred six ASA Ⅰ or Ⅱ patients undergoing for selective intracranial neoplasms surgery were divided randomly into two groups with 53 cases each. The patients in group P were given intravenous anesthesia with target controlled infusion of propofol 3~6μg/mL, those in group I received inhalation anesthesia with isoflurane 1% ~2%. IL-4, IL-10 and IL-13 were recorded before anesthesia, 0h, 24h, and 48h after operation. Results The serum levels of IL-4, IL-10 and IL-13 were significantly increased at 24h and 48h after operation compared with those before anesthesia in both groups (all P<0.05). The serum levels of IL-4, IL-10 and IL-13 were significantly higher in group P than those in group I at 24h and 48h after operation (all P<0.05). Conclusion The effect of propofol was better than sevoflurane in inhibiting inflammatory reaction in patients with intracranial neoplasms. Related Articles | Metrics

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Dexmedetomidine infusion for sedation during awake fibreoptic nasal intubation 2012, 40 (8):  833-835 .  Abstract ( 809 )   PDF (439KB) ( 2567 )   【Abstract】 Objective To evaluate the effects of dexmedetomidine infusion on sedation of patients undergoing awake fibreoptic nasal intubation. Methods Thirty ASAⅠ or Ⅱpatients aged 21～72 yr weighing 46～85kg scheduled for elective oral surgery were randomly allocated into 2 groups ( n=15 each): dexmedetomidine group(group D)and fentanyl group(group F). Patients in group D received dexmedetomidine 1μg/kg infusion. Patients in group F received fentanyl 1μg/kg infusion. The fiberoptic nasal intubation time was recorded. The changes of HR, BP, SPO2, BIS, sedation (the Ramsay score), satisfaction in the two group at T1 (before drug administration), T2 (after drug administration) and T3 (intubation) were observed. Results Compared with T1, HR obviously decreased at T2、T3 in group D, while the Ramsay score is increased（P <0.05）. Compared with group F,the change of SBP at T3 is small（P <0.05）. There were no significantly differences of BIS, SPO2 at T1 , T2 , T3 （P >0.05）.The patient’s tolerance and satisfaction scores of group D were better than Group F（P <0.05）. Conclusion Combination of dexmedetomidine loading with topic anaesthesia can allow better sedation, stable haemodynamic status, absence of respiratory depression in awake fibreoptic nasal intubation. Related Articles | Metrics

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Comparison of healing outcomes of incised superficial abscess wounds with honey and traditional dressing 2012, 40 (8):  835-836 .  Abstract ( 810 )   PDF (328KB) ( 2425 )   【Abstract】Objective To compare the healing outcomes of superficial abscess wounds dressed with either crude undiluted honey or traditional gauze. Methods 65 patients with fresh surgical incision and drainage of the superficial abcesses were randomized assigned into honey group（n＝33）and traditional group（n＝32）．The wounds were left to close spontaneously with once every two days wound dressing with either honey or traditional gauze in two randomized treatment groups.Both groups were comparable at baseline. Results After 3 days and 7 days of treatment, wounds treated by honey dressing had less exudation(Points) than those treated by tridational gauze (the third day：t=2.473，P<0.05；the seventh day： t=2.051，P<0.05); Honey appeared to be more effective than traditional dressing in promoting wound healing after 21 days of treatment（Z=2.542，P<0.05；）; Honey appeared to be less painful(Points) than traditional gauze during dressing changes（t=2.443，P<0.05；）; The healing time(days) of the honey group was significantly shorter than the traditional group（t=2.842，P<0.01）; The cure rates were 100% in both groups ; There was no adverse reaction occurred in the two groups. Conclusion The use of honey dressing is effective and practical for incised superficial abcesses. Related Articles | Metrics

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Analysis of hydroxyethyl starch combined with Edaravone to treat progressive stroke li chen 2012, 40 (8):  837-838 .  Abstract ( 744 )   PDF (402KB) ( 2340 )   Object To discuss the treatment of progressive stroke with hydroxyethyl starch combined Edaravone. Method A retrospective analysis of 100 patients with progressive stroke from Jan 2010 to Nov 2011. All of these paitents were randomly divided into two groups, 50 patients in each group. The treatment group was treated with hydroxyethyl starch and Edaravone on the base of routine treatment. The control group was just given the routine treatment. Both groups were treated for two weeks continuously. The National institute of Health Stroke Scale (NIHSS ) and Barthel index (BI) were measured before and after the treatment，the adverse reactions were evaluated. Results The scoreing of NIHSS and BI in treatment group were much higher than control group(P<0.05).The different of adverse reactions in both groups were not significant (P > 0.05 ). Conclusions The treatment of progressive stroke with hydroxyethyl starch combined Edaravone has sure curative effect, and no significant adverse reactions. Related Articles | Metrics

临床论丛

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The clinic study of prevention of lower limb deep vein thrombosis by low molecular heparin after abdominal operation 2012, 40 (8):  839-840 .  Abstract ( 893 )   PDF (392KB) ( 2415 )   【Abstract Objective】To explore the role of low molecular heparin in prevention of lower limb deep vein thrombosis (DVT) after abdominal operation. Methods we selected 70 cases who underwent abdominal operations, pre-operation exam included blood coagulation function, D2 polymer, bilateral low limbs vascular Doppler scanning. Those with thrombosis were excluded. The cases receive low molecular heparin were treatment group, which was given low molecular heparin 4100u, priumbilical subcutaneous injection, one daily, all for 3-5days. The rest without receiving low molecular heparin were the observation group. In post-operative day 7 or 8, all cases received bilateral low limbs vascular Doppler scanning. In both group, the rate of DVT and wound oozing were compared. Result the rate of DVT in treatment group was much lower than that of conversation group(P<0.05), the rate of wound oozing had no obvious difference. Conclusion in prevention of low limb deep vein thrombosis the low molecular heparin acts actively, we should pay more attention. Related Articles | Metrics

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Pilot Study of the Flotrac/Vigileo? System for Haemodynamic Parameters Monitoring in Patients Undergoing Cesarean Section 2012, 40 (8):  840-842 .  Abstract ( 1407 )   PDF (395KB) ( 2432 )   Objective: The aim of this study was to evaluate cardiac output and other hemodynamic measurements made using the FloTrac/VigileoTM system in patients undergoing spinal anesthesia for elective cesarean section. Methods: A prospective study enrolling 19 healthy pregnant women was performed. Hemodynamic parameters were continuously obtained at 14 main points: admission to surgery (two baseline measurements), after preload, after spinal anesthesia administration and 4 time points thereafter (4, 6, 8 and 10 min after anesthesia), at skin and uterine incision, newborn and placental delivery, oxytocin administration, end of surgery. Hemodynamic therapy was guided by mean arterial pressure, and vasopressors were used as appropriate to maintain baseline values. A repeated measures ANOVA was used for data analysis. Results: There was a significant decrease of MAP up to 4 min after spinal anesthesia (P<0.05). And heart rate increased with MAP decreased after newborn and placental delivery. Importantly, stroke volume variation decreased after preload and oxytocin administration (P< 0.05) and returned to basal values at the end of surgery. Further hemodynamic parameters showed no significant changes over time. Conclusion: No significant hemodynamic effects, except for heart rate and stroke volume changes, were observed in pregnant women managed with preload and vasopressors when undergoing elective cesarean section and spinal anesthesia. Related Articles | Metrics

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The Relationship between Serum Cystatin C and Cardiac Function in Patients with Chronic Heart Failure 2012, 40 (8):  845-846 .  Abstract ( 828 )   PDF (380KB) ( 2352 )   Abstract Objective：To investigate the relationship between serum Cystatin C (CysC) and cardiac function in patients with chronic heart failure(CHF). Methods：193 patients to be hospitalized over 2 weeks with CHF were colleced . Left ventricular end-diastolic diameter (LVEDD),left ventricular end-systolic diameter(LVESD) and left ventricular ejection fraction(LVEF)were measured by echocardiography before and 2 weeks after therapy.The level of serum CysC and 6-minute walk test(6-MWT) were determined too.Their results and relationship were compared. Results：The level of serum CysC decreased significantly(P <0.05) after therapy.The relationship between serum CysC and LVEDD、LVESD、LVEF、6-MWT was negative correlation (P <0.05).Conclusions：There were close relationship between CysC and CHF. CysC was a potential biomarker in reflect the cardiac function of patients with CHF. Related Articles | Metrics

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CT and MRI of Pediatric Orbit Tumors and Tumorlike Lesions wan-hua GUO 2012, 40 (8):  849-850 .  Abstract ( 815 )   PDF (1065KB) ( 2607 )   【Abstract】Objective: To analyze the CT and MRI of pediatric orbit tumors and tumorlike lesions. Methods: CT and MRI findings of 49 children with pathologically or clinically proved orbital tumor were retrospectively analyzed. Results: 36 cases are osseous lesions of the orbit, including 19 dermoid cysts, 4 fibrous dysplasia, 2 Langerhans cell histiocytosis, 4 granulocytic sarcoma, and 7 neuroblastoma Metastases. 13 cases are nonosseous lesions of the extraocular orbit, including 9 Infantile Hemangiomas and 4 Neurofibromatosis type 1. Conclusion: CT and MRI can be useful to define the extent of the lesion, narrow the differential diagnosis, have important value to the following treatment. Related Articles | Metrics

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The value of HRCT in the diagnosis of malformation of inner ear 2012, 40 (8):  851-852 .  Abstract ( 1134 )   PDF (2274KB) ( 2449 )   Abstract: Objective To evaluate malformation of inner ear using HRCT. Methods 35 patients diagnosed malformation of inner ear radiological were collected in our study from Mar 2008 to Mar 2010. Results 8 cases had cochlea malformation ;18 cases had malformation of enlargement of vestibular aqueduct ; 2 case had vestibular malformation with enlargement of vestibular aqueduct , 7 cases had abnormal internal auditory canal. Conclusion HRCT is a good modality to show the malformation of inner ear. Related Articles | Metrics

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The analysis of HIV and Syphilitic detection results of 4318 serum samples in venereal disease out-patient clinic 2012, 40 (8):  853-854 .  Abstract ( 1078 )   PDF (360KB) ( 2418 )   Abstract：Objective To master the prevalence condition of HIV and syphilis, the detection status of HIV and syphilis in the laboratory of our hospital’s venereal disease out-patient clinic was analyzed. Methods TPPA and RPR were used to detect syphilis, and positive percentage of first diagnosis was calculated. Meanwhile, ELISA and latex chromatographic analysis test were used to detect anti-HIV antibody of all serum specimens. Results 3554 patients were detected syphilis， the result of 1168 patients was positive (32.7%) .The positive rate of male and female is 16.6% and 16.1% respectively. The positive rate of female is higher than that of male in 21~30 age group ,while male’s positive rate is higher than female’s in other group. 4318 samples were screened anti-HIV antibody, the result of 31 patients was positive, including 1 female whose syphilis result was negative , and 30 male . Among 30 anti-HIV antibody positive male patients, 22 patients were infected by syphilis at the same time, and all patients had homosexuality history. 21-30 group had maximum detected patients and syphilis、HIV infected patients. Discussion The positive rate of syphilis transmitted by sex was in a higher level, which maybe be associated with multiple sexual partners among men who have sex with men (MSM), and unprotected sexual behavior .MSM increase the co-infection rate of syphilis and HIV, which indicate the relative department enact correlated policy for different susceptible population, and control the popular tendency of syphilis and HIV. Related Articles | Metrics

论著

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Analysis the predictive value in early mortality and postoperative complications in patients undergoing coronary artery bypass grafting in a single heart center by SinoSCORE 2012, 40 (8):  1114-1117 .  Abstract ( 924 )   PDF (169KB) ( 2391 )   Objective: To predict the early mortality and postoperative complication in patients undergoing coronary artery bypass grafting（CABG）by SinoSCORE in a single heart center,and access the performance of the SinoSCORE. Methods: To collect the clinical information of patients undergoing CABG in our department. Tne SinoSCORE was used to predict early mortality,access the performance of SinoSCORE predict the early mortality through compare the predictive mortality and the observed mortality.To use Hosmer-Lemeshow goodness-of-fit test access the calibration. Discrimination was tested by determining the area under the receiver operating characteristic(ROC) curve(AUC). To evaluate the performance of SinoSCORE on postoperative complications by AUC and Hosmer-Lemeshow test.The optimal cut—off point for SinoSCORE predicting major complications was obtained by the Yonden index．To used X2 test to Compare incidence rate of postoperative complications in two group divided by cut—off point. Results: The overall mean baseline age was(62.8±8.8)years. The observed mortality in postoperative 30 days of all our study patients was 1.99％(22／1103)．The predictive mortality of SinoSCORE was 3.01%. The predicted mortality calculated by the SinoSCORE was 3.01% which was slightly higher than the actual mortality.To use Hosmer-Lemeshow goodness-of-fit test access the calibration: X2=3.473，P=0.748，P＞0.05.The text Proved SinoSCORE had a good calibration for postoperative early mortality. Discrimination was tested by ROC, AUC=0.751＞0.75，that means SinoSCORE showed high discriminatory ability in predicting mortality.SinoSCORE also showed the good calibration power and vary high discriminatory ability for the main complication risk evaluation such as Low cardiac output syndrome, cerebrovascular events,tracheal incision ,MODS and intraoperative/postoperative IABP implantation. Hosmer-Lemeshow: P=0.415，P=0.689，P=0.243，P=0.945，P=0.287. Areas under ROC curve was AUC=0.785，AUC=0.745，AUC=0.829，AUC=0.821，AUC=0.757.Obtained the optimal Cut-off points by calculating the Youden index. The optimal cut-off points for SinoSCORE model predicting early mortality and the above five major postoperative complication was 3.5，6.5，5.5，3.5，3.5，5.5.Conclusion: SinoSCOBE have a good forecast performance for the postoperative early mortality and some postoperative complication in the patients undergoing CABG in our department.And the postoperative complication include Low cardiac output syndrome,cerebrovascular events, tracheal incision and intraoperative/postoperative IABP implantation. Related Articles | Metrics